AlfalfaGEDB | Msa0548060

M. sativa cultivar zhongmu-4 / Msa0548060

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0548060	PNY02171.1	52.542	118	48	2	1	112	50	165	1.83e-26	115
Msa0548060	PNY02171.1	57.143	70	29	1	108	177	258	326	1.96e-11	74.3

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0548060	A0A2K3NGK5	52.542	118	48	2	1	112	50	165	8.72e-27	115
Msa0548060	A0A2K3NGK5	57.143	70	29	1	108	177	258	326	9.35e-12	74.3

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0068630	Msa0548060	0.831628	1.498537e-55	-8.615850e-47
Msa0118050	Msa0548060	0.833086	6.527393e-56	-8.615850e-47
Msa0118060	Msa0548060	0.814184	1.752797e-51	-8.615850e-47
Msa0145750	Msa0548060	0.825109	5.607351e-54	-8.615850e-47
Msa0153200	Msa0548060	0.813350	2.676075e-51	-8.615850e-47
Msa0162580	Msa0548060	0.814246	1.698975e-51	-8.615850e-47
Msa0232850	Msa0548060	0.829269	5.658685e-55	-8.615850e-47
Msa0267000	Msa0548060	0.821132	4.751053e-53	-8.615850e-47
Msa0267010	Msa0548060	0.827723	1.336481e-54	-8.615850e-47
Msa0342240	Msa0548060	0.801606	8.317648e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0548060	MtrunA17_Chr7g0255021	36.559	93	57	1	13	105	76	166	5.59e-15	73.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 45 sgRNAs with CRISPR-Local

Find 208 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGGAGGCCATTGTAAGGG+AGG	0.650330	4_1:-41765790	Msa0548060:CDS
AAATGTCATCATGTTAAAGA+TGG	0.492131	4_1:-41765826	Msa0548060:CDS
AATGGGTTCTAGTAGTGGTG+TGG	0.581573	4_1:-41766561	Msa0548060:CDS
AATTAAAAGCCCAGTATCAT+CGG	0.552075	4_1:-41766274	Msa0548060:CDS
AATTGTCTGATTTCCTTCTT+TGG	0.270940	4_1:+41766293	None:intergenic
ACACCACTACTAGAACCCAT+TGG	0.625021	4_1:+41766563	None:intergenic
ACCATTCAACCGATGATACT+GGG	0.533581	4_1:+41766265	None:intergenic
ACTCTCTACTCAAATTGGAT+TGG	0.492596	4_1:-41766516	Msa0548060:CDS
AGTAATCATCATGATTTCCA+AGG	0.555546	4_1:+41764862	None:intergenic
ATAATGTGGATGATTGTGTG+TGG	0.650338	4_1:-41764743	Msa0548060:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATTGTCTGATTTCCTTCTT+TGG	+	chr4_1:41765004-41765023	None:intergenic	30.0%
AATTAAAAGCCCAGTATCAT+CGG	-	chr4_1:41765020-41765039	Msa0548060:intron	30.0%
TACTCAAAGATGAACCAAAA+TGG	-	chr4_1:41765205-41765224	Msa0548060:intron	30.0%
AAAGATGAACCAAAATGGAA+GGG	-	chr4_1:41765210-41765229	Msa0548060:intron	30.0%
CTGAACAATCAATTGGTATT+TGG	+	chr4_1:41765315-41765334	None:intergenic	30.0%
TCATAGTCTGAACAATCAAT+TGG	+	chr4_1:41765322-41765341	None:intergenic	30.0%
TGGGACAAAAAGTAGTAAAA+AGG	-	chr4_1:41765368-41765387	Msa0548060:intron	30.0%
AATCATATTAGGCAGAAGTT+AGG	-	chr4_1:41765637-41765656	Msa0548060:intron	30.0%
ACTATGCGCAACAAAATATA+AGG	-	chr4_1:41766272-41766291	Msa0548060:CDS	30.0%
CTATGCGCAACAAAATATAA+GGG	-	chr4_1:41766273-41766292	Msa0548060:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_1	gene	41764734	41766582	41764734	ID=Msa0548060;Name=Msa0548060
chr4_1	mRNA	41764734	41766582	41764734	ID=Msa0548060-mRNA-1;Parent=Msa0548060;Name=Msa0548060-mRNA-1;_AED=0.49;_eAED=0.49;_QI=0\|0\|0\|0.33\|1\|1\|3\|0\|227
chr4_1	exon	41766264	41766582	41766264	ID=Msa0548060-mRNA-1:exon:10733;Parent=Msa0548060-mRNA-1
chr4_1	exon	41765769	41765981	41765769	ID=Msa0548060-mRNA-1:exon:10732;Parent=Msa0548060-mRNA-1
chr4_1	exon	41764734	41764885	41764734	ID=Msa0548060-mRNA-1:exon:10731;Parent=Msa0548060-mRNA-1
chr4_1	CDS	41766264	41766582	41766264	ID=Msa0548060-mRNA-1:cds;Parent=Msa0548060-mRNA-1
chr4_1	CDS	41765769	41765981	41765769	ID=Msa0548060-mRNA-1:cds;Parent=Msa0548060-mRNA-1
chr4_1	CDS	41764734	41764885	41764734	ID=Msa0548060-mRNA-1:cds;Parent=Msa0548060-mRNA-1

Gene Sequence

>Msa0548060

ATGGGTTCTAGTAGTGGTGTGGATTCCTTTAATGACATGCCACAACTCTCTACTCAAATTGGATTGGAAAATATCATGCTCGATGAAGAACAAAATTCTACTAAGAAAAAAACTCAAACTCGTGCGAGTTTTTCACAAGAAGAAGACACACTTTTATTTCAATCACAACTCAACATTTTGAAAGACTTAATTGTGGAAGTCGACCAAAAATCATATGGTTTTTGGAATAGAGTCAGAGACAAACATAACATCTACCACGACCAACTTCCAAAGAAGGAAATCAGACAATTAAAAGCCCAGTATCATCGGTTGAATGGTCACTATGACCAAGCATCACCATTGACCCGTCCAATGGGACAAAAAGTAGTAAAAAGGAAGATTAAAGGAAAAGCAGTTGCAACGTCTTCTAATAATATTAATTTAGCTGTCAAGAAAAAAGTTGCAAGAGAAAGAAATGTCATCATGTTAAAGATGGACGAAGCAAGGGAAAAGGAGGCCATTGTAAGGGAGGCAGATGCAAAGGCAAGGGAGGCCCTTGGAAATCATGATGATTACTTCCAAACAAGGGTTGATGCAGTTCGTAGATTAGGCCTTTCACCATTGCAGAAGTGCATTGTCGCTCTTCGTTTGTTAGCATATGGAGTTCCTGTTGATAATGTGGATGATTGTGTGTGGAGTGTTTAG

Protein sequence

>Msa0548060

MGSSSGVDSFNDMPQLSTQIGLENIMLDEEQNSTKKKTQTRASFSQEEDTLLFQSQLNILKDLIVEVDQKSYGFWNRVRDKHNIYHDQLPKKEIRQLKAQYHRLNGHYDQASPLTRPMGQKVVKRKIKGKAVATSSNNINLAVKKKVARERNVIMLKMDEAREKEAIVREADAKAREALGNHDDYFQTRVDAVRRLGLSPLQKCIVALRLLAYGVPVDNVDDCVWSV*