AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene82259

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82259.t1 MTR_4g029440 94.264 401 19 2 1 398 1 400 0.0 744
MS.gene82259.t1 MTR_4g029440 94.162 394 19 2 8 398 2 394 0.0 730
MS.gene82259.t1 MTR_2g061230 43.678 261 117 7 1 260 1 232 1.86e-54 183
MS.gene82259.t1 MTR_7g093390 34.699 366 179 12 55 380 93 438 2.70e-47 170
MS.gene82259.t1 MTR_1g054280 56.044 91 38 2 127 216 71 160 3.23e-29 116
MS.gene82259.t1 MTR_1g054260 48.000 125 43 5 125 232 77 196 5.62e-29 113
MS.gene82259.t1 MTR_1g054465 45.926 135 48 7 125 239 45 174 7.87e-28 110
MS.gene82259.t1 MTR_1g054210 52.427 103 45 3 117 218 109 208 8.21e-28 111
MS.gene82259.t1 MTR_2g090330 46.667 90 44 1 130 215 28 117 1.65e-26 105
MS.gene82259.t1 MTR_2g090330 46.667 90 44 1 130 215 31 120 1.16e-24 100
MS.gene82259.t1 MTR_2g090325 47.727 88 38 2 127 214 15 94 3.73e-23 97.4
MS.gene82259.t1 MTR_5g019300 45.977 87 46 1 132 218 35 120 1.26e-22 97.1
MS.gene82259.t1 MTR_1g021895 45.055 91 48 2 128 218 27 115 1.62e-22 96.7
MS.gene82259.t1 MTR_8g465910 46.392 97 42 3 127 215 41 135 2.64e-21 93.6
MS.gene82259.t1 MTR_2g090295 40.171 117 56 3 105 214 20 129 9.35e-21 92.0
MS.gene82259.t1 MTR_2g013550 39.091 110 50 3 116 213 11 115 2.39e-20 90.5
MS.gene82259.t1 MTR_4g088870 39.423 104 54 2 125 219 25 128 3.74e-20 89.0
MS.gene82259.t1 MTR_4g088875 36.449 107 58 2 123 219 22 128 3.46e-19 87.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82259.t1 AT2G44410 43.462 260 104 8 1 245 10 241 3.31e-53 185
MS.gene82259.t1 AT3G58030 50.365 137 57 6 113 244 114 244 8.15e-35 135
MS.gene82259.t1 AT3G58030 50.365 137 57 6 113 244 114 244 8.15e-35 135
MS.gene82259.t1 AT3G58030 50.365 137 57 6 113 244 114 244 8.15e-35 135
MS.gene82259.t1 AT3G58030 50.365 137 57 6 113 244 114 244 8.15e-35 135
MS.gene82259.t1 AT1G74990 44.860 107 58 1 125 231 10 115 5.55e-29 111
MS.gene82259.t1 AT2G42030 38.272 162 88 3 93 250 100 253 2.52e-28 117
MS.gene82259.t1 AT1G19310 48.276 87 44 1 132 218 21 106 1.09e-23 99.8
MS.gene82259.t1 AT4G28270 46.000 100 34 3 128 214 15 107 1.19e-22 95.9
MS.gene82259.t1 AT2G23780 45.977 87 46 1 132 218 26 111 1.92e-22 96.3
MS.gene82259.t1 AT2G23780 45.977 87 46 1 132 218 26 111 1.92e-22 96.3
MS.gene82259.t1 AT2G23780 45.977 87 46 1 132 218 26 111 1.92e-22 96.3
MS.gene82259.t1 AT4G27470 35.200 125 64 3 132 243 42 162 5.12e-19 86.7
MS.gene82259.t1 AT4G03510 43.956 91 38 4 132 214 46 131 2.74e-18 84.7
MS.gene82259.t1 AT4G03510 43.956 91 38 4 132 214 46 131 2.74e-18 84.7
MS.gene82259.t1 AT4G03510 43.956 91 38 4 132 214 46 131 2.74e-18 84.7
MS.gene82259.t1 AT4G03510 43.956 91 38 4 132 214 46 131 2.74e-18 84.7

Find 115 sgRNAs with CRISPR-Local

Find 172 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGGGTATTATTCCAATTTA+TGG 0.104536 4.2:+74740888 MS.gene82259:CDS
CCATTTCCATGTCCATAAAT+TGG 0.194274 4.2:-74740900 None:intergenic
TTATCAACATTCCTACTTAA+AGG 0.205288 4.2:-74741467 None:intergenic
CTCTCAGGTGTGGTACTATT+AGG 0.243365 4.2:-74741113 None:intergenic
GTCGATAATGTTGCTGTTAT+AGG 0.252556 4.2:+74742985 MS.gene82259:CDS
CATAACCGAATTGGTGGATT+TGG 0.278139 4.2:+74741068 MS.gene82259:CDS
CTTCTGTGTCTCTGTTAAAT+AGG 0.287511 4.2:+74742959 MS.gene82259:CDS
TCTCAGGTGTGGTACTATTA+GGG 0.289538 4.2:-74741112 None:intergenic
GTTGATAATGTTGCTGTTGC+AGG 0.300831 4.2:+74741482 MS.gene82259:CDS
CTTCTATTGTCCAAAGTATT+CGG 0.320027 4.2:+74741039 MS.gene82259:CDS
