Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37233.t1 | XP_013457666.1 | 94.8 | 615 | 32 | 0 | 1 | 615 | 1 | 615 | 0.00E+00 | 1150.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37233.t1 | Q8LGM7 | 23.4 | 321 | 185 | 12 | 67 | 353 | 2 | 295 | 9.8e-11 | 70.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37233.t1 | A0A072UPS3 | 94.8 | 615 | 32 | 0 | 1 | 615 | 1 | 615 | 0.0e+00 | 1150.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04933 | MS.gene37233 | 0.804926 | 1.71E-49 | -1.69E-46 |
| MS.gene049346 | MS.gene37233 | 0.814819 | 1.27E-51 | -1.69E-46 |
| MS.gene049488 | MS.gene37233 | 0.80324 | 3.83E-49 | -1.69E-46 |
| MS.gene049681 | MS.gene37233 | 0.814612 | 1.41E-51 | -1.69E-46 |
| MS.gene049993 | MS.gene37233 | 0.886339 | 3.73E-72 | -1.69E-46 |
| MS.gene050301 | MS.gene37233 | 0.803237 | 3.84E-49 | -1.69E-46 |
| MS.gene050397 | MS.gene37233 | 0.853214 | 2.77E-61 | -1.69E-46 |
| MS.gene050406 | MS.gene37233 | 0.811793 | 5.86E-51 | -1.69E-46 |
| MS.gene050426 | MS.gene37233 | -0.854015 | 1.63E-61 | -1.69E-46 |
| MS.gene050464 | MS.gene37233 | 0.833603 | 4.85E-56 | -1.69E-46 |
| MS.gene050529 | MS.gene37233 | 0.811636 | 6.34E-51 | -1.69E-46 |
| MS.gene050642 | MS.gene37233 | 0.805375 | 1.38E-49 | -1.69E-46 |
| MS.gene050650 | MS.gene37233 | 0.810734 | 9.95E-51 | -1.69E-46 |
| MS.gene050670 | MS.gene37233 | 0.85104 | 1.15E-60 | -1.69E-46 |
| MS.gene051525 | MS.gene37233 | 0.869957 | 2.10E-66 | -1.69E-46 |
| MS.gene051582 | MS.gene37233 | 0.828208 | 1.02E-54 | -1.69E-46 |
| MS.gene051860 | MS.gene37233 | 0.833161 | 6.25E-56 | -1.69E-46 |
| MS.gene051983 | MS.gene37233 | 0.80795 | 3.94E-50 | -1.69E-46 |
| MS.gene052011 | MS.gene37233 | -0.9275 | 1.08E-91 | -1.69E-46 |
| MS.gene052077 | MS.gene37233 | 0.810721 | 1.00E-50 | -1.69E-46 |
| MS.gene052128 | MS.gene37233 | -0.811114 | 8.23E-51 | -1.69E-46 |
| MS.gene052223 | MS.gene37233 | -0.803142 | 4.01E-49 | -1.69E-46 |
| MS.gene052253 | MS.gene37233 | 0.868731 | 5.25E-66 | -1.69E-46 |
| MS.gene052340 | MS.gene37233 | 0.810296 | 1.24E-50 | -1.69E-46 |
| MS.gene052481 | MS.gene37233 | 0.800177 | 1.63E-48 | -1.69E-46 |
| MS.gene052589 | MS.gene37233 | 0.802616 | 5.16E-49 | -1.69E-46 |
| MS.gene053330 | MS.gene37233 | 0.808159 | 3.55E-50 | -1.69E-46 |
| MS.gene053836 | MS.gene37233 | 0.843479 | 1.37E-58 | -1.69E-46 |
| MS.gene053873 | MS.gene37233 | 0.836795 | 7.59E-57 | -1.69E-46 |
| MS.gene054010 | MS.gene37233 | 0.800307 | 1.53E-48 | -1.69E-46 |
| MS.gene054011 | MS.gene37233 | 0.829924 | 3.92E-55 | -1.69E-46 |
| MS.gene054012 | MS.gene37233 | 0.824247 | 8.95E-54 | -1.69E-46 |
| MS.gene054013 | MS.gene37233 | 0.818444 | 1.95E-52 | -1.69E-46 |
| MS.gene054102 | MS.gene37233 | 0.815853 | 7.47E-52 | -1.69E-46 |
| MS.gene054173 | MS.gene37233 | 0.810471 | 1.13E-50 | -1.69E-46 |
| MS.gene054183 | MS.gene37233 | 0.886969 | 2.15E-72 | -1.69E-46 |
| MS.gene054349 | MS.gene37233 | 0.815915 | 7.24E-52 | -1.69E-46 |
| MS.gene054473 | MS.gene37233 | 0.827778 | 1.30E-54 | -1.69E-46 |
| MS.gene054556 | MS.gene37233 | 0.800148 | 1.65E-48 | -1.69E-46 |
| MS.gene054719 | MS.gene37233 | 0.843121 | 1.70E-58 | -1.69E-46 |
| MS.gene054918 | MS.gene37233 | 0.805375 | 1.38E-49 | -1.69E-46 |
| MS.gene054920 | MS.gene37233 | 0.804963 | 1.68E-49 | -1.69E-46 |
| MS.gene054983 | MS.gene37233 | 0.842238 | 2.92E-58 | -1.69E-46 |
