Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041426.01.T01 | XP_003626077.1 | 96.751 | 277 | 8 | 1 | 1 | 276 | 1 | 277 | 0 | 556 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041426.01.T01 | Q5NB11 | 70.763 | 236 | 69 | 0 | 41 | 276 | 55 | 290 | 3.05E-130 | 374 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041426.01.T01 | G7KUN3 | 96.751 | 277 | 8 | 1 | 1 | 276 | 1 | 277 | 0.0 | 556 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048347.01 | MsG0780041426.01 | 0.801939 | 7.107362e-49 | 3.437460e-46 |
| MsG0080049066.01 | MsG0780041426.01 | -0.830487 | 2.857123e-55 | 3.014287e-52 |
| MsG0180000467.01 | MsG0780041426.01 | -0.806527 | 7.878712e-50 | 4.285995e-47 |
| MsG0180000762.01 | MsG0780041426.01 | 0.806688 | 7.285724e-50 | 3.979716e-47 |
| MsG0180001111.01 | MsG0780041426.01 | -0.810642 | 1.041406e-50 | 6.311793e-48 |
| MsG0180002935.01 | MsG0780041426.01 | 0.803028 | 4.238899e-49 | 2.107787e-46 |
| MsG0180003375.01 | MsG0780041426.01 | 0.818428 | 1.970393e-52 | 1.474599e-49 |
| MsG0180003757.01 | MsG0780041426.01 | -0.814804 | 1.278385e-51 | 8.662982e-49 |
| MsG0180003779.01 | MsG0780041426.01 | -0.847196 | 1.347150e-59 | 2.375933e-56 |
| MsG0180003904.01 | MsG0780041426.01 | -0.800314 | 1.527666e-48 | 7.091169e-46 |
| MsG0180004491.01 | MsG0780041426.01 | 0.804642 | 1.958788e-49 | 1.015270e-46 |
| MsG0180004859.01 | MsG0780041426.01 | 0.805514 | 1.286663e-49 | 6.820219e-47 |
| MsG0180005291.01 | MsG0780041426.01 | -0.814731 | 1.327051e-51 | 8.975096e-49 |
| MsG0180005817.01 | MsG0780041426.01 | -0.810064 | 1.388035e-50 | 8.285324e-48 |
| MsG0780040621.01 | MsG0780041426.01 | 0.834247 | 3.347677e-56 | 3.950108e-53 |
| MsG0780040685.01 | MsG0780041426.01 | -0.817647 | 2.958213e-52 | 2.166691e-49 |
| MsG0780041106.01 | MsG0780041426.01 | -0.821463 | 3.984835e-53 | 3.244001e-50 |
| MsG0780041172.01 | MsG0780041426.01 | 0.820123 | 8.101614e-53 | 6.355380e-50 |
| MsG0780041316.01 | MsG0780041426.01 | 0.817082 | 3.964716e-52 | 2.859283e-49 |
| MsG0780041426.01 | MsG0780041556.01 | 0.801647 | 8.159536e-49 | 3.917126e-46 |
| MsG0780041426.01 | MsG0780041620.01 | -0.819357 | 1.211975e-52 | 9.304697e-50 |
| MsG0780041426.01 | MsG0780041720.01 | -0.832700 | 8.139209e-56 | 9.168757e-53 |
| MsG0780041426.01 | MsG0780041783.01 | -0.817555 | 3.103199e-52 | 2.267177e-49 |
| MsG0780041426.01 | MsG0780041809.01 | -0.800258 | 1.567961e-48 | 7.267623e-46 |
| MsG0780041426.01 | MsG0880041855.01 | -0.803680 | 3.105980e-49 | 1.570515e-46 |
| MsG0780041426.01 | MsG0880042694.01 | 0.814710 | 1.341227e-51 | 9.065757e-49 |
| MsG0780041426.01 | MsG0880043185.01 | 0.821560 | 3.783755e-53 | 3.088871e-50 |
| MsG0780041426.01 | MsG0880043345.01 | 0.828638 | 8.044657e-55 | 8.042558e-52 |
| MsG0780041426.01 | MsG0880043364.01 | 0.833245 | 5.958264e-56 | 6.822152e-53 |
| MsG0780041426.01 | MsG0880045645.01 | 0.813426 | 2.575003e-51 | 1.681046e-48 |
| MsG0780041426.01 | MsG0880046212.01 | 0.816076 | 6.662458e-52 | 4.674595e-49 |
| MsG0780041426.01 | MsG0880046469.01 | -0.801004 | 1.104497e-48 | 5.217689e-46 |
| MsG0780041426.01 | MsG0880047318.01 | -0.817566 | 3.086016e-52 | 2.255241e-49 |
| MsG0780041426.01 | MsG0880047628.01 | -0.801657 | 8.119409e-49 | 3.898939e-46 |
| MsG0780041426.01 | MsG0880047714.01 | 0.808515 | 2.981645e-50 | 1.708290e-47 |
| MsG0280011299.01 | MsG0780041426.01 | -0.832972 | 6.966114e-56 | 7.911598e-53 |
