Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0831420 | XP_003626798.1 | 44.578 | 166 | 61 | 6 | 135 | 278 | 23 | 179 | 1.01e-25 | 114 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0831420 | sp|Q9LY41|ATL4_ARATH | 42.017 | 119 | 56 | 4 | 170 | 281 | 88 | 200 | 1.53e-17 | 84.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0831420 | G7LDE0 | 44.578 | 166 | 61 | 6 | 135 | 278 | 23 | 179 | 4.81e-26 | 114 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0008340 | Msa0831420 | 0.800651 | 1.303905e-48 | -8.615850e-47 |
| Msa0011720 | Msa0831420 | 0.809309 | 2.016902e-50 | -8.615850e-47 |
| Msa0011850 | Msa0831420 | 0.800321 | 1.522548e-48 | -8.615850e-47 |
| Msa0050730 | Msa0831420 | 0.820024 | 8.532132e-53 | -8.615850e-47 |
| Msa0050760 | Msa0831420 | 0.822515 | 2.273093e-53 | -8.615850e-47 |
| Msa0094100 | Msa0831420 | 0.803996 | 2.669746e-49 | -8.615850e-47 |
| Msa0096450 | Msa0831420 | 0.825519 | 4.485178e-54 | -8.615850e-47 |
| Msa0105340 | Msa0831420 | 0.804789 | 1.824577e-49 | -8.615850e-47 |
| Msa0105420 | Msa0831420 | 0.802815 | 4.690671e-49 | -8.615850e-47 |
| Msa0610590 | Msa0831420 | 0.833397 | 5.460339e-56 | -8.615850e-47 |
| Msa0651190 | Msa0831420 | 0.824331 | 8.552740e-54 | -8.615850e-47 |
| Msa0743260 | Msa0831420 | 0.818598 | 1.803114e-52 | -8.615850e-47 |
| Msa0749490 | Msa0831420 | 0.988605 | 1.118737e-174 | -8.615850e-47 |
| Msa0749520 | Msa0831420 | 0.902799 | 6.699323e-79 | -8.615850e-47 |
| Msa0767080 | Msa0831420 | 0.828689 | 7.820647e-55 | -8.615850e-47 |
| Msa0775260 | Msa0831420 | 0.808848 | 2.531475e-50 | -8.615850e-47 |
| Msa0793920 | Msa0831420 | 0.962049 | 2.043113e-120 | -8.615850e-47 |
| Msa0831420 | Msa0846810 | 0.802571 | 5.265654e-49 | -8.615850e-47 |
| Msa0831420 | Msa0853170 | 0.812137 | 4.932956e-51 | -8.615850e-47 |
| Msa0831420 | Msa0882510 | 0.808965 | 2.389657e-50 | -8.615850e-47 |
| Msa0831420 | Msa0896210 | 0.828712 | 7.719518e-55 | -8.615850e-47 |
| Msa0831420 | Msa0896220 | 0.825007 | 5.927280e-54 | -8.615850e-47 |
| Msa0831420 | Msa0927670 | 0.807925 | 3.983164e-50 | -8.615850e-47 |
| Msa0831420 | Msa0960160 | 0.800464 | 1.423422e-48 | -8.615850e-47 |
| Msa0831420 | Msa0991670 | 0.812161 | 4.872213e-51 | -8.615850e-47 |
| Msa0831420 | Msa0996080 | 0.809506 | 1.829782e-50 | -8.615850e-47 |
| Msa0831420 | Msa1026760 | 0.808029 | 3.785720e-50 | -8.615850e-47 |
| Msa0831420 | Msa1026790 | 0.808470 | 3.048538e-50 | -8.615850e-47 |
| Msa0831420 | Msa1030970 | 0.800438 | 1.441010e-48 | -8.615850e-47 |
| Msa0831420 | Msa1075220 | -0.800075 | 1.708536e-48 | -8.615850e-47 |
| Msa0831420 | Msa1085300 | 0.814168 | 1.767575e-51 | -8.615850e-47 |
| Msa0831420 | Msa1097840 | 0.812297 | 4.550290e-51 | -8.615850e-47 |
| Msa0831420 | Msa1131940 | -0.805636 | 1.213408e-49 | -8.615850e-47 |
| Msa0831420 | Msa1137600 | 0.800316 | 1.526223e-48 | -8.615850e-47 |
| Msa0831420 | Msa1175330 | 0.804281 | 2.328633e-49 | -8.615850e-47 |
| Msa0831420 | Msa1211150 | -0.804414 | 2.185403e-49 | -8.615850e-47 |
| Msa0831420 | Msa1217810 | 0.808145 | 3.575399e-50 | -8.615850e-47 |
