AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene59417


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59417.t1 MTR_5g021090 96.850 127 4 0 1 127 3 129 6.38e-90 256
MS.gene59417.t1 MTR_2g038560 57.547 106 45 0 19 124 16 121 3.78e-41 133
MS.gene59417.t1 MTR_2g038560 57.547 106 45 0 19 124 57 162 1.62e-40 133
MS.gene59417.t1 MTR_2g038560 57.547 106 45 0 19 124 60 165 1.69e-40 133
MS.gene59417.t1 MTR_7g035245 45.556 90 49 0 38 127 10 99 2.16e-24 90.1
MS.gene59417.t1 MTR_3g077570 43.182 88 50 0 40 127 27 114 1.32e-20 80.9
MS.gene59417.t1 MTR_1g069255 41.304 92 53 1 34 124 86 177 6.72e-20 80.9
MS.gene59417.t1 MTR_3g077560 42.353 85 49 0 40 124 30 114 2.25e-19 77.8
MS.gene59417.t1 MTR_7g108260 38.372 86 52 1 43 127 13 98 1.79e-12 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59417.t1 AT1G77370 67.500 120 39 0 8 127 11 130 2.32e-54 166
MS.gene59417.t1 AT1G77370 55.479 146 39 1 8 127 11 156 2.01e-48 152
MS.gene59417.t1 AT5G20500 51.667 120 58 0 5 124 6 125 1.33e-40 132
MS.gene59417.t1 AT5G40370 45.977 87 47 0 38 124 10 96 1.04e-22 85.9
MS.gene59417.t1 AT5G40370 48.750 80 41 0 45 124 42 121 2.05e-20 80.9
MS.gene59417.t1 AT4G28730 44.565 92 50 1 34 124 73 164 2.25e-20 81.6
MS.gene59417.t1 AT2G20270 41.304 92 53 1 34 124 78 169 5.32e-19 78.6
MS.gene59417.t1 AT5G63030 42.683 82 47 0 43 124 31 112 6.82e-18 73.9
MS.gene59417.t1 AT4G28730 45.588 68 36 1 34 100 73 140 4.45e-13 62.0
MS.gene59417.t1 AT5G14070 30.400 125 72 2 18 127 11 135 9.91e-11 55.8

Find 26 sgRNAs with CRISPR-Local

Find 87 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAAATTCAGGGTGTTCTTC+TGG 0.247136 5.4:+12909367 MS.gene59417:CDS
AGATGATGGGTATCAAATTC+AGG 0.269297 5.4:+12909355 MS.gene59417:intron
GTCCCACAAGTGTTTGTCTA+TGG 0.290060 5.4:+12909410 MS.gene59417:CDS
GGTTCCGTTCTCAAACAATA+AGG 0.298246 5.4:-12908516 None:intergenic
TACGTACGGGCAATAGGATT+TGG 0.352701 5.4:-12908606 None:intergenic
TATATATACGTACGGGCAAT+AGG 0.386425 5.4:-12908612 None:intergenic
GGTGTTCTTCTGGATTTGAT+TGG 0.395517 5.4:+12909377 MS.gene59417:CDS
GGAAACTGAATTTGAAGCTT+CGG 0.429054 5.4:-12908537 None:intergenic
GTTCCGTTCTCAAACAATAA+GGG 0.444259 5.4:-12908515 None:intergenic
TTTGTGCTTGTTTAGATGAT+GGG 0.450399 5.4:+12909342 MS.gene59417:intron
CTCAAACAATAAGGGTAAAA+CGG 0.475865 5.4:-12908507 None:intergenic
TCCCATAGACAAACACTTGT+GGG 0.486540 5.4:-12909412 None:intergenic
GATGATGGGTATCAAATTCA+GGG 0.487362 5.4:+12909356 MS.gene59417:intron
TTTGTCTATGGGAAGCATAT+TGG 0.501883 5.4:+12909422 MS.gene59417:CDS
AAATCAAGCTCAACAACAAA+AGG 0.509148 5.4:-12908758 None:intergenic