| MsG0280011301.01 | MsG0780041426.01 | -0.820568 | 6.402926e-53 | 5.084404e-50 |
| MsG0380011974.01 | MsG0780041426.01 | 0.809766 | 1.609013e-50 | 9.530124e-48 |
| MsG0380012281.01 | MsG0780041426.01 | -0.822022 | 2.958281e-53 | 2.446711e-50 |
| MsG0380012913.01 | MsG0780041426.01 | 0.821107 | 4.813965e-53 | 3.880716e-50 |
| MsG0380012918.01 | MsG0780041426.01 | 0.809700 | 1.662183e-50 | 9.827553e-48 |
| MsG0380013272.01 | MsG0780041426.01 | 0.838632 | 2.562316e-57 | 3.452579e-54 |
| MsG0380014410.01 | MsG0780041426.01 | 0.815795 | 7.698148e-52 | 5.360011e-49 |
| MsG0680031464.01 | MsG0780041426.01 | 0.814802 | 1.279876e-51 | 8.672530e-49 |
| MsG0680034229.01 | MsG0780041426.01 | -0.813615 | 2.339728e-51 | 1.535347e-48 |
| MsG0480018626.01 | MsG0780041426.01 | -0.826953 | 2.044152e-54 | 1.945688e-51 |
| MsG0480019923.01 | MsG0780041426.01 | 0.815687 | 8.135863e-52 | 5.647929e-49 |
| MsG0480020814.01 | MsG0780041426.01 | 0.802179 | 6.342122e-49 | 3.086188e-46 |
| MsG0480021205.01 | MsG0780041426.01 | -0.807610 | 4.647079e-50 | 2.599852e-47 |
| MsG0480022584.01 | MsG0780041426.01 | -0.806055 | 9.904076e-50 | 5.323581e-47 |
| MsG0480022765.01 | MsG0780041426.01 | -0.803248 | 3.816774e-49 | 1.908658e-46 |
| MsG0480023179.01 | MsG0780041426.01 | 0.802043 | 6.765610e-49 | 3.280898e-46 |
| MsG0480023187.01 | MsG0780041426.01 | -0.807856 | 4.120998e-50 | 2.320337e-47 |
| MsG0580024140.01 | MsG0780041426.01 | -0.807817 | 4.199422e-50 | 2.362183e-47 |
| MsG0580024220.01 | MsG0780041426.01 | 0.809863 | 1.533092e-50 | 9.103125e-48 |
| MsG0580024309.01 | MsG0780041426.01 | -0.810181 | 1.309476e-50 | 7.839973e-48 |
| MsG0580024571.01 | MsG0780041426.01 | -0.812825 | 3.488708e-51 | 2.240725e-48 |
| MsG0580024678.01 | MsG0780041426.01 | -0.816567 | 5.173641e-52 | 3.678893e-49 |
| MsG0580025000.01 | MsG0780041426.01 | 0.813511 | 2.466482e-51 | 1.613965e-48 |
| MsG0580025023.01 | MsG0780041426.01 | 0.822296 | 2.555047e-53 | 2.130036e-50 |
| MsG0580025117.01 | MsG0780041426.01 | 0.814778 | 1.295380e-51 | 8.771823e-49 |
| MsG0580025486.01 | MsG0780041426.01 | -0.801235 | 9.906371e-49 | 4.706930e-46 |
| MsG0380015111.01 | MsG0780041426.01 | -0.813869 | 2.057225e-51 | 1.359251e-48 |
| MsG0380015619.01 | MsG0780041426.01 | -0.811651 | 6.293680e-51 | 3.917512e-48 |
| MsG0380015752.01 | MsG0780041426.01 | -0.825497 | 4.540157e-54 | 4.145092e-51 |
| MsG0380015971.01 | MsG0780041426.01 | -0.808773 | 2.626879e-50 | 1.515098e-47 |
| MsG0380016229.01 | MsG0780041426.01 | -0.804218 | 2.401081e-49 | 1.230974e-46 |
| MsG0380016424.01 | MsG0780041426.01 | -0.814751 | 1.313446e-51 | 8.887645e-49 |
| MsG0380016717.01 | MsG0780041426.01 | 0.803533 | 3.332042e-49 | 1.678587e-46 |
| MsG0380016790.01 | MsG0780041426.01 | -0.809969 | 1.454767e-50 | 8.662193e-48 |
| MsG0380016839.01 | MsG0780041426.01 | 0.803499 | 3.386711e-49 | 1.704594e-46 |
| MsG0380016931.01 | MsG0780041426.01 | -0.839081 | 1.961194e-57 | 2.678779e-54 |
| MsG0380016934.01 | MsG0780041426.01 | -0.825810 | 3.826784e-54 | 3.525378e-51 |
| MsG0380016935.01 | MsG0780041426.01 | -0.832930 | 7.136838e-56 | 8.094797e-53 |
| MsG0380016960.01 | MsG0780041426.01 | -0.813571 | 2.392652e-51 | 1.568148e-48 |
| MsG0380017579.01 | MsG0780041426.01 | 0.826606 | 2.473303e-54 | 2.330533e-51 |
| MsG0380017690.01 | MsG0780041426.01 | 0.814366 | 1.598457e-51 | 1.070445e-48 |
