Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1000180 | KAE8705844.1 | 76.190 | 147 | 35 | 0 | 4 | 150 | 16 | 162 | 3.63e-70 | 220 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1000180 | sp|Q9SZD6|PETS_ARATH | 71.429 | 147 | 42 | 0 | 4 | 150 | 788 | 934 | 5.89e-61 | 205 |
| Msa1000180 | sp|Q9SZD6|PETS_ARATH | 63.699 | 146 | 53 | 0 | 5 | 150 | 551 | 696 | 3.35e-53 | 184 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1000180 | A0A6A3AMK8 | 76.190 | 147 | 35 | 0 | 4 | 150 | 16 | 162 | 1.73e-70 | 220 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0006070 | Msa1000180 | 0.818780 | 1.639533e-52 | -8.615850e-47 |
| Msa0006110 | Msa1000180 | 0.822078 | 2.870778e-53 | -8.615850e-47 |
| Msa0014710 | Msa1000180 | 0.823806 | 1.136152e-53 | -8.615850e-47 |
| Msa0017510 | Msa1000180 | 0.807566 | 4.747956e-50 | -8.615850e-47 |
| Msa0072900 | Msa1000180 | 0.804646 | 1.954805e-49 | -8.615850e-47 |
| Msa0075470 | Msa1000180 | 0.814742 | 1.319243e-51 | -8.615850e-47 |
| Msa0084380 | Msa1000180 | 0.809168 | 2.162257e-50 | -8.615850e-47 |
| Msa0091210 | Msa1000180 | -0.804221 | 2.396887e-49 | -8.615850e-47 |
| Msa0097810 | Msa1000180 | 0.803785 | 2.953863e-49 | -8.615850e-47 |
| Msa0105070 | Msa1000180 | 0.812647 | 3.816888e-51 | -8.615850e-47 |
| Msa0107640 | Msa1000180 | 0.814090 | 1.838545e-51 | -8.615850e-47 |
| Msa0114060 | Msa1000180 | 0.816786 | 4.619909e-52 | -8.615850e-47 |
| Msa0613440 | Msa1000180 | 0.803770 | 2.974443e-49 | -8.615850e-47 |
| Msa0625720 | Msa1000180 | 0.809654 | 1.700091e-50 | -8.615850e-47 |
| Msa0661650 | Msa1000180 | 0.836805 | 7.543169e-57 | -8.615850e-47 |
| Msa0663390 | Msa1000180 | 0.810827 | 9.496366e-51 | -8.615850e-47 |
| Msa0663810 | Msa1000180 | 0.801069 | 1.071169e-48 | -8.615850e-47 |
| Msa0669320 | Msa1000180 | 0.815562 | 8.674759e-52 | -8.615850e-47 |
| Msa0679290 | Msa1000180 | -0.817881 | 2.620118e-52 | -8.615850e-47 |
| Msa0681250 | Msa1000180 | 0.801107 | 1.052623e-48 | -8.615850e-47 |
| Msa0681270 | Msa1000180 | 0.806931 | 6.473212e-50 | -8.615850e-47 |
| Msa0683510 | Msa1000180 | 0.812385 | 4.353653e-51 | -8.615850e-47 |
| Msa0691380 | Msa1000180 | 0.804584 | 2.014065e-49 | -8.615850e-47 |
| Msa0712590 | Msa1000180 | 0.803379 | 3.585701e-49 | -8.615850e-47 |
| Msa0724210 | Msa1000180 | 0.824918 | 6.219870e-54 | -8.615850e-47 |
| Msa0731940 | Msa1000180 | 0.818054 | 2.393884e-52 | -8.615850e-47 |
| Msa0760130 | Msa1000180 | 0.803064 | 4.165956e-49 | -8.615850e-47 |
| Msa0762970 | Msa1000180 | 0.821590 | 3.724029e-53 | -8.615850e-47 |