CTTTGATTGTAATATTTGTT+TGG 0.349272 4.2:+74740740 MS.gene82259:CDS
TGATTTGAATCAGGAGCCTT+TGG 0.350787 4.2:+74740356 MS.gene82259:CDS
TATGAAACCGCCGAATACTT+TGG 0.373232 4.2:-74741049 None:intergenic
TTCGAGAGCGAGGCAGCGTC+GGG 0.374026 4.2:+74740485 MS.gene82259:CDS
ATTCAGATAACCAATGTTAC+AGG 0.375175 4.2:+74740531 MS.gene82259:CDS
TTCTGCTGAAAGATTAGTTC+AGG 0.376998 4.2:+74741274 MS.gene82259:CDS
CGGCGGTTTCATAACCGAAT+TGG 0.383576 4.2:+74741059 MS.gene82259:CDS
CCAATTTATGGACATGGAAA+TGG 0.384021 4.2:+74740900 MS.gene82259:CDS
ACATTCCTTTGCTTTCGAAT+AGG 0.388726 4.2:-74740838 None:intergenic
TATGGAGCTTGATTTGAATC+AGG 0.400185 4.2:+74740347 None:intergenic
CTCTCGAAGTAATTGCTTCA+AGG 0.402049 4.2:-74740470 None:intergenic
GGAGCGGAACGATGCTGATC+AGG 0.409682 4.2:+74740599 MS.gene82259:CDS
GACCATTGATATATGATTCA+AGG 0.415979 4.2:-74741298 None:intergenic
TGAACATGGTGGTTCTCAAC+AGG 0.416867 4.2:+74741181 MS.gene82259:CDS
AGCTGCTGCAGCTACAGAAT+TGG 0.416919 4.2:-74741435 None:intergenic
TGCAGTTTCTCCTGTAACAT+TGG 0.419434 4.2:-74740541 None:intergenic
CTTCTCTTTCTCCTTTAAGT+AGG 0.422331 4.2:+74741456 MS.gene82259:CDS
CTTCTTCTACCGTTTGATGA+AGG 0.424965 4.2:-74743057 None:intergenic
GTGGTTCTCAACAGGTTTCA+AGG 0.428291 4.2:+74741189 MS.gene82259:CDS
ATCTGAATCCGAGAGGAGTT+CGG 0.434023 4.2:-74740516 None:intergenic
GTTACCTCCCCTTTACAAAC+AGG 0.438464 4.2:-74740861 None:intergenic
TTGCTTCAAGGTGTGTGATT+CGG 0.441946 4.2:-74740458 None:intergenic
GTCTGCGTGCGAGACTGAAC+AGG 0.444476 4.2:-74741149 None:intergenic
GGGATGGAAGAGATTGATAA+TGG 0.448635 4.2:+74740690 MS.gene82259:CDS
AGGAATGTCCTGTTTGTAAA+GGG 0.451056 4.2:+74740853 MS.gene82259:CDS
AAATGAACGCACTGATGTAC+TGG 0.451580 4.2:-74740388 None:intergenic
AGTCATTTCGATATTCTTGT+TGG 0.454214 4.2:-74743111 None:intergenic
TGACCACAACATGTCAAAAC+AGG 0.454953 4.2:-74740777 None:intergenic
TATATCAATGGTCACAATAC+AGG 0.455204 4.2:+74741308 MS.gene82259:CDS
TAGAAGAAGAAGATGCTCCT+TGG 0.455800 4.2:-74741022 None:intergenic
TAAAGGGGAGGTAACTGAAT+CGG 0.458742 4.2:+74740869 MS.gene82259:CDS
GGCAAACCATTGTTTCTCTC+AGG 0.458959 4.2:-74741128 None:intergenic
TTCTTCTACCGTTTGATGAA+GGG 0.463767 4.2:-74743056 None:intergenic
CTCTCAACTCTAGGTGCCTT+AGG 0.467180 4.2:-74740978 None:intergenic
TATGTTGGAAGAACTTGAAT+CGG 0.467329 4.2:+74740416 MS.gene82259:CDS
AGAAGAAGAAGATGCTCCTT+GGG 0.474601 4.2:-74741021 None:intergenic
GGTAAAGTGGAGGAGAGTAG+TGG 0.474653 4.2:+74740711 MS.gene82259:CDS
CGGTTTCATAACCGAATTGG+TGG 0.476371 4.2:+74741062 MS.gene82259:CDS
GCTCTCCAAATCCACCAATT+CGG 0.478712 4.2:-74741073 None:intergenic
AACGATGCTGATCAGGATGA+AGG 0.485394 4.2:+74740606 MS.gene82259:CDS
TCAAACGGTAGAAGAAGAAA+TGG 0.486110 4.2:+74743063 MS.gene82259:CDS
GGTTGCCGCCGAACTCCTCT+CGG 0.492462 4.2:+74740508 MS.gene82259:CDS
CTTGACCACGACTCTCTGAC+TGG 0.492720 4.2:-74741397 None:intergenic
AGAGATTGATAATGGTAAAG+TGG 0.493881 4.2:+74740698 MS.gene82259:CDS
CATTTGATAGCAAAAGCTCT+AGG 0.498838 4.2:+74740669 MS.gene82259:CDS
GAGAGTTCCTCCTCGACCTA+AGG 0.502667 4.2:+74740962 MS.gene82259:CDS
AAGGAATGTCCTGTTTGTAA+AGG 0.503450 4.2:+74740852 MS.gene82259:CDS
GGATAGTTGAAAGCGTGAAA+GGG 0.503778 4.2:+74740628 MS.gene82259:CDS
AACAGCATTAAATTCTGCAA+TGG 0.508273 4.2:+74741250 MS.gene82259:CDS
CTTCGAGAGCGAGGCAGCGT+CGG 0.509799 4.2:+74740484 MS.gene82259:CDS
GAAGAAGAAGATTGCGATGA+CGG 0.511269 4.2:+74741618 MS.gene82259:CDS
CTGTTAGACCCTTCATCAAA+CGG 0.512392 4.2:+74743048 MS.gene82259:CDS
GACCTTGAATCATATATCAA+TGG 0.517570 4.2:+74741296 MS.gene82259:CDS