| Msa0831420 | Msa1233890 | 0.808590 | 2.873811e-50 | -8.615850e-47 |
| Msa0831420 | Msa1235630 | 0.804273 | 2.338009e-49 | -8.615850e-47 |
| Msa0831420 | Msa1252070 | 0.803541 | 3.318765e-49 | -8.615850e-47 |
| Msa0831420 | Msa1277280 | 0.800865 | 1.179087e-48 | -8.615850e-47 |
| Msa0831420 | Msa1280130 | 0.804442 | 2.155627e-49 | -8.615850e-47 |
| Msa0831420 | Msa1299300 | -0.800209 | 1.604156e-48 | -8.615850e-47 |
| Msa0831420 | Msa1300350 | -0.811968 | 5.367708e-51 | -8.615850e-47 |
| Msa0831420 | Msa1340650 | 0.818089 | 2.351110e-52 | -8.615850e-47 |
| Msa0831420 | Msa1347620 | 0.802373 | 5.786167e-49 | -8.615850e-47 |
| Msa0831420 | Msa1408790 | 0.810383 | 1.184369e-50 | -8.615850e-47 |
| Msa0831420 | Msa1436040 | 0.804392 | 2.208161e-49 | -8.615850e-47 |
| Msa0831420 | Msa1440600 | 0.823860 | 1.103323e-53 | -8.615850e-47 |
| Msa0831420 | Msa1441190 | 0.800756 | 1.241202e-48 | -8.615850e-47 |
| Msa0230770 | Msa0831420 | 0.801737 | 7.819771e-49 | -8.615850e-47 |
| Msa0449760 | Msa0831420 | 0.830025 | 3.704260e-55 | -8.615850e-47 |
| Msa0449830 | Msa0831420 | 0.811517 | 6.728214e-51 | -8.615850e-47 |
| Msa0480320 | Msa0831420 | 0.812524 | 4.059959e-51 | -8.615850e-47 |
| Msa0243690 | Msa0831420 | 0.802002 | 6.898456e-49 | -8.615850e-47 |
| Msa0282250 | Msa0831420 | 0.817835 | 2.683255e-52 | -8.615850e-47 |
| Msa0296760 | Msa0831420 | 0.835257 | 1.863820e-56 | -8.615850e-47 |
| Msa0315290 | Msa0831420 | 0.820729 | 5.879664e-53 | -8.615850e-47 |
| Msa0328210 | Msa0831420 | 0.800810 | 1.209969e-48 | -8.615850e-47 |
| Msa0335040 | Msa0831420 | 0.805404 | 1.357163e-49 | -8.615850e-47 |
| Msa0578970 | Msa0831420 | 0.828994 | 6.597113e-55 | -8.615850e-47 |
| Msa0587690 | Msa0831420 | 0.809752 | 1.619798e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0831420 | MtrunA17_Chr8g0336881 | 44.578 | 166 | 61 | 6 | 135 | 278 | 23 | 179 | 9.26e-30 | 114 |
| Msa0831420 | MtrunA17_Chr8g0347461 | 50.000 | 112 | 44 | 5 | 176 | 284 | 117 | 219 | 6.46e-21 | 90.1 |
| Msa0831420 | MtrunA17_Chr2g0301871 | 47.321 | 112 | 47 | 5 | 179 | 284 | 63 | 168 | 7.34e-21 | 90.1 |
| Msa0831420 | MtrunA17_Chr5g0409211 | 32.031 | 128 | 71 | 7 | 131 | 246 | 13 | 136 | 4.37e-14 | 68.9 |
| Msa0831420 | MtrunA17_Chr3g0133001 | 40.541 | 74 | 32 | 3 | 175 | 246 | 135 | 198 | 1.71e-12 | 67.0 |
| Msa0831420 | MtrunA17_Chr5g0409201 | 30.303 | 132 | 77 | 5 | 126 | 246 | 8 | 135 | 3.05e-12 | 63.5 |
| Msa0831420 | MtrunA17_Chr5g0427431 | 35.789 | 95 | 45 | 4 | 159 | 246 | 117 | 202 | 6.43e-12 | 65.5 |
| Msa0831420 | MtrunA17_Chr6g0486651 | 31.902 | 163 | 82 | 7 | 100 | 246 | 12 | 161 | 1.29e-11 | 64.3 |
| Msa0831420 | MtrunA17_Chr3g0123641 | 28.276 | 145 | 86 | 5 | 132 | 269 | 2 | 135 | 2.01e-11 | 62.8 |
| Msa0831420 | MtrunA17_Chr1g0148631 | 45.455 | 55 | 30 | 0 | 202 | 256 | 338 | 392 | 2.22e-11 | 63.9 |
| Msa0831420 | MtrunA17_Chr8g0388231 | 30.534 | 131 | 74 | 6 | 127 | 247 | 257 | 380 | 2.35e-11 | 63.9 |
| Msa0831420 | MtrunA17_Chr3g0123631 | 29.008 | 131 | 80 | 5 | 132 | 255 | 2 | 126 | 2.70e-11 | 61.6 |
