AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0250410

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0250410 MtrunA17_Chr2g0308321 96.584 322 11 0 1 322 218 539 0.0 639
Msa0250410 MtrunA17_Chr4g0061651 61.846 325 97 6 1 322 170 470 9.53e-120 352
Msa0250410 MtrunA17_Chr5g0420051 41.176 136 70 3 1 135 228 354 6.26e-17 80.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0250410 AT1G69170.1 50.000 70 34 1 1 70 171 239 5.52e-14 72.4
Msa0250410 AT5G50570.1 37.241 145 73 7 1 137 148 282 9.28e-12 65.5
Msa0250410 AT5G50570.2 37.241 145 73 7 1 137 148 282 9.28e-12 65.5
Msa0250410 AT5G50670.1 37.241 145 73 7 1 137 148 282 9.28e-12 65.5
Msa0250410 AT5G50670.2 37.241 145 73 7 1 137 148 282 9.28e-12 65.5

Find 101 sgRNAs with CRISPR-Local

Find 196 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAGATTACATTCACTAAAT+TGG 0.174095 2_2:+39122294 None:intergenic
TTTCCTTTCCTTCAAGAAAA+TGG 0.189331 2_2:-39123625 Msa0250410:CDS
TCGATTGGGTTGAAGAATTC+TGG 0.204342 2_2:+39123392 None:intergenic
TGTATCAATGTGTGACATTC+AGG 0.241788 2_2:-39122367 Msa0250410:three_prime_UTR
TGAGGATGTGCATTTATAAT+TGG 0.243639 2_2:+39123685 None:intergenic
CCACAAGTTGGTATTCATTC+TGG 0.255651 2_2:-39125315 Msa0250410:CDS
ACTATCCAGGTTTCATTTGC+TGG 0.261393 2_2:-39125406 None:intergenic
AGCTTCAGGTCTTTAAATAC+TGG 0.310334 2_2:+39122150 None:intergenic
GGGAGGTTGCTTCAGTCATA+TGG 0.315390 2_2:-39125285 Msa0250410:CDS
GCATCAGAGGCGAGCCTTGC+AGG 0.317553 2_2:-39123077 Msa0250410:CDS
TTACTGTTTGATATCAATAT+AGG 0.332199 2_2:+39123235 None:intergenic
TATTGTTGTACGAAGGAAAC+AGG 0.337560 2_2:+39123653 None:intergenic
GCTTTCTACGACGCTCATTA+TGG 0.341005 2_2:+39125337 None:intergenic
TTGTCATAAACACTTCAATA+AGG 0.351623 2_2:-39122341 Msa0250410:three_prime_UTR
GGAGGTTGCTTCAGTCATAT+GGG 0.364253 2_2:-39125284 Msa0250410:CDS
ATCCAAGGAGGCTCAAATTC+CGG 0.366080 2_2:-39123547 Msa0250410:CDS
CGATTGGGTTGAAGAATTCT+GGG 0.380885 2_2:+39123393 None:intergenic
TCAGGAAAGAACTTAATATC+TGG 0.386887 2_2:-39122542 Msa0250410:three_prime_UTR
AAACATGACTCATACTATAA+TGG 0.390275 2_2:+39123323 None:intergenic
CGCTTCCTAAGGAGGTATCT+TGG 0.402978 2_2:+39123509 None:intergenic
TGACTAGAAATATTCGTATC+AGG 0.405865 2_2:-39122579 Msa0250410:three_prime_UTR
AGCTGTCGTAAACGTCTTGC+AGG 0.408574 2_2:-39125360 Msa0250410:CDS
TGAAGCTGACCAATGGCGGC+TGG 0.414356 2_2:-39122464 Msa0250410:three_prime_UTR
CCTAAAACCGGTTCCGCCTT+CGG 0.416837 2_2:+39123723 None:intergenic
TTACATTACATTCTAGCTGT+TGG 0.416888 2_2:+39122410 None:intergenic
ATAAAAGCCGAAGGCGGAAC+CGG 0.423047 2_2:-39123730 Msa0250410:CDS
GATTCTGCAGAAGAAGCTTC+AGG 0.423244 2_2:+39122136 None:intergenic
GCATGGGAATTCCTGTCGAT+TGG 0.429542 2_2:+39123377 None:intergenic
TTACGTAAAATGCTTTCAAC+AGG 0.432048 2_2:-39123018 Msa0250410:intron
ATCCGGAATTTGAGCCTCCT+TGG 0.442331 2_2:+39123545 None:intergenic
ATCAGCTGTTCAAGGATTAT+CGG 0.450349 2_2:-39123461 Msa0250410:CDS
GGTATTCATTCTGGAAGAGC+TGG 0.460050 2_2:-39125306 Msa0250410:CDS
TTATTCCAAGATACCTCCTT+AGG 0.463781 2_2:-39123514 Msa0250410:CDS
CATGGGAATTCCTGTCGATT+GGG 0.467279 2_2:+39123378 None:intergenic
GAAACAGAATCAGCTGTTCA+AGG 0.477684 2_2:-39123469 Msa0250410:CDS