AAAGGGGAGGTAACTGAATC+GGG 0.517863 4.2:+74740870 MS.gene82259:CDS
GGGATAGTTGAAAGCGTGAA+AGG 0.520570 4.2:+74740627 MS.gene82259:CDS
GCGAAGAACGGAGGGAGGAG+CGG 0.521333 4.2:+74740583 MS.gene82259:CDS
ATTATTCCAATTTATGGACA+TGG 0.526065 4.2:+74740894 MS.gene82259:CDS
GTAAAGTGGAGGAGAGTAGT+GGG 0.535109 4.2:+74740712 MS.gene82259:CDS
TACAACTGATAGAAATGTAC+AGG 0.535539 4.2:+74741517 MS.gene82259:CDS
GCAACAGCAGGTGATGTTCA+AGG 0.537071 4.2:+74740561 MS.gene82259:CDS
TCGAGGAGGAACTCTCAAAC+CGG 0.538001 4.2:-74740955 None:intergenic
ACTCTAGGTGCCTTAGGTCG+AGG 0.546117 4.2:-74740972 None:intergenic
CTGATGGAAATGAAAGAAGC+CGG 0.547924 4.2:+74740936 MS.gene82259:CDS
GTTGAATTTCTAGTAACAGT+AGG 0.548372 4.2:-74741353 None:intergenic
GAATTTCTAGTAACAGTAGG+AGG 0.555492 4.2:-74741350 None:intergenic
TGTCTAATACTCTCAACTCT+AGG 0.555708 4.2:-74740987 None:intergenic
GATAGTTGAAAGCGTGAAAG+GGG 0.559865 4.2:+74740629 MS.gene82259:CDS
GTTTCAAGGTTGCTAGAGCA+AGG 0.560862 4.2:+74741203 MS.gene82259:CDS
GCGTTCATTTGATTCTATGT+TGG 0.571256 4.2:+74740401 MS.gene82259:CDS
ACAGGAGAAACTGCAACAGC+AGG 0.572374 4.2:+74740549 MS.gene82259:CDS
GTGATGTTCAAGGCGAAGAA+CGG 0.575661 4.2:+74740571 MS.gene82259:CDS
CGAGAGCGAGGCAGCGTCGG+GGG 0.578008 4.2:+74740487 MS.gene82259:CDS
TGGCGGCGGTGATTGTCTGA+TGG 0.578360 4.2:+74740920 MS.gene82259:CDS
ATTTGATAGCAAAAGCTCTA+GGG 0.584132 4.2:+74740670 MS.gene82259:CDS
TAGTTGAAAGCGTGAAAGGG+GGG 0.585256 4.2:+74740631 MS.gene82259:CDS
ATAGTTGAAAGCGTGAAAGG+GGG 0.588095 4.2:+74740630 MS.gene82259:CDS
GATTGATAATGGTAAAGTGG+AGG 0.594410 4.2:+74740701 MS.gene82259:CDS
ACCATTGTTTCTCTCAGGTG+TGG 0.595758 4.2:-74741123 None:intergenic
ACGATGCTGATCAGGATGAA+GGG 0.597581 4.2:+74740607 MS.gene82259:CDS
TATGGACATGGAAATGGCGG+CGG 0.603447 4.2:+74740906 MS.gene82259:CDS
CAGACAGACAACAATGAACA+TGG 0.607844 4.2:+74741167 MS.gene82259:CDS
GATAGCAAAAGCTCTAGGGA+TGG 0.616270 4.2:+74740674 MS.gene82259:CDS
ATGTTCAAGGCGAAGAACGG+AGG 0.619140 4.2:+74740574 MS.gene82259:CDS
TCTTCATCTAGAGGTAGAAG+AGG 0.623511 4.2:+74741557 MS.gene82259:intron
AGACAGAAGTTGCTAACCCA+AGG 0.623915 4.2:+74741005 MS.gene82259:CDS
TCGAGAGCGAGGCAGCGTCG+GGG 0.626763 4.2:+74740486 MS.gene82259:CDS
TGTTCAAGGCGAAGAACGGA+GGG 0.630328 4.2:+74740575 MS.gene82259:CDS
AAGCAATTACTTCGAGAGCG+AGG 0.636181 4.2:+74740475 MS.gene82259:CDS
TGCAGCTGTAGAATCAGTCA+CGG 0.640246 4.2:-74742932 None:intergenic
ACCACACCTGAGAGAAACAA+TGG 0.640564 4.2:+74741122 MS.gene82259:CDS
ATCAATGGTCACAATACAGG+AGG 0.640899 4.2:+74741311 MS.gene82259:CDS
ATGTCCTGTTTGTAAAGGGG+AGG 0.641102 4.2:+74740857 MS.gene82259:CDS
GTATGCCTATTCGAAAGCAA+AGG 0.642527 4.2:+74740833 MS.gene82259:CDS
ATTTATGGACATGGAAATGG+CGG 0.647005 4.2:+74740903 MS.gene82259:CDS
GGAATGTCCTGTTTGTAAAG+GGG 0.658869 4.2:+74740854 MS.gene82259:CDS
CTATTGTCCAAAGTATTCGG+CGG 0.665724 4.2:+74741042 MS.gene82259:CDS
GTACTGGTTTGATGATCCAA+AGG 0.668438 4.2:-74740372 None:intergenic
TCAAGGCGAAGAACGGAGGG+AGG 0.671112 4.2:+74740578 MS.gene82259:CDS
AGGCTCAGAAATTCAGACAA+CGG 0.673554 4.2:+74743005 MS.gene82259:CDS
CTAGGTGCCTTAGGTCGAGG+AGG 0.679868 4.2:-74740969 None:intergenic
TACGATAACTGATAGAAACA+CGG 0.689120 4.2:-74740813 None:intergenic
ACAAACCAGTCAGAGAGTCG+TGG 0.695607 4.2:+74741392 MS.gene82259:CDS
TGAATCCGAGAGGAGTTCGG+CGG 0.720440 4.2:-74740513 None:intergenic
ATTGGTTATCTGAATCCGAG+AGG 0.732391 4.2:-74740523 None:intergenic
ACAGACAACAATGAACATGG+TGG 0.773237 4.2:+74741170 MS.gene82259:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGAAAATTAATTTAAAATGA+AGG + chr4.2:74742737-74742756 MS.gene82259:intron 10.0%