| Msa0831420 | MtrunA17_Chr7g0275601 | 27.200 | 125 | 78 | 4 | 136 | 247 | 27 | 151 | 2.85e-11 | 60.8 |
| Msa0831420 | MtrunA17_Chr6g0451051 | 30.400 | 125 | 74 | 3 | 136 | 259 | 9 | 121 | 4.23e-11 | 60.1 |
| Msa0831420 | MtrunA17_Chr8g0364881 | 46.753 | 77 | 31 | 3 | 176 | 251 | 74 | 141 | 4.93e-11 | 62.4 |
| Msa0831420 | MtrunA17_Chr2g0305161 | 40.678 | 59 | 35 | 0 | 191 | 249 | 128 | 186 | 5.24e-11 | 60.8 |
| Msa0831420 | MtrunA17_Chr3g0112041 | 42.105 | 57 | 31 | 1 | 192 | 246 | 198 | 254 | 6.10e-11 | 61.6 |
| Msa0831420 | MtrunA17_Chr6g0451161 | 44.156 | 77 | 33 | 3 | 174 | 249 | 137 | 204 | 6.34e-11 | 62.4 |
| Msa0831420 | MtrunA17_Chr4g0031911 | 43.396 | 53 | 28 | 1 | 196 | 246 | 198 | 250 | 7.32e-11 | 61.6 |
| Msa0831420 | MtrunA17_Chr7g0274741 | 47.059 | 51 | 26 | 1 | 204 | 253 | 98 | 148 | 7.53e-11 | 60.1 |
| Msa0831420 | MtrunA17_Chr3g0138451 | 35.000 | 80 | 50 | 2 | 177 | 255 | 289 | 367 | 8.68e-11 | 62.0 |
| Msa0831420 | MtrunA17_Chr4g0059161 | 38.824 | 85 | 38 | 3 | 162 | 246 | 206 | 276 | 8.98e-11 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0831420 | AT3G60220.1 | 42.017 | 119 | 56 | 4 | 170 | 281 | 88 | 200 | 1.56e-18 | 84.7 |
| Msa0831420 | AT4G32600.1 | 34.545 | 110 | 51 | 3 | 165 | 256 | 308 | 414 | 2.41e-13 | 70.1 |
| Msa0831420 | AT4G32600.2 | 34.545 | 110 | 51 | 3 | 165 | 256 | 308 | 414 | 2.41e-13 | 70.1 |
| Msa0831420 | AT1G35330.1 | 31.395 | 172 | 86 | 8 | 131 | 279 | 44 | 206 | 5.07e-13 | 68.6 |
| Msa0831420 | AT3G48030.1 | 46.250 | 80 | 34 | 3 | 176 | 254 | 186 | 257 | 6.82e-13 | 68.6 |
| Msa0831420 | AT1G80400.1 | 33.333 | 102 | 53 | 2 | 168 | 254 | 303 | 404 | 6.88e-13 | 68.9 |
| Msa0831420 | AT3G18930.2 | 41.558 | 77 | 43 | 2 | 175 | 249 | 125 | 201 | 7.21e-13 | 68.6 |
| Msa0831420 | AT3G18930.3 | 41.558 | 77 | 43 | 2 | 175 | 249 | 125 | 201 | 7.21e-13 | 68.6 |
| Msa0831420 | AT3G18930.1 | 41.558 | 77 | 43 | 2 | 175 | 249 | 125 | 201 | 7.21e-13 | 68.6 |
| Msa0831420 | AT3G48030.2 | 46.250 | 80 | 34 | 3 | 176 | 254 | 108 | 179 | 9.65e-13 | 67.4 |
| Msa0831420 | AT4G28890.1 | 43.421 | 76 | 33 | 3 | 176 | 250 | 103 | 169 | 1.02e-12 | 68.2 |
| Msa0831420 | AT5G57750.1 | 47.945 | 73 | 29 | 3 | 176 | 247 | 101 | 165 | 1.70e-12 | 65.9 |
| Msa0831420 | AT4G11680.1 | 33.673 | 98 | 48 | 3 | 176 | 256 | 293 | 390 | 1.87e-11 | 64.3 |
| Msa0831420 | AT1G20823.1 | 43.284 | 67 | 37 | 1 | 184 | 249 | 90 | 156 | 2.04e-11 | 62.4 |
| Msa0831420 | AT1G76410.1 | 33.333 | 120 | 72 | 4 | 132 | 249 | 36 | 149 | 2.57e-11 | 62.0 |
| Msa0831420 | AT1G23980.1 | 44.156 | 77 | 33 | 3 | 174 | 249 | 122 | 189 | 3.23e-11 | 63.5 |
| Msa0831420 | AT2G20030.1 | 35.714 | 112 | 57 | 5 | 176 | 286 | 104 | 201 | 3.37e-11 | 63.5 |
| Msa0831420 | AT2G15580.1 | 53.488 | 43 | 20 | 0 | 204 | 246 | 149 | 191 | 4.58e-11 | 61.2 |
| Msa0831420 | AT4G35480.1 | 43.750 | 64 | 34 | 2 | 193 | 255 | 102 | 164 | 7.04e-11 | 60.8 |
| Msa0831420 | AT3G47990.3 | 36.000 | 100 | 50 | 3 | 164 | 262 | 150 | 236 | 7.12e-11 | 62.0 |
| Msa0831420 | AT3G47990.5 | 36.000 | 100 | 50 | 3 | 164 | 262 | 150 | 236 | 7.12e-11 | 62.0 |
