AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0522280


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0522280 MtrunA17_Chr3g0140601 95.726 117 5 0 1 117 1 117 3.75e-78 226
Msa0522280 MtrunA17_Chr1g0146471 70.085 117 33 2 1 117 1 115 7.78e-58 174
Msa0522280 MtrunA17_Chr4g0063331 56.790 81 35 0 27 107 21 101 2.34e-24 89.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0522280 AT5G25940.1 57.955 88 37 0 19 106 16 103 2.47e-21 82.4

Find 26 sgRNAs with CRISPR-Local

Find 114 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCTGCTGACAAAACAATCT+TGG 0.341283 3_4:-75348023 Msa0522280:CDS
TCAGAGACTCTTCTAGCAGC+AGG 0.453559 3_4:+75347981 None:intergenic
TTGCAGTTCGGATGATTCCA+TGG 0.467990 3_4:-75348530 Msa0522280:CDS
AACATCTTCTGCTCTTCTGC+GGG 0.476450 3_4:+75349170 None:intergenic
AGGTGTGCGTGCTGGATTCA+AGG 0.476456 3_4:-75348849 Msa0522280:CDS
GGATTCAAGGCAGCCGGCAT+CGG 0.492095 3_4:-75348836 Msa0522280:CDS
ATTCACAGTTGGTTGCAGTT+CGG 0.494528 3_4:-75348542 Msa0522280:intron
AAACCTCAATTATACTGCTC+AGG 0.499730 3_4:-75348499 Msa0522280:CDS
CGTGCTGGATTCAAGGCAGC+CGG 0.500054 3_4:-75348842 Msa0522280:CDS
CACATGTCCCTCAGTTCTGA+TGG 0.505127 3_4:+75349230 None:intergenic
TTGAGGTTTGCCTTTGCCCA+TGG 0.506394 3_4:+75348513 None:intergenic
CAACATCTTCTGCTCTTCTG+CGG 0.519948 3_4:+75349169 None:intergenic
AGGTCTAGTCAATGTACAGC+TGG 0.529239 3_4:-75349146 Msa0522280:intron
CAGGCGCTTATCATATCTGC+CGG 0.532856 3_4:-75348480 Msa0522280:intron
TATGCAGAAGGTGTGCGTGC+TGG 0.539033 3_4:-75348857 Msa0522280:intron
GCGCCTGAGCAGTATAATTG+AGG 0.541209 3_4:+75348496 None:intergenic
GGCAGCCGGCATCGGATGTG+TGG 0.545487 3_4:-75348828 Msa0522280:CDS
CAGAGACTCTTCTAGCAGCA+GGG 0.568654 3_4:+75347982 None:intergenic
ACTCGCCACACATCCGATGC+CGG 0.574386 3_4:+75348823 None:intergenic
TGCAGTTCGGATGATTCCAT+GGG 0.575792 3_4:-75348529 Msa0522280:CDS
CATCAGAACTGAGGGACATG+TGG 0.592369 3_4:-75349229 Msa0522280:CDS
GGGAATTCCATCAGAACTGA+GGG 0.594629 3_4:-75349237 Msa0522280:CDS
CAGAAGAGCAGAAGATGTTG+AGG 0.594698 3_4:-75349166 Msa0522280:CDS
TCGGATGATTCCATGGGCAA+AGG 0.603271 3_4:-75348523 Msa0522280:CDS
AAGATTACGTACAGTAGGCA+CGG 0.635926 3_4:+75348799 None:intergenic
TGGGAATTCCATCAGAACTG+AGG 0.659138 3_4:-75349238 Msa0522280:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACTAAAAATACATATTTTTT+TGG - chr3_4:75348470-75348489 Msa0522280:intron 10.0%
!!! ACTAAAAATACATATTTTTT+TGG - chr3_4:75348470-75348489 Msa0522280:intron 10.0%
!! ATGAATAAGAAAATTAAAAC+TGG + chr3_4:75348118-75348137 None:intergenic 15.0%
