Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60680.t1 | XP_003592116.1 | 97.2 | 386 | 11 | 0 | 1 | 386 | 1 | 386 | 6.30E-208 | 733.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60680.t1 | G7ICZ3 | 97.2 | 386 | 11 | 0 | 1 | 386 | 1 | 386 | 4.5e-208 | 733.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene60680 | 0.860507 | 1.97E-63 | -1.69E-46 |
| MS.gene049254 | MS.gene60680 | 0.816018 | 6.87E-52 | -1.69E-46 |
| MS.gene049404 | MS.gene60680 | 0.832766 | 7.84E-56 | -1.69E-46 |
| MS.gene049424 | MS.gene60680 | 0.803184 | 3.94E-49 | -1.69E-46 |
| MS.gene049435 | MS.gene60680 | 0.835194 | 1.93E-56 | -1.69E-46 |
| MS.gene049452 | MS.gene60680 | 0.845246 | 4.58E-59 | -1.69E-46 |
| MS.gene049501 | MS.gene60680 | 0.806337 | 8.64E-50 | -1.69E-46 |
| MS.gene049983 | MS.gene60680 | 0.843273 | 1.55E-58 | -1.69E-46 |
| MS.gene050073 | MS.gene60680 | 0.801768 | 7.70E-49 | -1.69E-46 |
| MS.gene050442 | MS.gene60680 | 0.82495 | 6.11E-54 | -1.69E-46 |
| MS.gene050559 | MS.gene60680 | 0.811009 | 8.67E-51 | -1.69E-46 |
| MS.gene050561 | MS.gene60680 | 0.810795 | 9.65E-51 | -1.69E-46 |
| MS.gene050605 | MS.gene60680 | 0.81691 | 4.33E-52 | -1.69E-46 |
| MS.gene050654 | MS.gene60680 | 0.80841 | 3.14E-50 | -1.69E-46 |
| MS.gene050680 | MS.gene60680 | 0.81135 | 7.32E-51 | -1.69E-46 |
| MS.gene050750 | MS.gene60680 | 0.81821 | 2.21E-52 | -1.69E-46 |
| MS.gene050759 | MS.gene60680 | 0.865485 | 5.71E-65 | -1.69E-46 |
| MS.gene050826 | MS.gene60680 | 0.818269 | 2.14E-52 | -1.69E-46 |
| MS.gene050953 | MS.gene60680 | 0.808831 | 2.55E-50 | -1.69E-46 |
| MS.gene050962 | MS.gene60680 | 0.855158 | 7.61E-62 | -1.69E-46 |
| MS.gene051198 | MS.gene60680 | 0.856863 | 2.41E-62 | -1.69E-46 |
| MS.gene051279 | MS.gene60680 | 0.820458 | 6.79E-53 | -1.69E-46 |
| MS.gene051328 | MS.gene60680 | 0.805733 | 1.16E-49 | -1.69E-46 |
| MS.gene051368 | MS.gene60680 | 0.827015 | 1.98E-54 | -1.69E-46 |
| MS.gene051744 | MS.gene60680 | 0.805009 | 1.64E-49 | -1.69E-46 |
| MS.gene051800 | MS.gene60680 | 0.825999 | 3.45E-54 | -1.69E-46 |
| MS.gene051924 | MS.gene60680 | -0.80033 | 1.52E-48 | -1.69E-46 |
| MS.gene052108 | MS.gene60680 | 0.801594 | 8.37E-49 | -1.69E-46 |
| MS.gene052516 | MS.gene60680 | 0.82359 | 1.28E-53 | -1.69E-46 |
| MS.gene052519 | MS.gene60680 | 0.816718 | 4.79E-52 | -1.69E-46 |
| MS.gene052582 | MS.gene60680 | 0.824202 | 9.17E-54 | -1.69E-46 |
| MS.gene052584 | MS.gene60680 | 0.814826 | 1.26E-51 | -1.69E-46 |
| MS.gene052674 | MS.gene60680 | 0.832394 | 9.70E-56 | -1.69E-46 |
| MS.gene052675 | MS.gene60680 | 0.832738 | 7.96E-56 | -1.69E-46 |
| MS.gene052677 | MS.gene60680 | 0.83581 | 1.35E-56 | -1.69E-46 |
| MS.gene052863 | MS.gene60680 | 0.809528 | 1.81E-50 | -1.69E-46 |
| MS.gene052954 | MS.gene60680 | 0.81146 | 6.92E-51 | -1.69E-46 |
| MS.gene053008 | MS.gene60680 | 0.832057 | 1.17E-55 | -1.69E-46 |
| MS.gene053079 | MS.gene60680 | 0.828367 | 9.35E-55 | -1.69E-46 |
| MS.gene053166 | MS.gene60680 | 0.820828 | 5.58E-53 | -1.69E-46 |
| MS.gene053226 | MS.gene60680 | 0.818857 | 1.57E-52 | -1.69E-46 |
| MS.gene053931 | MS.gene60680 | 0.828508 | 8.65E-55 | -1.69E-46 |
| MS.gene05415 | MS.gene60680 | 0.836107 | 1.14E-56 | -1.69E-46 |
| MS.gene054236 | MS.gene60680 | 0.821359 | 4.21E-53 | -1.69E-46 |
| MS.gene054479 | MS.gene60680 | 0.800079 | 1.71E-48 | -1.69E-46 |
| MS.gene054651 | MS.gene60680 | 0.840911 | 6.54E-58 | -1.69E-46 |
