Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1354330 | RHN59616.1 | 97.546 | 163 | 4 | 0 | 1 | 163 | 1 | 163 | 6.37e-111 | 322 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1354330 | sp|Q9BBT5|RR4_LOTJA | 95.513 | 156 | 7 | 0 | 1 | 156 | 1 | 156 | 7.07e-106 | 303 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1354330 | A0A396I1X4 | 97.546 | 163 | 4 | 0 | 1 | 163 | 1 | 163 | 3.04e-111 | 322 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0030710 | Msa1354330 | 0.806269 | 8.929463e-50 | -8.615850e-47 |
| Msa0095190 | Msa1354330 | 0.829275 | 5.637986e-55 | -8.615850e-47 |
| Msa1354330 | Msa1354350 | 0.872239 | 3.705292e-67 | -8.615850e-47 |
| Msa1354330 | Msa1354380 | 0.834550 | 2.808850e-56 | -8.615850e-47 |
| Msa1354330 | Msa1354390 | 0.862035 | 6.739668e-64 | -8.615850e-47 |
| Msa1354330 | Msa1354900 | 0.818682 | 1.725354e-52 | -8.615850e-47 |
| Msa1354330 | Msa1354910 | 0.822640 | 2.126259e-53 | -8.615850e-47 |
| Msa1354330 | Msa1354930 | 0.821086 | 4.868372e-53 | -8.615850e-47 |
| Msa1354330 | Msa1354950 | 0.829194 | 5.900948e-55 | -8.615850e-47 |
| Msa1354330 | Msa1355070 | 0.811625 | 6.375026e-51 | -8.615850e-47 |
| Msa1354330 | Msa1355080 | 0.816924 | 4.302942e-52 | -8.615850e-47 |
| Msa1354330 | Msa1355100 | 0.817546 | 3.118715e-52 | -8.615850e-47 |
| Msa1354330 | Msa1366000 | 0.820963 | 5.196476e-53 | -8.615850e-47 |
| Msa1354330 | Msa1366010 | 0.850936 | 1.230158e-60 | -8.615850e-47 |
| Msa1354330 | Msa1366040 | 0.807586 | 4.700982e-50 | -8.615850e-47 |
| Msa1354330 | Msa1366050 | 0.846763 | 1.770837e-59 | -8.615850e-47 |
| Msa1354330 | Msa1368270 | 0.849845 | 2.489549e-60 | -8.615850e-47 |
| Msa1354330 | Msa1368280 | 0.835541 | 1.579942e-56 | -8.615850e-47 |
| Msa1354330 | Msa1368290 | 0.837224 | 5.897566e-57 | -8.615850e-47 |
| Msa1354330 | Msa1368300 | 0.808722 | 2.693174e-50 | -8.615850e-47 |
| Msa1354330 | Msa1380590 | 0.817327 | 3.492353e-52 | -8.615850e-47 |
| Msa1354330 | Msa1380600 | 0.818372 | 2.029136e-52 | -8.615850e-47 |
| Msa1354330 | Msa1380620 | 0.822241 | 2.631986e-53 | -8.615850e-47 |
| Msa1354330 | Msa1380640 | 0.825332 | 4.967479e-54 | -8.615850e-47 |
| Msa1354330 | Msa1394040 | 0.814337 | 1.621919e-51 | -8.615850e-47 |
| Msa1354330 | Msa1394150 | 0.812174 | 4.841906e-51 | -8.615850e-47 |
| Msa1354330 | Msa1394170 | 0.807008 | 6.235346e-50 | -8.615850e-47 |
| Msa1354330 | Msa1394220 | 0.818172 | 2.251304e-52 | -8.615850e-47 |
| Msa1354330 | Msa1394230 | 0.817388 | 3.383468e-52 | -8.615850e-47 |
| Msa1354330 | Msa1398700 | 0.813112 | 3.017966e-51 | -8.615850e-47 |
| Msa1354330 | Msa1398720 | 0.809181 | 2.148360e-50 | -8.615850e-47 |
| Msa0704750 | Msa1354330 | 0.812501 | 4.107434e-51 | -8.615850e-47 |
| Msa0727020 | Msa1354330 | 0.821408 | 4.102025e-53 | -8.615850e-47 |
| Msa0186750 | Msa1354330 | 0.818691 | 1.717282e-52 | -8.615850e-47 |
