AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene99858


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99858.t1 MTR_2g105640 99.020 102 1 0 1 102 269 370 7.61e-63 197
MS.gene99858.t1 MTR_3g082180 61.053 95 37 0 8 102 268 362 4.47e-33 119
MS.gene99858.t1 MTR_4g016720 55.446 101 38 1 9 102 267 367 1.69e-30 112
MS.gene99858.t1 MTR_5g070330 58.416 101 41 1 3 102 258 358 6.75e-29 107
MS.gene99858.t1 MTR_7g068380 65.823 79 27 0 24 102 289 367 8.01e-29 107
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99858.t1 AT3G13320 73.196 97 26 0 6 102 273 369 1.64e-41 141
MS.gene99858.t1 AT1G55730 72.165 97 27 0 6 102 273 369 1.78e-41 141
MS.gene99858.t1 AT1G55730 72.165 97 27 0 6 102 273 369 1.78e-41 141
MS.gene99858.t1 AT1G55720 69.072 97 30 0 6 102 281 377 9.70e-40 137
MS.gene99858.t1 AT1G55720 69.072 97 30 0 6 102 281 377 1.11e-39 137
MS.gene99858.t1 AT5G01490 54.369 103 46 1 1 102 271 373 3.13e-29 108
MS.gene99858.t1 AT5G01490 54.369 103 46 1 1 102 271 373 3.13e-29 108
MS.gene99858.t1 AT3G51860 65.823 79 27 0 24 102 293 371 5.71e-28 105
MS.gene99858.t1 AT3G51860 65.823 79 27 0 24 102 293 371 6.46e-28 105
MS.gene99858.t1 AT2G38170 52.336 107 43 2 3 102 264 369 2.83e-27 103
MS.gene99858.t1 AT2G38170 52.336 107 43 2 3 102 264 369 2.90e-27 103
MS.gene99858.t1 AT2G38170 48.864 88 37 2 3 83 264 350 9.92e-18 77.0

Find 20 sgRNAs with CRISPR-Local

Find 113 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCAATCGGAAGCGATAATT+TGG 0.177448 2.1:+301728 MS.gene99858:CDS
ATACCACAAACATCGATATC+TGG 0.329138 2.1:-303199 None:intergenic
TGCTATCATGTTTGCTGTAA+AGG 0.362095 2.1:+303058 MS.gene99858:CDS
GTCATCTTGCTTCCAATTGT+AGG 0.377243 2.1:+303014 MS.gene99858:CDS
TTTCCACAGGATATCACAAT+TGG 0.399751 2.1:+303155 MS.gene99858:intron
TCTGCGGCATTTCCTACAAT+TGG 0.429263 2.1:-303026 None:intergenic
ATCACAATTGGAGTTGCTGT+TGG 0.467118 2.1:+303167 MS.gene99858:CDS
ACTGTACAGCTATACCTCTA+TGG 0.490233 2.1:-301803 None:intergenic
GTATGGGTATCAGTGTTGTC+TGG 0.492249 2.1:+301766 MS.gene99858:CDS
AGAACTTGAGTTAACTCAAT+CGG 0.503816 2.1:+301714 MS.gene99858:CDS
AACCCAGATATCGATGTTTG+TGG 0.505182 2.1:+303196 MS.gene99858:CDS
AGATATCGATGTTTGTGGTA+TGG 0.534307 2.1:+303201 MS.gene99858:CDS
TACCACAAACATCGATATCT+GGG 0.548709 2.1:-303198 None:intergenic
ATACCTTGTGGATGCCATAG+AGG 0.575475 2.1:+301789 MS.gene99858:CDS
TGAATCATTGAATATGTCAG+TGG 0.638115 2.1:+302980 MS.gene99858:CDS
AAGTGAAAACTCTGACGAGG+AGG 0.660035 2.1:+301690 MS.gene99858:CDS
AGTGTTGTCTGGATACCTTG+TGG 0.676735 2.1:+301777 MS.gene99858:CDS
ATACCTCTATGGCATCCACA+AGG 0.684822 2.1:-301792 None:intergenic
TACAAGTGAAAACTCTGACG+AGG 0.708110 2.1:+301687 MS.gene99858:CDS
