AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1214730


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1214730 MtrunA17_Chr4g0063931 96.581 117 4 0 1 117 75 191 1.05e-81 237
Msa1214730 MtrunA17_Chr2g0322221 86.154 65 9 0 37 101 117 181 5.66e-38 126
Msa1214730 MtrunA17_Chr4g0025361 78.462 65 9 1 37 101 105 164 6.76e-31 108
Msa1214730 MtrunA17_Chr4g0011841 63.736 91 31 1 3 91 96 186 1.09e-30 108
Msa1214730 MtrunA17_Chr4g0056521 88.462 52 6 0 40 91 130 181 3.16e-29 105
Msa1214730 MtrunA17_Chr5g0413381 78.431 51 11 0 34 84 112 162 3.71e-25 94.0
Msa1214730 MtrunA17_Chr5g0419871 69.388 49 15 0 35 83 107 155 9.35e-18 74.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1214730 AT2G26580.2 76.531 98 18 1 3 100 71 163 7.36e-49 153
Msa1214730 AT2G26580.1 76.531 98 18 1 3 100 71 163 7.36e-49 153
Msa1214730 AT1G08465.1 85.714 63 9 0 37 99 107 169 7.61e-36 121
Msa1214730 AT2G45190.2 92.982 57 4 0 35 91 70 126 1.04e-34 117
Msa1214730 AT2G45190.1 92.982 57 4 0 35 91 134 190 3.19e-34 118
Msa1214730 AT4G00180.2 90.741 54 5 0 40 93 120 173 2.51e-31 110
Msa1214730 AT4G00180.1 90.741 54 5 0 40 93 151 204 5.03e-31 110
Msa1214730 AT1G23420.1 74.545 55 14 0 33 87 126 180 1.59e-24 93.6
Msa1214730 AT1G23420.2 74.545 55 14 0 33 87 157 211 3.64e-24 93.2
Msa1214730 AT1G69180.1 69.388 49 15 0 35 83 108 156 4.18e-20 80.9

Find 35 sgRNAs with CRISPR-Local

Find 108 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTGCTTACTACTTGTTTAA+TGG 0.130290 8_1:-75479959 Msa1214730:three_prime_UTR
TATAATAGACTAGCCAAATT+TGG 0.202022 8_1:+75479842 None:intergenic
TGCAATCCTATCGTTGCATT+TGG 0.296610 8_1:+75480971 None:intergenic
GATGATCCGGAATCCAAATT+TGG 0.301410 8_1:-75479855 Msa1214730:three_prime_UTR
GACTAGCCAAATTTGGATTC+CGG 0.350145 8_1:+75479849 None:intergenic
GCTTCTCTGTGACTGATATC+AGG 0.363234 8_1:+75480455 None:intergenic
GCAATCCTATCGTTGCATTT+GGG 0.399881 8_1:+75480972 None:intergenic
AGGTACACGTTGCCTCTTCT+CGG 0.458670 8_1:+75480650 None:intergenic
ATCAGCCCAAAATGAATATG+AGG 0.476928 8_1:+75480314 None:intergenic
CACCCATGTTACTGAGGAAA+GGG 0.483814 8_1:-75480931 Msa1214730:CDS
ATTAAAGATAGAAACTGATA+AGG 0.484078 8_1:+75479891 None:intergenic
GAGCAACAATCAAGCTAAGA+TGG 0.499438 8_1:-75480285 Msa1214730:CDS
TCCTCAGTAACATGGGTGGT+TGG 0.501150 8_1:+75480936 None:intergenic
ACTGCATGAATCCAGAGTAT+AGG 0.501971 8_1:-75481013 Msa1214730:exon
GGTACACGTTGCCTCTTCTC+GGG 0.503039 8_1:+75480651 None:intergenic
CAACCCTTTCCTCAGTAACA+TGG 0.504380 8_1:+75480928 None:intergenic
TTGTTCATAAGATGTTAACT+AGG 0.512933 8_1:-75480100 Msa1214730:three_prime_UTR
TGAGAAGCGTTTGATGTCTA+CGG 0.515736 8_1:-75480154 Msa1214730:CDS
ATATAGATAGATAGATGATC+CGG 0.525158 8_1:-75479868 Msa1214730:three_prime_UTR
TCCTTAACTCATGAAAAGTC+AGG 0.525284 8_1:+75480033 None:intergenic
CCTATCGTTGCATTTGGGAG+TGG 0.533061 8_1:+75480977 None:intergenic
