AlfalfaGEDB | MS.gene48389

M. sativa cultivar XinJiangDaYe / MS.gene48389

KEGG: K10881
GO: GO:0005575 GO:0006405 GO:0006406 GO:0006810 GO:0006913 GO:0008150 GO:0008541 GO:0009987 GO:0015931 GO:0016043 GO:0022607 GO:0032991 GO:0034622 GO:0043248 GO:0043933 GO:0046907 GO:0050657 GO:0050658 GO:0051028 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051641 GO:0065003 GO:0071702 GO:0071705 GO:0071840
PFAM: PF05160.16

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48389.t1	GAU19652.1	91.2	34	3	0	1	34	1	34	3.40E-07	63.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48389.t1	Q9FL96	77.8	36	7	1	1	35	1	36	5.5e-06	51.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48389.t1	A0A2Z6LNG4	91.2	34	3	0	1	34	1	34	2.5e-07	63.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049254	MS.gene48389	0.856018	4.27E-62	-1.69E-46
MS.gene049271	MS.gene48389	0.802338	5.88E-49	-1.69E-46
MS.gene049404	MS.gene48389	0.850238	1.93E-60	-1.69E-46
MS.gene049821	MS.gene48389	0.812454	4.21E-51	-1.69E-46
MS.gene049824	MS.gene48389	0.802078	6.65E-49	-1.69E-46
MS.gene049825	MS.gene48389	0.832567	8.78E-56	-1.69E-46
MS.gene049873	MS.gene48389	0.833994	3.87E-56	-1.69E-46
MS.gene050026	MS.gene48389	0.828061	1.11E-54	-1.69E-46
MS.gene050273	MS.gene48389	0.812616	3.88E-51	-1.69E-46
MS.gene050355	MS.gene48389	0.822758	2.00E-53	-1.69E-46
MS.gene050559	MS.gene48389	0.839445	1.58E-57	-1.69E-46
MS.gene050561	MS.gene48389	0.831643	1.49E-55	-1.69E-46
MS.gene050562	MS.gene48389	0.824392	8.27E-54	-1.69E-46
MS.gene050750	MS.gene48389	0.810334	1.21E-50	-1.69E-46
MS.gene050807	MS.gene48389	0.80939	1.94E-50	-1.69E-46
MS.gene050953	MS.gene48389	0.813542	2.43E-51	-1.69E-46
MS.gene051198	MS.gene48389	0.809277	2.05E-50	-1.69E-46
MS.gene051247	MS.gene48389	0.832075	1.16E-55	-1.69E-46
MS.gene051279	MS.gene48389	0.851579	8.09E-61	-1.69E-46
MS.gene051332	MS.gene48389	0.808119	3.62E-50	-1.69E-46
MS.gene051574	MS.gene48389	0.826077	3.31E-54	-1.69E-46
MS.gene051594	MS.gene48389	0.82082	5.60E-53	-1.69E-46
MS.gene051719	MS.gene48389	0.803969	2.71E-49	-1.69E-46
MS.gene051720	MS.gene48389	0.802911	4.48E-49	-1.69E-46
MS.gene051744	MS.gene48389	0.842294	2.82E-58	-1.69E-46
MS.gene051957	MS.gene48389	0.815262	1.01E-51	-1.69E-46
MS.gene052108	MS.gene48389	0.805021	1.63E-49	-1.69E-46
MS.gene052208	MS.gene48389	0.820189	7.82E-53	-1.69E-46
MS.gene052239	MS.gene48389	-0.825992	3.46E-54	-1.69E-46
MS.gene052519	MS.gene48389	0.831177	1.94E-55	-1.69E-46
MS.gene052582	MS.gene48389	0.868422	6.61E-66	-1.69E-46
MS.gene052584	MS.gene48389	0.864034	1.63E-64	-1.69E-46
MS.gene052586	MS.gene48389	0.820953	5.22E-53	-1.69E-46
