AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene042174

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene042174.t1 MTR_4g132300 97.025 437 13 0 1 437 1 437 0.0 875
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene042174.t1 AT1G11800 55.580 448 162 6 2 437 19 441 1.72e-167 478

Find 100 sgRNAs with CRISPR-Local

Find 151 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGAACATCTGATCCCATTT+TGG 0.157885 8.4:+1772183 None:intergenic
ATCATTTAGCAACTCTATAA+TGG 0.245684 8.4:-1772298 MS.gene042174:CDS
CATGATTTGCAGGACCATTT+TGG 0.249570 8.4:+1773609 None:intergenic
TAAAACGATCTAATCGCTTT+TGG 0.250022 8.4:+1771944 None:intergenic
TTGGATTCCTTGGCTACTAA+TGG 0.277561 8.4:+1773402 None:intergenic
AATGGTTGATCAAAATCTTC+AGG 0.279274 8.4:+1773354 None:intergenic
CCACAAGAGAGGGTTGATTC+TGG 0.296289 8.4:-1773270 MS.gene042174:CDS
TAATGATCGCTGGGCAAAAC+AGG 0.307351 8.4:+1771802 None:intergenic
CTTGTCCTGCCCCTCCAAAA+TGG 0.307556 8.4:-1772197 MS.gene042174:CDS
TATAGAGTTGCTAAATGATT+TGG 0.312140 8.4:+1772302 None:intergenic
TGTTTGGTTTCGTGAGGATT+TGG 0.325697 8.4:-1773211 MS.gene042174:CDS
TGGTTTCGCAAAATGTTCAT+AGG 0.362409 8.4:+1773290 None:intergenic
AGTAGCCAAGGAATCCAAAA+AGG 0.367865 8.4:-1773397 MS.gene042174:CDS
ACCTGAAAACAGATAAAATC+AGG 0.375798 8.4:+1773138 None:intergenic
CGACTTCGTCTTCTCCAAAA+TGG 0.377694 8.4:-1773623 MS.gene042174:CDS
TACGATTGCCTGTCAACATT+TGG 0.378788 8.4:+1771971 None:intergenic
GAAGAGTGTGCATGATTTGC+AGG 0.387844 8.4:+1773599 None:intergenic
TTGTCCTGCCCCTCCAAAAT+GGG 0.400592 8.4:-1772196 MS.gene042174:CDS
ATTTACCAGCATACAATAGT+AGG 0.405645 8.4:+1772743 None:intergenic
GCAGAAGAGGAAGAGGGGTG+TGG 0.410577 8.4:+1773654 None:intergenic
TATGTCCAACCAGCTTCATT+TGG 0.412262 8.4:+1772009 None:intergenic
TATGAACTGGGATGACAAAA+AGG 0.414590 8.4:-1772085 MS.gene042174:CDS
ATAAGCAATATTGACATGAT+TGG 0.417745 8.4:-1771898 MS.gene042174:CDS
GGAAGAGGGGTGTGGAGGTG+AGG 0.422893 8.4:+1773662 None:intergenic
TCATTTAGCAACTCTATAAT+GGG 0.436917 8.4:-1772297 MS.gene042174:CDS
TAAGCAATATTGACATGATT+GGG 0.440110 8.4:-1771897 MS.gene042174:CDS
AACAAAGAGAAGAAAGTAAG+AGG 0.444004 8.4:-1771847 MS.gene042174:CDS
ATCGATGATGATTCTTCCGT+TGG 0.444069 8.4:-1773492 MS.gene042174:CDS
ATGGTTGATCAGAATCTTCA+AGG 0.445069 8.4:+1773421 None:intergenic
CACAAGAGAGGGTTGATTCT+GGG 0.445307 8.4:-1773269 MS.gene042174:CDS
CAGCAAGGAACGGGTGGAGC+AGG 0.457345 8.4:-1772163 MS.gene042174:CDS
GATATCGTTGAAGCTGGAAA+TGG 0.464894 8.4:+1773521 None:intergenic
ATGGTTGATCAAAATCTTCA+GGG 0.465305 8.4:+1773355 None:intergenic
AAGACGGGTGGCTTGATGCT+TGG 0.467239 8.4:-1772044 MS.gene042174:CDS
GAAGAGGAAGAGGGGTGTGG+AGG 0.471511 8.4:+1773657 None:intergenic
