AlfalfaGEDB | Msa0520730

M. sativa cultivar zhongmu-4 / Msa0520730

KEGG: K15397
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006950 GO:0008150 GO:0009266 GO:0009314 GO:0009409 GO:0009416 GO:0009628 GO:0012505 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0080167
PFAM: PF08392.15

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0520730	QEX52173.1	99.628	537	2	0	1	537	1	537	0.0	1110

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0520730	sp\|Q570B4\|KCS10_ARATH	74.773	551	118	3	3	536	4	550	0.0	868

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0520730	A0A5J6NCI0	99.628	537	2	0	1	537	1	537	0.0	1110

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000220	Msa0520730	0.809204	2.123458e-50	-8.615850e-47
Msa0001540	Msa0520730	0.804040	2.614807e-49	-8.615850e-47
Msa0003490	Msa0520730	0.830451	2.914892e-55	-8.615850e-47
Msa0004350	Msa0520730	0.809587	1.757360e-50	-8.615850e-47
Msa0006580	Msa0520730	0.809441	1.888987e-50	-8.615850e-47
Msa0009210	Msa0520730	0.811263	7.639324e-51	-8.615850e-47
Msa0012460	Msa0520730	0.835406	1.709076e-56	-8.615850e-47
Msa0015370	Msa0520730	0.844838	5.900345e-59	-8.615850e-47
Msa0017060	Msa0520730	0.810355	1.201150e-50	-8.615850e-47
Msa0017120	Msa0520730	0.821130	4.754954e-53	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0520730	MtrunA17_Chr3g0136561	98.918	462	5	0	76	537	443	904	0.0	955
Msa0520730	MtrunA17_Chr1g0166191	58.964	502	194	4	35	536	23	512	0.0	629
Msa0520730	MtrunA17_Chr1g0188051	58.498	506	198	4	31	536	13	506	0.0	629
Msa0520730	MtrunA17_Chr4g0035081	58.020	505	199	5	32	536	11	502	0.0	628
Msa0520730	MtrunA17_Chr4g0063011	56.287	501	207	4	36	536	6	494	0.0	614
Msa0520730	MtrunA17_Chr7g0276251	55.664	512	210	7	32	536	27	528	0.0	599
Msa0520730	MtrunA17_Chr1g0209881	55.311	499	210	5	38	536	5	490	0.0	572
Msa0520730	MtrunA17_Chr2g0282281	53.143	525	222	5	3	527	2	502	0.0	570
Msa0520730	MtrunA17_Chr5g0423521	53.094	501	218	5	38	536	5	490	0.0	555
Msa0520730	MtrunA17_Chr2g0306781	61.478	379	136	2	158	536	31	399	6.72e-174	496

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa0520730	AT2G26250.1	74.773	551	118	3	3	536	4	550	868
Msa0520730	AT1G19440.1	59.486	506	192	5	32	537	23	515	640
Msa0520730	AT2G26640.1	56.886	501	204	4	36	536	15	503	622
Msa0520730	AT2G16280.1	57.058	503	203	5	35	537	22	511	610
Msa0520730	AT1G04220.1	57.228	505	199	5	36	534	20	513	599
Msa0520730	AT1G01120.1	55.010	509	215	5	32	536	28	526	594
Msa0520730	AT1G68530.1	55.936	497	206	5	38	534	6	489	586
Msa0520730	AT1G25450.1	53.521	497	218	5	38	534	1	484	557
Msa0520730	AT4G34510.1	54.898	490	202	5	49	537	15	486	554
Msa0520730	AT5G43760.1	56.024	498	203	6	44	536	34	520	553