!! AACATATTTGATATTTGTCT+TGG - chr4.2:74741743-74741762 None:intergenic 20.0%
!! ATCTAATAAAGAACATGTAA+AGG - chr4.2:74742160-74742179 None:intergenic 20.0%
!! CTTTGATTGTAATATTTGTT+TGG + chr4.2:74740740-74740759 MS.gene82259:CDS 20.0%
!! TCTAATAAAGAACATGTAAA+GGG - chr4.2:74742159-74742178 None:intergenic 20.0%
!! TTAAACTGTTTATCCAAATA+GGG + chr4.2:74742049-74742068 MS.gene82259:intron 20.0%
!!! AGTTTGTTGTATATATCATA+TGG - chr4.2:74742450-74742469 None:intergenic 20.0%
!!! AGTTTTATTCAATAGATGTA+AGG + chr4.2:74741790-74741809 MS.gene82259:intron 20.0%
!!! TATCATGATTGTTTCTTATA+AGG + chr4.2:74742392-74742411 MS.gene82259:intron 20.0%
!!! TCTTTTGAATATCTAGTTTT+TGG + chr4.2:74742189-74742208 MS.gene82259:intron 20.0%
! ACTCTAATGTTAAGCTAATT+AGG - chr4.2:74742550-74742569 None:intergenic 25.0%
! ATTATTCCAATTTATGGACA+TGG + chr4.2:74740894-74740913 MS.gene82259:CDS 25.0%
! CAAAAAAAGATAGAGACTTA+TGG + chr4.2:74742852-74742871 MS.gene82259:intron 25.0%
! GTTAAACTGTTTATCCAAAT+AGG + chr4.2:74742048-74742067 MS.gene82259:intron 25.0%
! GTTCTTTATTAGATCTATCT+TGG + chr4.2:74742166-74742185 MS.gene82259:intron 25.0%
! TTATCAACATTCCTACTTAA+AGG - chr4.2:74741470-74741489 None:intergenic 25.0%
!! ACTTAAGTTTTCTGTCTTTA+TGG - chr4.2:74742713-74742732 None:intergenic 25.0%
!! ACTTATTTGAGATTATCTAC+TGG + chr4.2:74741833-74741852 MS.gene82259:intron 25.0%
!! CATAAACTATAGAGTATTAG+AGG - chr4.2:74742318-74742337 None:intergenic 25.0%
!!! TATCTAGTTTTTGGATCTTT+TGG + chr4.2:74742198-74742217 MS.gene82259:intron 25.0%
AACAGCATTAAATTCTGCAA+TGG + chr4.2:74741250-74741269 MS.gene82259:CDS 30.0%
ATTCAGATAACCAATGTTAC+AGG + chr4.2:74740531-74740550 MS.gene82259:CDS 30.0%
CATACTGTAACAAACATAAG+AGG - chr4.2:74742093-74742112 None:intergenic 30.0%
CATGCAACATGTTGTATTAT+CGG + chr4.2:74742360-74742379 MS.gene82259:intron 30.0%
GACCATTGATATATGATTCA+AGG - chr4.2:74741301-74741320 None:intergenic 30.0%
GACCTTGAATCATATATCAA+TGG + chr4.2:74741296-74741315 MS.gene82259:CDS 30.0%
GATTACTTGAATACAACCTT+TGG + chr4.2:74742285-74742304 MS.gene82259:intron 30.0%
TACAACTGATAGAAATGTAC+AGG + chr4.2:74741517-74741536 MS.gene82259:CDS 30.0%
TACGATAACTGATAGAAACA+CGG - chr4.2:74740816-74740835 None:intergenic 30.0%
TATATCAATGGTCACAATAC+AGG + chr4.2:74741308-74741327 MS.gene82259:CDS 30.0%
TATGTTGGAAGAACTTGAAT+CGG + chr4.2:74740416-74740435 MS.gene82259:CDS 30.0%
TCAAATAAGTCAATCCAAAC+AGG - chr4.2:74741823-74741842 None:intergenic 30.0%
TCGGGTATTATTCCAATTTA+TGG + chr4.2:74740888-74740907 MS.gene82259:CDS 30.0%
! ATTTGATAGCAAAAGCTCTA+GGG + chr4.2:74740670-74740689 MS.gene82259:CDS 30.0%
! CTCTTATGTTTGTTACAGTA+TGG + chr4.2:74742091-74742110 MS.gene82259:intron 30.0%
! CTTCTATTGTCCAAAGTATT+CGG + chr4.2:74741039-74741058 MS.gene82259:CDS 30.0%
! TTTTGGTCAGTATTCAGATT+GGG + chr4.2:74742215-74742234 MS.gene82259:intron 30.0%
!! AGAGATTGATAATGGTAAAG+TGG + chr4.2:74740698-74740717 MS.gene82259:CDS 30.0%
!! GTTGAATTTCTAGTAACAGT+AGG - chr4.2:74741356-74741375 None:intergenic 30.0%
AAGGAATGTCCTGTTTGTAA+AGG + chr4.2:74740852-74740871 MS.gene82259:CDS 35.0%
ACATTCCTTTGCTTTCGAAT+AGG - chr4.2:74740841-74740860 None:intergenic 35.0%
AGAGGATATTGACAATTAGC+AGG - chr4.2:74742075-74742094 None:intergenic 35.0%
AGGAATGTCCTGTTTGTAAA+GGG + chr4.2:74740853-74740872 MS.gene82259:CDS 35.0%
ATCCTGTTGTTGCATGTAAT+AGG + chr4.2:74742886-74742905 MS.gene82259:intron 35.0%
ATTTATGGACATGGAAATGG+CGG + chr4.2:74740903-74740922 MS.gene82259:CDS 35.0%