GTCTATGGGAAGCATATTGG+TGG 0.513655 5.4:+12909425 MS.gene59417:CDS
GGAGAAGATGGTGATTCTGT+TGG 0.515760 5.4:-12908585 None:intergenic
GCAATAGGATTTGGAGAAGA+TGG 0.516584 5.4:-12908597 None:intergenic
TCCCACAAGTGTTTGTCTAT+GGG 0.535106 5.4:+12909411 MS.gene59417:CDS
AAGCATATTGGTGGATCAGA+TGG 0.559694 5.4:+12909434 MS.gene59417:CDS
GTTTCTGCAATTCACCACTC+TGG 0.562376 5.4:-12910235 None:intergenic
TTACCCTTATTGTTTGAGAA+CGG 0.579036 5.4:+12908512 MS.gene59417:CDS
TTCCCATAGACAAACACTTG+TGG 0.621034 5.4:-12909413 None:intergenic
GAAGAATGAAGATGTTACAG+TGG 0.663384 5.4:+12908456 None:intergenic
GTTGTTGAGCTTGATTTGCG+AGG 0.668245 5.4:+12908764 MS.gene59417:CDS
CTCAGTGCTGCAGTCCAGAG+TGG 0.673667 5.4:+12910221 MS.gene59417:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATCTATATGTCATTATAAA+AGG + chr5.4:12910134-12910153 MS.gene59417:intron 15.0%
!!! AAGGGAATATTTATTATTTT+CGG - chr5.4:12909960-12909979 None:intergenic 15.0%
!!! TATGTGTTTTGATATTTTTA+AGG + chr5.4:12908970-12908989 MS.gene59417:intron 15.0%
!!! TTTTTTTCCATATACTTTAA+AGG + chr5.4:12910052-12910071 MS.gene59417:intron 15.0%
!! AAATAACCTATATACATAAG+AGG - chr5.4:12909067-12909086 None:intergenic 20.0%
!! AATAACCTATATACATAAGA+GGG - chr5.4:12909066-12909085 None:intergenic 20.0%
!! ATAAAAAAAAACTGCTTTCA+TGG + chr5.4:12909157-12909176 MS.gene59417:intron 20.0%
!! CTCTATATCCAAAAATATAA+GGG + chr5.4:12909874-12909893 MS.gene59417:intron 20.0%
!! TAAACTATATGAAAATAACG+AGG + chr5.4:12909821-12909840 MS.gene59417:intron 20.0%
!! TATGTCATTATAAAAGGTTA+AGG + chr5.4:12910140-12910159 MS.gene59417:intron 20.0%
!! TCATATAGTTTAAGAACTAA+AGG - chr5.4:12909813-12909832 None:intergenic 20.0%
!!! ATTAAGTTGTTTATCAAACA+AGG - chr5.4:12908833-12908852 None:intergenic 20.0%
!!! CTTATATTTTTGGATATAGA+GGG - chr5.4:12909875-12909894 None:intergenic 20.0%
!!! TATTTAGCCTTTAAAGTATA+TGG - chr5.4:12910062-12910081 None:intergenic 20.0%
!!! TGATAGAAATTTTTTATGAG+AGG + chr5.4:12909926-12909945 MS.gene59417:intron 20.0%
! AAGAGAGTTATATATACGTA+CGG - chr5.4:12908623-12908642 None:intergenic 25.0%
! AATGATAACAGTTGTCTATT+TGG - chr5.4:12909290-12909309 None:intergenic 25.0%
! ATGAAATTAATATGCTCTGT+CGG + chr5.4:12908880-12908899 MS.gene59417:intron 25.0%
! ATTCAAATAGTATACTCAGT+CGG - chr5.4:12909030-12909049 None:intergenic 25.0%
! CCTCTATATCCAAAAATATA+AGG + chr5.4:12909873-12909892 MS.gene59417:intron 25.0%
! TAATGAAGGGATTAAATTGA+AGG + chr5.4:12910095-12910114 MS.gene59417:intron 25.0%
! TATATCCAAAAATATAAGGG+TGG + chr5.4:12909877-12909896 MS.gene59417:intron 25.0%
! TATGAAAATAACGAGGAATT+TGG + chr5.4:12909828-12909847 MS.gene59417:intron 25.0%