| MS.gene055116 | MS.gene37233 | 0.840423 | 8.78E-58 | -1.69E-46 |
| MS.gene055217 | MS.gene37233 | 0.817366 | 3.42E-52 | -1.69E-46 |
| MS.gene055358 | MS.gene37233 | 0.804295 | 2.31E-49 | -1.69E-46 |
| MS.gene055369 | MS.gene37233 | 0.808909 | 2.46E-50 | -1.69E-46 |
| MS.gene055428 | MS.gene37233 | 0.824712 | 6.96E-54 | -1.69E-46 |
| MS.gene05553 | MS.gene37233 | 0.819916 | 9.03E-53 | -1.69E-46 |
| MS.gene055601 | MS.gene37233 | -0.807112 | 5.93E-50 | -1.69E-46 |
| MS.gene055604 | MS.gene37233 | 0.816251 | 6.09E-52 | -1.69E-46 |
| MS.gene05594 | MS.gene37233 | 0.831277 | 1.83E-55 | -1.69E-46 |
| MS.gene056191 | MS.gene37233 | 0.832643 | 8.41E-56 | -1.69E-46 |
| MS.gene056330 | MS.gene37233 | -0.820718 | 5.91E-53 | -1.69E-46 |
| MS.gene056337 | MS.gene37233 | 0.829188 | 5.92E-55 | -1.69E-46 |
| MS.gene056407 | MS.gene37233 | 0.828704 | 7.75E-55 | -1.69E-46 |
| MS.gene056514 | MS.gene37233 | 0.828843 | 7.18E-55 | -1.69E-46 |
| MS.gene056612 | MS.gene37233 | 0.801105 | 1.05E-48 | -1.69E-46 |
| MS.gene056659 | MS.gene37233 | 0.819336 | 1.23E-52 | -1.69E-46 |
| MS.gene056723 | MS.gene37233 | 0.836109 | 1.13E-56 | -1.69E-46 |
| MS.gene056899 | MS.gene37233 | -0.847942 | 8.40E-60 | -1.69E-46 |
| MS.gene056920 | MS.gene37233 | -0.801051 | 1.08E-48 | -1.69E-46 |
| MS.gene056924 | MS.gene37233 | -0.802242 | 6.16E-49 | -1.69E-46 |
| MS.gene056979 | MS.gene37233 | 0.800991 | 1.11E-48 | -1.69E-46 |
| MS.gene05702 | MS.gene37233 | 0.841088 | 5.87E-58 | -1.69E-46 |
| MS.gene057121 | MS.gene37233 | 0.808635 | 2.81E-50 | -1.69E-46 |
| MS.gene057476 | MS.gene37233 | 0.891527 | 3.66E-74 | -1.69E-46 |
| MS.gene057749 | MS.gene37233 | 0.856783 | 2.55E-62 | -1.69E-46 |
| MS.gene057823 | MS.gene37233 | 0.848489 | 5.93E-60 | -1.69E-46 |
| MS.gene057825 | MS.gene37233 | 0.81135 | 7.32E-51 | -1.69E-46 |
| MS.gene057826 | MS.gene37233 | 0.805143 | 1.54E-49 | -1.69E-46 |
| MS.gene057833 | MS.gene37233 | 0.837961 | 3.82E-57 | -1.69E-46 |
| MS.gene057843 | MS.gene37233 | 0.832412 | 9.59E-56 | -1.69E-46 |
| MS.gene057888 | MS.gene37233 | 0.866337 | 3.07E-65 | -1.69E-46 |
| MS.gene058002 | MS.gene37233 | 0.819644 | 1.04E-52 | -1.69E-46 |
| MS.gene05811 | MS.gene37233 | 0.816999 | 4.14E-52 | -1.69E-46 |
| MS.gene058142 | MS.gene37233 | 0.810235 | 1.27E-50 | -1.69E-46 |
| MS.gene058233 | MS.gene37233 | 0.80886 | 2.52E-50 | -1.69E-46 |
| MS.gene058444 | MS.gene37233 | 0.815646 | 8.31E-52 | -1.69E-46 |
| MS.gene058660 | MS.gene37233 | 0.80157 | 8.46E-49 | -1.69E-46 |
| MS.gene058710 | MS.gene37233 | 0.81385 | 2.08E-51 | -1.69E-46 |
| MS.gene05886 | MS.gene37233 | 0.834404 | 3.06E-56 | -1.69E-46 |
| MS.gene058885 | MS.gene37233 | 0.820039 | 8.47E-53 | -1.69E-46 |
| MS.gene059011 | MS.gene37233 | 0.821816 | 3.30E-53 | -1.69E-46 |
| MS.gene05907 | MS.gene37233 | 0.848142 | 7.40E-60 | -1.69E-46 |
| MS.gene059082 | MS.gene37233 | 0.829957 | 3.85E-55 | -1.69E-46 |
| MS.gene059141 | MS.gene37233 | 0.818821 | 1.60E-52 | -1.69E-46 |
| MS.gene059285 | MS.gene37233 | 0.808093 | 3.67E-50 | -1.69E-46 |
| MS.gene059294 | MS.gene37233 | 0.814023 | 1.90E-51 | -1.69E-46 |
| MS.gene059420 | MS.gene37233 | 0.887623 | 1.21E-72 | -1.69E-46 |
| MS.gene059462 | MS.gene37233 | 0.835402 | 1.71E-56 | -1.69E-46 |
| MS.gene05984 | MS.gene37233 | 0.837395 | 5.33E-57 | -1.69E-46 |
| MS.gene059876 | MS.gene37233 | 0.80965 | 1.70E-50 | -1.69E-46 |