CCAATTTATGGACATGGAAA+TGG + chr4.2:74740900-74740919 MS.gene82259:CDS 35.0%
CCATTTCCATGTCCATAAAT+TGG - chr4.2:74740903-74740922 None:intergenic 35.0%
CTTCTCTTTCTCCTTTAAGT+AGG + chr4.2:74741456-74741475 MS.gene82259:CDS 35.0%
CTTCTGTGTCTCTGTTAAAT+AGG + chr4.2:74742959-74742978 MS.gene82259:CDS 35.0%
GCAATGCACATGATCTTTAT+GGG - chr4.2:74742341-74742360 None:intergenic 35.0%
GCGTTCATTTGATTCTATGT+TGG + chr4.2:74740401-74740420 MS.gene82259:CDS 35.0%
TATTAGAGGCTCTTATCCAA+AGG - chr4.2:74742304-74742323 None:intergenic 35.0%
TATTTCCACTCCTTCTGTAT+TGG + chr4.2:74742604-74742623 MS.gene82259:intron 35.0%
TCAAACGGTAGAAGAAGAAA+TGG + chr4.2:74743063-74743082 MS.gene82259:CDS 35.0%
TCCTGTTGTTGCATGTAATA+GGG + chr4.2:74742887-74742906 MS.gene82259:intron 35.0%
TGCAATGCACATGATCTTTA+TGG - chr4.2:74742342-74742361 None:intergenic 35.0%
TGTAAGGATATTTGCCTGTT+TGG + chr4.2:74741806-74741825 MS.gene82259:intron 35.0%
TGTCTAATACTCTCAACTCT+AGG - chr4.2:74740990-74741009 None:intergenic 35.0%
TTCTACCTCTAGATGAAGAA+GGG - chr4.2:74741556-74741575 None:intergenic 35.0%
TTCTTCTACCGTTTGATGAA+GGG - chr4.2:74743059-74743078 None:intergenic 35.0%
! ATGACGGTCTTTTATGTAAG+TGG + chr4.2:74741634-74741653 MS.gene82259:intron 35.0%
! CATTTGATAGCAAAAGCTCT+AGG + chr4.2:74740669-74740688 MS.gene82259:CDS 35.0%
! CTTTTGGTCAGTATTCAGAT+TGG + chr4.2:74742214-74742233 MS.gene82259:intron 35.0%
! GTCGATAATGTTGCTGTTAT+AGG + chr4.2:74742985-74743004 MS.gene82259:CDS 35.0%
! TTCTGCTGAAAGATTAGTTC+AGG + chr4.2:74741274-74741293 MS.gene82259:CDS 35.0%
!! GAATTTCTAGTAACAGTAGG+AGG - chr4.2:74741353-74741372 None:intergenic 35.0%
!! GATTGATAATGGTAAAGTGG+AGG + chr4.2:74740701-74740720 MS.gene82259:CDS 35.0%
!!! GTTGTGGTCATTTGTTTTGT+TGG + chr4.2:74740790-74740809 MS.gene82259:CDS 35.0%
ACAGACAACAATGAACATGG+TGG + chr4.2:74741170-74741189 MS.gene82259:CDS 40.0%
ACATGCAACAACAGGATATC+TGG - chr4.2:74742883-74742902 None:intergenic 40.0%
AGAAGAAGAAGATGCTCCTT+GGG - chr4.2:74741024-74741043 None:intergenic 40.0%
AGGCTCAGAAATTCAGACAA+CGG + chr4.2:74743005-74743024 MS.gene82259:CDS 40.0%
AGTATGCATGCATGTAAACG+AGG - chr4.2:74741685-74741704 None:intergenic 40.0%
ATCAATGGTCACAATACAGG+AGG + chr4.2:74741311-74741330 MS.gene82259:CDS 40.0%
ATTGGTTATCTGAATCCGAG+AGG - chr4.2:74740526-74740545 None:intergenic 40.0%
CAGACAGACAACAATGAACA+TGG + chr4.2:74741167-74741186 MS.gene82259:CDS 40.0%
CGCAATCTTCTTCTTCTTCT+TGG - chr4.2:74741614-74741633 None:intergenic 40.0%
CTCTCGAAGTAATTGCTTCA+AGG - chr4.2:74740473-74740492 None:intergenic 40.0%
CTGATGGAAATGAAAGAAGC+CGG + chr4.2:74740936-74740955 MS.gene82259:CDS 40.0%
CTGTTAGACCCTTCATCAAA+CGG + chr4.2:74743048-74743067 MS.gene82259:CDS 40.0%
CTTCTACCTCTAGATGAAGA+AGG - chr4.2:74741557-74741576 None:intergenic 40.0%
CTTCTTCTACCGTTTGATGA+AGG - chr4.2:74743060-74743079 None:intergenic 40.0%
GAAGAAGAAGATTGCGATGA+CGG + chr4.2:74741618-74741637 MS.gene82259:CDS 40.0%
GGAATGTCCTGTTTGTAAAG+GGG + chr4.2:74740854-74740873 MS.gene82259:CDS 40.0%
GGGATGGAAGAGATTGATAA+TGG + chr4.2:74740690-74740709 MS.gene82259:CDS 40.0%
GTATGCCTATTCGAAAGCAA+AGG + chr4.2:74740833-74740852 MS.gene82259:CDS 40.0%
TAAAGGGGAGGTAACTGAAT+CGG + chr4.2:74740869-74740888 MS.gene82259:CDS 40.0%
TAGAAGAAGAAGATGCTCCT+TGG - chr4.2:74741025-74741044 None:intergenic 40.0%
TATGAAACCGCCGAATACTT+TGG - chr4.2:74741052-74741071 None:intergenic 40.0%
TCCCTATTACATGCAACAAC+AGG - chr4.2:74742891-74742910 None:intergenic 40.0%