| MS.gene054657 | MS.gene60680 | 0.819164 | 1.34E-52 | -1.69E-46 |
| MS.gene05510 | MS.gene60680 | 0.820655 | 6.12E-53 | -1.69E-46 |
| MS.gene05517 | MS.gene60680 | 0.823005 | 1.75E-53 | -1.69E-46 |
| MS.gene055222 | MS.gene60680 | 0.821873 | 3.20E-53 | -1.69E-46 |
| MS.gene055227 | MS.gene60680 | 0.837492 | 5.04E-57 | -1.69E-46 |
| MS.gene055228 | MS.gene60680 | 0.829733 | 4.36E-55 | -1.69E-46 |
| MS.gene055230 | MS.gene60680 | 0.800792 | 1.22E-48 | -1.69E-46 |
| MS.gene055512 | MS.gene60680 | 0.810061 | 1.39E-50 | -1.69E-46 |
| MS.gene055701 | MS.gene60680 | 0.820108 | 8.17E-53 | -1.69E-46 |
| MS.gene055818 | MS.gene60680 | 0.816112 | 6.54E-52 | -1.69E-46 |
| MS.gene055841 | MS.gene60680 | 0.812957 | 3.26E-51 | -1.69E-46 |
| MS.gene055926 | MS.gene60680 | 0.832083 | 1.16E-55 | -1.69E-46 |
| MS.gene05604 | MS.gene60680 | 0.812308 | 4.53E-51 | -1.69E-46 |
| MS.gene056174 | MS.gene60680 | 0.827365 | 1.63E-54 | -1.69E-46 |
| MS.gene056366 | MS.gene60680 | 0.801197 | 1.01E-48 | -1.69E-46 |
| MS.gene056480 | MS.gene60680 | 0.815168 | 1.06E-51 | -1.69E-46 |
| MS.gene056506 | MS.gene60680 | 0.844268 | 8.40E-59 | -1.69E-46 |
| MS.gene056776 | MS.gene60680 | 0.833202 | 6.11E-56 | -1.69E-46 |
| MS.gene05686 | MS.gene60680 | 0.803155 | 3.99E-49 | -1.69E-46 |
| MS.gene056973 | MS.gene60680 | 0.804504 | 2.09E-49 | -1.69E-46 |
| MS.gene057034 | MS.gene60680 | 0.81334 | 2.69E-51 | -1.69E-46 |
| MS.gene057060 | MS.gene60680 | 0.80127 | 9.75E-49 | -1.69E-46 |
| MS.gene057208 | MS.gene60680 | 0.841843 | 3.72E-58 | -1.69E-46 |
| MS.gene057444 | MS.gene60680 | 0.845605 | 3.66E-59 | -1.69E-46 |
| MS.gene05761 | MS.gene60680 | 0.809879 | 1.52E-50 | -1.69E-46 |
| MS.gene057693 | MS.gene60680 | 0.803763 | 2.98E-49 | -1.69E-46 |
| MS.gene057855 | MS.gene60680 | 0.80516 | 1.53E-49 | -1.69E-46 |
| MS.gene057869 | MS.gene60680 | 0.830033 | 3.69E-55 | -1.69E-46 |
| MS.gene057915 | MS.gene60680 | 0.804467 | 2.13E-49 | -1.69E-46 |
| MS.gene058008 | MS.gene60680 | 0.815376 | 9.54E-52 | -1.69E-46 |
| MS.gene058009 | MS.gene60680 | 0.840257 | 9.69E-58 | -1.69E-46 |
| MS.gene058010 | MS.gene60680 | 0.834867 | 2.34E-56 | -1.69E-46 |
| MS.gene058146 | MS.gene60680 | 0.822375 | 2.45E-53 | -1.69E-46 |
| MS.gene058548 | MS.gene60680 | 0.815948 | 7.12E-52 | -1.69E-46 |
| MS.gene058595 | MS.gene60680 | 0.818097 | 2.34E-52 | -1.69E-46 |
| MS.gene058783 | MS.gene60680 | 0.802335 | 5.89E-49 | -1.69E-46 |
| MS.gene058979 | MS.gene60680 | 0.853351 | 2.53E-61 | -1.69E-46 |
| MS.gene059161 | MS.gene60680 | 0.8353 | 1.82E-56 | -1.69E-46 |
| MS.gene059283 | MS.gene60680 | 0.802327 | 5.91E-49 | -1.69E-46 |
| MS.gene05979 | MS.gene60680 | 0.807425 | 5.09E-50 | -1.69E-46 |
| MS.gene059842 | MS.gene60680 | 0.83249 | 9.18E-56 | -1.69E-46 |
| MS.gene060011 | MS.gene60680 | 0.846313 | 2.35E-59 | -1.69E-46 |
| MS.gene060167 | MS.gene60680 | 0.811435 | 7.01E-51 | -1.69E-46 |
| MS.gene060310 | MS.gene60680 | 0.844337 | 8.05E-59 | -1.69E-46 |
| MS.gene060322 | MS.gene60680 | 0.842749 | 2.14E-58 | -1.69E-46 |
| MS.gene060373 | MS.gene60680 | 0.807452 | 5.02E-50 | -1.69E-46 |
| MS.gene06052 | MS.gene60680 | 0.843961 | 1.02E-58 | -1.69E-46 |
| MS.gene060683 | MS.gene60680 | 0.807812 | 4.21E-50 | -1.69E-46 |
| MS.gene060685 | MS.gene60680 | 0.808114 | 3.63E-50 | -1.69E-46 |
| MS.gene060802 | MS.gene60680 | 0.849079 | 4.07E-60 | -1.69E-46 |
