AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene49065


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49065.t1 MTR_1g100753 95.631 206 9 0 1 206 1 206 1.74e-136 380
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49065.t1 AT5G52190 72.637 201 50 2 11 206 8 208 1.26e-106 305

Find 69 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGAAAATATGGAGGATATT+TGG 0.228871 1.3:-69689447 None:intergenic
GAGATGGTTGTGTATAAGTT+AGG 0.266389 1.3:+69689925 MS.gene49065:CDS
TTCTATTTCTCAAGTAGATA+TGG 0.316510 1.3:+69689399 MS.gene49065:CDS
TGACGAGATCGAACGGCTTC+AGG 0.341605 1.3:-69689967 None:intergenic
CTGACTGCTCAACCAGAGAT+AGG 0.342066 1.3:+69689763 MS.gene49065:CDS
TGGTCATGTCGAAGACTAGA+TGG 0.365756 1.3:-69689627 None:intergenic
AGAGGAGATGGTGGAGAGTT+CGG 0.366138 1.3:-69689512 None:intergenic
AGATGGTTGTGTATAAGTTA+GGG 0.370004 1.3:+69689926 MS.gene49065:CDS
GATGGGCTGAGAGGCCTAGA+TGG 0.374597 1.3:-69689609 None:intergenic
AGTAAGAGGTCGAGAGGAGG+TGG 0.378790 1.3:-69689487 None:intergenic
ATCAAGACCATTGCTTCCTA+TGG 0.384419 1.3:+69689867 MS.gene49065:CDS
TCGATCTCGTCATACTAATC+TGG 0.390912 1.3:+69689978 MS.gene49065:CDS
TTACGTGCCTGCAAAGACTA+TGG 0.402934 1.3:+69689816 MS.gene49065:CDS
AGGTCGAGAGGAGGTGGGTG+TGG 0.409570 1.3:-69689481 None:intergenic
AGGAGATCATTGGTGGTGAT+TGG 0.410627 1.3:-69689655 None:intergenic
TCAAGTAGATATGGCTTCAA+TGG 0.418159 1.3:+69689408 MS.gene49065:CDS
CTCTACGGTGTTGGCCGCGA+AGG 0.422166 1.3:+69689553 MS.gene49065:CDS
TCATTCGCCATAGTCTTTGC+AGG 0.428098 1.3:-69689823 None:intergenic
CTCATCGCCTCAGCCGGTCC+AGG 0.437578 1.3:+69689676 MS.gene49065:CDS
TCGACAGTGGAGAAGCCGCC+TGG 0.438096 1.3:-69689694 None:intergenic
GGAGGTGGGTGTGGATGAGT+GGG 0.445781 1.3:-69689472 None:intergenic
AGGAGGTGGGTGTGGATGAG+TGG 0.447308 1.3:-69689473 None:intergenic
ATGAGTGGGAGTTGAAAATA+TGG 0.453698 1.3:-69689458 None:intergenic
TTCACACAGGACCCTATCTC+TGG 0.470565 1.3:-69689775 None:intergenic
ATGGGCTAAGCGCATGCATA+AGG 0.471381 1.3:-69689590 None:intergenic
ACTGTTTGTGTTGTTTGAGA+TGG 0.478342 1.3:+69689909 MS.gene49065:CDS
AGTGGAGAAGCCGCCTGGAC+CGG 0.488941 1.3:-69689689 None:intergenic
AAAGACTATGGCGAATGATA+AGG 0.495854 1.3:+69689828 MS.gene49065:CDS
GATCTCCTCATCGCCTCAGC+CGG 0.497810 1.3:+69689670 MS.gene49065:CDS
AGATCATTGGTGGTGATTGG+AGG 0.498257 1.3:-69689652 None:intergenic
ATGGCGAATGATAAGGATGA+AGG 0.506270 1.3:+69689835 MS.gene49065:CDS