TCTCAGGTGTGGTACTATTA+GGG - chr4.2:74741115-74741134 None:intergenic 40.0%
TCTTCATCTAGAGGTAGAAG+AGG + chr4.2:74741557-74741576 MS.gene82259:intron 40.0%
TGACCACAACATGTCAAAAC+AGG - chr4.2:74740780-74740799 None:intergenic 40.0%
TGATTTGAATCAGGAGCCTT+TGG + chr4.2:74740356-74740375 MS.gene82259:CDS 40.0%
TGCAGTTTCTCCTGTAACAT+TGG - chr4.2:74740544-74740563 None:intergenic 40.0%
TGTCTCACTTGAGTAAGTTC+AGG + chr4.2:74742114-74742133 MS.gene82259:intron 40.0%
TTGCTTCAAGGTGTGTGATT+CGG - chr4.2:74740461-74740480 None:intergenic 40.0%
! AAATGAACGCACTGATGTAC+TGG - chr4.2:74740391-74740410 None:intergenic 40.0%
! ATAGTTGAAAGCGTGAAAGG+GGG + chr4.2:74740630-74740649 MS.gene82259:CDS 40.0%
! CAACAAACTAGAAGCATGTG+TGG + chr4.2:74742461-74742480 MS.gene82259:intron 40.0%
! CTATTGTCCAAAGTATTCGG+CGG + chr4.2:74741042-74741061 MS.gene82259:CDS 40.0%
! GATAGTTGAAAGCGTGAAAG+GGG + chr4.2:74740629-74740648 MS.gene82259:CDS 40.0%
! GGATAGTTGAAAGCGTGAAA+GGG + chr4.2:74740628-74740647 MS.gene82259:CDS 40.0%
!! CATAACCGAATTGGTGGATT+TGG + chr4.2:74741068-74741087 MS.gene82259:CDS 40.0%
!! GTACTGGTTTGATGATCCAA+AGG - chr4.2:74740375-74740394 None:intergenic 40.0%
!! GTTGATAATGTTGCTGTTGC+AGG + chr4.2:74741482-74741501 MS.gene82259:CDS 40.0%
!!! GATCCTGTTTTGACATGTTG+TGG + chr4.2:74740774-74740793 MS.gene82259:CDS 40.0%
AAAGGGGAGGTAACTGAATC+GGG + chr4.2:74740870-74740889 MS.gene82259:CDS 45.0%
AACGATGCTGATCAGGATGA+AGG + chr4.2:74740606-74740625 MS.gene82259:CDS 45.0%
AAGCAATTACTTCGAGAGCG+AGG + chr4.2:74740475-74740494 MS.gene82259:CDS 45.0%
ACCACAAACTGGAGTCAATG+AGG - chr4.2:74742249-74742268 None:intergenic 45.0%
ACCACACCTGAGAGAAACAA+TGG + chr4.2:74741122-74741141 MS.gene82259:CDS 45.0%
ACGATGCTGATCAGGATGAA+GGG + chr4.2:74740607-74740626 MS.gene82259:CDS 45.0%
AGACAGAAGTTGCTAACCCA+AGG + chr4.2:74741005-74741024 MS.gene82259:CDS 45.0%
ATCGTCATACGAGTTGCTGT+CGG - chr4.2:74742637-74742656 None:intergenic 45.0%
ATCTGAATCCGAGAGGAGTT+CGG - chr4.2:74740519-74740538 None:intergenic 45.0%
ATGTCCTGTTTGTAAAGGGG+AGG + chr4.2:74740857-74740876 MS.gene82259:CDS 45.0%
CGGTTTCATAACCGAATTGG+TGG + chr4.2:74741062-74741081 MS.gene82259:CDS 45.0%
CTAGACCCTTCTTCATCTAG+AGG + chr4.2:74741548-74741567 MS.gene82259:intron 45.0%
CTCTCAGGTGTGGTACTATT+AGG - chr4.2:74741116-74741135 None:intergenic 45.0%
GACAATTAGCAGGCCCTATT+TGG - chr4.2:74742065-74742084 None:intergenic 45.0%
GCTCTCCAAATCCACCAATT+CGG - chr4.2:74741076-74741095 None:intergenic 45.0%
GTAAAGTGGAGGAGAGTAGT+GGG + chr4.2:74740712-74740731 MS.gene82259:CDS 45.0%
GTGATGTTCAAGGCGAAGAA+CGG + chr4.2:74740571-74740590 MS.gene82259:CDS 45.0%
GTGGTTCTCAACAGGTTTCA+AGG + chr4.2:74741189-74741208 MS.gene82259:CDS 45.0%
GTTACCTCCCCTTTACAAAC+AGG - chr4.2:74740864-74740883 None:intergenic 45.0%
GTTTCAAGGTTGCTAGAGCA+AGG + chr4.2:74741203-74741222 MS.gene82259:CDS 45.0%
TGAACATGGTGGTTCTCAAC+AGG + chr4.2:74741181-74741200 MS.gene82259:CDS 45.0%
TGCAGCTGTAGAATCAGTCA+CGG - chr4.2:74742935-74742954 None:intergenic 45.0%
TTGTGCCAATACAGAAGGAG+TGG - chr4.2:74742612-74742631 None:intergenic 45.0%
! ACCATTGTTTCTCTCAGGTG+TGG - chr4.2:74741126-74741145 None:intergenic 45.0%
! ACTCCTTCTGTATTGGCACA+AGG + chr4.2:74742611-74742630 MS.gene82259:intron 45.0%
! GGCAAACCATTGTTTCTCTC+AGG - chr4.2:74741131-74741150 None:intergenic 45.0%
! GGGATAGTTGAAAGCGTGAA+AGG + chr4.2:74740627-74740646 MS.gene82259:CDS 45.0%
! TAGTTGAAAGCGTGAAAGGG+GGG + chr4.2:74740631-74740650 MS.gene82259:CDS 45.0%
