AlfalfaGEDB | MS.gene074369

M. sativa cultivar XinJiangDaYe / MS.gene074369

KEGG: K10881
GO: GO:0005575 GO:0006405 GO:0006406 GO:0006810 GO:0006913 GO:0008150 GO:0008541 GO:0009987 GO:0015931 GO:0016043 GO:0022607 GO:0032991 GO:0034622 GO:0043248 GO:0043933 GO:0046907 GO:0050657 GO:0050658 GO:0051028 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051641 GO:0065003 GO:0071702 GO:0071705 GO:0071840
PFAM: PF05160.16

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene074369.t1	XP_013461011.1	100	73	0	0	1	73	1	73	9.20E-08	65.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene074369.t1	I3SYL1	100.0	73	0	0	1	73	1	73	6.6e-08	65.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049140	MS.gene074369	0.820133	8.06E-53	-1.69E-46
MS.gene049166	MS.gene074369	0.819958	8.83E-53	-1.69E-46
MS.gene049756	MS.gene074369	0.842599	2.34E-58	-1.69E-46
MS.gene049861	MS.gene074369	0.819577	1.08E-52	-1.69E-46
MS.gene050114	MS.gene074369	0.838801	2.32E-57	-1.69E-46
MS.gene050277	MS.gene074369	0.842158	3.07E-58	-1.69E-46
MS.gene050303	MS.gene074369	0.8464	2.22E-59	-1.69E-46
MS.gene050317	MS.gene074369	0.858144	1.01E-62	-1.69E-46
MS.gene050320	MS.gene074369	0.832379	9.78E-56	-1.69E-46
MS.gene050752	MS.gene074369	0.803413	3.53E-49	-1.69E-46
MS.gene051003	MS.gene074369	0.809933	1.48E-50	-1.69E-46
MS.gene051245	MS.gene074369	0.844708	6.40E-59	-1.69E-46
MS.gene051451	MS.gene074369	0.837666	4.54E-57	-1.69E-46
MS.gene051452	MS.gene074369	0.800153	1.65E-48	-1.69E-46
MS.gene051655	MS.gene074369	0.802207	6.26E-49	-1.69E-46
MS.gene051746	MS.gene074369	0.846213	2.50E-59	-1.69E-46
MS.gene051748	MS.gene074369	0.855112	7.85E-62	-1.69E-46
MS.gene051756	MS.gene074369	0.831862	1.31E-55	-1.69E-46
MS.gene051757	MS.gene074369	0.827366	1.63E-54	-1.69E-46
MS.gene05188	MS.gene074369	0.834071	3.71E-56	-1.69E-46
MS.gene051903	MS.gene074369	0.832345	9.97E-56	-1.69E-46
MS.gene051975	MS.gene074369	0.85388	1.78E-61	-1.69E-46
MS.gene052286	MS.gene074369	0.809278	2.05E-50	-1.69E-46
MS.gene052434	MS.gene074369	0.860474	2.01E-63	-1.69E-46
MS.gene052947	MS.gene074369	0.817701	2.88E-52	-1.69E-46
MS.gene053058	MS.gene074369	0.8203	7.38E-53	-1.69E-46
MS.gene053109	MS.gene074369	0.853695	2.02E-61	-1.69E-46
MS.gene053298	MS.gene074369	0.806063	9.87E-50	-1.69E-46
MS.gene053381	MS.gene074369	0.826056	3.34E-54	-1.69E-46
MS.gene053564	MS.gene074369	0.806906	6.55E-50	-1.69E-46
MS.gene054403	MS.gene074369	0.80206	6.71E-49	-1.69E-46
MS.gene05469	MS.gene074369	0.836079	1.15E-56	-1.69E-46
MS.gene05517	MS.gene074369	0.812105	5.01E-51	-1.69E-46
MS.gene055311	MS.gene074369	0.825881	3.68E-54	-1.69E-46
MS.gene055313	MS.gene074369	0.80039	1.47E-48	-1.69E-46
MS.gene055441	MS.gene074369	0.805306	1.42E-49	-1.69E-46
MS.gene055603	MS.gene074369	0.847435	1.16E-59	-1.69E-46
MS.gene056403	MS.gene074369	0.828005	1.14E-54	-1.69E-46
MS.gene056580	MS.gene074369	0.811442	6.99E-51	-1.69E-46
MS.gene056605	MS.gene074369	0.841569	4.39E-58	-1.69E-46
MS.gene056631	MS.gene074369	0.856613	2.86E-62	-1.69E-46
MS.gene056638	MS.gene074369	0.814828	1.26E-51	-1.69E-46
MS.gene056752	MS.gene074369	0.809234	2.09E-50	-1.69E-46
MS.gene056787	MS.gene074369	0.822898	1.85E-53	-1.69E-46
MS.gene056849	MS.gene074369	0.802363	5.81E-49	-1.69E-46
MS.gene056986	MS.gene074369	0.880096	7.27E-70	-1.69E-46
MS.gene057079	MS.gene074369	0.812566	3.98E-51	-1.69E-46
