AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680031087.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680031087.01.T01 MTR_3g099777 76.712 219 7 1 1 175 3464 3682 6.26e-104 328
MsG0680031087.01.T01 MTR_5g066710 73.973 219 13 1 1 175 3436 3654 5.26e-101 320
MsG0680031087.01.T01 MTR_4g094708 63.470 219 36 1 1 175 3539 3757 6.94e-86 277
MsG0680031087.01.T01 MTR_4g094705 63.014 219 37 1 1 175 3518 3736 8.42e-86 276
MsG0680031087.01.T01 MTR_2g033040 35.909 220 86 7 2 175 813 1023 2.62e-25 103
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680031087.01.T01 AT1G70320 63.470 219 36 2 1 175 3439 3657 4.10e-85 275
MsG0680031087.01.T01 AT1G55860 63.470 219 36 2 1 175 3462 3680 7.85e-85 274
MsG0680031087.01.T01 AT3G17205 36.364 220 85 8 2 175 804 1014 6.77e-25 102
MsG0680031087.01.T01 AT3G17205 36.364 220 85 8 2 175 818 1028 6.91e-25 102
MsG0680031087.01.T01 AT3G17205 36.364 220 85 8 2 175 818 1028 6.91e-25 102

Find 37 sgRNAs with CRISPR-Local

Find 93 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCTCAAATAAATGCTTTCT+TGG 0.242468 6:+14075685 MsG0680031087.01.T01:CDS
AGCGCACTTCAAGGAATTTC+AGG 0.248103 6:+14076543 MsG0680031087.01.T01:CDS
TCTTCCAAATGTTGTTTAGA+TGG 0.271330 6:-14076793 None:intergenic
CATGAAGCAAATGAGGGATT+TGG 0.287413 6:+14076835 MsG0680031087.01.T01:CDS
TAAGCATCAATATGTTGATT+TGG 0.313697 6:+14075631 MsG0680031087.01.T01:CDS
AACAAACTTACAATCAATAT+CGG 0.331367 6:-14075789 None:intergenic
TTTCAATCAATTAGATTTGC+CGG 0.333489 6:+14076765 MsG0680031087.01.T01:CDS
GAATGATATCAGTGATGTTC+TGG 0.379619 6:+14075372 MsG0680031087.01.T01:CDS
TTTGAAAGAATTATATATAT+GGG 0.392849 6:+14076953 MsG0680031087.01.T01:exon
CAAATAAATGCTTTCTTGGA+AGG 0.404199 6:+14075689 MsG0680031087.01.T01:CDS
ACTGATTATGAGTTGATTCC+CGG 0.416823 6:+14075578 MsG0680031087.01.T01:CDS
TCCAAGAAAGCATTTATTTG+AGG 0.420992 6:-14075686 None:intergenic
GTATCCATCTAAACAACATT+TGG 0.432935 6:+14076789 MsG0680031087.01.T01:CDS
AATGCTTTCTTGGAAGGGTT+CGG 0.435126 6:+14075695 MsG0680031087.01.T01:CDS
TATATTCAATGACAAAGAAC+TGG 0.439692 6:+14075745 MsG0680031087.01.T01:CDS
AATATAGATATCAACTCCTT+CGG 0.481461 6:-14075728 None:intergenic
AACTTTAGTATTCCGTCCAC+CGG 0.490687 6:-14075597 None:intergenic
AAATAAATGCTTTCTTGGAA+GGG 0.491087 6:+14075690 MsG0680031087.01.T01:CDS
AGCAAATGAGGGATTTGGAT+TGG 0.497119 6:+14076840 MsG0680031087.01.T01:CDS
TGCGATTCATGAAGCAAATG+AGG 0.510842 6:+14076828 MsG0680031087.01.T01:CDS
ATGTTTCAGGTGCCTCTAGA+GGG 0.525479 6:+14076516 MsG0680031087.01.T01:intron
AATGTTTCAGGTGCCTCTAG+AGG 0.525907 6:+14076515 MsG0680031087.01.T01:intron
ACAAACTTACAATCAATATC+GGG 0.528402 6:-14075788 None:intergenic
CTAAACAACATTTGGAAGAG+AGG 0.548837 6:+14076797 MsG0680031087.01.T01:CDS