! TCAATTATTATCACGTGTTA+GGG + chr5.4:12908649-12908668 MS.gene59417:intron 25.0%
! TTCAATTATTATCACGTGTT+AGG + chr5.4:12908648-12908667 MS.gene59417:intron 25.0%
!! ACAAATTTTCACATACTCAA+GGG - chr5.4:12909978-12909997 None:intergenic 25.0%
!! ATACTTTAAAGGCTAAATAG+AGG + chr5.4:12910063-12910082 MS.gene59417:intron 25.0%
!! TTAAGTTGTTTATCCAAACA+AGG - chr5.4:12909559-12909578 None:intergenic 25.0%
!! TTTTCATAAGTGTTTATGTC+AGG + chr5.4:12909242-12909261 MS.gene59417:intron 25.0%
!!! CCTTATATTTTTGGATATAG+AGG - chr5.4:12909876-12909895 None:intergenic 25.0%
AAATCAAGCTCAACAACAAA+AGG - chr5.4:12908761-12908780 None:intergenic 30.0%
AGAGAGTTATATATACGTAC+GGG - chr5.4:12908622-12908641 None:intergenic 30.0%
ATGAGCAAACTTTGTAAGAA+TGG - chr5.4:12910187-12910206 None:intergenic 30.0%
CATTAGTACTTAAGATGCTA+GGG - chr5.4:12910010-12910029 None:intergenic 30.0%
CTCAAACAATAAGGGTAAAA+CGG - chr5.4:12908510-12908529 None:intergenic 30.0%
CTTAAGTACTAATGAGACAA+GGG + chr5.4:12910016-12910035 MS.gene59417:intron 30.0%
TAACAGTTGTCTATTTGGAT+TGG - chr5.4:12909285-12909304 None:intergenic 30.0%
TCATTAGTACTTAAGATGCT+AGG - chr5.4:12910011-12910030 None:intergenic 30.0%
TCTTAAGTACTAATGAGACA+AGG + chr5.4:12910015-12910034 MS.gene59417:intron 30.0%
TGATACCCTCTTATGTATAT+AGG + chr5.4:12909058-12909077 MS.gene59417:intron 30.0%
TTAAGTACTAATGAGACAAG+GGG + chr5.4:12910017-12910036 MS.gene59417:intron 30.0%
TTACCCTTATTGTTTGAGAA+CGG + chr5.4:12908512-12908531 MS.gene59417:CDS 30.0%
TTTCATACTTAGTCCTTGTT+TGG + chr5.4:12909543-12909562 MS.gene59417:intron 30.0%
TTTGTGCTTGTTTAGATGAT+GGG + chr5.4:12909342-12909361 MS.gene59417:intron 30.0%
! AATGTTTCTCTGATTTTCGT+AGG + chr5.4:12908690-12908709 MS.gene59417:intron 30.0%
! ACTTTCCACCCTTATATTTT+TGG - chr5.4:12909885-12909904 None:intergenic 30.0%
! AGAGGAGATAATGATAATGA+AGG + chr5.4:12910081-12910100 MS.gene59417:intron 30.0%
! GACAAATTTTCACATACTCA+AGG - chr5.4:12909979-12909998 None:intergenic 30.0%
! GAGGAGATAATGATAATGAA+GGG + chr5.4:12910082-12910101 MS.gene59417:intron 30.0%
! TTTTGTGCTTGTTTAGATGA+TGG + chr5.4:12909341-12909360 MS.gene59417:intron 30.0%
!! ATAAGTTTTGTGAGTCTGTT+TGG - chr5.4:12909753-12909772 None:intergenic 30.0%
!! TAAGTTTTGTGAGTCTGTTT+GGG - chr5.4:12909752-12909771 None:intergenic 30.0%
!! TATATACATAAGAGGGTATC+AGG - chr5.4:12909059-12909078 None:intergenic 30.0%
!! TCTTAGACTTTTGCTTTTCT+GGG + chr5.4:12909469-12909488 MS.gene59417:intron 30.0%
!! TTCTTAGACTTTTGCTTTTC+TGG + chr5.4:12909468-12909487 MS.gene59417:intron 30.0%
ATGGATAGAGAAACACAAAG+AGG - chr5.4:12910168-12910187 None:intergenic 35.0%
GTTCCGTTCTCAAACAATAA+GGG - chr5.4:12908518-12908537 None:intergenic 35.0%
