Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63288.t1 | AES71020.2 | 97.7 | 565 | 13 | 0 | 1 | 565 | 51 | 615 | 0.00E+00 | 1124.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63288.t1 | F4K2A1 | 61.4 | 539 | 169 | 8 | 2 | 531 | 61 | 569 | 8.9e-180 | 632.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63288.t1 | G7J9Z6 | 97.7 | 565 | 13 | 0 | 1 | 565 | 51 | 615 | 0.0e+00 | 1124.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049150 | MS.gene63288 | 0.844006 | 9.88E-59 | -1.69E-46 |
| MS.gene049151 | MS.gene63288 | 0.831672 | 1.46E-55 | -1.69E-46 |
| MS.gene049153 | MS.gene63288 | 0.845263 | 4.53E-59 | -1.69E-46 |
| MS.gene049195 | MS.gene63288 | 0.802984 | 4.33E-49 | -1.69E-46 |
| MS.gene049297 | MS.gene63288 | 0.819958 | 8.83E-53 | -1.69E-46 |
| MS.gene04933 | MS.gene63288 | 0.82451 | 7.76E-54 | -1.69E-46 |
| MS.gene049346 | MS.gene63288 | 0.807308 | 5.39E-50 | -1.69E-46 |
| MS.gene049378 | MS.gene63288 | 0.843298 | 1.53E-58 | -1.69E-46 |
| MS.gene049426 | MS.gene63288 | 0.817823 | 2.70E-52 | -1.69E-46 |
| MS.gene049758 | MS.gene63288 | 0.834479 | 2.93E-56 | -1.69E-46 |
| MS.gene049910 | MS.gene63288 | 0.84934 | 3.44E-60 | -1.69E-46 |
| MS.gene049915 | MS.gene63288 | 0.831479 | 1.63E-55 | -1.69E-46 |
| MS.gene050226 | MS.gene63288 | 0.847504 | 1.11E-59 | -1.69E-46 |
| MS.gene050227 | MS.gene63288 | 0.847646 | 1.01E-59 | -1.69E-46 |
| MS.gene050228 | MS.gene63288 | 0.845601 | 3.67E-59 | -1.69E-46 |
| MS.gene050301 | MS.gene63288 | 0.829195 | 5.90E-55 | -1.69E-46 |
| MS.gene050442 | MS.gene63288 | 0.838601 | 2.61E-57 | -1.69E-46 |
| MS.gene050558 | MS.gene63288 | 0.838792 | 2.33E-57 | -1.69E-46 |
| MS.gene050560 | MS.gene63288 | 0.80538 | 1.37E-49 | -1.69E-46 |
| MS.gene050623 | MS.gene63288 | -0.818356 | 2.05E-52 | -1.69E-46 |
| MS.gene050642 | MS.gene63288 | 0.837072 | 6.45E-57 | -1.69E-46 |
| MS.gene050680 | MS.gene63288 | 0.802249 | 6.14E-49 | -1.69E-46 |
| MS.gene050719 | MS.gene63288 | 0.807575 | 4.73E-50 | -1.69E-46 |
| MS.gene050721 | MS.gene63288 | 0.803401 | 3.55E-49 | -1.69E-46 |
| MS.gene050724 | MS.gene63288 | 0.805835 | 1.10E-49 | -1.69E-46 |
| MS.gene050750 | MS.gene63288 | 0.811204 | 7.87E-51 | -1.69E-46 |
| MS.gene050787 | MS.gene63288 | 0.818681 | 1.73E-52 | -1.69E-46 |
| MS.gene050820 | MS.gene63288 | 0.878341 | 3.04E-69 | -1.69E-46 |
| MS.gene050824 | MS.gene63288 | 0.802861 | 4.59E-49 | -1.69E-46 |
| MS.gene050953 | MS.gene63288 | 0.804489 | 2.11E-49 | -1.69E-46 |
| MS.gene05119 | MS.gene63288 | -0.811957 | 5.40E-51 | -1.69E-46 |
| MS.gene051663 | MS.gene63288 | 0.807813 | 4.21E-50 | -1.69E-46 |
| MS.gene051860 | MS.gene63288 | 0.864844 | 9.08E-65 | -1.69E-46 |
| MS.gene051983 | MS.gene63288 | 0.858331 | 8.87E-63 | -1.69E-46 |
| MS.gene052011 | MS.gene63288 | -0.810564 | 1.08E-50 | -1.69E-46 |
| MS.gene052077 | MS.gene63288 | 0.844862 | 5.81E-59 | -1.69E-46 |
| MS.gene052176 | MS.gene63288 | 0.823694 | 1.21E-53 | -1.69E-46 |
| MS.gene052188 | MS.gene63288 | 0.807168 | 5.77E-50 | -1.69E-46 |
| MS.gene052207 | MS.gene63288 | 0.805025 | 1.63E-49 | -1.69E-46 |
| MS.gene052253 | MS.gene63288 | 0.829809 | 4.18E-55 | -1.69E-46 |
| MS.gene052255 | MS.gene63288 | 0.822111 | 2.82E-53 | -1.69E-46 |
| MS.gene052333 | MS.gene63288 | 0.809122 | 2.21E-50 | -1.69E-46 |
| MS.gene052340 | MS.gene63288 | 0.801433 | 9.03E-49 | -1.69E-46 |
| MS.gene052376 | MS.gene63288 | 0.808915 | 2.45E-50 | -1.69E-46 |
| MS.gene052389 | MS.gene63288 | 0.811971 | 5.36E-51 | -1.69E-46 |
| MS.gene053184 | MS.gene63288 | 0.808427 | 3.11E-50 | -1.69E-46 |
