AlfalfaGEDB | MS.gene60357

M. sativa cultivar XinJiangDaYe / MS.gene60357

KEGG: K10881
GO: GO:0005575 GO:0006405 GO:0006406 GO:0006810 GO:0006913 GO:0008150 GO:0008541 GO:0009987 GO:0015931 GO:0016043 GO:0022607 GO:0032991 GO:0034622 GO:0043248 GO:0043933 GO:0046907 GO:0050657 GO:0050658 GO:0051028 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051641 GO:0065003 GO:0071702 GO:0071705 GO:0071840
PFAM: PF05160.16

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60357.t1	GAU19652.1	91.2	34	3	0	1	34	1	34	3.40E-07	63.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60357.t1	Q9FL96	77.8	36	7	1	1	35	1	36	5.5e-06	51.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60357.t1	A0A2Z6LNG4	91.2	34	3	0	1	34	1	34	2.5e-07	63.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049254	MS.gene60357	0.854456	1.22E-61	-1.69E-46
MS.gene049293	MS.gene60357	-0.806234	9.08E-50	-1.69E-46
MS.gene049404	MS.gene60357	0.835179	1.95E-56	-1.69E-46
MS.gene049499	MS.gene60357	0.813086	3.06E-51	-1.69E-46
MS.gene049711	MS.gene60357	0.805676	1.19E-49	-1.69E-46
MS.gene049821	MS.gene60357	0.803027	4.24E-49	-1.69E-46
MS.gene049824	MS.gene60357	0.80559	1.24E-49	-1.69E-46
MS.gene049825	MS.gene60357	0.83735	5.47E-57	-1.69E-46
MS.gene049873	MS.gene60357	0.825927	3.59E-54	-1.69E-46
MS.gene050026	MS.gene60357	0.818033	2.42E-52	-1.69E-46
MS.gene050273	MS.gene60357	0.800653	1.30E-48	-1.69E-46
MS.gene050559	MS.gene60357	0.84438	7.83E-59	-1.69E-46
MS.gene050561	MS.gene60357	0.834042	3.77E-56	-1.69E-46
MS.gene050562	MS.gene60357	0.826167	3.15E-54	-1.69E-46
MS.gene051247	MS.gene60357	0.821432	4.05E-53	-1.69E-46
MS.gene051279	MS.gene60357	0.83942	1.60E-57	-1.69E-46
MS.gene051332	MS.gene60357	0.810671	1.03E-50	-1.69E-46
MS.gene051574	MS.gene60357	0.820376	7.09E-53	-1.69E-46
MS.gene051594	MS.gene60357	0.819007	1.46E-52	-1.69E-46
MS.gene051676	MS.gene60357	0.805965	1.03E-49	-1.69E-46
MS.gene051744	MS.gene60357	0.835448	1.67E-56	-1.69E-46
MS.gene051832	MS.gene60357	0.807507	4.89E-50	-1.69E-46
MS.gene051957	MS.gene60357	0.802524	5.38E-49	-1.69E-46
MS.gene051975	MS.gene60357	0.802325	5.92E-49	-1.69E-46
MS.gene052108	MS.gene60357	0.814284	1.67E-51	-1.69E-46
MS.gene052208	MS.gene60357	0.800148	1.65E-48	-1.69E-46
MS.gene052239	MS.gene60357	-0.82362	1.26E-53	-1.69E-46
MS.gene052519	MS.gene60357	0.830797	2.40E-55	-1.69E-46
MS.gene052534	MS.gene60357	0.803016	4.26E-49	-1.69E-46