AGAACTTTGCATTGCTGAAG+TGG 0.472526 8.4:-1772271 MS.gene042174:CDS
TCAGATGTTCAGCAAGGAAC+GGG 0.477713 8.4:-1772172 MS.gene042174:CDS
AGGATTTGGAGTTGGAGAAA+AGG 0.480889 8.4:-1773197 MS.gene042174:CDS
TTGTTCAGTTTCTTCTGAGA+AGG 0.481462 8.4:-1772778 MS.gene042174:CDS
AGAAGTGGCAGAAGAGGAAG+AGG 0.481581 8.4:+1773647 None:intergenic
GTCAATATCCTTTACAAGAC+GGG 0.488031 8.4:-1772059 MS.gene042174:CDS
GTTTCGTGAGGATTTGGAGT+TGG 0.489308 8.4:-1773205 MS.gene042174:CDS
TACCGCAGAGGTGGCAAGTA+GGG 0.491308 8.4:+1773562 None:intergenic
AAGAGGCGAGATCAAACAAC+TGG 0.492477 8.4:-1771830 MS.gene042174:CDS
TTCAATCATCTCCAATGTCT+TGG 0.494355 8.4:-1773749 None:intergenic
AGGCTCTGAACATCCTCAAA+AGG 0.495956 8.4:-1772134 MS.gene042174:CDS
GTCGTTGATATCGTTGAAGC+TGG 0.496356 8.4:+1773515 None:intergenic
AGAGTTGCTAAATGATTTGG+TGG 0.498698 8.4:+1772305 None:intergenic
TAAAAGCCAAGGATTCCAAA+AGG 0.500787 8.4:-1773332 MS.gene042174:CDS
CATTCAGATATTTGAGAAGG+TGG 0.508281 8.4:+1773699 None:intergenic
GAGAAAAGGATGAAAGCTAT+TGG 0.509621 8.4:-1773183 MS.gene042174:CDS
TTATCTCGAAATCACGTAAA+CGG 0.510151 8.4:+1771917 None:intergenic
CCAGAATCAACCCTCTCTTG+TGG 0.511191 8.4:+1773270 None:intergenic
AAGGAGACCATAATGATCGC+TGG 0.512582 8.4:+1771792 None:intergenic
CATTGCTGAAGTGGAGGATG+TGG 0.520787 8.4:-1772262 MS.gene042174:CDS
TGATCAACCATTAAAAGCCA+AGG 0.522361 8.4:-1773343 MS.gene042174:CDS
ACTTGCTAGAAAGTGTCAAA+AGG 0.527755 8.4:+1771773 None:intergenic
TCAAGCCACCCGTCTTGTAA+AGG 0.536203 8.4:+1772051 None:intergenic
ACGGGTGGAGCAGGCTAATG+AGG 0.537306 8.4:-1772154 MS.gene042174:CDS
AAGTGGCAGAAGAGGAAGAG+GGG 0.538585 8.4:+1773649 None:intergenic
TGTCAACATTTGGTTTGACT+TGG 0.538956 8.4:+1771981 None:intergenic
AGTCGAAGAAGTGGCAGAAG+AGG 0.541725 8.4:+1773641 None:intergenic
AAAAGCCTACTATTGTATGC+TGG 0.550331 8.4:-1772748 MS.gene042174:intron
AAGTCAAACCAAATGTTGAC+AGG 0.552513 8.4:-1771979 MS.gene042174:CDS
AGAAGACGAAGTCGAAGAAG+TGG 0.554865 8.4:+1773632 None:intergenic
GTACTAAGACCAAATGAAGC+TGG 0.556624 8.4:-1772018 MS.gene042174:CDS
AAAAGCCAAGGATTCCAAAA+GGG 0.562065 8.4:-1773331 MS.gene042174:CDS
ATCAGATGTTCAGCAAGGAA+CGG 0.564097 8.4:-1772173 MS.gene042174:CDS
AGGAGACCATAATGATCGCT+GGG 0.564481 8.4:+1771793 None:intergenic
TGATCAACCATTAGTAGCCA+AGG 0.564807 8.4:-1773409 MS.gene042174:CDS
AGGCCAGAAAACAGAACCAA+CGG 0.565090 8.4:+1773476 None:intergenic
GAGAGGGTTGATTCTGGGAA+AGG 0.567479 8.4:-1773264 MS.gene042174:CDS
TCGCATTCAGATATTTGAGA+AGG 0.574572 8.4:+1773696 None:intergenic
GTACCGCAGAGGTGGCAAGT+AGG 0.582300 8.4:+1773561 None:intergenic
CCTGAAAACAGATAAAATCA+GGG 0.584071 8.4:+1773139 None:intergenic
TTACAATGTTTGGTTTCGTG+AGG 0.584583 8.4:-1773217 MS.gene042174:CDS
GGCCTCTTCGATCGTGCAAG+AGG 0.587087 8.4:-1773458 MS.gene042174:CDS