AATTTCCAGCTCCCGAGAAG+AGG 0.540719 8_1:-75480662 Msa1214730:intron
TCTGGATTCATGCAGTGGCT+AGG 0.562771 8_1:+75481020 None:intergenic
CTAGTTCGAATCCTATACTC+TGG 0.567724 8_1:+75481002 None:intergenic
GGAAGAGATTCAGAGGATCA+AGG 0.576711 8_1:-75480486 Msa1214730:CDS
CCACCCATGTTACTGAGGAA+AGG 0.581614 8_1:-75480932 Msa1214730:CDS
AGAAACTGATAAGGATGACA+AGG 0.582761 8_1:+75479900 None:intergenic
ATGAACTGATTATAAGCAGA+AGG 0.586762 8_1:+75480630 None:intergenic
CCACTCCCAAATGCAACGAT+AGG 0.592244 8_1:-75480977 Msa1214730:CDS
TATGCAGGGAAGAGATTCAG+AGG 0.621557 8_1:-75480493 Msa1214730:intron
AACCCTTTCCTCAGTAACAT+GGG 0.626838 8_1:+75480929 None:intergenic
TATACTCTGGATTCATGCAG+TGG 0.629931 8_1:+75481015 None:intergenic
ACCAACCACCCATGTTACTG+AGG 0.669098 8_1:-75480937 Msa1214730:CDS
CCTTTCCTCAGTAACATGGG+TGG 0.674444 8_1:+75480932 None:intergenic
GAAACTGATAAGGATGACAA+GGG 0.679298 8_1:+75479901 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTATATTTATATATATGC+AGG - chr8_1:75480353-75480372 Msa1214730:intron 10.0%
!! TTTATATTTATATATATGCA+GGG - chr8_1:75480354-75480373 Msa1214730:intron 10.0%
!!! ATTATTTCTTTATTTTTTAG+TGG - chr8_1:75480515-75480534 Msa1214730:intron 10.0%
!!! TTATTTCTTTATTTTTTAGT+GGG - chr8_1:75480516-75480535 Msa1214730:intron 10.0%
!!! TGATTATTTTTATTATATTC+AGG - chr8_1:75480680-75480699 Msa1214730:intron 10.0%
!!! TTTTATATTTATATATATGC+AGG - chr8_1:75480353-75480372 Msa1214730:intron 10.0%
!! TTTATATTTATATATATGCA+GGG - chr8_1:75480354-75480373 Msa1214730:intron 10.0%
!!! ATTATTTCTTTATTTTTTAG+TGG - chr8_1:75480515-75480534 Msa1214730:intron 10.0%
!!! TTATTTCTTTATTTTTTAGT+GGG - chr8_1:75480516-75480535 Msa1214730:intron 10.0%
!!! TGATTATTTTTATTATATTC+AGG - chr8_1:75480680-75480699 Msa1214730:intron 10.0%
!! TTTAAATAGATAGGAAAAAA+AGG + chr8_1:75479995-75480014 None:intergenic 15.0%
!! TTTAAATAGATAGGAAAAAA+AGG + chr8_1:75479995-75480014 None:intergenic 15.0%
!! AATTTAGAAAATAAACTACG+TGG + chr8_1:75480097-75480116 None:intergenic 20.0%
!!! GTATATATAGTTGATTCAAT+TGG - chr8_1:75480293-75480312 Msa1214730:CDS 20.0%
!!! ATTTTCCTCATATTCATTTT+GGG - chr8_1:75480542-75480561 Msa1214730:intron 20.0%
!! ATTAAAGATAGAAACTGATA+AGG + chr8_1:75480973-75480992 None:intergenic 20.0%
!! AATTTAGAAAATAAACTACG+TGG + chr8_1:75480097-75480116 None:intergenic 20.0%
!!! GTATATATAGTTGATTCAAT+TGG - chr8_1:75480293-75480312 Msa1214730:CDS 20.0%
!!! ATTTTCCTCATATTCATTTT+GGG - chr8_1:75480542-75480561 Msa1214730:intron 20.0%
!! ATTAAAGATAGAAACTGATA+AGG + chr8_1:75480973-75480992 None:intergenic 20.0%
!! TAGTATTTCTATGTTCGTTA+AGG + chr8_1:75480129-75480148 None:intergenic 25.0%
!! CATTTTCCTCATATTCATTT+TGG - chr8_1:75480541-75480560 Msa1214730:intron 25.0%
! TTGTTCATAAGATGTTAACT+AGG - chr8_1:75480761-75480780 Msa1214730:intron 25.0%
