AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0186200


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0186200 MtrunA17_Chr3g0140601 95.726 117 5 0 1 117 1 117 3.75e-78 226
Msa0186200 MtrunA17_Chr1g0146471 70.085 117 33 2 1 117 1 115 7.78e-58 174
Msa0186200 MtrunA17_Chr4g0063331 56.790 81 35 0 27 107 21 101 2.34e-24 89.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0186200 AT5G25940.1 57.955 88 37 0 19 106 16 103 2.47e-21 82.4

Find 26 sgRNAs with CRISPR-Local

Find 112 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCTGCTGACAAAACAATCT+TGG 0.341283 2_1:+912051 Msa0186200:CDS
TCAGAGACTCTTCTAGCAGC+AGG 0.453559 2_1:-912093 None:intergenic
TTGCAGTTCGGATGATTCCA+TGG 0.467990 2_1:+911544 Msa0186200:CDS
AACATCTTCTGCTCTTCTGC+GGG 0.476450 2_1:-910911 None:intergenic
AGGTGTGCGTGCTGGATTCA+AGG 0.476456 2_1:+911228 Msa0186200:CDS
GGATTCAAGGCAGCCGGCAT+CGG 0.492095 2_1:+911241 Msa0186200:CDS
ATTCACAGTTGGTTGCAGTT+CGG 0.494528 2_1:+911532 Msa0186200:intron
AAACCTCAATTATACTGCTC+AGG 0.499730 2_1:+911575 Msa0186200:CDS
CGTGCTGGATTCAAGGCAGC+CGG 0.500054 2_1:+911235 Msa0186200:CDS
CACATGTCCCTCAGTTCTGA+TGG 0.505127 2_1:-910851 None:intergenic
TTGAGGTTTGCCTTTGCCCA+TGG 0.506394 2_1:-911561 None:intergenic
CAACATCTTCTGCTCTTCTG+CGG 0.519948 2_1:-910912 None:intergenic
AGGTCTAGTCAATGTACAGC+TGG 0.529239 2_1:+910935 Msa0186200:CDS
CAGGCGCTTATCATATCTGC+CGG 0.532856 2_1:+911594 Msa0186200:CDS
TATGCAGAAGGTGTGCGTGC+TGG 0.539033 2_1:+911220 Msa0186200:intron
GCGCCTGAGCAGTATAATTG+AGG 0.541209 2_1:-911578 None:intergenic
GGCAGCCGGCATCGGATGTG+TGG 0.545487 2_1:+911249 Msa0186200:CDS
CAGAGACTCTTCTAGCAGCA+GGG 0.568654 2_1:-912092 None:intergenic
ACTCGCCACACATCCGATGC+CGG 0.574386 2_1:-911254 None:intergenic
TGCAGTTCGGATGATTCCAT+GGG 0.575792 2_1:+911545 Msa0186200:CDS
CATCAGAACTGAGGGACATG+TGG 0.592369 2_1:+910852 Msa0186200:CDS
GGGAATTCCATCAGAACTGA+GGG 0.594629 2_1:+910844 Msa0186200:CDS
CAGAAGAGCAGAAGATGTTG+AGG 0.594698 2_1:+910915 Msa0186200:CDS
TCGGATGATTCCATGGGCAA+AGG 0.603271 2_1:+911551 Msa0186200:CDS
AAGATTACGTACAGTAGGCA+CGG 0.635926 2_1:-911278 None:intergenic
TGGGAATTCCATCAGAACTG+AGG 0.659138 2_1:+910843 Msa0186200:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACTAAAAATACATATTTTTT+TGG + chr2_1:911340-911359 Msa0186200:intron 10.0%
!!! ACTAAAAATACATATTTTTT+TGG + chr2_1:911340-911359 Msa0186200:intron 10.0%