!!! AACAAACTTTAAGAAAATTA+TGG - chr3_4:75348497-75348516 Msa0522280:CDS 15.0%
!! AAAAAAACAGAAATTCTTTA+AGG + chr3_4:75348576-75348595 None:intergenic 15.0%
!! TTTACTCCTATAATTAAATT+TGG + chr3_4:75348846-75348865 None:intergenic 15.0%
!!! TGATTAATGAATTTTATCTA+AGG + chr3_4:75349015-75349034 None:intergenic 15.0%
!!! TTCTAATTTTGATAATATAG+TGG - chr3_4:75349116-75349135 Msa0522280:intron 15.0%
!! ATGAATAAGAAAATTAAAAC+TGG + chr3_4:75348118-75348137 None:intergenic 15.0%
!!! AACAAACTTTAAGAAAATTA+TGG - chr3_4:75348497-75348516 Msa0522280:CDS 15.0%
!! AAAAAAACAGAAATTCTTTA+AGG + chr3_4:75348576-75348595 None:intergenic 15.0%
!! TTTACTCCTATAATTAAATT+TGG + chr3_4:75348846-75348865 None:intergenic 15.0%
!!! TGATTAATGAATTTTATCTA+AGG + chr3_4:75349015-75349034 None:intergenic 15.0%
!!! TTCTAATTTTGATAATATAG+TGG - chr3_4:75349116-75349135 Msa0522280:intron 15.0%
!! CATATTAAGAAAATGACAAA+CGG + chr3_4:75348333-75348352 None:intergenic 20.0%
!!! TTTGATAATATAGTGGTTTT+TGG - chr3_4:75349123-75349142 Msa0522280:intron 20.0%
!! CATATTAAGAAAATGACAAA+CGG + chr3_4:75348333-75348352 None:intergenic 20.0%
!!! TTTGATAATATAGTGGTTTT+TGG - chr3_4:75349123-75349142 Msa0522280:intron 20.0%
!! ATTTTCATCAATCAAGTCAT+AGG - chr3_4:75348218-75348237 Msa0522280:intron 25.0%
!! TCATTTTCTTAATATGCAGA+AGG - chr3_4:75348337-75348356 Msa0522280:intron 25.0%
! ATTAAAAGATTACGTACAGT+AGG + chr3_4:75348415-75348434 None:intergenic 25.0%
!! TACTGTACGTAATCTTTTAA+TGG - chr3_4:75348414-75348433 Msa0522280:intron 25.0%
!!! TCTTTTAATGGACAAAACTT+AGG - chr3_4:75348426-75348445 Msa0522280:intron 25.0%
! TATCTCAAATGTAACAGATA+AGG + chr3_4:75348619-75348638 None:intergenic 25.0%
! ATGCCACCAAATTTAATTAT+AGG - chr3_4:75348837-75348856 Msa0522280:CDS 25.0%
! ACTCCTATAATTAAATTTGG+TGG + chr3_4:75348843-75348862 None:intergenic 25.0%
!!! TAGGAGTAAAAGTATCATTT+CGG - chr3_4:75348856-75348875 Msa0522280:intron 25.0%
! ATCACTTGAATCTTATTACT+TGG - chr3_4:75349031-75349050 Msa0522280:intron 25.0%
!! ATTTTCATCAATCAAGTCAT+AGG - chr3_4:75348218-75348237 Msa0522280:intron 25.0%
!! TCATTTTCTTAATATGCAGA+AGG - chr3_4:75348337-75348356 Msa0522280:intron 25.0%
! ATTAAAAGATTACGTACAGT+AGG + chr3_4:75348415-75348434 None:intergenic 25.0%
!! TACTGTACGTAATCTTTTAA+TGG - chr3_4:75348414-75348433 Msa0522280:intron 25.0%
!!! TCTTTTAATGGACAAAACTT+AGG - chr3_4:75348426-75348445 Msa0522280:intron 25.0%
! TATCTCAAATGTAACAGATA+AGG + chr3_4:75348619-75348638 None:intergenic 25.0%
! ATGCCACCAAATTTAATTAT+AGG - chr3_4:75348837-75348856 Msa0522280:CDS 25.0%