| MS.gene060803 | MS.gene60680 | 0.820994 | 5.11E-53 | -1.69E-46 |
| MS.gene060840 | MS.gene60680 | 0.808383 | 3.18E-50 | -1.69E-46 |
| MS.gene060932 | MS.gene60680 | 0.809945 | 1.47E-50 | -1.69E-46 |
| MS.gene060998 | MS.gene60680 | 0.805288 | 1.44E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60680.t1 | MTR_1g098920 | 97.556 | 450 | 11 | 0 | 1 | 450 | 1 | 450 | 0.0 | 913 |
| MS.gene60680.t1 | MTR_6g035315 | 71.194 | 427 | 112 | 5 | 24 | 445 | 22 | 442 | 0.0 | 539 |
| MS.gene60680.t1 | MTR_2g098080 | 47.568 | 370 | 155 | 9 | 89 | 450 | 79 | 417 | 7.22e-95 | 293 |
| MS.gene60680.t1 | MTR_7g103390 | 45.600 | 250 | 108 | 7 | 10 | 252 | 1 | 229 | 3.40e-58 | 198 |
| MS.gene60680.t1 | MTR_1g061640 | 58.642 | 162 | 59 | 3 | 92 | 252 | 95 | 249 | 7.46e-55 | 190 |
| MS.gene60680.t1 | MTR_1g061640 | 48.193 | 83 | 43 | 0 | 365 | 447 | 400 | 482 | 3.35e-19 | 90.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60680.t1 | AT1G21200 | 58.261 | 460 | 160 | 11 | 1 | 447 | 1 | 441 | 2.67e-162 | 465 |
| MS.gene60680.t1 | AT1G21200 | 58.261 | 460 | 160 | 11 | 1 | 447 | 1 | 441 | 2.67e-162 | 465 |
| MS.gene60680.t1 | AT1G76870 | 51.294 | 425 | 137 | 13 | 26 | 445 | 22 | 381 | 1.48e-117 | 349 |
| MS.gene60680.t1 | AT1G76870 | 51.294 | 425 | 137 | 13 | 26 | 445 | 22 | 381 | 1.48e-117 | 349 |
| MS.gene60680.t1 | AT1G76870 | 51.294 | 425 | 137 | 13 | 26 | 445 | 22 | 381 | 1.48e-117 | 349 |
| MS.gene60680.t1 | AT3G10040 | 32.957 | 443 | 217 | 15 | 28 | 447 | 29 | 414 | 4.53e-61 | 205 |
Find 102 sgRNAs with CRISPR-Local
Find 118 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAAGGGAGTAGAGGTAAAA+AGG | 0.185988 | 1.2:+70638756 | MS.gene60680:CDS |
| CAAAAGTATTAGCATGATTT+TGG | 0.192898 | 1.2:-70639422 | None:intergenic |
| TGCAGTTAGCTCTCCGTAAT+AGG | 0.247641 | 1.2:+70639235 | MS.gene60680:CDS |
| CAAAATCATGCTAATACTTT+TGG | 0.261782 | 1.2:+70639423 | MS.gene60680:CDS |
| AGTATTTCAATGACAGATTA+TGG | 0.262386 | 1.2:+70638655 | MS.gene60680:CDS |
| TGATTTACCTGGATCAATTC+AGG | 0.278938 | 1.2:+70638501 | MS.gene60680:CDS |
| GTTCTTACCATAATTGTAAT+AGG | 0.279481 | 1.2:+70639175 | MS.gene60680:CDS |
| ATTACAGCTGTTTCTTATAT+CGG | 0.281358 | 1.2:+70638835 | MS.gene60680:CDS |
| CGTGGTGAGGGGCGGTTAAA+TGG | 0.283530 | 1.2:+70639369 | MS.gene60680:CDS |
| AATGCAGCCTATTACAATTA+TGG | 0.286210 | 1.2:-70639182 | None:intergenic |
| AGAGCAAAAGTTACAAATTC+AGG | 0.286726 | 1.2:+70639596 | MS.gene60680:CDS |
| AATTCAATGATCTTAATAAA+AGG | 0.306147 | 1.2:+70638998 | MS.gene60680:CDS |
| CTCGTATCAGCGTGGACAAA+TGG | 0.372153 | 1.2:+70639482 | MS.gene60680:CDS |
| CAGATTATGGTAAGGGAGAA+AGG | 0.389421 | 1.2:+70638668 | MS.gene60680:CDS |
| AGGGTTGTCGCTACATTCAT+TGG | 0.413758 | 1.2:+70638582 | MS.gene60680:CDS |
| TTTGGTAGCTTTGATTTACC+TGG | 0.427242 | 1.2:+70638490 | MS.gene60680:CDS |
| GGTGTTGGTGGTAATTCTTT+TGG | 0.433044 | 1.2:+70638472 | MS.gene60680:CDS |
| TGCATTGAAGATTGCAATGC+AGG | 0.434565 | 1.2:-70639216 | None:intergenic |
| TATCATGATCATCCCTATTA+CGG | 0.438626 | 1.2:-70639248 | None:intergenic |
| AGATTATGGTAAGGGAGAAA+GGG | 0.443869 | 1.2:+70638669 | MS.gene60680:CDS |
| TCGTATCAGCGTGGACAAAT+GGG | 0.448709 | 1.2:+70639483 | MS.gene60680:CDS |
| GCAGTTAGCTCTCCGTAATA+GGG | 0.450748 | 1.2:+70639236 | MS.gene60680:CDS |
| GGGAACTTCTTGTGAGGTTG+TGG | 0.453879 | 1.2:+70639050 | MS.gene60680:CDS |
| TGTGAAGTGGACTGATAATA+TGG | 0.455278 | 1.2:+70638801 | MS.gene60680:CDS |