TAACGAGACAACACAATCAT+CGG - chr5.4:12908929-12908948 None:intergenic 35.0%
TATATATACGTACGGGCAAT+AGG - chr5.4:12908615-12908634 None:intergenic 35.0%
TGGATAGAGAAACACAAAGA+GGG - chr5.4:12910167-12910186 None:intergenic 35.0%
TTAAGATGCTAGGGATTAAC+AGG - chr5.4:12910001-12910020 None:intergenic 35.0%
! AAAGTTTTCGCGTAAGCTAT+AGG + chr5.4:12909095-12909114 MS.gene59417:intron 35.0%
! GGAAACTGAATTTGAAGCTT+CGG - chr5.4:12908540-12908559 None:intergenic 35.0%
! TTTGTCTATGGGAAGCATAT+TGG + chr5.4:12909422-12909441 MS.gene59417:CDS 35.0%
!! AGATGATGGGTATCAAATTC+AGG + chr5.4:12909355-12909374 MS.gene59417:intron 35.0%
!! GATGATGGGTATCAAATTCA+GGG + chr5.4:12909356-12909375 MS.gene59417:intron 35.0%
!! GATTGCGTTTTGAACAAAAG+CGG - chr5.4:12908561-12908580 None:intergenic 35.0%
GCAATAGGATTTGGAGAAGA+TGG - chr5.4:12908600-12908619 None:intergenic 40.0%
GGTGTTCTTCTGGATTTGAT+TGG + chr5.4:12909377-12909396 MS.gene59417:CDS 40.0%
GGTTCCGTTCTCAAACAATA+AGG - chr5.4:12908519-12908538 None:intergenic 40.0%
GTGGAAAGTGAGAGAAGATA+GGG + chr5.4:12909896-12909915 MS.gene59417:intron 40.0%
TCCCACAAGTGTTTGTCTAT+GGG + chr5.4:12909411-12909430 MS.gene59417:CDS 40.0%
TCCCATAGACAAACACTTGT+GGG - chr5.4:12909415-12909434 None:intergenic 40.0%
TTCCCATAGACAAACACTTG+TGG - chr5.4:12909416-12909435 None:intergenic 40.0%
! AAGCATATTGGTGGATCAGA+TGG + chr5.4:12909434-12909453 MS.gene59417:CDS 40.0%
! TCAAATTCAGGGTGTTCTTC+TGG + chr5.4:12909367-12909386 MS.gene59417:CDS 40.0%
ACTGCTTTCATGGTCTGTCA+TGG + chr5.4:12909167-12909186 MS.gene59417:intron 45.0%
AGGGATGCATGTTTGAGAGA+GGG - chr5.4:12909856-12909875 None:intergenic 45.0%
GGAGAAGATGGTGATTCTGT+TGG - chr5.4:12908588-12908607 None:intergenic 45.0%
GGTGGAAAGTGAGAGAAGAT+AGG + chr5.4:12909895-12909914 MS.gene59417:intron 45.0%
GTCCCACAAGTGTTTGTCTA+TGG + chr5.4:12909410-12909429 MS.gene59417:CDS 45.0%
GTTTCTGCAATTCACCACTC+TGG - chr5.4:12910238-12910257 None:intergenic 45.0%
TACGTACGGGCAATAGGATT+TGG - chr5.4:12908609-12908628 None:intergenic 45.0%
! GTCTATGGGAAGCATATTGG+TGG + chr5.4:12909425-12909444 MS.gene59417:CDS 45.0%
!! GTTGTTGAGCTTGATTTGCG+AGG + chr5.4:12908764-12908783 MS.gene59417:CDS 45.0%
GAGGGATGCATGTTTGAGAG+AGG - chr5.4:12909857-12909876 None:intergenic 50.0%
CTCAGTGCTGCAGTCCAGAG+TGG + chr5.4:12910221-12910240 MS.gene59417:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 12908467 12910274 12908467 ID=MS.gene59417
chr5.4 mRNA 12908467 12910274 12908467 ID=MS.gene59417.t1;Parent=MS.gene59417
chr5.4 exon 12908467 12908621 12908467 ID=MS.gene59417.t1.exon1;Parent=MS.gene59417.t1
