AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene45578


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45578.t1 MTR_3g037570 95.652 138 6 0 1 138 1 138 6.89e-94 268
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45578.t1 AT1G26550 87.603 121 15 0 18 138 16 136 1.08e-68 204

Find 0 sgRNAs with CRISPR-Local

Find 112 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTATTATTTTCTTTACTT+TGG + 23426:65616-65635 MS.gene45578:intron 10.0%
!! ATATATAATAACAACTAGAA+GGG - 23426:66381-66400 None:intergenic 15.0%
!!! AAATGCTTTTCAAAAATAAA+CGG + 23426:65397-65416 MS.gene45578:intron 15.0%
!!! ATATTTTGATATCAACAAAT+CGG - 23426:66006-66025 None:intergenic 15.0%
!! CATATATAATAACAACTAGA+AGG - 23426:66382-66401 None:intergenic 20.0%
!! TTAAATAGAAAGCCAAATAA+GGG - 23426:65820-65839 None:intergenic 20.0%
!! TTTAAATAGAAAGCCAAATA+AGG - 23426:65821-65840 None:intergenic 20.0%
!!! AATGCTTTTCAAAAATAAAC+GGG + 23426:65398-65417 MS.gene45578:intron 20.0%
!!! ATTTGGCTTTCTATTTAAAA+TGG + 23426:65822-65841 MS.gene45578:intron 20.0%
!!! TTGCAAATAGATTGAAATTT+TGG + 23426:65300-65319 MS.gene45578:intron 20.0%
!!! TTGTTTTCATTGTTTTTGAA+AGG + 23426:65336-65355 MS.gene45578:intron 20.0%
!!! TTTGGCTTTCTATTTAAAAT+GGG + 23426:65823-65842 MS.gene45578:intron 20.0%
! AAACTGTAAAATGAACATAG+TGG - 23426:65280-65299 None:intergenic 25.0%
! AACTGTAAAATGAACATAGT+GGG - 23426:65279-65298 None:intergenic 25.0%
! CGTAGAATTACATTCATTTA+TGG + 23426:65464-65483 MS.gene45578:intron 25.0%
! GAAACTTATTATGTGGTAAA+TGG + 23426:65851-65870 MS.gene45578:intron 25.0%
! GTTGAAGTGAAAATAAAAAC+AGG - 23426:65222-65241 None:intergenic 25.0%
! TATACACTAGGATAAAACAT+AGG + 23426:65643-65662 MS.gene45578:intron 25.0%
!! ACTGCTTGGAAATTTTTAAA+AGG + 23426:65714-65733 MS.gene45578:intron 25.0%
!! ATGCTTTTCAAAAATAAACG+GGG + 23426:65399-65418 MS.gene45578:intron 25.0%
!! TTGGCTTTCTATTTAAAATG+GGG + 23426:65824-65843 MS.gene45578:intron 25.0%
!!! CTGCTTGGAAATTTTTAAAA+GGG + 23426:65715-65734 MS.gene45578:intron 25.0%
AAAAAGAGACTATAACTGCT+TGG + 23426:65700-65719 MS.gene45578:intron 30.0%
ACTGTAAAATGAACATAGTG+GGG - 23426:65278-65297 None:intergenic 30.0%
CATATCTTATGTGAGAAACA+AGG + 23426:65508-65527 MS.gene45578:CDS 30.0%
GTATACACTAAACCCTTATT+TGG + 23426:65805-65824 MS.gene45578:intron 30.0%
! ACTGACATGCATATGTATTT+TGG - 23426:66099-66118 None:intergenic 30.0%
! TACTTTGGAAGTTATACACT+AGG + 23426:65631-65650 MS.gene45578:intron 30.0%
!! ACATGCATATGTATTTTGGA+GGG - 23426:66095-66114 None:intergenic 30.0%
ATTGAAGTAAATGAACCCTG+AGG - 23426:65194-65213 None:intergenic 35.0%
GGAAGGGGATAAAATAACTA+TGG - 23426:65884-65903 None:intergenic 35.0%
TGAAAGAAATTTAGCGTGTC+TGG - 23426:65434-65453 None:intergenic 35.0%
!! ATGCATATGTATTTTGGAGG+GGG - 23426:66093-66112 None:intergenic 35.0%
!! CATGCATATGTATTTTGGAG+GGG - 23426:66094-66113 None:intergenic 35.0%
!! GACATGCATATGTATTTTGG+AGG - 23426:66096-66115 None:intergenic 35.0%
!! GAGCAATACGTTGATTTGAA+AGG - 23426:66353-66372 None:intergenic 35.0%