| Msa0766630 | Msa1000180 | 0.800975 | 1.119817e-48 | -8.615850e-47 |
| Msa0790510 | Msa1000180 | 0.806095 | 9.715495e-50 | -8.615850e-47 |
| Msa0816710 | Msa1000180 | 0.810697 | 1.013236e-50 | -8.615850e-47 |
| Msa0832340 | Msa1000180 | 0.806653 | 7.409545e-50 | -8.615850e-47 |
| Msa0840820 | Msa1000180 | 0.805458 | 1.322287e-49 | -8.615850e-47 |
| Msa0121090 | Msa1000180 | 0.802627 | 5.129453e-49 | -8.615850e-47 |
| Msa0140500 | Msa1000180 | -0.803641 | 3.163618e-49 | -8.615850e-47 |
| Msa0157290 | Msa1000180 | 0.807118 | 5.909285e-50 | -8.615850e-47 |
| Msa0212990 | Msa1000180 | 0.805544 | 1.267982e-49 | -8.615850e-47 |
| Msa0218810 | Msa1000180 | 0.801117 | 1.047645e-48 | -8.615850e-47 |
| Msa0223690 | Msa1000180 | 0.802181 | 6.335971e-49 | -8.615850e-47 |
| Msa0367870 | Msa1000180 | 0.820079 | 8.288888e-53 | -8.615850e-47 |
| Msa0410650 | Msa1000180 | 0.808214 | 3.456829e-50 | -8.615850e-47 |
| Msa0417270 | Msa1000180 | 0.809989 | 1.440617e-50 | -8.615850e-47 |
| Msa0440400 | Msa1000180 | 0.806315 | 8.732849e-50 | -8.615850e-47 |
| Msa0440750 | Msa1000180 | 0.817903 | 2.590490e-52 | -8.615850e-47 |
| Msa0464680 | Msa1000180 | 0.816815 | 4.552883e-52 | -8.615850e-47 |
| Msa1000170 | Msa1000180 | 0.812045 | 5.164550e-51 | -8.615850e-47 |
| Msa1000180 | Msa1000190 | 0.809546 | 1.793661e-50 | -8.615850e-47 |
| Msa1000180 | Msa1000370 | 0.901097 | 3.780861e-78 | -8.615850e-47 |
| Msa1000180 | Msa1000860 | 0.802387 | 5.747591e-49 | -8.615850e-47 |
| Msa1000180 | Msa1000890 | 0.810982 | 8.790080e-51 | -8.615850e-47 |
| Msa1000180 | Msa1014540 | 0.825187 | 5.373032e-54 | -8.615850e-47 |
| Msa1000180 | Msa1016320 | 0.818876 | 1.559539e-52 | -8.615850e-47 |
| Msa1000180 | Msa1036610 | 0.803263 | 3.789433e-49 | -8.615850e-47 |
| Msa1000180 | Msa1075540 | 0.811112 | 8.239792e-51 | -8.615850e-47 |
| Msa1000180 | Msa1075650 | 0.806674 | 7.336207e-50 | -8.615850e-47 |
| Msa1000180 | Msa1082500 | 0.819884 | 9.188765e-53 | -8.615850e-47 |
| Msa1000180 | Msa1085200 | 0.810213 | 1.289245e-50 | -8.615850e-47 |
| Msa1000180 | Msa1103980 | 0.800313 | 1.528242e-48 | -8.615850e-47 |
| Msa1000180 | Msa1104090 | 0.814536 | 1.465421e-51 | -8.615850e-47 |
| Msa1000180 | Msa1111870 | 0.804720 | 1.886609e-49 | -8.615850e-47 |
| Msa1000180 | Msa1113170 | 0.832345 | 9.966693e-56 | -8.615850e-47 |
| Msa1000180 | Msa1121490 | 0.813424 | 2.578183e-51 | -8.615850e-47 |
| Msa1000180 | Msa1130550 | 0.806792 | 6.925959e-50 | -8.615850e-47 |
| Msa1000180 | Msa1162550 | -0.804055 | 2.595171e-49 | -8.615850e-47 |
| Msa1000180 | Msa1162780 | 0.827686 | 1.363734e-54 | -8.615850e-47 |
