AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0636540


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0636540 MtrunA17_Chr4g0019351 78.788 99 16 2 1 97 1 96 2.43e-48 149
Msa0636540 MtrunA17_Chr4g0019341 77.778 99 17 2 1 97 1 96 5.49e-46 142
Msa0636540 MtrunA17_Chr4g0019361 56.122 98 18 2 1 97 1 74 1.32e-25 90.9
Msa0636540 MtrunA17_Chr4g0018981 45.361 97 34 2 1 97 1 78 6.92e-20 76.3
Msa0636540 MtrunA17_Chr4g0018971 46.875 96 30 2 1 96 1 75 1.05e-18 73.2
Msa0636540 MtrunA17_Chr4g0019021 38.739 111 48 4 1 97 1 105 1.02e-16 68.9
Msa0636540 MtrunA17_Chr8g0368881 37.755 98 46 2 1 97 1 84 1.28e-16 68.2
Msa0636540 MtrunA17_Chr4g0018961 42.268 97 34 2 1 97 1 75 1.01e-14 63.2
Msa0636540 MtrunA17_Chr4g0018931 36.735 98 41 2 1 97 1 78 6.41e-12 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 25 sgRNAs with CRISPR-Local

Find 114 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGAAGTGCTCTCTTATTT+TGG 0.217478 4_3:-27195606 Msa0636540:CDS
TTGTGGCCACGTGCTTTATC+AGG 0.279751 4_3:+27195337 None:intergenic
CGTTAGGTTGGATCAATTTA+CGG 0.318370 4_3:-27194844 Msa0636540:three_prime_UTR
TGTATAGTTTCAGATAAAAT+AGG 0.333321 4_3:-27195394 Msa0636540:intron
AATAAGAAGAGACTCCTTGC+TGG 0.444927 4_3:-27195367 Msa0636540:CDS
CTATGAATGATATGGGCGTT+AGG 0.451730 4_3:-27194860 Msa0636540:exon
GAATGATATGGGCGTTAGGT+TGG 0.467597 4_3:-27194856 Msa0636540:three_prime_UTR
GACTCCTTGCAGAAAATTGT+GGG 0.487595 4_3:-27194931 Msa0636540:CDS
GATCAATTTACGGATGAAGT+TGG 0.531820 4_3:-27194834 Msa0636540:three_prime_UTR
AGACTCCTTGCAGAAAATTG+TGG 0.531866 4_3:-27194932 Msa0636540:CDS
AAGTAAAACCTGCAAAATTG+TGG 0.541805 4_3:+27195320 None:intergenic
CGAAAGCTCGAGTGTTGCAC+GGG 0.550887 4_3:-27194741 Msa0636540:three_prime_UTR
TTGCAGAAAATTGTGGGAGT+AGG 0.564611 4_3:-27194925 Msa0636540:CDS
ACACTCGAGCTTTCGACCTA+GGG 0.574814 4_3:+27194749 None:intergenic
TCGAAAGCTCGAGTGTTGCA+CGG 0.581217 4_3:-27194742 Msa0636540:three_prime_UTR
AACACTCGAGCTTTCGACCT+AGG 0.585121 4_3:+27194748 None:intergenic
TACATGGTCCTCATGCTGAT+CGG 0.594268 4_3:-27194901 Msa0636540:CDS
TTTCATCAAAGACATTAGCA+AGG 0.595257 4_3:-27195556 Msa0636540:CDS
AATTGTGGGAGTAGGATACA+TGG 0.612508 4_3:-27194917 Msa0636540:CDS
ATATCATTCATAGAAAATTG+TGG 0.617375 4_3:+27194870 None:intergenic
AGAAGAGACTCCTTGCTGGT+AGG 0.629830 4_3:-27195363 Msa0636540:CDS
TATCAGGCTTCCTACCAGCA+AGG 0.630061 4_3:+27195353 None:intergenic
CAACGTGGCCGATCAGCATG+AGG 0.638536 4_3:+27194893 None:intergenic
CATAGAAAATTGTGGCAACG+TGG 0.674818 4_3:+27194878 None:intergenic