AAGTGATGAGAATGCTTCCA+AGG + 23426:65084-65103 MS.gene45578:CDS 40.0%
AGTGATGAGAATGCTTCCAA+GGG + 23426:65085-65104 MS.gene45578:CDS 40.0%
GCATTGAATCCATTTCTGAG+CGG - 23426:65905-65924 None:intergenic 40.0%
GGGCTGAGAAACTTATTATG+TGG + 23426:65844-65863 MS.gene45578:intron 40.0%
GGTACTTGCACTTATGTCAA+AGG + 23426:65142-65161 MS.gene45578:CDS 40.0%
GTCAGTAAATACCCGTCAAT+GGG - 23426:66053-66072 None:intergenic 40.0%
TATACCTTTGCAAACTCTGC+TGG - 23426:65598-65617 None:intergenic 40.0%
TGAAGGCAACTTCCTGAAAT+GGG - 23426:66304-66323 None:intergenic 40.0%
TTGAAGGCAACTTCCTGAAA+TGG - 23426:66305-66324 None:intergenic 40.0%
! GAACATAGTGGGGAAACTAT+TGG - 23426:65268-65287 None:intergenic 40.0%
!! AAGATGGTTGGCTTGACAAT+GGG + 23426:65560-65579 MS.gene45578:CDS 40.0%
!! ATGCATGTCAGTTTTGTCGA+AGG + 23426:66107-66126 MS.gene45578:intron 40.0%
!! TGCATATGTATTTTGGAGGG+GGG - 23426:66092-66111 None:intergenic 40.0%
AACTCCAACAGGTGTGTTGA+AGG - 23426:66321-66340 None:intergenic 45.0%
AATCCATTTCTGAGCGGAAG+GGG - 23426:65899-65918 None:intergenic 45.0%
ACAAGAAGCTGCAAGATGGT+TGG + 23426:65548-65567 MS.gene45578:CDS 45.0%
ACAGCTGAATTCAAGACTGC+TGG - 23426:65939-65958 None:intergenic 45.0%
ACCATCTTGCAGCTTCTTGT+AGG - 23426:65548-65567 None:intergenic 45.0%
CCAAGGGAAAAGGAAAAGGT+GGG + 23426:65101-65120 MS.gene45578:CDS 45.0%
CTGCATACAATGCCCATTGA+CGG + 23426:66038-66057 MS.gene45578:intron 45.0%
GAATCCATTTCTGAGCGGAA+GGG - 23426:65900-65919 None:intergenic 45.0%
GAATGCCCATCAGGAAAGAA+GGG + 23426:66239-66258 MS.gene45578:CDS 45.0%
GAGAATGCTTCCAAGGGAAA+AGG + 23426:65091-65110 MS.gene45578:CDS 45.0%
GAGTATTCTGAATGCCCATC+AGG + 23426:66230-66249 MS.gene45578:CDS 45.0%
GCAAAGGAGTCAAAGGGTAA+GGG + 23426:65043-65062 MS.gene45578:CDS 45.0%
GCAAAGGCAAAGGAGTCAAA+GGG + 23426:65037-65056 MS.gene45578:CDS 45.0%
GCAGTCTTGAATTCAGCTGT+AGG + 23426:65939-65958 MS.gene45578:intron 45.0%
GCTTCCAAGGGAAAAGGAAA+AGG + 23426:65097-65116 MS.gene45578:CDS 45.0%
GGTCAGTAAATACCCGTCAA+TGG - 23426:66054-66073 None:intergenic 45.0%
TCCAAGGGAAAAGGAAAAGG+TGG + 23426:65100-65119 MS.gene45578:CDS 45.0%
TGAATCCATTTCTGAGCGGA+AGG - 23426:65901-65920 None:intergenic 45.0%
TGAATGCCCATCAGGAAAGA+AGG + 23426:66238-66257 MS.gene45578:CDS 45.0%
TGCATACAATGCCCATTGAC+GGG + 23426:66039-66058 MS.gene45578:intron 45.0%
! TTGGCTTGACAATGGGGATA+AGG + 23426:65567-65586 MS.gene45578:CDS 45.0%
!! AGATGGTTGGCTTGACAATG+GGG + 23426:65561-65580 MS.gene45578:CDS 45.0%
!! CAAGATGGTTGGCTTGACAA+TGG + 23426:65559-65578 MS.gene45578:CDS 45.0%
!! CGTTGATTTGAAAGGAGCAC+TGG - 23426:66345-66364 None:intergenic 45.0%
AAAGGTGGGAAAGCTGCTGA+TGG + 23426:65115-65134 MS.gene45578:CDS 50.0%
AAGGTGGGAAAGCTGCTGAT+GGG + 23426:65116-65135 MS.gene45578:CDS 50.0%
ACCTTTGCAAACTCTGCTGG+AGG - 23426:65595-65614 None:intergenic 50.0%
AGACCTTGGATGGTTTCCAC+GGG + 23426:66265-66284 MS.gene45578:CDS 50.0%
ATACTCTTGAGCTACCTGCG+AGG - 23426:66216-66235 None:intergenic 50.0%