| Msa1269400 | Msa1354330 | 0.828315 | 9.626011e-55 | -8.615850e-47 |
| Msa1269420 | Msa1354330 | 0.825448 | 4.661465e-54 | -8.615850e-47 |
| Msa0983720 | Msa1354330 | 0.812068 | 5.104836e-51 | -8.615850e-47 |
| Msa1086070 | Msa1354330 | 0.814641 | 1.389244e-51 | -8.615850e-47 |
| Msa1086080 | Msa1354330 | 0.811562 | 6.581120e-51 | -8.615850e-47 |
| Msa1086100 | Msa1354330 | 0.820574 | 6.382120e-53 | -8.615850e-47 |
| Msa1086140 | Msa1354330 | 0.808078 | 3.696083e-50 | -8.615850e-47 |
| Msa1086150 | Msa1354330 | 0.813877 | 2.048517e-51 | -8.615850e-47 |
| Msa1086180 | Msa1354330 | 0.823188 | 1.584580e-53 | -8.615850e-47 |
| Msa1086190 | Msa1354330 | 0.810198 | 1.298783e-50 | -8.615850e-47 |
| Msa1086210 | Msa1354330 | 0.825359 | 4.892430e-54 | -8.615850e-47 |
| Msa1086260 | Msa1354330 | 0.826039 | 3.375953e-54 | -8.615850e-47 |
| Msa1086280 | Msa1354330 | 0.810081 | 1.376159e-50 | -8.615850e-47 |
| Msa1086380 | Msa1354330 | 0.815750 | 7.876863e-52 | -8.615850e-47 |
| Msa1086450 | Msa1354330 | 0.815882 | 7.361926e-52 | -8.615850e-47 |
| Msa1086470 | Msa1354330 | 0.809465 | 1.867123e-50 | -8.615850e-47 |
| Msa1086880 | Msa1354330 | 0.817062 | 4.005701e-52 | -8.615850e-47 |
| Msa1086890 | Msa1354330 | 0.817469 | 3.245125e-52 | -8.615850e-47 |
| Msa1086910 | Msa1354330 | 0.826287 | 2.946593e-54 | -8.615850e-47 |
| Msa0268170 | Msa1354330 | 0.816514 | 5.317130e-52 | -8.615850e-47 |
| Msa0268390 | Msa1354330 | 0.809253 | 2.073100e-50 | -8.615850e-47 |
| Msa0268400 | Msa1354330 | 0.819165 | 1.340036e-52 | -8.615850e-47 |
| Msa0268410 | Msa1354330 | 0.818848 | 1.581925e-52 | -8.615850e-47 |
| Msa0268550 | Msa1354330 | 0.825394 | 4.801325e-54 | -8.615850e-47 |
| Msa0268560 | Msa1354330 | 0.827908 | 1.206307e-54 | -8.615850e-47 |
| Msa0268580 | Msa1354330 | 0.832594 | 8.645615e-56 | -8.615850e-47 |
| Msa0268630 | Msa1354330 | 0.811009 | 8.673266e-51 | -8.615850e-47 |
| Msa0268640 | Msa1354330 | 0.821161 | 4.676768e-53 | -8.615850e-47 |
| Msa0268720 | Msa1354330 | 0.815170 | 1.060363e-51 | -8.615850e-47 |
| Msa0268740 | Msa1354330 | 0.815526 | 8.838617e-52 | -8.615850e-47 |
| Msa0268760 | Msa1354330 | 0.825722 | 4.013658e-54 | -8.615850e-47 |
| Msa0268840 | Msa1354330 | 0.803227 | 3.855155e-49 | -8.615850e-47 |
| Msa0268940 | Msa1354330 | 0.802162 | 6.393244e-49 | -8.615850e-47 |
| Msa0268950 | Msa1354330 | 0.802178 | 6.347437e-49 | -8.615850e-47 |
| Msa0269040 | Msa1354330 | 0.805072 | 1.592824e-49 | -8.615850e-47 |
| Msa0269050 | Msa1354330 | 0.817803 | 2.728995e-52 | -8.615850e-47 |
| Msa0307490 | Msa1354330 | 0.805062 | 1.600248e-49 | -8.615850e-47 |
| Msa0344180 | Msa1354330 | 0.832472 | 9.271721e-56 | -8.615850e-47 |
| Msa0344190 | Msa1354330 | 0.823217 | 1.560360e-53 | -8.615850e-47 |
| Msa0344300 | Msa1354330 | 0.816427 | 5.561859e-52 | -8.615850e-47 |
| Msa0344310 | Msa1354330 | 0.821478 | 3.952897e-53 | -8.615850e-47 |