AGGAAGCCTGATAAAGCACG+TGG 0.728123 4_3:-27195343 Msa0636540:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTCTTGTATTAATATT+TGG - chr4_3:27195321-27195340 Msa0636540:intron 10.0%
!!! TTTTTTCTTGTATTAATATT+TGG - chr4_3:27195321-27195340 Msa0636540:intron 10.0%
!! AATAACAAAATGGATATTAT+GGG + chr4_3:27194832-27194851 None:intergenic 15.0%
!! TATTATTTATGGATATTATG+GGG + chr4_3:27195024-27195043 None:intergenic 15.0%
!! GTATTATTTATGGATATTAT+GGG + chr4_3:27195025-27195044 None:intergenic 15.0%
!! AGTATTATTTATGGATATTA+TGG + chr4_3:27195026-27195045 None:intergenic 15.0%
!!! TATTTTGAGAAAAGAAAAAT+GGG - chr4_3:27195260-27195279 Msa0636540:intron 15.0%
!! TTGGTAGAAAAAATAAATAA+TGG - chr4_3:27195503-27195522 Msa0636540:intron 15.0%
!! TGGTAGAAAAAATAAATAAT+GGG - chr4_3:27195504-27195523 Msa0636540:intron 15.0%
!! AATAACAAAATGGATATTAT+GGG + chr4_3:27194832-27194851 None:intergenic 15.0%
!! TATTATTTATGGATATTATG+GGG + chr4_3:27195024-27195043 None:intergenic 15.0%
!! GTATTATTTATGGATATTAT+GGG + chr4_3:27195025-27195044 None:intergenic 15.0%
!! AGTATTATTTATGGATATTA+TGG + chr4_3:27195026-27195045 None:intergenic 15.0%
!!! TATTTTGAGAAAAGAAAAAT+GGG - chr4_3:27195260-27195279 Msa0636540:intron 15.0%
!! TTGGTAGAAAAAATAAATAA+TGG - chr4_3:27195503-27195522 Msa0636540:intron 15.0%
!! TGGTAGAAAAAATAAATAAT+GGG - chr4_3:27195504-27195523 Msa0636540:intron 15.0%
!! ATAACAAAATGGATATTATG+GGG + chr4_3:27194831-27194850 None:intergenic 20.0%
!! CAATAACAAAATGGATATTA+TGG + chr4_3:27194833-27194852 None:intergenic 20.0%
!! TGTATAGTTTCAGATAAAAT+AGG - chr4_3:27194924-27194943 Msa0636540:CDS 20.0%
!! TGGATAAAGTTCAATAAATA+AGG - chr4_3:27195184-27195203 Msa0636540:intron 20.0%
!!! CTATTTTGAGAAAAGAAAAA+TGG - chr4_3:27195259-27195278 Msa0636540:intron 20.0%
!! ATATCATTCATAGAAAATTG+TGG + chr4_3:27195451-27195470 None:intergenic 20.0%
!!! ACAATTTTCTATGAATGATA+TGG - chr4_3:27195450-27195469 Msa0636540:intron 20.0%
!!! CAATTTTCTATGAATGATAT+GGG - chr4_3:27195451-27195470 Msa0636540:intron 20.0%
!! ATAACAAAATGGATATTATG+GGG + chr4_3:27194831-27194850 None:intergenic 20.0%
!! CAATAACAAAATGGATATTA+TGG + chr4_3:27194833-27194852 None:intergenic 20.0%
!! TGTATAGTTTCAGATAAAAT+AGG - chr4_3:27194924-27194943 Msa0636540:CDS 20.0%
!! TGGATAAAGTTCAATAAATA+AGG - chr4_3:27195184-27195203 Msa0636540:intron 20.0%
!!! CTATTTTGAGAAAAGAAAAA+TGG - chr4_3:27195259-27195278 Msa0636540:intron 20.0%
!! ATATCATTCATAGAAAATTG+TGG + chr4_3:27195451-27195470 None:intergenic 20.0%
!!! ACAATTTTCTATGAATGATA+TGG - chr4_3:27195450-27195469 Msa0636540:intron 20.0%
!!! CAATTTTCTATGAATGATAT+GGG - chr4_3:27195451-27195470 Msa0636540:intron 20.0%