CAATATTGACATGATTGGGA+TGG 0.597431 8.4:-1771893 MS.gene042174:CDS
CAGGACAAGGACTCTCAAGA+TGG 0.605973 8.4:+1772211 None:intergenic
TAAGACCAAATGAAGCTGGT+TGG 0.607408 8.4:-1772014 MS.gene042174:CDS
GATGTTCAGCAAGGAACGGG+TGG 0.615438 8.4:-1772169 MS.gene042174:CDS
TTCCTCTTGCACGATCGAAG+AGG 0.618164 8.4:+1773456 None:intergenic
GGGCGACAAGTCGTTTGTTG+TGG 0.622392 8.4:-1772241 MS.gene042174:CDS
GGTCAATATCCTTTACAAGA+CGG 0.623069 8.4:-1772060 MS.gene042174:CDS
GAAGCTGGAAATGGAAAGAA+CGG 0.626576 8.4:+1773530 None:intergenic
TGGTTGATCAGAATCTTCAA+GGG 0.627797 8.4:+1773422 None:intergenic
TTGGTGGAGAAAGATTTCAC+TGG 0.638514 8.4:+1772321 None:intergenic
AATGATCGCTGGGCAAAACA+GGG 0.641379 8.4:+1771803 None:intergenic
GAAGTGGCAGAAGAGGAAGA+GGG 0.647334 8.4:+1773648 None:intergenic
AGGACTCTCAAGATGGCTAG+TGG 0.647661 8.4:+1772218 None:intergenic
AATATCCTTTACAAGACGGG+TGG 0.648584 8.4:-1772056 MS.gene042174:CDS
TGTTACGTGTACCGCAGAGG+TGG 0.650688 8.4:+1773553 None:intergenic
TCTTTGTTGTATGAAACACC+AGG 0.659730 8.4:+1771862 None:intergenic
CTGAAAACAGATAAAATCAG+GGG 0.668394 8.4:+1773140 None:intergenic
ACTTTGCATTGCTGAAGTGG+AGG 0.670157 8.4:-1772268 MS.gene042174:CDS
ATGGGATCAGATGTTCAGCA+AGG 0.672446 8.4:-1772178 MS.gene042174:CDS
CGGTGTTACGTGTACCGCAG+AGG 0.683294 8.4:+1773550 None:intergenic
ATTGCTGAAGTGGAGGATGT+GGG 0.686579 8.4:-1772261 MS.gene042174:CDS
AGCCCTACTTGCCACCTCTG+CGG 0.692145 8.4:-1773564 MS.gene042174:CDS
ATTGGGATGGATGAATTACC+TGG 0.700985 8.4:-1771880 MS.gene042174:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTTTATATTTGTG+TGG - chr8.4:1772615-1772634 MS.gene042174:intron 10.0%
!!! TCAATTTTCTAAATAATTAC+CGG - chr8.4:1772509-1772528 MS.gene042174:intron 15.0%
!! TACATTACAGAATGATAAAA+AGG + chr8.4:1772796-1772815 None:intergenic 20.0%
!!! ATTTTTTAGACCGTTTTATT+TGG - chr8.4:1773099-1773118 MS.gene042174:intron 20.0%
!!! TCATAGTTTTTGTATAAGAT+TGG - chr8.4:1772901-1772920 MS.gene042174:intron 20.0%
!!! TTTTTTAGACCGTTTTATTT+GGG - chr8.4:1773100-1773119 MS.gene042174:intron 20.0%
! AAAAATACTACAAGCAAAAC+AGG + chr8.4:1773085-1773104 None:intergenic 25.0%
! ATAAGCAATATTGACATGAT+TGG - chr8.4:1773608-1773627 MS.gene042174:CDS 25.0%
! ATCATTTAGCAACTCTATAA+TGG - chr8.4:1773208-1773227 MS.gene042174:CDS 25.0%
! ATTGTTGTCAATTGTAATAC+TGG + chr8.4:1772859-1772878 None:intergenic 25.0%
! CATAGATATTAGCATAACAT+TGG + chr8.4:1773028-1773047 None:intergenic 25.0%
! TAAGCAATATTGACATGATT+GGG - chr8.4:1773609-1773628 MS.gene042174:CDS 25.0%
! TATAGAGTTGCTAAATGATT+TGG + chr8.4:1773207-1773226 None:intergenic 25.0%
! TCATTTAGCAACTCTATAAT+GGG - chr8.4:1773209-1773228 MS.gene042174:CDS 25.0%
!! TCTGTCATTTCATATATGAT+TGG - chr8.4:1772928-1772947 MS.gene042174:intron 25.0%
