AlfalfaGEDB | MS.gene84390

M. sativa cultivar XinJiangDaYe / MS.gene84390

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene84390.t1	KEH23142.1	94.9	257	13	0	1	257	1	257	3.20E-108	401.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene84390.t1	A0A072U0F6	94.9	257	13	0	1	257	1	257	2.3e-108	401.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049150	MS.gene84390	0.805096	1.57E-49	-1.69E-46
MS.gene049153	MS.gene84390	0.806758	7.04E-50	-1.69E-46
MS.gene049297	MS.gene84390	0.830053	3.65E-55	-1.69E-46
MS.gene049346	MS.gene84390	0.849564	2.98E-60	-1.69E-46
MS.gene049378	MS.gene84390	0.811869	5.64E-51	-1.69E-46
MS.gene049599	MS.gene84390	-0.811406	7.11E-51	-1.69E-46
MS.gene049758	MS.gene84390	0.849322	3.48E-60	-1.69E-46
MS.gene049910	MS.gene84390	0.854567	1.13E-61	-1.69E-46
MS.gene049915	MS.gene84390	0.825787	3.87E-54	-1.69E-46
MS.gene050226	MS.gene84390	0.80657	7.72E-50	-1.69E-46
MS.gene050227	MS.gene84390	0.824545	7.62E-54	-1.69E-46
MS.gene050228	MS.gene84390	0.818324	2.08E-52	-1.69E-46
MS.gene050301	MS.gene84390	0.847122	1.41E-59	-1.69E-46
MS.gene050330	MS.gene84390	-0.817731	2.83E-52	-1.69E-46
MS.gene050397	MS.gene84390	0.837707	4.43E-57	-1.69E-46
MS.gene050404	MS.gene84390	0.808097	3.66E-50	-1.69E-46
MS.gene050406	MS.gene84390	0.831517	1.60E-55	-1.69E-46
MS.gene050426	MS.gene84390	-0.815052	1.13E-51	-1.69E-46
MS.gene050464	MS.gene84390	0.842658	2.26E-58	-1.69E-46
MS.gene050529	MS.gene84390	0.842049	3.28E-58	-1.69E-46
MS.gene050558	MS.gene84390	0.826513	2.60E-54	-1.69E-46
MS.gene050642	MS.gene84390	0.859875	3.05E-63	-1.69E-46
MS.gene050670	MS.gene84390	0.810667	1.03E-50	-1.69E-46
MS.gene050820	MS.gene84390	0.840961	6.34E-58	-1.69E-46
MS.gene05119	MS.gene84390	-0.818487	1.91E-52	-1.69E-46
MS.gene051471	MS.gene84390	0.806647	7.43E-50	-1.69E-46
MS.gene051582	MS.gene84390	0.832733	7.99E-56	-1.69E-46
MS.gene051860	MS.gene84390	0.849528	3.05E-60	-1.69E-46
MS.gene051983	MS.gene84390	0.853393	2.46E-61	-1.69E-46
MS.gene052011	MS.gene84390	-0.848656	5.33E-60	-1.69E-46
MS.gene052077	MS.gene84390	0.857838	1.24E-62	-1.69E-46
MS.gene052078	MS.gene84390	0.815976	7.01E-52	-1.69E-46
MS.gene052176	MS.gene84390	0.817918	2.57E-52	-1.69E-46
MS.gene052188	MS.gene84390	0.813953	1.97E-51	-1.69E-46
MS.gene052253	MS.gene84390	0.85014	2.06E-60	-1.69E-46
MS.gene052309	MS.gene84390	-0.817411	3.34E-52	-1.69E-46
MS.gene052310	MS.gene84390	0.801784	7.65E-49	-1.69E-46
MS.gene052340	MS.gene84390	0.831756	1.39E-55	-1.69E-46
MS.gene053557	MS.gene84390	0.802039	6.78E-49	-1.69E-46
MS.gene053836	MS.gene84390	0.899446	1.97E-77	-1.69E-46
MS.gene053907	MS.gene84390	0.82025	7.58E-53	-1.69E-46
MS.gene053971	MS.gene84390	0.840011	1.12E-57	-1.69E-46
MS.gene054183	MS.gene84390	0.834129	3.58E-56	-1.69E-46
MS.gene054349	MS.gene84390	0.800214	1.60E-48	-1.69E-46
MS.gene054473	MS.gene84390	0.875528	2.87E-68	-1.69E-46
MS.gene054542	MS.gene84390	0.800908	1.16E-48	-1.69E-46
MS.gene054667	MS.gene84390	0.810739	9.92E-51	-1.69E-46