| MS.gene053494 | MS.gene63288 | 0.811872 | 5.63E-51 | -1.69E-46 |
| MS.gene053584 | MS.gene63288 | 0.831508 | 1.60E-55 | -1.69E-46 |
| MS.gene053836 | MS.gene63288 | 0.895615 | 8.10E-76 | -1.69E-46 |
| MS.gene054183 | MS.gene63288 | 0.819159 | 1.34E-52 | -1.69E-46 |
| MS.gene054468 | MS.gene63288 | 0.804364 | 2.24E-49 | -1.69E-46 |
| MS.gene054473 | MS.gene63288 | 0.867591 | 1.22E-65 | -1.69E-46 |
| MS.gene054542 | MS.gene63288 | 0.812023 | 5.22E-51 | -1.69E-46 |
| MS.gene054667 | MS.gene63288 | 0.808656 | 2.78E-50 | -1.69E-46 |
| MS.gene054930 | MS.gene63288 | 0.888023 | 8.52E-73 | -1.69E-46 |
| MS.gene054942 | MS.gene63288 | 0.836099 | 1.14E-56 | -1.69E-46 |
| MS.gene054983 | MS.gene63288 | 0.875998 | 1.98E-68 | -1.69E-46 |
| MS.gene05510 | MS.gene63288 | 0.80625 | 9.01E-50 | -1.69E-46 |
| MS.gene055356 | MS.gene63288 | 0.800667 | 1.29E-48 | -1.69E-46 |
| MS.gene055358 | MS.gene63288 | 0.81291 | 3.34E-51 | -1.69E-46 |
| MS.gene055367 | MS.gene63288 | 0.810893 | 9.19E-51 | -1.69E-46 |
| MS.gene055422 | MS.gene63288 | 0.800672 | 1.29E-48 | -1.69E-46 |
| MS.gene05553 | MS.gene63288 | 0.822612 | 2.16E-53 | -1.69E-46 |
| MS.gene055701 | MS.gene63288 | 0.817264 | 3.61E-52 | -1.69E-46 |
| MS.gene055859 | MS.gene63288 | 0.830993 | 2.15E-55 | -1.69E-46 |
| MS.gene056506 | MS.gene63288 | 0.811122 | 8.20E-51 | -1.69E-46 |
| MS.gene056612 | MS.gene63288 | 0.801071 | 1.07E-48 | -1.69E-46 |
| MS.gene056723 | MS.gene63288 | 0.844851 | 5.85E-59 | -1.69E-46 |
| MS.gene056875 | MS.gene63288 | 0.810315 | 1.23E-50 | -1.69E-46 |
| MS.gene056979 | MS.gene63288 | 0.846956 | 1.57E-59 | -1.69E-46 |
| MS.gene057121 | MS.gene63288 | 0.86205 | 6.67E-64 | -1.69E-46 |
| MS.gene057245 | MS.gene63288 | 0.818208 | 2.21E-52 | -1.69E-46 |
| MS.gene057448 | MS.gene63288 | 0.806561 | 7.75E-50 | -1.69E-46 |
| MS.gene057469 | MS.gene63288 | 0.855087 | 7.98E-62 | -1.69E-46 |
| MS.gene05761 | MS.gene63288 | 0.816308 | 5.91E-52 | -1.69E-46 |
| MS.gene057639 | MS.gene63288 | 0.807465 | 4.99E-50 | -1.69E-46 |
| MS.gene057750 | MS.gene63288 | 0.837536 | 4.91E-57 | -1.69E-46 |
| MS.gene057967 | MS.gene63288 | 0.80781 | 4.21E-50 | -1.69E-46 |
| MS.gene058002 | MS.gene63288 | 0.857411 | 1.66E-62 | -1.69E-46 |
| MS.gene05811 | MS.gene63288 | 0.839603 | 1.44E-57 | -1.69E-46 |
| MS.gene058142 | MS.gene63288 | 0.842403 | 2.64E-58 | -1.69E-46 |
| MS.gene058710 | MS.gene63288 | 0.869152 | 3.84E-66 | -1.69E-46 |
| MS.gene058736 | MS.gene63288 | 0.815728 | 7.97E-52 | -1.69E-46 |
| MS.gene058739 | MS.gene63288 | 0.837243 | 5.83E-57 | -1.69E-46 |
| MS.gene058749 | MS.gene63288 | 0.837875 | 4.01E-57 | -1.69E-46 |
| MS.gene058833 | MS.gene63288 | 0.816731 | 4.75E-52 | -1.69E-46 |
| MS.gene058845 | MS.gene63288 | 0.802784 | 4.76E-49 | -1.69E-46 |
| MS.gene05907 | MS.gene63288 | 0.840559 | 8.08E-58 | -1.69E-46 |
| MS.gene059124 | MS.gene63288 | 0.803778 | 2.96E-49 | -1.69E-46 |
| MS.gene059128 | MS.gene63288 | 0.822137 | 2.78E-53 | -1.69E-46 |
| MS.gene059285 | MS.gene63288 | 0.83605 | 1.17E-56 | -1.69E-46 |
| MS.gene059420 | MS.gene63288 | 0.806549 | 7.80E-50 | -1.69E-46 |
| MS.gene05984 | MS.gene63288 | 0.854329 | 1.32E-61 | -1.69E-46 |
| MS.gene059899 | MS.gene63288 | 0.805364 | 1.38E-49 | -1.69E-46 |
| MS.gene060683 | MS.gene63288 | 0.80013 | 1.66E-48 | -1.69E-46 |
| MS.gene060998 | MS.gene63288 | 0.813738 | 2.20E-51 | -1.69E-46 |
| MS.gene061040 | MS.gene63288 | -0.802508 | 5.43E-49 | -1.69E-46 |
| MS.gene061049 | MS.gene63288 | 0.838934 | 2.14E-57 | -1.69E-46 |
