AlfalfaGEDB | MS.gene56924

M. sativa cultivar XinJiangDaYe / MS.gene56924

KEGG: K10881
GO: GO:0005575 GO:0006405 GO:0006406 GO:0006810 GO:0006913 GO:0008150 GO:0008541 GO:0009987 GO:0015931 GO:0016043 GO:0022607 GO:0032991 GO:0034622 GO:0043248 GO:0043933 GO:0046907 GO:0050657 GO:0050658 GO:0051028 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051641 GO:0065003 GO:0071702 GO:0071705 GO:0071840
PFAM: PF05160.16

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene56924.t1	GAU19652.1	91.2	34	3	0	1	34	1	34	3.40E-07	63.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene56924.t1	Q9FL96	77.8	36	7	1	1	35	1	36	5.5e-06	51.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene56924.t1	A0A2Z6LNG4	91.2	34	3	0	1	34	1	34	2.5e-07	63.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04918	MS.gene56924	0.800386	1.48E-48	-1.69E-46
MS.gene049254	MS.gene56924	0.847033	1.49E-59	-1.69E-46
MS.gene049293	MS.gene56924	-0.808792	2.60E-50	-1.69E-46
MS.gene049404	MS.gene56924	0.823278	1.51E-53	-1.69E-46
MS.gene049499	MS.gene56924	0.816888	4.38E-52	-1.69E-46
MS.gene049711	MS.gene56924	0.803828	2.89E-49	-1.69E-46
MS.gene049825	MS.gene56924	0.835815	1.35E-56	-1.69E-46
MS.gene049873	MS.gene56924	0.820971	5.17E-53	-1.69E-46
MS.gene050026	MS.gene56924	0.806095	9.72E-50	-1.69E-46
MS.gene050559	MS.gene56924	0.836779	7.66E-57	-1.69E-46
MS.gene050561	MS.gene56924	0.826205	3.08E-54	-1.69E-46
MS.gene050562	MS.gene56924	0.81808	2.36E-52	-1.69E-46
MS.gene051247	MS.gene56924	0.810948	8.94E-51	-1.69E-46
MS.gene051279	MS.gene56924	0.825325	4.99E-54	-1.69E-46
MS.gene051332	MS.gene56924	0.803762	2.99E-49	-1.69E-46
MS.gene051574	MS.gene56924	0.815971	7.03E-52	-1.69E-46
MS.gene051676	MS.gene56924	0.804312	2.30E-49	-1.69E-46
MS.gene051744	MS.gene56924	0.824582	7.47E-54	-1.69E-46
MS.gene051832	MS.gene56924	0.81391	2.02E-51	-1.69E-46
MS.gene051975	MS.gene56924	0.807032	6.16E-50	-1.69E-46
MS.gene052108	MS.gene56924	0.803111	4.07E-49	-1.69E-46
MS.gene052239	MS.gene56924	-0.820288	7.42E-53	-1.69E-46
MS.gene052519	MS.gene56924	0.816653	4.95E-52	-1.69E-46
MS.gene052533	MS.gene56924	0.801396	9.18E-49	-1.69E-46
MS.gene052582	MS.gene56924	0.847657	1.01E-59	-1.69E-46
MS.gene052584	MS.gene56924	0.848531	5.78E-60	-1.69E-46
MS.gene053006	MS.gene56924	0.845657	3.54E-59	-1.69E-46
MS.gene053226	MS.gene56924	0.829949	3.87E-55	-1.69E-46
MS.gene05365	MS.gene56924	0.818128	2.30E-52	-1.69E-46
MS.gene053931	MS.gene56924	0.814925	1.20E-51	-1.69E-46
MS.gene053963	MS.gene56924	0.806438	8.23E-50	-1.69E-46
MS.gene053995	MS.gene56924	0.820036	8.48E-53	-1.69E-46
MS.gene05415	MS.gene56924	0.806559	7.76E-50	-1.69E-46
MS.gene054222	MS.gene56924	0.82409	9.74E-54	-1.69E-46
MS.gene054576	MS.gene56924	0.812664	3.78E-51	-1.69E-46
MS.gene054651	MS.gene56924	0.843889	1.06E-58	-1.69E-46
