Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0804930 | XP_003613125.2 | 90.707 | 721 | 16 | 3 | 76 | 765 | 1 | 701 | 0.0 | 1263 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0804930 | sp|Q8RY46|AB26B_ARATH | 66.201 | 716 | 184 | 4 | 79 | 764 | 12 | 699 | 0.0 | 948 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0000220 | Msa0804930 | 0.893455 | 6.185391e-75 | -8.615850e-47 |
| Msa0001460 | Msa0804930 | 0.822850 | 1.900054e-53 | -8.615850e-47 |
| Msa0002060 | Msa0804930 | 0.833292 | 5.798512e-56 | -8.615850e-47 |
| Msa0003370 | Msa0804930 | 0.810330 | 1.216483e-50 | -8.615850e-47 |
| Msa0003470 | Msa0804930 | 0.856891 | 2.366635e-62 | -8.615850e-47 |
| Msa0005160 | Msa0804930 | 0.850643 | 1.487044e-60 | -8.615850e-47 |
| Msa0005690 | Msa0804930 | 0.800472 | 1.418103e-48 | -8.615850e-47 |
| Msa0006700 | Msa0804930 | 0.801393 | 9.198444e-49 | -8.615850e-47 |
| Msa0006820 | Msa0804930 | 0.813497 | 2.484153e-51 | -8.615850e-47 |
| Msa0007160 | Msa0804930 | 0.882425 | 1.052930e-70 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0804930 | MtrunA17_Chr5g0412741 | 90.707 | 721 | 16 | 3 | 76 | 765 | 1 | 701 | 0.0 | 1263 |
| Msa0804930 | MtrunA17_Chr7g0227391 | 54.185 | 681 | 253 | 6 | 113 | 764 | 18 | 668 | 0.0 | 731 |
| Msa0804930 | MtrunA17_Chr4g0061601 | 37.743 | 514 | 280 | 5 | 253 | 757 | 140 | 622 | 4.77e-100 | 322 |
| Msa0804930 | MtrunA17_Chr4g0069361 | 34.034 | 523 | 288 | 12 | 253 | 756 | 786 | 1270 | 1.93e-74 | 263 |
| Msa0804930 | MtrunA17_Chr4g0069361 | 34.489 | 519 | 294 | 12 | 255 | 760 | 131 | 616 | 2.58e-71 | 254 |
| Msa0804930 | MtrunA17_Chr4g0041871 | 34.393 | 535 | 289 | 12 | 255 | 765 | 93 | 589 | 1.49e-73 | 260 |
| Msa0804930 | MtrunA17_Chr4g0041871 | 32.302 | 517 | 313 | 9 | 254 | 760 | 756 | 1245 | 9.16e-65 | 234 |
| Msa0804930 | MtrunA17_Chr6g0451911 | 34.638 | 511 | 286 | 11 | 254 | 749 | 765 | 1242 | 1.55e-73 | 260 |
| Msa0804930 | MtrunA17_Chr6g0451911 | 33.592 | 515 | 304 | 10 | 255 | 760 | 114 | 599 | 5.78e-68 | 244 |
| Msa0804930 | MtrunA17_Chr6g0481921 | 34.840 | 531 | 286 | 12 | 255 | 765 | 24 | 514 | 2.57e-73 | 259 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0804930 | AT1G70610.1 | 66.201 | 716 | 184 | 4 | 79 | 764 | 12 | 699 | 0.0 | 948 |
| Msa0804930 | AT1G70610.2 | 76.953 | 512 | 98 | 1 | 253 | 764 | 27 | 518 | 0.0 | 818 |
| Msa0804930 | AT5G39040.1 | 34.510 | 510 | 308 | 3 | 253 | 760 | 149 | 634 | 7.44e-87 | 288 |
| Msa0804930 | AT1G27940.1 | 36.699 | 515 | 287 | 11 | 255 | 760 | 124 | 608 | 4.69e-82 | 285 |