| MsG0380017895.01 | MsG0780041426.01 | -0.806791 | 6.928015e-50 | 3.794521e-47 |
| MsG0380017962.01 | MsG0780041426.01 | -0.809651 | 1.703057e-50 | 1.005634e-47 |
| MsG0480018440.01 | MsG0780041426.01 | 0.801456 | 8.928964e-49 | 4.266003e-46 |
| MsG0580028510.01 | MsG0780041426.01 | 0.854040 | 1.603749e-61 | 3.535645e-58 |
| MsG0580029172.01 | MsG0780041426.01 | -0.801910 | 7.203618e-49 | 3.481624e-46 |
| MsG0580029177.01 | MsG0780041426.01 | -0.803260 | 3.794988e-49 | 1.898327e-46 |
| MsG0680030635.01 | MsG0780041426.01 | -0.851815 | 6.940306e-61 | 1.421713e-57 |
| MsG0280007216.01 | MsG0780041426.01 | 0.817160 | 3.808590e-52 | 2.752627e-49 |
| MsG0280007233.01 | MsG0780041426.01 | -0.814881 | 1.229231e-51 | 8.346810e-49 |
| MsG0280009167.01 | MsG0780041426.01 | 0.813308 | 2.733156e-51 | 1.778571e-48 |
| MsG0280009742.01 | MsG0780041426.01 | -0.809444 | 1.886677e-50 | 1.107889e-47 |
| MsG0280010410.01 | MsG0780041426.01 | 0.842087 | 3.202331e-58 | 4.802218e-55 |
| MsG0680035841.01 | MsG0780041426.01 | 0.845143 | 4.879132e-59 | 8.053944e-56 |
| MsG0780037751.01 | MsG0780041426.01 | 0.833337 | 5.651375e-56 | 6.489238e-53 |
| MsG0780038922.01 | MsG0780041426.01 | 0.820224 | 7.679471e-53 | 6.040425e-50 |
| MsG0780039821.01 | MsG0780041426.01 | -0.827690 | 1.360872e-54 | 1.323371e-51 |
| MsG0780039836.01 | MsG0780041426.01 | -0.802395 | 5.726187e-49 | 2.801991e-46 |
| MsG0780040034.01 | MsG0780041426.01 | 0.803379 | 3.585846e-49 | 1.799178e-46 |
| MsG0780040072.01 | MsG0780041426.01 | -0.809013 | 2.334106e-50 | 1.354894e-47 |
| MsG0780040121.01 | MsG0780041426.01 | -0.809534 | 1.804335e-50 | 1.062109e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041426.01.T01 | MTR_7g110780 | 96.751 | 277 | 8 | 1 | 1 | 276 | 1 | 277 | 0.0 | 556 |
| MsG0780041426.01.T01 | MTR_3g118390 | 54.545 | 242 | 109 | 1 | 35 | 276 | 126 | 366 | 4.92e-97 | 290 |
| MsG0780041426.01.T01 | MTR_8g074350 | 53.719 | 242 | 110 | 1 | 35 | 276 | 80 | 319 | 2.52e-94 | 281 |
| MsG0780041426.01.T01 | MTR_8g074330 | 50.413 | 242 | 118 | 1 | 35 | 276 | 80 | 319 | 9.66e-89 | 266 |
| MsG0780041426.01.T01 | MTR_8g074335 | 50.413 | 242 | 105 | 1 | 35 | 276 | 82 | 308 | 3.23e-85 | 257 |
| MsG0780041426.01.T01 | MTR_2g099470 | 38.397 | 237 | 110 | 6 | 35 | 270 | 81 | 282 | 4.35e-52 | 172 |
| MsG0780041426.01.T01 | MTR_7g021640 | 38.340 | 253 | 147 | 4 | 33 | 276 | 58 | 310 | 8.00e-52 | 172 |
| MsG0780041426.01.T01 | MTR_2g034680 | 38.492 | 252 | 145 | 6 | 35 | 276 | 56 | 307 | 5.66e-51 | 170 |
| MsG0780041426.01.T01 | MTR_2g099465 | 37.238 | 239 | 115 | 6 | 33 | 270 | 50 | 254 | 9.58e-49 | 162 |
| MsG0780041426.01.T01 | MTR_1g105535 | 37.607 | 234 | 142 | 3 | 33 | 262 | 61 | 294 | 1.10e-48 | 164 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041426.01.T01 | AT4G01700 | 69.173 | 266 | 82 | 0 | 11 | 276 | 14 | 279 | 5.46e-145 | 408 |
| MsG0780041426.01.T01 | AT1G02360 | 66.912 | 272 | 78 | 2 | 9 | 276 | 60 | 323 | 4.60e-139 | 395 |
| MsG0780041426.01.T01 | AT1G02360 | 71.901 | 242 | 68 | 0 | 35 | 276 | 30 | 271 | 4.92e-139 | 392 |
| MsG0780041426.01.T01 | AT1G02360 | 66.912 | 272 | 78 | 2 | 9 | 276 | 54 | 317 | 5.53e-139 | 394 |
| MsG0780041426.01.T01 | AT3G12500 | 57.851 | 242 | 101 | 1 | 35 | 276 | 86 | 326 | 3.61e-105 | 309 |
| MsG0780041426.01.T01 | AT3G16920 | 38.889 | 252 | 144 | 7 | 35 | 276 | 68 | 319 | 7.33e-51 | 170 |