MS.gene054918	MS.gene84390	0.814663	1.37E-51	-1.69E-46
MS.gene054930	MS.gene84390	0.850535	1.60E-60	-1.69E-46
MS.gene054942	MS.gene84390	0.817247	3.64E-52	-1.69E-46
MS.gene054983	MS.gene84390	0.975403	6.87E-140	-1.69E-46
MS.gene055116	MS.gene84390	0.820763	5.78E-53	-1.69E-46
MS.gene055277	MS.gene84390	0.805879	1.08E-49	-1.69E-46
MS.gene055422	MS.gene84390	0.835179	1.95E-56	-1.69E-46
MS.gene055428	MS.gene84390	0.869332	3.35E-66	-1.69E-46
MS.gene05553	MS.gene84390	0.83638	9.68E-57	-1.69E-46
MS.gene055712	MS.gene84390	0.813314	2.73E-51	-1.69E-46
MS.gene055859	MS.gene84390	0.804074	2.57E-49	-1.69E-46
MS.gene056407	MS.gene84390	0.808278	3.35E-50	-1.69E-46
MS.gene056451	MS.gene84390	0.822292	2.56E-53	-1.69E-46
MS.gene056612	MS.gene84390	0.857833	1.25E-62	-1.69E-46
MS.gene056723	MS.gene84390	0.82238	2.44E-53	-1.69E-46
MS.gene056899	MS.gene84390	-0.838412	2.92E-57	-1.69E-46
MS.gene056979	MS.gene84390	0.839712	1.35E-57	-1.69E-46
MS.gene05702	MS.gene84390	0.858287	9.14E-63	-1.69E-46
MS.gene057121	MS.gene84390	0.86165	8.84E-64	-1.69E-46
MS.gene057245	MS.gene84390	0.845809	3.22E-59	-1.69E-46
MS.gene05733	MS.gene84390	0.809491	1.84E-50	-1.69E-46
MS.gene057439	MS.gene84390	0.805336	1.40E-49	-1.69E-46
MS.gene057639	MS.gene84390	0.827003	1.99E-54	-1.69E-46
MS.gene058002	MS.gene84390	0.8646	1.08E-64	-1.69E-46
MS.gene05811	MS.gene84390	0.844583	6.91E-59	-1.69E-46
MS.gene058142	MS.gene84390	0.858327	8.89E-63	-1.69E-46
MS.gene058233	MS.gene84390	0.841373	4.94E-58	-1.69E-46
MS.gene058276	MS.gene84390	0.821045	4.97E-53	-1.69E-46
MS.gene058660	MS.gene84390	0.814032	1.89E-51	-1.69E-46
MS.gene058710	MS.gene84390	0.848448	6.09E-60	-1.69E-46
MS.gene058749	MS.gene84390	0.839892	1.21E-57	-1.69E-46
MS.gene058845	MS.gene84390	0.806703	7.23E-50	-1.69E-46
MS.gene05907	MS.gene84390	0.845631	3.60E-59	-1.69E-46
MS.gene059247	MS.gene84390	0.806764	7.02E-50	-1.69E-46
MS.gene059285	MS.gene84390	0.839451	1.57E-57	-1.69E-46
MS.gene059420	MS.gene84390	0.820397	7.01E-53	-1.69E-46
MS.gene059518	MS.gene84390	0.817612	3.01E-52	-1.69E-46
MS.gene059597	MS.gene84390	0.829492	4.99E-55	-1.69E-46
MS.gene059688	MS.gene84390	0.80364	3.16E-49	-1.69E-46
MS.gene059692	MS.gene84390	0.816498	5.36E-52	-1.69E-46
MS.gene059695	MS.gene84390	0.811704	6.13E-51	-1.69E-46
MS.gene05984	MS.gene84390	0.879904	8.51E-70	-1.69E-46
MS.gene059894	MS.gene84390	0.856923	2.32E-62	-1.69E-46
MS.gene059899	MS.gene84390	0.815664	8.23E-52	-1.69E-46
MS.gene06037	MS.gene84390	0.8035	3.38E-49	-1.69E-46
MS.gene060403	MS.gene84390	0.802309	5.96E-49	-1.69E-46
MS.gene060407	MS.gene84390	0.80274	4.86E-49	-1.69E-46
MS.gene061040	MS.gene84390	-0.80184	7.45E-49	-1.69E-46
MS.gene061049	MS.gene84390	0.885203	9.95E-72	-1.69E-46
MS.gene061114	MS.gene84390	-0.823757	1.17E-53	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene84390.t1	MTR_7g067000	96.109	257	10	0	1	257	1	257	1.06e-178	491
MS.gene84390.t1	MTR_4g015010	59.127	252	90	6	1	239	14	265	7.96e-89	265