| Msa0804930 | AT1G27940.1 | 31.504 | 565 | 317 | 14 | 219 | 760 | 725 | 1242 | 3.08e-68 | 245 |
| Msa0804930 | AT1G28010.1 | 37.476 | 515 | 283 | 12 | 255 | 760 | 125 | 609 | 7.20e-80 | 279 |
| Msa0804930 | AT1G28010.1 | 33.205 | 518 | 303 | 10 | 255 | 760 | 758 | 1244 | 1.84e-66 | 240 |
| Msa0804930 | AT2G47000.6 | 33.721 | 516 | 304 | 9 | 253 | 758 | 792 | 1279 | 1.88e-74 | 264 |
| Msa0804930 | AT2G47000.6 | 33.852 | 514 | 308 | 8 | 255 | 762 | 137 | 624 | 5.04e-69 | 248 |
| Msa0804930 | AT2G47000.5 | 33.721 | 516 | 304 | 9 | 253 | 758 | 792 | 1279 | 1.88e-74 | 264 |
Find 204 sgRNAs with CRISPR-Local
Find 650 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATACGATTTGATAAGAA+GGG | 0.554184 | 5_3:-21307639 | Msa0804930:three_prime_UTR |
| AAAGCATACCAAACGGTAAC+GGG | 0.454848 | 5_3:+21314501 | None:intergenic |
| AAAGCGCTGCATATGGATTT+TGG | 0.200298 | 5_3:-21311474 | Msa0804930:CDS |
| AAATCAGGAGGATGTTGTGT+GGG | 0.659647 | 5_3:-21309066 | Msa0804930:CDS |
| AACGAGGAAGAAGATGATGA+TGG | 0.504680 | 5_3:+21314889 | None:intergenic |
| AACTCTACCTGACCGCTTGT+AGG | 0.455681 | 5_3:+21310334 | None:intergenic |
| AAGAAACTCGTTAGAAGCAT+TGG | 0.448929 | 5_3:+21314623 | None:intergenic |
| AAGAAGAGAGGGTGAAATTG+TGG | 0.535051 | 5_3:+21314727 | None:intergenic |
| AAGACTTGGATGTCATGTGG+TGG | 0.696777 | 5_3:-21309256 | Msa0804930:CDS |
| AAGAGAGGGTGAAATTGTGG+CGG | 0.653474 | 5_3:+21314730 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GAATCAATATCATACCCATT+TGG | + | chr5_3:21307448-21307467 | None:intergenic | 30.0% | |
| ATAAACAGCTAAAACCAAGA+AGG | + | chr5_3:21307725-21307744 | None:intergenic | 30.0% | |
| TCTTACTGGTGTTTAACAAT+CGG | + | chr5_3:21307830-21307849 | None:intergenic | 30.0% | |
| AAATCTTTGTGTCATATGAC+TGG | + | chr5_3:21307978-21307997 | None:intergenic | 30.0% | |
| GTTAACACAAACAAGAAAGT+AGG | - | chr5_3:21308300-21308319 | Msa0804930:intron | 30.0% | |
| TCGTGTAAATAAGTTCAGTT+TGG | - | chr5_3:21308511-21308530 | Msa0804930:intron | 30.0% | |
| ATCCAAAACCCTCAAAAAAT+AGG | - | chr5_3:21308688-21308707 | Msa0804930:intron | 30.0% | |
| TCCAAAACCCTCAAAAAATA+GGG | - | chr5_3:21308689-21308708 | Msa0804930:intron | 30.0% | |
| CGAAACTTGACCAAAATATT+AGG | - | chr5_3:21308959-21308978 | Msa0804930:CDS | 30.0% | |
| TAAAAAATGGGTCTAAACGA+TGG | + | chr5_3:21309060-21309079 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_3 | gene | 21307384 | 21317792 | 21307384 | ID=Msa0804930;Name=Msa0804930 |
| chr5_3 | mRNA | 21307384 | 21317792 | 21307384 | ID=Msa0804930-mRNA-1;Parent=Msa0804930;Name=Msa0804930-mRNA-1;_AED=0.15;_eAED=0.15;_QI=0|0.73|0.75|0.85|1|1|20|525|765 |