!!! TAACAAACTTTAAGAAATTA+TGG + chr2_1:911366-911385 Msa0186200:intron 15.0%
!! AAAAAAACAGAAATTCTTTA+AGG - chr2_1:911445-911464 None:intergenic 15.0%
!! TTTACTCCTATAATTAAATT+TGG - chr2_1:911714-911733 None:intergenic 15.0%
!!! TGATTAATGAATTTTATCTA+AGG - chr2_1:911883-911902 None:intergenic 15.0%
!!! TTCTAATTTTGATAATATAG+TGG + chr2_1:911984-912003 Msa0186200:intron 15.0%
!!! TAACAAACTTTAAGAAATTA+TGG + chr2_1:911366-911385 Msa0186200:intron 15.0%
!! AAAAAAACAGAAATTCTTTA+AGG - chr2_1:911445-911464 None:intergenic 15.0%
!! TTTACTCCTATAATTAAATT+TGG - chr2_1:911714-911733 None:intergenic 15.0%
!!! TGATTAATGAATTTTATCTA+AGG - chr2_1:911883-911902 None:intergenic 15.0%
!!! TTCTAATTTTGATAATATAG+TGG + chr2_1:911984-912003 Msa0186200:intron 15.0%
!! GATGAATAAGAAAATTAAAC+TGG - chr2_1:910993-911012 None:intergenic 20.0%
!! CATATTAAGAAAATGACAAA+CGG - chr2_1:911204-911223 None:intergenic 20.0%
!!! TTTGATAATATAGTGGTTTT+TGG + chr2_1:911991-912010 Msa0186200:intron 20.0%
!! GATGAATAAGAAAATTAAAC+TGG - chr2_1:910993-911012 None:intergenic 20.0%
!! CATATTAAGAAAATGACAAA+CGG - chr2_1:911204-911223 None:intergenic 20.0%
!!! TTTGATAATATAGTGGTTTT+TGG + chr2_1:911991-912010 Msa0186200:intron 20.0%
!! ATTTTCATCAATCAAGTCAT+AGG + chr2_1:911089-911108 Msa0186200:intron 25.0%
!! TCATTTTCTTAATATGCAGA+AGG + chr2_1:911208-911227 Msa0186200:intron 25.0%
!! ATCTTTTATGGACAAAACTT+AGG + chr2_1:911296-911315 Msa0186200:intron 25.0%
! TATCTCAAATGTAACAGATA+AGG - chr2_1:911488-911507 None:intergenic 25.0%
! ATGCCACCAAATTTAATTAT+AGG + chr2_1:911705-911724 Msa0186200:intron 25.0%
! ACTCCTATAATTAAATTTGG+TGG - chr2_1:911711-911730 None:intergenic 25.0%
!!! TAGGAGTAAAAGTATCATTT+CGG + chr2_1:911724-911743 Msa0186200:intron 25.0%
! ATCACTTGAATCTTATTACT+TGG + chr2_1:911899-911918 Msa0186200:intron 25.0%
!! ATTTTCATCAATCAAGTCAT+AGG + chr2_1:911089-911108 Msa0186200:intron 25.0%
!! TCATTTTCTTAATATGCAGA+AGG + chr2_1:911208-911227 Msa0186200:intron 25.0%
!! ATCTTTTATGGACAAAACTT+AGG + chr2_1:911296-911315 Msa0186200:intron 25.0%
! TATCTCAAATGTAACAGATA+AGG - chr2_1:911488-911507 None:intergenic 25.0%
! ATGCCACCAAATTTAATTAT+AGG + chr2_1:911705-911724 Msa0186200:intron 25.0%
! ACTCCTATAATTAAATTTGG+TGG - chr2_1:911711-911730 None:intergenic 25.0%
!!! TAGGAGTAAAAGTATCATTT+CGG + chr2_1:911724-911743 Msa0186200:intron 25.0%
! ATCACTTGAATCTTATTACT+TGG + chr2_1:911899-911918 Msa0186200:intron 25.0%