| Msa0831420 | AT3G47990.4 | 36.000 | 100 | 50 | 3 | 164 | 262 | 150 | 236 | 7.12e-11 | 62.0 |
| Msa0831420 | AT4G40070.1 | 26.471 | 204 | 116 | 10 | 101 | 288 | 14 | 199 | 7.13e-11 | 62.4 |
| Msa0831420 | AT5G05810.1 | 40.541 | 74 | 32 | 3 | 175 | 246 | 125 | 188 | 7.97e-11 | 62.4 |
| Msa0831420 | AT3G47990.2 | 36.000 | 100 | 50 | 3 | 164 | 262 | 191 | 277 | 9.98e-11 | 62.0 |
Find 72 sgRNAs with CRISPR-Local
Find 101 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAGGCTCACGTTTCCTATT+TGG | 0.229228 | 5_4:+4418331 | None:intergenic |
| GTCGTCCGTGGTGGTGGCGT+TGG | 0.373084 | 5_4:+4418660 | None:intergenic |
| GCCAACGAGGTTTGCATCCA+TGG | 0.419497 | 5_4:+4418761 | None:intergenic |
| GACAGTATCACCGTCCAAAT+AGG | 0.426859 | 5_4:-4418345 | Msa0831420:CDS |
| TTTAACAGCTTGAGTATTGT+TGG | 0.429356 | 5_4:+4419517 | None:intergenic |
| CCGTGGTGGTGGCGTTGGGT+TGG | 0.435131 | 5_4:+4418665 | None:intergenic |
| TTGAGGAGAAACGGAAGAGA+GGG | 0.448642 | 5_4:+4418604 | None:intergenic |
| CGATTCCAACACCGACCTCT+TGG | 0.451236 | 5_4:-4418391 | Msa0831420:CDS |
| TCGTCCGTGGTGGTGGCGTT+GGG | 0.454118 | 5_4:+4418661 | None:intergenic |
| CAACCATTGTTGAGACATGA+TGG | 0.466296 | 5_4:+4419493 | None:intergenic |
| CAAGAGGTCGGTGTTGGAAT+CGG | 0.472953 | 5_4:+4418392 | None:intergenic |
| CTTCCGACGGCTCCGTCAAA+CGG | 0.483665 | 5_4:+4418281 | None:intergenic |
| ATTGTTGGCCACGGATGGAT+TGG | 0.484950 | 5_4:+4419532 | None:intergenic |
| GTAACAGTCGTCCGTGGTGG+TGG | 0.486562 | 5_4:+4418654 | None:intergenic |
| GGTGGGTCCGATGGTGGAAA+AGG | 0.488594 | 5_4:+4418569 | None:intergenic |
| GAGTGAAAAGCGTGGAAACA+TGG | 0.489948 | 5_4:+4418477 | None:intergenic |
| ATTGAGGAGAAACGGAAGAG+AGG | 0.499378 | 5_4:+4418603 | None:intergenic |
| AAATAGGAAACGTGAGCCTC+CGG | 0.500001 | 5_4:-4418329 | Msa0831420:CDS |
| GGTTGGAACGGCGGTGATGA+AGG | 0.500084 | 5_4:+4418682 | None:intergenic |
| TGAGTATTGTTGGCCACGGA+TGG | 0.500791 | 5_4:+4419527 | None:intergenic |
| CCGATCTCGAATCCGTTTGA+CGG | 0.501263 | 5_4:-4418293 | None:intergenic |
| CGCGAGAAATTGAGGAGAAA+CGG | 0.509382 | 5_4:+4418595 | None:intergenic |
| GACATCGCCGGTGGGTCCGA+TGG | 0.510113 | 5_4:+4418560 | None:intergenic |
| AAAGGTGCCGCGAGAAATTG+AGG | 0.516065 | 5_4:+4418587 | None:intergenic |
| AAACGGAGGTGAGCGGAGAA+TGG | 0.521841 | 5_4:+4418368 | None:intergenic |
| ACCACCACGGACGACTGTTA+CGG | 0.525467 | 5_4:-4418652 | Msa0831420:CDS |
| AACGGATTCGAGATCGGTGG+CGG | 0.526950 | 5_4:+4418299 | None:intergenic |
| TTGTATGCTAGAATAATCAT+TGG | 0.536757 | 5_4:+4419572 | None:intergenic |
| ATCGCCGGTGGGTCCGATGG+TGG | 0.537352 | 5_4:+4418563 | None:intergenic |
| AATGCTACAAACATATAAGA+AGG | 0.541568 | 5_4:-4418896 | Msa0831420:CDS |
| GTGGTGGCGTTGGGTTGGAA+CGG | 0.541959 | 5_4:+4418670 | None:intergenic |
| AGCTTGAGTATTGTTGGCCA+CGG | 0.547462 | 5_4:+4419523 | None:intergenic |
| GTGGCGTTGGGTTGGAACGG+CGG | 0.551299 | 5_4:+4418673 | None:intergenic |
| AATGGCCAAGAGGTCGGTGT+TGG | 0.552990 | 5_4:+4418386 | None:intergenic |
| GATCGGTGGCGGTTTCGCGC+CGG | 0.557217 | 5_4:+4418310 | None:intergenic |
| CGGTGGCGGTTTCGCGCCGG+AGG | 0.559519 | 5_4:+4418313 | None:intergenic |
