Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71597.t1 | XP_013469961.1 | 91.8 | 463 | 23 | 3 | 1 | 449 | 1 | 462 | 9.30E-236 | 825.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71597.t1 | Q9ZUB8 | 35.4 | 491 | 253 | 17 | 1 | 446 | 1 | 472 | 2.4e-59 | 231.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71597.t1 | I3T224 | 91.8 | 463 | 23 | 3 | 1 | 449 | 1 | 462 | 6.7e-236 | 825.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene71597 | 0.804122 | 2.51E-49 | -1.69E-46 |
| MS.gene049254 | MS.gene71597 | 0.859358 | 4.37E-63 | -1.69E-46 |
| MS.gene049283 | MS.gene71597 | 0.863486 | 2.41E-64 | -1.69E-46 |
| MS.gene049404 | MS.gene71597 | 0.810287 | 1.24E-50 | -1.69E-46 |
| MS.gene049424 | MS.gene71597 | 0.802693 | 4.97E-49 | -1.69E-46 |
| MS.gene049452 | MS.gene71597 | 0.821987 | 3.01E-53 | -1.69E-46 |
| MS.gene049499 | MS.gene71597 | 0.813977 | 1.95E-51 | -1.69E-46 |
| MS.gene049983 | MS.gene71597 | 0.857484 | 1.58E-62 | -1.69E-46 |
| MS.gene050073 | MS.gene71597 | 0.815289 | 9.98E-52 | -1.69E-46 |
| MS.gene050105 | MS.gene71597 | 0.812049 | 5.16E-51 | -1.69E-46 |
| MS.gene050273 | MS.gene71597 | 0.805366 | 1.38E-49 | -1.69E-46 |
| MS.gene050414 | MS.gene71597 | 0.807478 | 4.96E-50 | -1.69E-46 |
| MS.gene050559 | MS.gene71597 | 0.832301 | 1.02E-55 | -1.69E-46 |
| MS.gene050561 | MS.gene71597 | 0.83284 | 7.51E-56 | -1.69E-46 |
| MS.gene050562 | MS.gene71597 | 0.826209 | 3.08E-54 | -1.69E-46 |
| MS.gene050571 | MS.gene71597 | 0.808817 | 2.57E-50 | -1.69E-46 |
| MS.gene050646 | MS.gene71597 | 0.813105 | 3.03E-51 | -1.69E-46 |
| MS.gene050654 | MS.gene71597 | 0.81411 | 1.82E-51 | -1.69E-46 |
| MS.gene050759 | MS.gene71597 | 0.832801 | 7.68E-56 | -1.69E-46 |
| MS.gene050962 | MS.gene71597 | 0.819016 | 1.45E-52 | -1.69E-46 |
| MS.gene051198 | MS.gene71597 | 0.826427 | 2.73E-54 | -1.69E-46 |
| MS.gene051279 | MS.gene71597 | 0.814576 | 1.44E-51 | -1.69E-46 |
| MS.gene051332 | MS.gene71597 | 0.805529 | 1.28E-49 | -1.69E-46 |
| MS.gene051368 | MS.gene71597 | 0.837407 | 5.29E-57 | -1.69E-46 |
| MS.gene051543 | MS.gene71597 | 0.828734 | 7.63E-55 | -1.69E-46 |
| MS.gene051597 | MS.gene71597 | 0.837742 | 4.34E-57 | -1.69E-46 |
| MS.gene051744 | MS.gene71597 | 0.812197 | 4.78E-51 | -1.69E-46 |
| MS.gene052037 | MS.gene71597 | 0.817071 | 3.99E-52 | -1.69E-46 |
| MS.gene052108 | MS.gene71597 | 0.815563 | 8.67E-52 | -1.69E-46 |
| MS.gene05216 | MS.gene71597 | 0.803034 | 4.23E-49 | -1.69E-46 |
| MS.gene052239 | MS.gene71597 | -0.801096 | 1.06E-48 | -1.69E-46 |
| MS.gene052463 | MS.gene71597 | 0.814147 | 1.79E-51 | -1.69E-46 |
| MS.gene052500 | MS.gene71597 | 0.831109 | 2.01E-55 | -1.69E-46 |
| MS.gene052502 | MS.gene71597 | 0.829174 | 5.96E-55 | -1.69E-46 |
| MS.gene052534 | MS.gene71597 | 0.807594 | 4.68E-50 | -1.69E-46 |
| MS.gene052582 | MS.gene71597 | 0.821743 | 3.43E-53 | -1.69E-46 |
| MS.gene052584 | MS.gene71597 | 0.821738 | 3.44E-53 | -1.69E-46 |
| MS.gene052674 | MS.gene71597 | 0.819508 | 1.12E-52 | -1.69E-46 |
| MS.gene052675 | MS.gene71597 | 0.821457 | 4.00E-53 | -1.69E-46 |
| MS.gene052677 | MS.gene71597 | 0.823326 | 1.47E-53 | -1.69E-46 |
| MS.gene052826 | MS.gene71597 | 0.830673 | 2.57E-55 | -1.69E-46 |
| MS.gene052954 | MS.gene71597 | 0.829821 | 4.15E-55 | -1.69E-46 |
| MS.gene053006 | MS.gene71597 | 0.814512 | 1.48E-51 | -1.69E-46 |
| MS.gene053079 | MS.gene71597 | 0.821584 | 3.74E-53 | -1.69E-46 |
| MS.gene05308 | MS.gene71597 | 0.813895 | 2.03E-51 | -1.69E-46 |
| MS.gene053226 | MS.gene71597 | 0.831628 | 1.50E-55 | -1.69E-46 |
| MS.gene053931 | MS.gene71597 | 0.825351 | 4.92E-54 | -1.69E-46 |