MS.gene057094	MS.gene074369	0.849465	3.18E-60	-1.69E-46
MS.gene057140	MS.gene074369	0.823587	1.28E-53	-1.69E-46
MS.gene057234	MS.gene074369	0.837145	6.18E-57	-1.69E-46
MS.gene057360	MS.gene074369	0.816006	6.91E-52	-1.69E-46
MS.gene05781	MS.gene074369	0.80791	4.01E-50	-1.69E-46
MS.gene057999	MS.gene074369	0.847986	8.17E-60	-1.69E-46
MS.gene058084	MS.gene074369	0.817262	3.61E-52	-1.69E-46
MS.gene058238	MS.gene074369	0.87557	2.78E-68	-1.69E-46
MS.gene058906	MS.gene074369	0.827873	1.23E-54	-1.69E-46
MS.gene059146	MS.gene074369	0.80635	8.59E-50	-1.69E-46
MS.gene059249	MS.gene074369	0.80924	2.09E-50	-1.69E-46
MS.gene059360	MS.gene074369	0.829931	3.91E-55	-1.69E-46
MS.gene059830	MS.gene074369	0.865526	5.54E-65	-1.69E-46
MS.gene06001	MS.gene074369	0.839764	1.30E-57	-1.69E-46
MS.gene06010	MS.gene074369	0.830715	2.51E-55	-1.69E-46
MS.gene06011	MS.gene074369	0.88362	3.85E-71	-1.69E-46
MS.gene060297	MS.gene074369	0.808063	3.72E-50	-1.69E-46
MS.gene060586	MS.gene074369	0.871219	8.07E-67	-1.69E-46
MS.gene060702	MS.gene074369	0.825199	5.34E-54	-1.69E-46
MS.gene060763	MS.gene074369	0.833838	4.24E-56	-1.69E-46
MS.gene060830	MS.gene074369	0.820238	7.62E-53	-1.69E-46
MS.gene060869	MS.gene074369	0.829079	6.29E-55	-1.69E-46
MS.gene060870	MS.gene074369	0.812986	3.22E-51	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene074369	MS.gene064786	PPI
MS.gene011376	MS.gene074369	PPI
MS.gene074369	MS.gene004328	PPI
MS.gene074369	MS.gene52815	PPI
MS.gene40486	MS.gene074369	PPI
MS.gene67460	MS.gene074369	PPI
MS.gene06946	MS.gene074369	PPI
MS.gene074369	MS.gene68079	PPI
MS.gene074369	MS.gene89672	PPI
MS.gene074369	MS.gene69373	PPI
MS.gene074369	MS.gene70283	PPI
MS.gene074369	MS.gene28183	PPI
MS.gene074369	MS.gene70282	PPI
MS.gene074369	MS.gene030685	PPI
MS.gene28454	MS.gene074369	PPI
MS.gene074369	MS.gene064694	PPI
MS.gene32974	MS.gene074369	PPI
MS.gene074369	MS.gene69461	PPI
MS.gene074369	MS.gene062770	PPI
MS.gene074369	MS.gene013607	PPI
MS.gene074369	MS.gene24375	PPI
MS.gene074369	MS.gene014485	PPI
MS.gene074369	MS.gene01284	PPI
MS.gene074369	MS.gene64789	PPI
MS.gene074369	MS.gene48279	PPI
MS.gene074369	MS.gene57218	PPI
MS.gene074369	MS.gene36633	PPI
MS.gene074369	MS.gene024828	PPI
MS.gene074369	MS.gene01697	PPI
MS.gene074369	MS.gene057145	PPI
MS.gene074369	MS.gene02265	PPI
MS.gene074369	MS.gene36746	PPI
MS.gene074369	MS.gene030686	PPI
MS.gene074369	MS.gene21169	PPI
MS.gene074369	MS.gene017622	PPI
MS.gene074369	MS.gene025808	PPI
MS.gene48400	MS.gene074369	PPI
MS.gene074369	MS.gene002306	PPI
MS.gene074369	MS.gene058061	PPI
MS.gene074369	MS.gene072220	PPI
MS.gene074369	MS.gene012127	PPI
MS.gene074369	MS.gene072221	PPI
MS.gene074369	MS.gene011065	PPI
MS.gene074369	MS.gene21801	PPI
MS.gene06729	MS.gene074369	PPI
MS.gene074369	MS.gene014393	PPI
MS.gene074369	MS.gene044013	PPI
MS.gene074369	MS.gene58136	PPI
MS.gene074369	MS.gene09974	PPI
MS.gene074369	MS.gene67099	PPI
MS.gene074369	MS.gene24542	PPI
MS.gene58134	MS.gene074369	PPI
MS.gene074369	MS.gene033335	PPI
MS.gene074369	MS.gene42312	PPI
MS.gene074369	MS.gene59485	PPI
MS.gene074369	MS.gene002648	PPI
MS.gene074369	MS.gene051630	PPI
MS.gene074369	MS.gene44230	PPI
MS.gene074369	MS.gene48281	PPI
MS.gene074369	MS.gene057144	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene074369.t1	MTR_3g078130	100.000	73	0	0	1	73	1	73	4.87e-44	136