MS.gene052773	MS.gene48389	0.811015	8.65E-51	-1.69E-46
MS.gene052867	MS.gene48389	0.810067	1.39E-50	-1.69E-46
MS.gene053006	MS.gene48389	0.862898	3.66E-64	-1.69E-46
MS.gene053054	MS.gene48389	-0.80656	7.75E-50	-1.69E-46
MS.gene053166	MS.gene48389	0.812389	4.34E-51	-1.69E-46
MS.gene053226	MS.gene48389	0.844047	9.63E-59	-1.69E-46
MS.gene05365	MS.gene48389	0.834362	3.13E-56	-1.69E-46
MS.gene053883	MS.gene48389	0.804749	1.86E-49	-1.69E-46
MS.gene053931	MS.gene48389	0.819771	9.75E-53	-1.69E-46
MS.gene053995	MS.gene48389	0.815454	9.17E-52	-1.69E-46
MS.gene05414	MS.gene48389	0.806223	9.13E-50	-1.69E-46
MS.gene05415	MS.gene48389	0.829582	4.75E-55	-1.69E-46
MS.gene05423	MS.gene48389	0.806716	7.19E-50	-1.69E-46
MS.gene054291	MS.gene48389	-0.818721	1.69E-52	-1.69E-46
MS.gene054405	MS.gene48389	0.807137	5.85E-50	-1.69E-46
MS.gene054559	MS.gene48389	0.805009	1.64E-49	-1.69E-46
MS.gene054576	MS.gene48389	0.82026	7.54E-53	-1.69E-46
MS.gene054651	MS.gene48389	0.867942	9.43E-66	-1.69E-46
MS.gene054657	MS.gene48389	0.804824	1.79E-49	-1.69E-46
MS.gene054775	MS.gene48389	0.836659	8.22E-57	-1.69E-46
MS.gene055228	MS.gene48389	0.81656	5.19E-52	-1.69E-46
MS.gene055466	MS.gene48389	0.800547	1.37E-48	-1.69E-46
MS.gene05548	MS.gene48389	0.807154	5.81E-50	-1.69E-46
MS.gene055586	MS.gene48389	0.835891	1.29E-56	-1.69E-46
MS.gene055589	MS.gene48389	0.801417	9.09E-49	-1.69E-46
MS.gene055778	MS.gene48389	0.836027	1.19E-56	-1.69E-46
MS.gene055808	MS.gene48389	0.823132	1.63E-53	-1.69E-46
MS.gene055818	MS.gene48389	0.829253	5.71E-55	-1.69E-46
MS.gene055841	MS.gene48389	0.859219	4.81E-63	-1.69E-46
MS.gene05604	MS.gene48389	0.80219	6.31E-49	-1.69E-46
MS.gene056050	MS.gene48389	0.816033	6.81E-52	-1.69E-46
MS.gene056091	MS.gene48389	0.821056	4.95E-53	-1.69E-46
MS.gene056162	MS.gene48389	0.819304	1.25E-52	-1.69E-46
MS.gene056174	MS.gene48389	0.810831	9.48E-51	-1.69E-46
MS.gene056215	MS.gene48389	0.801442	8.99E-49	-1.69E-46
MS.gene056366	MS.gene48389	0.85004	2.20E-60	-1.69E-46
MS.gene056498	MS.gene48389	0.823456	1.37E-53	-1.69E-46
MS.gene056506	MS.gene48389	0.806978	6.32E-50	-1.69E-46
MS.gene056776	MS.gene48389	0.825327	4.98E-54	-1.69E-46
MS.gene05686	MS.gene48389	0.823619	1.26E-53	-1.69E-46
MS.gene05690	MS.gene48389	0.812697	3.72E-51	-1.69E-46
MS.gene057026	MS.gene48389	0.806936	6.46E-50	-1.69E-46
MS.gene057027	MS.gene48389	0.808473	3.04E-50	-1.69E-46
MS.gene057060	MS.gene48389	0.844731	6.30E-59	-1.69E-46
MS.gene057102	MS.gene48389	0.803199	3.91E-49	-1.69E-46
MS.gene057208	MS.gene48389	0.849507	3.09E-60	-1.69E-46
MS.gene057314	MS.gene48389	0.806776	6.98E-50	-1.69E-46