! ATATAGATAGATAGATGATC+CGG - chr8_1:75480993-75481012 Msa1214730:CDS 25.0%
!! TATAATAGACTAGCCAAATT+TGG + chr8_1:75481022-75481041 None:intergenic 25.0%
!! TAGTATTTCTATGTTCGTTA+AGG + chr8_1:75480129-75480148 None:intergenic 25.0%
!! CATTTTCCTCATATTCATTT+TGG - chr8_1:75480541-75480560 Msa1214730:intron 25.0%
! TTGTTCATAAGATGTTAACT+AGG - chr8_1:75480761-75480780 Msa1214730:intron 25.0%
! ATATAGATAGATAGATGATC+CGG - chr8_1:75480993-75481012 Msa1214730:CDS 25.0%
!! TATAATAGACTAGCCAAATT+TGG + chr8_1:75481022-75481041 None:intergenic 25.0%
! GAGAGTATATAGTAAACTCA+CGG + chr8_1:75479966-75479985 None:intergenic 30.0%
ATGAACTGATTATAAGCAGA+AGG + chr8_1:75480234-75480253 None:intergenic 30.0%
ACTGCTTACTACTTGTTTAA+TGG - chr8_1:75480902-75480921 Msa1214730:intron 30.0%
! GAGAGTATATAGTAAACTCA+CGG + chr8_1:75479966-75479985 None:intergenic 30.0%
ATGAACTGATTATAAGCAGA+AGG + chr8_1:75480234-75480253 None:intergenic 30.0%
ACTGCTTACTACTTGTTTAA+TGG - chr8_1:75480902-75480921 Msa1214730:intron 30.0%
AGTATATAGTAAACTCACGG+CGG + chr8_1:75479963-75479982 None:intergenic 35.0%
TAAACTACGTGGCTATGATA+TGG + chr8_1:75480086-75480105 None:intergenic 35.0%
ATCAGCCCAAAATGAATATG+AGG + chr8_1:75480550-75480569 None:intergenic 35.0%
! TATTCATTTTGGGCTGATGT+TGG - chr8_1:75480552-75480571 Msa1214730:intron 35.0%
! TCCTGACTTTTCATGAGTTA+AGG - chr8_1:75480827-75480846 Msa1214730:intron 35.0%
TCCTTAACTCATGAAAAGTC+AGG + chr8_1:75480831-75480850 None:intergenic 35.0%
GAAACTGATAAGGATGACAA+GGG + chr8_1:75480963-75480982 None:intergenic 35.0%
AGAAACTGATAAGGATGACA+AGG + chr8_1:75480964-75480983 None:intergenic 35.0%
AGTATATAGTAAACTCACGG+CGG + chr8_1:75479963-75479982 None:intergenic 35.0%
TAAACTACGTGGCTATGATA+TGG + chr8_1:75480086-75480105 None:intergenic 35.0%
ATCAGCCCAAAATGAATATG+AGG + chr8_1:75480550-75480569 None:intergenic 35.0%
! TATTCATTTTGGGCTGATGT+TGG - chr8_1:75480552-75480571 Msa1214730:intron 35.0%
! TCCTGACTTTTCATGAGTTA+AGG - chr8_1:75480827-75480846 Msa1214730:intron 35.0%
TCCTTAACTCATGAAAAGTC+AGG + chr8_1:75480831-75480850 None:intergenic 35.0%
GAAACTGATAAGGATGACAA+GGG + chr8_1:75480963-75480982 None:intergenic 35.0%
AGAAACTGATAAGGATGACA+AGG + chr8_1:75480964-75480983 None:intergenic 35.0%
TATACTCTGGATTCATGCAG+TGG + chr8_1:75479849-75479868 None:intergenic 40.0%
ACTGCATGAATCCAGAGTAT+AGG - chr8_1:75479848-75479867 Msa1214730:three_prime_UTR 40.0%
CTAGTTCGAATCCTATACTC+TGG + chr8_1:75479862-75479881 None:intergenic 40.0%
GCAATCCTATCGTTGCATTT+GGG + chr8_1:75479892-75479911 None:intergenic 40.0%
TGCAATCCTATCGTTGCATT+TGG + chr8_1:75479893-75479912 None:intergenic 40.0%
AACCCTTTCCTCAGTAACAT+GGG + chr8_1:75479935-75479954 None:intergenic 40.0%
GAGCAACAATCAAGCTAAGA+TGG - chr8_1:75480576-75480595 Msa1214730:intron 40.0%
!! TGAGAAGCGTTTGATGTCTA+CGG - chr8_1:75480707-75480726 Msa1214730:intron 40.0%