! AAACGACTATTATTTAGAGA+CGG + chr4_3:27195053-27195072 None:intergenic 25.0%
! CTCTAAATAATAGTCGTTTA+AGG - chr4_3:27195054-27195073 Msa0636540:intron 25.0%
! CCACTAACTACAATTAATAA+GGG + chr4_3:27195153-27195172 None:intergenic 25.0%
! TCCACTAACTACAATTAATA+AGG + chr4_3:27195154-27195173 None:intergenic 25.0%
! AAAGAAACAATTATTGTGAG+CGG - chr4_3:27195284-27195303 Msa0636540:intron 25.0%
!!! TAGTTTGATTTATTTTCCCT+AGG - chr4_3:27195553-27195572 Msa0636540:CDS 25.0%
! AAACGACTATTATTTAGAGA+CGG + chr4_3:27195053-27195072 None:intergenic 25.0%
! CTCTAAATAATAGTCGTTTA+AGG - chr4_3:27195054-27195073 Msa0636540:intron 25.0%
! CCACTAACTACAATTAATAA+GGG + chr4_3:27195153-27195172 None:intergenic 25.0%
! TCCACTAACTACAATTAATA+AGG + chr4_3:27195154-27195173 None:intergenic 25.0%
! AAAGAAACAATTATTGTGAG+CGG - chr4_3:27195284-27195303 Msa0636540:intron 25.0%
!!! TAGTTTGATTTATTTTCCCT+AGG - chr4_3:27195553-27195572 Msa0636540:CDS 25.0%
!!! AAAGAAGTGCTCTCTTATTT+TGG - chr4_3:27194712-27194731 Msa0636540:three_prime_UTR 30.0%
TTTCATCAAAGACATTAGCA+AGG - chr4_3:27194762-27194781 Msa0636540:three_prime_UTR 30.0%
! GTTGTTCTTGTATTGATGTA+TGG - chr4_3:27194895-27194914 Msa0636540:CDS 30.0%
AAGTAAAACCTGCAAAATTG+TGG + chr4_3:27195001-27195020 None:intergenic 30.0%
CCCTTATTAATTGTAGTTAG+TGG - chr4_3:27195150-27195169 Msa0636540:intron 30.0%
!!! AAAGAAGTGCTCTCTTATTT+TGG - chr4_3:27194712-27194731 Msa0636540:three_prime_UTR 30.0%
TTTCATCAAAGACATTAGCA+AGG - chr4_3:27194762-27194781 Msa0636540:three_prime_UTR 30.0%
! GTTGTTCTTGTATTGATGTA+TGG - chr4_3:27194895-27194914 Msa0636540:CDS 30.0%
AAGTAAAACCTGCAAAATTG+TGG + chr4_3:27195001-27195020 None:intergenic 30.0%
CCCTTATTAATTGTAGTTAG+TGG - chr4_3:27195150-27195169 Msa0636540:intron 30.0%
GCGCAAATGCAATAACAAAA+TGG + chr4_3:27194842-27194861 None:intergenic 35.0%
GACTATTATTTAGAGACGGA+GGG + chr4_3:27195049-27195068 None:intergenic 35.0%
AGTTAGTGGAGTAGTTAAGT+TGG - chr4_3:27195164-27195183 Msa0636540:intron 35.0%
AAACAATTATTGTGAGCGGA+GGG - chr4_3:27195288-27195307 Msa0636540:intron 35.0%
!! CGTTAGGTTGGATCAATTTA+CGG - chr4_3:27195474-27195493 Msa0636540:intron 35.0%
GATCAATTTACGGATGAAGT+TGG - chr4_3:27195484-27195503 Msa0636540:intron 35.0%
GCGCAAATGCAATAACAAAA+TGG + chr4_3:27194842-27194861 None:intergenic 35.0%
GACTATTATTTAGAGACGGA+GGG + chr4_3:27195049-27195068 None:intergenic 35.0%
AGTTAGTGGAGTAGTTAAGT+TGG - chr4_3:27195164-27195183 Msa0636540:intron 35.0%
AAACAATTATTGTGAGCGGA+GGG - chr4_3:27195288-27195307 Msa0636540:intron 35.0%
!! CGTTAGGTTGGATCAATTTA+CGG - chr4_3:27195474-27195493 Msa0636540:intron 35.0%