| MS.gene061114 | MS.gene63288 | -0.84979 | 2.58E-60 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63288.t1 | MTR_3g069180 | 97.527 | 566 | 14 | 0 | 1 | 566 | 51 | 616 | 0.0 | 1156 |
| MS.gene63288.t1 | MTR_3g069160 | 97.928 | 531 | 11 | 0 | 1 | 531 | 1 | 531 | 0.0 | 1090 |
| MS.gene63288.t1 | MTR_3g069160 | 94.891 | 548 | 11 | 1 | 1 | 531 | 51 | 598 | 0.0 | 1080 |
| MS.gene63288.t1 | MTR_5g081250 | 76.438 | 539 | 116 | 6 | 3 | 531 | 4 | 541 | 0.0 | 837 |
| MS.gene63288.t1 | MTR_3g069160 | 98.481 | 395 | 6 | 0 | 137 | 531 | 1 | 395 | 0.0 | 810 |
| MS.gene63288.t1 | MTR_5g081250 | 76.291 | 523 | 113 | 6 | 3 | 515 | 4 | 525 | 0.0 | 810 |
| MS.gene63288.t1 | MTR_3g069150 | 98.168 | 273 | 5 | 0 | 573 | 845 | 1 | 273 | 0.0 | 552 |
| MS.gene63288.t1 | MTR_1g051385 | 51.950 | 564 | 232 | 11 | 6 | 531 | 56 | 618 | 0.0 | 541 |
| MS.gene63288.t1 | MTR_1g051385 | 52.987 | 519 | 208 | 10 | 48 | 531 | 4 | 521 | 1.21e-175 | 517 |
| MS.gene63288.t1 | MTR_3g069170 | 97.917 | 240 | 5 | 0 | 573 | 812 | 1 | 240 | 4.15e-169 | 489 |
| MS.gene63288.t1 | MTR_3g069150 | 98.104 | 211 | 4 | 0 | 573 | 783 | 1 | 211 | 1.93e-148 | 437 |
| MS.gene63288.t1 | MTR_5g081040 | 74.725 | 273 | 67 | 2 | 573 | 844 | 1 | 272 | 1.40e-142 | 422 |
| MS.gene63288.t1 | MTR_5g081040 | 80.383 | 209 | 40 | 1 | 637 | 844 | 4 | 212 | 6.77e-117 | 353 |
| MS.gene63288.t1 | MTR_7g113620 | 40.000 | 215 | 121 | 3 | 609 | 817 | 36 | 248 | 6.03e-44 | 160 |
| MS.gene63288.t1 | MTR_1g028510 | 30.448 | 335 | 178 | 9 | 58 | 352 | 84 | 403 | 9.72e-32 | 131 |
| MS.gene63288.t1 | MTR_1g028510 | 30.189 | 318 | 167 | 9 | 58 | 335 | 84 | 386 | 9.25e-29 | 119 |
| MS.gene63288.t1 | MTR_7g100940 | 27.046 | 281 | 151 | 8 | 81 | 322 | 103 | 368 | 1.05e-19 | 92.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63288.t1 | AT5G05980 | 62.338 | 539 | 164 | 8 | 2 | 531 | 61 | 569 | 0.0 | 668 |
| MS.gene63288.t1 | AT5G05980 | 62.338 | 539 | 164 | 8 | 2 | 531 | 3 | 511 | 0.0 | 668 |
| MS.gene63288.t1 | AT3G55630 | 58.083 | 532 | 180 | 10 | 1 | 531 | 1 | 490 | 0.0 | 599 |
| MS.gene63288.t1 | AT3G55630 | 57.895 | 532 | 180 | 11 | 1 | 531 | 1 | 489 | 0.0 | 593 |
| MS.gene63288.t1 | AT3G55630 | 55.075 | 532 | 174 | 11 | 1 | 531 | 1 | 468 | 0.0 | 552 |
| MS.gene63288.t1 | AT3G10160 | 51.607 | 560 | 235 | 10 | 2 | 531 | 70 | 623 | 0.0 | 547 |
| MS.gene63288.t1 | AT3G55630 | 58.774 | 473 | 154 | 9 | 60 | 531 | 1 | 433 | 0.0 | 547 |
| MS.gene63288.t1 | AT3G55630 | 58.774 | 473 | 154 | 9 | 60 | 531 | 1 | 433 | 0.0 | 547 |
| MS.gene63288.t1 | AT3G55630 | 58.774 | 473 | 154 | 9 | 60 | 531 | 1 | 433 | 0.0 | 547 |
| MS.gene63288.t1 | AT3G55630 | 55.808 | 396 | 134 | 9 | 137 | 531 | 1 | 356 | 8.02e-141 | 421 |
| MS.gene63288.t1 | AT2G39805 | 60.791 | 278 | 97 | 3 | 576 | 845 | 2 | 275 | 9.24e-110 | 337 |
| MS.gene63288.t1 | AT2G39805 | 60.573 | 279 | 97 | 4 | 576 | 845 | 2 | 276 | 5.57e-108 | 333 |
| MS.gene63288.t1 | AT5G27490 | 38.434 | 281 | 154 | 7 | 577 | 844 | 4 | 278 | 3.15e-50 | 178 |
| MS.gene63288.t1 | AT3G05280 | 35.821 | 268 | 156 | 6 | 573 | 830 | 1 | 262 | 1.04e-43 | 160 |
| MS.gene63288.t1 | AT5G41480 | 37.500 | 168 | 103 | 1 | 50 | 215 | 84 | 251 | 1.86e-29 | 124 |
Find 176 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTACTAAATGGTTTAAAATT+AGG | 0.057059 | 3.3:-60608051 | MS.gene63288:CDS |
| GCACATGCTTCCATGCTTTC+AGG | 0.165417 | 3.3:+60607681 | None:intergenic |
| TCTTCATATGGCGATGATTT+TGG | 0.165761 | 3.3:+60613935 | None:intergenic |
| CGTCTTATTCGAGTACTTAA+AGG | 0.176550 | 3.3:-60613837 | MS.gene63288:intron |
| AATTAATCGTAGCGATTATT+TGG | 0.193578 | 3.3:-60603596 | MS.gene63288:intron |