| chr5_3 | exon | 21307384 | 21307989 | 21307384 | ID=Msa0804930-mRNA-1:exon:8556;Parent=Msa0804930-mRNA-1 |
| chr5_3 | exon | 21308377 | 21308437 | 21308377 | ID=Msa0804930-mRNA-1:exon:8555;Parent=Msa0804930-mRNA-1 |
| chr5_3 | exon | 21308516 | 21308580 | 21308516 | ID=Msa0804930-mRNA-1:exon:8554;Parent=Msa0804930-mRNA-1 |
| chr5_3 | exon | 21308869 | 21309150 | 21308869 | ID=Msa0804930-mRNA-1:exon:8553;Parent=Msa0804930-mRNA-1 |
| chr5_3 | exon | 21309229 | 21309303 | 21309229 | ID=Msa0804930-mRNA-1:exon:8552;Parent=Msa0804930-mRNA-1 |
| chr5_3 | exon | 21309558 | 21309617 | 21309558 | ID=Msa0804930-mRNA-1:exon:8551;Parent=Msa0804930-mRNA-1 |
| chr5_3 | exon | 21310342 | 21310416 | 21310342 | ID=Msa0804930-mRNA-1:exon:8550;Parent=Msa0804930-mRNA-1 |
| chr5_3 | exon | 21310506 | 21310562 | 21310506 | ID=Msa0804930-mRNA-1:exon:8549;Parent=Msa0804930-mRNA-1 |
| chr5_3 | exon | 21310688 | 21310761 | 21310688 | ID=Msa0804930-mRNA-1:exon:8548;Parent=Msa0804930-mRNA-1 |
| chr5_3 | exon | 21311047 | 21311257 | 21311047 | ID=Msa0804930-mRNA-1:exon:8547;Parent=Msa0804930-mRNA-1 |
| chr5_3 | exon | 21311438 | 21311540 | 21311438 | ID=Msa0804930-mRNA-1:exon:8546;Parent=Msa0804930-mRNA-1 |
| chr5_3 | exon | 21311626 | 21311696 | 21311626 | ID=Msa0804930-mRNA-1:exon:8545;Parent=Msa0804930-mRNA-1 |
| chr5_3 | exon | 21311787 | 21311841 | 21311787 | ID=Msa0804930-mRNA-1:exon:8544;Parent=Msa0804930-mRNA-1 |
| chr5_3 | exon | 21312070 | 21312173 | 21312070 | ID=Msa0804930-mRNA-1:exon:8543;Parent=Msa0804930-mRNA-1 |
| chr5_3 | exon | 21312258 | 21312380 | 21312258 | ID=Msa0804930-mRNA-1:exon:8542;Parent=Msa0804930-mRNA-1 |
| chr5_3 | exon | 21312482 | 21312526 | 21312482 | ID=Msa0804930-mRNA-1:exon:8541;Parent=Msa0804930-mRNA-1 |
| chr5_3 | exon | 21314247 | 21314330 | 21314247 | ID=Msa0804930-mRNA-1:exon:8540;Parent=Msa0804930-mRNA-1 |
| chr5_3 | exon | 21314425 | 21314996 | 21314425 | ID=Msa0804930-mRNA-1:exon:8539;Parent=Msa0804930-mRNA-1 |
| chr5_3 | exon | 21317495 | 21317525 | 21317495 | ID=Msa0804930-mRNA-1:exon:8538;Parent=Msa0804930-mRNA-1 |
| chr5_3 | exon | 21317724 | 21317792 | 21317724 | ID=Msa0804930-mRNA-1:exon:8537;Parent=Msa0804930-mRNA-1 |
| chr5_3 | CDS | 21317724 | 21317792 | 21317724 | ID=Msa0804930-mRNA-1:cds;Parent=Msa0804930-mRNA-1 |
| chr5_3 | CDS | 21317495 | 21317525 | 21317495 | ID=Msa0804930-mRNA-1:cds;Parent=Msa0804930-mRNA-1 |
| chr5_3 | CDS | 21314425 | 21314996 | 21314425 | ID=Msa0804930-mRNA-1:cds;Parent=Msa0804930-mRNA-1 |