GATGATCCGGAATCCAAATT+TGG - chr8_1:75481006-75481025 Msa1214730:CDS 40.0%
! GACTAGCCAAATTTGGATTC+CGG + chr8_1:75481015-75481034 None:intergenic 40.0%
TATACTCTGGATTCATGCAG+TGG + chr8_1:75479849-75479868 None:intergenic 40.0%
ACTGCATGAATCCAGAGTAT+AGG - chr8_1:75479848-75479867 Msa1214730:three_prime_UTR 40.0%
CTAGTTCGAATCCTATACTC+TGG + chr8_1:75479862-75479881 None:intergenic 40.0%
GCAATCCTATCGTTGCATTT+GGG + chr8_1:75479892-75479911 None:intergenic 40.0%
TGCAATCCTATCGTTGCATT+TGG + chr8_1:75479893-75479912 None:intergenic 40.0%
AACCCTTTCCTCAGTAACAT+GGG + chr8_1:75479935-75479954 None:intergenic 40.0%
GAGCAACAATCAAGCTAAGA+TGG - chr8_1:75480576-75480595 Msa1214730:intron 40.0%
!! TGAGAAGCGTTTGATGTCTA+CGG - chr8_1:75480707-75480726 Msa1214730:intron 40.0%
GATGATCCGGAATCCAAATT+TGG - chr8_1:75481006-75481025 Msa1214730:CDS 40.0%
! GACTAGCCAAATTTGGATTC+CGG + chr8_1:75481015-75481034 None:intergenic 40.0%
CACCCATGTTACTGAGGAAA+GGG - chr8_1:75479930-75479949 Msa1214730:three_prime_UTR 45.0%
CAACCCTTTCCTCAGTAACA+TGG + chr8_1:75479936-75479955 None:intergenic 45.0%
TATGCAGGGAAGAGATTCAG+AGG - chr8_1:75480368-75480387 Msa1214730:intron 45.0%
GGAAGAGATTCAGAGGATCA+AGG - chr8_1:75480375-75480394 Msa1214730:intron 45.0%
GCTTCTCTGTGACTGATATC+AGG + chr8_1:75480409-75480428 None:intergenic 45.0%
CACCCATGTTACTGAGGAAA+GGG - chr8_1:75479930-75479949 Msa1214730:three_prime_UTR 45.0%
CAACCCTTTCCTCAGTAACA+TGG + chr8_1:75479936-75479955 None:intergenic 45.0%
TATGCAGGGAAGAGATTCAG+AGG - chr8_1:75480368-75480387 Msa1214730:intron 45.0%
GGAAGAGATTCAGAGGATCA+AGG - chr8_1:75480375-75480394 Msa1214730:intron 45.0%
GCTTCTCTGTGACTGATATC+AGG + chr8_1:75480409-75480428 None:intergenic 45.0%
!!! TATTTTATATTTAAATAGAT+AGG + chr8_1:75480004-75480023 None:intergenic 5.0%
!! TAATGATTAAAAAATTAAAT+TGG + chr8_1:75480624-75480643 None:intergenic 5.0%
!!! TATTTTATATTTAAATAGAT+AGG + chr8_1:75480004-75480023 None:intergenic 5.0%
!! TAATGATTAAAAAATTAAAT+TGG + chr8_1:75480624-75480643 None:intergenic 5.0%
TCTGGATTCATGCAGTGGCT+AGG + chr8_1:75479844-75479863 None:intergenic 50.0%
CCTATCGTTGCATTTGGGAG+TGG + chr8_1:75479887-75479906 None:intergenic 50.0%
CCACTCCCAAATGCAACGAT+AGG - chr8_1:75479884-75479903 Msa1214730:three_prime_UTR 50.0%
ACCAACCACCCATGTTACTG+AGG - chr8_1:75479924-75479943 Msa1214730:three_prime_UTR 50.0%
TCCTCAGTAACATGGGTGGT+TGG + chr8_1:75479928-75479947 None:intergenic 50.0%
CCTTTCCTCAGTAACATGGG+TGG + chr8_1:75479932-75479951 None:intergenic 50.0%
CCACCCATGTTACTGAGGAA+AGG - chr8_1:75479929-75479948 Msa1214730:three_prime_UTR 50.0%
AATTTCCAGCTCCCGAGAAG+AGG - chr8_1:75480199-75480218 Msa1214730:intron 50.0%
AGGTACACGTTGCCTCTTCT+CGG + chr8_1:75480214-75480233 None:intergenic 50.0%
TCTGGATTCATGCAGTGGCT+AGG + chr8_1:75479844-75479863 None:intergenic 50.0%
CCTATCGTTGCATTTGGGAG+TGG + chr8_1:75479887-75479906 None:intergenic 50.0%