GATCAATTTACGGATGAAGT+TGG - chr4_3:27195484-27195503 Msa0636540:intron 35.0%
AATAAGAAGAGACTCCTTGC+TGG - chr4_3:27194951-27194970 Msa0636540:CDS 40.0%
!! GACGGAGGGAGTATTATTTA+TGG + chr4_3:27195035-27195054 None:intergenic 40.0%
CGACTATTATTTAGAGACGG+AGG + chr4_3:27195050-27195069 None:intergenic 40.0%
GAAACAATTATTGTGAGCGG+AGG - chr4_3:27195287-27195306 Msa0636540:intron 40.0%
AGACTCCTTGCAGAAAATTG+TGG - chr4_3:27195386-27195405 Msa0636540:CDS 40.0%
GACTCCTTGCAGAAAATTGT+GGG - chr4_3:27195387-27195406 Msa0636540:CDS 40.0%
! TACTCCCACAATTTTCTGCA+AGG + chr4_3:27195394-27195413 None:intergenic 40.0%
TTGCAGAAAATTGTGGGAGT+AGG - chr4_3:27195393-27195412 Msa0636540:intron 40.0%
AATTGTGGGAGTAGGATACA+TGG - chr4_3:27195401-27195420 Msa0636540:intron 40.0%
CATAGAAAATTGTGGCAACG+TGG + chr4_3:27195443-27195462 None:intergenic 40.0%
CTATGAATGATATGGGCGTT+AGG - chr4_3:27195458-27195477 Msa0636540:intron 40.0%
AATAAGAAGAGACTCCTTGC+TGG - chr4_3:27194951-27194970 Msa0636540:CDS 40.0%
!! GACGGAGGGAGTATTATTTA+TGG + chr4_3:27195035-27195054 None:intergenic 40.0%
CGACTATTATTTAGAGACGG+AGG + chr4_3:27195050-27195069 None:intergenic 40.0%
GAAACAATTATTGTGAGCGG+AGG - chr4_3:27195287-27195306 Msa0636540:intron 40.0%
AGACTCCTTGCAGAAAATTG+TGG - chr4_3:27195386-27195405 Msa0636540:CDS 40.0%
GACTCCTTGCAGAAAATTGT+GGG - chr4_3:27195387-27195406 Msa0636540:CDS 40.0%
! TACTCCCACAATTTTCTGCA+AGG + chr4_3:27195394-27195413 None:intergenic 40.0%
TTGCAGAAAATTGTGGGAGT+AGG - chr4_3:27195393-27195412 Msa0636540:intron 40.0%
AATTGTGGGAGTAGGATACA+TGG - chr4_3:27195401-27195420 Msa0636540:intron 40.0%
CATAGAAAATTGTGGCAACG+TGG + chr4_3:27195443-27195462 None:intergenic 40.0%
CTATGAATGATATGGGCGTT+AGG - chr4_3:27195458-27195477 Msa0636540:intron 40.0%
TATGGGGAAGTTCACGTACT+TGG + chr4_3:27194815-27194834 None:intergenic 45.0%
TACATGGTCCTCATGCTGAT+CGG - chr4_3:27195417-27195436 Msa0636540:intron 45.0%
GAATGATATGGGCGTTAGGT+TGG - chr4_3:27195462-27195481 Msa0636540:intron 45.0%
TATGGGGAAGTTCACGTACT+TGG + chr4_3:27194815-27194834 None:intergenic 45.0%
TACATGGTCCTCATGCTGAT+CGG - chr4_3:27195417-27195436 Msa0636540:intron 45.0%
GAATGATATGGGCGTTAGGT+TGG - chr4_3:27195462-27195481 Msa0636540:intron 45.0%
AGAAGAGACTCCTTGCTGGT+AGG - chr4_3:27194955-27194974 Msa0636540:CDS 50.0%
TATCAGGCTTCCTACCAGCA+AGG + chr4_3:27194968-27194987 None:intergenic 50.0%
AGGAAGCCTGATAAAGCACG+TGG - chr4_3:27194975-27194994 Msa0636540:intron 50.0%
TTGTGGCCACGTGCTTTATC+AGG + chr4_3:27194984-27195003 None:intergenic 50.0%
ACACTCGAGCTTTCGACCTA+GGG + chr4_3:27195572-27195591 None:intergenic 50.0%