!!! AGATTTTGAGTTACAATGTT+TGG - chr8.4:1772279-1772298 MS.gene042174:CDS 25.0%
!!! TGTATACTTTTTCTATGTGT+CGG + chr8.4:1772434-1772453 None:intergenic 25.0%
AAAGTATACAATGTCGATGT+AGG - chr8.4:1772444-1772463 MS.gene042174:intron 30.0%
AACAAAGAGAAGAAAGTAAG+AGG - chr8.4:1773659-1773678 MS.gene042174:CDS 30.0%
AATGGTTGATCAAAATCTTC+AGG + chr8.4:1772155-1772174 None:intergenic 30.0%
ACCTGAAAACAGATAAAATC+AGG + chr8.4:1772371-1772390 None:intergenic 30.0%
ATGGTTGATCAAAATCTTCA+GGG + chr8.4:1772154-1772173 None:intergenic 30.0%
ATTTACCAGCATACAATAGT+AGG + chr8.4:1772766-1772785 None:intergenic 30.0%
CCTGAAAACAGATAAAATCA+GGG + chr8.4:1772370-1772389 None:intergenic 30.0%
CTGAAAACAGATAAAATCAG+GGG + chr8.4:1772369-1772388 None:intergenic 30.0%
TTATCTCGAAATCACGTAAA+CGG + chr8.4:1773592-1773611 None:intergenic 30.0%
! AAAAATATCAACAGCCCTTT+TGG + chr8.4:1772192-1772211 None:intergenic 30.0%
! AACGAAATCAATAGCCTTTT+TGG + chr8.4:1772126-1772145 None:intergenic 30.0%
! TAAAACGATCTAATCGCTTT+TGG + chr8.4:1773565-1773584 None:intergenic 30.0%
AAAAGCCAAGGATTCCAAAA+GGG - chr8.4:1772175-1772194 MS.gene042174:CDS 35.0%
AAAAGCCTACTATTGTATGC+TGG - chr8.4:1772758-1772777 MS.gene042174:CDS 35.0%
AAGTCAAACCAAATGTTGAC+AGG - chr8.4:1773527-1773546 MS.gene042174:CDS 35.0%
ACCCTCTTTCCCAAATAAAA+CGG + chr8.4:1773112-1773131 None:intergenic 35.0%
AGTAAGTGTTAGTATGTGTC+TGG - chr8.4:1772544-1772563 MS.gene042174:intron 35.0%
ATGGTTGATCAGAATCTTCA+AGG + chr8.4:1772088-1772107 None:intergenic 35.0%
CAATATTGACATGATTGGGA+TGG - chr8.4:1773613-1773632 MS.gene042174:CDS 35.0%
CATTCAGATATTTGAGAAGG+TGG + chr8.4:1771810-1771829 None:intergenic 35.0%
GAGAAAAGGATGAAAGCTAT+TGG - chr8.4:1772323-1772342 MS.gene042174:CDS 35.0%
GATCACTGTGACAGTATTAT+GGG - chr8.4:1773047-1773066 MS.gene042174:intron 35.0%
GGTCAATATCCTTTACAAGA+CGG - chr8.4:1773446-1773465 MS.gene042174:CDS 35.0%
GTCAATATCCTTTACAAGAC+GGG - chr8.4:1773447-1773466 MS.gene042174:CDS 35.0%
GTTGCACATACAACTATATC+AGG + chr8.4:1772824-1772843 None:intergenic 35.0%
TAAAAGCCAAGGATTCCAAA+AGG - chr8.4:1772174-1772193 MS.gene042174:CDS 35.0%
TATGAACTGGGATGACAAAA+AGG - chr8.4:1773421-1773440 MS.gene042174:CDS 35.0%
TCGCATTCAGATATTTGAGA+AGG + chr8.4:1771813-1771832 None:intergenic 35.0%
TGATCAACCATTAAAAGCCA+AGG - chr8.4:1772163-1772182 MS.gene042174:CDS 35.0%
TGATCACTGTGACAGTATTA+TGG - chr8.4:1773046-1773065 MS.gene042174:intron 35.0%
TGGTTGATCAGAATCTTCAA+GGG + chr8.4:1772087-1772106 None:intergenic 35.0%
TGGTTTCGCAAAATGTTCAT+AGG + chr8.4:1772219-1772238 None:intergenic 35.0%
! AATAGCCTTTTTGGATTCCT+TGG + chr8.4:1772117-1772136 None:intergenic 35.0%
! AGAGTTGCTAAATGATTTGG+TGG + chr8.4:1773204-1773223 None:intergenic 35.0%