GAAGCCGCCTGGACCGGCTG+AGG 0.507477 1.3:-69689683 None:intergenic
TCAAGACCATTGCTTCCTAT+GGG 0.513407 1.3:+69689868 MS.gene49065:CDS
CACTGTCGATGCTATATGCT+CGG 0.515036 1.3:+69689708 MS.gene49065:CDS
CTTTGAGCATTAAGCCTTCG+CGG 0.520257 1.3:-69689567 None:intergenic
AAGAAGCACTCTTCCACCGT+TGG 0.538774 1.3:-69689740 None:intergenic
TCCACCATCTCCTCTCAAAA+CGG 0.554570 1.3:+69689520 MS.gene49065:CDS
TGAGGCGATGAGGAGATCAT+TGG 0.554801 1.3:-69689665 None:intergenic
TGACTGCTCAACCAGAGATA+GGG 0.560185 1.3:+69689764 MS.gene49065:CDS
GTAAGAGGTCGAGAGGAGGT+GGG 0.564585 1.3:-69689486 None:intergenic
TGGCGAATGATAAGGATGAA+GGG 0.568337 1.3:+69689836 MS.gene49065:CDS
CTGTGTGAAGCATGCTAGTG+TGG 0.570857 1.3:+69689789 MS.gene49065:CDS
TGGAGAGTTCGGTTAGTAAG+AGG 0.579288 1.3:-69689501 None:intergenic
ATGGGCTGAGAGGCCTAGAT+GGG 0.584829 1.3:-69689608 None:intergenic
AGTGGGAGTTGAAAATATGG+AGG 0.589032 1.3:-69689455 None:intergenic
GGCGAATGATAAGGATGAAG+GGG 0.593461 1.3:+69689837 MS.gene49065:CDS
GGTCATGTCGAAGACTAGAT+GGG 0.596297 1.3:-69689626 None:intergenic
CCTATGGGGAGTGTGTATGA+AGG 0.597763 1.3:+69689883 MS.gene49065:CDS
GATGGTTGTGTATAAGTTAG+GGG 0.603756 1.3:+69689927 MS.gene49065:CDS
TGCTCGGTAGCACGATCCAA+CGG 0.606651 1.3:+69689724 MS.gene49065:CDS
CCTTCATACACACTCCCCAT+AGG 0.612186 1.3:-69689883 None:intergenic
ATTAGTATGACGAGATCGAA+CGG 0.612587 1.3:-69689974 None:intergenic
CTGGACCGGCTGAGGCGATG+AGG 0.614008 1.3:-69689675 None:intergenic
ACACTCCCCATAGGAAGCAA+TGG 0.614848 1.3:-69689874 None:intergenic
TCGGTTAGTAAGAGGTCGAG+AGG 0.619034 1.3:-69689493 None:intergenic
GGCGATGAGGAGATCATTGG+TGG 0.627157 1.3:-69689662 None:intergenic
ATTGGTGGTGATTGGAGGAG+TGG 0.628718 1.3:-69689647 None:intergenic
TGTGAAGCATGCTAGTGTGG+TGG 0.631703 1.3:+69689792 MS.gene49065:CDS
TGCATGCGCTTAGCCCATCT+AGG 0.636017 1.3:+69689595 MS.gene49065:CDS
CACACTAGCATGCTTCACAC+AGG 0.655929 1.3:-69689788 None:intergenic
TATTTGGTTGCAGATTTGTG+TGG 0.656311 1.3:-69689431 None:intergenic
GTTAGTAAGAGGTCGAGAGG+AGG 0.664837 1.3:-69689490 None:intergenic
CGAAGACTAGATGGGCTGAG+AGG 0.670570 1.3:-69689618 None:intergenic
CTTCGCGGCCAACACCGTAG+AGG 0.673681 1.3:-69689552 None:intergenic
CGAGCATATAGCATCGACAG+TGG 0.676439 1.3:-69689707 None:intergenic
CAAGACCATTGCTTCCTATG+GGG 0.677128 1.3:+69689869 MS.gene49065:CDS
CAACACCGTAGAGGAAAACG+CGG 0.688293 1.3:-69689543 None:intergenic