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene074369.t1	AT1G64750	76.712	73	16	1	1	72	1	73	3.24e-11	53.5
MS.gene074369.t1	AT1G64750	76.712	73	16	1	1	72	1	73	3.24e-11	53.5

Find 17 sgRNAs with CRISPR-Local

Find 58 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCAACAATGGGAGGATGAT+TGG	0.329365	3.4:-74882854	MS.gene074369:CDS
TCAACAATGGGAGGATGATT+GGG	0.443446	3.4:-74882853	MS.gene074369:CDS
ATTTCTCTGTTCAGCTGAGA+AGG	0.461195	3.4:-74882809	MS.gene074369:CDS
TGTTCAGCTGAGAAGGGAGT+TGG	0.494913	3.4:-74882802	MS.gene074369:CDS
GAAAGAAGTAACTCAACAAT+GGG	0.498445	3.4:-74882865	MS.gene074369:CDS
GAAGAGTTTGAAATCAATGA+AGG	0.502774	3.4:-74883891	MS.gene074369:intron
GAGTGGGATGACATGGAGGA+AGG	0.532354	3.4:-74882888	MS.gene074369:CDS
TTTCTCTGTTCAGCTGAGAA+GGG	0.558420	3.4:-74882808	MS.gene074369:CDS
AGCCTTTGCTTCAGTCGCCA+TGG	0.560336	3.4:+74883962	None:intergenic
GGAAAGAAGTAACTCAACAA+TGG	0.565472	3.4:-74882866	MS.gene074369:CDS
ATCCATGGCGACTGAAGCAA+AGG	0.576258	3.4:-74883964	None:intergenic
ACTTGCAGAGTGGGATGACA+TGG	0.578383	3.4:-74882895	MS.gene074369:intron
AGTGGGATGACATGGAGGAA+GGG	0.591985	3.4:-74882887	MS.gene074369:CDS
TGAAGCAAAGGCTGTGACCG+AGG	0.600680	3.4:-74883952	MS.gene074369:CDS
TAGATCGATCTTGACATCCT+CGG	0.618819	3.4:+74883935	None:intergenic
AGAAGTAACTCAACAATGGG+AGG	0.631574	3.4:-74882862	MS.gene074369:CDS
TGCAGAGTGGGATGACATGG+AGG	0.657206	3.4:-74882892	MS.gene074369:intron