! CCCTGATTTTATCTGTTTTC+AGG - chr8.4:1772367-1772386 MS.gene042174:intron 35.0%
! TCTTTGTTGTATGAAACACC+AGG + chr8.4:1773647-1773666 None:intergenic 35.0%
! TTACAATGTTTGGTTTCGTG+AGG - chr8.4:1772289-1772308 MS.gene042174:CDS 35.0%
! TTGTTCAGTTTCTTCTGAGA+AGG - chr8.4:1772728-1772747 MS.gene042174:intron 35.0%
!! ACATTTTCAAGCGATCTACA+TGG - chr8.4:1772691-1772710 MS.gene042174:intron 35.0%
!! ACTTGCTAGAAAGTGTCAAA+AGG + chr8.4:1773736-1773755 None:intergenic 35.0%
!! TGTCAACATTTGGTTTGACT+TGG + chr8.4:1773528-1773547 None:intergenic 35.0%
!! TTGGAATCCTTGGCTTTTAA+TGG + chr8.4:1772173-1772192 None:intergenic 35.0%
!!! ACCGTTTTATTTGGGAAAGA+GGG - chr8.4:1773108-1773127 MS.gene042174:intron 35.0%
AACTGGGATGACAAAAAGGA+TGG - chr8.4:1773425-1773444 MS.gene042174:CDS 40.0%
AATATCCTTTACAAGACGGG+TGG - chr8.4:1773450-1773469 MS.gene042174:CDS 40.0%
ACTTACTGTCACGTAGATAC+CGG + chr8.4:1772531-1772550 None:intergenic 40.0%
AGAACTTTGCATTGCTGAAG+TGG - chr8.4:1773235-1773254 MS.gene042174:CDS 40.0%
AGTAGCCAAGGAATCCAAAA+AGG - chr8.4:1772109-1772128 MS.gene042174:CDS 40.0%
ATCAGATGTTCAGCAAGGAA+CGG - chr8.4:1773333-1773352 MS.gene042174:CDS 40.0%
ATCGATGATGATTCTTCCGT+TGG - chr8.4:1772014-1772033 MS.gene042174:CDS 40.0%
ATTGGGATGGATGAATTACC+TGG - chr8.4:1773626-1773645 MS.gene042174:CDS 40.0%
CACCCCCAAAAACAACATTA+GGG + chr8.4:1773400-1773419 None:intergenic 40.0%
GAAGCTGGAAATGGAAAGAA+CGG + chr8.4:1771979-1771998 None:intergenic 40.0%
GATATCGTTGAAGCTGGAAA+TGG + chr8.4:1771988-1772007 None:intergenic 40.0%
GTACTAAGACCAAATGAAGC+TGG - chr8.4:1773488-1773507 MS.gene042174:CDS 40.0%
TAAGACCAAATGAAGCTGGT+TGG - chr8.4:1773492-1773511 MS.gene042174:CDS 40.0%
TACGATTGCCTGTCAACATT+TGG + chr8.4:1773538-1773557 None:intergenic 40.0%
TATGTCCAACCAGCTTCATT+TGG + chr8.4:1773500-1773519 None:intergenic 40.0%
TCACCCCCAAAAACAACATT+AGG + chr8.4:1773401-1773420 None:intergenic 40.0%
TGATCAACCATTAGTAGCCA+AGG - chr8.4:1772097-1772116 MS.gene042174:CDS 40.0%
TTGGATTCCTTGGCTACTAA+TGG + chr8.4:1772107-1772126 None:intergenic 40.0%
TTGGTGGAGAAAGATTTCAC+TGG + chr8.4:1773188-1773207 None:intergenic 40.0%
! AACATCTGATCCCATTTTGG+AGG + chr8.4:1773323-1773342 None:intergenic 40.0%
! ACATCTGATCCCATTTTGGA+GGG + chr8.4:1773322-1773341 None:intergenic 40.0%
! AGGATTTGGAGTTGGAGAAA+AGG - chr8.4:1772309-1772328 MS.gene042174:CDS 40.0%
! ATTTTGCGAAACCACAAGAG+AGG - chr8.4:1772225-1772244 MS.gene042174:CDS 40.0%
! ATTTTGTTCACCTGTGTCAC+AGG - chr8.4:1772647-1772666 MS.gene042174:intron 40.0%
! CTGAACATCTGATCCCATTT+TGG + chr8.4:1773326-1773345 None:intergenic 40.0%
! TGTTTGGTTTCGTGAGGATT+TGG - chr8.4:1772295-1772314 MS.gene042174:CDS 40.0%