| Msa1000180 | Msa1282580 | -0.800163 | 1.639735e-48 | -8.615850e-47 |
| Msa1000180 | Msa1284680 | 0.814615 | 1.407689e-51 | -8.615850e-47 |
| Msa1000180 | Msa1309030 | 0.832479 | 9.232538e-56 | -8.615850e-47 |
| Msa1000180 | Msa1341520 | 0.819756 | 9.827517e-53 | -8.615850e-47 |
| Msa1000180 | Msa1353470 | 0.805804 | 1.118668e-49 | -8.615850e-47 |
| Msa1000180 | Msa1373030 | 0.808743 | 2.666235e-50 | -8.615850e-47 |
| Msa1000180 | Msa1392240 | 0.803119 | 4.058515e-49 | -8.615850e-47 |
| Msa1000180 | Msa1392260 | 0.804666 | 1.935975e-49 | -8.615850e-47 |
| Msa1000180 | Msa1407640 | -0.818394 | 2.005688e-52 | -8.615850e-47 |
| Msa1000180 | Msa1413610 | 0.811870 | 5.638517e-51 | -8.615850e-47 |
| Msa1000180 | Msa1415910 | 0.813771 | 2.162347e-51 | -8.615850e-47 |
| Msa1000180 | Msa1423880 | 0.808658 | 2.779831e-50 | -8.615850e-47 |
| Msa1000180 | Msa1445390 | -0.803531 | 3.335210e-49 | -8.615850e-47 |
| Msa1000180 | Msa1449340 | -0.800755 | 1.241628e-48 | -8.615850e-47 |
| Msa1000180 | Msa1457040 | 0.815335 | 9.746483e-52 | -8.615850e-47 |
| Msa1000180 | Msa1466540 | 0.809925 | 1.486736e-50 | -8.615850e-47 |
| Msa0243460 | Msa1000180 | 0.800629 | 1.317904e-48 | -8.615850e-47 |
| Msa0267370 | Msa1000180 | 0.830099 | 3.553718e-55 | -8.615850e-47 |
| Msa0285450 | Msa1000180 | 0.811969 | 5.365335e-51 | -8.615850e-47 |
| Msa0297790 | Msa1000180 | 0.805971 | 1.031492e-49 | -8.615850e-47 |
| Msa0309770 | Msa1000180 | 0.821069 | 4.911950e-53 | -8.615850e-47 |
| Msa0330340 | Msa1000180 | 0.821744 | 3.430987e-53 | -8.615850e-47 |
| Msa0333030 | Msa1000180 | 0.801730 | 7.843459e-49 | -8.615850e-47 |
| Msa0363680 | Msa1000180 | 0.808102 | 3.652879e-50 | -8.615850e-47 |
| Msa0363790 | Msa1000180 | 0.805620 | 1.222343e-49 | -8.615850e-47 |
| Msa0507680 | Msa1000180 | 0.804008 | 2.654975e-49 | -8.615850e-47 |
| Msa0529890 | Msa1000180 | 0.809216 | 2.111482e-50 | -8.615850e-47 |
| Msa0553240 | Msa1000180 | 0.811576 | 6.533983e-51 | -8.615850e-47 |
| Msa0569500 | Msa1000180 | 0.803335 | 3.661262e-49 | -8.615850e-47 |
| Msa0585560 | Msa1000180 | 0.809739 | 1.630152e-50 | -8.615850e-47 |
| Msa0843260 | Msa1000180 | 0.805425 | 1.343198e-49 | -8.615850e-47 |
| Msa0875290 | Msa1000180 | 0.803645 | 3.158119e-49 | -8.615850e-47 |
| Msa0880140 | Msa1000180 | 0.808440 | 3.094401e-50 | -8.615850e-47 |
| Msa0897330 | Msa1000180 | 0.832777 | 7.790047e-56 | -8.615850e-47 |
| Msa0926890 | Msa1000180 | 0.821220 | 4.532920e-53 | -8.615850e-47 |
| Msa0930830 | Msa1000180 | -0.817594 | 3.041064e-52 | -8.615850e-47 |
| Msa0960720 | Msa1000180 | 0.803258 | 3.798278e-49 | -8.615850e-47 |