| TCCATGGATGCAAACCTCGT+TGG | 0.561231 | 5_4:-4418762 | Msa0831420:CDS |
| ACTCTTACTGCATAAGCAGA+TGG | 0.563965 | 5_4:-4418458 | Msa0831420:CDS |
| GCAATTGTGAAACGAGTGAA+AGG | 0.566267 | 5_4:+4418712 | None:intergenic |
| CAATGAGTCCCTCGTGCGTG+TGG | 0.572775 | 5_4:+4418859 | None:intergenic |
| AAAGCAAAACCACACGCACG+AGG | 0.576130 | 5_4:-4418868 | Msa0831420:CDS |
| AACGAAGCATGTCGTTGTTG+CGG | 0.577997 | 5_4:+4418505 | None:intergenic |
| GTCATCGTCGACATCGCCGG+TGG | 0.578999 | 5_4:+4418551 | None:intergenic |
| CCGTCAAACGGATTCGAGAT+CGG | 0.580404 | 5_4:+4418293 | None:intergenic |
| ACAGTCATCGTCGACATCGC+CGG | 0.580449 | 5_4:+4418548 | None:intergenic |
| GACGACTGTTACGGAAACAT+TGG | 0.585613 | 5_4:-4418643 | Msa0831420:CDS |
| CTAGCATACAAGTTTGCTCG+TGG | 0.587303 | 5_4:-4419560 | Msa0831420:CDS |
| AGTGAAAAGCGTGGAAACAT+GGG | 0.589501 | 5_4:+4418478 | None:intergenic |
| CCATGGAGTGAAACGAAAAG+AGG | 0.589662 | 5_4:+4418778 | None:intergenic |
| TGGACGGTGATACTGTCAAA+CGG | 0.597045 | 5_4:+4418351 | None:intergenic |
| GCTCACGTTTCCTATTTGGA+CGG | 0.601842 | 5_4:+4418335 | None:intergenic |
| CGTTTCTCCTCAATTTCTCG+CGG | 0.602815 | 5_4:-4418594 | Msa0831420:CDS |
| AGAGAGAAAGAAATGCGTTG+TGG | 0.618174 | 5_4:+4418807 | None:intergenic |
| GCGGAGAATGGCCAAGAGGT+CGG | 0.629982 | 5_4:+4418380 | None:intergenic |
| AGAAACGGAAGAGAGGGAGT+CGG | 0.630762 | 5_4:+4418610 | None:intergenic |
| TCCGTAACAGTCGTCCGTGG+TGG | 0.641987 | 5_4:+4418651 | None:intergenic |
| GTGAAAGGAAGAGAAGCGCG+AGG | 0.644848 | 5_4:+4418727 | None:intergenic |
| TGCAGTAAGAGTGAAAAGCG+TGG | 0.645395 | 5_4:+4418469 | None:intergenic |
| GTTTCCGTAACAGTCGTCCG+TGG | 0.645896 | 5_4:+4418648 | None:intergenic |
| AAACCATCATGTCTCAACAA+TGG | 0.647470 | 5_4:-4419496 | Msa0831420:CDS |
| TACTGTCAAACGGAGGTGAG+CGG | 0.656369 | 5_4:+4418361 | None:intergenic |
| GAGAGGACATCGCGGCAGAG+AGG | 0.671800 | 5_4:+4418414 | None:intergenic |
| TCATCGTCGACATCGCCGGT+GGG | 0.672625 | 5_4:+4418552 | None:intergenic |
| TGGAGAAACCAATCCATCCG+TGG | 0.675735 | 5_4:-4419540 | Msa0831420:CDS |
| AAGCAAAACCACACGCACGA+GGG | 0.682505 | 5_4:-4418867 | Msa0831420:CDS |
| TCAAACGGATTCGAGATCGG+TGG | 0.696292 | 5_4:+4418296 | None:intergenic |
| CCAACCCAACGCCACCACCA+CGG | 0.700116 | 5_4:-4418665 | Msa0831420:CDS |
| GTGAGCGGAGAATGGCCAAG+AGG | 0.707678 | 5_4:+4418376 | None:intergenic |
| GGTCGGTGTTGGAATCGGAG+AGG | 0.717591 | 5_4:+4418397 | None:intergenic |
| GGAAAATGAGAAAGCCAACG+AGG | 0.717650 | 5_4:+4418748 | None:intergenic |
| TGGAATCGGAGAGGACATCG+CGG | 0.769053 | 5_4:+4418406 | None:intergenic |
| ACGGTGATACTGTCAAACGG+AGG | 0.799114 | 5_4:+4418354 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTATTAAACCATGTAATTTT+TGG | + | chr5_4:4418424-4418443 | None:intergenic | 15.0% |
| !!! | TCATTTTCTCAAATTATTAT+GGG | + | chr5_4:4418602-4418621 | None:intergenic | 15.0% |
| !!! | GTCATTTTCTCAAATTATTA+TGG | + | chr5_4:4418603-4418622 | None:intergenic | 20.0% |
| !!! | CTTTTGATTCTAATTGTTTT+TGG | + | chr5_4:4418727-4418746 | None:intergenic | 20.0% |