| MS.gene060038 | MS.gene37233 | 0.837838 | 4.10E-57 | -1.69E-46 |
| MS.gene060403 | MS.gene37233 | 0.82135 | 4.23E-53 | -1.69E-46 |
| MS.gene061049 | MS.gene37233 | 0.841959 | 3.46E-58 | -1.69E-46 |
| MS.gene061114 | MS.gene37233 | -0.828871 | 7.06E-55 | -1.69E-46 |
| MS.gene061154 | MS.gene37233 | 0.808899 | 2.47E-50 | -1.69E-46 |
| MS.gene061214 | MS.gene37233 | 0.821383 | 4.16E-53 | -1.69E-46 |
| MS.gene061215 | MS.gene37233 | 0.823025 | 1.73E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37233.t1 | MTR_4g102620 | 94.797 | 615 | 32 | 0 | 1 | 615 | 1 | 615 | 0.0 | 1194 |
| MS.gene37233.t1 | MTR_1g101790 | 50.155 | 646 | 270 | 14 | 12 | 615 | 8 | 643 | 0.0 | 597 |
| MS.gene37233.t1 | MTR_1g101790 | 49.142 | 641 | 274 | 14 | 12 | 610 | 8 | 638 | 0.0 | 577 |
| MS.gene37233.t1 | MTR_6g089580 | 47.604 | 647 | 279 | 15 | 6 | 615 | 5 | 628 | 0.0 | 558 |
| MS.gene37233.t1 | MTR_6g464840 | 47.759 | 647 | 278 | 15 | 6 | 615 | 5 | 628 | 0.0 | 558 |
| MS.gene37233.t1 | MTR_8g079020 | 36.723 | 354 | 190 | 8 | 31 | 370 | 68 | 401 | 1.56e-71 | 248 |
| MS.gene37233.t1 | MTR_8g079020 | 40.000 | 205 | 115 | 5 | 398 | 598 | 725 | 925 | 1.67e-35 | 143 |
| MS.gene37233.t1 | MTR_8g079040 | 37.309 | 327 | 179 | 6 | 50 | 370 | 95 | 401 | 2.86e-71 | 248 |
| MS.gene37233.t1 | MTR_8g079040 | 38.725 | 204 | 119 | 4 | 398 | 598 | 721 | 921 | 3.31e-35 | 142 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37233.t1 | AT5G51920 | 44.209 | 613 | 276 | 12 | 1 | 604 | 1 | 556 | 2.00e-165 | 484 |
| MS.gene37233.t1 | AT4G22980 | 39.409 | 609 | 301 | 12 | 10 | 610 | 9 | 557 | 4.80e-136 | 409 |
| MS.gene37233.t1 | AT5G66950 | 40.367 | 327 | 170 | 6 | 50 | 370 | 93 | 400 | 5.55e-75 | 257 |
| MS.gene37233.t1 | AT5G66950 | 42.703 | 185 | 91 | 4 | 421 | 600 | 681 | 855 | 2.95e-35 | 142 |
| MS.gene37233.t1 | AT2G23520 | 36.769 | 359 | 201 | 7 | 19 | 370 | 63 | 402 | 1.33e-73 | 253 |
| MS.gene37233.t1 | AT2G23520 | 37.438 | 203 | 116 | 4 | 406 | 597 | 674 | 876 | 3.74e-30 | 127 |
| MS.gene37233.t1 | AT4G37100 | 36.612 | 366 | 196 | 9 | 63 | 421 | 110 | 446 | 3.05e-73 | 252 |
| MS.gene37233.t1 | AT4G37100 | 41.808 | 177 | 96 | 4 | 423 | 597 | 704 | 875 | 9.72e-34 | 138 |
| MS.gene37233.t1 | AT1G16540 | 21.793 | 569 | 310 | 23 | 75 | 589 | 4 | 491 | 3.62e-13 | 73.2 |
| MS.gene37233.t1 | AT1G16540 | 21.793 | 569 | 310 | 23 | 75 | 589 | 4 | 491 | 6.27e-13 | 72.4 |
| MS.gene37233.t1 | AT1G16540 | 21.793 | 569 | 310 | 23 | 75 | 589 | 4 | 491 | 6.94e-13 | 72.4 |
Find 91 sgRNAs with CRISPR-Local
Find 114 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAACAAGATATTTGATTAAT+TGG | 0.155783 | 8.3:+19155098 | MS.gene37233:CDS |
| TCTCCATTCAATTCCTAAAT+TGG | 0.170825 | 8.3:-19154486 | None:intergenic |
| ATTTCTTCACTTAAAGATTT+AGG | 0.176964 | 8.3:+19154794 | MS.gene37233:CDS |
| CCGTCAAACTCTGGATTATT+AGG | 0.217004 | 8.3:+19154902 | MS.gene37233:CDS |
| CCACTTTATTCAAAGGTTAC+TGG | 0.222847 | 8.3:+19154569 | MS.gene37233:CDS |
| CAAACTATGAAGATGTTTAT+AGG | 0.228960 | 8.3:+19155479 | MS.gene37233:CDS |
| GAATTCACATGGTCCAATTT+AGG | 0.230968 | 8.3:+19154473 | MS.gene37233:CDS |
| CCTAAAGTGTCTCTTTCTTT+TGG | 0.243729 | 8.3:-19154671 | None:intergenic |
| ATGAAGATGTTTATAGGTTA+TGG | 0.244282 | 8.3:+19155485 | MS.gene37233:CDS |
| AGGCCAATTCCCTCTCTTCT+TGG | 0.281938 | 8.3:-19155351 | None:intergenic |