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAGTTTCAAATTACATGATT+GGG	+	chr3.4:74883330-74883349	None:intergenic	20.0%
!!	TAAGTTTCAAATTACATGAT+TGG	+	chr3.4:74883331-74883350	None:intergenic	20.0%
!	AACATCACTGAAAATTTCTT+AGG	+	chr3.4:74882951-74882970	None:intergenic	25.0%
!	ATTGGCATTTGTATGTTAAT+TGG	-	chr3.4:74883704-74883723	MS.gene074369:intron	25.0%
!	ATTTCATACACGAAATAAAG+TGG	+	chr3.4:74883371-74883390	None:intergenic	25.0%
!	CTTATACATAAGCACTTATT+TGG	-	chr3.4:74883251-74883270	MS.gene074369:intron	25.0%
!	GTTTGGCATTCAATTAATTA+CGG	-	chr3.4:74883074-74883093	MS.gene074369:intron	25.0%
!	TTTGGCATTCAATTAATTAC+GGG	-	chr3.4:74883075-74883094	MS.gene074369:intron	25.0%
!!	ATTATCTTTGCTTCTGTTTT+AGG	-	chr3.4:74882905-74882924	MS.gene074369:intron	25.0%
!!!	TGAATCTCATTTAGTTTGAT+TGG	-	chr3.4:74883686-74883705	MS.gene074369:intron	25.0%
	AAGCAAAGATAATTGCATCA+GGG	+	chr3.4:74882898-74882917	None:intergenic	30.0%
	ACAGCACTTATATACTAGAT+AGG	+	chr3.4:74883118-74883137	None:intergenic	30.0%
	ATTATGCATGAACACTTCTA+GGG	-	chr3.4:74883635-74883654	MS.gene074369:intron	30.0%
	CAAATTACATGATTGGGTTA+TGG	+	chr3.4:74883324-74883343	None:intergenic	30.0%
	GAAAGAAGTAACTCAACAAT+GGG	-	chr3.4:74883874-74883893	MS.gene074369:intron	30.0%
	GCTGCATCATTTATATAATC+AGG	+	chr3.4:74883473-74883492	None:intergenic	30.0%
	GTTCATGCATAATGAGAAAT+AGG	+	chr3.4:74883628-74883647	None:intergenic	30.0%
	TTTCTTGACGACTTGAAATT+AGG	-	chr3.4:74883746-74883765	MS.gene074369:intron	30.0%
!	GAACATTTGTTAGACTGTTT+GGG	-	chr3.4:74883155-74883174	MS.gene074369:intron	30.0%
!	GAAGAGTTTGAAATCAATGA+AGG	-	chr3.4:74882848-74882867	MS.gene074369:CDS	30.0%
!	TCTAGTATATAAGTGCTGTT+TGG	-	chr3.4:74883119-74883138	MS.gene074369:intron	30.0%
!	TGAACATTTGTTAGACTGTT+TGG	-	chr3.4:74883154-74883173	MS.gene074369:intron	30.0%
	ACATTAGATTGTGAGTGCAA+TGG	+	chr3.4:74883788-74883807	None:intergenic	35.0%
	ATACACGAAATAAAGTGGTG+AGG	+	chr3.4:74883366-74883385	None:intergenic	35.0%
	ATCCTAATTCGTTTCTTCCA+CGG	+	chr3.4:74883014-74883033	None:intergenic	35.0%
	CATTATGCATGAACACTTCT+AGG	-	chr3.4:74883634-74883653	MS.gene074369:intron	35.0%
	GAAGCAAAGATAATTGCATC+AGG	+	chr3.4:74882899-74882918	None:intergenic	35.0%
	GGAAAGAAGTAACTCAACAA+TGG	-	chr3.4:74883873-74883892	MS.gene074369:intron	35.0%
	TAATTGCATCAGGGAAGAAA+CGG	+	chr3.4:74882889-74882908	None:intergenic	35.0%
	TTACATGATTGGGTTATGGT+TGG	+	chr3.4:74883320-74883339	None:intergenic	35.0%
	TTCAAGTCGTCAAGAAAAGT+TGG	+	chr3.4:74883743-74883762	None:intergenic	35.0%
!	GACTTGAAATTAGGAGGTTT+TGG	-	chr3.4:74883755-74883774	MS.gene074369:intron	35.0%