ATGGTTTCCACGGGGAAAGA+TGG + 23426:66274-66293 MS.gene45578:CDS 50.0%
CGCAAAGGCAAAGGAGTCAA+AGG + 23426:65036-65055 MS.gene45578:CDS 50.0%
CTCCACCCTTCTTTCCTGAT+GGG - 23426:66247-66266 None:intergenic 50.0%
GACGGGTATTTACTGACCAC+AGG + 23426:66056-66075 MS.gene45578:intron 50.0%
GAGACCTTGGATGGTTTCCA+CGG + 23426:66264-66283 MS.gene45578:CDS 50.0%
GCCTACAAGAAGCTGCAAGA+TGG + 23426:65544-65563 MS.gene45578:CDS 50.0%
GGCAAAGGAGTCAAAGGGTA+AGG + 23426:65042-65061 MS.gene45578:CDS 50.0%
GGGAAAAGACGCAAAGGCAA+AGG + 23426:65027-65046 MS.gene45578:CDS 50.0%
GTCAAAGGGTAAGGGAAAGC+AGG + 23426:65051-65070 MS.gene45578:CDS 50.0%
GTTGCCTTCAACACACCTGT+TGG + 23426:66314-66333 MS.gene45578:CDS 50.0%
TAATATTTCGCCGACCTCGC+AGG + 23426:66199-66218 MS.gene45578:intron 50.0%
TATCCCCTTCCGCTCAGAAA+TGG + 23426:65893-65912 MS.gene45578:intron 50.0%
TCCTCCAGCAGAGTTTGCAA+AGG + 23426:65591-65610 MS.gene45578:CDS 50.0%
TCTCCACCCTTCTTTCCTGA+TGG - 23426:66248-66267 None:intergenic 50.0%
! AAAGATGGCTGGCCCATTTC+AGG + 23426:66289-66308 MS.gene45578:CDS 50.0%
! AACCCTGAGGTGCTTGTGAA+TGG - 23426:65181-65200 None:intergenic 50.0%
! ACCCTGAGGTGCTTGTGAAT+GGG - 23426:65180-65199 None:intergenic 50.0%
! CCCACCTTTTCCTTTTCCCT+TGG - 23426:65104-65123 None:intergenic 50.0%
!! TGTATTTTGGAGGGGGGATC+AGG - 23426:66086-66105 None:intergenic 50.0%
AAGGGAAAGCAGGCTGCAAG+TGG + 23426:65061-65080 MS.gene45578:CDS 55.0%
AGAAGGGTGGAGACCTTGGA+TGG + 23426:66255-66274 MS.gene45578:CDS 55.0%
CCCCATTCACAAGCACCTCA+GGG + 23426:65176-65195 MS.gene45578:intron 55.0%
GACCTTGGATGGTTTCCACG+GGG + 23426:66266-66285 MS.gene45578:CDS 55.0%
GGAAAGAAGGGTGGAGACCT+TGG + 23426:66251-66270 MS.gene45578:CDS 55.0%
TCCCCATTCACAAGCACCTC+AGG + 23426:65175-65194 MS.gene45578:intron 55.0%
TCTTGAGCTACCTGCGAGGT+CGG - 23426:66212-66231 None:intergenic 55.0%
TGCCCATCAGGAAAGAAGGG+TGG + 23426:66242-66261 MS.gene45578:CDS 55.0%
TTCCCCGTGGAAACCATCCA+AGG - 23426:66271-66290 None:intergenic 55.0%
TTTCCACGGGGAAAGATGGC+TGG + 23426:66278-66297 MS.gene45578:CDS 55.0%
! CCCTGAGGTGCTTGTGAATG+GGG - 23426:65179-65198 None:intergenic 55.0%
!! GGAGCACTGGTAACTCCAAC+AGG - 23426:66332-66351 None:intergenic 55.0%
GGGGGGATCAGGATTTCCTG+TGG - 23426:66075-66094 None:intergenic 60.0%
!! GGGAAAGCTGCTGATGGGCT+TGG + 23426:65121-65140 MS.gene45578:CDS 60.0%
GGGCCAGCCATCTTTCCCCG+TGG - 23426:66284-66303 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
23426 gene 65025 66415 65025 ID=MS.gene45578
23426 mRNA 65025 66415 65025 ID=MS.gene45578.t1;Parent=MS.gene45578
23426 exon 65025 65163 65025 ID=MS.gene45578.t1.exon1;Parent=MS.gene45578.t1
23426 CDS 65025 65163 65025 ID=cds.MS.gene45578.t1;Parent=MS.gene45578.t1
23426 exon 65503 65612 65503 ID=MS.gene45578.t1.exon2;Parent=MS.gene45578.t1
23426 CDS 65503 65612 65503 ID=cds.MS.gene45578.t1;Parent=MS.gene45578.t1
23426 exon 66221 66415 66221 ID=MS.gene45578.t1.exon3;Parent=MS.gene45578.t1
23426 CDS 66221 66415 66221 ID=cds.MS.gene45578.t1;Parent=MS.gene45578.t1
Gene Sequence