! TTTTGCGAAACCACAAGAGA+GGG - chr8.4:1772226-1772245 MS.gene042174:CDS 40.0%
!! CATGATTTGCAGGACCATTT+TGG + chr8.4:1771900-1771919 None:intergenic 40.0%
!! TTTTCAAGCGATCTACATGG+TGG - chr8.4:1772694-1772713 MS.gene042174:intron 40.0%
!! TTTTGGGGGTGATATGAACT+GGG - chr8.4:1773409-1773428 MS.gene042174:CDS 40.0%
!! TTTTTGGGGGTGATATGAAC+TGG - chr8.4:1773408-1773427 MS.gene042174:CDS 40.0%
!!! AGGCACCCTAATGTTGTTTT+TGG - chr8.4:1773392-1773411 MS.gene042174:CDS 40.0%
!!! CACCCTAATGTTGTTTTTGG+GGG - chr8.4:1773395-1773414 MS.gene042174:CDS 40.0%
!!! GACCGTTTTATTTGGGAAAG+AGG - chr8.4:1773107-1773126 MS.gene042174:intron 40.0%
!!! GCACCCTAATGTTGTTTTTG+GGG - chr8.4:1773394-1773413 MS.gene042174:CDS 40.0%
!!! GGCACCCTAATGTTGTTTTT+GGG - chr8.4:1773393-1773412 MS.gene042174:CDS 40.0%
AAGAGGCGAGATCAAACAAC+TGG - chr8.4:1773676-1773695 MS.gene042174:CDS 45.0%
AAGGAGACCATAATGATCGC+TGG + chr8.4:1773717-1773736 None:intergenic 45.0%
AATGATCGCTGGGCAAAACA+GGG + chr8.4:1773706-1773725 None:intergenic 45.0%
ACTTTGCATTGCTGAAGTGG+AGG - chr8.4:1773238-1773257 MS.gene042174:CDS 45.0%
AGAAGACGAAGTCGAAGAAG+TGG + chr8.4:1771877-1771896 None:intergenic 45.0%
AGGAGACCATAATGATCGCT+GGG + chr8.4:1773716-1773735 None:intergenic 45.0%
AGGCCAGAAAACAGAACCAA+CGG + chr8.4:1772033-1772052 None:intergenic 45.0%
ATGGGATCAGATGTTCAGCA+AGG - chr8.4:1773328-1773347 MS.gene042174:CDS 45.0%
ATTGCTGAAGTGGAGGATGT+GGG - chr8.4:1773245-1773264 MS.gene042174:CDS 45.0%
CGACTTCGTCTTCTCCAAAA+TGG - chr8.4:1771883-1771902 MS.gene042174:CDS 45.0%
GTCGTTGATATCGTTGAAGC+TGG + chr8.4:1771994-1772013 None:intergenic 45.0%
TAATGATCGCTGGGCAAAAC+AGG + chr8.4:1773707-1773726 None:intergenic 45.0%
TCAGATGTTCAGCAAGGAAC+GGG - chr8.4:1773334-1773353 MS.gene042174:CDS 45.0%
! CACAAGAGAGGGTTGATTCT+GGG - chr8.4:1772237-1772256 MS.gene042174:CDS 45.0%
! GAAGAGTGTGCATGATTTGC+AGG + chr8.4:1771910-1771929 None:intergenic 45.0%
! GTTTCGTGAGGATTTGGAGT+TGG - chr8.4:1772301-1772320 MS.gene042174:CDS 45.0%
! GTTTTGCCCAGCGATCATTA+TGG - chr8.4:1773707-1773726 MS.gene042174:CDS 45.0%
!! AGGCTCTGAACATCCTCAAA+AGG - chr8.4:1773372-1773391 MS.gene042174:CDS 45.0%
!! ATCAAGCACTGACGCAGAAT+CGG + chr8.4:1772573-1772592 None:intergenic 45.0%
!! CAACATTAGGGTGCCTTTTG+AGG + chr8.4:1773388-1773407 None:intergenic 45.0%
!! CATCTGATCCCATTTTGGAG+GGG + chr8.4:1773321-1773340 None:intergenic 45.0%
!!! AACAGCCCTTTTGGAATCCT+TGG + chr8.4:1772183-1772202 None:intergenic 45.0%
!!! CTTCCGTTGGTTCTGTTTTC+TGG - chr8.4:1772027-1772046 MS.gene042174:CDS 45.0%
AAGTGGCAGAAGAGGAAGAG+GGG + chr8.4:1771860-1771879 None:intergenic 50.0%
AGAAGTGGCAGAAGAGGAAG+AGG + chr8.4:1771862-1771881 None:intergenic 50.0%
AGGACTCTCAAGATGGCTAG+TGG + chr8.4:1773291-1773310 None:intergenic 50.0%