| !!! | CTATTTTTTCATGAAATTTC+AGG | + | chr5_4:4418830-4418849 | None:intergenic | 20.0% |
| !!! | ACCATGTAATTTTTGGTTTA+GGG | + | chr5_4:4418417-4418436 | None:intergenic | 25.0% |
| !!! | AACCATGTAATTTTTGGTTT+AGG | + | chr5_4:4418418-4418437 | None:intergenic | 25.0% |
| ! | ATGAAATTTCAGATGTATAG+TGG | + | chr5_4:4418473-4418492 | None:intergenic | 25.0% |
| ! | ATGAAACACCAATATAGATA+CGG | - | chr5_4:4418540-4418559 | Msa0831420:CDS | 25.0% |
| ! | GCATGTTTGTTTAGAAATTT+CGG | - | chr5_4:4418686-4418705 | Msa0831420:CDS | 25.0% |
| ! | TGGAAATTTATTCCATTGTA+AGG | + | chr5_4:4418796-4418815 | None:intergenic | 25.0% |
| ! | AATGCTACAAACATATAAGA+AGG | - | chr5_4:4418969-4418988 | Msa0831420:CDS | 25.0% |
| ! | TTTAACAGCTTGAGTATTGT+TGG | + | chr5_4:4418351-4418370 | None:intergenic | 30.0% |
| !!! | CCATGTAATTTTTGGTTTAG+GGG | + | chr5_4:4418416-4418435 | None:intergenic | 30.0% |
| GGTTTAATAAAGAACTCGTA+TGG | - | chr5_4:4418434-4418453 | Msa0831420:CDS | 30.0% | |
| AATTTCAGATGTATAGTGGT+TGG | + | chr5_4:4418469-4418488 | None:intergenic | 30.0% | |
| CATGAAAAATAACCAGACAA+TGG | - | chr5_4:4418490-4418509 | Msa0831420:CDS | 30.0% | |
| TGATATGTCCGTATCTATAT+TGG | + | chr5_4:4418551-4418570 | None:intergenic | 30.0% | |
| !!! | CATTGTAAGGTTTGTTTTGA+GGG | + | chr5_4:4418783-4418802 | None:intergenic | 30.0% |
| ! | TCGTATTTGTTCGTGTTTTA+TGG | + | chr5_4:4418883-4418902 | None:intergenic | 30.0% |
| AAACCATCATGTCTCAACAA+TGG | - | chr5_4:4418369-4418388 | Msa0831420:CDS | 35.0% | |
| CCCCTAAACCAAAAATTACA+TGG | - | chr5_4:4418413-4418432 | Msa0831420:CDS | 35.0% | |
| ! | TTTTGTTACTGTCCATTGTC+TGG | + | chr5_4:4418505-4418524 | None:intergenic | 35.0% |
| !!! | CCATTGTAAGGTTTGTTTTG+AGG | + | chr5_4:4418784-4418803 | None:intergenic | 35.0% |
| CCTCAAAACAAACCTTACAA+TGG | - | chr5_4:4418781-4418800 | Msa0831420:CDS | 35.0% | |
| ! | AATTTCAGGTGTATAGTGAC+TGG | + | chr5_4:4418816-4418835 | None:intergenic | 35.0% |
| ! | TGTTTTATGGTGTGATTGGT+TGG | + | chr5_4:4418870-4418889 | None:intergenic | 35.0% |
| ! | TTCGTGTTTTATGGTGTGAT+TGG | + | chr5_4:4418874-4418893 | None:intergenic | 35.0% |
| ! | GACACAACACTAACTTTTTG+CGG | - | chr5_4:4418909-4418928 | Msa0831420:CDS | 35.0% |
| ! | ACACAACACTAACTTTTTGC+GGG | - | chr5_4:4418910-4418929 | Msa0831420:CDS | 35.0% |
| CAACCATTGTTGAGACATGA+TGG | + | chr5_4:4418375-4418394 | None:intergenic | 40.0% | |
| TGAATGTGACTTCATGTGTC+AGG | - | chr5_4:4418628-4418647 | Msa0831420:CDS | 40.0% | |
| GAATGTGACTTCATGTGTCA+GGG | - | chr5_4:4418629-4418648 | Msa0831420:CDS | 40.0% | |
| AGAGAGAAAGAAATGCGTTG+TGG | + | chr5_4:4419061-4419080 | None:intergenic | 40.0% | |
| GCAATTGTGAAACGAGTGAA+AGG | + | chr5_4:4419156-4419175 | None:intergenic | 40.0% | |
| ! | AGTGAAAAGCGTGGAAACAT+GGG | + | chr5_4:4419390-4419409 | None:intergenic | 40.0% |
| ACTCTTACTGCATAAGCAGA+TGG | - | chr5_4:4419407-4419426 | Msa0831420:intron | 40.0% | |
| ! | CTAGCATACAAGTTTGCTCG+TGG | - | chr5_4:4418305-4418324 | Msa0831420:CDS | 45.0% |
| ! | AGCTTGAGTATTGTTGGCCA+CGG | + | chr5_4:4418345-4418364 | None:intergenic | 45.0% |