| MsG0780041426.01.T01 | AT3G16920 | 38.889 | 252 | 144 | 7 | 35 | 276 | 92 | 343 | 9.64e-51 | 170 |
| MsG0780041426.01.T01 | AT1G05850 | 37.052 | 251 | 149 | 5 | 35 | 276 | 60 | 310 | 3.23e-50 | 168 |
| MsG0780041426.01.T01 | AT1G05850 | 37.052 | 251 | 149 | 5 | 35 | 276 | 60 | 310 | 3.23e-50 | 168 |
| MsG0780041426.01.T01 | AT3G54420 | 36.820 | 239 | 115 | 5 | 33 | 270 | 70 | 273 | 2.43e-49 | 164 |
| MsG0780041426.01.T01 | AT2G43590 | 37.131 | 237 | 108 | 7 | 35 | 270 | 68 | 264 | 7.26e-49 | 163 |
| MsG0780041426.01.T01 | AT2G43610 | 37.500 | 240 | 108 | 7 | 31 | 270 | 84 | 281 | 1.63e-42 | 147 |
| MsG0780041426.01.T01 | AT2G43580 | 34.599 | 237 | 114 | 7 | 35 | 270 | 69 | 265 | 7.54e-42 | 145 |
| MsG0780041426.01.T01 | AT2G43570 | 34.413 | 247 | 117 | 6 | 29 | 270 | 71 | 277 | 5.65e-41 | 143 |
| MsG0780041426.01.T01 | AT2G43620 | 35.833 | 240 | 112 | 7 | 31 | 270 | 86 | 283 | 1.28e-40 | 142 |
| MsG0780041426.01.T01 | AT1G56680 | 34.156 | 243 | 115 | 9 | 31 | 270 | 80 | 280 | 2.27e-30 | 115 |
| MsG0780041426.01.T01 | AT1G56680 | 34.156 | 243 | 115 | 9 | 31 | 270 | 103 | 303 | 4.91e-30 | 115 |
| MsG0780041426.01.T01 | AT2G43600 | 34.440 | 241 | 114 | 10 | 31 | 270 | 76 | 273 | 2.64e-28 | 109 |
| MsG0780041426.01.T01 | AT2G43600 | 34.440 | 241 | 114 | 10 | 31 | 270 | 97 | 294 | 4.63e-28 | 109 |
| MsG0780041426.01.T01 | AT3G47540 | 28.992 | 238 | 114 | 9 | 35 | 270 | 30 | 214 | 7.30e-27 | 104 |
| MsG0780041426.01.T01 | AT3G47540 | 28.139 | 231 | 111 | 9 | 35 | 263 | 30 | 207 | 7.58e-23 | 95.5 |
Find 65 sgRNAs with CRISPR-Local
Find 102 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTGGATACTGGACCTAATT+TGG | 0.146711 | 7:+90746584 | MsG0780041426.01.T01:CDS |
| CTTTCCTCCTCTGCATCTTT+AGG | 0.179427 | 7:+90745196 | MsG0780041426.01.T01:CDS |
| AGAGAAGAGATTGGTATAAT+AGG | 0.189791 | 7:-90745241 | None:intergenic |
| ACCAAATTGAGGAAAATATT+TGG | 0.190558 | 7:-90745362 | None:intergenic |
| AGCAATAAAGCAAAATAATA+TGG | 0.234582 | 7:-90745173 | None:intergenic |
| TTAGGAGTTGAATCATTTAT+TGG | 0.247252 | 7:+90745214 | MsG0780041426.01.T01:CDS |
| TGTGATGGTTGGAAAATATT+TGG | 0.256663 | 7:+90746413 | MsG0780041426.01.T01:CDS |
| TCAAAACTGCTCTATGGTTT+TGG | 0.260706 | 7:+90746355 | MsG0780041426.01.T01:CDS |
| GCTAATCGCACAGCTGGTTT+TGG | 0.280719 | 7:+90746456 | MsG0780041426.01.T01:CDS |
| TGATATGCACAATCCAAATT+AGG | 0.305058 | 7:-90746597 | None:intergenic |
| TTGTGTAGGAATTATAACTA+TGG | 0.344973 | 7:+90746258 | MsG0780041426.01.T01:intron |
| TAGGAGTTGAATCATTTATT+GGG | 0.345727 | 7:+90745215 | MsG0780041426.01.T01:CDS |
| AAAGATTGGCCTTGCTTTGA+AGG | 0.347412 | 7:+90745553 | MsG0780041426.01.T01:CDS |
| GAGAAGAGATTGGTATAATA+GGG | 0.356153 | 7:-90745240 | None:intergenic |
| TTGCTTTCAAAACTGCTCTA+TGG | 0.359827 | 7:+90746349 | MsG0780041426.01.T01:CDS |
| AAGCATAAGCCCCAAGAAAA+TGG | 0.365932 | 7:-90745484 | None:intergenic |
| GCAAGAGTTAATGATCGAAT+TGG | 0.385700 | 7:+90746531 | MsG0780041426.01.T01:CDS |
| AAGTTGTTCAATGTGGATAC+TGG | 0.403395 | 7:+90746573 | MsG0780041426.01.T01:CDS |
| GAATTGTTTGATACAATCTC+TGG | 0.409450 | 7:-90746321 | None:intergenic |
| TTTCCTCAATTTGGTACTAC+AGG | 0.418672 | 7:+90745370 | MsG0780041426.01.T01:CDS |
| GGGAAGGCGTTAGGATTTGA+TGG | 0.420884 | 7:+90746288 | MsG0780041426.01.T01:CDS |
| CCCCAAGAAAATGGTCCATC+AGG | 0.433362 | 7:-90745475 | None:intergenic |
| GCAATAAAGCAAAATAATAT+GGG | 0.436056 | 7:-90745172 | None:intergenic |