MS.gene057414	MS.gene48389	0.809547	1.79E-50	-1.69E-46
MS.gene057444	MS.gene48389	0.841203	5.48E-58	-1.69E-46
MS.gene057555	MS.gene48389	0.802672	5.02E-49	-1.69E-46
MS.gene057611	MS.gene48389	0.805989	1.02E-49	-1.69E-46
MS.gene057693	MS.gene48389	0.825319	5.00E-54	-1.69E-46
MS.gene057705	MS.gene48389	0.807531	4.83E-50	-1.69E-46
MS.gene058008	MS.gene48389	0.800965	1.13E-48	-1.69E-46
MS.gene058037	MS.gene48389	0.842126	3.13E-58	-1.69E-46
MS.gene058279	MS.gene48389	0.807703	4.44E-50	-1.69E-46
MS.gene058294	MS.gene48389	0.805427	1.34E-49	-1.69E-46
MS.gene058625	MS.gene48389	0.848277	6.79E-60	-1.69E-46
MS.gene058783	MS.gene48389	0.80214	6.46E-49	-1.69E-46
MS.gene058979	MS.gene48389	0.816347	5.79E-52	-1.69E-46
MS.gene059272	MS.gene48389	0.805485	1.30E-49	-1.69E-46
MS.gene05950	MS.gene48389	0.813956	1.97E-51	-1.69E-46
MS.gene05953	MS.gene48389	0.823512	1.33E-53	-1.69E-46
MS.gene05954	MS.gene48389	0.860797	1.61E-63	-1.69E-46
MS.gene059584	MS.gene48389	0.822496	2.30E-53	-1.69E-46
MS.gene059625	MS.gene48389	0.831235	1.87E-55	-1.69E-46
MS.gene059935	MS.gene48389	0.813754	2.18E-51	-1.69E-46
MS.gene060322	MS.gene48389	0.816815	4.55E-52	-1.69E-46
MS.gene060365	MS.gene48389	0.826443	2.71E-54	-1.69E-46
MS.gene060429	MS.gene48389	0.84034	9.22E-58	-1.69E-46
MS.gene060521	MS.gene48389	0.858376	8.60E-63	-1.69E-46
MS.gene060572	MS.gene48389	0.817622	3.00E-52	-1.69E-46
MS.gene060634	MS.gene48389	-0.837115	6.29E-57	-1.69E-46
MS.gene060879	MS.gene48389	0.805043	1.61E-49	-1.69E-46
MS.gene061040	MS.gene48389	-0.824407	8.21E-54	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene48389	MS.gene051630	PPI
MS.gene06729	MS.gene48389	PPI
MS.gene48389	MS.gene44230	PPI
MS.gene48389	MS.gene48281	PPI
MS.gene48389	MS.gene057144	PPI
MS.gene48389	MS.gene064786	PPI
MS.gene58134	MS.gene48389	PPI
MS.gene48389	MS.gene52815	PPI
MS.gene48389	MS.gene033335	PPI
MS.gene48389	MS.gene68079	PPI
MS.gene48389	MS.gene89672	PPI
MS.gene48389	MS.gene69373	PPI
MS.gene48389	MS.gene70283	PPI
MS.gene48389	MS.gene70282	PPI
MS.gene48389	MS.gene030685	PPI
MS.gene48389	MS.gene064694	PPI
MS.gene48389	MS.gene004328	PPI
MS.gene40486	MS.gene48389	PPI
MS.gene06946	MS.gene48389	PPI
MS.gene48389	MS.gene69461	PPI
MS.gene48389	MS.gene062770	PPI
MS.gene48389	MS.gene013607	PPI
MS.gene48389	MS.gene24375	PPI
MS.gene48389	MS.gene014485	PPI
MS.gene011376	MS.gene48389	PPI
MS.gene48389	MS.gene01284	PPI
MS.gene28454	MS.gene48389	PPI
MS.gene48389	MS.gene64789	PPI
MS.gene48389	MS.gene57218	PPI
MS.gene32974	MS.gene48389	PPI
MS.gene48389	MS.gene36633	PPI
MS.gene48389	MS.gene024828	PPI
MS.gene48389	MS.gene28183	PPI
MS.gene48389	MS.gene01697	PPI
MS.gene48389	MS.gene057145	PPI