GATAGCACTTGCATGCTCTG+CGG 0.744200 2.1:-303042 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTATTTACCCAAAATTAT+GGG - chr2.1:302045-302064 None:intergenic 15.0%
!! GTATTCATGGAATAAAATAT+AGG - chr2.1:303109-303128 None:intergenic 20.0%
!!! CTTTTATTTACCCAAAATTA+TGG - chr2.1:302046-302065 None:intergenic 20.0%
!!! TCATTTATACCCATAATTTT+GGG + chr2.1:302033-302052 MS.gene99858:intron 20.0%
! AAAGTCATGAAAAGGAAATA+TGG + chr2.1:302722-302741 MS.gene99858:intron 25.0%
! AATACTGTTTAATCCGTTAA+TGG + chr2.1:303124-303143 MS.gene99858:intron 25.0%
! ATACTTTCTGATAGATCATT+AGG - chr2.1:302437-302456 None:intergenic 25.0%
! TCGTAAATTTAATCTGTTGT+TGG + chr2.1:302507-302526 MS.gene99858:intron 25.0%
! TCTGAATAGTACCAAAAATA+TGG - chr2.1:302154-302173 None:intergenic 25.0%
!! AATACTCTGTTTTATAGCAT+GGG - chr2.1:302677-302696 None:intergenic 25.0%
!! ATCATTAGGATTTTCTATGT+TGG - chr2.1:302423-302442 None:intergenic 25.0%
!! CTCATTTATACCCATAATTT+TGG + chr2.1:302032-302051 MS.gene99858:intron 25.0%
!! GCTATAAAACAGAGTATTAT+TGG + chr2.1:302679-302698 MS.gene99858:intron 25.0%
!! GTAAATAAAAGAAGCATGTA+TGG + chr2.1:302055-302074 MS.gene99858:intron 25.0%
!! TAATACTCTGTTTTATAGCA+TGG - chr2.1:302678-302697 None:intergenic 25.0%
!!! GCATTGAGTTTTTAAGAATT+GGG + chr2.1:302221-302240 MS.gene99858:intron 25.0%
AAAAGGAAATATGGAAACAG+AGG + chr2.1:302731-302750 MS.gene99858:intron 30.0%
AGAACTTGAGTTAACTCAAT+CGG + chr2.1:301714-301733 MS.gene99858:CDS 30.0%
AGAATGAATAGAAGTTTCAG+TGG - chr2.1:302885-302904 None:intergenic 30.0%
ATACCAGGACCAAAAATAAA+AGG - chr2.1:302600-302619 None:intergenic 30.0%
CCAAAAATAAAAGGCTGATA+AGG - chr2.1:302591-302610 None:intergenic 30.0%
GAGTATTATTGGAAATCATG+AGG + chr2.1:302690-302709 MS.gene99858:intron 30.0%
GGAAAAGTTGAATCCATTAA+CGG - chr2.1:303140-303159 None:intergenic 30.0%
TACTATTCAGAAGAAACGAT+GGG + chr2.1:302163-302182 MS.gene99858:intron 30.0%
TGAATCATTGAATATGTCAG+TGG + chr2.1:302980-302999 MS.gene99858:CDS 30.0%
TTAAGAATTGGGTGAAATAG+TGG + chr2.1:302232-302251 MS.gene99858:intron 30.0%
TTATTGAAATCTTAGTGAGC+TGG - chr2.1:302381-302400 None:intergenic 30.0%
!! AACGGATTAAACAGTATTCA+TGG - chr2.1:303122-303141 None:intergenic 30.0%
!! AGGATTCTAAAGTCATGAAA+AGG + chr2.1:302714-302733 MS.gene99858:intron 30.0%
!! AGTGATGATGACTTTTGATA+TGG + chr2.1:302199-302218 MS.gene99858:intron 30.0%
!! GATCTATCAGAAAGTATTTG+TGG + chr2.1:302440-302459 MS.gene99858:intron 30.0%
!! GGCATTGAGTTTTTAAGAAT+TGG + chr2.1:302220-302239 MS.gene99858:intron 30.0%
!!! AATTTGGCTTGCAATTTTGA+CGG + chr2.1:301744-301763 MS.gene99858:CDS 30.0%
!!! CCTTATCAGCCTTTTATTTT+TGG + chr2.1:302588-302607 MS.gene99858:intron 30.0%
!!! TTAAGCTTTTGTTATTCTCG+TGG + chr2.1:301848-301867 MS.gene99858:intron 30.0%