!! CAGCAAACTTGTGTTTTTAT+TGG - chr2_1:910882-910901 None:intergenic 30.0%
CATAAAAGATTACGTACAGT+AGG - chr2_1:911286-911305 None:intergenic 30.0%
! CTACTGTACGTAATCTTTTA+TGG + chr2_1:911284-911303 Msa0186200:intron 30.0%
TTATGGATTTGAGAAAACCA+TGG + chr2_1:911383-911402 Msa0186200:intron 30.0%
!! AGCTTTAGAAAATTCACAGT+TGG + chr2_1:911521-911540 Msa0186200:intron 30.0%
!! CAGCAAACTTGTGTTTTTAT+TGG - chr2_1:910882-910901 None:intergenic 30.0%
CATAAAAGATTACGTACAGT+AGG - chr2_1:911286-911305 None:intergenic 30.0%
! CTACTGTACGTAATCTTTTA+TGG + chr2_1:911284-911303 Msa0186200:intron 30.0%
TTATGGATTTGAGAAAACCA+TGG + chr2_1:911383-911402 Msa0186200:intron 30.0%
!! AGCTTTAGAAAATTCACAGT+TGG + chr2_1:911521-911540 Msa0186200:intron 30.0%
TCAATCAAGTCATAGGTGAT+AGG + chr2_1:911096-911115 Msa0186200:intron 35.0%
CTTCTTAACTAAGCTATCCA+TGG - chr2_1:911403-911422 None:intergenic 35.0%
AAACCTCAATTATACTGCTC+AGG + chr2_1:911575-911594 Msa0186200:CDS 35.0%
!! AATTTAAGTTGAGATCGCAC+CGG - chr2_1:911616-911635 None:intergenic 35.0%
TAGCTAACGATTTGATTCCA+CGG + chr2_1:911775-911794 Msa0186200:intron 35.0%
TCAATCAAGTCATAGGTGAT+AGG + chr2_1:911096-911115 Msa0186200:intron 35.0%
CTTCTTAACTAAGCTATCCA+TGG - chr2_1:911403-911422 None:intergenic 35.0%
AAACCTCAATTATACTGCTC+AGG + chr2_1:911575-911594 Msa0186200:CDS 35.0%
!! AATTTAAGTTGAGATCGCAC+CGG - chr2_1:911616-911635 None:intergenic 35.0%
TAGCTAACGATTTGATTCCA+CGG + chr2_1:911775-911794 Msa0186200:intron 35.0%
CATAGGTGATAGGTATAGTG+TGG + chr2_1:911106-911125 Msa0186200:intron 40.0%
AAGATTACGTACAGTAGGCA+CGG - chr2_1:911281-911300 None:intergenic 40.0%
ATTCACAGTTGGTTGCAGTT+CGG + chr2_1:911532-911551 Msa0186200:intron 40.0%
GACTCAACTAATCGAGAGTT+TGG + chr2_1:911806-911825 Msa0186200:intron 40.0%
CAACTAATCGAGAGTTTGGT+TGG + chr2_1:911810-911829 Msa0186200:intron 40.0%
CATAGGTGATAGGTATAGTG+TGG + chr2_1:911106-911125 Msa0186200:intron 40.0%
AAGATTACGTACAGTAGGCA+CGG - chr2_1:911281-911300 None:intergenic 40.0%
ATTCACAGTTGGTTGCAGTT+CGG + chr2_1:911532-911551 Msa0186200:intron 40.0%
GACTCAACTAATCGAGAGTT+TGG + chr2_1:911806-911825 Msa0186200:intron 40.0%
CAACTAATCGAGAGTTTGGT+TGG + chr2_1:911810-911829 Msa0186200:intron 40.0%
TGGGAATTCCATCAGAACTG+AGG + chr2_1:910843-910862 Msa0186200:CDS 45.0%
GGGAATTCCATCAGAACTGA+GGG + chr2_1:910844-910863 Msa0186200:CDS 45.0%