| GTATACCAAGATAGTTGAAT+AGG | 0.440524 | 7:-90745598 | None:intergenic |
| TCATTAACTCTTGCATCATC+AGG | 0.440723 | 7:-90746522 | None:intergenic |
| GTAGGAATATTGTGTCATAA+AGG | 0.444283 | 7:-90745276 | None:intergenic |
| GAGGTCCTATTCAACTATCT+TGG | 0.447585 | 7:+90745593 | MsG0780041426.01.T01:CDS |
| GGGTGGGCCACTGCACCTGA+TGG | 0.450425 | 7:+90745460 | MsG0780041426.01.T01:CDS |
| TTACTTATTAGAGAAGAGAT+TGG | 0.451038 | 7:-90745250 | None:intergenic |
| ACATTGTGACAAGAAGCTAT+TGG | 0.452503 | 7:-90746393 | None:intergenic |
| TGGAAGCTTCAATGAAGGAT+TGG | 0.458638 | 7:-90745342 | None:intergenic |
| GAATCACAGTAGTTACTTTG+AGG | 0.463755 | 7:-90745523 | None:intergenic |
| GGACCAGCAGGGAAGGCGTT+AGG | 0.472444 | 7:+90746279 | MsG0780041426.01.T01:CDS |
| AAATATAGTCAATGGTGGAT+TGG | 0.476185 | 7:+90746488 | MsG0780041426.01.T01:CDS |
| AATTATAACTATGGACCAGC+AGG | 0.477533 | 7:+90746267 | MsG0780041426.01.T01:CDS |
| AATCCTAACGCCTTCCCTGC+TGG | 0.489708 | 7:-90746282 | None:intergenic |
| GTGATTCTACTGACAAAGAT+TGG | 0.496296 | 7:+90745539 | MsG0780041426.01.T01:CDS |
| ATATTTGGAAGCTTCAATGA+AGG | 0.511487 | 7:-90745347 | None:intergenic |
| GCTTCAATGAAGGATTGGTA+AGG | 0.525028 | 7:-90745337 | None:intergenic |
| CAGATTTCACATGAAACTAC+TGG | 0.531637 | 7:+90745436 | MsG0780041426.01.T01:CDS |
| ATAGAAGCTAATCGCACAGC+TGG | 0.541134 | 7:+90746450 | MsG0780041426.01.T01:CDS |
| AGCTATCTCACGCTTTCTTG+TGG | 0.548914 | 7:-90745401 | None:intergenic |
| GGCAAAAGTTACAAAGGAAG+AGG | 0.557753 | 7:+90745574 | MsG0780041426.01.T01:CDS |
| TCTTGTCACAATGTGATGGT+TGG | 0.565818 | 7:+90746402 | MsG0780041426.01.T01:CDS |
| ACATGAAACTACTGGTGGGT+GGG | 0.569584 | 7:+90745444 | MsG0780041426.01.T01:CDS |
| AACTCCTAAAGATGCAGAGG+AGG | 0.579707 | 7:-90745200 | None:intergenic |
| GGAAGGCGTTAGGATTTGAT+GGG | 0.584389 | 7:+90746289 | MsG0780041426.01.T01:CDS |
| CAACCTGTAGTACCAAATTG+AGG | 0.584601 | 7:-90745373 | None:intergenic |
| GTCAATGGTGGATTGGAATG+TGG | 0.588138 | 7:+90746495 | MsG0780041426.01.T01:CDS |
| CAATAAAGCAAAATAATATG+GGG | 0.588254 | 7:-90745171 | None:intergenic |
| TTTGAAGGCAAAAGTTACAA+AGG | 0.591242 | 7:+90745568 | MsG0780041426.01.T01:CDS |
| ATGCAGTGTCATCTTTGTGT+AGG | 0.594953 | 7:-90745294 | None:intergenic |
| CACATGAAACTACTGGTGGG+TGG | 0.597128 | 7:+90745443 | MsG0780041426.01.T01:CDS |
| TAACTATGGACCAGCAGGGA+AGG | 0.603344 | 7:+90746272 | MsG0780041426.01.T01:CDS |
| CTTCAATGAAGGATTGGTAA+GGG | 0.607116 | 7:-90745336 | None:intergenic |
| AAATGGTCCATCAGGTGCAG+TGG | 0.617726 | 7:-90745467 | None:intergenic |
| GTGACAAATATAGTCAATGG+TGG | 0.618848 | 7:+90746483 | MsG0780041426.01.T01:CDS |
| TTGGTGACAAATATAGTCAA+TGG | 0.622222 | 7:+90746480 | MsG0780041426.01.T01:CDS |
| TTCAACTCCTAAAGATGCAG+AGG | 0.624622 | 7:-90745203 | None:intergenic |
| AGCTTCTTGTCACAATGTGA+TGG | 0.626977 | 7:+90746398 | MsG0780041426.01.T01:CDS |
| ATTTCACATGAAACTACTGG+TGG | 0.650794 | 7:+90745439 | MsG0780041426.01.T01:CDS |
| AATAAAGCAAAATAATATGG+GGG | 0.669386 | 7:-90745170 | None:intergenic |
| ATTATAACTATGGACCAGCA+GGG | 0.676909 | 7:+90746268 | MsG0780041426.01.T01:CDS |
| ATATACTAAGTTGTTCAATG+TGG | 0.678623 | 7:+90746566 | MsG0780041426.01.T01:CDS |
| TTTCACATGAAACTACTGGT+GGG | 0.698219 | 7:+90745440 | MsG0780041426.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATACGAAATAATTACTAAAT+CGG | - | Chr7:90745852-90745871 | None:intergenic | 15.0% |