! ACTCCTATAATTAAATTTGG+TGG + chr3_4:75348843-75348862 None:intergenic 25.0%
!!! TAGGAGTAAAAGTATCATTT+CGG - chr3_4:75348856-75348875 Msa0522280:intron 25.0%
! ATCACTTGAATCTTATTACT+TGG - chr3_4:75349031-75349050 Msa0522280:intron 25.0%
!! CAGCAAACTTGTGTTTTTAT+TGG + chr3_4:75348007-75348026 None:intergenic 30.0%
GACTTGATTGATGAAAATCT+TGG + chr3_4:75348216-75348235 None:intergenic 30.0%
TTATGGATTTGAGAAAACCA+TGG - chr3_4:75348514-75348533 Msa0522280:CDS 30.0%
!! AGCTTTAGAAAATTCACAGT+TGG - chr3_4:75348653-75348672 Msa0522280:intron 30.0%
!! CAGCAAACTTGTGTTTTTAT+TGG + chr3_4:75348007-75348026 None:intergenic 30.0%
GACTTGATTGATGAAAATCT+TGG + chr3_4:75348216-75348235 None:intergenic 30.0%
TTATGGATTTGAGAAAACCA+TGG - chr3_4:75348514-75348533 Msa0522280:CDS 30.0%
!! AGCTTTAGAAAATTCACAGT+TGG - chr3_4:75348653-75348672 Msa0522280:intron 30.0%
TCAATCAAGTCATAGGTGAT+AGG - chr3_4:75348225-75348244 Msa0522280:intron 35.0%
CTTCTTAACTAAGCTATCCA+TGG + chr3_4:75348534-75348553 None:intergenic 35.0%
AAACCTCAATTATACTGCTC+AGG - chr3_4:75348707-75348726 Msa0522280:intron 35.0%
!! AATTTAAGTTGAGATCGCAC+CGG + chr3_4:75348748-75348767 None:intergenic 35.0%
TAGCTAACGATTTGATTCCA+CGG - chr3_4:75348907-75348926 Msa0522280:intron 35.0%
TCAATCAAGTCATAGGTGAT+AGG - chr3_4:75348225-75348244 Msa0522280:intron 35.0%
CTTCTTAACTAAGCTATCCA+TGG + chr3_4:75348534-75348553 None:intergenic 35.0%
AAACCTCAATTATACTGCTC+AGG - chr3_4:75348707-75348726 Msa0522280:intron 35.0%
!! AATTTAAGTTGAGATCGCAC+CGG + chr3_4:75348748-75348767 None:intergenic 35.0%
TAGCTAACGATTTGATTCCA+CGG - chr3_4:75348907-75348926 Msa0522280:intron 35.0%
CATAGGTGATAGGTATAGTG+TGG - chr3_4:75348235-75348254 Msa0522280:intron 40.0%
AAGATTACGTACAGTAGGCA+CGG + chr3_4:75348410-75348429 None:intergenic 40.0%
ATTCACAGTTGGTTGCAGTT+CGG - chr3_4:75348664-75348683 Msa0522280:intron 40.0%
GACTCAACTAATCGAGAGTT+TGG - chr3_4:75348938-75348957 Msa0522280:intron 40.0%
CAACTAATCGAGAGTTTGGT+TGG - chr3_4:75348942-75348961 Msa0522280:intron 40.0%
CATAGGTGATAGGTATAGTG+TGG - chr3_4:75348235-75348254 Msa0522280:intron 40.0%
AAGATTACGTACAGTAGGCA+CGG + chr3_4:75348410-75348429 None:intergenic 40.0%
ATTCACAGTTGGTTGCAGTT+CGG - chr3_4:75348664-75348683 Msa0522280:intron 40.0%
GACTCAACTAATCGAGAGTT+TGG - chr3_4:75348938-75348957 Msa0522280:intron 40.0%
CAACTAATCGAGAGTTTGGT+TGG - chr3_4:75348942-75348961 Msa0522280:intron 40.0%
TGGGAATTCCATCAGAACTG+AGG - chr3_4:75347968-75347987 Msa0522280:CDS 45.0%
GGGAATTCCATCAGAACTGA+GGG - chr3_4:75347969-75347988 Msa0522280:CDS 45.0%
