AlfalfaGEDB | MS.gene70613

M. sativa cultivar XinJiangDaYe / MS.gene70613

KEGG: K10881
GO: GO:0005575 GO:0006405 GO:0006406 GO:0006810 GO:0006913 GO:0008150 GO:0008541 GO:0009987 GO:0015931 GO:0016043 GO:0022607 GO:0032991 GO:0034622 GO:0043248 GO:0043933 GO:0046907 GO:0050657 GO:0050658 GO:0051028 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051641 GO:0065003 GO:0071702 GO:0071705 GO:0071840
PFAM: PF05160.16

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70613.t1	XP_013461011.1	100	73	0	0	1	73	1	73	9.20E-08	65.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70613.t1	I3SYL1	100.0	73	0	0	1	73	1	73	6.6e-08	65.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049140	MS.gene70613	0.82599	3.47E-54	-1.69E-46
MS.gene049166	MS.gene70613	0.814269	1.68E-51	-1.69E-46
MS.gene049756	MS.gene70613	0.84185	3.70E-58	-1.69E-46
MS.gene049861	MS.gene70613	0.818856	1.58E-52	-1.69E-46
MS.gene050114	MS.gene70613	0.834667	2.62E-56	-1.69E-46
MS.gene050277	MS.gene70613	0.849222	3.71E-60	-1.69E-46
MS.gene050303	MS.gene70613	0.852224	5.31E-61	-1.69E-46
MS.gene050317	MS.gene70613	0.850986	1.19E-60	-1.69E-46
MS.gene050320	MS.gene70613	0.830347	3.09E-55	-1.69E-46
MS.gene051003	MS.gene70613	0.805522	1.28E-49	-1.69E-46
MS.gene051245	MS.gene70613	0.844432	7.59E-59	-1.69E-46
MS.gene051451	MS.gene70613	0.835506	1.61E-56	-1.69E-46
MS.gene051452	MS.gene70613	0.805264	1.45E-49	-1.69E-46
MS.gene051633	MS.gene70613	0.80417	2.46E-49	-1.69E-46
MS.gene051676	MS.gene70613	0.800321	1.52E-48	-1.69E-46
MS.gene051746	MS.gene70613	0.84093	6.46E-58	-1.69E-46
MS.gene051748	MS.gene70613	0.851364	9.31E-61	-1.69E-46
MS.gene051756	MS.gene70613	0.829584	4.74E-55	-1.69E-46
MS.gene051757	MS.gene70613	0.827381	1.61E-54	-1.69E-46
MS.gene05188	MS.gene70613	0.831822	1.34E-55	-1.69E-46
MS.gene051903	MS.gene70613	0.833118	6.41E-56	-1.69E-46
MS.gene051975	MS.gene70613	0.852771	3.71E-61	-1.69E-46
MS.gene052286	MS.gene70613	0.809536	1.80E-50	-1.69E-46
MS.gene052376	MS.gene70613	0.80149	8.79E-49	-1.69E-46
MS.gene052434	MS.gene70613	0.858455	8.14E-63	-1.69E-46
MS.gene052947	MS.gene70613	0.813459	2.53E-51	-1.69E-46
MS.gene053058	MS.gene70613	0.822292	2.56E-53	-1.69E-46
MS.gene053109	MS.gene70613	0.852431	4.64E-61	-1.69E-46
MS.gene053298	MS.gene70613	0.806324	8.69E-50	-1.69E-46
MS.gene053381	MS.gene70613	0.819689	1.02E-52	-1.69E-46
MS.gene053564	MS.gene70613	0.803875	2.83E-49	-1.69E-46
MS.gene053952	MS.gene70613	0.801983	6.96E-49	-1.69E-46
MS.gene05469	MS.gene70613	0.830421	2.96E-55	-1.69E-46
MS.gene05517	MS.gene70613	0.816583	5.13E-52	-1.69E-46
MS.gene055311	MS.gene70613	0.820884	5.42E-53	-1.69E-46
MS.gene055441	MS.gene70613	0.808731	2.68E-50	-1.69E-46
MS.gene055603	MS.gene70613	0.851094	1.11E-60	-1.69E-46
MS.gene055780	MS.gene70613	0.800277	1.55E-48	-1.69E-46
MS.gene056403	MS.gene70613	0.832978	6.94E-56	-1.69E-46
MS.gene056580	MS.gene70613	0.807448	5.03E-50	-1.69E-46
MS.gene056605	MS.gene70613	0.8482	7.13E-60	-1.69E-46
MS.gene056631	MS.gene70613	0.857077	2.09E-62	-1.69E-46
MS.gene056638	MS.gene70613	0.808426	3.12E-50	-1.69E-46
MS.gene056752	MS.gene70613	0.801699	7.96E-49	-1.69E-46
MS.gene056787	MS.gene70613	0.820993	5.11E-53	-1.69E-46
MS.gene056849	MS.gene70613	0.801777	7.67E-49	-1.69E-46
MS.gene056986	MS.gene70613	0.879119	1.62E-69	-1.69E-46
MS.gene057057	MS.gene70613	0.800203	1.61E-48	-1.69E-46
