AlfalfaGEDB | Msa0224700

M. sativa cultivar zhongmu-4 / Msa0224700

KEGG: -
GO: GO:0005575 GO:0005576 GO:0048046
PFAM: PF00657.25

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0224700	XP_003597654.1	88.757	169	4	1	1	154	186	354	2.75e-99	300

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0224700	sp\|Q9LU14\|APG2_ARATH	66.071	168	42	1	2	154	186	353	5.01e-74	228

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0224700	G7IHX6	88.757	169	4	1	1	154	186	354	1.31e-99	300

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000220	Msa0224700	0.809705	1.657948e-50	-8.615850e-47
Msa0003370	Msa0224700	0.805399	1.360046e-49	-8.615850e-47
Msa0009210	Msa0224700	0.815876	7.384998e-52	-8.615850e-47
Msa0010460	Msa0224700	-0.801205	1.005162e-48	-8.615850e-47
Msa0018220	Msa0224700	0.826223	3.052422e-54	-8.615850e-47
Msa0026120	Msa0224700	0.801925	7.154593e-49	-8.615850e-47
Msa0028450	Msa0224700	0.802068	6.683988e-49	-8.615850e-47
Msa0030710	Msa0224700	0.816543	5.237713e-52	-8.615850e-47
Msa0032040	Msa0224700	0.801124	1.044012e-48	-8.615850e-47
Msa0032340	Msa0224700	-0.833116	6.413872e-56	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0224700	MtrunA17_Chr2g0330781	88.757	169	4	1	1	154	186	354	2.53e-103	300
Msa0224700	MtrunA17_Chr8g0341001	72.024	168	32	1	1	153	187	354	2.37e-83	249
Msa0224700	MtrunA17_Chr2g0320671	55.689	167	58	2	2	153	186	351	2.47e-61	192
Msa0224700	MtrunA17_Chr4g0027571	51.497	167	65	2	2	153	56	221	1.12e-57	179
Msa0224700	MtrunA17_Chr7g0229581	55.034	149	51	2	20	153	7	154	5.01e-55	170
Msa0224700	MtrunA17_Chr4g0018571	53.642	151	54	2	18	153	28	177	2.60e-49	156
Msa0224700	MtrunA17_Chr4g0027521	44.970	169	76	2	2	153	183	351	7.55e-47	155
Msa0224700	MtrunA17_Chr6g0469521	52.033	123	43	2	20	127	31	152	1.35e-38	128
Msa0224700	MtrunA17_Chr3g0105401	40.854	164	81	2	2	150	185	347	1.37e-38	134
Msa0224700	MtrunA17_Chr5g0438621	40.000	160	80	2	2	146	186	344	2.93e-36	127

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0224700	AT3G16370.1	66.071	168	42	1	2	154	186	353	5.09e-75	228
Msa0224700	AT5G22810.1	55.689	167	58	2	2	153	193	358	1.86e-64	201
Msa0224700	AT5G03810.1	55.952	168	57	2	2	153	186	352	1.41e-62	196
Msa0224700	AT5G03820.1	56.213	169	56	2	2	153	189	356	6.60e-62	194
Msa0224700	AT5G03820.2	56.213	169	56	2	2	153	217	384	1.69e-61	194
Msa0224700	AT3G53100.1	48.503	167	70	2	2	153	185	350	4.49e-54	174
Msa0224700	AT1G59406.1	43.827	162	73	4	6	151	187	346	1.10e-40	139
Msa0224700	AT1G59030.1	43.827	162	73	4	6	151	187	346	1.10e-40	139
Msa0224700	AT1G58725.1	43.827	162	73	4	6	151	187	346	1.10e-40	139
Msa0224700	AT5G42170.1	39.506	162	82	2	5	151	206	366	5.56e-38	133

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Find 45 sgRNAs with CRISPR-Local