AACATCTTCTGCTCTTCTGC+GGG + chr3_4:75348039-75348058 None:intergenic 45.0%
CAACATCTTCTGCTCTTCTG+CGG + chr3_4:75348040-75348059 None:intergenic 45.0%
CAGAAGAGCAGAAGATGTTG+AGG - chr3_4:75348040-75348059 Msa0522280:CDS 45.0%
AGGTCTAGTCAATGTACAGC+TGG - chr3_4:75348060-75348079 Msa0522280:intron 45.0%
TTGCAGTTCGGATGATTCCA+TGG - chr3_4:75348676-75348695 Msa0522280:intron 45.0%
TGCAGTTCGGATGATTCCAT+GGG - chr3_4:75348677-75348696 Msa0522280:intron 45.0%
CGCTGCTGACAAAACAATCT+TGG - chr3_4:75349183-75349202 Msa0522280:CDS 45.0%
TGGGAATTCCATCAGAACTG+AGG - chr3_4:75347968-75347987 Msa0522280:CDS 45.0%
GGGAATTCCATCAGAACTGA+GGG - chr3_4:75347969-75347988 Msa0522280:CDS 45.0%
AACATCTTCTGCTCTTCTGC+GGG + chr3_4:75348039-75348058 None:intergenic 45.0%
CAACATCTTCTGCTCTTCTG+CGG + chr3_4:75348040-75348059 None:intergenic 45.0%
CAGAAGAGCAGAAGATGTTG+AGG - chr3_4:75348040-75348059 Msa0522280:CDS 45.0%
AGGTCTAGTCAATGTACAGC+TGG - chr3_4:75348060-75348079 Msa0522280:intron 45.0%
TTGCAGTTCGGATGATTCCA+TGG - chr3_4:75348676-75348695 Msa0522280:intron 45.0%
TGCAGTTCGGATGATTCCAT+GGG - chr3_4:75348677-75348696 Msa0522280:intron 45.0%
CGCTGCTGACAAAACAATCT+TGG - chr3_4:75349183-75349202 Msa0522280:CDS 45.0%
! CACATGTCCCTCAGTTCTGA+TGG + chr3_4:75347979-75347998 None:intergenic 50.0%
CATCAGAACTGAGGGACATG+TGG - chr3_4:75347977-75347996 Msa0522280:CDS 50.0%
TCGGATGATTCCATGGGCAA+AGG - chr3_4:75348683-75348702 Msa0522280:intron 50.0%
TTGAGGTTTGCCTTTGCCCA+TGG + chr3_4:75348696-75348715 None:intergenic 50.0%
GCGCCTGAGCAGTATAATTG+AGG + chr3_4:75348713-75348732 None:intergenic 50.0%
CAGGCGCTTATCATATCTGC+CGG - chr3_4:75348726-75348745 Msa0522280:intron 50.0%
AGTTGAGTCTGTATCGTCCG+TGG + chr3_4:75348927-75348946 None:intergenic 50.0%
CAGAGACTCTTCTAGCAGCA+GGG + chr3_4:75349227-75349246 None:intergenic 50.0%
TCAGAGACTCTTCTAGCAGC+AGG + chr3_4:75349228-75349247 None:intergenic 50.0%
! CACATGTCCCTCAGTTCTGA+TGG + chr3_4:75347979-75347998 None:intergenic 50.0%
CATCAGAACTGAGGGACATG+TGG - chr3_4:75347977-75347996 Msa0522280:CDS 50.0%
TCGGATGATTCCATGGGCAA+AGG - chr3_4:75348683-75348702 Msa0522280:intron 50.0%
TTGAGGTTTGCCTTTGCCCA+TGG + chr3_4:75348696-75348715 None:intergenic 50.0%
GCGCCTGAGCAGTATAATTG+AGG + chr3_4:75348713-75348732 None:intergenic 50.0%
CAGGCGCTTATCATATCTGC+CGG - chr3_4:75348726-75348745 Msa0522280:intron 50.0%
AGTTGAGTCTGTATCGTCCG+TGG + chr3_4:75348927-75348946 None:intergenic 50.0%
CAGAGACTCTTCTAGCAGCA+GGG + chr3_4:75349227-75349246 None:intergenic 50.0%
TCAGAGACTCTTCTAGCAGC+AGG + chr3_4:75349228-75349247 None:intergenic 50.0%