| Msa0965610 | Msa1000180 | 0.808734 | 2.678081e-50 | -8.615850e-47 |
| Msa0969520 | Msa1000180 | 0.836609 | 8.464553e-57 | -8.615850e-47 |
| Msa0974070 | Msa1000180 | 0.819756 | 9.827078e-53 | -8.615850e-47 |
| Msa0974080 | Msa1000180 | 0.803271 | 3.775154e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1000180 | MtrunA17_Chr6g0486531 | 82.313 | 147 | 26 | 0 | 4 | 150 | 881 | 1027 | 2.01e-73 | 237 |
| Msa1000180 | MtrunA17_Chr6g0486531 | 60.959 | 146 | 57 | 0 | 5 | 150 | 641 | 786 | 2.80e-53 | 181 |
| Msa1000180 | MtrunA17_Chr2g0299581 | 64.667 | 150 | 35 | 3 | 2 | 150 | 32 | 164 | 3.80e-52 | 164 |
| Msa1000180 | MtrunA17_Chr6g0484381 | 61.074 | 149 | 29 | 1 | 2 | 150 | 34 | 153 | 1.80e-48 | 154 |
| Msa1000180 | MtrunA17_Chr6g0486321 | 60.000 | 145 | 28 | 2 | 5 | 149 | 72 | 186 | 2.49e-45 | 147 |
| Msa1000180 | MtrunA17_Chr6g0486461 | 78.481 | 79 | 17 | 0 | 72 | 150 | 10 | 88 | 1.37e-35 | 119 |
| Msa1000180 | MtrunA17_Chr6g0486491 | 63.830 | 94 | 20 | 1 | 4 | 97 | 15 | 94 | 7.00e-32 | 109 |
| Msa1000180 | MtrunA17_Chr6g0460441 | 43.220 | 118 | 46 | 1 | 5 | 122 | 45 | 141 | 5.98e-22 | 86.3 |
| Msa1000180 | MtrunA17_Chr6g0486241 | 42.466 | 146 | 29 | 3 | 5 | 150 | 76 | 166 | 1.69e-20 | 83.6 |
| Msa1000180 | MtrunA17_Chr6g0486281 | 82.979 | 47 | 8 | 0 | 68 | 114 | 1 | 47 | 5.99e-20 | 78.2 |
| Msa1000180 | MtrunA17_Chr6g0477831 | 51.250 | 80 | 39 | 0 | 16 | 95 | 11 | 90 | 8.89e-20 | 79.0 |
| Msa1000180 | MtrunA17_Chr6g0486301 | 48.810 | 84 | 33 | 1 | 31 | 114 | 1031 | 1104 | 6.11e-17 | 77.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1000180 | AT4G29060.1 | 71.429 | 147 | 42 | 0 | 4 | 150 | 788 | 934 | 5.99e-62 | 205 |
| Msa1000180 | AT4G29060.1 | 63.699 | 146 | 53 | 0 | 5 | 150 | 551 | 696 | 3.40e-54 | 184 |
| Msa1000180 | AT4G29060.2 | 64.384 | 146 | 46 | 1 | 5 | 150 | 551 | 690 | 5.86e-53 | 179 |
Find 25 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCTTCATCTGCTAATCTTC+TGG | 0.187100 | 6_4:+82899733 | None:intergenic |
| GGAAGAATCTCAAAGAAGTT+AGG | 0.377662 | 6_4:-82899452 | Msa1000180:CDS |
| TTAGCAGATGAAGGAAGAAA+AGG | 0.433566 | 6_4:-82899725 | Msa1000180:CDS |
| GGATTTCTTATCAGCGGATG+AGG | 0.463133 | 6_4:+82899754 | None:intergenic |
| AAAGCCCTTGCTGAAACATA+AGG | 0.491814 | 6_4:-82899804 | None:intergenic |
| GATAAGAGAGAAAATTGTTG+AGG | 0.494347 | 6_4:-82899474 | Msa1000180:CDS |
| ATAAGAGAGAAAATTGTTGA+GGG | 0.494474 | 6_4:-82899473 | Msa1000180:CDS |
| CTCACCTTATGTTTCAGCAA+GGG | 0.504959 | 6_4:+82899800 | None:intergenic |
| TCCAGAAGATTAGCAGATGA+AGG | 0.507836 | 6_4:-82899734 | Msa1000180:CDS |
