AlfalfaGEDB | Msa0749520

M. sativa cultivar zhongmu-4 / Msa0749520

KEGG: K10663
GO: GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0051603 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575
PFAM: PF13639.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0749520	sp\|Q9LY41\|ATL4_ARATH	40.336	119	58	3	125	236	88	200	2.79e-17	82.8

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0749520	G7LDE0	45.399	163	64	6	90	233	23	179	6.41e-26	112

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0011720	Msa0749520	0.816660	4.930703e-52	-8.615850e-47
Msa0050760	Msa0749520	0.815193	1.047999e-51	-8.615850e-47
Msa0094100	Msa0749520	0.811543	6.643178e-51	-8.615850e-47
Msa0096450	Msa0749520	0.814978	1.169900e-51	-8.615850e-47
Msa0119850	Msa0749520	0.805822	1.108592e-49	-8.615850e-47
Msa0208630	Msa0749520	0.811839	5.727769e-51	-8.615850e-47
Msa0230770	Msa0749520	0.803125	4.046197e-49	-8.615850e-47
Msa0243710	Msa0749520	0.806508	7.952278e-50	-8.615850e-47
Msa0258760	Msa0749520	0.828539	8.497617e-55	-8.615850e-47
Msa0265820	Msa0749520	0.802725	4.895837e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0749520	MtrunA17_Chr8g0336881	45.399	163	64	6	90	233	23	179	1.23e-29	112
Msa0749520	MtrunA17_Chr2g0301871	47.321	112	47	5	134	239	63	168	6.95e-21	89.0
Msa0749520	MtrunA17_Chr8g0347461	49.107	112	45	5	131	239	117	219	3.37e-20	87.0
Msa0749520	MtrunA17_Chr5g0409211	32.031	128	71	7	86	201	13	136	1.66e-14	69.3
Msa0749520	MtrunA17_Chr5g0409201	30.303	132	77	5	81	201	8	135	1.35e-12	63.9
Msa0749520	MtrunA17_Chr3g0133001	40.541	74	32	3	130	201	135	198	1.85e-12	66.2
Msa0749520	MtrunA17_Chr5g0427431	35.789	95	45	4	114	201	117	202	2.50e-12	65.9
Msa0749520	MtrunA17_Chr3g0123641	28.276	145	86	5	87	224	2	135	5.42e-12	63.5
Msa0749520	MtrunA17_Chr3g0123631	28.276	145	86	5	87	224	2	135	6.11e-12	62.8
Msa0749520	MtrunA17_Chr6g0451051	32.000	125	72	4	91	214	9	121	6.72e-12	62.0

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0749520	AT3G60220.1	40.336	119	58	3	125	236	88	200	2.84e-18	82.8
Msa0749520	AT4G32600.1	41.429	70	40	1	142	211	346	414	6.56e-14	70.9
Msa0749520	AT4G32600.2	41.429	70	40	1	142	211	346	414	6.56e-14	70.9
Msa0749520	AT1G80400.1	33.333	102	53	2	123	209	303	404	2.03e-13	69.3
Msa0749520	AT3G18930.2	41.558	77	43	2	130	204	125	201	3.75e-13	68.6
Msa0749520	AT3G18930.3	41.558	77	43	2	130	204	125	201	3.75e-13	68.6
Msa0749520	AT3G18930.1	41.558	77	43	2	130	204	125	201	3.75e-13	68.6
Msa0749520	AT3G48030.1	46.250	80	34	3	131	209	186	257	5.18e-13	68.2
Msa0749520	AT4G28890.1	43.421	76	33	3	131	205	103	169	5.74e-13	68.2
Msa0749520	AT3G48030.2	46.250	80	34	3	131	209	108	179	6.81e-13	67.0

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Find 77 sgRNAs with CRISPR-Local