MS.gene054775	MS.gene56924	0.80608	9.78E-50	-1.69E-46
MS.gene05548	MS.gene56924	0.808914	2.45E-50	-1.69E-46
MS.gene055555	MS.gene56924	0.804459	2.14E-49	-1.69E-46
MS.gene055586	MS.gene56924	0.819908	9.07E-53	-1.69E-46
MS.gene055778	MS.gene56924	0.806216	9.16E-50	-1.69E-46
MS.gene055808	MS.gene56924	0.815236	1.03E-51	-1.69E-46
MS.gene055818	MS.gene56924	0.812108	5.01E-51	-1.69E-46
MS.gene055841	MS.gene56924	0.859591	3.72E-63	-1.69E-46
MS.gene056091	MS.gene56924	0.822073	2.88E-53	-1.69E-46
MS.gene056366	MS.gene56924	0.830047	3.66E-55	-1.69E-46
MS.gene056699	MS.gene56924	0.801347	9.40E-49	-1.69E-46
MS.gene056776	MS.gene56924	0.829606	4.69E-55	-1.69E-46
MS.gene05686	MS.gene56924	0.81754	3.13E-52	-1.69E-46
MS.gene057060	MS.gene56924	0.816657	4.94E-52	-1.69E-46
MS.gene057102	MS.gene56924	0.826816	2.20E-54	-1.69E-46
MS.gene057208	MS.gene56924	0.847971	8.25E-60	-1.69E-46
MS.gene057444	MS.gene56924	0.808628	2.82E-50	-1.69E-46
MS.gene057693	MS.gene56924	0.801372	9.29E-49	-1.69E-46
MS.gene057705	MS.gene56924	0.821462	3.99E-53	-1.69E-46
MS.gene058037	MS.gene56924	0.822484	2.31E-53	-1.69E-46
MS.gene058279	MS.gene56924	0.82851	8.64E-55	-1.69E-46
MS.gene058625	MS.gene56924	0.825611	4.27E-54	-1.69E-46
MS.gene058783	MS.gene56924	0.80019	1.62E-48	-1.69E-46
MS.gene05950	MS.gene56924	0.810983	8.79E-51	-1.69E-46
MS.gene05953	MS.gene56924	0.830422	2.96E-55	-1.69E-46
MS.gene05954	MS.gene56924	0.855931	4.53E-62	-1.69E-46
MS.gene059625	MS.gene56924	0.810412	1.17E-50	-1.69E-46
MS.gene05985	MS.gene56924	-0.821782	3.36E-53	-1.69E-46
MS.gene059935	MS.gene56924	0.818803	1.62E-52	-1.69E-46
MS.gene060365	MS.gene56924	0.833622	4.80E-56	-1.69E-46
MS.gene060429	MS.gene56924	0.834877	2.32E-56	-1.69E-46
MS.gene060521	MS.gene56924	0.837117	6.28E-57	-1.69E-46
MS.gene060572	MS.gene56924	0.805369	1.38E-49	-1.69E-46
MS.gene060634	MS.gene56924	-0.818601	1.80E-52	-1.69E-46
MS.gene060879	MS.gene56924	0.802603	5.19E-49	-1.69E-46
MS.gene060935	MS.gene56924	0.803593	3.24E-49	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene58134	MS.gene56924	PPI
MS.gene02265	MS.gene56924	PPI
MS.gene56924	MS.gene058061	PPI
MS.gene56924	MS.gene012127	PPI
MS.gene28183	MS.gene56924	PPI
MS.gene56924	MS.gene21801	PPI
MS.gene56924	MS.gene24375	PPI
MS.gene06729	MS.gene56924	PPI
MS.gene56924	MS.gene58136	PPI
MS.gene014393	MS.gene56924	PPI
MS.gene044013	MS.gene56924	PPI
MS.gene44230	MS.gene56924	PPI
MS.gene56924	MS.gene033335	PPI
MS.gene56924	MS.gene59485	PPI
MS.gene56924	MS.gene002648	PPI
MS.gene062770	MS.gene56924	PPI
MS.gene025808	MS.gene56924	PPI
MS.gene072220	MS.gene56924	PPI
MS.gene40486	MS.gene56924	PPI
MS.gene06946	MS.gene56924	PPI
MS.gene56924	MS.gene48281	PPI
MS.gene56924	MS.gene057144	PPI
MS.gene56924	MS.gene064786	PPI
MS.gene030686	MS.gene56924	PPI