MS.gene052582	MS.gene60357	0.857453	1.62E-62	-1.69E-46
MS.gene052584	MS.gene60357	0.854301	1.35E-61	-1.69E-46
MS.gene052586	MS.gene60357	0.801177	1.02E-48	-1.69E-46
MS.gene052773	MS.gene60357	0.811386	7.18E-51	-1.69E-46
MS.gene053006	MS.gene60357	0.853214	2.77E-61	-1.69E-46
MS.gene053226	MS.gene60357	0.837844	4.09E-57	-1.69E-46
MS.gene05365	MS.gene60357	0.821892	3.17E-53	-1.69E-46
MS.gene053931	MS.gene60357	0.816761	4.68E-52	-1.69E-46
MS.gene053995	MS.gene60357	0.816251	6.09E-52	-1.69E-46
MS.gene05415	MS.gene60357	0.821528	3.85E-53	-1.69E-46
MS.gene054222	MS.gene60357	0.811288	7.54E-51	-1.69E-46
MS.gene05423	MS.gene60357	0.80526	1.45E-49	-1.69E-46
MS.gene054291	MS.gene60357	-0.804414	2.19E-49	-1.69E-46
MS.gene054576	MS.gene60357	0.815923	7.21E-52	-1.69E-46
MS.gene054651	MS.gene60357	0.859305	4.53E-63	-1.69E-46
MS.gene054775	MS.gene60357	0.817954	2.52E-52	-1.69E-46
MS.gene054991	MS.gene60357	0.80035	1.50E-48	-1.69E-46
MS.gene055228	MS.gene60357	0.80643	8.26E-50	-1.69E-46
MS.gene05548	MS.gene60357	0.813402	2.61E-51	-1.69E-46
MS.gene055586	MS.gene60357	0.831048	2.08E-55	-1.69E-46
MS.gene055589	MS.gene60357	0.800035	1.74E-48	-1.69E-46
MS.gene055778	MS.gene60357	0.815378	9.53E-52	-1.69E-46
MS.gene055808	MS.gene60357	0.814404	1.57E-51	-1.69E-46
MS.gene055818	MS.gene60357	0.822625	2.14E-53	-1.69E-46
MS.gene055841	MS.gene60357	0.866121	3.59E-65	-1.69E-46
MS.gene056050	MS.gene60357	0.804399	2.20E-49	-1.69E-46
MS.gene056091	MS.gene60357	0.815356	9.64E-52	-1.69E-46
MS.gene056162	MS.gene60357	0.804459	2.14E-49	-1.69E-46
MS.gene056366	MS.gene60357	0.837668	4.54E-57	-1.69E-46
MS.gene056498	MS.gene60357	0.805894	1.07E-49	-1.69E-46
MS.gene056776	MS.gene60357	0.833393	5.47E-56	-1.69E-46
MS.gene05686	MS.gene60357	0.825925	3.59E-54	-1.69E-46
MS.gene057026	MS.gene60357	0.805372	1.38E-49	-1.69E-46
MS.gene057060	MS.gene60357	0.833514	5.11E-56	-1.69E-46
MS.gene057102	MS.gene60357	0.823774	1.16E-53	-1.69E-46
MS.gene057208	MS.gene60357	0.854926	8.89E-62	-1.69E-46
MS.gene057444	MS.gene60357	0.818839	1.59E-52	-1.69E-46
MS.gene057693	MS.gene60357	0.813815	2.11E-51	-1.69E-46
MS.gene057705	MS.gene60357	0.815484	9.03E-52	-1.69E-46
MS.gene058037	MS.gene60357	0.827242	1.74E-54	-1.69E-46
MS.gene058279	MS.gene60357	0.82167	3.57E-53	-1.69E-46
MS.gene058625	MS.gene60357	0.837721	4.40E-57	-1.69E-46
MS.gene058783	MS.gene60357	0.800226	1.59E-48	-1.69E-46