| TCTGATGGTGTTGGTGGAAG+TGG | 0.459755 | 1.2:+70638871 | MS.gene60680:CDS |
| ACAAATTCAGGCTGAGAAGT+TGG | 0.463771 | 1.2:+70639608 | MS.gene60680:CDS |
| AATTGTGCTTCCCGTGGTGA+GGG | 0.470364 | 1.2:+70639357 | MS.gene60680:CDS |
| TTTGCTCTTCTAACTGAACT+TGG | 0.476208 | 1.2:-70639581 | None:intergenic |
| AAAAGCTTAATGATATGTTA+GGG | 0.479717 | 1.2:+70639025 | MS.gene60680:CDS |
| TTCAATGACAGATTATGGTA+AGG | 0.481181 | 1.2:+70638660 | MS.gene60680:CDS |
| CTTCATCATGATCATCAAGC+TGG | 0.494557 | 1.2:-70639290 | None:intergenic |
| GCAGAGATTTAGCAAGCAAA+AGG | 0.497660 | 1.2:+70639656 | MS.gene60680:CDS |
| CAAGCAAAAGGACCGGGAGT+TGG | 0.497817 | 1.2:+70639668 | MS.gene60680:CDS |
| CGCCTTGGCAGCGTGTGAAG+TGG | 0.500864 | 1.2:+70638788 | MS.gene60680:CDS |
| TCACATGGAGGAATGATTCA+AGG | 0.506693 | 1.2:+70638448 | MS.gene60680:CDS |
| TTATCTTTCCGAGAAAGAAA+AGG | 0.509656 | 1.2:+70639101 | MS.gene60680:CDS |
| GGCTTTAGAACTGAAGCAAA+AGG | 0.514387 | 1.2:+70639740 | MS.gene60680:CDS |
| CTACATTCATTGGTGCAAGA+TGG | 0.514595 | 1.2:+70638592 | MS.gene60680:CDS |
| GATGGCTTTCCACTCACAAT+GGG | 0.519217 | 1.2:+70638610 | MS.gene60680:CDS |
| AAAAGGGATCGTCGTCGCCT+TGG | 0.519575 | 1.2:+70638773 | MS.gene60680:CDS |
| TCAATGACAGATTATGGTAA+GGG | 0.522539 | 1.2:+70638661 | MS.gene60680:CDS |
| GAAGCTTGAAAATGAACGTA+TGG | 0.528051 | 1.2:+70639719 | MS.gene60680:CDS |
| CAGCATGGTTACAGAAGCAA+TGG | 0.529621 | 1.2:+70639547 | MS.gene60680:CDS |
| CTTAATGATATGTTAGGGAG+GGG | 0.532863 | 1.2:+70639030 | MS.gene60680:CDS |
| GATTTAGCAAGCAAAAGGAC+CGG | 0.534224 | 1.2:+70639661 | MS.gene60680:CDS |
| ATTTAACCGCCCCTCACCAC+GGG | 0.535675 | 1.2:-70639367 | None:intergenic |
| AAACCATCCTCCATAAAACT+AGG | 0.540622 | 1.2:-70638721 | None:intergenic |
| GGACAAATGGGGCAGTCTGA+TGG | 0.540710 | 1.2:+70639495 | MS.gene60680:CDS |
| AAGTGGAAGAAGGAAATTTG+CGG | 0.541346 | 1.2:+70638888 | MS.gene60680:CDS |
| TTTAAAAGCATCGTGTTCAT+CGG | 0.543314 | 1.2:-70639331 | None:intergenic |
| AATGAGTCTCGGCTTTAACT+AGG | 0.543954 | 1.2:+70639764 | MS.gene60680:CDS |
| GACTCAGCTTCTCCAACTCC+CGG | 0.544178 | 1.2:-70639680 | None:intergenic |
| GGAATGATTCAAGGTGGTGT+TGG | 0.544874 | 1.2:+70638457 | MS.gene60680:CDS |
| TAAATTCCATCTAAAAGCGC+AGG | 0.553826 | 1.2:-70639078 | None:intergenic |
| GCTTAATGATATGTTAGGGA+GGG | 0.553961 | 1.2:+70639029 | MS.gene60680:CDS |
| AGAACTTCTGATGGTGTTGG+TGG | 0.554130 | 1.2:+70638865 | MS.gene60680:CDS |
| ATTAGCTTGATGTTGGTGAA+TGG | 0.556372 | 1.2:-70638551 | None:intergenic |
| GACGCGGATTAGCTTGATGT+TGG | 0.561054 | 1.2:-70638558 | None:intergenic |
| CAATTGTGCTTCCCGTGGTG+AGG | 0.561935 | 1.2:+70639356 | MS.gene60680:CDS |
| TTCATCATGATCATCAAGCT+GGG | 0.564029 | 1.2:-70639289 | None:intergenic |
| CATCAAGCTAATCCGCGTCA+AGG | 0.564490 | 1.2:+70638562 | MS.gene60680:CDS |
| ATGATTCAAGGTGGTGTTGG+TGG | 0.565750 | 1.2:+70638460 | MS.gene60680:CDS |
| AGCTTAATGATATGTTAGGG+AGG | 0.573431 | 1.2:+70639028 | MS.gene60680:CDS |
| ATCAAGCTAATCCGCGTCAA+GGG | 0.574042 | 1.2:+70638563 | MS.gene60680:CDS |
| AGGGAGGGGAACTTCTTGTG+AGG | 0.575992 | 1.2:+70639044 | MS.gene60680:CDS |
| AAGCAAAAGGAAATGAGTCT+CGG | 0.578972 | 1.2:+70639753 | MS.gene60680:CDS |
| TGTCAAGATTATTTCAACAA+AGG | 0.579146 | 1.2:+70639459 | MS.gene60680:CDS |
| CTCTGATCACAGCTCTGCAA+AGG | 0.583764 | 1.2:-70638634 | None:intergenic |
| ATTTAGCAAGCAAAAGGACC+GGG | 0.584138 | 1.2:+70639662 | MS.gene60680:CDS |