ATCGCCTCAGCCGGTCCAGG+CGG 0.693754 1.3:+69689679 MS.gene49065:CDS
TCGGTAGCACGATCCAACGG+TGG 0.767760 1.3:+69689727 MS.gene49065:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TTGAAAATATGGAGGATATT+TGG - chr1.3:69689450-69689469 None:intergenic 25.0%
!! TTCTATTTCTCAAGTAGATA+TGG + chr1.3:69689399-69689418 MS.gene49065:CDS 25.0%
AGATGGTTGTGTATAAGTTA+GGG + chr1.3:69689926-69689945 MS.gene49065:CDS 30.0%
! TATAAGTTAGGGGAGATTTT+AGG + chr1.3:69689937-69689956 MS.gene49065:CDS 30.0%
AAAGACTATGGCGAATGATA+AGG + chr1.3:69689828-69689847 MS.gene49065:CDS 35.0%
ATGAGTGGGAGTTGAAAATA+TGG - chr1.3:69689461-69689480 None:intergenic 35.0%
ATTAGTATGACGAGATCGAA+CGG - chr1.3:69689977-69689996 None:intergenic 35.0%
GAGATGGTTGTGTATAAGTT+AGG + chr1.3:69689925-69689944 MS.gene49065:CDS 35.0%
GATGGTTGTGTATAAGTTAG+GGG + chr1.3:69689927-69689946 MS.gene49065:CDS 35.0%
TATTTGGTTGCAGATTTGTG+TGG - chr1.3:69689434-69689453 None:intergenic 35.0%
TCAAGTAGATATGGCTTCAA+TGG + chr1.3:69689408-69689427 MS.gene49065:CDS 35.0%
!! ACTGTTTGTGTTGTTTGAGA+TGG + chr1.3:69689909-69689928 MS.gene49065:CDS 35.0%
AGTGGGAGTTGAAAATATGG+AGG - chr1.3:69689458-69689477 None:intergenic 40.0%
ATCAAGACCATTGCTTCCTA+TGG + chr1.3:69689867-69689886 MS.gene49065:CDS 40.0%
ATGGCGAATGATAAGGATGA+AGG + chr1.3:69689835-69689854 MS.gene49065:CDS 40.0%
TCAAGACCATTGCTTCCTAT+GGG + chr1.3:69689868-69689887 MS.gene49065:CDS 40.0%
TCGATCTCGTCATACTAATC+TGG + chr1.3:69689978-69689997 MS.gene49065:CDS 40.0%
TGGCGAATGATAAGGATGAA+GGG + chr1.3:69689836-69689855 MS.gene49065:CDS 40.0%
CAAGACCATTGCTTCCTATG+GGG + chr1.3:69689869-69689888 MS.gene49065:CDS 45.0%
CACTGTCGATGCTATATGCT+CGG + chr1.3:69689708-69689727 MS.gene49065:CDS 45.0%
CTTTGAGCATTAAGCCTTCG+CGG - chr1.3:69689570-69689589 None:intergenic 45.0%
GGCGAATGATAAGGATGAAG+GGG + chr1.3:69689837-69689856 MS.gene49065:CDS 45.0%
GGTCATGTCGAAGACTAGAT+GGG - chr1.3:69689629-69689648 None:intergenic 45.0%
TCATTCGCCATAGTCTTTGC+AGG - chr1.3:69689826-69689845 None:intergenic 45.0%
TCCACCATCTCCTCTCAAAA+CGG + chr1.3:69689520-69689539 MS.gene49065:CDS 45.0%
TGACTGCTCAACCAGAGATA+GGG + chr1.3:69689764-69689783 MS.gene49065:CDS 45.0%
TGGAGAGTTCGGTTAGTAAG+AGG - chr1.3:69689504-69689523 None:intergenic 45.0%
TGGTCATGTCGAAGACTAGA+TGG - chr1.3:69689630-69689649 None:intergenic 45.0%
TTACGTGCCTGCAAAGACTA+TGG + chr1.3:69689816-69689835 MS.gene49065:CDS 45.0%