ACTTGCCTTCTGCTCATACA+TGG 0.550247 6:+14076610 MsG0680031087.01.T01:CDS
ATGAGTTGATTCCCGGTGGA+CGG 0.550628 6:+14075585 MsG0680031087.01.T01:CDS
TGGTACCATGTATGAGCAGA+AGG 0.570039 6:-14076615 None:intergenic
TGTTGTTTAGATGGATACTC+CGG 0.574334 6:-14076784 None:intergenic
ACTTTAGTATTCCGTCCACC+GGG 0.596825 6:-14075596 None:intergenic
GCGATTCATGAAGCAAATGA+GGG 0.601962 6:+14076829 MsG0680031087.01.T01:CDS
TTTCAAATACACAAGGCATG+TGG 0.621431 6:+14076576 MsG0680031087.01.T01:CDS
GAACTGGAACTATTGATCAG+TGG 0.637735 6:+14075761 MsG0680031087.01.T01:CDS
GTTCGGTGAAATAATTCCGA+AGG 0.644631 6:+14075712 MsG0680031087.01.T01:CDS
GATTATGAGTTGATTCCCGG+TGG 0.660118 6:+14075581 MsG0680031087.01.T01:CDS
AGTGCGCTAAAACCCTCTAG+AGG 0.690121 6:-14076528 None:intergenic
TCAAAAGTTTCAAATACACA+AGG 0.697802 6:+14076569 MsG0680031087.01.T01:CDS
ACGGAATACTAAAGTTACAG+AGG 0.721312 6:+14075604 MsG0680031087.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTGAAAGAATTATATATAT+GGG + Chr6:14076953-14076972 MsG0680031087.01.T01:exon 10.0%
!!! TATGTATTTTATTTATTGTT+TGG + Chr6:14076889-14076908 MsG0680031087.01.T01:CDS 10.0%
!!! TTTTGAAAGAATTATATATA+TGG + Chr6:14076952-14076971 MsG0680031087.01.T01:exon 10.0%
!! TTTAAAAGATTGAATAATGT+TGG + Chr6:14076412-14076431 MsG0680031087.01.T01:intron 15.0%
!!! ATAGAAAATCACTAAATTTT+GGG - Chr6:14076171-14076190 None:intergenic 15.0%
!!! ATATTGGAAATACTTTTATT+TGG + Chr6:14076209-14076228 MsG0680031087.01.T01:intron 15.0%
!!! TAAACATTTTGATCTTTTAT+TGG + Chr6:14076139-14076158 MsG0680031087.01.T01:intron 15.0%
!!! TTTTATTTATTGTTTGGATT+TGG + Chr6:14076895-14076914 MsG0680031087.01.T01:CDS 15.0%
!! AAAGAAACTCAATAAAGATT+AGG - Chr6:14075826-14075845 None:intergenic 20.0%
!! AACAAACTTACAATCAATAT+CGG - Chr6:14075792-14075811 None:intergenic 20.0%
!! TAATCTGAATGAATATGTTT+AGG + Chr6:14075553-14075572 MsG0680031087.01.T01:intron 20.0%
!!! AAATACTTTTATTTGGAGTT+CGG + Chr6:14076216-14076235 MsG0680031087.01.T01:intron 20.0%
!!! AGAAAAGAAATTTTAACACT+CGG + Chr6:14075939-14075958 MsG0680031087.01.T01:intron 20.0%
!!! AGTGTTAAAATTTCTTTTCT+AGG - Chr6:14075939-14075958 None:intergenic 20.0%
!!! ATTCTATCGACATAATTTTT+GGG + Chr6:14076663-14076682 MsG0680031087.01.T01:intron 20.0%
!!! CATAGAAAATCACTAAATTT+TGG - Chr6:14076172-14076191 None:intergenic 20.0%
!!! TAAAAGTATTTCCAATATGA+AGG - Chr6:14076207-14076226 None:intergenic 20.0%
!!! TATTCTATCGACATAATTTT+TGG + Chr6:14076662-14076681 MsG0680031087.01.T01:intron 20.0%
! AAATAAATGCTTTCTTGGAA+GGG + Chr6:14075690-14075709 MsG0680031087.01.T01:CDS 25.0%
! AACATGTAATGTACGATTAA+TGG + Chr6:14076475-14076494 MsG0680031087.01.T01:intron 25.0%