>MS.gene45578

ATGGGAAAAGACGCAAAGGCAAAGGAGTCAAAGGGTAAGGGAAAGCAGGCTGCAAGTGGAAGTGATGAGAATGCTTCCAAGGGAAAAGGAAAAGGTGGGAAAGCTGCTGATGGGCTTGGTACTTGCACTTATGTCAAAGGTACAAAATTTTCCCCATTCACAAGCACCTCAGGGTTCATTTACTTCAATTGTTTCCTGTTTTTATTTTCACTTCAACTATAAACATAAGAGAATATTGATCCAATAGTTTCCCCACTATGTTCATTTTACAGTTTTTGCAAATAGATTGAAATTTTGGAAATGTCACAACATTGTTTTCATTGTTTTTGAAAGGTTCATACTTGAACATAAATTTTAGAAAATGAAAACTGAAAATGCTTTTCAAAAATAAACGGGGCTAAAATAACCAGACACGCTAAATTTCTTTCATTATTTTTCTCGTAGAATTACATTCATTTATGGCTTTTACTTTGCTCAGCAAGACATATCTTATGTGAGAAACAAGGTAAGATTAATGAAGCCTACAAGAAGCTGCAAGATGGTTGGCTTGACAATGGGGATAAGGTTCCTCCAGCAGAGTTTGCAAAGGTATATTATTATTTTCTTTACTTTGGAAGTTATACACTAGGATAAAACATAGGCAGTGCTATACATTTGTTACTACTGTTTTCTAGTAAAAAGAGACTATAACTGCTTGGAAATTTTTAAAAGGGTGCTAAAAATCTCTTGACAAAAAAAATAAAAATAAAAGAACAGTTAGAAATGTATCTAAGTTTTGTCGTATACACTAAACCCTTATTTGGCTTTCTATTTAAAATGGGGCTGAGAAACTTATTATGTGGTAAATGGCTAAATGCCATAGTTATTTTATCCCCTTCCGCTCAGAAATGGATTCAATGCAGTTGATATTTCCAGCAGTCTTGAATTCAGCTGTAGGATGAAAAGATCAGACGACTCAGATTTTGAATTGAATTAAATCCGATTTGTTGATATCAAAATATGAGTTGTCTGATCTGCATACAATGCCCATTGACGGGTATTTACTGACCACAGGAAATCCTGATCCCCCCTCCAAAATACATATGCATGTCAGTTTTGTCGAAGGAATGAGAAATAAGAATCAAAATTTGTCTTGAGATAGAAATATTCTGTCTTTTCAATTGTTTATCACTGATAATATTTCGCCGACCTCGCAGGTAGCTCAAGAGTATTCTGAATGCCCATCAGGAAAGAAGGGTGGAGACCTTGGATGGTTTCCACGGGGAAAGATGGCTGGCCCATTTCAGGAAGTTGCCTTCAACACACCTGTTGGAGTTACCAGTGCTCCTTTCAAATCAACGTATTGCTCTCGCACCCTTCTAGTTGTTATTATATATGCATTATTGCTCTGA

Protein sequence

>MS.gene45578.t1

MGKDAKAKESKGKGKQAASGSDENASKGKGKGGKAADGLGTCTYVKARHILCEKQGKINEAYKKLQDGWLDNGDKVPPAEFAKVAQEYSECPSGKKGGDLGWFPRGKMAGPFQEVAFNTPVGVTSAPFKSTYCSRTLLVVIIYALLL