AGTCGAAGAAGTGGCAGAAG+AGG + chr8.4:1771868-1771887 None:intergenic 50.0%
CAGGACAAGGACTCTCAAGA+TGG + chr8.4:1773298-1773317 None:intergenic 50.0%
CATTGCTGAAGTGGAGGATG+TGG - chr8.4:1773244-1773263 MS.gene042174:CDS 50.0%
CCAGAATCAACCCTCTCTTG+TGG + chr8.4:1772239-1772258 None:intergenic 50.0%
GAAGTGGCAGAAGAGGAAGA+GGG + chr8.4:1771861-1771880 None:intergenic 50.0%
TCAAGCCACCCGTCTTGTAA+AGG + chr8.4:1773458-1773477 None:intergenic 50.0%
TTCCTCTTGCACGATCGAAG+AGG + chr8.4:1772053-1772072 None:intergenic 50.0%
TTGTCCTGCCCCTCCAAAAT+GGG - chr8.4:1773310-1773329 MS.gene042174:CDS 50.0%
TTGTTCACCTGTGTCACAGG+AGG - chr8.4:1772650-1772669 MS.gene042174:intron 50.0%
! CCACAAGAGAGGGTTGATTC+TGG - chr8.4:1772236-1772255 MS.gene042174:CDS 50.0%
! GAGAGGGTTGATTCTGGGAA+AGG - chr8.4:1772242-1772261 MS.gene042174:CDS 50.0%
AAGACGGGTGGCTTGATGCT+TGG - chr8.4:1773462-1773481 MS.gene042174:CDS 55.0%
CGAGTAACCTCCTGTGACAC+AGG + chr8.4:1772660-1772679 None:intergenic 55.0%
CTTGTCCTGCCCCTCCAAAA+TGG - chr8.4:1773309-1773328 MS.gene042174:CDS 55.0%
GATGTTCAGCAAGGAACGGG+TGG - chr8.4:1773337-1773356 MS.gene042174:CDS 55.0%
TACCGCAGAGGTGGCAAGTA+GGG + chr8.4:1771947-1771966 None:intergenic 55.0%
TGTTACGTGTACCGCAGAGG+TGG + chr8.4:1771956-1771975 None:intergenic 55.0%
! GGGCGACAAGTCGTTTGTTG+TGG - chr8.4:1773265-1773284 MS.gene042174:CDS 55.0%
!! CATTTTGGAGGGGCAGGACA+AGG + chr8.4:1773311-1773330 None:intergenic 55.0%
!! TGATCCCATTTTGGAGGGGC+AGG + chr8.4:1773317-1773336 None:intergenic 55.0%
ACGGGTGGAGCAGGCTAATG+AGG - chr8.4:1773352-1773371 MS.gene042174:CDS 60.0%
AGCCCTACTTGCCACCTCTG+CGG - chr8.4:1771942-1771961 MS.gene042174:CDS 60.0%
GCAGAAGAGGAAGAGGGGTG+TGG + chr8.4:1771855-1771874 None:intergenic 60.0%
GGCCTCTTCGATCGTGCAAG+AGG - chr8.4:1772048-1772067 MS.gene042174:CDS 60.0%
GTACCGCAGAGGTGGCAAGT+AGG + chr8.4:1771948-1771967 None:intergenic 60.0%
! GAAGAGGAAGAGGGGTGTGG+AGG + chr8.4:1771852-1771871 None:intergenic 60.0%
!! CGGTGTTACGTGTACCGCAG+AGG + chr8.4:1771959-1771978 None:intergenic 60.0%
CAGCAAGGAACGGGTGGAGC+AGG - chr8.4:1773343-1773362 MS.gene042174:CDS 65.0%
! GGAAGAGGGGTGTGGAGGTG+AGG + chr8.4:1771847-1771866 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 1771771 1773757 1771771 ID=MS.gene042174
chr8.4 mRNA 1771771 1773757 1771771 ID=MS.gene042174.t1;Parent=MS.gene042174
chr8.4 exon 1773140 1773757 1773140 ID=MS.gene042174.t1.exon1;Parent=MS.gene042174.t1
chr8.4 CDS 1773140 1773757 1773140 ID=cds.MS.gene042174.t1;Parent=MS.gene042174.t1
chr8.4 exon 1772749 1772859 1772749 ID=MS.gene042174.t1.exon2;Parent=MS.gene042174.t1
chr8.4 CDS 1772749 1772859 1772749 ID=cds.MS.gene042174.t1;Parent=MS.gene042174.t1