! AATATAGATATCAACTCCTT+CGG - Chr6:14075731-14075750 None:intergenic 25.0%
! ACAAACTTACAATCAATATC+GGG - Chr6:14075791-14075810 None:intergenic 25.0%
! ACATGTAATGTACGATTAAT+GGG + Chr6:14076476-14076495 MsG0680031087.01.T01:intron 25.0%
! TATATTCAATGACAAAGAAC+TGG + Chr6:14075745-14075764 MsG0680031087.01.T01:CDS 25.0%
! TCAAAAGTTTCAAATACACA+AGG + Chr6:14076569-14076588 MsG0680031087.01.T01:CDS 25.0%
! TGAGAAATTATCCTTCATAT+TGG + Chr6:14076193-14076212 MsG0680031087.01.T01:intron 25.0%
! TTTCAATCAATTAGATTTGC+CGG + Chr6:14076765-14076784 MsG0680031087.01.T01:CDS 25.0%
!! TAAGCATCAATATGTTGATT+TGG + Chr6:14075631-14075650 MsG0680031087.01.T01:CDS 25.0%
!!! ATAATTTTTGGGTCAAGTTT+TGG + Chr6:14076674-14076693 MsG0680031087.01.T01:intron 25.0%
!!! GGTTATACATTTTATTTCAG+TGG + Chr6:14075960-14075979 MsG0680031087.01.T01:intron 25.0%
ACCTCAAATAAATGCTTTCT+TGG + Chr6:14075685-14075704 MsG0680031087.01.T01:CDS 30.0%
AGAGCAAATACAGAATATTC+TGG + Chr6:14075991-14076010 MsG0680031087.01.T01:intron 30.0%
AGATTAGACAGTTAACAATG+AGG - Chr6:14075539-14075558 None:intergenic 30.0%
CAAATAAATGCTTTCTTGGA+AGG + Chr6:14075689-14075708 MsG0680031087.01.T01:CDS 30.0%
GAAGAAAGTAAACAGAAAAC+TGG - Chr6:14076288-14076307 None:intergenic 30.0%
GAATAGAATGACATCAACAA+AGG - Chr6:14075872-14075891 None:intergenic 30.0%
GTATCCATCTAAACAACATT+TGG + Chr6:14076789-14076808 MsG0680031087.01.T01:CDS 30.0%
TCTGTTTACTTTCTTCCAAA+TGG + Chr6:14076292-14076311 MsG0680031087.01.T01:intron 30.0%
TTGAGATGATATTGATGCAT+TGG + Chr6:14075510-14075529 MsG0680031087.01.T01:intron 30.0%
! ATTTTTGCTTCCAATGTTTC+AGG + Chr6:14076503-14076522 MsG0680031087.01.T01:intron 30.0%
! TCTTCCAAATGTTGTTTAGA+TGG - Chr6:14076796-14076815 None:intergenic 30.0%
!! TCCAAGAAAGCATTTATTTG+AGG - Chr6:14075689-14075708 None:intergenic 30.0%
ACCAACTGAAAGTAGAGAAA+TGG - Chr6:14076638-14076657 None:intergenic 35.0%
ACCATTTCTCTACTTTCAGT+TGG + Chr6:14076634-14076653 MsG0680031087.01.T01:intron 35.0%
ACGGAATACTAAAGTTACAG+AGG + Chr6:14075604-14075623 MsG0680031087.01.T01:CDS 35.0%
CTAAACAACATTTGGAAGAG+AGG + Chr6:14076797-14076816 MsG0680031087.01.T01:CDS 35.0%
GAATGATATCAGTGATGTTC+TGG + Chr6:14075372-14075391 MsG0680031087.01.T01:CDS 35.0%
GGTTCATTGTATTGAAGACT+TGG + Chr6:14076433-14076452 MsG0680031087.01.T01:intron 35.0%
TGCATTCTATTCCAATACTC+TGG + Chr6:14076376-14076395 MsG0680031087.01.T01:intron 35.0%
TTTCAAATACACAAGGCATG+TGG + Chr6:14076576-14076595 MsG0680031087.01.T01:CDS 35.0%
! TGTTGTTTAGATGGATACTC+CGG - Chr6:14076787-14076806 None:intergenic 35.0%
!! ACTGATTATGAGTTGATTCC+CGG + Chr6:14075578-14075597 MsG0680031087.01.T01:CDS 35.0%
!! GCTCTGAAAGAAATATACCT+TGG - Chr6:14076117-14076136 None:intergenic 35.0%