| GAAGATGATAACATGTAAAA+AGG | 0.297027 | 8.3:+19154517 | MS.gene37233:CDS |
| TGAAGATGTTTATAGGTTAT+GGG | 0.323284 | 8.3:+19155486 | MS.gene37233:CDS |
| TGTGAGAAGCTCTGATCCTT+TGG | 0.342674 | 8.3:-19154372 | None:intergenic |
| AAAATATCAAGTTCTTTGAT+AGG | 0.352408 | 8.3:+19155326 | MS.gene37233:CDS |
| TGGAAAACAACCATTGAAAC+AGG | 0.356867 | 8.3:-19153787 | None:intergenic |
| ATGTCTTTGGCACAAGAAAA+TGG | 0.362723 | 8.3:+19154614 | MS.gene37233:CDS |
| GGTGAAAATCCATCTGGATT+TGG | 0.362913 | 8.3:+19154746 | MS.gene37233:CDS |
| CTCAAATGAGTATAGGATAT+TGG | 0.368511 | 8.3:+19155429 | MS.gene37233:CDS |
| AAAATGCAACCACCATGTTC+TGG | 0.372071 | 8.3:+19153747 | None:intergenic |
| TTTACTGACACTATTTGAAT+TGG | 0.373946 | 8.3:-19153850 | None:intergenic |
| GCAAATGAAGTTGAAAAGTT+AGG | 0.395110 | 8.3:+19155385 | MS.gene37233:CDS |
| GTCTTAACCATCTCGTTCTC+AGG | 0.402171 | 8.3:-19154935 | None:intergenic |
| AGTGTAATCAAAACATGTAT+TGG | 0.413697 | 8.3:-19154057 | None:intergenic |
| AATGAAAACTAAGGCGTAAT+CGG | 0.417763 | 8.3:-19154297 | None:intergenic |
| CCAGTAACCTTTGAATAAAG+TGG | 0.420797 | 8.3:-19154569 | None:intergenic |
| ATCTTTGGTGAAAATCCATC+TGG | 0.432099 | 8.3:+19154740 | MS.gene37233:CDS |
| GTAAATGTGGGAGAGATTCA+TGG | 0.432348 | 8.3:-19153932 | None:intergenic |
| TTGAATTGGAAGTGTTATCA+TGG | 0.434250 | 8.3:-19153836 | None:intergenic |
| TCATCACAACCCTGTTTCAA+TGG | 0.435276 | 8.3:+19153777 | MS.gene37233:CDS |
| GGAGATGAATGATGAAAGAT+TGG | 0.441255 | 8.3:+19155015 | MS.gene37233:CDS |
| TGAGAAGTTGTGGAAAATTG+AGG | 0.444472 | 8.3:-19153987 | None:intergenic |
| AGAAGAGAGGGAATTGGCCT+TGG | 0.450623 | 8.3:+19155354 | MS.gene37233:CDS |
| AATGAACAAACCATAAAATC+AGG | 0.451423 | 8.3:-19154710 | None:intergenic |
| GGTCCAATTTAGGAATTGAA+TGG | 0.456840 | 8.3:+19154483 | MS.gene37233:CDS |
| TTCATTTGCTTTAGTTTCCA+AGG | 0.465141 | 8.3:-19155371 | None:intergenic |
| TTGATCTACTTGAGAAGTTG+TGG | 0.473181 | 8.3:-19153997 | None:intergenic |
| GAGAAGTTGTGGAAAATTGA+GGG | 0.473696 | 8.3:-19153986 | None:intergenic |
| TGGTTTGTGAAATGTGTGTT+GGG | 0.475864 | 8.3:-19153912 | None:intergenic |
| GAACCAAGAAGAGAGGGAAT+TGG | 0.478067 | 8.3:+19155348 | MS.gene37233:CDS |
| AGCTCCACAATTTCTGATGA+TGG | 0.487761 | 8.3:-19154983 | None:intergenic |
| CCTAATAATCCAGAGTTTGA+CGG | 0.487873 | 8.3:-19154902 | None:intergenic |
| CCAAAAGAAAGAGACACTTT+AGG | 0.489254 | 8.3:+19154671 | MS.gene37233:CDS |
| GAGTTCTCAGAGGCAAGAAA+TGG | 0.491382 | 8.3:-19153813 | None:intergenic |
| TGATCATAGCAATGAGGCAT+TGG | 0.493736 | 8.3:+19154400 | MS.gene37233:CDS |
| TTTCATTAATTAGAATCTAT+GGG | 0.496665 | 8.3:+19155170 | MS.gene37233:CDS |
| GAGAGTGCACCGTCAAACTC+TGG | 0.498403 | 8.3:+19154893 | MS.gene37233:CDS |
| CTCATTAGCAATGAAAACTA+AGG | 0.499860 | 8.3:-19154306 | None:intergenic |
| TGGCATTTAATATATTTGAC+TGG | 0.501389 | 8.3:+19155224 | MS.gene37233:CDS |
| TTTCCACAAAATCAGCATCA+AGG | 0.507755 | 8.3:-19155525 | None:intergenic |
| ATGGTTTGTGAAATGTGTGT+TGG | 0.508371 | 8.3:-19153913 | None:intergenic |
| TTTAATATATTTGACTGGAA+AGG | 0.511917 | 8.3:+19155229 | MS.gene37233:CDS |
| TTGTGCAGAAGTTAGCTGAT+AGG | 0.513041 | 8.3:+19155272 | MS.gene37233:CDS |
| GGAATAATATCTCTCTAAGC+AGG | 0.516126 | 8.3:+19155293 | MS.gene37233:CDS |
| GCTAACTTCTGCACAAGTGT+TGG | 0.523200 | 8.3:-19155265 | None:intergenic |
| TTATATGAGTCTTGTAAATG+TGG | 0.525845 | 8.3:-19153945 | None:intergenic |