ATGAGTCATGTTTCTGAAAG+TGG 0.480897 2_2:-39123313 Msa0250410:CDS
AGAAATGTGGAAATAGTAAC+TGG 0.480981 2_2:-39123762 Msa0250410:CDS
AATGGTCGTGCCATCTTCAC+AGG 0.486457 2_2:+39123138 None:intergenic
TATTGCGTCCCAGCCGCCAT+TGG 0.487032 2_2:+39122455 None:intergenic
GACCGTCTTTCCTGTGAAGA+TGG 0.490853 2_2:-39123148 Msa0250410:CDS
CTGAAAGTGGCGTCTCGGAT+AGG 0.491794 2_2:-39123300 Msa0250410:CDS
TCAGCTGTTCAAGGATTATC+GGG 0.494261 2_2:-39123460 Msa0250410:CDS
ATTGAAGTCTTCGCTTCCTA+AGG 0.501723 2_2:+39123498 None:intergenic
CTGATGTTAGCATGGTTCCT+TGG 0.502694 2_2:+39123833 None:intergenic
CCTCACCGGGGAGTATCTCT+TGG 0.503402 2_2:+39123797 None:intergenic
CAGGATAAGAACTTAGTGTC+AGG 0.506236 2_2:-39122560 Msa0250410:three_prime_UTR
AATGGTGCTACATCTGCTAC+TGG 0.508879 2_2:-39123607 Msa0250410:CDS
ATAATTGGAATAGAAGAAAG+AGG 0.509513 2_2:+39123700 None:intergenic
ATGATTCTTCCTGCTCTCTA+CGG 0.510890 2_2:-39123042 Msa0250410:CDS
GACTCATACTATAATGGCTA+TGG 0.516959 2_2:+39123329 None:intergenic
GAATTTGAGCCTCCTTGGAT+AGG 0.517666 2_2:+39123550 None:intergenic
TTACGTAATCCGTAGAGAGC+AGG 0.520716 2_2:+39123033 None:intergenic
TTGCTGGCTGAATTTGATGA+TGG 0.522089 2_2:-39125390 Msa0250410:CDS
AGAAGAAAGAGGCCTAAAAC+CGG 0.523177 2_2:+39123711 None:intergenic
CTATAATGGCTATGGCTGCT+CGG 0.525746 2_2:+39123337 None:intergenic
TTGGAATAATGATTGTGATC+CGG 0.528558 2_2:+39123528 None:intergenic
CGACAGTTGAAGCAAGTCAA+TGG 0.529505 2_2:+39123120 None:intergenic
AATAACTAAATGACCAGGCA+TGG 0.532105 2_2:+39123360 None:intergenic
TCGACAGGAATTCCCATGCC+TGG 0.536060 2_2:-39123373 Msa0250410:CDS
TTTGAGCCTCCTTGGATAGG+AGG 0.541499 2_2:+39123553 None:intergenic
TCAGAAGATAAGACCTCACC+GGG 0.542752 2_2:+39123784 None:intergenic
GAGGTTGCTTCAGTCATATG+GGG 0.543950 2_2:-39125283 Msa0250410:CDS
CTAGTCACAACAAGCTGCAC+AGG 0.544970 2_2:+39122595 None:intergenic
AATTCTTCAACCCAATCGAC+AGG 0.554625 2_2:-39123388 Msa0250410:CDS
CGTTGACAAAATCCGACCCC+TGG 0.557627 2_2:+39123176 None:intergenic
GTATTCATTCTGGAAGAGCT+GGG 0.561734 2_2:-39125305 Msa0250410:CDS
GAAGCTGACCAATGGCGGCT+GGG 0.561893 2_2:-39122463 Msa0250410:three_prime_UTR
TCTGAGTTAAATCATACAGA+TGG 0.562489 2_2:-39123271 Msa0250410:CDS
GAAGTCTTCGCTTCCTAAGG+AGG 0.563138 2_2:+39123501 None:intergenic
TGTTTCTGAAAGTGGCGTCT+CGG 0.565635 2_2:-39123305 Msa0250410:CDS
AATTGTTTATTGTTGTACGA+AGG 0.567910 2_2:+39123646 None:intergenic
TTGGATATATGTAGATAGAG+AGG 0.569382 2_2:+39122429 None:intergenic
CTCAGAAGATAAGACCTCAC+CGG 0.577587 2_2:+39123783 None:intergenic
ATATGCCAAGAGATACTCCC+CGG 0.578163 2_2:-39123802 Msa0250410:CDS
CTCGGAATAACTAAATGACC+AGG 0.578215 2_2:+39123355 None:intergenic
GGAAACAGGGTTCTTGAATG+AGG 0.578570 2_2:+39123667 None:intergenic
CTTACAGATAGCAGATTCCA+AGG 0.587504 2_2:-39123850 Msa0250410:intron
TGCCATCTTCACAGGAAAGA+CGG 0.587669 2_2:+39123146 None:intergenic
TCAAGGATTATCGGGAGTAT+CGG 0.590961 2_2:-39123452 Msa0250410:CDS
TCTGTATGATTTAACTCAGA+CGG 0.594329 2_2:+39123274 None:intergenic
GGTCAATATCCTCCTATCCA+AGG 0.597117 2_2:-39123562 Msa0250410:CDS
AAAAGATGCTGATGTTAGCA+TGG 0.597201 2_2:+39123825 None:intergenic
TTCAGCGTGTTGAGCATCAG+AGG 0.601537 2_2:-39123090 Msa0250410:CDS