MS.gene011376	MS.gene56924	PPI
MS.gene56924	MS.gene004328	PPI
MS.gene28454	MS.gene56924	PPI
MS.gene24542	MS.gene56924	PPI
MS.gene32974	MS.gene56924	PPI
MS.gene56924	MS.gene52815	PPI
MS.gene011065	MS.gene56924	PPI
MS.gene051630	MS.gene56924	PPI
MS.gene67460	MS.gene56924	PPI
MS.gene01284	MS.gene56924	PPI
MS.gene56924	MS.gene69373	PPI
MS.gene56924	MS.gene70283	PPI
MS.gene67099	MS.gene56924	PPI
MS.gene56924	MS.gene70282	PPI
MS.gene013607	MS.gene56924	PPI
MS.gene09974	MS.gene56924	PPI
MS.gene48400	MS.gene56924	PPI
MS.gene030685	MS.gene56924	PPI
MS.gene48279	MS.gene56924	PPI
MS.gene42312	MS.gene56924	PPI
MS.gene014485	MS.gene56924	PPI
MS.gene57218	MS.gene56924	PPI
MS.gene56924	MS.gene64789	PPI
MS.gene072221	MS.gene56924	PPI
MS.gene56924	MS.gene36633	PPI
MS.gene56924	MS.gene024828	PPI
MS.gene56924	MS.gene01697	PPI
MS.gene69461	MS.gene56924	PPI
MS.gene56924	MS.gene057145	PPI
MS.gene064694	MS.gene56924	PPI
MS.gene017622	MS.gene56924	PPI
MS.gene56924	MS.gene36746	PPI
MS.gene89672	MS.gene56924	PPI
MS.gene002306	MS.gene56924	PPI
MS.gene68079	MS.gene56924	PPI
MS.gene56924	MS.gene21169	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 17 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGACGTTCTCGGAAGGTTT+TGG	0.276388	8.1:+18619523	None:intergenic
CTCAGCAATGGGAAGATGAT+TGG	0.389575	8.1:-18619268	MS.gene56924:CDS
TCAGCTAAAGAGAGAGTTAG+AGG	0.407568	8.1:-18619213	MS.gene56924:CDS
GAGATCAACCTTGACGTTCT+CGG	0.418361	8.1:+18619513	None:intergenic
AGACAAGGAGGAAGGGAGAG+AGG	0.431303	8.1:-18619294	MS.gene56924:CDS
TGAAGACGACGACGAGTTCG+AGG	0.463513	8.1:-18619488	MS.gene56924:CDS
TCAGCAATGGGAAGATGATT+GGG	0.477084	8.1:-18619267	MS.gene56924:CDS
GGAGAGAGGTAGCTCAGCAA+TGG	0.520450	8.1:-18619280	MS.gene56924:CDS
GGTTGGGAAGACAAGGAGGA+AGG	0.522923	8.1:-18619302	MS.gene56924:CDS
GAGAGAGGTAGCTCAGCAAT+GGG	0.525728	8.1:-18619279	MS.gene56924:CDS
GTTGGGAAGACAAGGAGGAA+GGG	0.529450	8.1:-18619301	MS.gene56924:CDS
TCAACCTTGACGTTCTCGGA+AGG	0.583809	8.1:+18619517	None:intergenic
ACTTGCAGGTTGGGAAGACA+AGG	0.596138	8.1:-18619309	MS.gene56924:intron
CAGCTAAAGAGAGAGTTAGA+GGG	0.638489	8.1:-18619212	MS.gene56924:CDS
GAGGAGTTTGAAATCAACGA+AGG	0.657612	8.1:-18619469	MS.gene56924:intron
TGCAGGTTGGGAAGACAAGG+AGG	0.659645	8.1:-18619306	MS.gene56924:intron
AAAACCTTCCGAGAACGTCA+AGG	0.660047	8.1:-18619521	MS.gene56924:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TACTAACATGAATTCAAATC+TGG	+	chr8.1:18619366-18619385	None:intergenic	25.0%
	AAATTGATTGCAACAACGAT+TGG	+	chr8.1:18619323-18619342	None:intergenic	30.0%
	AACAACGATTGGAATGAAGA+GGG	+	chr8.1:18619312-18619331	None:intergenic	35.0%
!	ATCTTATGTAACTTGCAGGT+TGG	-	chr8.1:18619409-18619428	MS.gene56924:intron	35.0%
!	TCTTATGTAACTTGCAGGTT+GGG	-	chr8.1:18619410-18619429	MS.gene56924:intron	35.0%