| AAAGTGGCTCAAATGAGTAT+AGG | 0.526368 | 8.3:+19155422 | MS.gene37233:CDS |
| TCATTTCTATTTCAATCCAA+AGG | 0.532256 | 8.3:+19154356 | MS.gene37233:CDS |
| TATATGAGTCTTGTAAATGT+GGG | 0.535373 | 8.3:-19153944 | None:intergenic |
| TTTAGGAAATGCTACAAGCA+TGG | 0.536864 | 8.3:+19154811 | MS.gene37233:CDS |
| CTAGTTCTGATTCTTGTCCT+CGG | 0.537895 | 8.3:-19154226 | None:intergenic |
| TTAGGAAATGCTACAAGCAT+GGG | 0.540646 | 8.3:+19154812 | MS.gene37233:CDS |
| TGAATTGGAAGTGTTATCAT+GGG | 0.542936 | 8.3:-19153835 | None:intergenic |
| ATCACCATCATCAGAAATTG+TGG | 0.551638 | 8.3:+19154979 | MS.gene37233:CDS |
| AATATATTAAATGCCACTGT+AGG | 0.551745 | 8.3:-19155217 | None:intergenic |
| AATTGTGGAGCTACAAGGCA+TGG | 0.566632 | 8.3:+19154994 | MS.gene37233:CDS |
| TCAGAAATTGTGGAGCTACA+AGG | 0.567806 | 8.3:+19154989 | MS.gene37233:CDS |
| GGCATGTCCAAGAAGAAAAG+TGG | 0.570932 | 8.3:+19155406 | MS.gene37233:CDS |
| AAGACTTGAAGATGTGGCTG+AGG | 0.577054 | 8.3:-19153886 | None:intergenic |
| TTGATAGGAACCAAGAAGAG+AGG | 0.577292 | 8.3:+19155341 | MS.gene37233:CDS |
| CGCTTCTCCTGAGAACGAGA+TGG | 0.584335 | 8.3:+19154928 | MS.gene37233:CDS |
| TGATAGGAACCAAGAAGAGA+GGG | 0.586492 | 8.3:+19155342 | MS.gene37233:CDS |
| AATAAACAACCAAATCCAGA+TGG | 0.590992 | 8.3:-19154755 | None:intergenic |
| TCAGAGGCAAGAAATGGAGA+TGG | 0.596800 | 8.3:-19153807 | None:intergenic |
| GACTAAAGAAGAGCAAAATG+AGG | 0.605972 | 8.3:+19154955 | MS.gene37233:CDS |
| CTTTGGCACAAGAAAATGGT+TGG | 0.615852 | 8.3:+19154618 | MS.gene37233:CDS |
| ATTGATAGTTGCAAGAAGCA+AGG | 0.622439 | 8.3:+19154431 | MS.gene37233:CDS |
| GTGTGACATTGTGCAAATGT+AGG | 0.624324 | 8.3:-19154851 | None:intergenic |
| TTAGACCACGCAGACTCAGT+GGG | 0.643409 | 8.3:+19155055 | MS.gene37233:CDS |
| GATGATGTTCCTCCAGAACA+TGG | 0.646154 | 8.3:-19153759 | None:intergenic |
| TTCACATCGTGGACCTACAG+TGG | 0.650419 | 8.3:+19155204 | MS.gene37233:CDS |
| AGATTGGAAATTGAGTGTAG+AGG | 0.650792 | 8.3:+19155031 | MS.gene37233:CDS |
| ATTAGACCACGCAGACTCAG+TGG | 0.651262 | 8.3:+19155054 | MS.gene37233:CDS |
| ATGCAACCACCATGTTCTGG+AGG | 0.655118 | 8.3:+19153750 | MS.gene37233:CDS |
| CAAGTAGCGAGTAGACGACG+AGG | 0.680933 | 8.3:-19154138 | None:intergenic |
| TTAGTCCCACTGAGTCTGCG+TGG | 0.687077 | 8.3:-19155060 | None:intergenic |
| AGTGTATGATCATAGCAATG+AGG | 0.687969 | 8.3:+19154394 | MS.gene37233:CDS |
| GGGATAAAGACTTGAAGATG+TGG | 0.694505 | 8.3:-19153892 | None:intergenic |
| GGAAAACAACCATTGAAACA+GGG | 0.697784 | 8.3:-19153786 | None:intergenic |
| GTTATCATGGGAGTTCTCAG+AGG | 0.702910 | 8.3:-19153823 | None:intergenic |
| AAGTCACAGATACTCTACCG+AGG | 0.761105 | 8.3:+19154209 | MS.gene37233:CDS |
| GATGTTCCTCCAGAACATGG+TGG | 0.790301 | 8.3:-19153756 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTCATTAATTAGAATCTAT+GGG | + | chr8.3:19155170-19155189 | MS.gene37233:CDS | 15.0% |
| !!! | TTTTCATTAATTAGAATCTA+TGG | + | chr8.3:19155169-19155188 | MS.gene37233:CDS | 15.0% |
| !! | AAAATATCAAGTTCTTTGAT+AGG | + | chr8.3:19155326-19155345 | MS.gene37233:CDS | 20.0% |
| !! | ATTTCTTCACTTAAAGATTT+AGG | + | chr8.3:19154794-19154813 | MS.gene37233:CDS | 20.0% |
| !! | GAACAAGATATTTGATTAAT+TGG | + | chr8.3:19155098-19155117 | MS.gene37233:CDS | 20.0% |
| !! | TTTAATATATTTGACTGGAA+AGG | + | chr8.3:19155229-19155248 | MS.gene37233:CDS | 20.0% |
| !!! | ATGAATAAGAACATGATTTT+TGG | - | chr8.3:19154115-19154134 | None:intergenic | 20.0% |