| GTGCTTCCCGTGGTGAGGGG+CGG | 0.584219 | 1.2:+70639361 | MS.gene60680:CDS |
| GTCCACTTCACACGCTGCCA+AGG | 0.587195 | 1.2:-70638790 | None:intergenic |
| GTGTTGGTGGAAGTGGAAGA+AGG | 0.588046 | 1.2:+70638878 | MS.gene60680:CDS |
| AGATGGCTTTCCACTCACAA+TGG | 0.589107 | 1.2:+70638609 | MS.gene60680:CDS |
| TGATGTTGGTGAATGGTATG+AGG | 0.591910 | 1.2:-70638544 | None:intergenic |
| TGATGAACCTGAATTGATCC+AGG | 0.599004 | 1.2:-70638508 | None:intergenic |
| ATTGTGCTTCCCGTGGTGAG+GGG | 0.600048 | 1.2:+70639358 | MS.gene60680:CDS |
| GGTCAACAGGAAGGGAGTAG+AGG | 0.601547 | 1.2:+70638748 | MS.gene60680:CDS |
| TAGAGAAACAAAGATTCAAG+TGG | 0.601786 | 1.2:+70639634 | MS.gene60680:CDS |
| AAAGACAATTGTGCTTCCCG+TGG | 0.602217 | 1.2:+70639351 | MS.gene60680:CDS |
| TGCAAAGGTCCCATTGTGAG+TGG | 0.608379 | 1.2:-70638619 | None:intergenic |
| GATTATGGTAAGGGAGAAAG+GGG | 0.609744 | 1.2:+70638670 | MS.gene60680:CDS |
| AGCTGTTTCTTATATCGGTG+AGG | 0.609938 | 1.2:+70638840 | MS.gene60680:CDS |
| CATTTAACCGCCCCTCACCA+CGG | 0.615478 | 1.2:-70639368 | None:intergenic |
| CGTATCAGCGTGGACAAATG+GGG | 0.619035 | 1.2:+70639484 | MS.gene60680:CDS |
| GTTGGTGAATGGTATGAGGA+TGG | 0.620557 | 1.2:-70638540 | None:intergenic |
| GATTGCAATGCAGGATCATG+TGG | 0.628342 | 1.2:-70639207 | None:intergenic |
| GGTGAGGATAGAACTTCTGA+TGG | 0.630725 | 1.2:+70638856 | MS.gene60680:CDS |
| AGCATGGTTACAGAAGCAAT+GGG | 0.632029 | 1.2:+70639548 | MS.gene60680:CDS |
| TCGAAAGTCATGGCTGAAAG+AGG | 0.632554 | 1.2:+70638943 | MS.gene60680:CDS |
| GATAGAACTTCTGATGGTGT+TGG | 0.639977 | 1.2:+70638862 | MS.gene60680:CDS |
| AAGAAATTGAAACAAAACCA+AGG | 0.641410 | 1.2:+70639399 | MS.gene60680:CDS |
| TGTTTCGCCGCAGCAATGTG+AGG | 0.648167 | 1.2:+70638972 | MS.gene60680:CDS |
| AATTTATCCTCACATTGCTG+CGG | 0.655462 | 1.2:-70638979 | None:intergenic |
| CTGAAAACATGAGAGCAGCA+TGG | 0.667038 | 1.2:+70639532 | MS.gene60680:CDS |
| AACAAAGGCTCGTATCAGCG+TGG | 0.694218 | 1.2:+70639474 | MS.gene60680:CDS |
| ATGGATGTGAATTTGTCACA+TGG | 0.694479 | 1.2:+70638433 | MS.gene60680:CDS |
| TGTAGCGACAACCCTTGACG+CGG | 0.703142 | 1.2:-70638574 | None:intergenic |
| ATTATGGTAAGGGAGAAAGG+GGG | 0.719074 | 1.2:+70638671 | MS.gene60680:CDS |
| GAGATGTGTGTCGAAAGTCA+TGG | 0.729050 | 1.2:+70638933 | MS.gene60680:CDS |
| CATGGAGGAATGATTCAAGG+TGG | 0.747211 | 1.2:+70638451 | MS.gene60680:CDS |
| AGTGGACTGATAATATGGTG+AGG | 0.762323 | 1.2:+70638806 | MS.gene60680:CDS |
| GATGTGAATTTGTCACATGG+AGG | 0.770898 | 1.2:+70638436 | MS.gene60680:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTCAATGATCTTAATAAA+AGG | + | chr1.2:70638998-70639017 | MS.gene60680:CDS | 15.0% |
| !! | AAAAAGCTTAATGATATGTT+AGG | + | chr1.2:70639024-70639043 | MS.gene60680:CDS | 20.0% |
| !! | AAAAGCTTAATGATATGTTA+GGG | + | chr1.2:70639025-70639044 | MS.gene60680:CDS | 20.0% |
| ! | AAGAAATTGAAACAAAACCA+AGG | + | chr1.2:70639399-70639418 | MS.gene60680:CDS | 25.0% |
| ! | AGTATTTCAATGACAGATTA+TGG | + | chr1.2:70638655-70638674 | MS.gene60680:CDS | 25.0% |
| ! | GTTCTTACCATAATTGTAAT+AGG | + | chr1.2:70639175-70639194 | MS.gene60680:CDS | 25.0% |
| ! | TGTCAAGATTATTTCAACAA+AGG | + | chr1.2:70639459-70639478 | MS.gene60680:CDS | 25.0% |
| !! | CAAAATCATGCTAATACTTT+TGG | + | chr1.2:70639423-70639442 | MS.gene60680:CDS | 25.0% |
| !!! | ATTACAGCTGTTTCTTATAT+CGG | + | chr1.2:70638835-70638854 | MS.gene60680:CDS | 25.0% |
| !!! | CAAAAGTATTAGCATGATTT+TGG | - | chr1.2:70639425-70639444 | None:intergenic | 25.0% |