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene84390.t1	AT3G09050	60.938	192	73	2	49	240	56	245	1.53e-77	235

Find 36 sgRNAs with CRISPR-Local

Find 53 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCATTGTTGGTCTTTCTTA+TGG	0.167344	7.3:-37857657	None:intergenic
TCTGCATACTTAACCCAATT+TGG	0.242948	7.3:+37857071	MS.gene84390:CDS
AATGTTTACCTCTTCTTCAT+TGG	0.306206	7.3:+37857497	MS.gene84390:CDS
TAAAGGCTGGTAGCGGAATC+AGG	0.365890	7.3:+37857471	MS.gene84390:CDS
CTTGATGTTGTTCAGTTTGA+AGG	0.387068	7.3:-37856868	None:intergenic
TTGCGTCGCCTTCGACTGAT+TGG	0.388513	7.3:+37857549	MS.gene84390:CDS
AGCGGAGTTTGAAGTTCAAA+TGG	0.433795	7.3:+37857337	MS.gene84390:CDS
AACGCCGAGTTTGAAAAGAT+TGG	0.435378	7.3:+37857104	MS.gene84390:CDS
TGTGCCTCTGCAAGTCCAAT+GGG	0.448799	7.3:+37856937	MS.gene84390:CDS
CAGTTTGAAGGAAGGATGTA+GGG	0.450731	7.3:-37856856	None:intergenic
TCCGCTACCAGCCTTTAGTC+TGG	0.451103	7.3:-37857465	None:intergenic
TCAGTTTGAAGGAAGGATGT+AGG	0.456859	7.3:-37856857	None:intergenic
TTGTGCCTCTGCAAGTCCAA+TGG	0.464033	7.3:+37856936	MS.gene84390:CDS
ATTCCTCGAATTCTAGCATC+TGG	0.480236	7.3:-37857268	None:intergenic
TTTGAAGTTCAAATGGAAAA+CGG	0.485912	7.3:+37857344	MS.gene84390:CDS
CTTTAAGAACCTCAGAAAGA+AGG	0.494681	7.3:+37857385	MS.gene84390:CDS
TCTCATCTAGATATTCTGTA+AGG	0.518089	7.3:-37857046	None:intergenic
AAACTACTGTAAAGGTCAGC+AGG	0.526715	7.3:-37857520	None:intergenic
ATCTGCAGAAATTAACAGAC+AGG	0.528683	7.3:+37857289	MS.gene84390:CDS
AGCTTGAGAATCAGACCCAT+TGG	0.534125	7.3:-37856952	None:intergenic
GAGCCAGATGCTAGAATTCG+AGG	0.536260	7.3:+37857265	MS.gene84390:CDS
CAACAGGTACCTTCTTTCTG+AGG	0.538228	7.3:-37857394	None:intergenic
AATAGAATAAACTACTGTAA+AGG	0.541708	7.3:-37857528	None:intergenic
TAAAGATGTCACCAGACTAA+AGG	0.542082	7.3:+37857454	MS.gene84390:CDS
GATTCTCAAGCTCCTCAACC+AGG	0.562806	7.3:+37856963	MS.gene84390:CDS
AGAGCGTGAAAACGAATTAG+CGG	0.567801	7.3:+37857319	MS.gene84390:CDS
ATGGAAAACGGCAGAAACGA+AGG	0.568917	7.3:+37857356	MS.gene84390:CDS
ACCAGACTAAAGGCTGGTAG+CGG	0.574115	7.3:+37857464	MS.gene84390:CDS
TCAGCAGGCCAATGAAGAAG+AGG	0.579281	7.3:-37857505	None:intergenic
TAAAGCAAAAGCCAAAGAAG+AGG	0.598747	7.3:+37857421	MS.gene84390:CDS
GATGTCACCAGACTAAAGGC+TGG	0.604550	7.3:+37857458	MS.gene84390:CDS
TTAACAGATAACAGCAAACA+TGG	0.611396	7.3:+37857241	MS.gene84390:intron
ATGTTGTTCAGTTTGAAGGA+AGG	0.625091	7.3:-37856864	None:intergenic