| !! | TTTGTCAACAAAAAAAAAAA+AGG | + | Chr7:90746023-90746042 | MsG0780041426.01.T01:intron | 15.0% |
| !! | AATAAAGCAAAATAATATGG+GGG | - | Chr7:90745173-90745192 | None:intergenic | 20.0% |
| !! | AGCAATAAAGCAAAATAATA+TGG | - | Chr7:90745176-90745195 | None:intergenic | 20.0% |
| !! | CAATAAAGCAAAATAATATG+GGG | - | Chr7:90745174-90745193 | None:intergenic | 20.0% |
| !! | GCAATAAAGCAAAATAATAT+GGG | - | Chr7:90745175-90745194 | None:intergenic | 20.0% |
| !! | TACGAAATAATTACTAAATC+GGG | - | Chr7:90745851-90745870 | None:intergenic | 20.0% |
| !! | TGATTAAGTATAATATGGTT+GGG | - | Chr7:90746084-90746103 | None:intergenic | 20.0% |
| !! | TTGATTAAGTATAATATGGT+TGG | - | Chr7:90746085-90746104 | None:intergenic | 20.0% |
| ! | ACCAAATTGAGGAAAATATT+TGG | - | Chr7:90745365-90745384 | None:intergenic | 25.0% |
| ! | ATATACTAAGTTGTTCAATG+TGG | + | Chr7:90746566-90746585 | MsG0780041426.01.T01:CDS | 25.0% |
| ! | TAGGAGTTGAATCATTTATT+GGG | + | Chr7:90745215-90745234 | MsG0780041426.01.T01:CDS | 25.0% |
| ! | TCGCTTGATTAAGTATAATA+TGG | - | Chr7:90746089-90746108 | None:intergenic | 25.0% |
| ! | TTACTTATTAGAGAAGAGAT+TGG | - | Chr7:90745253-90745272 | None:intergenic | 25.0% |
| ! | TTAGGAGTTGAATCATTTAT+TGG | + | Chr7:90745214-90745233 | MsG0780041426.01.T01:CDS | 25.0% |
| ! | TTGTGTAGGAATTATAACTA+TGG | + | Chr7:90746258-90746277 | MsG0780041426.01.T01:intron | 25.0% |
| !! | TCCAAATATTTTCCTCAATT+TGG | + | Chr7:90745361-90745380 | MsG0780041426.01.T01:CDS | 25.0% |
| !!! | CATAGAAACAATTTTAGGTT+TGG | - | Chr7:90745704-90745723 | None:intergenic | 25.0% |
| AGAGAAGAGATTGGTATAAT+AGG | - | Chr7:90745244-90745263 | None:intergenic | 30.0% | |
| AGCTAATGAGTTTCAGTTAA+AGG | + | Chr7:90746121-90746140 | MsG0780041426.01.T01:intron | 30.0% | |
| ATATTTGGAAGCTTCAATGA+AGG | - | Chr7:90745350-90745369 | None:intergenic | 30.0% | |
| CTACACAAAAGTTTATGAGA+GGG | - | Chr7:90746246-90746265 | None:intergenic | 30.0% | |
| GAATTGTTTGATACAATCTC+TGG | - | Chr7:90746324-90746343 | None:intergenic | 30.0% | |
| GAGAAGAGATTGGTATAATA+GGG | - | Chr7:90745243-90745262 | None:intergenic | 30.0% | |
| GTAGGAATATTGTGTCATAA+AGG | - | Chr7:90745279-90745298 | None:intergenic | 30.0% | |
| GTATACCAAGATAGTTGAAT+AGG | - | Chr7:90745601-90745620 | None:intergenic | 30.0% | |
| TACACAAAAGTTTATGAGAG+GGG | - | Chr7:90746245-90746264 | None:intergenic | 30.0% | |
| TGATATGCACAATCCAAATT+AGG | - | Chr7:90746600-90746619 | None:intergenic | 30.0% | |
| TGTGATGGTTGGAAAATATT+TGG | + | Chr7:90746413-90746432 | MsG0780041426.01.T01:CDS | 30.0% | |
| TTTGAAGGCAAAAGTTACAA+AGG | + | Chr7:90745568-90745587 | MsG0780041426.01.T01:CDS | 30.0% | |
| ! | AGGATCATATGGATTAAAAC+TGG | - | Chr7:90745782-90745801 | None:intergenic | 30.0% |
| ! | GAGCTCATAGAAACAATTTT+AGG | - | Chr7:90745709-90745728 | None:intergenic | 30.0% |
| ! | TTGGTAAGGGTAAAAGTTTT+TGG | - | Chr7:90745326-90745345 | None:intergenic | 30.0% |
| ! | TTGGTGACAAATATAGTCAA+TGG | + | Chr7:90746480-90746499 | MsG0780041426.01.T01:CDS | 30.0% |
| ! | TTTGAAACTAATTTAGCGAC+CGG | - | Chr7:90745895-90745914 | None:intergenic | 30.0% |
| !! | AAATATAGTCAATGGTGGAT+TGG | + | Chr7:90746488-90746507 | MsG0780041426.01.T01:CDS | 30.0% |
| AAAACTTATTTCCCAAGCTG+AGG | - | Chr7:90745807-90745826 | None:intergenic | 35.0% | |
| AAATAATATGGGGGTGAGAA+TGG | - | Chr7:90745164-90745183 | None:intergenic | 35.0% | |