AACACTCGAGCTTTCGACCT+AGG + chr4_3:27195573-27195592 None:intergenic 50.0%
! TCGAAAGCTCGAGTGTTGCA+CGG - chr4_3:27195576-27195595 Msa0636540:CDS 50.0%
AGAAGAGACTCCTTGCTGGT+AGG - chr4_3:27194955-27194974 Msa0636540:CDS 50.0%
TATCAGGCTTCCTACCAGCA+AGG + chr4_3:27194968-27194987 None:intergenic 50.0%
AGGAAGCCTGATAAAGCACG+TGG - chr4_3:27194975-27194994 Msa0636540:intron 50.0%
TTGTGGCCACGTGCTTTATC+AGG + chr4_3:27194984-27195003 None:intergenic 50.0%
ACACTCGAGCTTTCGACCTA+GGG + chr4_3:27195572-27195591 None:intergenic 50.0%
AACACTCGAGCTTTCGACCT+AGG + chr4_3:27195573-27195592 None:intergenic 50.0%
! TCGAAAGCTCGAGTGTTGCA+CGG - chr4_3:27195576-27195595 Msa0636540:CDS 50.0%
! GCACGTGGCCACAATTTTGC+AGG - chr4_3:27194990-27195009 Msa0636540:intron 55.0%
! CGAAAGCTCGAGTGTTGCAC+GGG - chr4_3:27195577-27195596 Msa0636540:CDS 55.0%
! GCACGTGGCCACAATTTTGC+AGG - chr4_3:27194990-27195009 Msa0636540:intron 55.0%
! CGAAAGCTCGAGTGTTGCAC+GGG - chr4_3:27195577-27195596 Msa0636540:CDS 55.0%
CAACGTGGCCGATCAGCATG+AGG + chr4_3:27195428-27195447 None:intergenic 60.0%
CAACGTGGCCGATCAGCATG+AGG + chr4_3:27195428-27195447 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 27194694 27195646 27194694 ID=Msa0636540;Name=Msa0636540
chr4_3 mRNA 27194694 27195646 27194694 ID=Msa0636540-mRNA-1;Parent=Msa0636540;Name=Msa0636540-mRNA-1;_AED=0.00;_eAED=0.00;_QI=10|1|1|1|1|1|3|183|97
chr4_3 exon 27194694 27194977 27194694 ID=Msa0636540-mRNA-1:exon:6754;Parent=Msa0636540-mRNA-1
chr4_3 exon 27195329 27195409 27195329 ID=Msa0636540-mRNA-1:exon:6753;Parent=Msa0636540-mRNA-1
chr4_3 exon 27195525 27195646 27195525 ID=Msa0636540-mRNA-1:exon:6752;Parent=Msa0636540-mRNA-1
chr4_3 five_prime_UTR 27195637 27195646 27195637 ID=Msa0636540-mRNA-1:five_prime_utr;Parent=Msa0636540-mRNA-1
chr4_3 CDS 27195525 27195636 27195525 ID=Msa0636540-mRNA-1:cds;Parent=Msa0636540-mRNA-1
chr4_3 CDS 27195329 27195409 27195329 ID=Msa0636540-mRNA-1:cds;Parent=Msa0636540-mRNA-1
chr4_3 CDS 27194877 27194977 27194877 ID=Msa0636540-mRNA-1:cds;Parent=Msa0636540-mRNA-1
chr4_3 three_prime_UTR 27194694 27194876 27194694 ID=Msa0636540-mRNA-1:three_prime_utr;Parent=Msa0636540-mRNA-1
Gene Sequence

>Msa0636540

ATGGCTTCAAAGAAGTGCTCTCTTATTTTGGTAGTTCTCTTTCTTTGTCTGTTGAGTTTTTCATCAAAGACATTAGCAAGGAACATTGCGAATGCTTCGAAACTTTATCCAATTTCAGATAAAATAGGTCTGAATAAGAAGAGACTCCTTGCTGGTAGGAAGCCTGATAAAGCACGTGGCCACAATTTTGCAGAAGAAATAAATCTGAATAAGAAGAGACTCCTTGCAGAAAATTGTGGGAGTAGGATACATGGTCCTCATGCTGATCGGCCACGTTGCCACAATTTTCTATGA

Protein sequence

>Msa0636540

MASKKCSLILVVLFLCLLSFSSKTLARNIANASKLYPISDKIGLNKKRLLAGRKPDKARGHNFAEEINLNKKRLLAENCGSRIHGPHADRPRCHNFL*