MS.gene058979	MS.gene60357	0.800382	1.48E-48	-1.69E-46
MS.gene05950	MS.gene60357	0.810617	1.05E-50	-1.69E-46
MS.gene05953	MS.gene60357	0.82518	5.39E-54	-1.69E-46
MS.gene05954	MS.gene60357	0.854779	9.80E-62	-1.69E-46
MS.gene059584	MS.gene60357	0.802254	6.12E-49	-1.69E-46
MS.gene059625	MS.gene60357	0.820788	5.70E-53	-1.69E-46
MS.gene05985	MS.gene60357	-0.819353	1.21E-52	-1.69E-46
MS.gene059935	MS.gene60357	0.817478	3.23E-52	-1.69E-46
MS.gene060365	MS.gene60357	0.836693	8.06E-57	-1.69E-46
MS.gene060429	MS.gene60357	0.828509	8.64E-55	-1.69E-46
MS.gene060521	MS.gene60357	0.843611	1.26E-58	-1.69E-46
MS.gene060572	MS.gene60357	0.808798	2.59E-50	-1.69E-46
MS.gene060634	MS.gene60357	-0.828312	9.64E-55	-1.69E-46
MS.gene060935	MS.gene60357	0.805343	1.40E-49	-1.69E-46
MS.gene061040	MS.gene60357	-0.808519	2.98E-50	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene48281	MS.gene60357	PPI
MS.gene28183	MS.gene60357	PPI
MS.gene014485	MS.gene60357	PPI
MS.gene06729	MS.gene60357	PPI
MS.gene60357	MS.gene70283	PPI
MS.gene014393	MS.gene60357	PPI
MS.gene58136	MS.gene60357	PPI
MS.gene062770	MS.gene60357	PPI
MS.gene58134	MS.gene60357	PPI
MS.gene033335	MS.gene60357	PPI
MS.gene025808	MS.gene60357	PPI
MS.gene072220	MS.gene60357	PPI
MS.gene40486	MS.gene60357	PPI
MS.gene06946	MS.gene60357	PPI
MS.gene60357	MS.gene69373	PPI
MS.gene030686	MS.gene60357	PPI
MS.gene59485	MS.gene60357	PPI
MS.gene011376	MS.gene60357	PPI
MS.gene32974	MS.gene60357	PPI
MS.gene044013	MS.gene60357	PPI
MS.gene44230	MS.gene60357	PPI
MS.gene60357	MS.gene21169	PPI
MS.gene011065	MS.gene60357	PPI
MS.gene058061	MS.gene60357	PPI
MS.gene64789	MS.gene60357	PPI
MS.gene051630	MS.gene60357	PPI
MS.gene67460	MS.gene60357	PPI
MS.gene01697	MS.gene60357	PPI
MS.gene24375	MS.gene60357	PPI
MS.gene01284	MS.gene60357	PPI
MS.gene36633	MS.gene60357	PPI
MS.gene67099	MS.gene60357	PPI
MS.gene60357	MS.gene21801	PPI
MS.gene057145	MS.gene60357	PPI
MS.gene28454	MS.gene60357	PPI
MS.gene012127	MS.gene60357	PPI
MS.gene24542	MS.gene60357	PPI
MS.gene09974	MS.gene60357	PPI
MS.gene004328	MS.gene60357	PPI
MS.gene60357	MS.gene36746	PPI
MS.gene48400	MS.gene60357	PPI
MS.gene70282	MS.gene60357	PPI
MS.gene030685	MS.gene60357	PPI
MS.gene42312	MS.gene60357	PPI
MS.gene57218	MS.gene60357	PPI
MS.gene52815	MS.gene60357	PPI
MS.gene072221	MS.gene60357	PPI
MS.gene60357	MS.gene057144	PPI
MS.gene002648	MS.gene60357	PPI
MS.gene60357	MS.gene064786	PPI