MS.gene48389	MS.gene02265	PPI
MS.gene67460	MS.gene48389	PPI
MS.gene48389	MS.gene21169	PPI
MS.gene48389	MS.gene017622	PPI
MS.gene48389	MS.gene025808	PPI
MS.gene48389	MS.gene002306	PPI
MS.gene48389	MS.gene058061	PPI
MS.gene48389	MS.gene072220	PPI
MS.gene48389	MS.gene012127	PPI
MS.gene48389	MS.gene072221	PPI
MS.gene48389	MS.gene011065	PPI
MS.gene48389	MS.gene21801	PPI
MS.gene48389	MS.gene48279	PPI
MS.gene48389	MS.gene014393	PPI
MS.gene48389	MS.gene044013	PPI
MS.gene48389	MS.gene58136	PPI
MS.gene48389	MS.gene09974	PPI
MS.gene48400	MS.gene48389	PPI
MS.gene48389	MS.gene67099	PPI
MS.gene48389	MS.gene24542	PPI
MS.gene48389	MS.gene36746	PPI
MS.gene48389	MS.gene42312	PPI
MS.gene48389	MS.gene59485	PPI
MS.gene48389	MS.gene030686	PPI
MS.gene48389	MS.gene002648	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 17 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGACGTTCTCGGAAGGTTT+TGG	0.276388	8.3:-17068118	None:intergenic
CTCAGCAATGGGAAGATGAT+TGG	0.389575	8.3:+17068373	MS.gene48389:CDS
TCAGCTAAAGAGAGAGTTAG+AGG	0.407568	8.3:+17068428	MS.gene48389:CDS
GAGATCAACCTTGACGTTCT+CGG	0.418361	8.3:-17068128	None:intergenic
AGACAAGGAGGAAGGGAGAG+AGG	0.431303	8.3:+17068347	MS.gene48389:CDS
TGAAGACGACGACGAGTTCG+AGG	0.463513	8.3:+17068153	MS.gene48389:CDS
TCAGCAATGGGAAGATGATT+GGG	0.477084	8.3:+17068374	MS.gene48389:CDS
GGAGAGAGGTAGCTCAGCAA+TGG	0.520450	8.3:+17068361	MS.gene48389:CDS
GGTTGGGAAGACAAGGAGGA+AGG	0.522923	8.3:+17068339	MS.gene48389:intron
GAGAGAGGTAGCTCAGCAAT+GGG	0.525728	8.3:+17068362	MS.gene48389:CDS
GTTGGGAAGACAAGGAGGAA+GGG	0.529450	8.3:+17068340	MS.gene48389:CDS
TCAACCTTGACGTTCTCGGA+AGG	0.583809	8.3:-17068124	None:intergenic
ACTTGCAGGTTGGGAAGACA+AGG	0.596138	8.3:+17068332	MS.gene48389:intron
CAGCTAAAGAGAGAGTTAGA+GGG	0.638489	8.3:+17068429	MS.gene48389:CDS
GAGGAGTTTGAAATCAACGA+AGG	0.657612	8.3:+17068172	MS.gene48389:CDS
TGCAGGTTGGGAAGACAAGG+AGG	0.659645	8.3:+17068335	MS.gene48389:intron
AAAACCTTCCGAGAACGTCA+AGG	0.660047	8.3:+17068120	MS.gene48389:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TACTAACATGAATTCAAATC+TGG	-	chr8.3:17068279-17068298	None:intergenic	25.0%
	AAATTGATTGCAACAACGAT+TGG	-	chr8.3:17068236-17068255	None:intergenic	30.0%
	AACAACGATTGGAATGAAGA+GGG	-	chr8.3:17068225-17068244	None:intergenic	35.0%
!	ATCTTATGTAACTTGCAGGT+TGG	+	chr8.3:17068322-17068341	MS.gene48389:intron	35.0%
!	TCTTATGTAACTTGCAGGTT+GGG	+	chr8.3:17068323-17068342	MS.gene48389:intron	35.0%
!	TGCAATCTTATGTAACTTGC+AGG	+	chr8.3:17068318-17068337	MS.gene48389:intron	35.0%
	AATGAAGAGGGGTTAAAGGT+TGG	-	chr8.3:17068213-17068232	None:intergenic	40.0%