| Msa1143130 | Msa1354330 | 0.815176 | 1.057014e-51 | -8.615850e-47 |
| Msa1143160 | Msa1354330 | 0.814320 | 1.636340e-51 | -8.615850e-47 |
| Msa1143180 | Msa1354330 | 0.807725 | 4.392718e-50 | -8.615850e-47 |
| Msa1197210 | Msa1354330 | 0.802389 | 5.742023e-49 | -8.615850e-47 |
| Msa0577140 | Msa1354330 | 0.801366 | 9.314860e-49 | -8.615850e-47 |
| Msa0582510 | Msa1354330 | 0.800628 | 1.318343e-48 | -8.615850e-47 |
| Msa0582520 | Msa1354330 | 0.812416 | 4.286805e-51 | -8.615850e-47 |
| Msa0582630 | Msa1354330 | 0.810180 | 1.310117e-50 | -8.615850e-47 |
| Msa0582640 | Msa1354330 | 0.817154 | 3.819419e-52 | -8.615850e-47 |
| Msa0582660 | Msa1354330 | 0.815724 | 7.985319e-52 | -8.615850e-47 |
| Msa0582690 | Msa1354330 | 0.800229 | 1.589669e-48 | -8.615850e-47 |
| Msa0582700 | Msa1354330 | 0.820626 | 6.211538e-53 | -8.615850e-47 |
| Msa0582750 | Msa1354330 | 0.817653 | 2.949094e-52 | -8.615850e-47 |
| Msa0582760 | Msa1354330 | 0.812799 | 3.535278e-51 | -8.615850e-47 |
| Msa0582780 | Msa1354330 | 0.822597 | 2.175935e-53 | -8.615850e-47 |
| Msa0582820 | Msa1354330 | 0.823034 | 1.721409e-53 | -8.615850e-47 |
| Msa0582910 | Msa1354330 | 0.806965 | 6.366170e-50 | -8.615850e-47 |
| Msa0582930 | Msa1354330 | 0.823984 | 1.031879e-53 | -8.615850e-47 |
| Msa0591370 | Msa1354330 | 0.832871 | 7.381439e-56 | -8.615850e-47 |
| Msa0867640 | Msa1354330 | 0.807139 | 5.847496e-50 | -8.615850e-47 |
| Msa0883880 | Msa1354330 | 0.800257 | 1.569003e-48 | -8.615850e-47 |
| Msa0924870 | Msa1354330 | 0.820454 | 6.800858e-53 | -8.615850e-47 |
| Msa0924890 | Msa1354330 | 0.809300 | 2.025407e-50 | -8.615850e-47 |
| Msa0931070 | Msa1354330 | 0.803442 | 3.479087e-49 | -8.615850e-47 |
| Msa0931080 | Msa1354330 | 0.817729 | 2.835988e-52 | -8.615850e-47 |
| Msa0931100 | Msa1354330 | 0.805175 | 1.515544e-49 | -8.615850e-47 |
| Msa0931130 | Msa1354330 | 0.809206 | 2.122001e-50 | -8.615850e-47 |
| Msa0931140 | Msa1354330 | 0.814125 | 1.806311e-51 | -8.615850e-47 |
| Msa0931210 | Msa1354330 | 0.824748 | 6.821873e-54 | -8.615850e-47 |
| Msa0931220 | Msa1354330 | 0.802712 | 4.926168e-49 | -8.615850e-47 |
| Msa0931240 | Msa1354330 | 0.839904 | 1.198592e-57 | -8.615850e-47 |
| Msa0931270 | Msa1354330 | 0.810305 | 1.231363e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1354330 | MtrunA17_Chr4g0015291 | 97.546 | 163 | 4 | 0 | 1 | 163 | 1 | 163 | 5.86e-115 | 322 |
| Msa1354330 | MtrunA17_Chr7g0229301 | 97.436 | 156 | 4 | 0 | 1 | 156 | 1 | 156 | 2.14e-108 | 307 |
| Msa1354330 | MtrunA17_Chr6g0454441 | 97.436 | 156 | 4 | 0 | 1 | 156 | 1 | 156 | 2.14e-108 | 307 |
| Msa1354330 | MtrunA17_Chr4g0016451 | 97.436 | 156 | 4 | 0 | 1 | 156 | 1 | 156 | 2.14e-108 | 307 |
| Msa1354330 | MtrunA17_Chr6g0466281 | 97.436 | 156 | 4 | 0 | 1 | 156 | 1 | 156 | 2.14e-108 | 307 |