| MS.gene053963 | MS.gene71597 | 0.827954 | 1.18E-54 | -1.69E-46 |
| MS.gene05415 | MS.gene71597 | 0.822796 | 1.96E-53 | -1.69E-46 |
| MS.gene054236 | MS.gene71597 | 0.805604 | 1.23E-49 | -1.69E-46 |
| MS.gene054479 | MS.gene71597 | 0.850889 | 1.27E-60 | -1.69E-46 |
| MS.gene054576 | MS.gene71597 | 0.823381 | 1.43E-53 | -1.69E-46 |
| MS.gene054651 | MS.gene71597 | 0.834295 | 3.26E-56 | -1.69E-46 |
| MS.gene054892 | MS.gene71597 | 0.845145 | 4.88E-59 | -1.69E-46 |
| MS.gene054893 | MS.gene71597 | 0.845322 | 4.36E-59 | -1.69E-46 |
| MS.gene054957 | MS.gene71597 | 0.818357 | 2.04E-52 | -1.69E-46 |
| MS.gene05517 | MS.gene71597 | 0.802408 | 5.69E-49 | -1.69E-46 |
| MS.gene055222 | MS.gene71597 | 0.816168 | 6.36E-52 | -1.69E-46 |
| MS.gene055228 | MS.gene71597 | 0.801307 | 9.58E-49 | -1.69E-46 |
| MS.gene055513 | MS.gene71597 | 0.806557 | 7.76E-50 | -1.69E-46 |
| MS.gene055724 | MS.gene71597 | 0.837227 | 5.89E-57 | -1.69E-46 |
| MS.gene055841 | MS.gene71597 | 0.817446 | 3.28E-52 | -1.69E-46 |
| MS.gene056045 | MS.gene71597 | 0.816658 | 4.94E-52 | -1.69E-46 |
| MS.gene056174 | MS.gene71597 | 0.82072 | 5.91E-53 | -1.69E-46 |
| MS.gene056480 | MS.gene71597 | 0.820041 | 8.46E-53 | -1.69E-46 |
| MS.gene056506 | MS.gene71597 | 0.807605 | 4.66E-50 | -1.69E-46 |
| MS.gene05659 | MS.gene71597 | 0.802249 | 6.14E-49 | -1.69E-46 |
| MS.gene056699 | MS.gene71597 | 0.823014 | 1.74E-53 | -1.69E-46 |
| MS.gene056776 | MS.gene71597 | 0.83476 | 2.49E-56 | -1.69E-46 |
| MS.gene056779 | MS.gene71597 | 0.81591 | 7.26E-52 | -1.69E-46 |
| MS.gene056834 | MS.gene71597 | 0.805077 | 1.59E-49 | -1.69E-46 |
| MS.gene05686 | MS.gene71597 | 0.835273 | 1.85E-56 | -1.69E-46 |
| MS.gene05690 | MS.gene71597 | 0.812098 | 5.03E-51 | -1.69E-46 |
| MS.gene056973 | MS.gene71597 | 0.850587 | 1.54E-60 | -1.69E-46 |
| MS.gene057034 | MS.gene71597 | 0.842402 | 2.64E-58 | -1.69E-46 |
| MS.gene057102 | MS.gene71597 | 0.806057 | 9.89E-50 | -1.69E-46 |
| MS.gene057155 | MS.gene71597 | 0.832271 | 1.04E-55 | -1.69E-46 |
| MS.gene057208 | MS.gene71597 | 0.870385 | 1.52E-66 | -1.69E-46 |
| MS.gene057371 | MS.gene71597 | 0.807256 | 5.52E-50 | -1.69E-46 |
| MS.gene058008 | MS.gene71597 | 0.83235 | 9.94E-56 | -1.69E-46 |
| MS.gene058009 | MS.gene71597 | 0.824299 | 8.70E-54 | -1.69E-46 |
| MS.gene058010 | MS.gene71597 | 0.835365 | 1.75E-56 | -1.69E-46 |
| MS.gene058159 | MS.gene71597 | 0.800659 | 1.30E-48 | -1.69E-46 |
| MS.gene058223 | MS.gene71597 | -0.816202 | 6.24E-52 | -1.69E-46 |
| MS.gene058279 | MS.gene71597 | 0.83752 | 4.95E-57 | -1.69E-46 |
| MS.gene058427 | MS.gene71597 | 0.812553 | 4.00E-51 | -1.69E-46 |
| MS.gene058548 | MS.gene71597 | 0.808231 | 3.43E-50 | -1.69E-46 |
| MS.gene058781 | MS.gene71597 | 0.841193 | 5.51E-58 | -1.69E-46 |
| MS.gene058783 | MS.gene71597 | 0.843245 | 1.58E-58 | -1.69E-46 |
| MS.gene058979 | MS.gene71597 | 0.84354 | 1.32E-58 | -1.69E-46 |
| MS.gene05952 | MS.gene71597 | 0.828637 | 8.05E-55 | -1.69E-46 |
| MS.gene05985 | MS.gene71597 | -0.813308 | 2.73E-51 | -1.69E-46 |
| MS.gene059965 | MS.gene71597 | 0.808337 | 3.25E-50 | -1.69E-46 |
| MS.gene060011 | MS.gene71597 | 0.819824 | 9.48E-53 | -1.69E-46 |
| MS.gene060244 | MS.gene71597 | 0.802067 | 6.69E-49 | -1.69E-46 |
| MS.gene060310 | MS.gene71597 | 0.813036 | 3.14E-51 | -1.69E-46 |
| MS.gene060365 | MS.gene71597 | 0.821678 | 3.55E-53 | -1.69E-46 |
| MS.gene060412 | MS.gene71597 | 0.803258 | 3.80E-49 | -1.69E-46 |
| MS.gene060520 | MS.gene71597 | 0.812924 | 3.32E-51 | -1.69E-46 |
| MS.gene060527 | MS.gene71597 | 0.810189 | 1.30E-50 | -1.69E-46 |