AACATGAAGCGAACACTAAA+AGG - chr2.1:301889-301908 None:intergenic 35.0%
AACCATTTACACCAGTTGAA+GGG + chr2.1:302124-302143 MS.gene99858:intron 35.0%
AAGATGCTTATGGATCAACA+AGG - chr2.1:302932-302951 None:intergenic 35.0%
ACACGATTCTATGGTGTAAT+TGG - chr2.1:301950-301969 None:intergenic 35.0%
AGATATCGATGTTTGTGGTA+TGG + chr2.1:303201-303220 MS.gene99858:CDS 35.0%
ATACCACAAACATCGATATC+TGG - chr2.1:303202-303221 None:intergenic 35.0%
ATTACACCATAGAATCGTGT+CGG + chr2.1:301950-301969 MS.gene99858:intron 35.0%
ATTATTGGAAATCATGAGGC+AGG + chr2.1:302694-302713 MS.gene99858:intron 35.0%
GTACTATTCAGAAGAAACGA+TGG + chr2.1:302162-302181 MS.gene99858:intron 35.0%
GTATCATGTAACAGTGTGTT+AGG - chr2.1:302095-302114 None:intergenic 35.0%
TAACCATTTACACCAGTTGA+AGG + chr2.1:302123-302142 MS.gene99858:intron 35.0%
TACCACAAACATCGATATCT+GGG - chr2.1:303201-303220 None:intergenic 35.0%
TCTAGATCTAGATCAGATCA+AGG + chr2.1:302340-302359 MS.gene99858:intron 35.0%
TTTCCACAGGATATCACAAT+TGG + chr2.1:303155-303174 MS.gene99858:intron 35.0%
! TGAAGGGAGTTCCATATTTT+TGG + chr2.1:302140-302159 MS.gene99858:intron 35.0%
! TTGATCTTTGACTTTGACCA+AGG - chr2.1:301995-302014 None:intergenic 35.0%
!! AATGGATTCAACTTTTCCAC+AGG + chr2.1:303142-303161 MS.gene99858:intron 35.0%
!! TGCTATCATGTTTGCTGTAA+AGG + chr2.1:303058-303077 MS.gene99858:CDS 35.0%
!!! ATTTTTGGTCCTGGTATGAA+TGG + chr2.1:302603-302622 MS.gene99858:intron 35.0%
AAAAGGCTGATAAGGAGAAG+AGG - chr2.1:302583-302602 None:intergenic 40.0%
AACCCAGATATCGATGTTTG+TGG + chr2.1:303196-303215 MS.gene99858:CDS 40.0%
AATATGGAAACAGAGGAGGA+AGG + chr2.1:302738-302757 MS.gene99858:intron 40.0%
AATATGGAACTCCCTTCAAC+TGG - chr2.1:302138-302157 None:intergenic 40.0%
ACGAGTCGAAATACAATGTG+TGG - chr2.1:302491-302510 None:intergenic 40.0%
ACTCCAATTGTGATATCCTG+TGG - chr2.1:303161-303180 None:intergenic 40.0%
ACTGTACAGCTATACCTCTA+TGG - chr2.1:301806-301825 None:intergenic 40.0%
AGGAAATATGGAAACAGAGG+AGG + chr2.1:302734-302753 MS.gene99858:intron 40.0%
ATATGGAAACAGAGGAGGAA+GGG + chr2.1:302739-302758 MS.gene99858:intron 40.0%
ATCACAATTGGAGTTGCTGT+TGG + chr2.1:303167-303186 MS.gene99858:CDS 40.0%
CAATGTCTATGGGTTTACCT+TGG + chr2.1:301975-301994 MS.gene99858:intron 40.0%
CCAGTAACACAAGATGCTTA+TGG - chr2.1:302942-302961 None:intergenic 40.0%
CCATAAGCATCTTGTGTTAC+TGG + chr2.1:302939-302958 MS.gene99858:intron 40.0%
CTCAATCGGAAGCGATAATT+TGG + chr2.1:301728-301747 MS.gene99858:CDS 40.0%
GGAGTTCTTCATTGTCAACA+AGG - chr2.1:302911-302930 None:intergenic 40.0%
GTAAAGGACAAGCTAGTAAG+TGG + chr2.1:303074-303093 MS.gene99858:intron 40.0%
GTCATCTTGCTTCCAATTGT+AGG + chr2.1:303014-303033 MS.gene99858:CDS 40.0%
TACAAGTGAAAACTCTGACG+AGG + chr2.1:301687-301706 MS.gene99858:CDS 40.0%