!!! TTTTAGCATTGATGCAGATG+AGG + Chr6:14075402-14075421 MsG0680031087.01.T01:CDS 35.0%
AACTTTAGTATTCCGTCCAC+CGG - Chr6:14075600-14075619 None:intergenic 40.0%
AATGCTTTCTTGGAAGGGTT+CGG + Chr6:14075695-14075714 MsG0680031087.01.T01:CDS 40.0%
AGCAAATGAGGGATTTGGAT+TGG + Chr6:14076840-14076859 MsG0680031087.01.T01:CDS 40.0%
CATGAAGCAAATGAGGGATT+TGG + Chr6:14076835-14076854 MsG0680031087.01.T01:CDS 40.0%
CTAGCTCATTTGATTGATGC+TGG + Chr6:14075891-14075910 MsG0680031087.01.T01:intron 40.0%
GAACTGGAACTATTGATCAG+TGG + Chr6:14075761-14075780 MsG0680031087.01.T01:CDS 40.0%
GCGATTCATGAAGCAAATGA+GGG + Chr6:14076829-14076848 MsG0680031087.01.T01:CDS 40.0%
TCCCAAAACCATTGAATCAC+CGG - Chr6:14076032-14076051 None:intergenic 40.0%
TGCGATTCATGAAGCAAATG+AGG + Chr6:14076828-14076847 MsG0680031087.01.T01:CDS 40.0%
TGTTCAAGGTTTCAGCAAAG+AGG + Chr6:14076055-14076074 MsG0680031087.01.T01:intron 40.0%
TTGTATTGAAGACTTGGAGC+TGG + Chr6:14076439-14076458 MsG0680031087.01.T01:intron 40.0%
! GTTCGGTGAAATAATTCCGA+AGG + Chr6:14075712-14075731 MsG0680031087.01.T01:CDS 40.0%
! TTCCAAATGGTCGAGAGTTT+TGG + Chr6:14076305-14076324 MsG0680031087.01.T01:intron 40.0%
!! GTTTTGGTGTATGTGTCACA+TGG + Chr6:14076321-14076340 MsG0680031087.01.T01:intron 40.0%
ACGAGAGAAAGCCAGAGTAT+TGG - Chr6:14076390-14076409 None:intergenic 45.0%
ACTTGCCTTCTGCTCATACA+TGG + Chr6:14076610-14076629 MsG0680031087.01.T01:CDS 45.0%
ACTTTAGTATTCCGTCCACC+GGG - Chr6:14075599-14075618 None:intergenic 45.0%
AGCGCACTTCAAGGAATTTC+AGG + Chr6:14076543-14076562 MsG0680031087.01.T01:CDS 45.0%
AGGTTTCAGCAAAGAGGACA+AGG + Chr6:14076061-14076080 MsG0680031087.01.T01:intron 45.0%
CACCAAAACTCTCGACCATT+TGG - Chr6:14076310-14076329 None:intergenic 45.0%
CCCAAAACCATTGAATCACC+GGG - Chr6:14076031-14076050 None:intergenic 45.0%
TGGTACCATGTATGAGCAGA+AGG - Chr6:14076618-14076637 None:intergenic 45.0%
! AATGTTTCAGGTGCCTCTAG+AGG + Chr6:14076515-14076534 MsG0680031087.01.T01:intron 45.0%
! ATGTTTCAGGTGCCTCTAGA+GGG + Chr6:14076516-14076535 MsG0680031087.01.T01:intron 45.0%
! CCCGGTGATTCAATGGTTTT+GGG + Chr6:14076028-14076047 MsG0680031087.01.T01:intron 45.0%
! TCCCGGTGATTCAATGGTTT+TGG + Chr6:14076027-14076046 MsG0680031087.01.T01:intron 45.0%
!! GATTATGAGTTGATTCCCGG+TGG + Chr6:14075581-14075600 MsG0680031087.01.T01:CDS 45.0%
!! TCTAGAGGCACCTGAAACAT+TGG - Chr6:14076516-14076535 None:intergenic 45.0%
!!! GGTGATTCAATGGTTTTGGG+AGG + Chr6:14076031-14076050 MsG0680031087.01.T01:intron 45.0%
!!! TGGTTTTGGGAGGTTGTTCA+AGG + Chr6:14076041-14076060 MsG0680031087.01.T01:intron 45.0%
AGGCTGTTGCAGTTTGTGAC+AGG + Chr6:14076086-14076105 MsG0680031087.01.T01:intron 50.0%
AGTGCGCTAAAACCCTCTAG+AGG - Chr6:14076531-14076550 None:intergenic 50.0%