!	TGTTTTACAACTTGCAGAGT+GGG	-	chr3.4:74883835-74883854	MS.gene074369:intron	35.0%
!!	ACTTCTAGGGCGAAAATTAT+AGG	-	chr3.4:74883648-74883667	MS.gene074369:intron	35.0%
!!	TATTCATTAGTTTGCTTCCG+TGG	-	chr3.4:74882994-74883013	MS.gene074369:intron	35.0%
!!	TTGTTTTACAACTTGCAGAG+TGG	-	chr3.4:74883834-74883853	MS.gene074369:intron	35.0%
	AATTGCATCAGGGAAGAAAC+GGG	+	chr3.4:74882888-74882907	None:intergenic	40.0%
	AGAAGTAACTCAACAATGGG+AGG	-	chr3.4:74883877-74883896	MS.gene074369:intron	40.0%
	AGACTGTTTGGGAGAACTTA+TGG	-	chr3.4:74883166-74883185	MS.gene074369:intron	40.0%
	ATTTCTCTGTTCAGCTGAGA+AGG	-	chr3.4:74883930-74883949	MS.gene074369:CDS	40.0%
	CTTGACGACTTGAAATTAGG+AGG	-	chr3.4:74883749-74883768	MS.gene074369:intron	40.0%
	GGCATTCAATTAATTACGGG+AGG	-	chr3.4:74883078-74883097	MS.gene074369:intron	40.0%
	GTCGTCAAGAAAAGTTGGAT+AGG	+	chr3.4:74883738-74883757	None:intergenic	40.0%
	TAGATCGATCTTGACATCCT+CGG	+	chr3.4:74882807-74882826	None:intergenic	40.0%
	TCAACAATGGGAGGATGATT+GGG	-	chr3.4:74883886-74883905	MS.gene074369:intron	40.0%
	TGAGCTAGACTTTGTGTGTT+TGG	-	chr3.4:74883057-74883076	MS.gene074369:intron	40.0%
	TTCCGTGGAAGAAACGAATT+AGG	-	chr3.4:74883009-74883028	MS.gene074369:intron	40.0%
	TTTCTCTGTTCAGCTGAGAA+GGG	-	chr3.4:74883931-74883950	MS.gene074369:CDS	40.0%
	ATGCCACTACTCTCTTCTCA+TGG	-	chr3.4:74883594-74883613	MS.gene074369:intron	45.0%
	CTCAACAATGGGAGGATGAT+TGG	-	chr3.4:74883885-74883904	MS.gene074369:intron	45.0%
	TGCCACTACTCTCTTCTCAT+GGG	-	chr3.4:74883595-74883614	MS.gene074369:intron	45.0%
	ACTTGCAGAGTGGGATGACA+TGG	-	chr3.4:74883844-74883863	MS.gene074369:intron	50.0%
	AGTGGGATGACATGGAGGAA+GGG	-	chr3.4:74883852-74883871	MS.gene074369:intron	50.0%
	CACCCATGAGAAGAGAGTAG+TGG	+	chr3.4:74883600-74883619	None:intergenic	50.0%
	TGTTCAGCTGAGAAGGGAGT+TGG	-	chr3.4:74883937-74883956	MS.gene074369:CDS	50.0%
	GAGTGGGATGACATGGAGGA+AGG	-	chr3.4:74883851-74883870	MS.gene074369:intron	55.0%
	TGAAGCAAAGGCTGTGACCG+AGG	-	chr3.4:74882787-74882806	MS.gene074369:CDS	55.0%
	TGCAGAGTGGGATGACATGG+AGG	-	chr3.4:74883847-74883866	MS.gene074369:intron	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.4	gene	74882779	74883982	74882779	ID=MS.gene074369
chr3.4	mRNA	74882779	74883982	74882779	ID=MS.gene074369.t1;Parent=MS.gene074369
chr3.4	exon	74883892	74883982	74883892	ID=MS.gene074369.t1.exon1;Parent=MS.gene074369.t1
chr3.4	CDS	74883892	74883982	74883892	ID=cds.MS.gene074369.t1;Parent=MS.gene074369.t1
chr3.4	exon	74882779	74882909	74882779	ID=MS.gene074369.t1.exon2;Parent=MS.gene074369.t1
chr3.4	CDS	74882779	74882909	74882779	ID=cds.MS.gene074369.t1;Parent=MS.gene074369.t1