TGGAAAACGGCAGAAACGAA+GGG	0.629953	7.3:+37857357	MS.gene84390:CDS
CAGACCCATTGGACTTGCAG+AGG	0.674115	7.3:-37856941	None:intergenic
CATCTAGATATTCTGTAAGG+CGG	0.677478	7.3:-37857043	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTTTATTTACATTTTACA+TGG	-	chr7.3:37857197-37857216	None:intergenic	10.0%
!!	AAATACAAAGTGAATATAAG+AGG	-	chr7.3:37857223-37857242	None:intergenic	20.0%
!!	AATAGAATAAACTACTGTAA+AGG	-	chr7.3:37857531-37857550	None:intergenic	20.0%
!!	TTTGAAGTTCAAATGGAAAA+CGG	+	chr7.3:37857344-37857363	MS.gene84390:CDS	25.0%
!!!	TTTGTTTTCTTCTTCATTGT+TGG	-	chr7.3:37857672-37857691	None:intergenic	25.0%
	AATGTTTACCTCTTCTTCAT+TGG	+	chr7.3:37857497-37857516	MS.gene84390:CDS	30.0%
	TCTCATCTAGATATTCTGTA+AGG	-	chr7.3:37857049-37857068	None:intergenic	30.0%
	TTAACAGATAACAGCAAACA+TGG	+	chr7.3:37857241-37857260	MS.gene84390:intron	30.0%
!	TTCTCCAATCTTTTCAAACT+CGG	-	chr7.3:37857111-37857130	None:intergenic	30.0%
!!	TTCATTGTTGGTCTTTCTTA+TGG	-	chr7.3:37857660-37857679	None:intergenic	30.0%
!!!	AATTTTTTCTGCCTCTTCTT+TGG	-	chr7.3:37857435-37857454	None:intergenic	30.0%
!!!	GTTGACTTTTTCACCAAATT+GGG	-	chr7.3:37857087-37857106	None:intergenic	30.0%
!!!	TCTTGTTTTTTTGTATCTCC+TGG	-	chr7.3:37856984-37857003	None:intergenic	30.0%
	ATCTGCAGAAATTAACAGAC+AGG	+	chr7.3:37857289-37857308	MS.gene84390:CDS	35.0%
	ATGTTGTTCAGTTTGAAGGA+AGG	-	chr7.3:37856867-37856886	None:intergenic	35.0%
	CATCTAGATATTCTGTAAGG+CGG	-	chr7.3:37857046-37857065	None:intergenic	35.0%
	CTTGATGTTGTTCAGTTTGA+AGG	-	chr7.3:37856871-37856890	None:intergenic	35.0%
	TAAAGATGTCACCAGACTAA+AGG	+	chr7.3:37857454-37857473	MS.gene84390:CDS	35.0%
	TAAAGCAAAAGCCAAAGAAG+AGG	+	chr7.3:37857421-37857440	MS.gene84390:CDS	35.0%
	TCTGCATACTTAACCCAATT+TGG	+	chr7.3:37857071-37857090	MS.gene84390:CDS	35.0%
!	CTTTAAGAACCTCAGAAAGA+AGG	+	chr7.3:37857385-37857404	MS.gene84390:CDS	35.0%
!	GCTTTTGCTTTATCAACAAC+AGG	-	chr7.3:37857413-37857432	None:intergenic	35.0%
!	TGGAGAAAAGTTGCAGTTTT+TGG	+	chr7.3:37857569-37857588	MS.gene84390:CDS	35.0%
!	TGTTGTAAAGTCTTTTGCAG+TGG	-	chr7.3:37856902-37856921	None:intergenic	35.0%
!!!	AGTTTTTGGAGCAATTCTTG+TGG	+	chr7.3:37857583-37857602	MS.gene84390:CDS	35.0%
!!!	CGTTGACTTTTTCACCAAAT+TGG	-	chr7.3:37857088-37857107	None:intergenic	35.0%
!!!	TTTTTTGTATCTCCTGGTTG+AGG	-	chr7.3:37856978-37856997	None:intergenic	35.0%
	AAACTACTGTAAAGGTCAGC+AGG	-	chr7.3:37857523-37857542	None:intergenic	40.0%