| MS.gene06052 | MS.gene71597 | 0.819332 | 1.23E-52 | -1.69E-46 |
| MS.gene060840 | MS.gene71597 | 0.835679 | 1.46E-56 | -1.69E-46 |
| MS.gene060932 | MS.gene71597 | 0.827904 | 1.21E-54 | -1.69E-46 |
| MS.gene060933 | MS.gene71597 | 0.814821 | 1.27E-51 | -1.69E-46 |
| MS.gene060935 | MS.gene71597 | 0.840782 | 7.07E-58 | -1.69E-46 |
| MS.gene060937 | MS.gene71597 | 0.840752 | 7.20E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71597.t1 | MTR_1g106020 | 93.737 | 463 | 14 | 3 | 1 | 449 | 1 | 462 | 0.0 | 874 |
| MS.gene71597.t1 | MTR_4g019560 | 75.655 | 267 | 60 | 4 | 141 | 405 | 5 | 268 | 1.72e-139 | 400 |
| MS.gene71597.t1 | MTR_7g057330 | 76.866 | 268 | 56 | 5 | 141 | 405 | 5 | 269 | 5.16e-132 | 382 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71597.t1 | AT1G23780 | 36.162 | 495 | 244 | 16 | 1 | 446 | 1 | 472 | 3.75e-65 | 217 |
| MS.gene71597.t1 | AT1G23770 | 40.753 | 292 | 136 | 11 | 140 | 411 | 45 | 319 | 1.09e-44 | 159 |
| MS.gene71597.t1 | AT1G70360 | 37.963 | 216 | 103 | 6 | 134 | 345 | 5 | 193 | 3.90e-32 | 121 |
Find 107 sgRNAs with CRISPR-Local
Find 131 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGGAAAATTCTGAATTTAA+TGG | 0.156965 | 1.4:-81975138 | MS.gene71597:CDS |
| TCGTCAATTGGGGTGATTTC+CGG | 0.218916 | 1.4:+81975633 | None:intergenic |
| TACCAGACCGACGAGGATTT+TGG | 0.225156 | 1.4:+81974758 | None:intergenic |
| AAGAAGTTTGAGGAGGAGTT+TGG | 0.230453 | 1.4:-81974871 | MS.gene71597:CDS |
| ATCTCTGTGTCATTCACTTC+TGG | 0.233231 | 1.4:+81975588 | None:intergenic |
| AATTTGAAAATTCAGACTTT+AGG | 0.238179 | 1.4:-81975264 | MS.gene71597:CDS |
| GGGAGATTTACACCGAAATT+TGG | 0.246277 | 1.4:+81974655 | None:intergenic |
| AGAAGATTTCCTAATCGTTT+TGG | 0.261143 | 1.4:-81974715 | MS.gene71597:CDS |
| ATTTGAAAATTCAGACTTTA+GGG | 0.262398 | 1.4:-81975263 | MS.gene71597:CDS |
| CATGCTGTTATTCTTGAATC+AGG | 0.284345 | 1.4:-81975426 | MS.gene71597:CDS |
| ATAACCCTCTTCACGAAATC+AGG | 0.302921 | 1.4:+81975504 | None:intergenic |
| TTTCGTGAAGAGGGTTATTA+AGG | 0.319053 | 1.4:-81975499 | MS.gene71597:CDS |
| TGTTCGTGTGGACAAGGATT+CGG | 0.325891 | 1.4:-81975400 | MS.gene71597:CDS |
| TCTCTGTGTCATTCACTTCT+GGG | 0.335269 | 1.4:+81975589 | None:intergenic |
| CTGATATTTGAGTATCTTCC+CGG | 0.343546 | 1.4:-81974967 | MS.gene71597:CDS |
| CTCTTCACGAAATCAGGTTC+TGG | 0.346389 | 1.4:+81975510 | None:intergenic |
| CTTCCTGAGATTCTTACTAA+TGG | 0.347157 | 1.4:-81975303 | MS.gene71597:CDS |
| GTTCGTGTGGACAAGGATTC+GGG | 0.348135 | 1.4:-81975399 | MS.gene71597:CDS |
| TCATACTCTCCACCCCAAAT+TGG | 0.358790 | 1.4:+81974685 | None:intergenic |
| TTGAGTGGAGGGAATCGTTT+GGG | 0.361185 | 1.4:+81975754 | None:intergenic |
| GTGAGGGAGGGTTTCGTTGT+GGG | 0.364950 | 1.4:+81975677 | None:intergenic |
| CTTCATCGATGATATCTTTG+AGG | 0.373258 | 1.4:-81975332 | MS.gene71597:CDS |
| GGGTTTATTTAGATAAATCT+AGG | 0.373790 | 1.4:-81975185 | MS.gene71597:CDS |
| GGAGGCATTCCAAAACGATT+AGG | 0.376005 | 1.4:+81974706 | None:intergenic |
| CTTCCACAAGTCATCATTTG+AGG | 0.389433 | 1.4:+81974894 | None:intergenic |
| TATGATCTGCAGCCAAATTT+CGG | 0.392195 | 1.4:-81974667 | MS.gene71597:CDS |
| TCGTTGTGGGAGAAAGCGTT+AGG | 0.398566 | 1.4:+81975690 | None:intergenic |
| GGATTATTCTTTACAGCCAT+TGG | 0.402179 | 1.4:+81975531 | None:intergenic |
| AGAACATGTGCAACAAACTT+TGG | 0.424994 | 1.4:+81974933 | None:intergenic |
| TTCCAAAATCCTCGTCGGTC+TGG | 0.425234 | 1.4:-81974760 | MS.gene71597:CDS |
| GTACGCCTTGGTGCGTATGC+AGG | 0.431964 | 1.4:+81974634 | None:intergenic |
| ATTGATCTTTGTGATAAAGC+TGG | 0.436023 | 1.4:-81975039 | MS.gene71597:CDS |