CGGCTCGTGAAGCTGACCAA+TGG 0.603959 2_2:-39122471 Msa0250410:three_prime_UTR
CGTCGTAGAAAGCCACAAGT+TGG 0.607871 2_2:-39125327 Msa0250410:CDS
TGGATATATGTAGATAGAGA+GGG 0.609573 2_2:+39122430 None:intergenic
CCAGAATGAATACCAACTTG+TGG 0.611115 2_2:+39125315 None:intergenic
ATAACTAAATGACCAGGCAT+GGG 0.613150 2_2:+39123361 None:intergenic
CTCGTGAAGCTGACCAATGG+CGG 0.613637 2_2:-39122468 Msa0250410:three_prime_UTR
GTCTTATCTTCTGAGAAATG+TGG 0.623862 2_2:-39123775 Msa0250410:CDS
AGCATCTTCTGTGAAAACAA+TGG 0.648314 2_2:-39123583 Msa0250410:CDS
TGCAGACCTATAAAAGCCGA+AGG 0.654610 2_2:-39123739 Msa0250410:CDS
ATTGTTGTACGAAGGAAACA+GGG 0.661617 2_2:+39123654 None:intergenic
GGATTATCGGGAGTATCGGA+CGG 0.671634 2_2:-39123448 Msa0250410:CDS
CCAAGAGATACTCCCCGGTG+AGG 0.681967 2_2:-39123797 Msa0250410:CDS
CAATATCCTCCTATCCAAGG+AGG 0.704515 2_2:-39123559 Msa0250410:CDS
TTCATTCTGGAAGAGCTGGG+AGG 0.705305 2_2:-39125302 Msa0250410:CDS
AGACCTATAAAAGCCGAAGG+CGG 0.722914 2_2:-39123736 Msa0250410:CDS
AGGTTGCTTCAGTCATATGG+GGG 0.727655 2_2:-39125282 Msa0250410:intron
TTATCGGGAGTATCGGACGG+TGG 0.747831 2_2:-39123445 Msa0250410:CDS
CAGAAGATAAGACCTCACCG+GGG 0.863209 2_2:+39123785 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAACAAAAAGATTCATTTTA+TGG - chr2_2:39123140-39123159 Msa0250410:CDS 15.0%
!! GCTTTATAATATTAATAATC+AGG + chr2_2:39123535-39123554 None:intergenic 15.0%
!!! AAAAAAAAAGTCATTTTGAA+AGG + chr2_2:39123557-39123576 None:intergenic 15.0%
!! TTTAATATTCAAAACCATAT+TGG - chr2_2:39123588-39123607 Msa0250410:CDS 15.0%
!! GAAATGAAAATTTATTTATG+TGG - chr2_2:39124820-39124839 Msa0250410:intron 15.0%
!! AGTTGAAAATTAGAGTATAA+TGG - chr2_2:39122576-39122595 Msa0250410:three_prime_UTR 20.0%
!! TTAAGAACAAAGTGAAAATT+GGG + chr2_2:39122691-39122710 None:intergenic 20.0%
!! ATTAAGAACAAAGTGAAAAT+TGG + chr2_2:39122692-39122711 None:intergenic 20.0%
!! AAGAGAAAATTTGTAACAAA+TGG + chr2_2:39122963-39122982 None:intergenic 20.0%
!! TTAATAATCAGGAAAGTAAA+TGG + chr2_2:39123524-39123543 None:intergenic 20.0%
!! TTACTGTTTGATATCAATAT+AGG + chr2_2:39124306-39124325 None:intergenic 20.0%
!! TAATTTGAAAACATGAAACA+TGG + chr2_2:39124708-39124727 None:intergenic 20.0%
!! TTAGATTACATTCACTAAAT+TGG + chr2_2:39125247-39125266 None:intergenic 20.0%
! AAATCCACTAAAAAGTAGTT+AGG + chr2_2:39122337-39122356 None:intergenic 25.0%
! TAAATATCGAGCTTGAAATT+TGG - chr2_2:39122470-39122489 Msa0250410:three_prime_UTR 25.0%
! AAATATCGAGCTTGAAATTT+GGG - chr2_2:39122471-39122490 Msa0250410:three_prime_UTR 25.0%
! AGATAATGATATCATCTACT+TGG - chr2_2:39122625-39122644 Msa0250410:intron 25.0%
!! TTTACAATTTTCCACAAAGA+TGG - chr2_2:39122756-39122775 Msa0250410:intron 25.0%
! AAACTGAATTACGAATACTT+TGG + chr2_2:39122798-39122817 None:intergenic 25.0%
!! TTGAATTCTGATTGATTCTA+TGG - chr2_2:39122908-39122927 Msa0250410:intron 25.0%
! AAATTATACTAGAGAGTTTG+AGG - chr2_2:39123352-39123371 Msa0250410:CDS 25.0%
! ATTCAAAACCATATTGGTAA+AGG - chr2_2:39123594-39123613 Msa0250410:CDS 25.0%
! TATGAAAACCTTTACCAATA+TGG + chr2_2:39123605-39123624 None:intergenic 25.0%
! ATAATTGGAATAGAAGAAAG+AGG + chr2_2:39123841-39123860 None:intergenic 25.0%