MS.gene057079	MS.gene70613	0.820258	7.54E-53	-1.69E-46
MS.gene057094	MS.gene70613	0.853312	2.60E-61	-1.69E-46
MS.gene057140	MS.gene70613	0.824356	8.44E-54	-1.69E-46
MS.gene057234	MS.gene70613	0.836453	9.27E-57	-1.69E-46
MS.gene057360	MS.gene70613	0.809914	1.50E-50	-1.69E-46
MS.gene05781	MS.gene70613	0.803445	3.47E-49	-1.69E-46
MS.gene057999	MS.gene70613	0.851398	9.11E-61	-1.69E-46
MS.gene058084	MS.gene70613	0.822554	2.23E-53	-1.69E-46
MS.gene058238	MS.gene70613	0.879142	1.59E-69	-1.69E-46
MS.gene058906	MS.gene70613	0.830439	2.93E-55	-1.69E-46
MS.gene059146	MS.gene70613	0.803822	2.90E-49	-1.69E-46
MS.gene059249	MS.gene70613	0.816215	6.20E-52	-1.69E-46
MS.gene059360	MS.gene70613	0.823287	1.50E-53	-1.69E-46
MS.gene059830	MS.gene70613	0.874181	8.26E-68	-1.69E-46
MS.gene06001	MS.gene70613	0.83996	1.16E-57	-1.69E-46
MS.gene06010	MS.gene70613	0.825046	5.80E-54	-1.69E-46
MS.gene06011	MS.gene70613	0.87947	1.21E-69	-1.69E-46
MS.gene060297	MS.gene70613	0.815375	9.55E-52	-1.69E-46
MS.gene060586	MS.gene70613	0.872113	4.08E-67	-1.69E-46
MS.gene060702	MS.gene70613	0.819955	8.85E-53	-1.69E-46
MS.gene060763	MS.gene70613	0.830128	3.50E-55	-1.69E-46
MS.gene060830	MS.gene70613	0.830003	3.75E-55	-1.69E-46
MS.gene060869	MS.gene70613	0.822095	2.84E-53	-1.69E-46
MS.gene060870	MS.gene70613	0.805334	1.40E-49	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene70613	MS.gene058061	PPI
MS.gene70613	MS.gene072220	PPI
MS.gene70613	MS.gene012127	PPI
MS.gene70613	MS.gene057144	PPI
MS.gene70613	MS.gene064786	PPI
MS.gene70613	MS.gene072221	PPI
MS.gene70613	MS.gene011065	PPI
MS.gene06729	MS.gene70613	PPI
MS.gene70613	MS.gene67099	PPI
MS.gene70613	MS.gene033335	PPI
MS.gene70613	MS.gene42312	PPI
MS.gene70613	MS.gene59485	PPI
MS.gene70613	MS.gene051630	PPI
MS.gene58134	MS.gene70613	PPI
MS.gene40486	MS.gene70613	PPI
MS.gene70613	MS.gene48281	PPI
MS.gene06946	MS.gene70613	PPI
MS.gene70613	MS.gene70283	PPI
MS.gene70613	MS.gene70282	PPI
MS.gene70613	MS.gene064694	PPI
MS.gene70613	MS.gene004328	PPI
MS.gene011376	MS.gene70613	PPI
MS.gene70613	MS.gene69461	PPI
MS.gene70613	MS.gene52815	PPI
MS.gene32974	MS.gene70613	PPI
MS.gene70613	MS.gene013607	PPI
MS.gene70613	MS.gene68079	PPI
MS.gene70613	MS.gene014485	PPI
MS.gene70613	MS.gene01284	PPI
MS.gene67460	MS.gene70613	PPI
MS.gene70613	MS.gene89672	PPI
MS.gene70613	MS.gene69373	PPI
MS.gene70613	MS.gene57218	PPI
MS.gene70613	MS.gene024828	PPI
MS.gene70613	MS.gene28183	PPI
MS.gene70613	MS.gene030685	PPI
MS.gene70613	MS.gene02265	PPI
MS.gene70613	MS.gene21169	PPI
MS.gene70613	MS.gene062770	PPI
MS.gene70613	MS.gene017622	PPI
MS.gene70613	MS.gene025808	PPI
MS.gene70613	MS.gene24375	PPI
MS.gene48400	MS.gene70613	PPI
MS.gene70613	MS.gene21801	PPI
MS.gene70613	MS.gene64789	PPI
MS.gene70613	MS.gene48279	PPI
MS.gene70613	MS.gene36633	PPI
MS.gene70613	MS.gene014393	PPI
MS.gene70613	MS.gene044013	PPI
MS.gene70613	MS.gene58136	PPI
MS.gene70613	MS.gene09974	PPI
MS.gene70613	MS.gene01697	PPI
MS.gene28454	MS.gene70613	PPI
MS.gene70613	MS.gene057145	PPI
MS.gene70613	MS.gene24542	PPI
MS.gene70613	MS.gene36746	PPI
MS.gene70613	MS.gene030686	PPI
MS.gene70613	MS.gene002648	PPI
MS.gene70613	MS.gene44230	PPI
MS.gene70613	MS.gene002306	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70613.t1	MTR_3g078130	100.000	73	0	0	1	73	1	73	4.87e-44	136