	ACAACGATTGGAATGAAGAG+GGG	-	chr8.3:17068224-17068243	None:intergenic	40.0%
	CAACAACGATTGGAATGAAG+AGG	-	chr8.3:17068226-17068245	None:intergenic	40.0%
	CAGCTAAAGAGAGAGTTAGA+GGG	+	chr8.3:17068429-17068448	MS.gene48389:CDS	40.0%
	TCAGCAATGGGAAGATGATT+GGG	+	chr8.3:17068374-17068393	MS.gene48389:CDS	40.0%
	TCAGCTAAAGAGAGAGTTAG+AGG	+	chr8.3:17068428-17068447	MS.gene48389:CDS	40.0%
	TTGGAATGAAGAGGGGTTAA+AGG	-	chr8.3:17068217-17068236	None:intergenic	40.0%
!	GAGGAGTTTGAAATCAACGA+AGG	+	chr8.3:17068172-17068191	MS.gene48389:CDS	40.0%
	AAAACCTTCCGAGAACGTCA+AGG	+	chr8.3:17068120-17068139	MS.gene48389:CDS	45.0%
	CTCAGCAATGGGAAGATGAT+TGG	+	chr8.3:17068373-17068392	MS.gene48389:CDS	45.0%
	GAGATCAACCTTGACGTTCT+CGG	-	chr8.3:17068131-17068150	None:intergenic	45.0%
!	TTGACGTTCTCGGAAGGTTT+TGG	-	chr8.3:17068121-17068140	None:intergenic	45.0%
	GAGAGAGGTAGCTCAGCAAT+GGG	+	chr8.3:17068362-17068381	MS.gene48389:CDS	50.0%
	GTTGGGAAGACAAGGAGGAA+GGG	+	chr8.3:17068340-17068359	MS.gene48389:CDS	50.0%
	TCAACCTTGACGTTCTCGGA+AGG	-	chr8.3:17068127-17068146	None:intergenic	50.0%
!	ACTTGCAGGTTGGGAAGACA+AGG	+	chr8.3:17068332-17068351	MS.gene48389:intron	50.0%
!!!	GTTCTCGGAAGGTTTTGGCT+CGG	-	chr8.3:17068116-17068135	None:intergenic	50.0%
	AGACAAGGAGGAAGGGAGAG+AGG	+	chr8.3:17068347-17068366	MS.gene48389:CDS	55.0%
	GGAGAGAGGTAGCTCAGCAA+TGG	+	chr8.3:17068361-17068380	MS.gene48389:CDS	55.0%
	GGTTGGGAAGACAAGGAGGA+AGG	+	chr8.3:17068339-17068358	MS.gene48389:intron	55.0%
	TGAAGACGACGACGAGTTCG+AGG	+	chr8.3:17068153-17068172	MS.gene48389:CDS	55.0%
!	TGCAGGTTGGGAAGACAAGG+AGG	+	chr8.3:17068335-17068354	MS.gene48389:intron	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.3	gene	17068106	17068470	17068106	ID=MS.gene48389
chr8.3	mRNA	17068106	17068470	17068106	ID=MS.gene48389.t1;Parent=MS.gene48389
chr8.3	exon	17068106	17068193	17068106	ID=MS.gene48389.t1.exon1;Parent=MS.gene48389.t1
chr8.3	CDS	17068106	17068193	17068106	ID=cds.MS.gene48389.t1;Parent=MS.gene48389.t1
chr8.3	exon	17068340	17068470	17068340	ID=MS.gene48389.t1.exon2;Parent=MS.gene48389.t1
chr8.3	CDS	17068340	17068470	17068340	ID=cds.MS.gene48389.t1;Parent=MS.gene48389.t1

Gene Sequence	>MS.gene48389 ATGGCAACCGAGCCAAAACCTTCCGAGAACGTCAAGGTTGATCTCTTTGAAGACGACGACGAGTTCGAGGAGTTTGAAATCAACGAAGGTAACTTCAAATCGTTCCAACCTTTAACCCCTCTTCATTCCAATCGTTGTTGCAATCAATTTCAGCTTCATTCAATTTCGATCCAGATTTGAATTCATGTTAGTAGTTTCTTATTGTTTATTTTTGCAATCTTATGTAACTTGCAGGTTGGGAAGACAAGGAGGAAGGGAGAGAGGTAGCTCAGCAATGGGAAGATGATTGGGATGATGATGATGTTAGTGATGATTTCTCTCTTCAGCTAAAGAGAGAGTTAGAGGGCAATGCTGAGAAGAAATAA
Protein sequence	>MS.gene48389.t1 MATEPKPSENVKVDLFEDDDEFEEFEINEGWEDKEEGREVAQQWEDDWDDDDVSDDFSLQLKRELEGNAEKK