| AGATCTGAGTCTAAGCTTCA+TGG | 0.437460 | 1.4:+81975926 | None:intergenic |
| TCACAAAGATCAATTAATAG+TGG | 0.442243 | 1.4:+81975048 | None:intergenic |
| TTGAGGAGGAGTTTGGACAA+AGG | 0.443922 | 1.4:-81974864 | MS.gene71597:CDS |
| GAATGATGGGTTTGTGAGGG+AGG | 0.444874 | 1.4:+81975664 | None:intergenic |
| GAACTTGTTTGCTCAGTATC+GGG | 0.445839 | 1.4:-81974815 | MS.gene71597:CDS |
| TTTGAAGCTGTTGGTGTTTG+CGG | 0.446063 | 1.4:-81975451 | MS.gene71597:CDS |
| TGTTGAAAGGAACATGAATC+AGG | 0.448793 | 1.4:+81975870 | None:intergenic |
| AGAACTTGTTTGCTCAGTAT+CGG | 0.450357 | 1.4:-81974816 | MS.gene71597:CDS |
| CACTCAATCGGCATCGCCGC+AGG | 0.453536 | 1.4:-81975738 | MS.gene71597:CDS |
| TGTGAGGGAGGGTTTCGTTG+TGG | 0.461233 | 1.4:+81975676 | None:intergenic |
| AATGATGGGTTTGTGAGGGA+GGG | 0.462658 | 1.4:+81975665 | None:intergenic |
| ATTGAGTGGAGGGAATCGTT+TGG | 0.462925 | 1.4:+81975753 | None:intergenic |
| GAACCATTAGTAAGAATCTC+AGG | 0.464510 | 1.4:+81975300 | None:intergenic |
| AGAGGAAGAAGATGAAGGGA+TGG | 0.470385 | 1.4:+81975824 | None:intergenic |
| AGGAGGAGTTTGGACAAAGG+AGG | 0.475693 | 1.4:-81974861 | MS.gene71597:CDS |
| AACGATTCCCTCCACTCAAT+CGG | 0.477334 | 1.4:-81975750 | MS.gene71597:CDS |
| ATCAGCAGCTTAAGCTCCAT+TGG | 0.477822 | 1.4:+81974985 | None:intergenic |
| GGAGGAGTTTGGACAAAGGA+GGG | 0.490390 | 1.4:-81974860 | MS.gene71597:CDS |
| TGTGGAAGATAGTGAAAGAT+GGG | 0.491598 | 1.4:-81975080 | MS.gene71597:CDS |
| ATGCTGGTTCGAGGGTGCAT+AGG | 0.499836 | 1.4:-81975206 | MS.gene71597:CDS |
| TGCTGGTTCGAGGGTGCATA+GGG | 0.501242 | 1.4:-81975205 | MS.gene71597:CDS |
| AACTTTGGCTAAATCATCAC+CGG | 0.504693 | 1.4:+81974948 | None:intergenic |
| GTATTATGAGGTTCTTTCAA+AGG | 0.519091 | 1.4:-81974738 | MS.gene71597:CDS |
| TACTCTCCACCCCAAATTGG+AGG | 0.519196 | 1.4:+81974688 | None:intergenic |
| CAGAACCTGATTTCGTGAAG+AGG | 0.519233 | 1.4:-81975509 | MS.gene71597:CDS |
| CGTTGTGGGAGAAAGCGTTA+GGG | 0.523226 | 1.4:+81975691 | None:intergenic |
| TGTGGACAAGGATTCGGGTA+TGG | 0.525822 | 1.4:-81975394 | MS.gene71597:CDS |
| ATAGAAGAGGAAGAAGATGA+AGG | 0.527216 | 1.4:+81975819 | None:intergenic |
| TAGAAGAGGAAGAAGATGAA+GGG | 0.527923 | 1.4:+81975820 | None:intergenic |
| TACGCCTTGGTGCGTATGCA+GGG | 0.531619 | 1.4:+81974635 | None:intergenic |
| GGTTCATTATGTGATGATGC+TGG | 0.532051 | 1.4:-81975222 | MS.gene71597:CDS |
| AATGACACAGAGATGGTTGA+TGG | 0.532293 | 1.4:-81975579 | MS.gene71597:CDS |
| GAGGGAGAGATGAATAGAAG+AGG | 0.533876 | 1.4:+81975806 | None:intergenic |
| GGCGATGCCGATTGAGTGGA+GGG | 0.535146 | 1.4:+81975743 | None:intergenic |
| CTCTGTGTCATTCACTTCTG+GGG | 0.536525 | 1.4:+81975590 | None:intergenic |
| GGTTGAAAGTGTAGAAGATG+AGG | 0.541791 | 1.4:+81975712 | None:intergenic |
| TGTGAATTTGAGTTGTTGAA+AGG | 0.551323 | 1.4:+81975857 | None:intergenic |
| TGATGGGTCTGATGAGGCGG+CGG | 0.552750 | 1.4:-81975562 | MS.gene71597:CDS |
| TTGTGGAAGATAGTGAAAGA+TGG | 0.556962 | 1.4:-81975081 | MS.gene71597:CDS |
| TAAGCCTTTAGAATTGATGC+TGG | 0.560988 | 1.4:-81975157 | MS.gene71597:CDS |
| TGTTAGTGATTTGAAGCTGT+TGG | 0.562975 | 1.4:-81975460 | MS.gene71597:CDS |
| ACTTCTGGGGTGTTCAAAGT+CGG | 0.564720 | 1.4:+81975603 | None:intergenic |
| CTCGTCGGTCTGGTATTATG+AGG | 0.566438 | 1.4:-81974750 | MS.gene71597:CDS |
| AGAACCTGATTTCGTGAAGA+GGG | 0.578359 | 1.4:-81975508 | MS.gene71597:CDS |
| GTGGAAGAAGAAGTTTGAGG+AGG | 0.585743 | 1.4:-81974878 | MS.gene71597:CDS |
| TTTGGAATGCCTCCAATTTG+GGG | 0.586726 | 1.4:-81974697 | MS.gene71597:CDS |
| ACAAGTCATCATTTGAGGCA+AGG | 0.591377 | 1.4:+81974899 | None:intergenic |
| ACCCATCATTCAAAACTCAC+CGG | 0.591595 | 1.4:-81975652 | MS.gene71597:CDS |