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70613.t1	AT1G64750	76.712	73	16	1	1	72	1	73	3.24e-11	53.5
MS.gene70613.t1	AT1G64750	76.712	73	16	1	1	72	1	73	3.24e-11	53.5

Find 17 sgRNAs with CRISPR-Local

Find 58 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCAACAATGGGAGGATGAT+TGG	0.329365	3.1:-64136016	MS.gene70613:CDS
TCAACAATGGGAGGATGATT+GGG	0.443446	3.1:-64136015	MS.gene70613:CDS
ATTTCTCTGTTCAGCTGAGA+AGG	0.461195	3.1:-64135971	MS.gene70613:CDS
TGTTCAGCTGAGAAGGGAGT+TGG	0.494913	3.1:-64135964	MS.gene70613:CDS
GAAAGAAGTAACTCAACAAT+GGG	0.498445	3.1:-64136027	MS.gene70613:CDS
GAAGAGTTTGAAATCAATGA+AGG	0.502774	3.1:-64137053	MS.gene70613:intron
GAGTGGGATGACATGGAGGA+AGG	0.532354	3.1:-64136050	MS.gene70613:CDS
TTTCTCTGTTCAGCTGAGAA+GGG	0.558420	3.1:-64135970	MS.gene70613:CDS
AGCCTTTGCTTCAGTCGCCA+TGG	0.560336	3.1:+64137124	None:intergenic
GGAAAGAAGTAACTCAACAA+TGG	0.565472	3.1:-64136028	MS.gene70613:CDS
ATCCATGGCGACTGAAGCAA+AGG	0.576258	3.1:-64137126	None:intergenic
ACTTGCAGAGTGGGATGACA+TGG	0.578383	3.1:-64136057	MS.gene70613:intron
AGTGGGATGACATGGAGGAA+GGG	0.591985	3.1:-64136049	MS.gene70613:CDS
TGAAGCAAAGGCTGTGACCG+AGG	0.600680	3.1:-64137114	MS.gene70613:CDS
TAGATCGATCTTGACATCCT+CGG	0.618819	3.1:+64137097	None:intergenic
AGAAGTAACTCAACAATGGG+AGG	0.631574	3.1:-64136024	MS.gene70613:CDS
TGCAGAGTGGGATGACATGG+AGG	0.657206	3.1:-64136054	MS.gene70613:intron