| Msa1354330 | MtrunA17_CPg0493311 | 97.436 | 156 | 4 | 0 | 1 | 156 | 1 | 156 | 2.14e-108 | 307 |
| Msa1354330 | MtrunA17_Chr7g0229661 | 82.051 | 156 | 9 | 1 | 1 | 156 | 1 | 137 | 9.59e-83 | 242 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1354330 | ATCG00380.1 | 89.677 | 155 | 16 | 0 | 1 | 155 | 1 | 155 | 1.76e-98 | 282 |
Find 25 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTAAATACGTTCGTATCGC+TGG | 0.342652 | tig0014543:-55357 | Msa1354330:CDS |
| ACAATTACTTGAAATGCGTT+TGG | 0.359489 | tig0014543:-55301 | Msa1354330:CDS |
| TACCAGGACTAACTAGTAAA+CGG | 0.463029 | tig0014543:-55500 | Msa1354330:CDS |
| TGAGGTTTGCAACGATAACT+TGG | 0.468099 | tig0014543:+55177 | None:intergenic |
| GTCTGTGGTTAACTAATTGA+CGG | 0.477609 | tig0014543:+55232 | None:intergenic |
| ATGGATTGGCAATTCTTCGC+GGG | 0.483141 | tig0014543:+55087 | None:intergenic |
| CTAAGTTCGCTTCCAACTGT+AGG | 0.508663 | tig0014543:+55477 | None:intergenic |
| GGATCAAGAGTCAAATGGAT+TGG | 0.511159 | tig0014543:+55073 | None:intergenic |
| GGAAAAGCGAAAGGTTCAAC+CGG | 0.516182 | tig0014543:-55336 | Msa1354330:CDS |
| TCTGTGGTTAACTAATTGAC+GGG | 0.521261 | tig0014543:+55233 | None:intergenic |
| GGCCGTTTACTAGTTAGTCC+TGG | 0.527000 | tig0014543:+55498 | None:intergenic |
| AATGGATTGGCAATTCTTCG+CGG | 0.545268 | tig0014543:+55086 | None:intergenic |
| TCAAGATATTATTACAGCGA+AGG | 0.559737 | tig0014543:-55157 | Msa1354330:CDS |
| TTCGCTGTAATAATATCTTG+AGG | 0.560954 | tig0014543:+55159 | None:intergenic |
| TAATTGTAGTAAAACTTGAC+CGG | 0.568250 | tig0014543:+55317 | None:intergenic |
| ACTAGTAAACGGCCTACAGT+TGG | 0.568372 | tig0014543:-55489 | Msa1354330:CDS |
| CAAGATATTATTACAGCGAA+GGG | 0.569002 | tig0014543:-55156 | Msa1354330:CDS |
| TTAACTAATTGACGGGCTTG+AGG | 0.581554 | tig0014543:+55240 | None:intergenic |
| GGAATGGATCAAGAGTCAAA+TGG | 0.582974 | tig0014543:+55068 | None:intergenic |
| TGGATTGGCAATTCTTCGCG+GGG | 0.587059 | tig0014543:+55088 | None:intergenic |
| TGTTTATGTCACGTTACAGA+GGG | 0.632465 | tig0014543:-55557 | None:intergenic |
| GTGTTTATGTCACGTTACAG+AGG | 0.633128 | tig0014543:-55558 | None:intergenic |
| CGTATCGCTGGAAAAGCGAA+AGG | 0.633501 | tig0014543:-55345 | Msa1354330:CDS |
| CATTAACTAAAACATGTCTG+TGG | 0.663264 | tig0014543:+55217 | None:intergenic |
| GGATTGGCAATTCTTCGCGG+GGG | 0.725981 | tig0014543:+55089 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CAAAAATTGCGTTTTCATTA+TGG | - | tig0014543:55236-55255 | Msa1354330:CDS | 25.0% |
| ! | TAATTGTAGTAAAACTTGAC+CGG | + | tig0014543:55321-55340 | None:intergenic | 25.0% |
| !!! | GATAACATACTTTTTCGATT+GGG | - | tig0014543:55356-55375 | Msa1354330:CDS | 25.0% |
| TTTCAAAAAAATACGTCGTC+TGG | - | tig0014543:55106-55125 | Msa1354330:CDS | 30.0% | |
| TTCAAAAAAATACGTCGTCT+GGG | - | tig0014543:55107-55126 | Msa1354330:CDS | 30.0% | |
| ACAATTACTTGAAATGCGTT+TGG | - | tig0014543:55334-55353 | Msa1354330:CDS | 30.0% | |