	AACGCCGAGTTTGAAAAGAT+TGG	+	chr7.3:37857104-37857123	MS.gene84390:CDS	40.0%
	AGAGCGTGAAAACGAATTAG+CGG	+	chr7.3:37857319-37857338	MS.gene84390:CDS	40.0%
	ATTCCTCGAATTCTAGCATC+TGG	-	chr7.3:37857271-37857290	None:intergenic	40.0%
	CAGTTTGAAGGAAGGATGTA+GGG	-	chr7.3:37856859-37856878	None:intergenic	40.0%
	TCAGTTTGAAGGAAGGATGT+AGG	-	chr7.3:37856860-37856879	None:intergenic	40.0%
!	ACTTTTCTCCAATCAGTCGA+AGG	-	chr7.3:37857560-37857579	None:intergenic	40.0%
!	AGCGGAGTTTGAAGTTCAAA+TGG	+	chr7.3:37857337-37857356	MS.gene84390:CDS	40.0%
!	TTACATGGTTAGCTCGAAAG+TGG	-	chr7.3:37857182-37857201	None:intergenic	40.0%
!!!	GACTTTTCGAGTTTGTAGCA+TGG	-	chr7.3:37857024-37857043	None:intergenic	40.0%
	AAGTGGAAGCAAGCATACTC+TGG	-	chr7.3:37857165-37857184	None:intergenic	45.0%
	AGCTTGAGAATCAGACCCAT+TGG	-	chr7.3:37856955-37856974	None:intergenic	45.0%
	ATGGAAAACGGCAGAAACGA+AGG	+	chr7.3:37857356-37857375	MS.gene84390:CDS	45.0%
	CAACAGGTACCTTCTTTCTG+AGG	-	chr7.3:37857397-37857416	None:intergenic	45.0%
	TGGAAAACGGCAGAAACGAA+GGG	+	chr7.3:37857357-37857376	MS.gene84390:CDS	45.0%
	ACCAGACTAAAGGCTGGTAG+CGG	+	chr7.3:37857464-37857483	MS.gene84390:CDS	50.0%
	GAGCCAGATGCTAGAATTCG+AGG	+	chr7.3:37857265-37857284	MS.gene84390:CDS	50.0%
	GATGTCACCAGACTAAAGGC+TGG	+	chr7.3:37857458-37857477	MS.gene84390:CDS	50.0%
	GATTCTCAAGCTCCTCAACC+AGG	+	chr7.3:37856963-37856982	MS.gene84390:CDS	50.0%
	TCAGCAGGCCAATGAAGAAG+AGG	-	chr7.3:37857508-37857527	None:intergenic	50.0%
	TGTGCCTCTGCAAGTCCAAT+GGG	+	chr7.3:37856937-37856956	MS.gene84390:CDS	50.0%
	TTGTGCCTCTGCAAGTCCAA+TGG	+	chr7.3:37856936-37856955	MS.gene84390:CDS	50.0%
!!	TAAAGGCTGGTAGCGGAATC+AGG	+	chr7.3:37857471-37857490	MS.gene84390:CDS	50.0%
	CAGACCCATTGGACTTGCAG+AGG	-	chr7.3:37856944-37856963	None:intergenic	55.0%
	TCCGCTACCAGCCTTTAGTC+TGG	-	chr7.3:37857468-37857487	None:intergenic	55.0%
	TTGCGTCGCCTTCGACTGAT+TGG	+	chr7.3:37857549-37857568	MS.gene84390:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.3	gene	37856840	37857694	37856840	ID=MS.gene84390
chr7.3	mRNA	37856840	37857694	37856840	ID=MS.gene84390.t1;Parent=MS.gene84390
chr7.3	exon	37856840	37857166	37856840	ID=MS.gene84390.t1.exon1;Parent=MS.gene84390.t1
chr7.3	CDS	37856840	37857166	37856840	ID=cds.MS.gene84390.t1;Parent=MS.gene84390.t1
chr7.3	exon	37857248	37857694	37857248	ID=MS.gene84390.t1.exon2;Parent=MS.gene84390.t1
chr7.3	CDS	37857248	37857694	37857248	ID=cds.MS.gene84390.t1;Parent=MS.gene84390.t1