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAGTTTCAAATTACATGATT+GGG	+	chr3.1:64136492-64136511	None:intergenic	20.0%
!!	TAAGTTTCAAATTACATGAT+TGG	+	chr3.1:64136493-64136512	None:intergenic	20.0%
!	AACATCACTGAAAATTTCTT+AGG	+	chr3.1:64136113-64136132	None:intergenic	25.0%
!	ATTGGCATTTGTATGTTAAT+TGG	-	chr3.1:64136866-64136885	MS.gene70613:intron	25.0%
!	ATTTCATACACGAAATAAAG+TGG	+	chr3.1:64136533-64136552	None:intergenic	25.0%
!	CTTATACATAAGCACTTATT+TGG	-	chr3.1:64136413-64136432	MS.gene70613:intron	25.0%
!	GTTTGGCATTCAATTAATTA+CGG	-	chr3.1:64136236-64136255	MS.gene70613:intron	25.0%
!	TTTGGCATTCAATTAATTAC+GGG	-	chr3.1:64136237-64136256	MS.gene70613:intron	25.0%
!!	ATTATCTTTGCTTCTGTTTT+AGG	-	chr3.1:64136067-64136086	MS.gene70613:intron	25.0%
!!!	TGAATCTCATTTAGTTTGAT+TGG	-	chr3.1:64136848-64136867	MS.gene70613:intron	25.0%
	AAGCAAAGATAATTGCATCA+GGG	+	chr3.1:64136060-64136079	None:intergenic	30.0%
	ACAGCACTTATATACTAGAT+AGG	+	chr3.1:64136280-64136299	None:intergenic	30.0%
	ATTATGCATGAACACTTCTA+GGG	-	chr3.1:64136797-64136816	MS.gene70613:intron	30.0%
	CAAATTACATGATTGGGTTA+TGG	+	chr3.1:64136486-64136505	None:intergenic	30.0%
	GAAAGAAGTAACTCAACAAT+GGG	-	chr3.1:64137036-64137055	MS.gene70613:intron	30.0%
	GCTGCATCATTTATATAATC+AGG	+	chr3.1:64136635-64136654	None:intergenic	30.0%
	GTTCATGCATAATGAGAAAT+AGG	+	chr3.1:64136790-64136809	None:intergenic	30.0%
	TTTCTTGACGACTTGAAATT+AGG	-	chr3.1:64136908-64136927	MS.gene70613:intron	30.0%
!	GAACATTTGTTAGACTGTTT+GGG	-	chr3.1:64136317-64136336	MS.gene70613:intron	30.0%
!	GAAGAGTTTGAAATCAATGA+AGG	-	chr3.1:64136010-64136029	MS.gene70613:CDS	30.0%
!	TCTAGTATATAAGTGCTGTT+TGG	-	chr3.1:64136281-64136300	MS.gene70613:intron	30.0%
!	TGAACATTTGTTAGACTGTT+TGG	-	chr3.1:64136316-64136335	MS.gene70613:intron	30.0%
	ACATTAGATTGTGAGTGCAA+TGG	+	chr3.1:64136950-64136969	None:intergenic	35.0%
	ATACACGAAATAAAGTGGTG+AGG	+	chr3.1:64136528-64136547	None:intergenic	35.0%
	ATCCTAATTCGTTTCTTCCA+CGG	+	chr3.1:64136176-64136195	None:intergenic	35.0%
	CATTATGCATGAACACTTCT+AGG	-	chr3.1:64136796-64136815	MS.gene70613:intron	35.0%
	GAAGCAAAGATAATTGCATC+AGG	+	chr3.1:64136061-64136080	None:intergenic	35.0%
	GGAAAGAAGTAACTCAACAA+TGG	-	chr3.1:64137035-64137054	MS.gene70613:intron	35.0%
	TAATTGCATCAGGGAAGAAA+CGG	+	chr3.1:64136051-64136070	None:intergenic	35.0%
	TTACATGATTGGGTTATGGT+TGG	+	chr3.1:64136482-64136501	None:intergenic	35.0%
	TTCAAGTCGTCAAGAAAAGT+TGG	+	chr3.1:64136905-64136924	None:intergenic	35.0%
!	GACTTGAAATTAGGAGGTTT+TGG	-	chr3.1:64136917-64136936	MS.gene70613:intron	35.0%