!! AGATCATTGGTGGTGATTGG+AGG - chr1.3:69689655-69689674 None:intergenic 45.0%
!! AGGAGATCATTGGTGGTGAT+TGG - chr1.3:69689658-69689677 None:intergenic 45.0%
ACACTCCCCATAGGAAGCAA+TGG - chr1.3:69689877-69689896 None:intergenic 50.0%
CAACACCGTAGAGGAAAACG+CGG - chr1.3:69689546-69689565 None:intergenic 50.0%
CACACTAGCATGCTTCACAC+AGG - chr1.3:69689791-69689810 None:intergenic 50.0%
CCTTCATACACACTCCCCAT+AGG - chr1.3:69689886-69689905 None:intergenic 50.0%
CTGACTGCTCAACCAGAGAT+AGG + chr1.3:69689763-69689782 MS.gene49065:CDS 50.0%
CTGTGTGAAGCATGCTAGTG+TGG + chr1.3:69689789-69689808 MS.gene49065:CDS 50.0%
GTTAGTAAGAGGTCGAGAGG+AGG - chr1.3:69689493-69689512 None:intergenic 50.0%
TCGGTTAGTAAGAGGTCGAG+AGG - chr1.3:69689496-69689515 None:intergenic 50.0%
TGAGGCGATGAGGAGATCAT+TGG - chr1.3:69689668-69689687 None:intergenic 50.0%
TGTGAAGCATGCTAGTGTGG+TGG + chr1.3:69689792-69689811 MS.gene49065:CDS 50.0%
TTCACACAGGACCCTATCTC+TGG - chr1.3:69689778-69689797 None:intergenic 50.0%
! ATGGGCTAAGCGCATGCATA+AGG - chr1.3:69689593-69689612 None:intergenic 50.0%
! CCTATGGGGAGTGTGTATGA+AGG + chr1.3:69689883-69689902 MS.gene49065:CDS 50.0%
!! AAGAAGCACTCTTCCACCGT+TGG - chr1.3:69689743-69689762 None:intergenic 50.0%
!! AGAGGAGATGGTGGAGAGTT+CGG - chr1.3:69689515-69689534 None:intergenic 50.0%
!! ATTGGTGGTGATTGGAGGAG+TGG - chr1.3:69689650-69689669 None:intergenic 50.0%
!! CGAGCATATAGCATCGACAG+TGG - chr1.3:69689710-69689729 None:intergenic 50.0%
AGTAAGAGGTCGAGAGGAGG+TGG - chr1.3:69689490-69689509 None:intergenic 55.0%
GGCGATGAGGAGATCATTGG+TGG - chr1.3:69689665-69689684 None:intergenic 55.0%
GTAAGAGGTCGAGAGGAGGT+GGG - chr1.3:69689489-69689508 None:intergenic 55.0%
TGACGAGATCGAACGGCTTC+AGG - chr1.3:69689970-69689989 None:intergenic 55.0%
TGCATGCGCTTAGCCCATCT+AGG + chr1.3:69689595-69689614 MS.gene49065:CDS 55.0%
TGCTCGGTAGCACGATCCAA+CGG + chr1.3:69689724-69689743 MS.gene49065:CDS 55.0%
! AACGGCCGCGTTTTCCTCTA+CGG + chr1.3:69689538-69689557 MS.gene49065:CDS 55.0%
! ATGGGCTGAGAGGCCTAGAT+GGG - chr1.3:69689611-69689630 None:intergenic 55.0%
! CGCGTTTTCCTCTACGGTGT+TGG + chr1.3:69689544-69689563 MS.gene49065:CDS 55.0%
!! CGAAGACTAGATGGGCTGAG+AGG - chr1.3:69689621-69689640 None:intergenic 55.0%
!! GAAAACGCGGCCGTTTTGAG+AGG - chr1.3:69689533-69689552 None:intergenic 55.0%
!!! GCCGTTTTGAGAGGAGATGG+TGG - chr1.3:69689524-69689543 None:intergenic 55.0%