chr8.4 exon 1771771 1772355 1771771 ID=MS.gene042174.t1.exon3;Parent=MS.gene042174.t1
chr8.4 CDS 1771771 1772355 1771771 ID=cds.MS.gene042174.t1;Parent=MS.gene042174.t1
Gene Sequence

>MS.gene042174

ATGTCTTGGTCATGCAAAAAATGCACTTTCGTCAATCCACCTTCTCAAATATCTGAATGCGAAATTTGTTTCTCCTCACCTCCACACCCCTCTTCCTCTTCTGCCACTTCTTCGACTTCGTCTTCTCCAAAATGGTCCTGCAAATCATGCACACTCTTCAACTCTTACAAAAGCCCTACTTGCCACCTCTGCGGTACACGTAACACCGTTCTTTCCATTTCCAGCTTCAACGATATCAACGACATCGATGATGATTCTTCCGTTGGTTCTGTTTTCTGGCCTCTTCGATCGTGCAAGAGGAAAGCTGTAGATTCCCTTGAAGATTCTGATCAACCATTAGTAGCCAAGGAATCCAAAAAGGCTATTGATTTCGTTGATTTCCCTGAAGATTTTGATCAACCATTAAAAGCCAAGGATTCCAAAAGGGCTGTTGATATTTTTGATTCCTATGAACATTTTGCGAAACCACAAGAGAGGGTTGATTCTGGGAAAGGTGTTAGTTCGTTGAAGATTTTGAGTTACAATGTTTGGTTTCGTGAGGATTTGGAGTTGGAGAAAAGGATGAAAGCTATTGGTGATCTTGTTTTGTTGCATTCCCCTGATTTTATCTGTTTTCAGGTTTCATTATCATTGCATATTATATTTACTATCTTTGTAGCACCGACACATAGAAAAAGTATACAATGTCGATGTAGGACACTAACACGATACTGACACATGTGATTACATTTAATTACGTCAATTTTCTAAATAATTACCGGTATCTACGTGACAGTAAGTGTTAGTATGTGTCTGGTGTCCGATTCTGCGTCAGTGCTTGATAGTTTACAATACACTTTTTTTATTTTTTTTTTTATATTTGTGTGGCTTTGTTTGATTTTGTTCACCTGTGTCACAGGAGGTTACTCGCGATATATATGACATTTTCAAGCGATCTACATGGTGGAATGTATATCATTGTTCAGTTTCTTCTGAGAAGGCTTATTCAAAAGCCTACTATTGTATGCTGGTAAATTTCACAACCTTTTTATCATTCTGTAATGTAATCTGCCTGATATAGTTGTATGTGCAACAATCTTTATAATCCAGTATTACAATTGACAACAATGACACATTTGTTTTGATTATTATCATAGTTTTTGTATAAGATTGGTACATCTGTCATTTCATATATGATTGGTACATCTGTTATATTGTCTGCTAGCATGAAATCTGTTCAGAAGAGCATCAAGCTTCGTGCTAAATAACTGTATGCCAATGTTATGCTAATATCTATGATCACTGTGACAGTATTATGGGTAAAAAGCTCATCCTGTTTTGCTTGTAGTATTTTTTAGACCGTTTTATTTGGGAAAGAGGGTGTTATTCTTATGTACTGAAATATCTGTCAAATTAATTTCAGTTAAGCAAACTGCCAGTGAAATCTTTCTCCACCAAATCATTTAGCAACTCTATAATGGGAAGAGAACTTTGCATTGCTGAAGTGGAGGATGTGGGCGACAAGTCGTTTGTTGTGGCCACTAGCCATCTTGAGAGTCCTTGTCCTGCCCCTCCAAAATGGGATCAGATGTTCAGCAAGGAACGGGTGGAGCAGGCTAATGAGGCTCTGAACATCCTCAAAAGGCACCCTAATGTTGTTTTTGGGGGTGATATGAACTGGGATGACAAAAAGGATGGTCAATATCCTTTACAAGACGGGTGGCTTGATGCTTGGTCTGTACTAAGACCAAATGAAGCTGGTTGGACATATGATACCAAGTCAAACCAAATGTTGACAGGCAATCGTACTCTCCAAAAGCGATTAGATCGTTTTATTTGCCGTTTACGTGATTTCGAGATAAGCAATATTGACATGATTGGGATGGATGAATTACCTGGTGTTTCATACAACAAAGAGAAGAAAGTAAGAGGCGAGATCAAACAACTGGTATGCCCTGTTTTGCCCAGCGATCATTATGGTCTCCTTTTGACACTTTCTAGCAAGTAG
Protein sequence

>MS.gene042174.t1

MSWSCKKCTFVNPPSQISECEICFSSPPHPSSSSATSSTSSSPKWSCKSCTLFNSYKSPTCHLCGTRNTVLSISSFNDINDIDDDSSVGSVFWPLRSCKRKAVDSLEDSDQPLVAKESKKAIDFVDFPEDFDQPLKAKDSKRAVDIFDSYEHFAKPQERVDSGKGVSSLKILSYNVWFREDLELEKRMKAIGDLVLLHSPDFICFQEVTRDIYDIFKRSTWWNVYHCSVSSEKAYSKAYYCMLLSKLPVKSFSTKSFSNSIMGRELCIAEVEDVGDKSFVVATSHLESPCPAPPKWDQMFSKERVEQANEALNILKRHPNVVFGGDMNWDDKKDGQYPLQDGWLDAWSVLRPNEAGWTYDTKSNQMLTGNRTLQKRLDRFICRLRDFEISNIDMIGMDELPGVSYNKEKKVRGEIKQLVCPVLPSDHYGLLLTLSSK