! TATGCAGAAGGTGTGCGTGC+TGG - chr3_4:75348349-75348368 Msa0522280:intron 55.0%
AGGTGTGCGTGCTGGATTCA+AGG - chr3_4:75348357-75348376 Msa0522280:intron 55.0%
! TATGCAGAAGGTGTGCGTGC+TGG - chr3_4:75348349-75348368 Msa0522280:intron 55.0%
AGGTGTGCGTGCTGGATTCA+AGG - chr3_4:75348357-75348376 Msa0522280:intron 55.0%
CGTGCTGGATTCAAGGCAGC+CGG - chr3_4:75348364-75348383 Msa0522280:intron 60.0%
GGATTCAAGGCAGCCGGCAT+CGG - chr3_4:75348370-75348389 Msa0522280:intron 60.0%
ACTCGCCACACATCCGATGC+CGG + chr3_4:75348386-75348405 None:intergenic 60.0%
CGTGCTGGATTCAAGGCAGC+CGG - chr3_4:75348364-75348383 Msa0522280:intron 60.0%
GGATTCAAGGCAGCCGGCAT+CGG - chr3_4:75348370-75348389 Msa0522280:intron 60.0%
ACTCGCCACACATCCGATGC+CGG + chr3_4:75348386-75348405 None:intergenic 60.0%
! GGCAGCCGGCATCGGATGTG+TGG - chr3_4:75348378-75348397 Msa0522280:intron 70.0%
! GGCAGCCGGCATCGGATGTG+TGG - chr3_4:75348378-75348397 Msa0522280:intron 70.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 75347967 75349261 75347967 ID=Msa0522280;Name=Msa0522280
chr3_4 mRNA 75347967 75349261 75347967 ID=Msa0522280-mRNA-1;Parent=Msa0522280;Name=Msa0522280-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0|0.66|0.25|1|1|1|4|0|117
chr3_4 exon 75349147 75349261 75349147 ID=Msa0522280-mRNA-1:exon:21574;Parent=Msa0522280-mRNA-1
chr3_4 exon 75348811 75348872 75348811 ID=Msa0522280-mRNA-1:exon:21573;Parent=Msa0522280-mRNA-1
chr3_4 exon 75348481 75348556 75348481 ID=Msa0522280-mRNA-1:exon:21572;Parent=Msa0522280-mRNA-1
chr3_4 exon 75347967 75348067 75347967 ID=Msa0522280-mRNA-1:exon:21571;Parent=Msa0522280-mRNA-1
chr3_4 CDS 75349147 75349261 75349147 ID=Msa0522280-mRNA-1:cds;Parent=Msa0522280-mRNA-1
chr3_4 CDS 75348811 75348872 75348811 ID=Msa0522280-mRNA-1:cds;Parent=Msa0522280-mRNA-1
chr3_4 CDS 75348481 75348556 75348481 ID=Msa0522280-mRNA-1:cds;Parent=Msa0522280-mRNA-1
chr3_4 CDS 75347967 75348067 75347967 ID=Msa0522280-mRNA-1:cds;Parent=Msa0522280-mRNA-1
Gene Sequence

>Msa0522280

ATGGGAATTCCATCAGAACTGAGGGACATGTGGATTGCCAATAAAAACACAAGTTTGCTGATTGCTTCTCCCGCAGAAGAGCAGAAGATGTTGAGGTCTAGTCAATGTACAGCTGAAGGTGTGCGTGCTGGATTCAAGGCAGCCGGCATCGGATGTGTGGCGAGTACCGTGCCTACTTTGGTTGCAGTTCGGATGATTCCATGGGCAAAGGCAAACCTCAATTATACTGCTCAGGCGCTTATCATATCTGCCGTATCTGTTGCGTCATTCTTTGTCGCTGCTGACAAAACAATCTTGGCTTGTGCAAGAAAACAATCCCTGCTGCTAGAAGAGTCTCTGAAACATGAAAGATGA

Protein sequence

>Msa0522280

MGIPSELRDMWIANKNTSLLIASPAEEQKMLRSSQCTAEGVRAGFKAAGIGCVASTVPTLVAVRMIPWAKANLNYTAQALIISAVSVASFFVAADKTILACARKQSLLLEESLKHER*