Gene Sequence

>MS.gene074369

ATGGCGACTGAAGCAAAGGCTGTGACCGAGGATGTCAAGATCGATCTATTCGAAGACGATGATGAGTTCGAAGAGTTTGAAATCAATGAAGGTATGAATCAGTAATCCCGTTTCTTCCCTGATGCAATTATCTTTGCTTCTGTTTTAGGTTTACATTTAGTTAAGTGCTCCTAAGAAATTTTCAGTGATGTTATCATCAAATGATTTATAAGTTATATTCATTAGTTTGCTTCCGTGGAAGAAACGAATTAGGATTTCATCGCGTATTGTTGCACATTTGAGCTAGACTTTGTGTGTTTGGCATTCAATTAATTACGGGAGGTTATGAAAATTTAACCTATCTAGTATATAAGTGCTGTTTGGATTTGAGTAACATGAACATTTGTTAGACTGTTTGGGAGAACTTATGGAAACAACTTATAACTTATATGAAAATAGTTTGAATTTATTTTATGCTTTGTTATAAAAATTGCTTATACATAAGCACTTATTTGGTAAGCGCTTGTGCTATAAGCACTTAATTAATTAAGCTGTATGTCCAACCATAACCCAATCATGTAATTTGAAACTTATTTGATACTTCACCTCACCACTTTATTTCGTGTATGAAATAATATGATATTTAAATATACATATCTACTATTAAATCATATATACTTGCAAATGTCTAAACTTTTATATACATTATTGTCCTGATTATATAAATGATGCAGCATTTAGATTTGCTAGATTCACGTTGTACACAGCGTTAATGAAGATGTAAGTTTGCTAATTTTTTGTGCGTGTGTCTCACAGTGCATGCAAACTGTAATTTTATGCCACTACTCTCTTCTCATGGGTGTTAATCCTATTTCTCATTATGCATGAACACTTCTAGGGCGAAAATTATAGGACTTATTTAATTGCTTGAATCTCATTTAGTTTGATTGGCATTTGTATGTTAATTGGCTTCACTGCCTATCCAACTTTTCTTGACGACTTGAAATTAGGAGGTTTTGGATTTCTGCCATTGCACTCACAATCTAATGTTGCTAACTATTTCTTTTTGCGCTTCTTTGTTTTACAACTTGCAGAGTGGGATGACATGGAGGAAGGGAAAGAAGTAACTCAACAATGGGAGGATGATTGGGATGATGATGATGTTAGCGATGATTTCTCTGTTCAGCTGAGAAGGGAGTTGGAGAAGACTACTGATAAGAATTAA

Protein sequence

>MS.gene074369.t1

MATEAKAVTEDVKIDLFEDDDEFEEFEINEEWDDMEEGKEVTQQWEDDWDDDDVSDDFSVQLRRELEKTTDKN