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Find 123 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACAAACATCACAACACT+AGG	0.592072	3_4:+73248160	Msa0520730:CDS
AAAATAAGCTGTTCTGAAAA+GGG	0.455046	3_4:-73248196	None:intergenic
AAAGACTTAATTGAAATTGG+TGG	0.514408	3_4:+73248127	Msa0520730:CDS
AAAGAGTACTGAGAAAACAA+AGG	0.671840	3_4:-73248822	None:intergenic
AAATCAGGAAAATTTGATGA+AGG	0.547989	3_4:+73246594	Msa0520730:CDS
AACACAGCAACAATGAAAGA+AGG	0.567448	3_4:+73246705	Msa0520730:CDS
AACCCAATTTCACATACTTA+AGG	0.365618	3_4:-73245577	None:intergenic
AACCTCCTTCTAGGAACCCT+TGG	0.476877	3_4:+73248584	Msa0520730:CDS
AACCTTAAGTATGTGAAATT+GGG	0.472847	3_4:+73245575	Msa0520730:CDS
AACGGAGAACGTCAATGAAC+CGG	0.480789	3_4:-73245513	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTCACAGATTGAAGAAAATC+CGG	-	chr3_4:73245557-73245576	None:intergenic	30.0%
GAACCTTAAGTATGTGAAAT+TGG	+	chr3_4:73245574-73245593	Msa0520730:CDS	30.0%
AACCCAATTTCACATACTTA+AGG	-	chr3_4:73245580-73245599	None:intergenic	30.0%
TACCATTATCTGATAAACCA+TGG	+	chr3_4:73245599-73245618	Msa0520730:CDS	30.0%
TAGTGAACAAATAAACACCA+TGG	-	chr3_4:73245619-73245638	None:intergenic	30.0%
CTAAACACAACAAGTAGAAT+TGG	-	chr3_4:73245644-73245663	None:intergenic	30.0%
ATAGTAATGTCATCAGGAAT+AGG	+	chr3_4:73246639-73246658	Msa0520730:CDS	30.0%
GATATACTTCAATCTAATCC+TGG	+	chr3_4:73246960-73246979	Msa0520730:CDS	30.0%
CTGAAATGGTTGGATTTAAT+TGG	+	chr3_4:73247006-73247025	Msa0520730:CDS	30.0%
GTTGGATTTAATTGGTATCA+AGG	+	chr3_4:73247014-73247033	Msa0520730:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_4	gene	73245259	73248954	73245259	ID=Msa0520730;Name=Msa0520730
chr3_4	mRNA	73245259	73248954	73245259	ID=Msa0520730-mRNA-1;Parent=Msa0520730;Name=Msa0520730-mRNA-1;_AED=0.03;_eAED=0.03;_QI=181\|1\|1\|1\|1\|1\|3\|309\|537
chr3_4	exon	73245259	73245853	73245259	ID=Msa0520730-mRNA-1:exon:20438;Parent=Msa0520730-mRNA-1
chr3_4	exon	73246561	73247189	73246561	ID=Msa0520730-mRNA-1:exon:20439;Parent=Msa0520730-mRNA-1
chr3_4	exon	73248075	73248954	73248075	ID=Msa0520730-mRNA-1:exon:20440;Parent=Msa0520730-mRNA-1
chr3_4	five_prime_UTR	73245259	73245439	73245259	ID=Msa0520730-mRNA-1:five_prime_utr;Parent=Msa0520730-mRNA-1
chr3_4	CDS	73245440	73245853	73245440	ID=Msa0520730-mRNA-1:cds;Parent=Msa0520730-mRNA-1
chr3_4	CDS	73246561	73247189	73246561	ID=Msa0520730-mRNA-1:cds;Parent=Msa0520730-mRNA-1
chr3_4	CDS	73248075	73248645	73248075	ID=Msa0520730-mRNA-1:cds;Parent=Msa0520730-mRNA-1
chr3_4	three_prime_UTR	73248646	73248954	73248646	ID=Msa0520730-mRNA-1:three_prime_utr;Parent=Msa0520730-mRNA-1