GTTTGTGACAGGCACTTCCA+AGG + Chr6:14076097-14076116 MsG0680031087.01.T01:intron 50.0%
TCAGCAAAGAGGACAAGGCT+AGG + Chr6:14076066-14076085 MsG0680031087.01.T01:intron 50.0%
TGAAGACTTGGAGCTGGACT+TGG + Chr6:14076445-14076464 MsG0680031087.01.T01:intron 50.0%
!! ATGAGTTGATTCCCGGTGGA+CGG + Chr6:14075585-14075604 MsG0680031087.01.T01:CDS 50.0%
!! CTGGATATAGTGCTGCTTCC+CGG + Chr6:14076010-14076029 MsG0680031087.01.T01:intron 50.0%
!! GAGGGTTTTAGCGCACTTCA+AGG + Chr6:14076534-14076553 MsG0680031087.01.T01:CDS 50.0%
GCTGCTTCCCGGTGATTCAA+TGG + Chr6:14076021-14076040 MsG0680031087.01.T01:intron 55.0%
Chromosome Type Strat End Strand Name
Chr6 gene 14075367 14076988 14075367 ID=MsG0680031087.01;Name=MsG0680031087.01
Chr6 mRNA 14075367 14076988 14075367 ID=MsG0680031087.01.T01;Parent=MsG0680031087.01;Name=MsG0680031087.01.T01;_AED=0.44;_eAED=0.44;_QI=0|0.66|0.5|1|0.66|0.75|4|31|206
Chr6 exon 14075367 14075453 14075367 ID=MsG0680031087.01.T01:exon:9726;Parent=MsG0680031087.01.T01
Chr6 exon 14075575 14075808 14075575 ID=MsG0680031087.01.T01:exon:9727;Parent=MsG0680031087.01.T01
Chr6 exon 14076525 14076631 14076525 ID=MsG0680031087.01.T01:exon:9728;Parent=MsG0680031087.01.T01
Chr6 exon 14076765 14076988 14076765 ID=MsG0680031087.01.T01:exon:9729;Parent=MsG0680031087.01.T01
Chr6 CDS 14075367 14075453 14075367 ID=MsG0680031087.01.T01:cds;Parent=MsG0680031087.01.T01
Chr6 CDS 14075575 14075808 14075575 ID=MsG0680031087.01.T01:cds;Parent=MsG0680031087.01.T01
Chr6 CDS 14076525 14076631 14076525 ID=MsG0680031087.01.T01:cds;Parent=MsG0680031087.01.T01
Chr6 CDS 14076765 14076957 14076765 ID=MsG0680031087.01.T01:cds;Parent=MsG0680031087.01.T01
Chr6 three_prime_UTR 14076958 14076988 14076958 ID=MsG0680031087.01.T01:three_prime_utr;Parent=MsG0680031087.01.T01
Gene Sequence

>MsG0680031087.01.T01

TTGCAGAATGATATCAGTGATGTTCTGGATCTTACTTTTAGCATTGATGCAGATGAGGAAAAGTTGATTTTATATGAACGAACAGAAGTGACTGATTATGAGTTGATTCCCGGTGGACGGAATACTAAAGTTACAGAGGAGAATAAGCATCAATATGTTGATTTGGTTGCTGAGCATCGATTGACTACTGCTATTCGACCTCAAATAAATGCTTTCTTGGAAGGGTTCGGTGAAATAATTCCGAAGGAGTTGATATCTATATTCAATGACAAAGAACTGGAACTATTGATCAGTGGACTTCCCGATATTGATTGTAAGTTTGTGCCTCTAGAGGGTTTTAGCGCACTTCAAGGAATTTCAGGTGCTCAAAAGTTTCAAATACACAAGGCATGTGGAAGTTCTGATCACTTGCCTTCTGCTCATACATGTTTCAATCAATTAGATTTGCCGGAGTATCCATCTAAACAACATTTGGAAGAGAGGTTACTGCTTGCGATTCATGAAGCAAATGAGGGATTTGGATTGGATGAGATTTTCTTTTGTTCAAAATACTATGTATTTTATTTATTGTTTGGATTTGGATTTATTGTTTTGATTTTTGTATTTTATTCATTGTTTTGA

Protein sequence

>MsG0680031087.01.T01

LQNDISDVLDLTFSIDADEEKLILYERTEVTDYELIPGGRNTKVTEENKHQYVDLVAEHRLTTAIRPQINAFLEGFGEIIPKELISIFNDKELELLISGLPDIDCKFVPLEGFSALQGISGAQKFQIHKACGSSDHLPSAHTCFNQLDLPEYPSKQHLEERLLLAIHEANEGFGLDEIFFCSKYYVFYLLFGFGFIVLIFVFYSLF*