chr5.4 CDS 12908467 12908621 12908467 ID=cds.MS.gene59417.t1;Parent=MS.gene59417.t1
chr5.4 exon 12908712 12908785 12908712 ID=MS.gene59417.t1.exon2;Parent=MS.gene59417.t1
chr5.4 CDS 12908712 12908785 12908712 ID=cds.MS.gene59417.t1;Parent=MS.gene59417.t1
chr5.4 exon 12909357 12909455 12909357 ID=MS.gene59417.t1.exon3;Parent=MS.gene59417.t1
chr5.4 CDS 12909357 12909455 12909357 ID=cds.MS.gene59417.t1;Parent=MS.gene59417.t1
chr5.4 exon 12910219 12910274 12910219 ID=MS.gene59417.t1.exon4;Parent=MS.gene59417.t1
chr5.4 CDS 12910219 12910274 12910219 ID=cds.MS.gene59417.t1;Parent=MS.gene59417.t1
Gene Sequence

>MS.gene59417

ATGTTACAGTGGAGCATGTTCATAACTCTCACGTTATTGACCGTTTTACCCTTATTGTTTGAGAACGGAACCGAAGCTTCAAATTCAGTTTCCGCTTTTGTTCAAAACGCAATCTACTCCAACAGAATCACCATCTTCTCCAAATCCTATTGCCCGTACGTATATATAACTCTCTTCAATTTTCAATTATTATCACGTGTTAGGGTTTGTTCAATTTCATCACAATGTTTCTCTGATTTTCGTAGGTATTGTTTACGTGCTAAACGTGTTTTTGTTGAACTAAATGAGCAACCTTTTGTTGTTGAGCTTGATTTGCGAGGTATCTCTTAAATCATCACTCTGTTATATTTATGTTTTGTGTCGCCTTGTTTGATAAACAACTTAATTTGCAGCTTACAGTGTAAGTGTTTTTCATGAAATTAATATGCTCTGTCGGTGTACAATATTTTACACATTCATCCGATGATTGTGTTGTCTCGTTAATTGATGAATATCACAACTTATATGTGTTTTGATATTTTTAAGGACATTATTAAATGCATGTGTAAAGTAATTGTACACCGACTGAGTATACTATTTGAATTCTTGTCCTGATACCCTCTTATGTATATAGGTTATTTCTATAACAAAAGTTTTCGCGTAAGCTATAGGCTGCTTTCATTTGCTCATAAAAAAAAATAATAAAAAAAAATAAAAAAAAACTGCTTTCATGGTCTGTCATGGAGAGCTTGTGAAAATAAGCTGAAAACAGATTATGAGCACATGTCGTACGCTGTTTTCATAAGTGTTTATGTCAGGAAATAAGTTCAAATAAGCCAATCCAAATAGACAACTGTTATCATTATTCAAACTTATCTCACGCTCGTCGTAATTTTTTTGTGCTTGTTTAGATGATGGGTATCAAATTCAGGGTGTTCTTCTGGATTTGATTGGTAGAAGAACAGTCCCACAAGTGTTTGTCTATGGGAAGCATATTGGTGGATCAGATGGTTAGTTTTTGCTTCTTAGACTTTTGCTTTTCTGGGTTACTTTTATCTATGATTAAACTCTCAATTTAGTAGTCAAGTATCACACTCTTTCATACTTAGTCCTTGTTTGGATAAACAACTTAATTTGTAGCTTTTAGTATAAGTTCGTATAAGCTATTTCTATAACAACAACAAAAAAGTTGCATTCTTTTCGTATAATCTATAAGCTGTTTTCATAAGCTATCTTGTATAGCAAATGAAAATAAGCTGAAAACAACTTATAAACAATGTCATAAGCTATTTTCATGTATTCTCCCAAACAGACTCACAAAACTTATGTTAATAGATAAGCTCAAATAAGTCAGTTCAAACAAGCCTTTAGTTCTTAAACTATATGAAAATAACGAGGAATTTGGATCCCTCTCTCAAACATGCATCCCTCTATATCCAAAAATATAAGGGTGGAAAGTGAGAGAAGATAGGGACTTAATTGATAGAAATTTTTTATGAGAGGATCTAAATCCGAAAATAATAAATATTCCCTTGAGTATGTGAAAATTTGTCCTGTTAATCCCTAGCATCTTAAGTACTAATGAGACAAGGGGCTACTTACTTTTTTTTTTTCCATATACTTTAAAGGCTAAATAGAGGAGATAATGATAATGAAGGGATTAAATTGAAGGTTTAGTGTATTGCTATTATCTATATGTCATTATAAAAGGTTAAGGTCCCTCTTTGTGTTTCTCTATCCATTCTTACAAAGTTTGCTCATTTTCTAATTTAGATCTCAGTGCTGCAGTCCAGAGTGGTGAATTGCAGAAACTTCTCAAATCAAGTTGA

Protein sequence

>MS.gene59417.t1

MLQWSMFITLTLLTVLPLLFENGTEASNSVSAFVQNAIYSNRITIFSKSYCPYCLRAKRVFVELNEQPFVVELDLRDDGYQIQGVLLDLIGRRTVPQVFVYGKHIGGSDDLSAAVQSGELQKLLKSS