TTACCCACCCAAAAACTACA+CGG + chr2.1:302799-302818 MS.gene99858:intron 40.0%
TTCAGAAGAAACGATGGGAT+TGG + chr2.1:302168-302187 MS.gene99858:intron 40.0%
TTTGGGTGGGTAAGGTTTAT+GGG - chr2.1:302792-302811 None:intergenic 40.0%
! TCTGTTGCCGTGTAGTTTTT+GGG - chr2.1:302809-302828 None:intergenic 40.0%
! TGTGTTACTGGTCTATTGCA+GGG + chr2.1:302951-302970 MS.gene99858:intron 40.0%
! TTCTGTTGCCGTGTAGTTTT+TGG - chr2.1:302810-302829 None:intergenic 40.0%
! TTGTGTTACTGGTCTATTGC+AGG + chr2.1:302950-302969 MS.gene99858:intron 40.0%
! TTTTATAGCATGGGCCAATG+TGG - chr2.1:302668-302687 None:intergenic 40.0%
!! GCTTGCAATTTTGACGGTAT+GGG + chr2.1:301750-301769 MS.gene99858:CDS 40.0%
!! GTTGTTGGTTAAGCTGCTTT+TGG + chr2.1:302522-302541 MS.gene99858:intron 40.0%
!! TTTTGGGTGGGTAAGGTTTA+TGG - chr2.1:302793-302812 None:intergenic 40.0%
!!! CAGCCTTTTATTTTTGGTCC+TGG + chr2.1:302594-302613 MS.gene99858:intron 40.0%
AAGTGAAAACTCTGACGAGG+AGG + chr2.1:301690-301709 MS.gene99858:CDS 45.0%
AGTGTTGTCTGGATACCTTG+TGG + chr2.1:301777-301796 MS.gene99858:CDS 45.0%
ATACCTCTATGGCATCCACA+AGG - chr2.1:301795-301814 None:intergenic 45.0%
ATACCTTGTGGATGCCATAG+AGG + chr2.1:301789-301808 MS.gene99858:CDS 45.0%
CTCCCTTCAACTGGTGTAAA+TGG - chr2.1:302129-302148 None:intergenic 45.0%
GTATGGGTATCAGTGTTGTC+TGG + chr2.1:301766-301785 MS.gene99858:CDS 45.0%
TCTGCGGCATTTCCTACAAT+TGG - chr2.1:303029-303048 None:intergenic 45.0%
TGTGTTAGGTGATGTTGAGC+TGG - chr2.1:302081-302100 None:intergenic 45.0%
! ATTGCACCGACACGATTCTA+TGG - chr2.1:301959-301978 None:intergenic 45.0%
! TTGGGTGAAATAGTGGAGAG+TGG + chr2.1:302239-302258 MS.gene99858:intron 45.0%
!! GGCTTGCAATTTTGACGGTA+TGG + chr2.1:301749-301768 MS.gene99858:CDS 45.0%
!!! GTGTAGTTTTTGGGTGGGTA+AGG - chr2.1:302800-302819 None:intergenic 45.0%
!!! TTGCCGTGTAGTTTTTGGGT+GGG - chr2.1:302805-302824 None:intergenic 45.0%
AACTCCTGCCACATCCACAT+TGG + chr2.1:302651-302670 MS.gene99858:intron 50.0%
GATAGCACTTGCATGCTCTG+CGG - chr2.1:303045-303064 None:intergenic 50.0%
GTCCAGAGTCCATTCATACC+AGG - chr2.1:302615-302634 None:intergenic 50.0%
GTGGGTAAGGTTTATGGGCA+AGG - chr2.1:302787-302806 None:intergenic 50.0%
! CGTGTCGGTGCAATGTCTAT+GGG + chr2.1:301965-301984 MS.gene99858:intron 50.0%
! GATCAAGGACTAGCAGCTCT+AGG + chr2.1:302355-302374 MS.gene99858:intron 50.0%
! GTCCTGGTATGAATGGACTC+TGG + chr2.1:302610-302629 MS.gene99858:intron 50.0%
! TCGTGTCGGTGCAATGTCTA+TGG + chr2.1:301964-301983 MS.gene99858:intron 50.0%
!! GTTGCCGTGTAGTTTTTGGG+TGG - chr2.1:302806-302825 None:intergenic 50.0%
AGCATGGGCCAATGTGGATG+TGG - chr2.1:302662-302681 None:intergenic 55.0%
GGACTCTGGACCATTCCCAA+GGG + chr2.1:302624-302643 MS.gene99858:intron 55.0%