| !!! | GGATAACATACTTTTTCGAT+TGG | - | tig0014543:55355-55374 | Msa1354330:CDS | 30.0% |
| CATTAACTAAAACATGTCTG+TGG | + | tig0014543:55421-55440 | None:intergenic | 30.0% | |
| !!! | CACAGACATGTTTTAGTTAA+TGG | - | tig0014543:55419-55438 | Msa1354330:CDS | 30.0% |
| TTCGCTGTAATAATATCTTG+AGG | + | tig0014543:55479-55498 | None:intergenic | 30.0% | |
| TCAAGATATTATTACAGCGA+AGG | - | tig0014543:55478-55497 | Msa1354330:CDS | 30.0% | |
| CAAGATATTATTACAGCGAA+GGG | - | tig0014543:55479-55498 | Msa1354330:CDS | 30.0% | |
| !!! | CGACGTATTTTTTTGAAACG+AGG | + | tig0014543:55104-55123 | None:intergenic | 35.0% |
| TCAAAAAAATACGTCGTCTG+GGG | - | tig0014543:55108-55127 | Msa1354330:CDS | 35.0% | |
| TACCAGGACTAACTAGTAAA+CGG | - | tig0014543:55135-55154 | Msa1354330:CDS | 35.0% | |
| !!! | CATACTTTTTCGATTGGGTA+TGG | - | tig0014543:55361-55380 | Msa1354330:CDS | 35.0% |
| TCTGTGGTTAACTAATTGAC+GGG | + | tig0014543:55405-55424 | None:intergenic | 35.0% | |
| GTCTGTGGTTAACTAATTGA+CGG | + | tig0014543:55406-55425 | None:intergenic | 35.0% | |
| CAAAAAAATACGTCGTCTGG+GGG | - | tig0014543:55109-55128 | Msa1354330:CDS | 40.0% | |
| !!! | ATTTTTTACTCGAGCGCGAT+TGG | + | tig0014543:55187-55206 | None:intergenic | 40.0% |
| CTTAAATACGTTCGTATCGC+TGG | - | tig0014543:55278-55297 | Msa1354330:CDS | 40.0% | |
| !! | TTAACTAATTGACGGGCTTG+AGG | + | tig0014543:55398-55417 | None:intergenic | 40.0% |
| TGAGGTTTGCAACGATAACT+TGG | + | tig0014543:55461-55480 | None:intergenic | 40.0% | |
| !! | AATGGATTGGCAATTCTTCG+CGG | + | tig0014543:55552-55571 | None:intergenic | 40.0% |
| ACTAGTAAACGGCCTACAGT+TGG | - | tig0014543:55146-55165 | Msa1354330:CDS | 45.0% | |
| CTAAGTTCGCTTCCAACTGT+AGG | + | tig0014543:55161-55180 | None:intergenic | 45.0% | |
| GGAAAAGCGAAAGGTTCAAC+CGG | - | tig0014543:55299-55318 | Msa1354330:CDS | 45.0% | |
| !! | ATGGATTGGCAATTCTTCGC+GGG | + | tig0014543:55551-55570 | None:intergenic | 45.0% |
| GGCCGTTTACTAGTTAGTCC+TGG | + | tig0014543:55140-55159 | None:intergenic | 50.0% | |
| CGTATCGCTGGAAAAGCGAA+AGG | - | tig0014543:55290-55309 | Msa1354330:CDS | 50.0% | |
| !! | TGGATTGGCAATTCTTCGCG+GGG | + | tig0014543:55550-55569 | None:intergenic | 50.0% |
| ! | GGATTGGCAATTCTTCGCGG+GGG | + | tig0014543:55549-55568 | None:intergenic | 55.0% |
| CGTCGTCTGGGGGCTTTACC+AGG | - | tig0014543:55119-55138 | Msa1354330:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0014543 | gene | 55083 | 55574 | 55083 | ID=Msa1354330;Name=Msa1354330 |
| tig0014543 | mRNA | 55083 | 55574 | 55083 | ID=Msa1354330-mRNA-1;Parent=Msa1354330;Name=Msa1354330-mRNA-1;_AED=0.09;_eAED=0.12;_QI=0|-1|0|1|-1|1|1|0|163 |
| tig0014543 | exon | 55083 | 55574 | 55083 | ID=Msa1354330-mRNA-1:exon:9088;Parent=Msa1354330-mRNA-1 |
| tig0014543 | CDS | 55083 | 55574 | 55083 | ID=Msa1354330-mRNA-1:cds;Parent=Msa1354330-mRNA-1 |
| Gene Sequence |
| Protein sequence |