Find 141 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTCCAAAAGCAACTTCC+TGG	0.409834	2_1:+74591176	Msa0224700:CDS
AACATAGGACTTGTATAAAT+TGG	0.146657	2_1:+74591004	Msa0224700:intron
AACTTGAGTATGTATCAACA+AGG	0.714639	2_1:-74590826	None:intergenic
AAGACAACAATCTTAAGACC+AGG	0.651941	2_1:-74591194	None:intergenic
AATCTTGGTTAATTTCACTA+AGG	0.476359	2_1:-74591763	None:intergenic
ACATAGGACTTGTATAAATT+GGG	0.401221	2_1:+74591005	Msa0224700:intron
ACTTGGTTAGCAGCTTCTGA+AGG	0.578036	2_1:-74591491	None:intergenic
AGAACATTGAACTCCGAATT+CGG	0.339968	2_1:+74591834	Msa0224700:three_prime_UTR
AGAATCAACAATGATGCACA+AGG	0.655662	2_1:+74591122	Msa0224700:CDS
AGATAACACAATGCGAATCT+TGG	0.465295	2_1:-74591778	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACTTGAGTATGTATCAACA+AGG	-	chr2_1:74590829-74590848	None:intergenic	30.0%
TTATTTGTAACGATGTGGTA+TGG	-	chr2_1:74590958-74590977	None:intergenic	30.0%
AAATTGGGAGCTAGAAAAAT+TGG	+	chr2_1:74591020-74591039	Msa0224700:CDS	30.0%
TCTATCTTGTGACATTCAAT+AGG	+	chr2_1:74591341-74591360	Msa0224700:intron	30.0%
ATGTACTTAAACAAGTCACT+AGG	-	chr2_1:74591651-74591670	None:intergenic	30.0%
TTGATTAGCATAATGACAGA+TGG	-	chr2_1:74591814-74591833	None:intergenic	30.0%
AACTTGAGTATGTATCAACA+AGG	-	chr2_1:74590829-74590848	None:intergenic	30.0%
TTATTTGTAACGATGTGGTA+TGG	-	chr2_1:74590958-74590977	None:intergenic	30.0%
AAATTGGGAGCTAGAAAAAT+TGG	+	chr2_1:74591020-74591039	Msa0224700:CDS	30.0%
TCTATCTTGTGACATTCAAT+AGG	+	chr2_1:74591341-74591360	Msa0224700:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	74590800	74591868	74590800	ID=Msa0224700;Name=Msa0224700
chr2_1	mRNA	74590800	74591868	74590800	ID=Msa0224700-mRNA-1;Parent=Msa0224700;Name=Msa0224700-mRNA-1;_AED=0.12;_eAED=0.12;_QI=0\|0.33\|0.25\|1\|0.33\|0.25\|4\|309\|154
chr2_1	exon	74590800	74590856	74590800	ID=Msa0224700-mRNA-1:exon:22158;Parent=Msa0224700-mRNA-1
chr2_1	exon	74591011	74591065	74591011	ID=Msa0224700-mRNA-1:exon:22159;Parent=Msa0224700-mRNA-1
chr2_1	exon	74591111	74591266	74591111	ID=Msa0224700-mRNA-1:exon:22160;Parent=Msa0224700-mRNA-1
chr2_1	exon	74591363	74591868	74591363	ID=Msa0224700-mRNA-1:exon:22161;Parent=Msa0224700-mRNA-1
chr2_1	CDS	74590800	74590856	74590800	ID=Msa0224700-mRNA-1:cds;Parent=Msa0224700-mRNA-1
chr2_1	CDS	74591011	74591065	74591011	ID=Msa0224700-mRNA-1:cds;Parent=Msa0224700-mRNA-1
chr2_1	CDS	74591111	74591266	74591111	ID=Msa0224700-mRNA-1:cds;Parent=Msa0224700-mRNA-1
chr2_1	CDS	74591363	74591559	74591363	ID=Msa0224700-mRNA-1:cds;Parent=Msa0224700-mRNA-1
chr2_1	three_prime_UTR	74591560	74591868	74591560	ID=Msa0224700-mRNA-1:three_prime_utr;Parent=Msa0224700-mRNA-1

Gene Sequence

>Msa0224700

GTCACTCCTGATCAATATTCTGCATACCTTGTTGATACATACTCAAGTTTTGTTAAGGACTTGTATAAATTGGGAGCTAGAAAAATTGGAGTGACTTCACTACCACCATTGGGTTGTGTCACCAGAATCAACAATGATGCACAAGGATTTAACAAGAAAATCAACTCGGCTACAGCGAAACTCCAAAAGCAACTTCCTGGTCTTAAGATTGTTGTCTTTAATATTTACAAACCTCTCTATGAACTTGTTCAATCCCCTTCAAAATTTGGTTTTGCGGAGGCAAGGAAAGGCTGCTGTGGAACTGGGATTGTAGAGACGACATCCTTATTGTGCAATCAAAAGTCACCAGGAACCTGTTCTAATGCAACTCAGTATGTGTTTTGGGACAGTGTCCATCCTTCAGAAGCTGCTAACCAAGTTCTAGCTGATGCATTGATTGAACAAGGCATAAGCCTTATCGGTTAA

Protein sequence

>Msa0224700

VTPDQYSAYLVDTYSSFVKDLYKLGARKIGVTSLPPLGCVTRINNDAQGFNKKINSATAKLQKQLPGLKIVVFNIYKPLYELVQSPSKFGFAEARKGCCGTGIVETTSLLCNQKSPGTCSNATQYVFWDSVHPSEAANQVLADALIEQGISLIG*