| AATGCAGCCTATTACAATTA+TGG | - | chr1.2:70639185-70639204 | None:intergenic | 30.0% | |
| AGAGCAAAAGTTACAAATTC+AGG | + | chr1.2:70639596-70639615 | MS.gene60680:CDS | 30.0% | |
| TAGAGAAACAAAGATTCAAG+TGG | + | chr1.2:70639634-70639653 | MS.gene60680:CDS | 30.0% | |
| TATCATGATCATCCCTATTA+CGG | - | chr1.2:70639251-70639270 | None:intergenic | 30.0% | |
| TCAATGACAGATTATGGTAA+GGG | + | chr1.2:70638661-70638680 | MS.gene60680:CDS | 30.0% | |
| TTATCTTTCCGAGAAAGAAA+AGG | + | chr1.2:70639101-70639120 | MS.gene60680:CDS | 30.0% | |
| TTCAATGACAGATTATGGTA+AGG | + | chr1.2:70638660-70638679 | MS.gene60680:CDS | 30.0% | |
| !! | TTTAAAAGCATCGTGTTCAT+CGG | - | chr1.2:70639334-70639353 | None:intergenic | 30.0% |
| AAACCATCCTCCATAAAACT+AGG | - | chr1.2:70638724-70638743 | None:intergenic | 35.0% | |
| AAGCAAAAGGAAATGAGTCT+CGG | + | chr1.2:70639753-70639772 | MS.gene60680:CDS | 35.0% | |
| AAGTGGAAGAAGGAAATTTG+CGG | + | chr1.2:70638888-70638907 | MS.gene60680:CDS | 35.0% | |
| AATTTATCCTCACATTGCTG+CGG | - | chr1.2:70638982-70639001 | None:intergenic | 35.0% | |
| AGATTATGGTAAGGGAGAAA+GGG | + | chr1.2:70638669-70638688 | MS.gene60680:CDS | 35.0% | |
| AGCTTAATGATATGTTAGGG+AGG | + | chr1.2:70639028-70639047 | MS.gene60680:CDS | 35.0% | |
| ATGGATGTGAATTTGTCACA+TGG | + | chr1.2:70638433-70638452 | MS.gene60680:CDS | 35.0% | |
| CTTAATGATATGTTAGGGAG+GGG | + | chr1.2:70639030-70639049 | MS.gene60680:CDS | 35.0% | |
| GAAGCTTGAAAATGAACGTA+TGG | + | chr1.2:70639719-70639738 | MS.gene60680:CDS | 35.0% | |
| GCTTAATGATATGTTAGGGA+GGG | + | chr1.2:70639029-70639048 | MS.gene60680:CDS | 35.0% | |
| TGATTTACCTGGATCAATTC+AGG | + | chr1.2:70638501-70638520 | MS.gene60680:CDS | 35.0% | |
| TTCATCATGATCATCAAGCT+GGG | - | chr1.2:70639292-70639311 | None:intergenic | 35.0% | |
| TTTGCTCTTCTAACTGAACT+TGG | - | chr1.2:70639584-70639603 | None:intergenic | 35.0% | |
| ! | AACTTCGTCCTTTTCTTTCT+CGG | - | chr1.2:70639112-70639131 | None:intergenic | 35.0% |
| ! | ATTAGCTTGATGTTGGTGAA+TGG | - | chr1.2:70638554-70638573 | None:intergenic | 35.0% |
| ! | TAAATTCCATCTAAAAGCGC+AGG | - | chr1.2:70639081-70639100 | None:intergenic | 35.0% |
| !! | TATTAGCATGATTTTGGCCT+TGG | - | chr1.2:70639419-70639438 | None:intergenic | 35.0% |
| !! | TGTGAAGTGGACTGATAATA+TGG | + | chr1.2:70638801-70638820 | MS.gene60680:CDS | 35.0% |
| !! | TTTGGTAGCTTTGATTTACC+TGG | + | chr1.2:70638490-70638509 | MS.gene60680:CDS | 35.0% |
| !!! | TTTATGGAGGATGGTTTTGA+TGG | + | chr1.2:70638727-70638746 | MS.gene60680:CDS | 35.0% |
| ACAAATTCAGGCTGAGAAGT+TGG | + | chr1.2:70639608-70639627 | MS.gene60680:CDS | 40.0% | |
| AGCATGGTTACAGAAGCAAT+GGG | + | chr1.2:70639548-70639567 | MS.gene60680:CDS | 40.0% | |
| ATTATGGTAAGGGAGAAAGG+GGG | + | chr1.2:70638671-70638690 | MS.gene60680:CDS | 40.0% | |
| ATTTAGCAAGCAAAAGGACC+GGG | + | chr1.2:70639662-70639681 | MS.gene60680:CDS | 40.0% | |
| CAGATTATGGTAAGGGAGAA+AGG | + | chr1.2:70638668-70638687 | MS.gene60680:CDS | 40.0% | |
| CTACATTCATTGGTGCAAGA+TGG | + | chr1.2:70638592-70638611 | MS.gene60680:CDS | 40.0% | |
| CTTCATCATGATCATCAAGC+TGG | - | chr1.2:70639293-70639312 | None:intergenic | 40.0% | |
| GAAGGGAGTAGAGGTAAAAA+GGG | + | chr1.2:70638757-70638776 | MS.gene60680:CDS | 40.0% | |
| GATGTGAATTTGTCACATGG+AGG | + | chr1.2:70638436-70638455 | MS.gene60680:CDS | 40.0% | |
| GATTATGGTAAGGGAGAAAG+GGG | + | chr1.2:70638670-70638689 | MS.gene60680:CDS | 40.0% | |