| chr5_3 | CDS | 21314247 | 21314330 | 21314247 | ID=Msa0804930-mRNA-1:cds;Parent=Msa0804930-mRNA-1 |
| chr5_3 | CDS | 21312482 | 21312526 | 21312482 | ID=Msa0804930-mRNA-1:cds;Parent=Msa0804930-mRNA-1 |
| chr5_3 | CDS | 21312258 | 21312380 | 21312258 | ID=Msa0804930-mRNA-1:cds;Parent=Msa0804930-mRNA-1 |
| chr5_3 | CDS | 21312070 | 21312173 | 21312070 | ID=Msa0804930-mRNA-1:cds;Parent=Msa0804930-mRNA-1 |
| chr5_3 | CDS | 21311787 | 21311841 | 21311787 | ID=Msa0804930-mRNA-1:cds;Parent=Msa0804930-mRNA-1 |
| chr5_3 | CDS | 21311626 | 21311696 | 21311626 | ID=Msa0804930-mRNA-1:cds;Parent=Msa0804930-mRNA-1 |
| chr5_3 | CDS | 21311438 | 21311540 | 21311438 | ID=Msa0804930-mRNA-1:cds;Parent=Msa0804930-mRNA-1 |
| chr5_3 | CDS | 21311047 | 21311257 | 21311047 | ID=Msa0804930-mRNA-1:cds;Parent=Msa0804930-mRNA-1 |
| chr5_3 | CDS | 21310688 | 21310761 | 21310688 | ID=Msa0804930-mRNA-1:cds;Parent=Msa0804930-mRNA-1 |
| chr5_3 | CDS | 21310506 | 21310562 | 21310506 | ID=Msa0804930-mRNA-1:cds;Parent=Msa0804930-mRNA-1 |
| chr5_3 | CDS | 21310342 | 21310416 | 21310342 | ID=Msa0804930-mRNA-1:cds;Parent=Msa0804930-mRNA-1 |
| chr5_3 | CDS | 21309558 | 21309617 | 21309558 | ID=Msa0804930-mRNA-1:cds;Parent=Msa0804930-mRNA-1 |
| chr5_3 | CDS | 21309229 | 21309303 | 21309229 | ID=Msa0804930-mRNA-1:cds;Parent=Msa0804930-mRNA-1 |
| chr5_3 | CDS | 21308869 | 21309150 | 21308869 | ID=Msa0804930-mRNA-1:cds;Parent=Msa0804930-mRNA-1 |
| chr5_3 | CDS | 21308516 | 21308580 | 21308516 | ID=Msa0804930-mRNA-1:cds;Parent=Msa0804930-mRNA-1 |
| chr5_3 | CDS | 21308377 | 21308437 | 21308377 | ID=Msa0804930-mRNA-1:cds;Parent=Msa0804930-mRNA-1 |
| chr5_3 | CDS | 21307909 | 21307989 | 21307909 | ID=Msa0804930-mRNA-1:cds;Parent=Msa0804930-mRNA-1 |
| chr5_3 | three_prime_UTR | 21307384 | 21307908 | 21307384 | ID=Msa0804930-mRNA-1:three_prime_utr;Parent=Msa0804930-mRNA-1 |
| Gene Sequence |
>Msa0804930 ATGGAGGTGAGGGTGTTGATGGGAAAATGGATTGTATTTGGGCTTTTTGTGCGTCTGAAAACCAAATGGGAGGATTTTGAAAAGTACGGAAAGAGAAAAGGAGAAAGAACAACTTCCATTGCCGCTTCTAATACACATGAACCACATAACACTCTTCTCAAACTTCTACCTTTCATCTCAAAAACCCATCATCATCTTCTTCCTCGTTACTTCGCTACAACCACCATGCCTCCACCACTCACCGGCACACATCACTATCATTACTTACTTCCTCCTTCAACCTCTCACCACCGTCATCACCACCGCCACCGCCACCAACCTCTAATCACATTCTCCTCCGTCAACCGCCACAATTTCACCCTCTCTTCTTCCAAACGCATTCTCTTTTCCTCTTCAATAAAATCAGCTTCCATCAATGAAGTTTCAATCCACAACAATTCAGAATCGACTTCCAATGCTTCTAACGAGTTTCTTGATAGAATACGAAAATGGGTTTCTTTCCTTCCTTCCATTTTCCCTGGTGGAACATGGTGGAACTTCTCCGAAGACGTTGAGGTTTCCATGTTCAATCAACCCGTTACCGTTTGGTATGCTTTGGGTAAAATGTGGAACCTTGTGGCGAAAGATAGATGGGTTATTTTCGCTGCTTTTTCTGCTCTTATCATTGCAGCAGTTTCGGAGATTTCTATACCACATTTTTTGACAGCATCGATATTCTCGGCGCAAAGTGGTGATATTAAAGTGTTTCATGGGAATGTTAAGAGAATGAGAGAAACATTATACTCTTCCCTTCTTCTTCAGGATATATCATTTTTTGACAATGAAACAGTAGGTGATTTGACAAGTAGACTTGGGGCAGATTGTCAGCAAGTTTCAAGGGTTATTGGAAATGATCTTAATTTGATACTGCGCAATGTCCTTCAGGGAGGAGGTTCATTGATCTACTTGTTGATTTTGTCTTGGCCACTTGGTTTATGTACGCTGGTGATATGCTCCATATTAGCAGCGGTCATGTTACGTTATGGATGGTACCAGAAGAAGGCAGCAAGGTTGATCCAAGAAGTCACTGCTTCGGCGAATAATGTAGCTCAAGAGACATTCTCTCTTATTAGAACTGTTCGAGTTTATGGAACCGAAGAAGAAGAACACGGAAGGTACAAATGGTGGCTTGAAAAATTAGCTGACATAAGCTTGCGACAAAGCGCTGCATATGGATTTTGGAATTTCAGCTTCAACACTCTTTATCATTCAACTCAGATTATTGCTGTGCTGTTTGGAGGAATGTCTATTCTATCGGGTCATATTACAGCAGAGAAACTTACTAAGTTTATACTATATAGTGAATGGTTAATTTATTCAACCTGGTGGGTGGGGGATAATGTATCCAATTTAATGCAATCAGTTGGAGCTAGTGAAAAGGTGTTCAACTTGATGGATCTCTCACCTAGCAGCCAATTTATAACAGAAGGAATGAAGTTGCAGAGTTTAACGGGGCATATTGAGTTTGTAAATGTATCTTTTCACTATCCTTCAAGACCAACGGTACATGTAGTGCAACATGTAAACTTTGTTGTCAATCCTAGTGAGGTGGTTGCCATTGTTGGTCTTAGTGGTAGCGGGAAAAGCACTTTGGTGAATCTATTACTCCGTCTCTACGAGCCTACAAGCGGTCAGGTTGAGAACTTCATGTGTTCTGAATCCAGGTGCTTATACCTTATTCTAGATTCTTTACTGATTTTGATAGATGGTGTCCCTCACAAAGACTTGGATGTCATGTGGTGGAGAGAGAGGATCGGATATGTGGGACAGGAACCAAAGTTATTCCGGATGGATATTAGCTCGAACATCAGATATGGATGTACCCGCGATGTAAATCAGGAGGATGTTGTGTGGGCTGCAAAGCAGGCCTATGCTCATGACTTTATCTCAGCACTTCCTAGTGGTTATGAAACACTTGTTGATGACGATCTCTTAAGTGGTGGACAAAAGCAACGAATTGCTATCGCTAGGGCCATTCTTAGGGACCCGAAAATATTGATTCTTGATGAAGCCACTAGTGCACTAGATGCTGAAAGTGAACATAATGTGAAGGGTGTTCTTCGATCGGTTAGAAGTGATTCTTCATCAAGGAGAAGTGTCATAGTCATTGCACACAGGCTTTCTACCATACAAGCTGCTGACAGGATTATTGTGATGGATAAGGGTCAAATAGTTGAGAATGGAAGTCACAGGGAACTTCTCTTGAAAGATGGTTTATATGCTCGATTGACTAGAAAACAGGCTGATACCATGGAATGA |
| Protein sequence |
>Msa0804930 MEVRVLMGKWIVFGLFVRLKTKWEDFEKYGKRKGERTTSIAASNTHEPHNTLLKLLPFISKTHHHLLPRYFATTTMPPPLTGTHHYHYLLPPSTSHHRHHHRHRHQPLITFSSVNRHNFTLSSSKRILFSSSIKSASINEVSIHNNSESTSNASNEFLDRIRKWVSFLPSIFPGGTWWNFSEDVEVSMFNQPVTVWYALGKMWNLVAKDRWVIFAAFSALIIAAVSEISIPHFLTASIFSAQSGDIKVFHGNVKRMRETLYSSLLLQDISFFDNETVGDLTSRLGADCQQVSRVIGNDLNLILRNVLQGGGSLIYLLILSWPLGLCTLVICSILAAVMLRYGWYQKKAARLIQEVTASANNVAQETFSLIRTVRVYGTEEEEHGRYKWWLEKLADISLRQSAAYGFWNFSFNTLYHSTQIIAVLFGGMSILSGHITAEKLTKFILYSEWLIYSTWWVGDNVSNLMQSVGASEKVFNLMDLSPSSQFITEGMKLQSLTGHIEFVNVSFHYPSRPTVHVVQHVNFVVNPSEVVAIVGLSGSGKSTLVNLLLRLYEPTSGQVENFMCSESRCLYLILDSLLILIDGVPHKDLDVMWWRERIGYVGQEPKLFRMDISSNIRYGCTRDVNQEDVVWAAKQAYAHDFISALPSGYETLVDDDLLSGGQKQRIAIARAILRDPKILILDEATSALDAESEHNVKGVLRSVRSDSSSRRSVIVIAHRLSTIQAADRIIVMDKGQIVENGSHRELLLKDGLYARLTRKQADTME* |