! AATTGTTTATTGTTGTACGA+AGG + chr2_2:39123895-39123914 None:intergenic 25.0%
! AAACATGACTCATACTATAA+TGG + chr2_2:39124218-39124237 None:intergenic 25.0%
!!! GAAGAATAAATCTTTGAAGT+CGG - chr2_2:39124764-39124783 Msa0250410:intron 25.0%
! AAATGTTGTGAGTGATTTAA+TGG + chr2_2:39124883-39124902 None:intergenic 25.0%
! TTGTCATAAACACTTCAATA+AGG - chr2_2:39125197-39125216 Msa0250410:intron 25.0%
!!! ATTTTGATGAATGTTGATTC+TGG - chr2_2:39125286-39125305 Msa0250410:CDS 25.0%
CACTAAAAAGTAGTTAGGAA+GGG + chr2_2:39122332-39122351 None:intergenic 30.0%
GATGATATCATTATCTGTGA+AGG + chr2_2:39122621-39122640 None:intergenic 30.0%
ACAAAAGTGGGAGAATAATT+CGG + chr2_2:39122992-39123011 None:intergenic 30.0%
!! AATTATTCTCCCACTTTTGT+TGG - chr2_2:39122992-39123011 Msa0250410:intron 30.0%
AGATGACAAATGATAAAGGA+TGG + chr2_2:39123025-39123044 None:intergenic 30.0%
CCAAAGATGACAAATGATAA+AGG + chr2_2:39123029-39123048 None:intergenic 30.0%
CCTTTATCATTTGTCATCTT+TGG - chr2_2:39123026-39123045 Msa0250410:exon 30.0%
!! GTGTAAAGCTAGCTTTAATT+TGG - chr2_2:39123048-39123067 Msa0250410:CDS 30.0%
ATTTGGATTCTTTAGTCACA+TGG - chr2_2:39123065-39123084 Msa0250410:CDS 30.0%
ATTGTTGAACAAGTCACTAA+TGG + chr2_2:39123101-39123120 None:intergenic 30.0%
! GACTTGTTCAACAATTACAA+TGG - chr2_2:39123106-39123125 Msa0250410:CDS 30.0%
GGTTTAGTGACTATCATAAT+CGG - chr2_2:39123161-39123180 Msa0250410:CDS 30.0%
! AATGCCAAATTTTCGTCAAT+GGG - chr2_2:39123193-39123212 Msa0250410:CDS 30.0%
TCAAATAATCCAATGCAAAC+AGG - chr2_2:39123463-39123482 Msa0250410:CDS 30.0%
TAATTACTCAGAGACAAACA+TGG + chr2_2:39123652-39123671 None:intergenic 30.0%
GAGTAATTAACAAGATTGTG+TGG - chr2_2:39123663-39123682 Msa0250410:CDS 30.0%
AGAAATGTGGAAATAGTAAC+TGG - chr2_2:39123776-39123795 Msa0250410:CDS 30.0%
TGAGGATGTGCATTTATAAT+TGG + chr2_2:39123856-39123875 None:intergenic 30.0%
TTTCCTTTCCTTCAAGAAAA+TGG - chr2_2:39123913-39123932 Msa0250410:intron 30.0%
TTGGAATAATGATTGTGATC+CGG + chr2_2:39124013-39124032 None:intergenic 30.0%
TCTGTATGATTTAACTCAGA+CGG + chr2_2:39124267-39124286 None:intergenic 30.0%
TCTGAGTTAAATCATACAGA+TGG - chr2_2:39124267-39124286 Msa0250410:intron 30.0%
! TGAAATCGCACATTTTCAAA+TGG - chr2_2:39124326-39124345 Msa0250410:intron 30.0%
! CACATTTTCAAATGGTAGAT+GGG - chr2_2:39124334-39124353 Msa0250410:intron 30.0%
! ACATTTTCAAATGGTAGATG+GGG - chr2_2:39124335-39124354 Msa0250410:intron 30.0%
TTACGTAAAATGCTTTCAAC+AGG - chr2_2:39124520-39124539 Msa0250410:intron 30.0%
! GAAGTTCTTTTCTGCAAAAA+TGG + chr2_2:39124921-39124940 None:intergenic 30.0%
TGACTAGAAATATTCGTATC+AGG - chr2_2:39124959-39124978 Msa0250410:intron 30.0%
TCAGGAAAGAACTTAATATC+TGG - chr2_2:39124996-39125015 Msa0250410:intron 30.0%
!! TAGTTTTACTTGTGCTGAAT+CGG - chr2_2:39125047-39125066 Msa0250410:intron 30.0%
TGGATATATGTAGATAGAGA+GGG + chr2_2:39125111-39125130 None:intergenic 30.0%
TTGGATATATGTAGATAGAG+AGG + chr2_2:39125112-39125131 None:intergenic 30.0%
! TTACATTACATTCTAGCTGT+TGG + chr2_2:39125131-39125150 None:intergenic 30.0%
CATATGGGGGTAATATTCTA+TGG - chr2_2:39122269-39122288 Msa0250410:three_prime_UTR 35.0%
TAAAAAGTAGTTAGGAAGGG+TGG + chr2_2:39122329-39122348 None:intergenic 35.0%