| GATTTAGCAAGCAAAAGGAC+CGG | + | chr1.2:70639661-70639680 | MS.gene60680:CDS | 40.0% | |
| GCAGAGATTTAGCAAGCAAA+AGG | + | chr1.2:70639656-70639675 | MS.gene60680:CDS | 40.0% | |
| GGGGAAAAATTGTACAAGTG+AGG | + | chr1.2:70638690-70638709 | MS.gene60680:CDS | 40.0% | |
| TCACATGGAGGAATGATTCA+AGG | + | chr1.2:70638448-70638467 | MS.gene60680:CDS | 40.0% | |
| TGATGAACCTGAATTGATCC+AGG | - | chr1.2:70638511-70638530 | None:intergenic | 40.0% | |
| TGCATTGAAGATTGCAATGC+AGG | - | chr1.2:70639219-70639238 | None:intergenic | 40.0% | |
| ! | ATTTGCGGTTTTGCAGAAGA+AGG | + | chr1.2:70638903-70638922 | MS.gene60680:CDS | 40.0% |
| ! | GAAAATCCTGCGCTTTTAGA+TGG | + | chr1.2:70639072-70639091 | MS.gene60680:CDS | 40.0% |
| ! | GATAGAACTTCTGATGGTGT+TGG | + | chr1.2:70638862-70638881 | MS.gene60680:CDS | 40.0% |
| ! | TGATGTTGGTGAATGGTATG+AGG | - | chr1.2:70638547-70638566 | None:intergenic | 40.0% |
| ! | TTTGCGGTTTTGCAGAAGAA+GGG | + | chr1.2:70638904-70638923 | MS.gene60680:CDS | 40.0% |
| ! | TTTTGCAGAAGAAGGGTAAG+TGG | + | chr1.2:70638911-70638930 | MS.gene60680:CDS | 40.0% |
| !! | AGCTGTTTCTTATATCGGTG+AGG | + | chr1.2:70638840-70638859 | MS.gene60680:CDS | 40.0% |
| !! | AGTGGACTGATAATATGGTG+AGG | + | chr1.2:70638806-70638825 | MS.gene60680:CDS | 40.0% |
| !! | GGCTTTAGAACTGAAGCAAA+AGG | + | chr1.2:70639740-70639759 | MS.gene60680:CDS | 40.0% |
| !! | GTTTTGATGGTCAACAGGAA+GGG | + | chr1.2:70638740-70638759 | MS.gene60680:CDS | 40.0% |
| !!! | GGTGTTGGTGGTAATTCTTT+TGG | + | chr1.2:70638472-70638491 | MS.gene60680:CDS | 40.0% |
| AAAGACAATTGTGCTTCCCG+TGG | + | chr1.2:70639351-70639370 | MS.gene60680:CDS | 45.0% | |
| AGATGGCTTTCCACTCACAA+TGG | + | chr1.2:70638609-70638628 | MS.gene60680:CDS | 45.0% | |
| AGGGTTGTCGCTACATTCAT+TGG | + | chr1.2:70638582-70638601 | MS.gene60680:CDS | 45.0% | |
| ATCAAGCTAATCCGCGTCAA+GGG | + | chr1.2:70638563-70638582 | MS.gene60680:CDS | 45.0% | |
| CAGCATGGTTACAGAAGCAA+TGG | + | chr1.2:70639547-70639566 | MS.gene60680:CDS | 45.0% | |
| CATGGAGGAATGATTCAAGG+TGG | + | chr1.2:70638451-70638470 | MS.gene60680:CDS | 45.0% | |
| CTGAAAACATGAGAGCAGCA+TGG | + | chr1.2:70639532-70639551 | MS.gene60680:CDS | 45.0% | |
| GAGATGTGTGTCGAAAGTCA+TGG | + | chr1.2:70638933-70638952 | MS.gene60680:CDS | 45.0% | |
| GATGGCTTTCCACTCACAAT+GGG | + | chr1.2:70638610-70638629 | MS.gene60680:CDS | 45.0% | |
| GATTGCAATGCAGGATCATG+TGG | - | chr1.2:70639210-70639229 | None:intergenic | 45.0% | |
| GGAAGGGAGTAGAGGTAAAA+AGG | + | chr1.2:70638756-70638775 | MS.gene60680:CDS | 45.0% | |
| GGAATGATTCAAGGTGGTGT+TGG | + | chr1.2:70638457-70638476 | MS.gene60680:CDS | 45.0% | |
| GGTGAGGATAGAACTTCTGA+TGG | + | chr1.2:70638856-70638875 | MS.gene60680:CDS | 45.0% | |
| TCGAAAGTCATGGCTGAAAG+AGG | + | chr1.2:70638943-70638962 | MS.gene60680:CDS | 45.0% | |
| TCGTATCAGCGTGGACAAAT+GGG | + | chr1.2:70639483-70639502 | MS.gene60680:CDS | 45.0% | |
| ! | AGAACTTCTGATGGTGTTGG+TGG | + | chr1.2:70638865-70638884 | MS.gene60680:CDS | 45.0% |
| ! | ATGATTCAAGGTGGTGTTGG+TGG | + | chr1.2:70638460-70638479 | MS.gene60680:CDS | 45.0% |
| ! | CATGCTGCTCTCATGTTTTC+AGG | - | chr1.2:70639534-70639553 | None:intergenic | 45.0% |
| ! | GCAGTTAGCTCTCCGTAATA+GGG | + | chr1.2:70639236-70639255 | MS.gene60680:CDS | 45.0% |
| ! | GTTGGTGAATGGTATGAGGA+TGG | - | chr1.2:70638543-70638562 | None:intergenic | 45.0% |