| GATAGCAGCAGCTATTGTTG+AGG | + | chr5_4:4418750-4418769 | None:intergenic | 45.0% | |
| CCATGGAGTGAAACGAAAAG+AGG | + | chr5_4:4419090-4419109 | None:intergenic | 45.0% | |
| ! | CCTCTTTTCGTTTCACTCCA+TGG | - | chr5_4:4419087-4419106 | Msa0831420:intron | 45.0% |
| GGAAAATGAGAAAGCCAACG+AGG | + | chr5_4:4419120-4419139 | None:intergenic | 45.0% | |
| GACGACTGTTACGGAAACAT+TGG | - | chr5_4:4419222-4419241 | Msa0831420:intron | 45.0% | |
| TTGAGGAGAAACGGAAGAGA+GGG | + | chr5_4:4419264-4419283 | None:intergenic | 45.0% | |
| ATTGAGGAGAAACGGAAGAG+AGG | + | chr5_4:4419265-4419284 | None:intergenic | 45.0% | |
| CGCGAGAAATTGAGGAGAAA+CGG | + | chr5_4:4419273-4419292 | None:intergenic | 45.0% | |
| CGTTTCTCCTCAATTTCTCG+CGG | - | chr5_4:4419271-4419290 | Msa0831420:intron | 45.0% | |
| AACGAAGCATGTCGTTGTTG+CGG | + | chr5_4:4419363-4419382 | None:intergenic | 45.0% | |
| ! | GAGTGAAAAGCGTGGAAACA+TGG | + | chr5_4:4419391-4419410 | None:intergenic | 45.0% |
| TGCAGTAAGAGTGAAAAGCG+TGG | + | chr5_4:4419399-4419418 | None:intergenic | 45.0% | |
| TGGACGGTGATACTGTCAAA+CGG | + | chr5_4:4419517-4419536 | None:intergenic | 45.0% | |
| GACAGTATCACCGTCCAAAT+AGG | - | chr5_4:4419520-4419539 | Msa0831420:CDS | 45.0% | |
| GCTCACGTTTCCTATTTGGA+CGG | + | chr5_4:4419533-4419552 | None:intergenic | 45.0% | |
| AAATAGGAAACGTGAGCCTC+CGG | - | chr5_4:4419536-4419555 | Msa0831420:CDS | 45.0% | |
| TGGAGAAACCAATCCATCCG+TGG | - | chr5_4:4418325-4418344 | Msa0831420:CDS | 50.0% | |
| ATTGTTGGCCACGGATGGAT+TGG | + | chr5_4:4418336-4418355 | None:intergenic | 50.0% | |
| ! | TGAGTATTGTTGGCCACGGA+TGG | + | chr5_4:4418341-4418360 | None:intergenic | 50.0% |
| AAAGCAAAACCACACGCACG+AGG | - | chr5_4:4418997-4419016 | Msa0831420:CDS | 50.0% | |
| AAGCAAAACCACACGCACGA+GGG | - | chr5_4:4418998-4419017 | Msa0831420:CDS | 50.0% | |
| TCCATGGATGCAAACCTCGT+TGG | - | chr5_4:4419103-4419122 | Msa0831420:intron | 50.0% | |
| AGAAACGGAAGAGAGGGAGT+CGG | + | chr5_4:4419258-4419277 | None:intergenic | 50.0% | |
| !! | AAAGGTGCCGCGAGAAATTG+AGG | + | chr5_4:4419281-4419300 | None:intergenic | 50.0% |
| !! | CAAGAGGTCGGTGTTGGAAT+CGG | + | chr5_4:4419476-4419495 | None:intergenic | 50.0% |
| TACTGTCAAACGGAGGTGAG+CGG | + | chr5_4:4419507-4419526 | None:intergenic | 50.0% | |
| ACGGTGATACTGTCAAACGG+AGG | + | chr5_4:4419514-4419533 | None:intergenic | 50.0% | |
| GGAGGCTCACGTTTCCTATT+TGG | + | chr5_4:4419537-4419556 | None:intergenic | 50.0% | |
| ! | TCAAACGGATTCGAGATCGG+TGG | + | chr5_4:4419572-4419591 | None:intergenic | 50.0% |
| GCCAACGAGGTTTGCATCCA+TGG | + | chr5_4:4419107-4419126 | None:intergenic | 55.0% | |
| ! | GTGAAAGGAAGAGAAGCGCG+AGG | + | chr5_4:4419141-4419160 | None:intergenic | 55.0% |
| ACCACCACGGACGACTGTTA+CGG | - | chr5_4:4419213-4419232 | Msa0831420:intron | 55.0% | |
| GTTTCCGTAACAGTCGTCCG+TGG | + | chr5_4:4419220-4419239 | None:intergenic | 55.0% | |
| ! | TTTTCCACCATCGGACCCAC+CGG | - | chr5_4:4419298-4419317 | Msa0831420:intron | 55.0% |
| ACAGTCATCGTCGACATCGC+CGG | + | chr5_4:4419320-4419339 | None:intergenic | 55.0% | |