CCACTAAAAAGTAGTTAGGA+AGG + chr2_2:39122333-39122352 None:intergenic 35.0%
!!! CCTTCCTAACTACTTTTTAG+TGG - chr2_2:39122330-39122349 Msa0250410:three_prime_UTR 35.0%
!! CAATGGGCTAATTAATTGCA+TGG + chr2_2:39122383-39122402 None:intergenic 35.0%
CATGCAATTAATTAGCCCAT+TGG - chr2_2:39122381-39122400 Msa0250410:three_prime_UTR 35.0%
AACATCTGATCTTGATCCAA+CGG + chr2_2:39122410-39122429 None:intergenic 35.0%
!!! ACATCTTTTTTCCCTTCATC+GGG + chr2_2:39122880-39122899 None:intergenic 35.0%
!!! CACATCTTTTTTCCCTTCAT+CGG + chr2_2:39122881-39122900 None:intergenic 35.0%
ATGGATATAGATCTACAGCA+TGG - chr2_2:39122927-39122946 Msa0250410:intron 35.0%
! GAATGCCAAATTTTCGTCAA+TGG - chr2_2:39123192-39123211 Msa0250410:CDS 35.0%
TAATTAGCTGTTCGTCCAAA+TGG + chr2_2:39123221-39123240 None:intergenic 35.0%
AAAAGATGCTGATGTTAGCA+TGG + chr2_2:39123716-39123735 None:intergenic 35.0%
! GTCTTATCTTCTGAGAAATG+TGG - chr2_2:39123763-39123782 Msa0250410:CDS 35.0%
ATTGTTGTACGAAGGAAACA+GGG + chr2_2:39123887-39123906 None:intergenic 35.0%
TATTGTTGTACGAAGGAAAC+AGG + chr2_2:39123888-39123907 None:intergenic 35.0%
! ATGTAGCACCATTTTCTTGA+AGG + chr2_2:39123924-39123943 None:intergenic 35.0%
AGCATCTTCTGTGAAAACAA+TGG - chr2_2:39123955-39123974 Msa0250410:intron 35.0%
TTATTCCAAGATACCTCCTT+AGG - chr2_2:39124024-39124043 Msa0250410:intron 35.0%
ATCAGCTGTTCAAGGATTAT+CGG - chr2_2:39124077-39124096 Msa0250410:intron 35.0%
ATAACTAAATGACCAGGCAT+GGG + chr2_2:39124180-39124199 None:intergenic 35.0%
AATAACTAAATGACCAGGCA+TGG + chr2_2:39124181-39124200 None:intergenic 35.0%
GACTCATACTATAATGGCTA+TGG + chr2_2:39124212-39124231 None:intergenic 35.0%
! ATGAGTCATGTTTCTGAAAG+TGG - chr2_2:39124225-39124244 Msa0250410:intron 35.0%
! GCACATTTTCAAATGGTAGA+TGG - chr2_2:39124333-39124352 Msa0250410:intron 35.0%
! TCACCAAATTTTCTCTACGT+CGG - chr2_2:39124572-39124591 Msa0250410:intron 35.0%
AAATACACAGTAGTACGACA+TGG + chr2_2:39124620-39124639 None:intergenic 35.0%
TGTATCAATGTGTGACATTC+AGG - chr2_2:39125171-39125190 Msa0250410:intron 35.0%
AGCTTCAGGTCTTTAAATAC+TGG + chr2_2:39125391-39125410 None:intergenic 35.0%
! TTGCTGGCTGAATTTGATGA+TGG - chr2_2:39122148-39122167 Msa0250410:three_prime_UTR 40.0%
CCAGAATGAATACCAACTTG+TGG + chr2_2:39122226-39122245 None:intergenic 40.0%
!! CCACAAGTTGGTATTCATTC+TGG - chr2_2:39122223-39122242 Msa0250410:three_prime_UTR 40.0%
! GTATTCATTCTGGAAGAGCT+GGG - chr2_2:39122233-39122252 Msa0250410:three_prime_UTR 40.0%
GCAATTAATTAGCCCATTGG+TGG - chr2_2:39122384-39122403 Msa0250410:three_prime_UTR 40.0%
CTTCAGCAAATATATGCACG+TGG + chr2_2:39122558-39122577 None:intergenic 40.0%
TGATAGGCATTCCATCTTTG+TGG + chr2_2:39122770-39122789 None:intergenic 40.0%
AAGATGGAATGCCTATCATC+TGG - chr2_2:39122772-39122791 Msa0250410:intron 40.0%
GAATACTTTGGCCAGATGAT+AGG + chr2_2:39122786-39122805 None:intergenic 40.0%
!!! CATCTTTTTTCCCTTCATCG+GGG + chr2_2:39122879-39122898 None:intergenic 40.0%
GGTGCAAAACCAACAAAAGT+GGG + chr2_2:39123004-39123023 None:intergenic 40.0%
TGGTGCAAAACCAACAAAAG+TGG + chr2_2:39123005-39123024 None:intergenic 40.0%
TGGATTCTTTAGTCACATGG+TGG - chr2_2:39123068-39123087 Msa0250410:CDS 40.0%