Gene Sequence

>MS.gene84390

ATGATTGCTCTCAAATCCCTACATCCTTCCTTCAAACTGAACAACATCAAGTGTAATATCCACTGCAAAAGACTTTACAACACAAGAAGTTATACTTTGTGCCTCTGCAAGTCCAATGGGTCTGATTCTCAAGCTCCTCAACCAGGAGATACAAAAAAACAAGAACTGCTAGCACAGATTGCCATGCTACAAACTCGAAAAGTCCGCCTTACAGAATATCTAGATGAGAGATCTGCATACTTAACCCAATTTGGTGAAAAAGTCAACGCCGAGTTTGAAAAGATTGGAGAAGATGCACTCAAAGAATTAGATGAAGCTAGTGCCAGAGTATGCTTGCTTCCACTTTCGAGCTAACCATGTAAAATGTAAATAAAAAACGACCTCTTATATTCACTTTGTATTTAACAGATAACAGCAAACATGGAGAGCCAGATGCTAGAATTCGAGGAATCTGCAGAAATTAACAGACAGGAGATTCAAGAGCGTGAAAACGAATTAGCGGAGTTTGAAGTTCAAATGGAAAACGGCAGAAACGAAGGGCTATTCTTTAAGAACCTCAGAAAGAAGGTACCTGTTGTTGATAAAGCAAAAGCCAAAGAAGAGGCAGAAAAAATTAAAGATGTCACCAGACTAAAGGCTGGTAGCGGAATCAGGAAAAATGTTTACCTCTTCTTCATTGGCCTGCTGACCTTTACAGTAGTTTATTCTATTGCGTCGCCTTCGACTGATTGGAGAAAAGTTGCAGTTTTTGGAGCAATTCTTGTGGCTTTGACTTCTCAGTTCGTCTATGAACAAAACATGTCAGCAGACACTAGAACCATAAGAAAGACCAACAATGAAGAAGAAAACAAATGA

Protein sequence

>MS.gene84390.t1

MIALKSLHPSFKLNNIKCNIHCKRLYNTRSYTLCLCKSNGSDSQAPQPGDTKKQELLAQIAMLQTRKVRLTEYLDERSAYLTQFGEKVNAEFEKIGEDALKELDEASARITANMESQMLEFEESAEINRQEIQERENELAEFEVQMENGRNEGLFFKNLRKKVPVVDKAKAKEEAEKIKDVTRLKAGSGIRKNVYLFFIGLLTFTVVYSIASPSTDWRKVAVFGAILVALTSQFVYEQNMSADTRTIRKTNNEEENK