!	TGTTTTACAACTTGCAGAGT+GGG	-	chr3.1:64136997-64137016	MS.gene70613:intron	35.0%
!!	ACTTCTAGGGCGAAAATTAT+AGG	-	chr3.1:64136810-64136829	MS.gene70613:intron	35.0%
!!	TATTCATTAGTTTGCTTCCG+TGG	-	chr3.1:64136156-64136175	MS.gene70613:intron	35.0%
!!	TTGTTTTACAACTTGCAGAG+TGG	-	chr3.1:64136996-64137015	MS.gene70613:intron	35.0%
	AATTGCATCAGGGAAGAAAC+GGG	+	chr3.1:64136050-64136069	None:intergenic	40.0%
	AGAAGTAACTCAACAATGGG+AGG	-	chr3.1:64137039-64137058	MS.gene70613:intron	40.0%
	AGACTGTTTGGGAGAACTTA+TGG	-	chr3.1:64136328-64136347	MS.gene70613:intron	40.0%
	ATTTCTCTGTTCAGCTGAGA+AGG	-	chr3.1:64137092-64137111	MS.gene70613:CDS	40.0%
	CTTGACGACTTGAAATTAGG+AGG	-	chr3.1:64136911-64136930	MS.gene70613:intron	40.0%
	GGCATTCAATTAATTACGGG+AGG	-	chr3.1:64136240-64136259	MS.gene70613:intron	40.0%
	GTCGTCAAGAAAAGTTGGAT+AGG	+	chr3.1:64136900-64136919	None:intergenic	40.0%
	TAGATCGATCTTGACATCCT+CGG	+	chr3.1:64135969-64135988	None:intergenic	40.0%
	TCAACAATGGGAGGATGATT+GGG	-	chr3.1:64137048-64137067	MS.gene70613:intron	40.0%
	TGAGCTAGACTTTGTGTGTT+TGG	-	chr3.1:64136219-64136238	MS.gene70613:intron	40.0%
	TTCCGTGGAAGAAACGAATT+AGG	-	chr3.1:64136171-64136190	MS.gene70613:intron	40.0%
	TTTCTCTGTTCAGCTGAGAA+GGG	-	chr3.1:64137093-64137112	MS.gene70613:CDS	40.0%
	ATGCCACTACTCTCTTCTCA+TGG	-	chr3.1:64136756-64136775	MS.gene70613:intron	45.0%
	CTCAACAATGGGAGGATGAT+TGG	-	chr3.1:64137047-64137066	MS.gene70613:intron	45.0%
	TGCCACTACTCTCTTCTCAT+GGG	-	chr3.1:64136757-64136776	MS.gene70613:intron	45.0%
	ACTTGCAGAGTGGGATGACA+TGG	-	chr3.1:64137006-64137025	MS.gene70613:intron	50.0%
	AGTGGGATGACATGGAGGAA+GGG	-	chr3.1:64137014-64137033	MS.gene70613:intron	50.0%
	CACCCATGAGAAGAGAGTAG+TGG	+	chr3.1:64136762-64136781	None:intergenic	50.0%
	TGTTCAGCTGAGAAGGGAGT+TGG	-	chr3.1:64137099-64137118	MS.gene70613:CDS	50.0%
	GAGTGGGATGACATGGAGGA+AGG	-	chr3.1:64137013-64137032	MS.gene70613:intron	55.0%
	TGAAGCAAAGGCTGTGACCG+AGG	-	chr3.1:64135949-64135968	MS.gene70613:CDS	55.0%
	TGCAGAGTGGGATGACATGG+AGG	-	chr3.1:64137009-64137028	MS.gene70613:intron	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	64135941	64137144	64135941	ID=MS.gene70613
chr3.1	mRNA	64135941	64137144	64135941	ID=MS.gene70613.t1;Parent=MS.gene70613
chr3.1	exon	64137054	64137144	64137054	ID=MS.gene70613.t1.exon1;Parent=MS.gene70613.t1
chr3.1	CDS	64137054	64137144	64137054	ID=cds.MS.gene70613.t1;Parent=MS.gene70613.t1
chr3.1	exon	64135941	64136071	64135941	ID=MS.gene70613.t1.exon2;Parent=MS.gene70613.t1
chr3.1	CDS	64135941	64136071	64135941	ID=cds.MS.gene70613.t1;Parent=MS.gene70613.t1