| CTTGTGGAAGAAGAAGTTTG+AGG | 0.592771 | 1.4:-81974881 | MS.gene71597:CDS |
| CGGCGATGCCGATTGAGTGG+AGG | 0.593068 | 1.4:+81975742 | None:intergenic |
| GGAATGCCTCCAATTTGGGG+TGG | 0.598775 | 1.4:-81974694 | MS.gene71597:CDS |
| ATTCTTTACAGCCATTGGCA+TGG | 0.600901 | 1.4:+81975536 | None:intergenic |
| TTTGAATGATGGGTTTGTGA+GGG | 0.603525 | 1.4:+81975661 | None:intergenic |
| TTGCCTCAAATGATGACTTG+TGG | 0.605171 | 1.4:-81974897 | MS.gene71597:CDS |
| GGTTGATGGGTCTGATGAGG+CGG | 0.608000 | 1.4:-81975565 | MS.gene71597:CDS |
| AGGGTTATTAAGGAAGCGCT+CGG | 0.613101 | 1.4:-81975489 | MS.gene71597:CDS |
| CTGCGGCGATGCCGATTGAG+TGG | 0.613534 | 1.4:+81975739 | None:intergenic |
| GAAATTCTCCTAGATGACAC+GGG | 0.615776 | 1.4:+81974602 | None:intergenic |
| TATGTGATGATGCTGGTTCG+AGG | 0.616388 | 1.4:-81975215 | MS.gene71597:CDS |
| TGAAATTCTCCTAGATGACA+CGG | 0.619194 | 1.4:+81974601 | None:intergenic |
| ATGTGATGATGCTGGTTCGA+GGG | 0.624487 | 1.4:-81975214 | MS.gene71597:CDS |
| ATCTCCCTGCATACGCACCA+AGG | 0.628874 | 1.4:-81974639 | MS.gene71597:CDS |
| AGAAGTGAATGACACAGAGA+TGG | 0.634353 | 1.4:-81975586 | MS.gene71597:CDS |
| GGCGGCGGTGACCATGCCAA+TGG | 0.637979 | 1.4:-81975547 | MS.gene71597:CDS |
| GTGGAGGGAATCGTTTGGGG+AGG | 0.649816 | 1.4:+81975758 | None:intergenic |
| ATTGGAAGCCGCATAAAACA+CGG | 0.660713 | 1.4:+81975003 | None:intergenic |
| ATGACACAGAGATGGTTGAT+GGG | 0.663590 | 1.4:-81975578 | MS.gene71597:CDS |
| TGAGTGGAGGGAATCGTTTG+GGG | 0.665615 | 1.4:+81975755 | None:intergenic |
| GTAGAAGATGAGGTCGCCTG+CGG | 0.679863 | 1.4:+81975722 | None:intergenic |
| GATGGTTGATGGGTCTGATG+AGG | 0.690508 | 1.4:-81975568 | MS.gene71597:CDS |
| ACTTTGGCTAAATCATCACC+GGG | 0.694568 | 1.4:+81974949 | None:intergenic |
| AAATTCTCCTAGATGACACG+GGG | 0.698131 | 1.4:+81974603 | None:intergenic |
| CTCATAATACCAGACCGACG+AGG | 0.724073 | 1.4:+81974751 | None:intergenic |
| GGAAGCCGCATAAAACACGG+TGG | 0.780935 | 1.4:+81975006 | None:intergenic |
| AATTCTCCTAGATGACACGG+GGG | 0.806379 | 1.4:+81974604 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTTGAAAATTCAGACTTT+AGG | - | chr1.4:81975257-81975276 | MS.gene71597:CDS | 20.0% |
| !! | ATTTGAAAATTCAGACTTTA+GGG | - | chr1.4:81975258-81975277 | MS.gene71597:CDS | 20.0% |
| !!! | TTTAATGGTTATTTTAACGA+TGG | - | chr1.4:81975398-81975417 | MS.gene71597:CDS | 20.0% |
| ! | CTGGAAAATTCTGAATTTAA+TGG | - | chr1.4:81975383-81975402 | MS.gene71597:CDS | 25.0% |
| ! | GGGTTTATTTAGATAAATCT+AGG | - | chr1.4:81975336-81975355 | MS.gene71597:CDS | 25.0% |
| ! | TCACAAAGATCAATTAATAG+TGG | + | chr1.4:81975476-81975495 | None:intergenic | 25.0% |
| !! | GAGATAAAGTTTTTGAATTG+TGG | - | chr1.4:81975423-81975442 | MS.gene71597:CDS | 25.0% |
| ATTGATCTTTGTGATAAAGC+TGG | - | chr1.4:81975482-81975501 | MS.gene71597:CDS | 30.0% | |
| GTATTATGAGGTTCTTTCAA+AGG | - | chr1.4:81975783-81975802 | MS.gene71597:CDS | 30.0% | |
| ! | AATCAGGAACTTCGATTTTA+AGG | + | chr1.4:81974638-81974657 | None:intergenic | 30.0% |
| ! | AGAAGATTTCCTAATCGTTT+TGG | - | chr1.4:81975806-81975825 | MS.gene71597:CDS | 30.0% |
| ! | ATCAGGAACTTCGATTTTAA+GGG | + | chr1.4:81974637-81974656 | None:intergenic | 30.0% |
| ! | GGGAATTTTGTGAATGTTTA+TGG | - | chr1.4:81975278-81975297 | MS.gene71597:CDS | 30.0% |
| ! | TTTTCCAGCATCAATTCTAA+AGG | + | chr1.4:81975371-81975390 | None:intergenic | 30.0% |
| !! | TGTGAATTTGAGTTGTTGAA+AGG | + | chr1.4:81974667-81974686 | None:intergenic | 30.0% |
| !!! | AATGGTTATTTTAACGATGG+TGG | - | chr1.4:81975401-81975420 | MS.gene71597:CDS | 30.0% |
| AACTTTGGCTAAATCATCAC+CGG | + | chr1.4:81975576-81975595 | None:intergenic | 35.0% | |