GGTTCCCATTGACGAAAATT+TGG + chr2_2:39123200-39123219 None:intergenic 40.0%
TTTCGTCAATGGGAACCATT+TGG - chr2_2:39123203-39123222 Msa0250410:CDS 40.0%
AGACTGCTTGGAATAGCTTA+TGG + chr2_2:39123416-39123435 None:intergenic 40.0%
CTTACAGATAGCAGATTCCA+AGG - chr2_2:39123688-39123707 Msa0250410:CDS 40.0%
AGAAGAAAGAGGCCTAAAAC+CGG + chr2_2:39123830-39123849 None:intergenic 40.0%
! GCACCATTTTCTTGAAGGAA+AGG + chr2_2:39123919-39123938 None:intergenic 40.0%
ATTGAAGTCTTCGCTTCCTA+AGG + chr2_2:39124043-39124062 None:intergenic 40.0%
GAAACAGAATCAGCTGTTCA+AGG - chr2_2:39124069-39124088 Msa0250410:intron 40.0%
TCAGCTGTTCAAGGATTATC+GGG - chr2_2:39124078-39124097 Msa0250410:intron 40.0%
! TCAAGGATTATCGGGAGTAT+CGG - chr2_2:39124086-39124105 Msa0250410:intron 40.0%
!! CGATTGGGTTGAAGAATTCT+GGG + chr2_2:39124148-39124167 None:intergenic 40.0%
!! TCGATTGGGTTGAAGAATTC+TGG + chr2_2:39124149-39124168 None:intergenic 40.0%
AATTCTTCAACCCAATCGAC+AGG - chr2_2:39124150-39124169 Msa0250410:intron 40.0%
CTCGGAATAACTAAATGACC+AGG + chr2_2:39124186-39124205 None:intergenic 40.0%
ATGATTCTTCCTGCTCTCTA+CGG - chr2_2:39124496-39124515 Msa0250410:intron 40.0%
TAGCCGACGTAGAGAAAATT+TGG + chr2_2:39124578-39124597 None:intergenic 40.0%
CAGGATAAGAACTTAGTGTC+AGG - chr2_2:39124978-39124997 Msa0250410:intron 40.0%
GCTTTCTACGACGCTCATTA+TGG + chr2_2:39122204-39122223 None:intergenic 45.0%
! GGTATTCATTCTGGAAGAGC+TGG - chr2_2:39122232-39122251 Msa0250410:three_prime_UTR 45.0%
GGAGGTTGCTTCAGTCATAT+GGG - chr2_2:39122254-39122273 Msa0250410:three_prime_UTR 45.0%
GAGGTTGCTTCAGTCATATG+GGG - chr2_2:39122255-39122274 Msa0250410:three_prime_UTR 45.0%
AGGTTGCTTCAGTCATATGG+GGG - chr2_2:39122256-39122275 Msa0250410:three_prime_UTR 45.0%
GGAAGGGTGGAAAATTGAGA+AGG + chr2_2:39122316-39122335 None:intergenic 45.0%
! GACCACCTATGTATTGATCG+CGG - chr2_2:39122514-39122533 Msa0250410:three_prime_UTR 45.0%
TTCCGCGATCAATACATAGG+TGG + chr2_2:39122519-39122538 None:intergenic 45.0%
GCATTCCGCGATCAATACAT+AGG + chr2_2:39122522-39122541 None:intergenic 45.0%
TTAGAACCTGTGAGACTGCT+TGG + chr2_2:39123428-39123447 None:intergenic 45.0%
GCTTTACAGCCTGTTTGCAT+TGG + chr2_2:39123475-39123494 None:intergenic 45.0%
CTGATGTTAGCATGGTTCCT+TGG + chr2_2:39123708-39123727 None:intergenic 45.0%
ATATGCCAAGAGATACTCCC+CGG - chr2_2:39123736-39123755 Msa0250410:CDS 45.0%
TCAGAAGATAAGACCTCACC+GGG + chr2_2:39123757-39123776 None:intergenic 45.0%
CTCAGAAGATAAGACCTCAC+CGG + chr2_2:39123758-39123777 None:intergenic 45.0%
TGCAGACCTATAAAAGCCGA+AGG - chr2_2:39123799-39123818 Msa0250410:CDS 45.0%
AGACCTATAAAAGCCGAAGG+CGG - chr2_2:39123802-39123821 Msa0250410:CDS 45.0%
GGAAACAGGGTTCTTGAATG+AGG + chr2_2:39123874-39123893 None:intergenic 45.0%
!! AATGGTGCTACATCTGCTAC+TGG - chr2_2:39123931-39123950 Msa0250410:intron 45.0%
GGTCAATATCCTCCTATCCA+AGG - chr2_2:39123976-39123995 Msa0250410:intron 45.0%
CAATATCCTCCTATCCAAGG+AGG - chr2_2:39123979-39123998 Msa0250410:intron 45.0%
GAATTTGAGCCTCCTTGGAT+AGG + chr2_2:39123991-39124010 None:intergenic 45.0%
ATCCAAGGAGGCTCAAATTC+CGG - chr2_2:39123991-39124010 Msa0250410:intron 45.0%
! CATGGGAATTCCTGTCGATT+GGG + chr2_2:39124163-39124182 None:intergenic 45.0%