| AAGTTGTTCAATGTGGATAC+TGG | + | Chr7:90746573-90746592 | MsG0780041426.01.T01:CDS | 35.0% | |
| AATTATAACTATGGACCAGC+AGG | + | Chr7:90746267-90746286 | MsG0780041426.01.T01:CDS | 35.0% | |
| ACATTGTGACAAGAAGCTAT+TGG | - | Chr7:90746396-90746415 | None:intergenic | 35.0% | |
| AGCAGCGTTAAAATTCTTGA+CGG | + | Chr7:90745756-90745775 | MsG0780041426.01.T01:intron | 35.0% | |
| ATTATAACTATGGACCAGCA+GGG | + | Chr7:90746268-90746287 | MsG0780041426.01.T01:CDS | 35.0% | |
| ATTTCACATGAAACTACTGG+TGG | + | Chr7:90745439-90745458 | MsG0780041426.01.T01:CDS | 35.0% | |
| CAGATTTCACATGAAACTAC+TGG | + | Chr7:90745436-90745455 | MsG0780041426.01.T01:CDS | 35.0% | |
| CCTACACAAAAGTTTATGAG+AGG | - | Chr7:90746247-90746266 | None:intergenic | 35.0% | |
| GCAAGAGTTAATGATCGAAT+TGG | + | Chr7:90746531-90746550 | MsG0780041426.01.T01:CDS | 35.0% | |
| GTGACAAATATAGTCAATGG+TGG | + | Chr7:90746483-90746502 | MsG0780041426.01.T01:CDS | 35.0% | |
| GTGATTCTACTGACAAAGAT+TGG | + | Chr7:90745539-90745558 | MsG0780041426.01.T01:CDS | 35.0% | |
| TATTTCGTATCTGTAGTGAC+CGG | + | Chr7:90745862-90745881 | MsG0780041426.01.T01:intron | 35.0% | |
| TCATTAACTCTTGCATCATC+AGG | - | Chr7:90746525-90746544 | None:intergenic | 35.0% | |
| TGTGGATTTGGACAAAGAAT+TGG | - | Chr7:90745678-90745697 | None:intergenic | 35.0% | |
| TTGCTTTCAAAACTGCTCTA+TGG | + | Chr7:90746349-90746368 | MsG0780041426.01.T01:CDS | 35.0% | |
| TTTCACATGAAACTACTGGT+GGG | + | Chr7:90745440-90745459 | MsG0780041426.01.T01:CDS | 35.0% | |
| TTTCCTCAATTTGGTACTAC+AGG | + | Chr7:90745370-90745389 | MsG0780041426.01.T01:CDS | 35.0% | |
| ! | CCTCTCATAAACTTTTGTGT+AGG | + | Chr7:90746244-90746263 | MsG0780041426.01.T01:intron | 35.0% |
| ! | CTTCAATGAAGGATTGGTAA+GGG | - | Chr7:90745339-90745358 | None:intergenic | 35.0% |
| ! | GAATCACAGTAGTTACTTTG+AGG | - | Chr7:90745526-90745545 | None:intergenic | 35.0% |
| ! | TAACTTTTGCCTTCAAAGCA+AGG | - | Chr7:90745565-90745584 | None:intergenic | 35.0% |
| ! | TCAAAACTGCTCTATGGTTT+TGG | + | Chr7:90746355-90746374 | MsG0780041426.01.T01:CDS | 35.0% |
| !!! | CAATTTTAGGTTTGGATGTG+TGG | - | Chr7:90745696-90745715 | None:intergenic | 35.0% |
| !!! | TAAGGGTAAAAGTTTTTGGC+AGG | - | Chr7:90745322-90745341 | None:intergenic | 35.0% |
| AAGCATAAGCCCCAAGAAAA+TGG | - | Chr7:90745487-90745506 | None:intergenic | 40.0% | |
| AGCTTCTTGTCACAATGTGA+TGG | + | Chr7:90746398-90746417 | MsG0780041426.01.T01:CDS | 40.0% | |
| ATGCAGTGTCATCTTTGTGT+AGG | - | Chr7:90745297-90745316 | None:intergenic | 40.0% | |
| CAACCTGTAGTACCAAATTG+AGG | - | Chr7:90745376-90745395 | None:intergenic | 40.0% | |
| GAGGTCCTATTCAACTATCT+TGG | + | Chr7:90745593-90745612 | MsG0780041426.01.T01:CDS | 40.0% | |
| GGCAAAAGTTACAAAGGAAG+AGG | + | Chr7:90745574-90745593 | MsG0780041426.01.T01:CDS | 40.0% | |
| TAGGTTTGGATGTGTGGATT+TGG | - | Chr7:90745690-90745709 | None:intergenic | 40.0% | |
| TCTTGTCACAATGTGATGGT+TGG | + | Chr7:90746402-90746421 | MsG0780041426.01.T01:CDS | 40.0% | |
| TGGAAGCTTCAATGAAGGAT+TGG | - | Chr7:90745345-90745364 | None:intergenic | 40.0% | |
| TGTGGATACTGGACCTAATT+TGG | + | Chr7:90746584-90746603 | MsG0780041426.01.T01:CDS | 40.0% | |
| TTCAACTCCTAAAGATGCAG+AGG | - | Chr7:90745206-90745225 | None:intergenic | 40.0% | |
| TTTAGCGACCGGTTATAAAC+CGG | - | Chr7:90745884-90745903 | None:intergenic | 40.0% | |