AACATCTTCTGCTCTTCTGC+GGG - chr2_1:910914-910933 None:intergenic 45.0%
CAACATCTTCTGCTCTTCTG+CGG - chr2_1:910915-910934 None:intergenic 45.0%
CAGAAGAGCAGAAGATGTTG+AGG + chr2_1:910915-910934 Msa0186200:CDS 45.0%
AGGTCTAGTCAATGTACAGC+TGG + chr2_1:910935-910954 Msa0186200:CDS 45.0%
TTGCAGTTCGGATGATTCCA+TGG + chr2_1:911544-911563 Msa0186200:CDS 45.0%
TGCAGTTCGGATGATTCCAT+GGG + chr2_1:911545-911564 Msa0186200:CDS 45.0%
CGCTGCTGACAAAACAATCT+TGG + chr2_1:912051-912070 Msa0186200:CDS 45.0%
TGGGAATTCCATCAGAACTG+AGG + chr2_1:910843-910862 Msa0186200:CDS 45.0%
GGGAATTCCATCAGAACTGA+GGG + chr2_1:910844-910863 Msa0186200:CDS 45.0%
AACATCTTCTGCTCTTCTGC+GGG - chr2_1:910914-910933 None:intergenic 45.0%
CAACATCTTCTGCTCTTCTG+CGG - chr2_1:910915-910934 None:intergenic 45.0%
CAGAAGAGCAGAAGATGTTG+AGG + chr2_1:910915-910934 Msa0186200:CDS 45.0%
AGGTCTAGTCAATGTACAGC+TGG + chr2_1:910935-910954 Msa0186200:CDS 45.0%
TTGCAGTTCGGATGATTCCA+TGG + chr2_1:911544-911563 Msa0186200:CDS 45.0%
TGCAGTTCGGATGATTCCAT+GGG + chr2_1:911545-911564 Msa0186200:CDS 45.0%
CGCTGCTGACAAAACAATCT+TGG + chr2_1:912051-912070 Msa0186200:CDS 45.0%
! CACATGTCCCTCAGTTCTGA+TGG - chr2_1:910854-910873 None:intergenic 50.0%
CATCAGAACTGAGGGACATG+TGG + chr2_1:910852-910871 Msa0186200:CDS 50.0%
TCGGATGATTCCATGGGCAA+AGG + chr2_1:911551-911570 Msa0186200:CDS 50.0%
TTGAGGTTTGCCTTTGCCCA+TGG - chr2_1:911564-911583 None:intergenic 50.0%
GCGCCTGAGCAGTATAATTG+AGG - chr2_1:911581-911600 None:intergenic 50.0%
CAGGCGCTTATCATATCTGC+CGG + chr2_1:911594-911613 Msa0186200:CDS 50.0%
AGTTGAGTCTGTATCGTCCG+TGG - chr2_1:911795-911814 None:intergenic 50.0%
CAGAGACTCTTCTAGCAGCA+GGG - chr2_1:912095-912114 None:intergenic 50.0%
TCAGAGACTCTTCTAGCAGC+AGG - chr2_1:912096-912115 None:intergenic 50.0%
! CACATGTCCCTCAGTTCTGA+TGG - chr2_1:910854-910873 None:intergenic 50.0%
CATCAGAACTGAGGGACATG+TGG + chr2_1:910852-910871 Msa0186200:CDS 50.0%
TCGGATGATTCCATGGGCAA+AGG + chr2_1:911551-911570 Msa0186200:CDS 50.0%
TTGAGGTTTGCCTTTGCCCA+TGG - chr2_1:911564-911583 None:intergenic 50.0%
GCGCCTGAGCAGTATAATTG+AGG - chr2_1:911581-911600 None:intergenic 50.0%
CAGGCGCTTATCATATCTGC+CGG + chr2_1:911594-911613 Msa0186200:CDS 50.0%
AGTTGAGTCTGTATCGTCCG+TGG - chr2_1:911795-911814 None:intergenic 50.0%
CAGAGACTCTTCTAGCAGCA+GGG - chr2_1:912095-912114 None:intergenic 50.0%
TCAGAGACTCTTCTAGCAGC+AGG - chr2_1:912096-912115 None:intergenic 50.0%