| TACTAAATGGTTTAAAATTA+GGG | 0.198474 | 3.3:-60608050 | MS.gene63288:CDS |
| AAACCAGTCAACTCGCTTTC+AGG | 0.213072 | 3.3:-60605715 | MS.gene63288:intron |
| TTCATCCATGCAGATGCTTT+TGG | 0.236864 | 3.3:-60602457 | MS.gene63288:intron |
| ATTACAGGTATGGGCTTATA+TGG | 0.245624 | 3.3:-60601411 | MS.gene63288:intron |
| CGAGAAAGATTTCGTTTAGA+TGG | 0.260770 | 3.3:-60613018 | MS.gene63288:CDS |
| ACTGGTTCCATACATCTTAT+TGG | 0.265122 | 3.3:-60605424 | MS.gene63288:CDS |
| ATGCTAACCTTATACGGTTT+TGG | 0.272157 | 3.3:-60601195 | MS.gene63288:CDS |
| TTAGATGCCAAATTACTAAA+TGG | 0.272407 | 3.3:-60608063 | MS.gene63288:CDS |
| CTGGAACTGATCCGAGTAAA+TGG | 0.284659 | 3.3:+60603526 | None:intergenic |
| CGGAGGCGGAGGCGGACAAC+AGG | 0.291792 | 3.3:-60603098 | MS.gene63288:CDS |
| TATTGGTGATGTGTTGAAAT+TGG | 0.304394 | 3.3:-60605407 | MS.gene63288:intron |
| GTTCAAATGCTAACCTTATA+CGG | 0.310617 | 3.3:-60601201 | MS.gene63288:CDS |
| ATGCATCAGGTCCTTGTAAC+TGG | 0.311721 | 3.3:-60605442 | MS.gene63288:intron |
| TGAGAAGGCGGGAATCTTAA+AGG | 0.312541 | 3.3:-60609800 | MS.gene63288:intron |
| GATTATTTGGTTTCTTTCTA+TGG | 0.312608 | 3.3:-60603583 | MS.gene63288:CDS |
| AGTTCACTCTACAAAGAGTA+TGG | 0.322540 | 3.3:-60606042 | MS.gene63288:CDS |
| TCTACATGGATGAATATAAA+TGG | 0.327429 | 3.3:-60607976 | MS.gene63288:CDS |
| TAAAGCAAGGAAGTGGAAAA+AGG | 0.337269 | 3.3:+60610666 | None:intergenic |
| CACCACTTTATTTGAGAACT+TGG | 0.339731 | 3.3:+60607593 | None:intergenic |
| CATTGCCACTGGTTATCAAT+TGG | 0.345970 | 3.3:-60605758 | MS.gene63288:CDS |
| ACAAGCACCAAAACCGTATA+AGG | 0.349509 | 3.3:+60601188 | None:intergenic |
| TACAGAAGACAAGATGAAAA+TGG | 0.358922 | 3.3:+60601150 | None:intergenic |
| TGGACATTTGATGGATTCAT+TGG | 0.366252 | 3.3:-60605205 | MS.gene63288:CDS |
| GCTTCCAATGCTTCTTCATA+TGG | 0.369429 | 3.3:+60613923 | None:intergenic |
| TATGACCACATGGAAATTCT+TGG | 0.370689 | 3.3:-60610283 | MS.gene63288:intron |
| TGCATAATGGAGGTTGGGTT+AGG | 0.370944 | 3.3:-60610500 | MS.gene63288:CDS |
| CACTGGTTATCAATTGGCTT+AGG | 0.371537 | 3.3:-60605752 | MS.gene63288:CDS |
| ATATTTACAGAAGGAGATAA+TGG | 0.371821 | 3.3:-60613962 | MS.gene63288:intron |
| TCCGCCTCCGCCTCCACTGT+TGG | 0.372021 | 3.3:+60603111 | None:intergenic |
| ACGGCTGCATGGTCTATATA+TGG | 0.373238 | 3.3:-60601274 | MS.gene63288:CDS |
| AGTGGAGGCGGAGGCGGAGG+CGG | 0.373465 | 3.3:-60603106 | MS.gene63288:CDS |
| TTGAACATTATCCATGTTGC+TGG | 0.382054 | 3.3:-60613548 | MS.gene63288:CDS |
| GATGTTTGCATAATGGAGGT+TGG | 0.388180 | 3.3:-60610506 | MS.gene63288:CDS |
| TTATCAATGTACCACAAGAT+TGG | 0.388558 | 3.3:-60606112 | MS.gene63288:CDS |
| CCGCCTCCGCCTCCACTGTT+GGG | 0.392180 | 3.3:+60603112 | None:intergenic |
| AATGTTTGCATATTTGCATA+AGG | 0.396825 | 3.3:+60603139 | None:intergenic |
| GAAGTTATACCACACACAAT+AGG | 0.399740 | 3.3:+60610313 | None:intergenic |
| TAATAAAAGTACCTTCCCTT+TGG | 0.401887 | 3.3:+60613523 | None:intergenic |
| GGCAACAGCCAGCTAATAAC+TGG | 0.407158 | 3.3:-60602435 | MS.gene63288:CDS |
| AGTACATCCATTGCTTCATC+AGG | 0.408417 | 3.3:+60608903 | None:intergenic |
| CTCTTCTGAAAATATCTTGA+AGG | 0.410763 | 3.3:+60610639 | None:intergenic |
| CCCAACAGTGGAGGCGGAGG+CGG | 0.414258 | 3.3:-60603112 | MS.gene63288:CDS |
| TCGTGTTGGTGATGTCAATA+TGG | 0.419030 | 3.3:-60613873 | MS.gene63288:CDS |
| CAAACTCTTGGAACTCCAAC+TGG | 0.420287 | 3.3:-60603070 | MS.gene63288:intron |
| CGTTGCAAAATTTCCAAGTA+AGG | 0.421093 | 3.3:+60601360 | None:intergenic |
| GTGTGTGGTATAACTTCTCT+TGG | 0.421453 | 3.3:-60610307 | MS.gene63288:CDS |
| ACAGGTGGATGTTTGCATAA+TGG | 0.421945 | 3.3:-60610513 | MS.gene63288:intron |