!	TGCAATCTTATGTAACTTGC+AGG	-	chr8.1:18619405-18619424	MS.gene56924:intron	35.0%
	AATGAAGAGGGGTTAAAGGT+TGG	+	chr8.1:18619300-18619319	None:intergenic	40.0%
	ACAACGATTGGAATGAAGAG+GGG	+	chr8.1:18619311-18619330	None:intergenic	40.0%
	CAACAACGATTGGAATGAAG+AGG	+	chr8.1:18619313-18619332	None:intergenic	40.0%
	CAGCTAAAGAGAGAGTTAGA+GGG	-	chr8.1:18619516-18619535	MS.gene56924:CDS	40.0%
	TCAGCAATGGGAAGATGATT+GGG	-	chr8.1:18619461-18619480	MS.gene56924:intron	40.0%
	TCAGCTAAAGAGAGAGTTAG+AGG	-	chr8.1:18619515-18619534	MS.gene56924:CDS	40.0%
	TTGGAATGAAGAGGGGTTAA+AGG	+	chr8.1:18619304-18619323	None:intergenic	40.0%
!	GAGGAGTTTGAAATCAACGA+AGG	-	chr8.1:18619259-18619278	MS.gene56924:CDS	40.0%
	AAAACCTTCCGAGAACGTCA+AGG	-	chr8.1:18619207-18619226	MS.gene56924:CDS	45.0%
	CTCAGCAATGGGAAGATGAT+TGG	-	chr8.1:18619460-18619479	MS.gene56924:intron	45.0%
	GAGATCAACCTTGACGTTCT+CGG	+	chr8.1:18619218-18619237	None:intergenic	45.0%
!	TTGACGTTCTCGGAAGGTTT+TGG	+	chr8.1:18619208-18619227	None:intergenic	45.0%
	GAGAGAGGTAGCTCAGCAAT+GGG	-	chr8.1:18619449-18619468	MS.gene56924:intron	50.0%
	GTTGGGAAGACAAGGAGGAA+GGG	-	chr8.1:18619427-18619446	MS.gene56924:intron	50.0%
	TCAACCTTGACGTTCTCGGA+AGG	+	chr8.1:18619214-18619233	None:intergenic	50.0%
!	ACTTGCAGGTTGGGAAGACA+AGG	-	chr8.1:18619419-18619438	MS.gene56924:intron	50.0%
!!!	GTTCTCGGAAGGTTTTGGCT+CGG	+	chr8.1:18619203-18619222	None:intergenic	50.0%
	AGACAAGGAGGAAGGGAGAG+AGG	-	chr8.1:18619434-18619453	MS.gene56924:intron	55.0%
	GGAGAGAGGTAGCTCAGCAA+TGG	-	chr8.1:18619448-18619467	MS.gene56924:intron	55.0%
	GGTTGGGAAGACAAGGAGGA+AGG	-	chr8.1:18619426-18619445	MS.gene56924:intron	55.0%
	TGAAGACGACGACGAGTTCG+AGG	-	chr8.1:18619240-18619259	MS.gene56924:CDS	55.0%
!	TGCAGGTTGGGAAGACAAGG+AGG	-	chr8.1:18619422-18619441	MS.gene56924:intron	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.1	gene	18619193	18619557	18619193	ID=MS.gene56924
chr8.1	mRNA	18619193	18619557	18619193	ID=MS.gene56924.t1;Parent=MS.gene56924
chr8.1	exon	18619470	18619557	18619470	ID=MS.gene56924.t1.exon1;Parent=MS.gene56924.t1
chr8.1	CDS	18619470	18619557	18619470	ID=cds.MS.gene56924.t1;Parent=MS.gene56924.t1
chr8.1	exon	18619193	18619323	18619193	ID=MS.gene56924.t1.exon2;Parent=MS.gene56924.t1
chr8.1	CDS	18619193	18619323	18619193	ID=cds.MS.gene56924.t1;Parent=MS.gene56924.t1

Gene Sequence	>MS.gene56924 ATGGCAACCGAGCCAAAACCTTCCGAGAACGTCAAGGTTGATCTCTTTGAAGACGACGACGAGTTCGAGGAGTTTGAAATCAACGAAGGTAACTTCAAATCGTTCCAACCTTTAACCCCTCTTCATTCCAATCGTTGTTGCAATCAATTTCAGCTTCATTCAATTTCGATCCAGATTTGAATTCATGTTAGTAGTTTCTTATTGTTTATTTTTGCAATCTTATGTAACTTGCAGGTTGGGAAGACAAGGAGGAAGGGAGAGAGGTAGCTCAGCAATGGGAAGATGATTGGGATGATGATGATGTTAGTGATGATTTCTCTCTTCAGCTAAAGAGAGAGTTAGAGGGCAATGCTGAGAAGAAATAA
Protein sequence	>MS.gene56924.t1 MATEPKPSENVKVDLFEDDDEFEEFEINEGWEDKEEGREVAQQWEDDWDDDDVSDDFSLQLKRELEGNAEKK