AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280007310.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007310.01.T01 MTR_7g115670 62.069 87 11 1 4 90 103 167 5.48e-29 104
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007310.01.T01 AT1G08640 61.628 86 11 2 5 90 113 176 9.75e-25 94.4

Find 15 sgRNAs with CRISPR-Local

Find 64 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAAAAGTCCACAAACTTTA+TGG 0.072335 2:+13360602 MsG0280007310.01.T01:CDS
CCATAAAGTTTGTGGACTTT+TGG 0.215699 2:-13360602 None:intergenic
CTTGGTCCCCAAGGTATAAT+TGG 0.268204 2:-13360473 None:intergenic
TCAGTTGAGTAATCGGAAAA+AGG 0.325391 2:+13359867 MsG0280007310.01.T01:CDS
TGATGGCTCAGTTGAGTAAT+CGG 0.411340 2:+13359860 MsG0280007310.01.T01:CDS
CAATTCAACCATAAAGTTTG+TGG 0.425453 2:-13360610 None:intergenic
CAAACAGCCAATTATACCTT+GGG 0.467405 2:+13360466 MsG0280007310.01.T01:CDS
GGCATATGACAAAGTTATGA+TGG 0.471615 2:+13359843 MsG0280007310.01.T01:CDS
AAATTGAATGCAGGTTTCAA+AGG 0.532979 2:+13360427 MsG0280007310.01.T01:intron
AAATGATGTGCGCATCAACT+TGG 0.546081 2:+13360982 MsG0280007310.01.T01:CDS
AATGTTGATCCAGCTCGAAA+AGG 0.548403 2:+13359822 None:intergenic
CAATTATACCTTGGGGACCA+AGG 0.553731 2:+13360474 MsG0280007310.01.T01:CDS
ACAAACAGCCAATTATACCT+TGG 0.553845 2:+13360465 MsG0280007310.01.T01:CDS
ATAACAAACCTTGGTCCCCA+AGG 0.616918 2:-13360482 None:intergenic
AAACAGCCAATTATACCTTG+GGG 0.721557 2:+13360467 MsG0280007310.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTAAATACAAATTACTCA+TGG - Chr2:13360734-13360753 None:intergenic 15.0%
!! AAGCAAATAAACAAAATGTT+AGG - Chr2:13360385-13360404 None:intergenic 20.0%
!! AGCAAATAAACAAAATGTTA+GGG - Chr2:13360384-13360403 None:intergenic 20.0%
!! ATAATGATTAATCGTATTCT+TGG - Chr2:13360236-13360255 None:intergenic 20.0%
!!! AATTTTGTATACGAAGAATT+GGG + Chr2:13360151-13360170 MsG0280007310.01.T01:intron 20.0%
!!! ATATTTTCTAAATTGAATGC+AGG + Chr2:13360418-13360437 MsG0280007310.01.T01:intron 20.0%
!!! ATTTATTTTCATCTACAGTT+TGG + Chr2:13360756-13360775 MsG0280007310.01.T01:intron 20.0%
! AAAATGTTAGGGTGAAATAT+TGG - Chr2:13360373-13360392 None:intergenic 25.0%
! AAATGTTAGGGTGAAATATT+GGG - Chr2:13360372-13360391 None:intergenic 25.0%
! AATAATGTCAAAAATCCAAG+TGG - Chr2:13360922-13360941 None:intergenic 25.0%
! AGCAGTAATAAAAGAGTAAT+TGG - Chr2:13360670-13360689 None:intergenic 25.0%
! ATGGCATGTTTAATTCATAA+GGG - Chr2:13360715-13360734 None:intergenic 25.0%
! ATGGTTGAATTGTGTTAATT+AGG + Chr2:13360621-13360640 MsG0280007310.01.T01:intron 25.0%
! TCTTCGTATACAAAATTGAT+AGG - Chr2:13360148-13360167 None:intergenic 25.0%
!! CAATTTTGTATACGAAGAAT+TGG + Chr2:13360150-13360169 MsG0280007310.01.T01:intron 25.0%