| !!! | TATGTTTAAACCTGATTTTA+TGG | + | chr8.3:19154700-19154719 | MS.gene37233:CDS | 20.0% |
| !!! | TGTTCATTTTACAAAATCTT+TGG | + | chr8.3:19154725-19154744 | MS.gene37233:CDS | 20.0% |
| ! | AATATATTAAATGCCACTGT+AGG | - | chr8.3:19155220-19155239 | None:intergenic | 25.0% |
| ! | AATGAACAAACCATAAAATC+AGG | - | chr8.3:19154713-19154732 | None:intergenic | 25.0% |
| ! | AGTGTAATCAAAACATGTAT+TGG | - | chr8.3:19154060-19154079 | None:intergenic | 25.0% |
| ! | ATGAAGATGTTTATAGGTTA+TGG | + | chr8.3:19155485-19155504 | MS.gene37233:CDS | 25.0% |
| ! | CAAACTATGAAGATGTTTAT+AGG | + | chr8.3:19155479-19155498 | MS.gene37233:CDS | 25.0% |
| ! | GAAGATGATAACATGTAAAA+AGG | + | chr8.3:19154517-19154536 | MS.gene37233:CDS | 25.0% |
| ! | GACATCCAAAAATAAGAATA+TGG | - | chr8.3:19154599-19154618 | None:intergenic | 25.0% |
| ! | TCATTTCTATTTCAATCCAA+AGG | + | chr8.3:19154356-19154375 | MS.gene37233:CDS | 25.0% |
| ! | TGAAGATGTTTATAGGTTAT+GGG | + | chr8.3:19155486-19155505 | MS.gene37233:CDS | 25.0% |
| ! | TGGCATTTAATATATTTGAC+TGG | + | chr8.3:19155224-19155243 | MS.gene37233:CDS | 25.0% |
| ! | TTTACTGACACTATTTGAAT+TGG | - | chr8.3:19153853-19153872 | None:intergenic | 25.0% |
| !! | GAAAATCTATTTTCATCATG+TGG | - | chr8.3:19155154-19155173 | None:intergenic | 25.0% |
| !! | TATATGAGTCTTGTAAATGT+GGG | - | chr8.3:19153947-19153966 | None:intergenic | 25.0% |
| !! | TGTTTTTCCACTTTATTCAA+AGG | + | chr8.3:19154562-19154581 | MS.gene37233:CDS | 25.0% |
| !! | TTATATGAGTCTTGTAAATG+TGG | - | chr8.3:19153948-19153967 | None:intergenic | 25.0% |
| !!! | AATACATGTTTTGATTACAC+TGG | + | chr8.3:19154059-19154078 | MS.gene37233:CDS | 25.0% |
| !!! | GAACTTGATATTTTTCAAAC+AGG | - | chr8.3:19155320-19155339 | None:intergenic | 25.0% |
| !!! | GATCACCATATTCTTATTTT+TGG | + | chr8.3:19154591-19154610 | MS.gene37233:CDS | 25.0% |
| !!! | TTCTTATTTTTGGATGTCTT+TGG | + | chr8.3:19154601-19154620 | MS.gene37233:CDS | 25.0% |
| AATAAACAACCAAATCCAGA+TGG | - | chr8.3:19154758-19154777 | None:intergenic | 30.0% | |
| AATGAAAACTAAGGCGTAAT+CGG | - | chr8.3:19154300-19154319 | None:intergenic | 30.0% | |
| CCAAAAATAAATTCACATCG+TGG | + | chr8.3:19155193-19155212 | MS.gene37233:CDS | 30.0% | |
| CTCAAATGAGTATAGGATAT+TGG | + | chr8.3:19155429-19155448 | MS.gene37233:CDS | 30.0% | |
| CTCATTAGCAATGAAAACTA+AGG | - | chr8.3:19154309-19154328 | None:intergenic | 30.0% | |
| GCAAATGAAGTTGAAAAGTT+AGG | + | chr8.3:19155385-19155404 | MS.gene37233:CDS | 30.0% | |
| TCTCCATTCAATTCCTAAAT+TGG | - | chr8.3:19154489-19154508 | None:intergenic | 30.0% | |
| TGTAAAAAGGAGAAGAGAAA+AGG | + | chr8.3:19154530-19154549 | MS.gene37233:CDS | 30.0% | |
| ! | ATTTTGTGGAAAAAGAGAGA+TGG | + | chr8.3:19155536-19155555 | MS.gene37233:CDS | 30.0% |
| ! | TTCATTTGCTTTAGTTTCCA+AGG | - | chr8.3:19155374-19155393 | None:intergenic | 30.0% |
| ! | TTTTATCAGCAGAATTCACA+TGG | + | chr8.3:19154462-19154481 | MS.gene37233:CDS | 30.0% |
| !! | CCACGATGTGAATTTATTTT+TGG | - | chr8.3:19155196-19155215 | None:intergenic | 30.0% |
| !! | CTTAGTTTTCATTGCTAATG+AGG | + | chr8.3:19154307-19154326 | MS.gene37233:CDS | 30.0% |
| !! | TGAATTGGAAGTGTTATCAT+GGG | - | chr8.3:19153838-19153857 | None:intergenic | 30.0% |
| !! | TTGAATTGGAAGTGTTATCA+TGG | - | chr8.3:19153839-19153858 | None:intergenic | 30.0% |
| !!! | TGTTTTGATTACACTGGTTA+TGG | + | chr8.3:19154065-19154084 | MS.gene37233:CDS | 30.0% |