| AGAACATGTGCAACAAACTT+TGG | + | chr1.4:81975591-81975610 | None:intergenic | 35.0% | |
| ATAGAAGAGGAAGAAGATGA+AGG | + | chr1.4:81974705-81974724 | None:intergenic | 35.0% | |
| CATGCTGTTATTCTTGAATC+AGG | - | chr1.4:81975095-81975114 | MS.gene71597:CDS | 35.0% | |
| CTGATATTTGAGTATCTTCC+CGG | - | chr1.4:81975554-81975573 | MS.gene71597:CDS | 35.0% | |
| CTTCATCGATGATATCTTTG+AGG | - | chr1.4:81975189-81975208 | MS.gene71597:CDS | 35.0% | |
| CTTCCTGAGATTCTTACTAA+TGG | - | chr1.4:81975218-81975237 | MS.gene71597:CDS | 35.0% | |
| GAACCATTAGTAAGAATCTC+AGG | + | chr1.4:81975224-81975243 | None:intergenic | 35.0% | |
| GGATTATTCTTTACAGCCAT+TGG | + | chr1.4:81974993-81975012 | None:intergenic | 35.0% | |
| TAGAAGAGGAAGAAGATGAA+GGG | + | chr1.4:81974704-81974723 | None:intergenic | 35.0% | |
| TATGATCTGCAGCCAAATTT+CGG | - | chr1.4:81975854-81975873 | MS.gene71597:CDS | 35.0% | |
| TGAAATTCTCCTAGATGACA+CGG | + | chr1.4:81975923-81975942 | None:intergenic | 35.0% | |
| TGTGGAAGATAGTGAAAGAT+GGG | - | chr1.4:81975441-81975460 | MS.gene71597:CDS | 35.0% | |
| TGTTGAAAGGAACATGAATC+AGG | + | chr1.4:81974654-81974673 | None:intergenic | 35.0% | |
| TTGTGGAAGATAGTGAAAGA+TGG | - | chr1.4:81975440-81975459 | MS.gene71597:CDS | 35.0% | |
| TTTCGTGAAGAGGGTTATTA+AGG | - | chr1.4:81975022-81975041 | MS.gene71597:CDS | 35.0% | |
| ! | AGAACTTGTTTGCTCAGTAT+CGG | - | chr1.4:81975705-81975724 | MS.gene71597:CDS | 35.0% |
| ! | GATTTCGTCTTTTCTGTTGA+GGG | + | chr1.4:81974736-81974755 | None:intergenic | 35.0% |
| ! | TCGGAGATTTTTCGTCAATT+GGG | + | chr1.4:81974902-81974921 | None:intergenic | 35.0% |
| ! | TGATTTCGTCTTTTCTGTTG+AGG | + | chr1.4:81974737-81974756 | None:intergenic | 35.0% |
| ! | TGTTAGTGATTTGAAGCTGT+TGG | - | chr1.4:81975061-81975080 | MS.gene71597:CDS | 35.0% |
| ! | TTCTTTTCCAAAATCCTCGT+CGG | - | chr1.4:81975756-81975775 | MS.gene71597:CDS | 35.0% |
| !! | CGAGGATTTTGGAAAAGAAT+TGG | + | chr1.4:81975755-81975774 | None:intergenic | 35.0% |
| !! | TAAGCCTTTAGAATTGATGC+TGG | - | chr1.4:81975364-81975383 | MS.gene71597:CDS | 35.0% |
| !! | TTTGAATGATGGGTTTGTGA+GGG | + | chr1.4:81974863-81974882 | None:intergenic | 35.0% |
| !!! | TTTTGAATGATGGGTTTGTG+AGG | + | chr1.4:81974864-81974883 | None:intergenic | 35.0% |
| !!! | TTTTGGAATGCCTCCAATTT+GGG | - | chr1.4:81975823-81975842 | MS.gene71597:CDS | 35.0% |
| AAATTCTCCTAGATGACACG+GGG | + | chr1.4:81975921-81975940 | None:intergenic | 40.0% | |
| AAGAAGTTTGAGGAGGAGTT+TGG | - | chr1.4:81975650-81975669 | MS.gene71597:CDS | 40.0% | |
| AATGACACAGAGATGGTTGA+TGG | - | chr1.4:81974942-81974961 | MS.gene71597:CDS | 40.0% | |
| ACCCATCATTCAAAACTCAC+CGG | - | chr1.4:81974869-81974888 | MS.gene71597:CDS | 40.0% | |
| ACTTTGGCTAAATCATCACC+GGG | + | chr1.4:81975575-81975594 | None:intergenic | 40.0% | |
| AGAACCTGATTTCGTGAAGA+GGG | - | chr1.4:81975013-81975032 | MS.gene71597:CDS | 40.0% | |
| AGAAGTGAATGACACAGAGA+TGG | - | chr1.4:81974935-81974954 | MS.gene71597:CDS | 40.0% | |
| ATAACCCTCTTCACGAAATC+AGG | + | chr1.4:81975020-81975039 | None:intergenic | 40.0% | |
| ATGACACAGAGATGGTTGAT+GGG | - | chr1.4:81974943-81974962 | MS.gene71597:CDS | 40.0% | |
| ATTCTTTACAGCCATTGGCA+TGG | + | chr1.4:81974988-81975007 | None:intergenic | 40.0% | |
| ATTGGAAGCCGCATAAAACA+CGG | + | chr1.4:81975521-81975540 | None:intergenic | 40.0% | |
| CTTCCACAAGTCATCATTTG+AGG | + | chr1.4:81975630-81975649 | None:intergenic | 40.0% | |
| CTTGTGGAAGAAGAAGTTTG+AGG | - | chr1.4:81975640-81975659 | MS.gene71597:CDS | 40.0% | |
| GAAATTCTCCTAGATGACAC+GGG | + | chr1.4:81975922-81975941 | None:intergenic | 40.0% | |