!!! AGATACTTCTTAGTTTGTTT+TGG + Chr2:13359986-13360005 MsG0280007310.01.T01:intron 25.0%
!!! CATTGTAATGAAAAGTGTTA+AGG - Chr2:13360204-13360223 None:intergenic 25.0%
AAATTGAATGCAGGTTTCAA+AGG + Chr2:13360427-13360446 MsG0280007310.01.T01:intron 30.0%
AACATGATGAAATTTGACAG+TGG - Chr2:13360838-13360857 None:intergenic 30.0%
AATATAGCATAGATGATGTG+GGG - Chr2:13360327-13360346 None:intergenic 30.0%
AATGTTAGGGTGAAATATTG+GGG - Chr2:13360371-13360390 None:intergenic 30.0%
ACAATATAGCATAGATGATG+TGG - Chr2:13360329-13360348 None:intergenic 30.0%
CAATATAGCATAGATGATGT+GGG - Chr2:13360328-13360347 None:intergenic 30.0%
CAATTCAACCATAAAGTTTG+TGG - Chr2:13360613-13360632 None:intergenic 30.0%
CATGGCATGTTTAATTCATA+AGG - Chr2:13360716-13360735 None:intergenic 30.0%
GTGGGGGAATTATTTATTTA+AGG - Chr2:13360310-13360329 None:intergenic 30.0%
TTGATGTGTTATGCATTCAT+TGG + Chr2:13360044-13360063 MsG0280007310.01.T01:intron 30.0%
! AATTATTGCTGCTTCATCAA+AGG - Chr2:13360074-13360093 None:intergenic 30.0%
! ATTTTCATCTACAGTTTGGT+AGG + Chr2:13360760-13360779 MsG0280007310.01.T01:intron 30.0%
! GTTTTGTGTGCAATTTCAAA+TGG - Chr2:13359946-13359965 None:intergenic 30.0%
! TGGATCATTTAAGGTAAAGT+AGG + Chr2:13359900-13359919 MsG0280007310.01.T01:intron 30.0%
! TGTGACTTTTGGATCATTTA+AGG + Chr2:13359891-13359910 MsG0280007310.01.T01:CDS 30.0%
AAACAGCCAATTATACCTTG+GGG + Chr2:13360467-13360486 MsG0280007310.01.T01:CDS 35.0%
ACAAACAGCCAATTATACCT+TGG + Chr2:13360465-13360484 MsG0280007310.01.T01:CDS 35.0%
ATATAGCATAGATGATGTGG+GGG - Chr2:13360326-13360345 None:intergenic 35.0%
ATGTTAGGGTGAAATATTGG+GGG - Chr2:13360370-13360389 None:intergenic 35.0%
CAAACAGCCAATTATACCTT+GGG + Chr2:13360466-13360485 MsG0280007310.01.T01:CDS 35.0%
CAGTTGAGTAATCGGAAAAA+GGG + Chr2:13359868-13359887 MsG0280007310.01.T01:CDS 35.0%
CCAAAAGTCCACAAACTTTA+TGG + Chr2:13360602-13360621 MsG0280007310.01.T01:CDS 35.0%
GATGAAATTTGACAGTGGAA+AGG - Chr2:13360833-13360852 None:intergenic 35.0%
GGCATATGACAAAGTTATGA+TGG + Chr2:13359843-13359862 MsG0280007310.01.T01:CDS 35.0%
TCAGTTGAGTAATCGGAAAA+AGG + Chr2:13359867-13359886 MsG0280007310.01.T01:CDS 35.0%
TGACAAGCAATAACAAACCT+TGG - Chr2:13360494-13360513 None:intergenic 35.0%
TTGTACTTCGATGCAATTTC+AGG + Chr2:13360941-13360960 MsG0280007310.01.T01:intron 35.0%
TTTGTATACGAAGAATTGGG+AGG + Chr2:13360154-13360173 MsG0280007310.01.T01:intron 35.0%
! AAAGTTTGTGGACTTTTGGA+GGG - Chr2:13360601-13360620 None:intergenic 35.0%
! CCATAAAGTTTGTGGACTTT+TGG - Chr2:13360605-13360624 None:intergenic 35.0%
! TAAAGTTTGTGGACTTTTGG+AGG - Chr2:13360602-13360621 None:intergenic 35.0%
!!! GACATGTTAGTTTTTGTTGC+TGG + Chr2:13360522-13360541 MsG0280007310.01.T01:intron 35.0%