| AAAGTGGCTCAAATGAGTAT+AGG | + | chr8.3:19155422-19155441 | MS.gene37233:CDS | 35.0% | |
| AGATTGGAAATTGAGTGTAG+AGG | + | chr8.3:19155031-19155050 | MS.gene37233:CDS | 35.0% | |
| AGTGTATGATCATAGCAATG+AGG | + | chr8.3:19154394-19154413 | MS.gene37233:CDS | 35.0% | |
| ATCACCATCATCAGAAATTG+TGG | + | chr8.3:19154979-19154998 | MS.gene37233:CDS | 35.0% | |
| ATCTTTGGTGAAAATCCATC+TGG | + | chr8.3:19154740-19154759 | MS.gene37233:CDS | 35.0% | |
| ATGTCTTTGGCACAAGAAAA+TGG | + | chr8.3:19154614-19154633 | MS.gene37233:CDS | 35.0% | |
| CCAAAAGAAAGAGACACTTT+AGG | + | chr8.3:19154671-19154690 | MS.gene37233:CDS | 35.0% | |
| CCACTTTATTCAAAGGTTAC+TGG | + | chr8.3:19154569-19154588 | MS.gene37233:CDS | 35.0% | |
| CCAGTAACCTTTGAATAAAG+TGG | - | chr8.3:19154572-19154591 | None:intergenic | 35.0% | |
| CCTAATAATCCAGAGTTTGA+CGG | - | chr8.3:19154905-19154924 | None:intergenic | 35.0% | |
| GAATTCACATGGTCCAATTT+AGG | + | chr8.3:19154473-19154492 | MS.gene37233:CDS | 35.0% | |
| GACTAAAGAAGAGCAAAATG+AGG | + | chr8.3:19154955-19154974 | MS.gene37233:CDS | 35.0% | |
| GAGAAGTTGTGGAAAATTGA+GGG | - | chr8.3:19153989-19154008 | None:intergenic | 35.0% | |
| GGAAAACAACCATTGAAACA+GGG | - | chr8.3:19153789-19153808 | None:intergenic | 35.0% | |
| GGAATAATATCTCTCTAAGC+AGG | + | chr8.3:19155293-19155312 | MS.gene37233:CDS | 35.0% | |
| GGAGATGAATGATGAAAGAT+TGG | + | chr8.3:19155015-19155034 | MS.gene37233:CDS | 35.0% | |
| GGTCCAATTTAGGAATTGAA+TGG | + | chr8.3:19154483-19154502 | MS.gene37233:CDS | 35.0% | |
| TGAGAAGTTGTGGAAAATTG+AGG | - | chr8.3:19153990-19154009 | None:intergenic | 35.0% | |
| TGGAAAACAACCATTGAAAC+AGG | - | chr8.3:19153790-19153809 | None:intergenic | 35.0% | |
| TTAGGAAATGCTACAAGCAT+GGG | + | chr8.3:19154812-19154831 | MS.gene37233:CDS | 35.0% | |
| TTGATCTACTTGAGAAGTTG+TGG | - | chr8.3:19154000-19154019 | None:intergenic | 35.0% | |
| TTTAGGAAATGCTACAAGCA+TGG | + | chr8.3:19154811-19154830 | MS.gene37233:CDS | 35.0% | |
| TTTCCACAAAATCAGCATCA+AGG | - | chr8.3:19155528-19155547 | None:intergenic | 35.0% | |
| ! | ATGGTTTGTGAAATGTGTGT+TGG | - | chr8.3:19153916-19153935 | None:intergenic | 35.0% |
| ! | ATTGATAGTTGCAAGAAGCA+AGG | + | chr8.3:19154431-19154450 | MS.gene37233:CDS | 35.0% |
| ! | TGGTTTGTGAAATGTGTGTT+GGG | - | chr8.3:19153915-19153934 | None:intergenic | 35.0% |
| !! | CCTAAAGTGTCTCTTTCTTT+TGG | - | chr8.3:19154674-19154693 | None:intergenic | 35.0% |
| !!! | ATTGGTTTTGACTGCAACAT+TGG | + | chr8.3:19155447-19155466 | MS.gene37233:CDS | 35.0% |
| !!! | ATTTGAGCCACTTTTCTTCT+TGG | - | chr8.3:19155416-19155435 | None:intergenic | 35.0% |
| !!! | TTGGTTTTGACTGCAACATT+GGG | + | chr8.3:19155448-19155467 | MS.gene37233:CDS | 35.0% |
| AGCTCCACAATTTCTGATGA+TGG | - | chr8.3:19154986-19155005 | None:intergenic | 40.0% | |
| CCGTCAAACTCTGGATTATT+AGG | + | chr8.3:19154902-19154921 | MS.gene37233:CDS | 40.0% | |
| CTTTGGCACAAGAAAATGGT+TGG | + | chr8.3:19154618-19154637 | MS.gene37233:CDS | 40.0% | |
| GGGATAAAGACTTGAAGATG+TGG | - | chr8.3:19153895-19153914 | None:intergenic | 40.0% | |
| GGTGAAAATCCATCTGGATT+TGG | + | chr8.3:19154746-19154765 | MS.gene37233:CDS | 40.0% | |
| GTAAATGTGGGAGAGATTCA+TGG | - | chr8.3:19153935-19153954 | None:intergenic | 40.0% | |
| GTGTGACATTGTGCAAATGT+AGG | - | chr8.3:19154854-19154873 | None:intergenic | 40.0% | |