!! GATAGCAAAAGCTCTAGGGA+TGG + chr4.2:74740674-74740693 MS.gene82259:CDS 45.0%
!! TCATTGACTCCAGTTTGTGG+TGG + chr4.2:74742248-74742267 MS.gene82259:intron 45.0%
AAAGCGTGAAAGGGGGGAAA+AGG + chr4.2:74740637-74740656 MS.gene82259:CDS 50.0%
ACAAACCAGTCAGAGAGTCG+TGG + chr4.2:74741392-74741411 MS.gene82259:CDS 50.0%
ACAGGAGAAACTGCAACAGC+AGG + chr4.2:74740549-74740568 MS.gene82259:CDS 50.0%
AGCTGCTGCAGCTACAGAAT+TGG - chr4.2:74741438-74741457 None:intergenic 50.0%
ATGTTCAAGGCGAAGAACGG+AGG + chr4.2:74740574-74740593 MS.gene82259:CDS 50.0%
CAAACTGGAGTCAATGAGGC+AGG - chr4.2:74742245-74742264 None:intergenic 50.0%
CGGCGGTTTCATAACCGAAT+TGG + chr4.2:74741059-74741078 MS.gene82259:CDS 50.0%
CTCTCAACTCTAGGTGCCTT+AGG - chr4.2:74740981-74741000 None:intergenic 50.0%
GCAACAGCAGGTGATGTTCA+AGG + chr4.2:74740561-74740580 MS.gene82259:CDS 50.0%
GGTAAAGTGGAGGAGAGTAG+TGG + chr4.2:74740711-74740730 MS.gene82259:CDS 50.0%
TATGGACATGGAAATGGCGG+CGG + chr4.2:74740906-74740925 MS.gene82259:CDS 50.0%
TCGAGGAGGAACTCTCAAAC+CGG - chr4.2:74740958-74740977 None:intergenic 50.0%
TGACTCCAGTTTGTGGTGGT+GGG + chr4.2:74742252-74742271 MS.gene82259:intron 50.0%
TGTTCAAGGCGAAGAACGGA+GGG + chr4.2:74740575-74740594 MS.gene82259:CDS 50.0%
TTGACTCCAGTTTGTGGTGG+TGG + chr4.2:74742251-74742270 MS.gene82259:intron 50.0%
!! GCCTCATTGACTCCAGTTTG+TGG + chr4.2:74742245-74742264 MS.gene82259:intron 50.0%
ACTCTAGGTGCCTTAGGTCG+AGG - chr4.2:74740975-74740994 None:intergenic 55.0%
ATGACCCCACCACCACAAAC+TGG - chr4.2:74742260-74742279 None:intergenic 55.0%
CTTGACCACGACTCTCTGAC+TGG - chr4.2:74741400-74741419 None:intergenic 55.0%
GAGAGTTCCTCCTCGACCTA+AGG + chr4.2:74740962-74740981 MS.gene82259:CDS 55.0%
TGAATCCGAGAGGAGTTCGG+CGG - chr4.2:74740516-74740535 None:intergenic 55.0%
! CGGCCTTGTGCCAATACAGA+AGG - chr4.2:74742617-74742636 None:intergenic 55.0%
! GACTCCAGTTTGTGGTGGTG+GGG + chr4.2:74742253-74742272 MS.gene82259:intron 55.0%
!! AGTTTGTGGTGGTGGGGTCA+TGG + chr4.2:74742259-74742278 MS.gene82259:intron 55.0%
CTAGGTGCCTTAGGTCGAGG+AGG - chr4.2:74740972-74740991 None:intergenic 60.0%
GGAGCGGAACGATGCTGATC+AGG + chr4.2:74740599-74740618 MS.gene82259:CDS 60.0%
GTCTGCGTGCGAGACTGAAC+AGG - chr4.2:74741152-74741171 None:intergenic 60.0%
TCAAGGCGAAGAACGGAGGG+AGG + chr4.2:74740578-74740597 MS.gene82259:CDS 60.0%
TGGCGGCGGTGATTGTCTGA+TGG + chr4.2:74740920-74740939 MS.gene82259:CDS 60.0%
CTTCGAGAGCGAGGCAGCGT+CGG + chr4.2:74740484-74740503 MS.gene82259:CDS 65.0%
GCGAAGAACGGAGGGAGGAG+CGG + chr4.2:74740583-74740602 MS.gene82259:CDS 65.0%
GGTTGCCGCCGAACTCCTCT+CGG + chr4.2:74740508-74740527 MS.gene82259:CDS 65.0%
TTCGAGAGCGAGGCAGCGTC+GGG + chr4.2:74740485-74740504 MS.gene82259:CDS 65.0%
TCGAGAGCGAGGCAGCGTCG+GGG + chr4.2:74740486-74740505 MS.gene82259:CDS 70.0%
! CGAGAGCGAGGCAGCGTCGG+GGG + chr4.2:74740487-74740506 MS.gene82259:CDS 75.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 74740348 74743131 74740348 ID=MS.gene82259
chr4.2 mRNA 74740348 74743131 74740348 ID=MS.gene82259.t1;Parent=MS.gene82259
chr4.2 exon 74740348 74741538 74740348 ID=MS.gene82259.t1.exon1;Parent=MS.gene82259.t1
chr4.2 CDS 74740348 74741538 74740348 ID=cds.MS.gene82259.t1;Parent=MS.gene82259.t1
chr4.2 exon 74741570 74741648 74741570 ID=MS.gene82259.t1.exon2;Parent=MS.gene82259.t1
chr4.2 CDS 74741570 74741648 74741570 ID=cds.MS.gene82259.t1;Parent=MS.gene82259.t1
chr4.2 exon 74742908 74743131 74742908 ID=MS.gene82259.t1.exon3;Parent=MS.gene82259.t1