| ! | GCTTCAATGAAGGATTGGTA+AGG | - | Chr7:90745340-90745359 | None:intergenic | 40.0% |
| ! | TGTAGTGACCGGTTTATAAC+CGG | + | Chr7:90745873-90745892 | MsG0780041426.01.T01:intron | 40.0% |
| !! | AAAGATTGGCCTTGCTTTGA+AGG | + | Chr7:90745553-90745572 | MsG0780041426.01.T01:CDS | 40.0% |
| !! | ACCTGATGGACCATTTTCTT+GGG | + | Chr7:90745474-90745493 | MsG0780041426.01.T01:CDS | 40.0% |
| AACTCCTAAAGATGCAGAGG+AGG | - | Chr7:90745203-90745222 | None:intergenic | 45.0% | |
| AAGCTGAGGTGAGGATCATA+TGG | - | Chr7:90745793-90745812 | None:intergenic | 45.0% | |
| AGCTATCTCACGCTTTCTTG+TGG | - | Chr7:90745404-90745423 | None:intergenic | 45.0% | |
| ATAGAAGCTAATCGCACAGC+TGG | + | Chr7:90746450-90746469 | MsG0780041426.01.T01:CDS | 45.0% | |
| ATATGATCCTCACCTCAGCT+TGG | + | Chr7:90745792-90745811 | MsG0780041426.01.T01:intron | 45.0% | |
| CTTTCCTCCTCTGCATCTTT+AGG | + | Chr7:90745196-90745215 | MsG0780041426.01.T01:CDS | 45.0% | |
| TATGATCCTCACCTCAGCTT+GGG | + | Chr7:90745793-90745812 | MsG0780041426.01.T01:intron | 45.0% | |
| TTATTTCCCAAGCTGAGGTG+AGG | - | Chr7:90745802-90745821 | None:intergenic | 45.0% | |
| ! | ACATGAAACTACTGGTGGGT+GGG | + | Chr7:90745444-90745463 | MsG0780041426.01.T01:CDS | 45.0% |
| !! | CACCTGATGGACCATTTTCT+TGG | + | Chr7:90745473-90745492 | MsG0780041426.01.T01:CDS | 45.0% |
| !! | CCTGATGGACCATTTTCTTG+GGG | + | Chr7:90745475-90745494 | MsG0780041426.01.T01:CDS | 45.0% |
| !! | GGAAGGCGTTAGGATTTGAT+GGG | + | Chr7:90746289-90746308 | MsG0780041426.01.T01:CDS | 45.0% |
| !! | GTCAATGGTGGATTGGAATG+TGG | + | Chr7:90746495-90746514 | MsG0780041426.01.T01:CDS | 45.0% |
| !! | TATTAGATTAAATTATTATT+TGG | + | Chr7:90745645-90745664 | MsG0780041426.01.T01:intron | 5.0% |
| AAATGGTCCATCAGGTGCAG+TGG | - | Chr7:90745470-90745489 | None:intergenic | 50.0% | |
| CACATGAAACTACTGGTGGG+TGG | + | Chr7:90745443-90745462 | MsG0780041426.01.T01:CDS | 50.0% | |
| CCCCAAGAAAATGGTCCATC+AGG | - | Chr7:90745478-90745497 | None:intergenic | 50.0% | |
| TAACTATGGACCAGCAGGGA+AGG | + | Chr7:90746272-90746291 | MsG0780041426.01.T01:CDS | 50.0% | |
| !! | GCTAATCGCACAGCTGGTTT+TGG | + | Chr7:90746456-90746475 | MsG0780041426.01.T01:CDS | 50.0% |
| !! | GGGAAGGCGTTAGGATTTGA+TGG | + | Chr7:90746288-90746307 | MsG0780041426.01.T01:CDS | 50.0% |
| !!! | TCGCACAGCTGGTTTTGGAT+TGG | + | Chr7:90746461-90746480 | MsG0780041426.01.T01:CDS | 50.0% |
| AATCCTAACGCCTTCCCTGC+TGG | - | Chr7:90746285-90746304 | None:intergenic | 55.0% | |
| !! | GGACCAGCAGGGAAGGCGTT+AGG | + | Chr7:90746279-90746298 | MsG0780041426.01.T01:CDS | 65.0% |
| GGGTGGGCCACTGCACCTGA+TGG | + | Chr7:90745460-90745479 | MsG0780041426.01.T01:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 90745151 | 90746632 | 90745151 | ID=MsG0780041426.01;Name=MsG0780041426.01 |
| Chr7 | mRNA | 90745151 | 90746632 | 90745151 | ID=MsG0780041426.01.T01;Parent=MsG0780041426.01;Name=MsG0780041426.01.T01;_AED=0.29;_eAED=0.30;_QI=0|0|0|1|1|1|2|0|276 |
| Chr7 | exon | 90745151 | 90745614 | 90745151 | ID=MsG0780041426.01.T01:exon:3825;Parent=MsG0780041426.01.T01 |
| Chr7 | exon | 90746266 | 90746632 | 90746266 | ID=MsG0780041426.01.T01:exon:3826;Parent=MsG0780041426.01.T01 |
| Chr7 | CDS | 90745151 | 90745614 | 90745151 | ID=MsG0780041426.01.T01:cds;Parent=MsG0780041426.01.T01 |
| Chr7 | CDS | 90746266 | 90746632 | 90746266 | ID=MsG0780041426.01.T01:cds;Parent=MsG0780041426.01.T01 |
| Gene Sequence |
| Protein sequence |