! TATGCAGAAGGTGTGCGTGC+TGG + chr2_1:911220-911239 Msa0186200:intron 55.0%
AGGTGTGCGTGCTGGATTCA+AGG + chr2_1:911228-911247 Msa0186200:CDS 55.0%
! TATGCAGAAGGTGTGCGTGC+TGG + chr2_1:911220-911239 Msa0186200:intron 55.0%
AGGTGTGCGTGCTGGATTCA+AGG + chr2_1:911228-911247 Msa0186200:CDS 55.0%
CGTGCTGGATTCAAGGCAGC+CGG + chr2_1:911235-911254 Msa0186200:CDS 60.0%
GGATTCAAGGCAGCCGGCAT+CGG + chr2_1:911241-911260 Msa0186200:CDS 60.0%
ACTCGCCACACATCCGATGC+CGG - chr2_1:911257-911276 None:intergenic 60.0%
CGTGCTGGATTCAAGGCAGC+CGG + chr2_1:911235-911254 Msa0186200:CDS 60.0%
GGATTCAAGGCAGCCGGCAT+CGG + chr2_1:911241-911260 Msa0186200:CDS 60.0%
ACTCGCCACACATCCGATGC+CGG - chr2_1:911257-911276 None:intergenic 60.0%
! GGCAGCCGGCATCGGATGTG+TGG + chr2_1:911249-911268 Msa0186200:CDS 70.0%
! GGCAGCCGGCATCGGATGTG+TGG + chr2_1:911249-911268 Msa0186200:CDS 70.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 910842 912129 910842 ID=Msa0186200;Name=Msa0186200
chr2_1 mRNA 910842 912129 910842 ID=Msa0186200-mRNA-1;Parent=Msa0186200;Name=Msa0186200-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0|0.66|0.25|1|1|1|4|0|117
chr2_1 exon 910842 910956 910842 ID=Msa0186200-mRNA-1:exon:214;Parent=Msa0186200-mRNA-1
chr2_1 exon 911227 911288 911227 ID=Msa0186200-mRNA-1:exon:215;Parent=Msa0186200-mRNA-1
chr2_1 exon 911540 911615 911540 ID=Msa0186200-mRNA-1:exon:216;Parent=Msa0186200-mRNA-1
chr2_1 exon 912029 912129 912029 ID=Msa0186200-mRNA-1:exon:217;Parent=Msa0186200-mRNA-1
chr2_1 CDS 910842 910956 910842 ID=Msa0186200-mRNA-1:cds;Parent=Msa0186200-mRNA-1
chr2_1 CDS 911227 911288 911227 ID=Msa0186200-mRNA-1:cds;Parent=Msa0186200-mRNA-1
chr2_1 CDS 911540 911615 911540 ID=Msa0186200-mRNA-1:cds;Parent=Msa0186200-mRNA-1
chr2_1 CDS 912029 912129 912029 ID=Msa0186200-mRNA-1:cds;Parent=Msa0186200-mRNA-1
Gene Sequence

>Msa0186200

ATGGGAATTCCATCAGAACTGAGGGACATGTGGATTGCCAATAAAAACACAAGTTTGCTGATTGCTTCTCCCGCAGAAGAGCAGAAGATGTTGAGGTCTAGTCAATGTACAGCTGAAGGTGTGCGTGCTGGATTCAAGGCAGCCGGCATCGGATGTGTGGCGAGTACCGTGCCTACTTTGGTTGCAGTTCGGATGATTCCATGGGCAAAGGCAAACCTCAATTATACTGCTCAGGCGCTTATCATATCTGCCGTATCTGTTGCGTCATTCTTTGTCGCTGCTGACAAAACAATCTTGGCTTGTGCAAGAAAACAATCCCTGCTGCTAGAAGAGTCTCTGAAACATGAAAGATGA

Protein sequence

>Msa0186200

MGIPSELRDMWIANKNTSLLIASPAEEQKMLRSSQCTAEGVRAGFKAAGIGCVASTVPTLVAVRMIPWAKANLNYTAQALIISAVSVASFFVAADKTILACARKQSLLLEESLKHER*