chr4.2 CDS 74742908 74743131 74742908 ID=cds.MS.gene82259.t1;Parent=MS.gene82259.t1
Gene Sequence

>MS.gene82259

ATGGAGCTTGATTTGAATCAGGAGCCTTTGGATCATCAAACCAGTACATCAGTGCGTTCATTTGATTCTATGTTGGAAGAACTTGAATCGGCTGAAGAAAATATACGAAACCGAATCACACACCTTGAAGCAATTACTTCGAGAGCGAGGCAGCGTCGGGGGTTGCCGCCGAACTCCTCTCGGATTCAGATAACCAATGTTACAGGAGAAACTGCAACAGCAGGTGATGTTCAAGGCGAAGAACGGAGGGAGGAGCGGAACGATGCTGATCAGGATGAAGGGATAGTTGAAAGCGTGAAAGGGGGGAAAAGGAAAAGTTCGCATTTGATAGCAAAAGCTCTAGGGATGGAAGAGATTGATAATGGTAAAGTGGAGGAGAGTAGTGGGAATTTCTTTGATTGTAATATTTGTTTGGATATTGCAAGAGATCCTGTTTTGACATGTTGTGGTCATTTGTTTTGTTGGCCGTGTTTCTATCAGTTATCGTATGCCTATTCGAAAGCAAAGGAATGTCCTGTTTGTAAAGGGGAGGTAACTGAATCGGGTATTATTCCAATTTATGGACATGGAAATGGCGGCGGTGATTGTCTGATGGAAATGAAAGAAGCCGGTTTGAGAGTTCCTCCTCGACCTAAGGCACCTAGAGTTGAGAGTATTAGACAGAAGTTGCTAACCCAAGGAGCATCTTCTTCTTCTATTGTCCAAAGTATTCGGCGGTTTCATAACCGAATTGGTGGATTTGGAGAGCAAGTTCAGTTAGAAAGCCCTAATAGTACCACACCTGAGAGAAACAATGGTTTGCCTGTTCAGTCTCGCACGCAGACAGACAACAATGAACATGGTGGTTCTCAACAGGTTTCAAGGTTGCTAGAGCAAGGAGCTTCTTCGTTTTCGTCACTTTCAACAGCATTAAATTCTGCAATGGATTCTGCTGAAAGATTAGTTCAGGACCTTGAATCATATATCAATGGTCACAATACAGGAGGAAGCAGAGAGCAAAATCCTCCTACTGTTACTAGAAATTCAACTTTAAGTGTCGCTGCTACAAACCAGTCAGAGAGTCGTGGTCAAGATGTTGTTGATCTGACCAATTCTGTAGCTGCAGCAGCTTCTCTTTCTCCTTTAAGTAGGAATGTTGATAATGTTGCTGTTGCAGGCTCACAAATTCATACAACTGATAGAAATGTACAGGTTAGTTTGCTAGACCCTTCTTCATCTAGAGGTAGAAGAGGAAATCGCGCTTCAAGACAAGTTTCAAATGAACCAAGAAGAAGAAGAAGATTGCGATGACGGTCTTTTATGTAAGTGGAAATGCGTTATATTCATCACATGTTCCTCGTTTACATGCATGCATACTACAATTTGTTTGTCATACACAATTTTTATTCTTGTCCAAGACAAATATCAAATATGTTATCTTAGTTATGTACATAAATATATCAAGTTTTATTCAATAGATGTAAGGATATTTGCCTGTTTGGATTGACTTATTTGAGATTATCTACTGGTACAAGAACTTGTGAGAATGTTTGATATTGCTTGTGAAAAAAACTTATGACATGTTCGTAAGCTGTTTTAGTTTATTTCTATAAGCTCTTTAGAACAGCTTATTAAAAAGCTTATAGCTTATATGAAAATAGTTTGACTATACTTTTTCTTTTATTCTATAAATAGTTCATACATAAGCACTTATATGATAAGTTAAACTGTTTATCCAAATAGGGCCTGCTAATTGTCAATATCCTCTTATGTTTGTTACAGTATGGTGTCTCACTTGAGTAAGTTCAGGTTATCAGTCATCACACTATCCCTTTACATGTTCTTTATTAGATCTATCTTGGTCTTTTGAATATCTAGTTTTTGGATCTTTTGGTCAGTATTCAGATTGGGCTTTCCTGCCTCATTGACTCCAGTTTGTGGTGGTGGGGTCATGGCTTGATTACTTGAATACAACCTTTGGATAAGAGCCTCTAATACTCTATAGTTTATGCCCATAAAGATCATGTGCATTGCATGCAACATGTTGTATTATCGGCATGCAGTTTATCATGATTGTTTCTTATAAGGATTTCAAATTGCTATAAGAGCAAAGTTTCAAGCCATATGATATATACAACAAACTAGAAGCATGTGTGGTATATAATGAAGCGTGCATCTATGCGAAATAAAGACACATTGCTCTGTCGAAACACAAGCACACCTAATTAGCTTAACATTAGAGTTATAGACTTTTCTTTCATTCTGTATTTTCTTTTTTATTTCCACTCCTTCTGTATTGGCACAAGGCCGACAGCAACTCGTATGACGATAGAAAAATAACTCGTAGTAAATCTAACTAAAGCAGTGTTGTAGAAAAAAGCTTCCATAAAGACAGAAAACTTAAGTAGAGAGAAAATTAATTTAAAATGAAGGTGCTTTACTGTTTTTTATAATTCGTTGATGCTTACGTAGAGAGAAAATTAATTTAAAATGAAGATGCTTACTGTTTTTAGTGATCTTTTTTGCAAAAAAAGATAGAGACTTATGGCTTATCCAGATATCCTGTTGTTGCATGTAATAGGGAATCTTGCTCAAGATGTTGTTGCCGTGACTGATTCTACAGCTGCAGCAGCTTCTGTGTCTCTGTTAAATAGGAATGTCGATAATGTTGCTGTTATAGGCTCAGAAATTCAGACAACGGATAGAAACTTACAGATTAGACTGTTAGACCCTTCATCAAACGGTAGAAGAAGAAATGGCGTTTCAAGACAAGTTTCAAATGAACCAACAAGAATATCGAAATGA
Protein sequence

>MS.gene82259.t1

MELDLNQEPLDHQTSTSVRSFDSMLEELESAEENIRNRITHLEAITSRARQRRGLPPNSSRIQITNVTGETATAGDVQGEERREERNDADQDEGIVESVKGGKRKSSHLIAKALGMEEIDNGKVEESSGNFFDCNICLDIARDPVLTCCGHLFCWPCFYQLSYAYSKAKECPVCKGEVTESGIIPIYGHGNGGGDCLMEMKEAGLRVPPRPKAPRVESIRQKLLTQGASSSSIVQSIRRFHNRIGGFGEQVQLESPNSTTPERNNGLPVQSRTQTDNNEHGGSQQVSRLLEQGASSFSSLSTALNSAMDSAERLVQDLESYINGHNTGGSREQNPPTVTRNSTLSVAATNQSESRGQDVVDLTNSVAAAASLSPLSRNVDNVAVAGSQIHTTDRNVQVEEEIALQDKFQMNQEEEEDCDDGLLWNLAQDVVAVTDSTAAAASVSLLNRNVDNVAVIGSEIQTTDRNLQIRLLDPSSNGRRRNGVSRQVSNEPTRISK