Gene Sequence

>Msa0520730

ATGGCTACTAACGAAGGTGACATGTTCTCAGCAGAGATTGTGAATCGTGGGATTGAATCATCGGGTCCGAATGCCGGTTCATTGACGTTCTCCGTTCGTGTTCGGAGACGGTTACCGGATTTTCTTCAATCTGTGAACCTTAAGTATGTGAAATTGGGTTACCATTATCTGATAAACCATGGTGTTTATTTGTTCACTATACCAATTCTACTTGTTGTGTTTAGTGCTGAGGTTGGTAGTCTTAGCAAAGAAGATCTTTGGAAGAAGATTTGGGAAGATGCAACTTATGATCTTGCATCTGTTCTCTCTTCTCTTGCTGTCTTTGTTTTCACTTTCACTCTTTACTTCATGTCAAGGCCACGTCCTATTTATCTCATTGATTTTGCATGCTATCAACCTGATGATGAACTCAAGGTATCTAGGGAGCAATTGATAGAGCTAGCAAGAAAATCAGGAAAATTTGATGAAGGAAGTCTAGAATTCCAGAAGAGGATAGTAATGTCATCAGGAATAGGTGATGAAACATACATTCCAAGATCAGTGATAGCATCAAGTGAAAACACAGCAACAATGAAAGAAGGTAGAGCTGAGGCATCAATGGTTATGTTTGGAGCACTTGATGAACTCTTTGAAAAAACAGGAATTAGACCAAAAGATGTTGGTGTTCTTGTAGTAAATTGTAGCATATTCAACCCAACACCATCATTGTCAGCCATGATTATAAATCATTATAAGATGAGGGGAAATATTTTGAGTTATAATCTTGGTGGTATGGGTTGTAGTGCTGGAATTATTTCTGTTGATTTGGCTAGGGATATACTTCAATCTAATCCTGGTAACTATGCTGTTGTTGTGAGTACTGAAATGGTTGGATTTAATTGGTATCAAGGAAAAGAAAGATCAATGCTTATTCCAAATTGTTTCTTTCGTATGGGTTGTTCTGCTGTTTTGCTCTCTAACCGTCGTCGTGATTTTAGCCGCGCGAAATATCGTCTTGAACACATTGTTCGCACACATAAAGGTGCTGATGATCGTAGTTTCAGGTGTGTGTACCAAGAGGAAGACGATCAGAAATTCAAGGGAATAAAGATAAGCAAAGACTTAATTGAAATTGGTGGTGAAGCTCTCAAAACAAACATCACAACACTAGGACCACTGGTCCTACCCTTTTCAGAACAGCTTATTTTCTTTGCTACATTAGTGTGGAGGAACTTCTTTGGTGGTGGAAAATCTGATAAAAATTCACCATCATCAAACAAGCCATACATTCCTAACTACAAACTAGCTTTTGAGCATTTTTGTGTGCATGCTGCTAGCAAAACCATTCTTGATGAGCTTCAAAAGAATCTTGAATTGAGTGACAAGAACATGGAAGCCTCTAGAATGACGCTGCACCGATTCGGCAACACTTCTAGTAGCAGCATTTGGTATGAACTTGCTTATATGGAAGCTAAGGAAAAAGTTAAAAGAGGAGACCGTGTTTGGCAACTCGCTTTTGGTTCTGGTTTTAAGTGTAACAGTGCTGTTTGGCGTTCTATGCGTCGTGTCACAAAACCTCCTTCTAGGAACCCTTGGCTTGACTGCATTGATGCATATCCTGCTTCTCTCAATTAA

Protein sequence

>Msa0520730

MATNEGDMFSAEIVNRGIESSGPNAGSLTFSVRVRRRLPDFLQSVNLKYVKLGYHYLINHGVYLFTIPILLVVFSAEVGSLSKEDLWKKIWEDATYDLASVLSSLAVFVFTFTLYFMSRPRPIYLIDFACYQPDDELKVSREQLIELARKSGKFDEGSLEFQKRIVMSSGIGDETYIPRSVIASSENTATMKEGRAEASMVMFGALDELFEKTGIRPKDVGVLVVNCSIFNPTPSLSAMIINHYKMRGNILSYNLGGMGCSAGIISVDLARDILQSNPGNYAVVVSTEMVGFNWYQGKERSMLIPNCFFRMGCSAVLLSNRRRDFSRAKYRLEHIVRTHKGADDRSFRCVYQEEDDQKFKGIKISKDLIEIGGEALKTNITTLGPLVLPFSEQLIFFATLVWRNFFGGGKSDKNSPSSNKPYIPNYKLAFEHFCVHAASKTILDELQKNLELSDKNMEASRMTLHRFGNTSSSSIWYELAYMEAKEKVKRGDRVWQLAFGSGFKCNSAVWRSMRRVTKPPSRNPWLDCIDAYPASLN*