CTATAATGGCTATGGCTGCT+CGG + chr2_2:39124204-39124223 None:intergenic 45.0%
! TGTTTCTGAAAGTGGCGTCT+CGG - chr2_2:39124233-39124252 Msa0250410:intron 45.0%
! AATGGTAGATGGGGTTTTCC+AGG - chr2_2:39124344-39124363 Msa0250410:intron 45.0%
! ATGGTAGATGGGGTTTTCCA+GGG - chr2_2:39124345-39124364 Msa0250410:intron 45.0%
TGCCATCTTCACAGGAAAGA+CGG + chr2_2:39124395-39124414 None:intergenic 45.0%
CGACAGTTGAAGCAAGTCAA+TGG + chr2_2:39124421-39124440 None:intergenic 45.0%
! ATTTTCTCGCTTCACCTGCA+AGG + chr2_2:39124478-39124497 None:intergenic 45.0%
TTACGTAATCCGTAGAGAGC+AGG + chr2_2:39124508-39124527 None:intergenic 45.0%
!! TTTGAATATTAAATAAAAAA+TGG + chr2_2:39123581-39123600 None:intergenic 5.0%
AGCTGTCGTAAACGTCTTGC+AGG - chr2_2:39122178-39122197 Msa0250410:three_prime_UTR 50.0%
CGTCGTAGAAAGCCACAAGT+TGG - chr2_2:39122211-39122230 Msa0250410:three_prime_UTR 50.0%
! TTCATTCTGGAAGAGCTGGG+AGG - chr2_2:39122236-39122255 Msa0250410:three_prime_UTR 50.0%
GGGAGGTTGCTTCAGTCATA+TGG - chr2_2:39122253-39122272 Msa0250410:three_prime_UTR 50.0%
TTGATCCAACGGCCACCAAT+GGG + chr2_2:39122399-39122418 None:intergenic 50.0%
! TGTATTGATCGCGGAATGCG+TGG - chr2_2:39122523-39122542 Msa0250410:three_prime_UTR 50.0%
! GTATTGATCGCGGAATGCGT+GGG - chr2_2:39122524-39122543 Msa0250410:three_prime_UTR 50.0%
ACGTGTTCTTACCCCGATGA+AGG - chr2_2:39122865-39122884 Msa0250410:intron 50.0%
CGTGTTCTTACCCCGATGAA+GGG - chr2_2:39122866-39122885 Msa0250410:intron 50.0%
GCTATTCCAAGCAGTCTCAC+AGG - chr2_2:39123419-39123438 Msa0250410:CDS 50.0%
CAGAAGATAAGACCTCACCG+GGG + chr2_2:39123756-39123775 None:intergenic 50.0%
! GTTCCGCCTTCGGCTTTTAT+AGG + chr2_2:39123808-39123827 None:intergenic 50.0%
ATAAAAGCCGAAGGCGGAAC+CGG - chr2_2:39123808-39123827 Msa0250410:CDS 50.0%
TTTGAGCCTCCTTGGATAGG+AGG + chr2_2:39123988-39124007 None:intergenic 50.0%
ATCCGGAATTTGAGCCTCCT+TGG + chr2_2:39123996-39124015 None:intergenic 50.0%
!! CGCTTCCTAAGGAGGTATCT+TGG + chr2_2:39124032-39124051 None:intergenic 50.0%
GAAGTCTTCGCTTCCTAAGG+AGG + chr2_2:39124040-39124059 None:intergenic 50.0%
!! GGATTATCGGGAGTATCGGA+CGG - chr2_2:39124090-39124109 Msa0250410:intron 50.0%
GCATGGGAATTCCTGTCGAT+TGG + chr2_2:39124164-39124183 None:intergenic 50.0%
! TGGTAGATGGGGTTTTCCAG+GGG - chr2_2:39124346-39124365 Msa0250410:intron 50.0%
GACCGTCTTTCCTGTGAAGA+TGG - chr2_2:39124390-39124409 Msa0250410:intron 50.0%
AATGGTCGTGCCATCTTCAC+AGG + chr2_2:39124403-39124422 None:intergenic 50.0%
TTCAGCGTGTTGAGCATCAG+AGG - chr2_2:39124448-39124467 Msa0250410:intron 50.0%
CTAGTCACAACAAGCTGCAC+AGG + chr2_2:39124946-39124965 None:intergenic 50.0%
! ATTAGCCCATTGGTGGCCGT+TGG - chr2_2:39122391-39122410 Msa0250410:three_prime_UTR 55.0%
CTTGATCCAACGGCCACCAA+TGG + chr2_2:39122400-39122419 None:intergenic 55.0%
! CCTAAAACCGGTTCCGCCTT+CGG + chr2_2:39123818-39123837 None:intergenic 55.0%
!! TTATCGGGAGTATCGGACGG+TGG - chr2_2:39124093-39124112 Msa0250410:intron 55.0%
TCGACAGGAATTCCCATGCC+TGG - chr2_2:39124165-39124184 Msa0250410:intron 55.0%
CTGAAAGTGGCGTCTCGGAT+AGG - chr2_2:39124238-39124257 Msa0250410:intron 55.0%
! AGATGGGGTTTTCCAGGGGT+CGG - chr2_2:39124350-39124369 Msa0250410:intron 55.0%