MS.gene024828	MS.gene60357	PPI
MS.gene69461	MS.gene60357	PPI
MS.gene064694	MS.gene60357	PPI
MS.gene017622	MS.gene60357	PPI
MS.gene013607	MS.gene60357	PPI
MS.gene89672	MS.gene60357	PPI
MS.gene002306	MS.gene60357	PPI
MS.gene68079	MS.gene60357	PPI
MS.gene02265	MS.gene60357	PPI
MS.gene48279	MS.gene60357	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 17 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGACGTTCTCGGAAGGTTT+TGG	0.276388	8.2:-19241799	None:intergenic
CTCAGCAATGGGAAGATGAT+TGG	0.389575	8.2:+19242054	MS.gene60357:CDS
TCAGCTAAAGAGAGAGTTAG+AGG	0.407568	8.2:+19242109	MS.gene60357:CDS
GAGATCAACCTTGACGTTCT+CGG	0.418361	8.2:-19241809	None:intergenic
AGACAAGGAGGAAGGGAGAG+AGG	0.431303	8.2:+19242028	MS.gene60357:CDS
TGAAGACGACGACGAGTTCG+AGG	0.463513	8.2:+19241834	MS.gene60357:CDS
TCAGCAATGGGAAGATGATT+GGG	0.477084	8.2:+19242055	MS.gene60357:CDS
GGAGAGAGGTAGCTCAGCAA+TGG	0.520450	8.2:+19242042	MS.gene60357:CDS
GGTTGGGAAGACAAGGAGGA+AGG	0.522923	8.2:+19242020	MS.gene60357:intron
GAGAGAGGTAGCTCAGCAAT+GGG	0.525728	8.2:+19242043	MS.gene60357:CDS
GTTGGGAAGACAAGGAGGAA+GGG	0.529450	8.2:+19242021	MS.gene60357:CDS
TCAACCTTGACGTTCTCGGA+AGG	0.583809	8.2:-19241805	None:intergenic
ACTTGCAGGTTGGGAAGACA+AGG	0.596138	8.2:+19242013	MS.gene60357:intron
CAGCTAAAGAGAGAGTTAGA+GGG	0.638489	8.2:+19242110	MS.gene60357:CDS
GAGGAGTTTGAAATCAACGA+AGG	0.657612	8.2:+19241853	MS.gene60357:CDS
TGCAGGTTGGGAAGACAAGG+AGG	0.659645	8.2:+19242016	MS.gene60357:intron
AAAACCTTCCGAGAACGTCA+AGG	0.660047	8.2:+19241801	MS.gene60357:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TACTAACATGAATTCAAATC+TGG	-	chr8.2:19241960-19241979	None:intergenic	25.0%
	AAATTGATTGCAACAACGAT+TGG	-	chr8.2:19241917-19241936	None:intergenic	30.0%
	AACAACGATTGGAATGAAGA+GGG	-	chr8.2:19241906-19241925	None:intergenic	35.0%
!	ATCTTATGTAACTTGCAGGT+TGG	+	chr8.2:19242003-19242022	MS.gene60357:intron	35.0%
!	TCTTATGTAACTTGCAGGTT+GGG	+	chr8.2:19242004-19242023	MS.gene60357:intron	35.0%
!	TGCAATCTTATGTAACTTGC+AGG	+	chr8.2:19241999-19242018	MS.gene60357:intron	35.0%
	AATGAAGAGGGGTTAAAGGT+TGG	-	chr8.2:19241894-19241913	None:intergenic	40.0%
	ACAACGATTGGAATGAAGAG+GGG	-	chr8.2:19241905-19241924	None:intergenic	40.0%
	CAACAACGATTGGAATGAAG+AGG	-	chr8.2:19241907-19241926	None:intergenic	40.0%
	CAGCTAAAGAGAGAGTTAGA+GGG	+	chr8.2:19242110-19242129	MS.gene60357:CDS	40.0%