AAATGATGTGCGCATCAACT+TGG + Chr2:13360982-13361001 MsG0280007310.01.T01:CDS 40.0%
CCAAGTGGCTGATAATAGAA+TGG - Chr2:13360907-13360926 None:intergenic 40.0%
CCATTCTATTATCAGCCACT+TGG + Chr2:13360904-13360923 MsG0280007310.01.T01:intron 40.0%
GTGAAATATTGGGGGGATAT+AGG - Chr2:13360362-13360381 None:intergenic 40.0%
GTGTGCAATTTCAAATGGAC+AGG - Chr2:13359941-13359960 None:intergenic 40.0%
TGATGGCTCAGTTGAGTAAT+CGG + Chr2:13359860-13359879 MsG0280007310.01.T01:CDS 40.0%
TGTTAGGGTGAAATATTGGG+GGG - Chr2:13360369-13360388 None:intergenic 40.0%
!! AAGTTTGTGGACTTTTGGAG+GGG - Chr2:13360600-13360619 None:intergenic 40.0%
!! TTTTGGAGGGGGAAATAACA+TGG - Chr2:13360588-13360607 None:intergenic 40.0%
ATAACAAACCTTGGTCCCCA+AGG - Chr2:13360485-13360504 None:intergenic 45.0%
CAATTATACCTTGGGGACCA+AGG + Chr2:13360474-13360493 MsG0280007310.01.T01:CDS 45.0%
CTTGGTCCCCAAGGTATAAT+TGG - Chr2:13360476-13360495 None:intergenic 45.0%
!! AGTTTGTGGACTTTTGGAGG+GGG - Chr2:13360599-13360618 None:intergenic 45.0%
!! CGGAAAAAGGGTGTGACTTT+TGG + Chr2:13359880-13359899 MsG0280007310.01.T01:CDS 45.0%
!! TGTCATATGCCTTTTCGAGC+TGG - Chr2:13359834-13359853 None:intergenic 45.0%
Chromosome Type Strat End Strand Name
Chr2 gene 13359823 13361024 13359823 ID=MsG0280007310.01;Name=MsG0280007310.01
Chr2 mRNA 13359823 13361024 13359823 ID=MsG0280007310.01.T01;Parent=MsG0280007310.01;Name=MsG0280007310.01.T01;_AED=0.48;_eAED=0.63;_QI=0|0.33|0.25|1|0.33|0.5|4|0|90
Chr2 exon 13359823 13359912 13359823 ID=MsG0280007310.01.T01:exon:2511;Parent=MsG0280007310.01.T01
Chr2 exon 13360440 13360495 13360440 ID=MsG0280007310.01.T01:exon:2512;Parent=MsG0280007310.01.T01
Chr2 exon 13360567 13360631 13360567 ID=MsG0280007310.01.T01:exon:2513;Parent=MsG0280007310.01.T01
Chr2 exon 13360963 13361024 13360963 ID=MsG0280007310.01.T01:exon:2514;Parent=MsG0280007310.01.T01
Chr2 CDS 13359823 13359912 13359823 ID=MsG0280007310.01.T01:cds;Parent=MsG0280007310.01.T01
Chr2 CDS 13360440 13360495 13360440 ID=MsG0280007310.01.T01:cds;Parent=MsG0280007310.01.T01
Chr2 CDS 13360567 13360631 13360567 ID=MsG0280007310.01.T01:cds;Parent=MsG0280007310.01.T01
Chr2 CDS 13360963 13361024 13360963 ID=MsG0280007310.01.T01:cds;Parent=MsG0280007310.01.T01
Gene Sequence

>MsG0280007310.01.T01

ATGTTGATCCAGCTCGAAAAGGCATATGACAAAGTTATGATGGCTCAGTTGAGTAATCGGAAAAAGGGTGTGACTTTTGGATCATTTAAGGTTTCAAAGGACATAAAGTATGCTGACAAACAGCCAATTATACCTTGGGGACCAAGTGTGCTTGTGAATTGTCTCCATGTTATTTCCCCCTCCAAAAGTCCACAAACTTTATGGTTGAATTGTTTGCTAAATCAAGTGAAAAATGATGTGCGCATCAACTTGGCAATATCTGCTGTTTTGTGA

Protein sequence

>MsG0280007310.01.T01

MLIQLEKAYDKVMMAQLSNRKKGVTFGSFKVSKDIKYADKQPIIPWGPSVLVNCLHVISPSKSPQTLWLNCLLNQVKNDVRINLAISAVL*