GGAGTTGCTTCCCTTGGGAA+TGG - chr2.1:302637-302656 None:intergenic 55.0%
TGGACTCTGGACCATTCCCA+AGG + chr2.1:302623-302642 MS.gene99858:intron 55.0%
TGGCAGGAGTTGCTTCCCTT+GGG - chr2.1:302642-302661 None:intergenic 55.0%
GTGGCAGGAGTTGCTTCCCT+TGG - chr2.1:302643-302662 None:intergenic 60.0%
TGGGCCAATGTGGATGTGGC+AGG - chr2.1:302658-302677 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 301679 303229 301679 ID=MS.gene99858
chr2.1 mRNA 301679 303229 301679 ID=MS.gene99858.t1;Parent=MS.gene99858
chr2.1 exon 301679 301810 301679 ID=MS.gene99858.t1.exon1;Parent=MS.gene99858.t1
chr2.1 CDS 301679 301810 301679 ID=cds.MS.gene99858.t1;Parent=MS.gene99858.t1
chr2.1 exon 302972 303088 302972 ID=MS.gene99858.t1.exon2;Parent=MS.gene99858.t1
chr2.1 CDS 302972 303088 302972 ID=cds.MS.gene99858.t1;Parent=MS.gene99858.t1
chr2.1 exon 303164 303229 303164 ID=MS.gene99858.t1.exon3;Parent=MS.gene99858.t1
chr2.1 CDS 303164 303229 303164 ID=cds.MS.gene99858.t1;Parent=MS.gene99858.t1
Gene Sequence

>MS.gene99858

GAAGTTGATACAAGTGAAAACTCTGACGAGGAGGAAGAACTTGAGTTAACTCAATCGGAAGCGATAATTTGGCTTGCAATTTTGACGGTATGGGTATCAGTGTTGTCTGGATACCTTGTGGATGCCATAGAGGTATAGCTGTACAGTCACTTTCAGAATTTCTCAATCATTAAGCTTTTGTTATTCTCGTGGAATGTTGAATTTTTTCCTTTTAGTGTTCGCTTCATGTTATTTTATTTTAGCATAAAACTTCAATCATAGAACATGTCCAATTACACCATAGAATCGTGTCGGTGCAATGTCTATGGGTTTACCTTGGTCAAAGTCAAAGATCAAATAAAGAAAAGTAAAAACTCATTTATACCCATAATTTTGGGTAAATAAAAGAAGCATGTATGGCCAGCTCAACATCACCTAACACACTGTTACATGATACAATAAAACTAACCATTTACACCAGTTGAAGGGAGTTCCATATTTTTGGTACTATTCAGAAGAAACGATGGGATTGGTTCATAAGAGTGATGATGACTTTTGATATGGCATTGAGTTTTTAAGAATTGGGTGAAATAGTGGAGAGTGGTATTTATTCACTAATCAGTTTCATTTATCAAATGATGCTGATATAAATGTCGACATTTTACTCATAATTGTACTAAAATCTAGATCTAGATCAGATCAAGGACTAGCAGCTCTAGGCCAGCTCACTAAGATTTCAATAAATTAATTCTATTATACTATCCAACATAGAAAATCCTAATGATCTATCAGAAAGTATTTGTGGTCATTTGTCTACTGTTTTTTATGAACCACACATTGTATTTCGACTCGTAAATTTAATCTGTTGTTGGTTAAGCTGCTTTTGGTTAATATTGCAATTATACTATTCATATATAGATAACCTCTTCTCCTTATCAGCCTTTTATTTTTGGTCCTGGTATGAATGGACTCTGGACCATTCCCAAGGGAAGCAACTCCTGCCACATCCACATTGGCCCATGCTATAAAACAGAGTATTATTGGAAATCATGAGGCAGGATTCTAAAGTCATGAAAAGGAAATATGGAAACAGAGGAGGAAGGGTCTAAACACAGTTTAATTTAATCCTTGCCCATAAACCTTACCCACCCAAAAACTACACGGCAACAGAAATTTAATGATTTCTTTGCGAGCTATGTTTGCTTTTTGATTCGAGATTATATACCACTGAAACTTCTATTCATTCTTGTCCTTGTTGACAATGAAGAACTCCTTGTTGATCCATAAGCATCTTGTGTTACTGGTCTATTGCAGGGAGCATCTGAATCATTGAATATGTCAGTGGCTTTTATTAGCGTCATCTTGCTTCCAATTGTAGGAAATGCCGCAGAGCATGCAAGTGCTATCATGTTTGCTGTAAAGGACAAGCTAGTAAGTGGCTTTCATAGCCTATATTTTATTCCATGAATACTGTTTAATCCGTTAATGGATTCAACTTTTCCACAGGATATCACAATTGGAGTTGCTGTTGGATCATCAACCCAGATATCGATGTTTGTGGTATGGCCTTGA

Protein sequence

>MS.gene99858.t1

EVDTSENSDEEEELELTQSEAIIWLAILTVWVSVLSGYLVDAIEGASESLNMSVAFISVILLPIVGNAAEHASAIMFAVKDKLDITIGVAVGSSTQISMFVVWP