| ! | TGCAGTTAGCTCTCCGTAAT+AGG | + | chr1.2:70639235-70639254 | MS.gene60680:CDS | 45.0% |
| !! | GGTTTTGATGGTCAACAGGA+AGG | + | chr1.2:70638739-70638758 | MS.gene60680:CDS | 45.0% |
| !!! | AGACGAGCCTAGTTTTATGG+AGG | + | chr1.2:70638714-70638733 | MS.gene60680:CDS | 45.0% |
| !!! | GAGCCTAGTTTTATGGAGGA+TGG | + | chr1.2:70638718-70638737 | MS.gene60680:CDS | 45.0% |
| !!! | GGAAGACGAGCCTAGTTTTA+TGG | + | chr1.2:70638711-70638730 | MS.gene60680:CDS | 45.0% |
| !!! | GGATGGTTTTGATGGTCAAC+AGG | + | chr1.2:70638735-70638754 | MS.gene60680:CDS | 45.0% |
| AACAAAGGCTCGTATCAGCG+TGG | + | chr1.2:70639474-70639493 | MS.gene60680:CDS | 50.0% | |
| AATTGTGCTTCCCGTGGTGA+GGG | + | chr1.2:70639357-70639376 | MS.gene60680:CDS | 50.0% | |
| CATCAAGCTAATCCGCGTCA+AGG | + | chr1.2:70638562-70638581 | MS.gene60680:CDS | 50.0% | |
| CGTATCAGCGTGGACAAATG+GGG | + | chr1.2:70639484-70639503 | MS.gene60680:CDS | 50.0% | |
| CTCGTATCAGCGTGGACAAA+TGG | + | chr1.2:70639482-70639501 | MS.gene60680:CDS | 50.0% | |
| CTCTGATCACAGCTCTGCAA+AGG | - | chr1.2:70638637-70638656 | None:intergenic | 50.0% | |
| GGGAACTTCTTGTGAGGTTG+TGG | + | chr1.2:70639050-70639069 | MS.gene60680:CDS | 50.0% | |
| TGCAAAGGTCCCATTGTGAG+TGG | - | chr1.2:70638622-70638641 | None:intergenic | 50.0% | |
| ! | GTGTTGGTGGAAGTGGAAGA+AGG | + | chr1.2:70638878-70638897 | MS.gene60680:CDS | 50.0% |
| !! | GACGCGGATTAGCTTGATGT+TGG | - | chr1.2:70638561-70638580 | None:intergenic | 50.0% |
| !! | TCTGATGGTGTTGGTGGAAG+TGG | + | chr1.2:70638871-70638890 | MS.gene60680:CDS | 50.0% |
| AAAAGGGATCGTCGTCGCCT+TGG | + | chr1.2:70638773-70638792 | MS.gene60680:CDS | 55.0% | |
| AGGGAGGGGAACTTCTTGTG+AGG | + | chr1.2:70639044-70639063 | MS.gene60680:CDS | 55.0% | |
| ATTGTGCTTCCCGTGGTGAG+GGG | + | chr1.2:70639358-70639377 | MS.gene60680:CDS | 55.0% | |
| ATTTAACCGCCCCTCACCAC+GGG | - | chr1.2:70639370-70639389 | None:intergenic | 55.0% | |
| CAAGCAAAAGGACCGGGAGT+TGG | + | chr1.2:70639668-70639687 | MS.gene60680:CDS | 55.0% | |
| CAATTGTGCTTCCCGTGGTG+AGG | + | chr1.2:70639356-70639375 | MS.gene60680:CDS | 55.0% | |
| CATTTAACCGCCCCTCACCA+CGG | - | chr1.2:70639371-70639390 | None:intergenic | 55.0% | |
| GACTCAGCTTCTCCAACTCC+CGG | - | chr1.2:70639683-70639702 | None:intergenic | 55.0% | |
| GGACAAATGGGGCAGTCTGA+TGG | + | chr1.2:70639495-70639514 | MS.gene60680:CDS | 55.0% | |
| GGTCAACAGGAAGGGAGTAG+AGG | + | chr1.2:70638748-70638767 | MS.gene60680:CDS | 55.0% | |
| TGTAGCGACAACCCTTGACG+CGG | - | chr1.2:70638577-70638596 | None:intergenic | 55.0% | |
| TGTTTCGCCGCAGCAATGTG+AGG | + | chr1.2:70638972-70638991 | MS.gene60680:CDS | 55.0% | |
| CGTGGTGAGGGGCGGTTAAA+TGG | + | chr1.2:70639369-70639388 | MS.gene60680:CDS | 60.0% | |
| GTCCACTTCACACGCTGCCA+AGG | - | chr1.2:70638793-70638812 | None:intergenic | 60.0% | |
| CGCCTTGGCAGCGTGTGAAG+TGG | + | chr1.2:70638788-70638807 | MS.gene60680:CDS | 65.0% | |
| GTGCTTCCCGTGGTGAGGGG+CGG | + | chr1.2:70639361-70639380 | MS.gene60680:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 70638433 | 70639785 | 70638433 | ID=MS.gene60680 |
| chr1.2 | mRNA | 70638433 | 70639785 | 70638433 | ID=MS.gene60680.t1;Parent=MS.gene60680 |
| chr1.2 | exon | 70638433 | 70639785 | 70638433 | ID=MS.gene60680.t1.exon1;Parent=MS.gene60680.t1 |
| chr1.2 | CDS | 70638433 | 70639785 | 70638433 | ID=cds.MS.gene60680.t1;Parent=MS.gene60680.t1 |
| Gene Sequence |
| Protein sequence |