Gene Sequence

>MS.gene70613

ATGGCGACTGAAGCAAAGGCTGTGACCGAGGATGTCAAGATCGATCTATTCGAAGACGATGATGAGTTCGAAGAGTTTGAAATCAATGAAGGTATGAATCAGTAATCCCGTTTCTTCCCTGATGCAATTATCTTTGCTTCTGTTTTAGGTTTACATTTAGTTAAGTGCTCCTAAGAAATTTTCAGTGATGTTATCATCAAATGATTTATAAGTTATATTCATTAGTTTGCTTCCGTGGAAGAAACGAATTAGGATTTCATCGCGTATTGTTGCACATTTGAGCTAGACTTTGTGTGTTTGGCATTCAATTAATTACGGGAGGTTATGAAAATTTAACCTATCTAGTATATAAGTGCTGTTTGGATTTGAGTAACATGAACATTTGTTAGACTGTTTGGGAGAACTTATGGAAACAACTTATAACTTATATGAAAATAGTTTGAATTTATTTTATGCTTTGTTATAAAAATTGCTTATACATAAGCACTTATTTGGTAAGCGCTTGTGCTATAAGCACTTAATTAATTAAGCTGTATGTCCAACCATAACCCAATCATGTAATTTGAAACTTATTTGATACTTCACCTCACCACTTTATTTCGTGTATGAAATAATATGATATTTAAATATACATATCTACTATTAAATCATATATACTTGCAAATGTCTAAACTTTTATATACATTATTGTCCTGATTATATAAATGATGCAGCATTTAGATTTGCTAGATTCACGTTGTACACAGCGTTAATGAAGATGTAAGTTTGCTAATTTTTTGTGCGTGTGTCTCACAGTGCATGCAAACTGTAATTTTATGCCACTACTCTCTTCTCATGGGTGTTAATCCTATTTCTCATTATGCATGAACACTTCTAGGGCGAAAATTATAGGACTTATTTAATTGCTTGAATCTCATTTAGTTTGATTGGCATTTGTATGTTAATTGGCTTCACTGCCTATCCAACTTTTCTTGACGACTTGAAATTAGGAGGTTTTGGATTTCTGCCATTGCACTCACAATCTAATGTTGCTAACTATTTCTTTTTGCGCTTCTTTGTTTTACAACTTGCAGAGTGGGATGACATGGAGGAAGGGAAAGAAGTAACTCAACAATGGGAGGATGATTGGGATGATGATGATGTTAGCGATGATTTCTCTGTTCAGCTGAGAAGGGAGTTGGAGAAGACTACTGATAAGAATTAA

Protein sequence

>MS.gene70613.t1

MATEAKAVTEDVKIDLFEDDDEFEEFEINEEWDDMEEGKEVTQQWEDDWDDDDVSDDFSVQLRRELEKTTDKN