Find 228 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGGTGCCGCGAGAAATTG+AGG	0.516065	5_2:-3047822	None:intergenic
AAATAGGAAACGTGAGCCTC+CGG	0.500001	5_2:+3048080	Msa0749520:CDS
AACGAAGCATGTCGTTGTTG+CGG	0.577997	5_2:-3047904	None:intergenic
AACGGATTCGAGATCGGTGG+CGG	0.526950	5_2:-3048110	None:intergenic
AATCATTGGTGTTGTGCATT+TGG	0.374563	5_2:-3046823	None:intergenic
AATGACCAAGAGGTCGGTGT+TGG	0.552990	5_2:-3048023	None:intergenic
ACAGTCATCGTCGACATCGC+CGG	0.580449	5_2:-3047861	None:intergenic
ACCACCACGGACGACTGTTA+CGG	0.525467	5_2:+3047757	Msa0749520:CDS
ACGGTGATACTGTCAAACGG+AGG	0.799114	5_2:-3048055	None:intergenic
ACTCTTACTGCATAAGCAGA+TGG	0.563965	5_2:+3047951	Msa0749520:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TACTTCTTCAAAGCTGATAT+TGG	-	chr5_2:3046736-3046755	None:intergenic	30.0%
GGTTTAATAAAGAACTCGTA+TGG	+	chr5_2:3046978-3046997	Msa0749520:intron	30.0%
AATTTCAGATGTATAGTGGT+TGG	-	chr5_2:3047013-3047032	None:intergenic	30.0%
CATGAAAAATAACCAGACAA+TGG	+	chr5_2:3047034-3047053	Msa0749520:intron	30.0%
TGATATGTCCGTATCTATAT+TGG	-	chr5_2:3047095-3047114	None:intergenic	30.0%
TACTTCTTCAAAGCTGATAT+TGG	-	chr5_2:3046736-3046755	None:intergenic	30.0%
GGTTTAATAAAGAACTCGTA+TGG	+	chr5_2:3046978-3046997	Msa0749520:intron	30.0%
AATTTCAGATGTATAGTGGT+TGG	-	chr5_2:3047013-3047032	None:intergenic	30.0%
CATGAAAAATAACCAGACAA+TGG	+	chr5_2:3047034-3047053	Msa0749520:intron	30.0%
TGATATGTCCGTATCTATAT+TGG	-	chr5_2:3047095-3047114	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_2	gene	3046686	3048135	3046686	ID=Msa0749520;Name=Msa0749520
chr5_2	mRNA	3046686	3048135	3046686	ID=Msa0749520-mRNA-1;Parent=Msa0749520;Name=Msa0749520-mRNA-1;_AED=0.70;_eAED=0.70;_QI=0\|0\|0\|0.66\|0\|0\|3\|0\|243
chr5_2	exon	3046686	3046690	3046686	ID=Msa0749520-mRNA-1:exon:1045;Parent=Msa0749520-mRNA-1
chr5_2	exon	3046719	3046908	3046719	ID=Msa0749520-mRNA-1:exon:1046;Parent=Msa0749520-mRNA-1
chr5_2	exon	3047599	3048135	3047599	ID=Msa0749520-mRNA-1:exon:1047;Parent=Msa0749520-mRNA-1
chr5_2	CDS	3046686	3046690	3046686	ID=Msa0749520-mRNA-1:cds;Parent=Msa0749520-mRNA-1
chr5_2	CDS	3046719	3046908	3046719	ID=Msa0749520-mRNA-1:cds;Parent=Msa0749520-mRNA-1
chr5_2	CDS	3047599	3048135	3047599	ID=Msa0749520-mRNA-1:cds;Parent=Msa0749520-mRNA-1

Gene Sequence

>Msa0749520

ATGAACTATGACCTCAGCACCAATATCAGCTTTGAAGAAGTACAACTTGCATGCTTTACCCAGCACCAATATCCCACAACCTCCTTAAGCACACCATTATCAAGTTCCACCAAATGCACAACACCAATGATTATTCTAGCATACAAGTTTGCTCGTGGAGAAACCAATCCATCCGTGGCCAACAATACTCAAGCTACACCACAACGCATTTCTTTCTCTCTCTCTAACCTCTTTTCGTTTCACTCCATGGATGCAAACCTCGTTGGCTTTCTCATTTTCCTCGCGCTTCTCTTCCTTTCACTCGTTTCACAATTGCTATGTTTCCTTCATCACCGCCGTTCCAACCCAACGCCACCACCACGGACGACTGTTACGGAAACATTGGTAATTATCGACCGACTCCCTCTCTTCCGTTTCTCCTCAATTTCTCGCGGCACCTTTTCCACCATCGGACCCACCGGCGATGTCGACGATGACTGTGCAATCTGTTTCGAACAATTCCGCAACAACGACATGCTTCGTTCTCTCCCATGTTTCCACGCTTTTCACTCTTACTGCATAAGCAGATGGCTCAACACGAATTTGTCTTGTCCTCTCTGCCGCGATGTCCTCTCCGATTCCAACACCGACCTCTTGGTCATTCTCCGCTCACCTCCGTTTGACAGTATCACCGTCCAAATAGGAAACGTGAGCCTCCGGCGCGAAACCGCCACCGATCTCGAATCCGTTTGA

Protein sequence

>Msa0749520

MNYDLSTNISFEEVQLACFTQHQYPTTSLSTPLSSSTKCTTPMIILAYKFARGETNPSVANNTQATPQRISFSLSNLFSFHSMDANLVGFLIFLALLFLSLVSQLLCFLHHRRSNPTPPPRTTVTETLVIIDRLPLFRFSSISRGTFSTIGPTGDVDDDCAICFEQFRNNDMLRSLPCFHAFHSYCISRWLNTNLSCPLCRDVLSDSNTDLLVILRSPPFDSITVQIGNVSLRRETATDLESV*