	TCAGCAATGGGAAGATGATT+GGG	+	chr8.2:19242055-19242074	MS.gene60357:CDS	40.0%
	TCAGCTAAAGAGAGAGTTAG+AGG	+	chr8.2:19242109-19242128	MS.gene60357:CDS	40.0%
	TTGGAATGAAGAGGGGTTAA+AGG	-	chr8.2:19241898-19241917	None:intergenic	40.0%
!	GAGGAGTTTGAAATCAACGA+AGG	+	chr8.2:19241853-19241872	MS.gene60357:CDS	40.0%
	AAAACCTTCCGAGAACGTCA+AGG	+	chr8.2:19241801-19241820	MS.gene60357:CDS	45.0%
	CTCAGCAATGGGAAGATGAT+TGG	+	chr8.2:19242054-19242073	MS.gene60357:CDS	45.0%
	GAGATCAACCTTGACGTTCT+CGG	-	chr8.2:19241812-19241831	None:intergenic	45.0%
!	TTGACGTTCTCGGAAGGTTT+TGG	-	chr8.2:19241802-19241821	None:intergenic	45.0%
	GAGAGAGGTAGCTCAGCAAT+GGG	+	chr8.2:19242043-19242062	MS.gene60357:CDS	50.0%
	GTTGGGAAGACAAGGAGGAA+GGG	+	chr8.2:19242021-19242040	MS.gene60357:CDS	50.0%
	TCAACCTTGACGTTCTCGGA+AGG	-	chr8.2:19241808-19241827	None:intergenic	50.0%
!	ACTTGCAGGTTGGGAAGACA+AGG	+	chr8.2:19242013-19242032	MS.gene60357:intron	50.0%
!!!	GTTCTCGGAAGGTTTTGGCT+CGG	-	chr8.2:19241797-19241816	None:intergenic	50.0%
	AGACAAGGAGGAAGGGAGAG+AGG	+	chr8.2:19242028-19242047	MS.gene60357:CDS	55.0%
	GGAGAGAGGTAGCTCAGCAA+TGG	+	chr8.2:19242042-19242061	MS.gene60357:CDS	55.0%
	GGTTGGGAAGACAAGGAGGA+AGG	+	chr8.2:19242020-19242039	MS.gene60357:intron	55.0%
	TGAAGACGACGACGAGTTCG+AGG	+	chr8.2:19241834-19241853	MS.gene60357:CDS	55.0%
!	TGCAGGTTGGGAAGACAAGG+AGG	+	chr8.2:19242016-19242035	MS.gene60357:intron	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.2	gene	19241787	19242151	19241787	ID=MS.gene60357
chr8.2	mRNA	19241787	19242151	19241787	ID=MS.gene60357.t1;Parent=MS.gene60357
chr8.2	exon	19241787	19241874	19241787	ID=MS.gene60357.t1.exon1;Parent=MS.gene60357.t1
chr8.2	CDS	19241787	19241874	19241787	ID=cds.MS.gene60357.t1;Parent=MS.gene60357.t1
chr8.2	exon	19242021	19242151	19242021	ID=MS.gene60357.t1.exon2;Parent=MS.gene60357.t1
chr8.2	CDS	19242021	19242151	19242021	ID=cds.MS.gene60357.t1;Parent=MS.gene60357.t1

Gene Sequence	>MS.gene60357 ATGGCAACCGAGCCAAAACCTTCCGAGAACGTCAAGGTTGATCTCTTTGAAGACGACGACGAGTTCGAGGAGTTTGAAATCAACGAAGGTAACTTCAAATCGTTCCAACCTTTAACCCCTCTTCATTCCAATCGTTGTTGCAATCAATTTCAGCTTCATTCAATTTCGATCCAGATTTGAATTCATGTTAGTAGTTTCTTATTGTTTATTTTTGCAATCTTATGTAACTTGCAGGTTGGGAAGACAAGGAGGAAGGGAGAGAGGTAGCTCAGCAATGGGAAGATGATTGGGATGATGATGATGTTAGTGATGATTTCTCTCTTCAGCTAAAGAGAGAGTTAGAGGGCAATGCTGAGAAGAAATAA
Protein sequence	>MS.gene60357.t1 MATEPKPSENVKVDLFEDDDEFEEFEINEGWEDKEEGREVAQQWEDDWDDDDVSDDFSLQLKRELEGNAEKK