CGTTGACAAAATCCGACCCC+TGG + chr2_2:39124365-39124384 None:intergenic 55.0%
CTCGTGAAGCTGACCAATGG+CGG - chr2_2:39125070-39125089 Msa0250410:intron 55.0%
!! CCTCACCGGGGAGTATCTCT+TGG + chr2_2:39123744-39123763 None:intergenic 60.0%
CCAAGAGATACTCCCCGGTG+AGG - chr2_2:39123741-39123760 Msa0250410:CDS 60.0%
!! CCGAAGGCGGAACCGGTTTT+AGG - chr2_2:39123815-39123834 Msa0250410:CDS 60.0%
CGGCTCGTGAAGCTGACCAA+TGG - chr2_2:39125067-39125086 Msa0250410:intron 60.0%
TGAAGCTGACCAATGGCGGC+TGG - chr2_2:39125074-39125093 Msa0250410:intron 60.0%
GAAGCTGACCAATGGCGGCT+GGG - chr2_2:39125075-39125094 Msa0250410:intron 60.0%
TATTGCGTCCCAGCCGCCAT+TGG + chr2_2:39125086-39125105 None:intergenic 60.0%
GCATCAGAGGCGAGCCTTGC+AGG - chr2_2:39124461-39124480 Msa0250410:intron 65.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 39122142 39125418 39122142 ID=Msa0250410;Name=Msa0250410
chr2_2 mRNA 39122142 39125418 39122142 ID=Msa0250410-mRNA-1;Parent=Msa0250410;Name=Msa0250410-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|1|0.66|1|0.5|0|3|503|322
chr2_2 exon 39122142 39122630 39122142 ID=Msa0250410-mRNA-1:exon:12152;Parent=Msa0250410-mRNA-1
chr2_2 exon 39123019 39123865 39123019 ID=Msa0250410-mRNA-1:exon:12151;Parent=Msa0250410-mRNA-1
chr2_2 exon 39125283 39125418 39125283 ID=Msa0250410-mRNA-1:exon:12150;Parent=Msa0250410-mRNA-1
chr2_2 CDS 39125283 39125418 39125283 ID=Msa0250410-mRNA-1:cds;Parent=Msa0250410-mRNA-1
chr2_2 CDS 39123033 39123865 39123033 ID=Msa0250410-mRNA-1:cds;Parent=Msa0250410-mRNA-1
chr2_2 three_prime_UTR 39123019 39123032 39123019 ID=Msa0250410-mRNA-1:three_prime_utr;Parent=Msa0250410-mRNA-1
chr2_2 three_prime_UTR 39122142 39122630 39122142 ID=Msa0250410-mRNA-1:three_prime_utr;Parent=Msa0250410-mRNA-1
Gene Sequence

>Msa0250410

TTTCATTTGCTGGCTGAATTTGATGATGGTAAGCGTAGCTGTCGTAAACGTCTTGCAGGCCATAATGAGCGTCGTAGAAAGCCACAAGTTGGTATTCATTCTGGAAGAGCTGGGAGGTTGCTTCAGTCATATGGGGATAGCAGATTCCAAGGAACCATGCTAACATCAGCATCTTTTATATGCCAAGAGATACTCCCCGGTGAGGTCTTATCTTCTGAGAAATGTGGAAATAGTAACTGGTGCAGACCTATAAAAGCCGAAGGCGGAACCGGTTTTAGGCCTCTTTCTTCTATTCCAATTATAAATGCACATCCTCATTCAAGAACCCTGTTTCCTTCGTACAACAATAAACAATTTCCTTTCCTTCAAGAAAATGGTGCTACATCTGCTACTGGAAGCATCTTCTGTGAAAACAATGGTCAATATCCTCCTATCCAAGGAGGCTCAAATTCCGGATCACAATCATTATTCCAAGATACCTCCTTAGGAAGCGAAGACTTCAATGTTTTTGAAACAGAATCAGCTGTTCAAGGATTATCGGGAGTATCGGACGGTGGTTGTGCTCTCTCTCTTCTGTCATCTCAATCCCAGAATTCTTCAACCCAATCGACAGGAATTCCCATGCCTGGTCATTTAGTTATTCCGAGCAGCCATAGCCATTATAGTATGAGTCATGTTTCTGAAAGTGGCGTCTCGGATAGGTTTCCGTCTGAGTTAAATCATACAGATGGAAGTCATCTTAGTCCTATATTGATATCAAACAGTAATGAAATCGCACATTTTCAAATGGTAGATGGGGTTTTCCAGGGGTCGGATTTTGTCAACGTGAAAGACCGTCTTTCCTGTGAAGATGGCACGACCATTGACTTGCTTCAACTGTCGTCACAACTTCAGCGTGTTGAGCATCAGAGGCGAGCCTTGCAGGTGAAGCGAGAAAATGATTCTTCCTGCTCTCTACGGATTACGTAA
Protein sequence

>Msa0250410

FHLLAEFDDGKRSCRKRLAGHNERRRKPQVGIHSGRAGRLLQSYGDSRFQGTMLTSASFICQEILPGEVLSSEKCGNSNWCRPIKAEGGTGFRPLSSIPIINAHPHSRTLFPSYNNKQFPFLQENGATSATGSIFCENNGQYPPIQGGSNSGSQSLFQDTSLGSEDFNVFETESAVQGLSGVSDGGCALSLLSSQSQNSSTQSTGIPMPGHLVIPSSHSHYSMSHVSESGVSDRFPSELNHTDGSHLSPILISNSNEIAHFQMVDGVFQGSDFVNVKDRLSCEDGTTIDLLQLSSQLQRVEHQRRALQVKRENDSSCSLRIT*