| AACAGTGGAGGCGGAGGCGG+AGG | 0.422692 | 3.3:-60603109 | MS.gene63288:CDS |
| GCAGCTCATTGAGAGTGGTT+TGG | 0.424737 | 3.3:-60605272 | MS.gene63288:intron |
| TTATTCCAGTTGAGATTCTT+CGG | 0.425394 | 3.3:-60600951 | MS.gene63288:CDS |
| TATTTACAGAAGGAGATAAT+GGG | 0.426485 | 3.3:-60613961 | MS.gene63288:intron |
| GGTTTAAAATTAGGGCTTGA+AGG | 0.427252 | 3.3:-60608042 | MS.gene63288:CDS |
| ATGTTTGCATATTTGCATAA+GGG | 0.429062 | 3.3:+60603140 | None:intergenic |
| GATGAAACAAGGTCTGGTTC+TGG | 0.433364 | 3.3:+60607631 | None:intergenic |
| CACATCACCAATAAGATGTA+TGG | 0.435776 | 3.3:+60605417 | None:intergenic |
| CAAGACCAGCATTAACATAT+TGG | 0.438258 | 3.3:+60608013 | None:intergenic |
| CTGTTGCATTATGCTCTACA+TGG | 0.446804 | 3.3:-60607990 | MS.gene63288:CDS |
| TGGATCATAATAATCCTTAC+CGG | 0.450308 | 3.3:-60600928 | MS.gene63288:CDS |
| TGATGTTAGCTATGTGAATA+CGG | 0.450659 | 3.3:-60601293 | MS.gene63288:CDS |
| TTGCCAGAGCAGTTCATAAA+CGG | 0.451738 | 3.3:-60607810 | MS.gene63288:CDS |
| ATCCACCGAAGAATCTCAAC+TGG | 0.453879 | 3.3:+60600946 | None:intergenic |
| GGTTCTGGTCTTTGATTGCA+AGG | 0.458482 | 3.3:+60607646 | None:intergenic |
| TATATATGGCTATGTGATAG+TGG | 0.458907 | 3.3:-60601260 | MS.gene63288:CDS |
| ACTGGTTATCAATTGGCTTA+GGG | 0.461528 | 3.3:-60605751 | MS.gene63288:CDS |
| TTCTGAAGAATTAGCTGGTA+TGG | 0.465934 | 3.3:-60605823 | MS.gene63288:CDS |
| AAATGCATAACTCACAGATC+AGG | 0.466971 | 3.3:+60602286 | None:intergenic |
| GTCATCACCGACTGGATTCA+AGG | 0.474193 | 3.3:+60602333 | None:intergenic |
| AAGAAGGCGCTATTTGTGCC+TGG | 0.475535 | 3.3:-60606136 | MS.gene63288:CDS |
| AATCCTTGCCAGTTATTAGC+TGG | 0.478284 | 3.3:+60602427 | None:intergenic |
| AATACTCTTGGAGAAATTGC+TGG | 0.484435 | 3.3:-60609823 | MS.gene63288:CDS |
| ATGTTTGCATAATGGAGGTT+GGG | 0.485602 | 3.3:-60610505 | MS.gene63288:CDS |
| GAGAAAGATTTCGTTTAGAT+GGG | 0.487035 | 3.3:-60613017 | MS.gene63288:intron |
| CAACTGCAAGTCTGCAAGGA+AGG | 0.487658 | 3.3:-60607782 | MS.gene63288:CDS |
| ATATTAACAGGGAATACTCT+TGG | 0.488965 | 3.3:-60609835 | MS.gene63288:intron |
| GATTTGGTAGATGCTTGAAT+TGG | 0.490018 | 3.3:-60613588 | MS.gene63288:intron |
| CTCCGCCTCCACTGTTGGGA+GGG | 0.497926 | 3.3:+60603116 | None:intergenic |
| CTATCTGATGAAACAAGGTC+TGG | 0.499454 | 3.3:+60607625 | None:intergenic |
| ATGAAAACTTGTGCTGATCA+TGG | 0.502432 | 3.3:-60607101 | MS.gene63288:intron |
| TGTGTGGTATAACTTCTCTT+GGG | 0.503285 | 3.3:-60610306 | MS.gene63288:CDS |
| AAACCGTTTATGAACTGCTC+TGG | 0.505379 | 3.3:+60607807 | None:intergenic |
| CAACAGGGATATCAAACTCT+TGG | 0.506858 | 3.3:-60603082 | MS.gene63288:CDS |
| TTCCCTCCCAACAGTGGAGG+CGG | 0.507299 | 3.3:-60603118 | MS.gene63288:CDS |
| TTCCAAGTTCTCAAATAAAG+TGG | 0.507300 | 3.3:-60607595 | MS.gene63288:CDS |
| ATCCATGTTGCTGGTACCAA+AGG | 0.510879 | 3.3:-60613539 | MS.gene63288:CDS |
| TTTAGTAATTTGGCATCTAA+CGG | 0.512015 | 3.3:+60608066 | None:intergenic |
| GGTTGAGTCACTGTAAATGC+TGG | 0.513165 | 3.3:+60608924 | None:intergenic |
| CCTCCCAACAGTGGAGGCGG+AGG | 0.514348 | 3.3:-60603115 | MS.gene63288:CDS |
| CCTCCGCCTCCACTGTTGGG+AGG | 0.519513 | 3.3:+60603115 | None:intergenic |
| CGTTCGCAGCTCATTGAGAG+TGG | 0.522937 | 3.3:-60605277 | MS.gene63288:intron |
| TAGCTGAGGCAGCACCGGTA+AGG | 0.526708 | 3.3:+60600914 | None:intergenic |
| TTACCTGAAAGCGAGTTGAC+TGG | 0.526726 | 3.3:+60605712 | None:intergenic |
| AAGAATGCTTCAAACAATCA+AGG | 0.526990 | 3.3:-60603356 | MS.gene63288:intron |
| TTAGATGAGGAGAAGTGAAA+AGG | 0.531483 | 3.3:+60613049 | None:intergenic |
| ATCGCCATATGAAGAAGCAT+TGG | 0.533099 | 3.3:-60613927 | MS.gene63288:CDS |