| TCAGAAATTGTGGAGCTACA+AGG | + | chr8.3:19154989-19155008 | MS.gene37233:CDS | 40.0% | |
| TCATCACAACCCTGTTTCAA+TGG | + | chr8.3:19153777-19153796 | MS.gene37233:CDS | 40.0% | |
| TGATAGGAACCAAGAAGAGA+GGG | + | chr8.3:19155342-19155361 | MS.gene37233:CDS | 40.0% | |
| TGATCATAGCAATGAGGCAT+TGG | + | chr8.3:19154400-19154419 | MS.gene37233:CDS | 40.0% | |
| TTGATAGGAACCAAGAAGAG+AGG | + | chr8.3:19155341-19155360 | MS.gene37233:CDS | 40.0% | |
| TTGTGCAGAAGTTAGCTGAT+AGG | + | chr8.3:19155272-19155291 | MS.gene37233:CDS | 40.0% | |
| !! | CTAGTTCTGATTCTTGTCCT+CGG | - | chr8.3:19154229-19154248 | None:intergenic | 40.0% |
| !! | GGTTATGGGCTTTTGTTTCT+AGG | + | chr8.3:19155500-19155519 | MS.gene37233:CDS | 40.0% |
| !!! | GTTCCTTGATGCTGATTTTG+TGG | + | chr8.3:19155522-19155541 | MS.gene37233:CDS | 40.0% |
| AAGACTTGAAGATGTGGCTG+AGG | - | chr8.3:19153889-19153908 | None:intergenic | 45.0% | |
| AAGTCACAGATACTCTACCG+AGG | + | chr8.3:19154209-19154228 | MS.gene37233:CDS | 45.0% | |
| AATTGTGGAGCTACAAGGCA+TGG | + | chr8.3:19154994-19155013 | MS.gene37233:CDS | 45.0% | |
| GAACCAAGAAGAGAGGGAAT+TGG | + | chr8.3:19155348-19155367 | MS.gene37233:CDS | 45.0% | |
| GAGTTCTCAGAGGCAAGAAA+TGG | - | chr8.3:19153816-19153835 | None:intergenic | 45.0% | |
| GATGATGTTCCTCCAGAACA+TGG | - | chr8.3:19153762-19153781 | None:intergenic | 45.0% | |
| GCTAACTTCTGCACAAGTGT+TGG | - | chr8.3:19155268-19155287 | None:intergenic | 45.0% | |
| GGCATGTCCAAGAAGAAAAG+TGG | + | chr8.3:19155406-19155425 | MS.gene37233:CDS | 45.0% | |
| GTCTTAACCATCTCGTTCTC+AGG | - | chr8.3:19154938-19154957 | None:intergenic | 45.0% | |
| GTTATCATGGGAGTTCTCAG+AGG | - | chr8.3:19153826-19153845 | None:intergenic | 45.0% | |
| TCAGAGGCAAGAAATGGAGA+TGG | - | chr8.3:19153810-19153829 | None:intergenic | 45.0% | |
| ! | TGTGAGAAGCTCTGATCCTT+TGG | - | chr8.3:19154375-19154394 | None:intergenic | 45.0% |
| AGGCCAATTCCCTCTCTTCT+TGG | - | chr8.3:19155354-19155373 | None:intergenic | 50.0% | |
| ATGCAACCACCATGTTCTGG+AGG | + | chr8.3:19153750-19153769 | MS.gene37233:CDS | 50.0% | |
| ATTAGACCACGCAGACTCAG+TGG | + | chr8.3:19155054-19155073 | MS.gene37233:CDS | 50.0% | |
| GATGTTCCTCCAGAACATGG+TGG | - | chr8.3:19153759-19153778 | None:intergenic | 50.0% | |
| TTAGACCACGCAGACTCAGT+GGG | + | chr8.3:19155055-19155074 | MS.gene37233:CDS | 50.0% | |
| TTCACATCGTGGACCTACAG+TGG | + | chr8.3:19155204-19155223 | MS.gene37233:CDS | 50.0% | |
| ! | AGAAGAGAGGGAATTGGCCT+TGG | + | chr8.3:19155354-19155373 | MS.gene37233:CDS | 50.0% |
| ! | GACGGTGCACTCTCATTTTG+AGG | - | chr8.3:19154887-19154906 | None:intergenic | 50.0% |
| CAAGTAGCGAGTAGACGACG+AGG | - | chr8.3:19154141-19154160 | None:intergenic | 55.0% | |
| CGCTTCTCCTGAGAACGAGA+TGG | + | chr8.3:19154928-19154947 | MS.gene37233:CDS | 55.0% | |
| TTAGTCCCACTGAGTCTGCG+TGG | - | chr8.3:19155063-19155082 | None:intergenic | 55.0% | |
| ! | GAGAGTGCACCGTCAAACTC+TGG | + | chr8.3:19154893-19154912 | MS.gene37233:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 19153750 | 19155597 | 19153750 | ID=MS.gene37233 |
| chr8.3 | mRNA | 19153750 | 19155597 | 19153750 | ID=MS.gene37233.t1;Parent=MS.gene37233 |
| chr8.3 | exon | 19153750 | 19155597 | 19153750 | ID=MS.gene37233.t1.exon1;Parent=MS.gene37233.t1 |
| chr8.3 | CDS | 19153750 | 19155597 | 19153750 | ID=cds.MS.gene37233.t1;Parent=MS.gene37233.t1 |
| Gene Sequence |
| Protein sequence |