| GGGAGATTTACACCGAAATT+TGG | + | chr1.4:81975869-81975888 | None:intergenic | 40.0% | |
| TTGCCTCAAATGATGACTTG+TGG | - | chr1.4:81975624-81975643 | MS.gene71597:CDS | 40.0% | |
| ! | AACTTCCACCGTGTTTTATG+CGG | - | chr1.4:81975510-81975529 | MS.gene71597:CDS | 40.0% |
| ! | ACAAGTCATCATTTGAGGCA+AGG | + | chr1.4:81975625-81975644 | None:intergenic | 40.0% |
| ! | ATCTCTGTGTCATTCACTTC+TGG | + | chr1.4:81974936-81974955 | None:intergenic | 40.0% |
| ! | CGGAGATTTTTCGTCAATTG+GGG | + | chr1.4:81974901-81974920 | None:intergenic | 40.0% |
| ! | GAACTTGTTTGCTCAGTATC+GGG | - | chr1.4:81975706-81975725 | MS.gene71597:CDS | 40.0% |
| ! | GGTTCATTATGTGATGATGC+TGG | - | chr1.4:81975299-81975318 | MS.gene71597:CDS | 40.0% |
| ! | GGTTGAAAGTGTAGAAGATG+AGG | + | chr1.4:81974812-81974831 | None:intergenic | 40.0% |
| ! | GTCGGAGATTTTTCGTCAAT+TGG | + | chr1.4:81974903-81974922 | None:intergenic | 40.0% |
| ! | TCTCTGTGTCATTCACTTCT+GGG | + | chr1.4:81974935-81974954 | None:intergenic | 40.0% |
| ! | TTTGAAGCTGTTGGTGTTTG+CGG | - | chr1.4:81975070-81975089 | MS.gene71597:CDS | 40.0% |
| ! | TTTGGAATGCCTCCAATTTG+GGG | - | chr1.4:81975824-81975843 | MS.gene71597:CDS | 40.0% |
| !!! | GTTTTGGAATGCCTCCAATT+TGG | - | chr1.4:81975822-81975841 | MS.gene71597:CDS | 40.0% |
| !!! | TCCGGTGAGTTTTGAATGAT+GGG | + | chr1.4:81974873-81974892 | None:intergenic | 40.0% |
| !!! | TGAATCAGGTTTTGTTCGTG+TGG | - | chr1.4:81975109-81975128 | MS.gene71597:CDS | 40.0% |
| !!! | TTCCGGTGAGTTTTGAATGA+TGG | + | chr1.4:81974874-81974893 | None:intergenic | 40.0% |
| AACGATTCCCTCCACTCAAT+CGG | - | chr1.4:81974771-81974790 | MS.gene71597:CDS | 45.0% | |
| AATTCTCCTAGATGACACGG+GGG | + | chr1.4:81975920-81975939 | None:intergenic | 45.0% | |
| AGAGGAAGAAGATGAAGGGA+TGG | + | chr1.4:81974700-81974719 | None:intergenic | 45.0% | |
| ATCAGCAGCTTAAGCTCCAT+TGG | + | chr1.4:81975539-81975558 | None:intergenic | 45.0% | |
| ATTGAGTGGAGGGAATCGTT+TGG | + | chr1.4:81974771-81974790 | None:intergenic | 45.0% | |
| CAGAACCTGATTTCGTGAAG+AGG | - | chr1.4:81975012-81975031 | MS.gene71597:CDS | 45.0% | |
| CTCTTCACGAAATCAGGTTC+TGG | + | chr1.4:81975014-81975033 | None:intergenic | 45.0% | |
| GAGGGAGAGATGAATAGAAG+AGG | + | chr1.4:81974718-81974737 | None:intergenic | 45.0% | |
| GTGGAAGAAGAAGTTTGAGG+AGG | - | chr1.4:81975643-81975662 | MS.gene71597:CDS | 45.0% | |
| TCATACTCTCCACCCCAAAT+TGG | + | chr1.4:81975839-81975858 | None:intergenic | 45.0% | |
| TCGTCAATTGGGGTGATTTC+CGG | + | chr1.4:81974891-81974910 | None:intergenic | 45.0% | |
| TGTTCGTGTGGACAAGGATT+CGG | - | chr1.4:81975121-81975140 | MS.gene71597:CDS | 45.0% | |
| TTGAGGAGGAGTTTGGACAA+AGG | - | chr1.4:81975657-81975676 | MS.gene71597:CDS | 45.0% | |
| TTGAGTGGAGGGAATCGTTT+GGG | + | chr1.4:81974770-81974789 | None:intergenic | 45.0% | |
| ! | CTCTGTGTCATTCACTTCTG+GGG | + | chr1.4:81974934-81974953 | None:intergenic | 45.0% |
| ! | GTGTTTTATGCGGCTTCCAA+TGG | - | chr1.4:81975520-81975539 | MS.gene71597:CDS | 45.0% |
| !! | AATGATGGGTTTGTGAGGGA+GGG | + | chr1.4:81974859-81974878 | None:intergenic | 45.0% |
| !! | ACTTCTGGGGTGTTCAAAGT+CGG | + | chr1.4:81974921-81974940 | None:intergenic | 45.0% |
| !! | AGGGTTATTAAGGAAGCGCT+CGG | - | chr1.4:81975032-81975051 | MS.gene71597:CDS | 45.0% |
| !! | ATGTGATGATGCTGGTTCGA+GGG | - | chr1.4:81975307-81975326 | MS.gene71597:CDS | 45.0% |
| !! | GGAGGCATTCCAAAACGATT+AGG | + | chr1.4:81975818-81975837 | None:intergenic | 45.0% |
| !! | TATGTGATGATGCTGGTTCG+AGG | - | chr1.4:81975306-81975325 | MS.gene71597:CDS | 45.0% |