| GAAATTGCTGGTGAGAAGGC+GGG | 0.535726 | 3.3:-60609811 | MS.gene63288:CDS |
| AACGGAGATGTTACTTGAAG+AGG | 0.538384 | 3.3:+60608084 | None:intergenic |
| GTAGATGCTTGAATTGGAAG+AGG | 0.539812 | 3.3:-60613582 | MS.gene63288:intron |
| GTAGAGTGAACTGCCATGAC+AGG | 0.542280 | 3.3:+60606053 | None:intergenic |
| AAAGCTAGCTGAGGCAGCAC+CGG | 0.543010 | 3.3:+60600909 | None:intergenic |
| ATGGCTTGTATTGCTGCTAC+TGG | 0.544057 | 3.3:+60603545 | None:intergenic |
| GCTTTGTTTCTCTGAACCTA+CGG | 0.544632 | 3.3:-60600891 | MS.gene63288:CDS |
| GGTTTACATCAGCATCAGCT+TGG | 0.546102 | 3.3:-60605184 | MS.gene63288:intron |
| TATATGGATCTCAACAACAT+TGG | 0.547591 | 3.3:-60601395 | MS.gene63288:CDS |
| ATTACTAAACGCACTCGTGT+TGG | 0.548955 | 3.3:-60613887 | MS.gene63288:CDS |
| GGAGGCGGAGGCGGACAACA+GGG | 0.549463 | 3.3:-60603097 | MS.gene63288:CDS |
| AACTGCAAGTCTGCAAGGAA+GGG | 0.551072 | 3.3:-60607781 | MS.gene63288:CDS |
| TGGAGAAATTGCTGGTGAGA+AGG | 0.551632 | 3.3:-60609815 | MS.gene63288:CDS |
| GGCTCATAGTCCTGAAAGCA+TGG | 0.554574 | 3.3:-60607691 | MS.gene63288:CDS |
| AAATTGAATACCTTGAGCCA+TGG | 0.556713 | 3.3:+60614088 | None:intergenic |
| GCAGCTTCTGAAGAATTAGC+TGG | 0.558289 | 3.3:-60605828 | MS.gene63288:CDS |
| AGGCAAGTAAAGCAAGGAAG+TGG | 0.560496 | 3.3:+60610659 | None:intergenic |
| GAGCACCAATATGTTAATGC+TGG | 0.560675 | 3.3:-60608018 | MS.gene63288:CDS |
| AATGGATGTACTTAAAGAGA+AGG | 0.562231 | 3.3:-60608892 | MS.gene63288:CDS |
| TCCATGTTGCTGGTACCAAA+GGG | 0.562914 | 3.3:-60613538 | MS.gene63288:CDS |
| TCAAATGAGACTCTTCAAGA+TGG | 0.563572 | 3.3:+60607953 | None:intergenic |
| GTATGGGAAAATCTCATGCA+AGG | 0.565634 | 3.3:-60606025 | MS.gene63288:CDS |
| GATGTATGGAACCAGTTACA+AGG | 0.566973 | 3.3:+60605431 | None:intergenic |
| ATAATGGAGGTTGGGTTAGG+TGG | 0.568173 | 3.3:-60610497 | MS.gene63288:CDS |
| TCCCTTTGGTACCAGCAACA+TGG | 0.570504 | 3.3:+60613537 | None:intergenic |
| TATAGGAAATGATCTTTCAG+TGG | 0.570515 | 3.3:-60600863 | MS.gene63288:CDS |
| AATGCATAACTCACAGATCA+GGG | 0.570733 | 3.3:+60602287 | None:intergenic |
| ATTCTAACATTGACCTGTCA+TGG | 0.572664 | 3.3:-60606066 | MS.gene63288:CDS |
| GTGGTGTTATGATAGACTAA+AGG | 0.574335 | 3.3:-60611481 | MS.gene63288:intron |
| TTAACAACTGCAAGTCTGCA+AGG | 0.586475 | 3.3:-60607786 | MS.gene63288:CDS |
| TCCAAATTAACCATGGCTCA+AGG | 0.586683 | 3.3:-60614098 | None:intergenic |
| GACTCAACCTGATGAAGCAA+TGG | 0.590492 | 3.3:-60608910 | MS.gene63288:CDS |
| GTTCACTCTACAAAGAGTAT+GGG | 0.590549 | 3.3:-60606041 | MS.gene63288:CDS |
| TTCTCTTGGGTATGACCACA+TGG | 0.591494 | 3.3:-60610293 | MS.gene63288:CDS |
| GTTCTGGTCTTTGATTGCAA+GGG | 0.592376 | 3.3:+60607647 | None:intergenic |
| AATCTCATGCAAGGGAACAA+AGG | 0.592939 | 3.3:-60606016 | MS.gene63288:intron |
| AAGACAAGATGAAAATGGTG+AGG | 0.598153 | 3.3:+60601155 | None:intergenic |
| CAAATACGATGCAACAAATG+TGG | 0.602729 | 3.3:-60610474 | MS.gene63288:intron |
| CAGCCAGCTAATAACTGGCA+AGG | 0.605206 | 3.3:-60602430 | MS.gene63288:CDS |
| AGAAATTGCTGGTGAGAAGG+CGG | 0.607678 | 3.3:-60609812 | MS.gene63288:CDS |
| TGCAGGTGTGTACTTCAAGA+AGG | 0.607814 | 3.3:-60606152 | MS.gene63288:intron |
| TATTTGGTTTCTTTCTATGG+AGG | 0.609962 | 3.3:-60603580 | MS.gene63288:CDS |
| AGACAAGATGAAAATGGTGA+GGG | 0.610681 | 3.3:+60601156 | None:intergenic |
| AGCAATACAAGCCATTTACT+CGG | 0.612006 | 3.3:-60603537 | MS.gene63288:CDS |
| GCTATGTGAATACGGCTGCA+TGG | 0.612811 | 3.3:-60601285 | MS.gene63288:CDS |
| CTATGTGATAGTGGTCCCAT+TGG | 0.614198 | 3.3:-60601251 | MS.gene63288:CDS |
| ATACAGATAACAGAACATCA+TGG | 0.618085 | 3.3:-60601321 | MS.gene63288:CDS |