AGGAGGTGGGTGTGGATGAG+TGG - chr1.3:69689476-69689495 None:intergenic 60.0%
GATCTCCTCATCGCCTCAGC+CGG + chr1.3:69689670-69689689 MS.gene49065:CDS 60.0%
GGAGGTGGGTGTGGATGAGT+GGG - chr1.3:69689475-69689494 None:intergenic 60.0%
TCGGTAGCACGATCCAACGG+TGG + chr1.3:69689727-69689746 MS.gene49065:CDS 60.0%
!! GATGGGCTGAGAGGCCTAGA+TGG - chr1.3:69689612-69689631 None:intergenic 60.0%
!!! GCGGCCGTTTTGAGAGGAGA+TGG - chr1.3:69689527-69689546 None:intergenic 60.0%
AGGTCGAGAGGAGGTGGGTG+TGG - chr1.3:69689484-69689503 None:intergenic 65.0%
AGTGGAGAAGCCGCCTGGAC+CGG - chr1.3:69689692-69689711 None:intergenic 65.0%
CTTCGCGGCCAACACCGTAG+AGG - chr1.3:69689555-69689574 None:intergenic 65.0%
TCGACAGTGGAGAAGCCGCC+TGG - chr1.3:69689697-69689716 None:intergenic 65.0%
!! CTCTACGGTGTTGGCCGCGA+AGG + chr1.3:69689553-69689572 MS.gene49065:CDS 65.0%
ATCGCCTCAGCCGGTCCAGG+CGG + chr1.3:69689679-69689698 MS.gene49065:CDS 70.0%
CTCATCGCCTCAGCCGGTCC+AGG + chr1.3:69689676-69689695 MS.gene49065:CDS 70.0%
CTGGACCGGCTGAGGCGATG+AGG - chr1.3:69689678-69689697 None:intergenic 70.0%
GAAGCCGCCTGGACCGGCTG+AGG - chr1.3:69689686-69689705 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 69689385 69690005 69689385 ID=MS.gene49065
chr1.3 mRNA 69689385 69690005 69689385 ID=MS.gene49065.t1;Parent=MS.gene49065
chr1.3 exon 69689385 69690005 69689385 ID=MS.gene49065.t1.exon1;Parent=MS.gene49065.t1
chr1.3 CDS 69689385 69690005 69689385 ID=cds.MS.gene49065.t1;Parent=MS.gene49065.t1
Gene Sequence

>MS.gene49065

ATGGCTTCTTCTAGTTCTATTTCTCAAGTAGATATGGCTTCAATGGCCACACAAATCTGCAACCAAATATCCTCCATATTTTCAACTCCCACTCATCCACACCCACCTCCTCTCGACCTCTTACTAACCGAACTCTCCACCATCTCCTCTCAAAACGGCCGCGTTTTCCTCTACGGTGTTGGCCGCGAAGGCTTAATGCTCAAAGCCTTATGCATGCGCTTAGCCCATCTAGGCCTCTCAGCCCATCTAGTCTTCGACATGACCACTCCTCCAATCACCACCAATGATCTCCTCATCGCCTCAGCCGGTCCAGGCGGCTTCTCCACTGTCGATGCTATATGCTCGGTAGCACGATCCAACGGTGGAAGAGTGCTTCTTCTGACTGCTCAACCAGAGATAGGGTCCTGTGTGAAGCATGCTAGTGTGGTGGCTTACGTGCCTGCAAAGACTATGGCGAATGATAAGGATGAAGGGGATGTGAAATCAAGACCATTGCTTCCTATGGGGAGTGTGTATGAAGGTGCACTGTTTGTGTTGTTTGAGATGGTTGTGTATAAGTTAGGGGAGATTTTAGGTGAGAGTCCTGAAGCCGTTCGATCTCGTCATACTAATCTGGAATGA

Protein sequence

>MS.gene49065.t1

MASSSSISQVDMASMATQICNQISSIFSTPTHPHPPPLDLLLTELSTISSQNGRVFLYGVGREGLMLKALCMRLAHLGLSAHLVFDMTTPPITTNDLLIASAGPGGFSTVDAICSVARSNGGRVLLLTAQPEIGSCVKHASVVAYVPAKTMANDKDEGDVKSRPLLPMGSVYEGALFVLFEMVVYKLGEILGESPEAVRSRHTNLE