| !!! | AGGTTTTGTTCGTGTGGACA+AGG | - | chr1.4:81975115-81975134 | MS.gene71597:CDS | 45.0% |
| AGGAGGAGTTTGGACAAAGG+AGG | - | chr1.4:81975660-81975679 | MS.gene71597:CDS | 50.0% | |
| CTCATAATACCAGACCGACG+AGG | + | chr1.4:81975773-81975792 | None:intergenic | 50.0% | |
| CTCGTCGGTCTGGTATTATG+AGG | - | chr1.4:81975771-81975790 | MS.gene71597:CDS | 50.0% | |
| GGAGGAGTTTGGACAAAGGA+GGG | - | chr1.4:81975661-81975680 | MS.gene71597:CDS | 50.0% | |
| GTTCGTGTGGACAAGGATTC+GGG | - | chr1.4:81975122-81975141 | MS.gene71597:CDS | 50.0% | |
| TACTCTCCACCCCAAATTGG+AGG | + | chr1.4:81975836-81975855 | None:intergenic | 50.0% | |
| TGAGTGGAGGGAATCGTTTG+GGG | + | chr1.4:81974769-81974788 | None:intergenic | 50.0% | |
| TGTGGACAAGGATTCGGGTA+TGG | - | chr1.4:81975127-81975146 | MS.gene71597:CDS | 50.0% | |
| TTCATTCCCCCGTGTCATCT+AGG | - | chr1.4:81975911-81975930 | MS.gene71597:CDS | 50.0% | |
| TTCCAAAATCCTCGTCGGTC+TGG | - | chr1.4:81975761-81975780 | MS.gene71597:CDS | 50.0% | |
| ! | TACCAGACCGACGAGGATTT+TGG | + | chr1.4:81975766-81975785 | None:intergenic | 50.0% |
| !! | CGTTGTGGGAGAAAGCGTTA+GGG | + | chr1.4:81974833-81974852 | None:intergenic | 50.0% |
| !! | GAATGATGGGTTTGTGAGGG+AGG | + | chr1.4:81974860-81974879 | None:intergenic | 50.0% |
| !! | GATGGTTGATGGGTCTGATG+AGG | - | chr1.4:81974953-81974972 | MS.gene71597:CDS | 50.0% |
| !! | TCGTTGTGGGAGAAAGCGTT+AGG | + | chr1.4:81974834-81974853 | None:intergenic | 50.0% |
| ATCTCCCTGCATACGCACCA+AGG | - | chr1.4:81975882-81975901 | MS.gene71597:CDS | 55.0% | |
| GGAAGCCGCATAAAACACGG+TGG | + | chr1.4:81975518-81975537 | None:intergenic | 55.0% | |
| GGAATGCCTCCAATTTGGGG+TGG | - | chr1.4:81975827-81975846 | MS.gene71597:CDS | 55.0% | |
| GTAGAAGATGAGGTCGCCTG+CGG | + | chr1.4:81974802-81974821 | None:intergenic | 55.0% | |
| GTGAGGGAGGGTTTCGTTGT+GGG | + | chr1.4:81974847-81974866 | None:intergenic | 55.0% | |
| TGTGAGGGAGGGTTTCGTTG+TGG | + | chr1.4:81974848-81974867 | None:intergenic | 55.0% | |
| ! | GGGGGAATGAAAGTACGCCT+TGG | + | chr1.4:81975902-81975921 | None:intergenic | 55.0% |
| ! | TACGCCTTGGTGCGTATGCA+GGG | + | chr1.4:81975889-81975908 | None:intergenic | 55.0% |
| !! | ATGCTGGTTCGAGGGTGCAT+AGG | - | chr1.4:81975315-81975334 | MS.gene71597:CDS | 55.0% |
| !! | GGTTGATGGGTCTGATGAGG+CGG | - | chr1.4:81974956-81974975 | MS.gene71597:CDS | 55.0% |
| !! | TGCTGGTTCGAGGGTGCATA+GGG | - | chr1.4:81975316-81975335 | MS.gene71597:CDS | 55.0% |
| GTGGAGGGAATCGTTTGGGG+AGG | + | chr1.4:81974766-81974785 | None:intergenic | 60.0% | |
| ! | GGCGATGCCGATTGAGTGGA+GGG | + | chr1.4:81974781-81974800 | None:intergenic | 60.0% |
| ! | GTACGCCTTGGTGCGTATGC+AGG | + | chr1.4:81975890-81975909 | None:intergenic | 60.0% |
| ! | TGATGGGTCTGATGAGGCGG+CGG | - | chr1.4:81974959-81974978 | MS.gene71597:CDS | 60.0% |
| CACTCAATCGGCATCGCCGC+AGG | - | chr1.4:81974783-81974802 | MS.gene71597:CDS | 65.0% | |
| ! | CGGCGATGCCGATTGAGTGG+AGG | + | chr1.4:81974782-81974801 | None:intergenic | 65.0% |
| ! | CTGCGGCGATGCCGATTGAG+TGG | + | chr1.4:81974785-81974804 | None:intergenic | 65.0% |
| GGCGGCGGTGACCATGCCAA+TGG | - | chr1.4:81974974-81974993 | MS.gene71597:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 81974597 | 81975946 | 81974597 | ID=MS.gene71597 |
| chr1.4 | mRNA | 81974597 | 81975946 | 81974597 | ID=MS.gene71597.t1;Parent=MS.gene71597 |
| chr1.4 | exon | 81974597 | 81975946 | 81974597 | ID=MS.gene71597.t1.exon1;Parent=MS.gene71597.t1 |
| chr1.4 | CDS | 81974597 | 81975946 | 81974597 | ID=cds.MS.gene71597.t1;Parent=MS.gene71597.t1 |
| Gene Sequence |
| Protein sequence |