| ATAAGTTCCTTGAATCCAGT+CGG | 0.621033 | 3.3:-60602340 | MS.gene63288:CDS |
| TCTTGAAGGCAAGTAAAGCA+AGG | 0.621402 | 3.3:+60610653 | None:intergenic |
| TCTCGTATATCAATTAGATG+AGG | 0.624514 | 3.3:+60613036 | None:intergenic |
| TATGGGAAAATCTCATGCAA+GGG | 0.625677 | 3.3:-60606024 | MS.gene63288:CDS |
| CATTTGAACCCATATACTGA+AGG | 0.626704 | 3.3:+60601215 | None:intergenic |
| ATCAGCTATCTGATGAAACA+AGG | 0.631446 | 3.3:+60607620 | None:intergenic |
| CAGAGAAACAAAGCTAGCTG+AGG | 0.631465 | 3.3:+60600900 | None:intergenic |
| TTCCAGTTGAGATTCTTCGG+TGG | 0.636030 | 3.3:-60600948 | MS.gene63288:CDS |
| ATTGATAACCAGTGGCAATG+AGG | 0.637607 | 3.3:+60605761 | None:intergenic |
| GAACTGAACTTTGATGAAGA+CGG | 0.639176 | 3.3:+60600804 | None:intergenic |
| GCATGGAAGCATGTGCGAAG+TGG | 0.640363 | 3.3:-60607674 | MS.gene63288:CDS |
| ACATTCCCTCCCAACAGTGG+AGG | 0.642219 | 3.3:-60603121 | MS.gene63288:CDS |
| AGGTCAATGTTAGAATCACT+TGG | 0.645779 | 3.3:+60606073 | None:intergenic |
| CAAACATTCCCTCCCAACAG+TGG | 0.649727 | 3.3:-60603124 | MS.gene63288:CDS |
| TTTAGTAATCAAAGATGACA+AGG | 0.654793 | 3.3:+60613901 | None:intergenic |
| AAGCATGAGATCCAATCTTG+TGG | 0.655306 | 3.3:+60606101 | None:intergenic |
| CATTTCCTATAAAAGACCGT+AGG | 0.655329 | 3.3:+60600875 | None:intergenic |
| TGTTGCTGGTACCAAAGGGA+AGG | 0.657751 | 3.3:-60613534 | MS.gene63288:intron |
| GAATCTATATTACGCAACTG+TGG | 0.657927 | 3.3:-60613084 | MS.gene63288:CDS |
| GCGTAATATAGATTCTGCGA+AGG | 0.659957 | 3.3:+60613092 | None:intergenic |
| CTAAGCCAATTGATAACCAG+TGG | 0.666215 | 3.3:+60605753 | None:intergenic |
| TTGTGGTACATTGATAAACC+AGG | 0.673003 | 3.3:+60606118 | None:intergenic |
| AAAGTGGTGAAGACGCACCA+AGG | 0.676464 | 3.3:-60607579 | MS.gene63288:CDS |
| GGTGGATGTTTGCATAATGG+AGG | 0.684054 | 3.3:-60610510 | MS.gene63288:CDS |
| AAGTGAGGAAGAAGTAACTG+AGG | 0.686138 | 3.3:+60607125 | None:intergenic |
| TGTTGGTGATGTCAATATGG+AGG | 0.724403 | 3.3:-60613870 | MS.gene63288:CDS |
| TTAAGTACTCGAATAAGACG+AGG | 0.728653 | 3.3:+60613840 | None:intergenic |
| GTGCAGAAACCTATTGTGTG+TGG | 0.729317 | 3.3:-60610322 | MS.gene63288:CDS |
| ACTAACCAAGAATTTCCATG+TGG | 0.790649 | 3.3:+60610278 | None:intergenic |
| GTACACACAGTATTTCAACG+TGG | 0.852750 | 3.3:-60602389 | MS.gene63288:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 60600795 | 60614108 | 60600795 | ID=MS.gene63288 |
| chr3.3 | mRNA | 60600795 | 60614108 | 60600795 | ID=MS.gene63288.t1;Parent=MS.gene63288 |
| chr3.3 | exon | 60614099 | 60614108 | 60614099 | ID=MS.gene63288.t1.exon1;Parent=MS.gene63288.t1 |
| chr3.3 | CDS | 60614099 | 60614108 | 60614099 | ID=cds.MS.gene63288.t1;Parent=MS.gene63288.t1 |
| chr3.3 | exon | 60613838 | 60613974 | 60613838 | ID=MS.gene63288.t1.exon2;Parent=MS.gene63288.t1 |
| chr3.3 | CDS | 60613838 | 60613974 | 60613838 | ID=cds.MS.gene63288.t1;Parent=MS.gene63288.t1 |
| chr3.3 | exon | 60613535 | 60613600 | 60613535 | ID=MS.gene63288.t1.exon3;Parent=MS.gene63288.t1 |
| chr3.3 | CDS | 60613535 | 60613600 | 60613535 | ID=cds.MS.gene63288.t1;Parent=MS.gene63288.t1 |
| chr3.3 | exon | 60613018 | 60613127 | 60613018 | ID=MS.gene63288.t1.exon4;Parent=MS.gene63288.t1 |
| chr3.3 | CDS | 60613018 | 60613127 | 60613018 | ID=cds.MS.gene63288.t1;Parent=MS.gene63288.t1 |
| chr3.3 | exon | 60611482 | 60611542 | 60611482 | ID=MS.gene63288.t1.exon5;Parent=MS.gene63288.t1 |
| chr3.3 | CDS | 60611482 | 60611542 | 60611482 | ID=cds.MS.gene63288.t1;Parent=MS.gene63288.t1 |
| chr3.3 | exon | 60610636 | 60610719 | 60610636 | ID=MS.gene63288.t1.exon6;Parent=MS.gene63288.t1 |