CCACTCCCAAATGCAACGAT+AGG - chr8_1:75479884-75479903 Msa1214730:three_prime_UTR 50.0%
ACCAACCACCCATGTTACTG+AGG - chr8_1:75479924-75479943 Msa1214730:three_prime_UTR 50.0%
TCCTCAGTAACATGGGTGGT+TGG + chr8_1:75479928-75479947 None:intergenic 50.0%
CCTTTCCTCAGTAACATGGG+TGG + chr8_1:75479932-75479951 None:intergenic 50.0%
CCACCCATGTTACTGAGGAA+AGG - chr8_1:75479929-75479948 Msa1214730:three_prime_UTR 50.0%
AATTTCCAGCTCCCGAGAAG+AGG - chr8_1:75480199-75480218 Msa1214730:intron 50.0%
AGGTACACGTTGCCTCTTCT+CGG + chr8_1:75480214-75480233 None:intergenic 50.0%
GGTACACGTTGCCTCTTCTC+GGG + chr8_1:75480213-75480232 None:intergenic 55.0%
GGTACACGTTGCCTCTTCTC+GGG + chr8_1:75480213-75480232 None:intergenic 55.0%
CGTTGCCTCTTCTCGGGAGC+TGG + chr8_1:75480207-75480226 None:intergenic 65.0%
CGTTGCCTCTTCTCGGGAGC+TGG + chr8_1:75480207-75480226 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 75479835 75481048 75479835 ID=Msa1214730;Name=Msa1214730
chr8_1 mRNA 75479835 75481048 75479835 ID=Msa1214730-mRNA-1;Parent=Msa1214730;Name=Msa1214730-mRNA-1;_AED=0.06;_eAED=0.06;_QI=18|1|1|1|1|1|5|299|117
chr8_1 exon 75479835 75480181 75479835 ID=Msa1214730-mRNA-1:exon:25929;Parent=Msa1214730-mRNA-1
chr8_1 exon 75480280 75480348 75480280 ID=Msa1214730-mRNA-1:exon:25928;Parent=Msa1214730-mRNA-1
chr8_1 exon 75480433 75480508 75480433 ID=Msa1214730-mRNA-1:exon:25927;Parent=Msa1214730-mRNA-1
chr8_1 exon 75480627 75480675 75480627 ID=Msa1214730-mRNA-1:exon:25926;Parent=Msa1214730-mRNA-1
chr8_1 exon 75480919 75481048 75480919 ID=Msa1214730-mRNA-1:exon:25925;Parent=Msa1214730-mRNA-1
chr8_1 five_prime_UTR 75481031 75481048 75481031 ID=Msa1214730-mRNA-1:five_prime_utr;Parent=Msa1214730-mRNA-1
chr8_1 CDS 75480919 75481030 75480919 ID=Msa1214730-mRNA-1:cds;Parent=Msa1214730-mRNA-1
chr8_1 CDS 75480627 75480675 75480627 ID=Msa1214730-mRNA-1:cds;Parent=Msa1214730-mRNA-1
chr8_1 CDS 75480433 75480508 75480433 ID=Msa1214730-mRNA-1:cds;Parent=Msa1214730-mRNA-1
chr8_1 CDS 75480280 75480348 75480280 ID=Msa1214730-mRNA-1:cds;Parent=Msa1214730-mRNA-1
chr8_1 CDS 75480134 75480181 75480134 ID=Msa1214730-mRNA-1:cds;Parent=Msa1214730-mRNA-1
chr8_1 three_prime_UTR 75479835 75480133 75479835 ID=Msa1214730-mRNA-1:three_prime_utr;Parent=Msa1214730-mRNA-1
Gene Sequence

>Msa1214730

ATGAATCCAGAGTATAGGATTCGAACTAGCTCCACTCCCAAATGCAACGATAGGATTGCAATGCGTTCTGCACCAACCACCCATGTTACTGAGGAAAGGGTTGTTAACCGCCCTCCCGAGAAGAGGCAACGTGTACCTTCTGCTTATAATCAGTTCATCAAGGAAGAGATTCAGAGGATCAAGGCTAATAATCCTGATATCAGTCACAGAGAAGCTTTTAGTACAGCTGCAAAGAACTGGGCACATTTTCCTCATATTCATTTTGGGCTGATGTTGGAGAGCAACAATCAAGCTAAGATGGAGACTGTCTCTGAGAAGCGTTTGATGTCTACGGCTGCGCTAATGAACAAATGA

Protein sequence

>Msa1214730

MNPEYRIRTSSTPKCNDRIAMRSAPTTHVTEERVVNRPPEKRQRVPSAYNQFIKEEIQRIKANNPDISHREAFSTAAKNWAHFPHIHFGLMLESNNQAKMETVSEKRLMSTAALMNK*