| TGGAATCGGAGAGGACATCG+CGG | + | chr5_4:4419462-4419481 | None:intergenic | 55.0% | |
| CGATTCCAACACCGACCTCT+TGG | - | chr5_4:4419474-4419493 | Msa0831420:CDS | 55.0% | |
| AATGGCCAAGAGGTCGGTGT+TGG | + | chr5_4:4419482-4419501 | None:intergenic | 55.0% | |
| AAACGGAGGTGAGCGGAGAA+TGG | + | chr5_4:4419500-4419519 | None:intergenic | 55.0% | |
| ! | AACGGATTCGAGATCGGTGG+CGG | + | chr5_4:4419569-4419588 | None:intergenic | 55.0% |
| CAATGAGTCCCTCGTGCGTG+TGG | + | chr5_4:4419009-4419028 | None:intergenic | 60.0% | |
| GGTTGGAACGGCGGTGATGA+AGG | + | chr5_4:4419186-4419205 | None:intergenic | 60.0% | |
| !! | GTGGTGGCGTTGGGTTGGAA+CGG | + | chr5_4:4419198-4419217 | None:intergenic | 60.0% |
| GTAACAGTCGTCCGTGGTGG+TGG | + | chr5_4:4419214-4419233 | None:intergenic | 60.0% | |
| TCCGTAACAGTCGTCCGTGG+TGG | + | chr5_4:4419217-4419236 | None:intergenic | 60.0% | |
| ! | CGCGGCACCTTTTCCACCAT+CGG | - | chr5_4:4419289-4419308 | Msa0831420:intron | 60.0% |
| !! | GGTGGGTCCGATGGTGGAAA+AGG | + | chr5_4:4419299-4419318 | None:intergenic | 60.0% |
| TCATCGTCGACATCGCCGGT+GGG | + | chr5_4:4419316-4419335 | None:intergenic | 60.0% | |
| !! | GGTCGGTGTTGGAATCGGAG+AGG | + | chr5_4:4419471-4419490 | None:intergenic | 60.0% |
| GCGGAGAATGGCCAAGAGGT+CGG | + | chr5_4:4419488-4419507 | None:intergenic | 60.0% | |
| GTGAGCGGAGAATGGCCAAG+AGG | + | chr5_4:4419492-4419511 | None:intergenic | 60.0% | |
| !! | GTGGCGTTGGGTTGGAACGG+CGG | + | chr5_4:4419195-4419214 | None:intergenic | 65.0% |
| CCAACCCAACGCCACCACCA+CGG | - | chr5_4:4419200-4419219 | Msa0831420:intron | 65.0% | |
| !! | TCGTCCGTGGTGGTGGCGTT+GGG | + | chr5_4:4419207-4419226 | None:intergenic | 65.0% |
| GTCATCGTCGACATCGCCGG+TGG | + | chr5_4:4419317-4419336 | None:intergenic | 65.0% | |
| GAGAGGACATCGCGGCAGAG+AGG | + | chr5_4:4419454-4419473 | None:intergenic | 65.0% | |
| !! | CCGTGGTGGTGGCGTTGGGT+TGG | + | chr5_4:4419203-4419222 | None:intergenic | 70.0% |
| !! | GTCGTCCGTGGTGGTGGCGT+TGG | + | chr5_4:4419208-4419227 | None:intergenic | 70.0% |
| ATCGCCGGTGGGTCCGATGG+TGG | + | chr5_4:4419305-4419324 | None:intergenic | 70.0% | |
| GACATCGCCGGTGGGTCCGA+TGG | + | chr5_4:4419308-4419327 | None:intergenic | 70.0% | |
| ! | GATCGGTGGCGGTTTCGCGC+CGG | + | chr5_4:4419558-4419577 | None:intergenic | 70.0% |
| !! | CGGTGGCGGTTTCGCGCCGG+AGG | + | chr5_4:4419555-4419574 | None:intergenic | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_4 | gene | 4418296 | 4419591 | 4418296 | ID=Msa0831420;Name=Msa0831420 |
| chr5_4 | mRNA | 4418296 | 4419591 | 4418296 | ID=Msa0831420-mRNA-1;Parent=Msa0831420;Name=Msa0831420-mRNA-1;_AED=0.74;_eAED=0.74;_QI=0|0|0|1|0|0|2|0|288 |
| chr5_4 | exon | 4419453 | 4419591 | 4419453 | ID=Msa0831420-mRNA-1:exon:1852;Parent=Msa0831420-mRNA-1 |
| chr5_4 | exon | 4418296 | 4419023 | 4418296 | ID=Msa0831420-mRNA-1:exon:1851;Parent=Msa0831420-mRNA-1 |
| chr5_4 | CDS | 4419453 | 4419591 | 4419453 | ID=Msa0831420-mRNA-1:cds;Parent=Msa0831420-mRNA-1 |
| chr5_4 | CDS | 4418296 | 4419023 | 4418296 | ID=Msa0831420-mRNA-1:cds;Parent=Msa0831420-mRNA-1 |
| Gene Sequence |
| Protein sequence |