| chr3.3 | CDS | 60610636 | 60610719 | 60610636 | ID=cds.MS.gene63288.t1;Parent=MS.gene63288.t1 |
| chr3.3 | exon | 60610475 | 60610531 | 60610475 | ID=MS.gene63288.t1.exon7;Parent=MS.gene63288.t1 |
| chr3.3 | CDS | 60610475 | 60610531 | 60610475 | ID=cds.MS.gene63288.t1;Parent=MS.gene63288.t1 |
| chr3.3 | exon | 60610284 | 60610344 | 60610284 | ID=MS.gene63288.t1.exon8;Parent=MS.gene63288.t1 |
| chr3.3 | CDS | 60610284 | 60610344 | 60610284 | ID=cds.MS.gene63288.t1;Parent=MS.gene63288.t1 |
| chr3.3 | exon | 60609801 | 60609847 | 60609801 | ID=MS.gene63288.t1.exon9;Parent=MS.gene63288.t1 |
| chr3.3 | CDS | 60609801 | 60609847 | 60609801 | ID=cds.MS.gene63288.t1;Parent=MS.gene63288.t1 |
| chr3.3 | exon | 60608878 | 60608955 | 60608878 | ID=MS.gene63288.t1.exon10;Parent=MS.gene63288.t1 |
| chr3.3 | CDS | 60608878 | 60608955 | 60608878 | ID=cds.MS.gene63288.t1;Parent=MS.gene63288.t1 |
| chr3.3 | exon | 60607945 | 60608109 | 60607945 | ID=MS.gene63288.t1.exon11;Parent=MS.gene63288.t1 |
| chr3.3 | CDS | 60607945 | 60608109 | 60607945 | ID=cds.MS.gene63288.t1;Parent=MS.gene63288.t1 |
| chr3.3 | exon | 60607557 | 60607841 | 60607557 | ID=MS.gene63288.t1.exon12;Parent=MS.gene63288.t1 |
| chr3.3 | CDS | 60607557 | 60607841 | 60607557 | ID=cds.MS.gene63288.t1;Parent=MS.gene63288.t1 |
| chr3.3 | exon | 60607102 | 60607180 | 60607102 | ID=MS.gene63288.t1.exon13;Parent=MS.gene63288.t1 |
| chr3.3 | CDS | 60607102 | 60607180 | 60607102 | ID=cds.MS.gene63288.t1;Parent=MS.gene63288.t1 |
| chr3.3 | exon | 60606017 | 60606169 | 60606017 | ID=MS.gene63288.t1.exon14;Parent=MS.gene63288.t1 |
| chr3.3 | CDS | 60606017 | 60606169 | 60606017 | ID=cds.MS.gene63288.t1;Parent=MS.gene63288.t1 |
| chr3.3 | exon | 60605716 | 60605864 | 60605716 | ID=MS.gene63288.t1.exon15;Parent=MS.gene63288.t1 |
| chr3.3 | CDS | 60605716 | 60605864 | 60605716 | ID=cds.MS.gene63288.t1;Parent=MS.gene63288.t1 |
| chr3.3 | exon | 60605408 | 60605455 | 60605408 | ID=MS.gene63288.t1.exon16;Parent=MS.gene63288.t1 |
| chr3.3 | CDS | 60605408 | 60605455 | 60605408 | ID=cds.MS.gene63288.t1;Parent=MS.gene63288.t1 |
| chr3.3 | exon | 60605185 | 60605290 | 60605185 | ID=MS.gene63288.t1.exon17;Parent=MS.gene63288.t1 |
| chr3.3 | CDS | 60605185 | 60605290 | 60605185 | ID=cds.MS.gene63288.t1;Parent=MS.gene63288.t1 |
| chr3.3 | exon | 60603527 | 60603606 | 60603527 | ID=MS.gene63288.t1.exon18;Parent=MS.gene63288.t1 |
| chr3.3 | CDS | 60603527 | 60603606 | 60603527 | ID=cds.MS.gene63288.t1;Parent=MS.gene63288.t1 |
| chr3.3 | exon | 60603357 | 60603396 | 60603357 | ID=MS.gene63288.t1.exon19;Parent=MS.gene63288.t1 |
| chr3.3 | CDS | 60603357 | 60603396 | 60603357 | ID=cds.MS.gene63288.t1;Parent=MS.gene63288.t1 |
| chr3.3 | exon | 60603071 | 60603163 | 60603071 | ID=MS.gene63288.t1.exon20;Parent=MS.gene63288.t1 |
| chr3.3 | CDS | 60603071 | 60603163 | 60603071 | ID=cds.MS.gene63288.t1;Parent=MS.gene63288.t1 |
| chr3.3 | exon | 60602301 | 60602466 | 60602301 | ID=MS.gene63288.t1.exon21;Parent=MS.gene63288.t1 |
| chr3.3 | CDS | 60602301 | 60602466 | 60602301 | ID=cds.MS.gene63288.t1;Parent=MS.gene63288.t1 |
| chr3.3 | exon | 60601153 | 60601426 | 60601153 | ID=MS.gene63288.t1.exon22;Parent=MS.gene63288.t1 |
| chr3.3 | CDS | 60601153 | 60601426 | 60601153 | ID=cds.MS.gene63288.t1;Parent=MS.gene63288.t1 |
| chr3.3 | exon | 60600795 | 60600983 | 60600795 | ID=MS.gene63288.t1.exon23;Parent=MS.gene63288.t1 |
| chr3.3 | CDS | 60600795 | 60600983 | 60600795 | ID=cds.MS.gene63288.t1;Parent=MS.gene63288.t1 |
| Gene Sequence |
| Protein sequence |