| AAGTGAGAAATTCAAGGAGT+TGG | 0.507877 | 6_4:-82899633 | Msa1000180:CDS |
| TGGCAAAAGTGAGAAATTCA+AGG | 0.510573 | 6_4:-82899639 | Msa1000180:CDS |
| GCAAGTTGCTGCATGTCCAC+AGG | 0.512986 | 6_4:-82899594 | Msa1000180:CDS |
| TTGAACTATTTCTCCAAGAG+TGG | 0.526704 | 6_4:+82899323 | None:intergenic |
| AACTGTGAAACCGACTTTGT+TGG | 0.539216 | 6_4:-82899659 | Msa1000180:CDS |
| TCTCGTAACCATTGTGACAA+AGG | 0.559968 | 6_4:-82899546 | Msa1000180:CDS |
| TCTTCTGGATTTCTTATCAG+CGG | 0.572880 | 6_4:+82899748 | None:intergenic |
| GAACAAAACGCCACACTCAT+CGG | 0.575627 | 6_4:+82899349 | None:intergenic |
| GATTTCTTATCAGCGGATGA+GGG | 0.595961 | 6_4:+82899755 | None:intergenic |
| ACAGACACAAACTGAACCTG+TGG | 0.624395 | 6_4:+82899578 | None:intergenic |
| CAGTGTTTCAGTGAAAGACT+CGG | 0.628061 | 6_4:-82899390 | Msa1000180:CDS |
| AGTATAGCTGCCGATGAGTG+TGG | 0.629024 | 6_4:-82899359 | Msa1000180:CDS |
| GCTCACCTTATGTTTCAGCA+AGG | 0.653488 | 6_4:+82899799 | None:intergenic |
| AGTTCACTTCAATTAGAACA+CGG | 0.682971 | 6_4:+82899678 | None:intergenic |
| TTTCAGTGAAAGACTCGGTG+AGG | 0.683646 | 6_4:-82899385 | Msa1000180:CDS |
| ACACTGTGATCGTTATTGAA+AGG | 0.689287 | 6_4:+82899407 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTTTCTCTCTTATCTTCT+CGG | + | chr6_4:82899647-82899666 | None:intergenic | 25.0% |
| ! | ATAAGAGAGAAAATTGTTGA+GGG | - | chr6_4:82899652-82899671 | Msa1000180:CDS | 25.0% |
| AGTTCACTTCAATTAGAACA+CGG | + | chr6_4:82899450-82899469 | None:intergenic | 30.0% | |
| ! | TTCTTTTTCCTTTGTCACAA+TGG | + | chr6_4:82899590-82899609 | None:intergenic | 30.0% |
| ! | ATTTTCTCTCTTATCTTCTC+GGG | + | chr6_4:82899646-82899665 | None:intergenic | 30.0% |
| GATAAGAGAGAAAATTGTTG+AGG | - | chr6_4:82899651-82899670 | Msa1000180:CDS | 30.0% | |
| AGCGCATACATCAGAAAAAA+GGG | - | chr6_4:82899346-82899365 | Msa1000180:CDS | 35.0% | |
| TCTTCTGGATTTCTTATCAG+CGG | + | chr6_4:82899380-82899399 | None:intergenic | 35.0% | |
| TTAGCAGATGAAGGAAGAAA+AGG | - | chr6_4:82899400-82899419 | Msa1000180:CDS | 35.0% | |
| TGGCAAAAGTGAGAAATTCA+AGG | - | chr6_4:82899486-82899505 | Msa1000180:CDS | 35.0% | |
| AAGTGAGAAATTCAAGGAGT+TGG | - | chr6_4:82899492-82899511 | Msa1000180:CDS | 35.0% | |
| GGAAGAATCTCAAAGAAGTT+AGG | - | chr6_4:82899673-82899692 | Msa1000180:CDS | 35.0% | |
| ACACTGTGATCGTTATTGAA+AGG | + | chr6_4:82899721-82899740 | None:intergenic | 35.0% | |
| GAGCGCATACATCAGAAAAA+AGG | - | chr6_4:82899345-82899364 | Msa1000180:CDS | 40.0% | |
| GATTTCTTATCAGCGGATGA+GGG | + | chr6_4:82899373-82899392 | None:intergenic | 40.0% | |
| TCCAGAAGATTAGCAGATGA+AGG | - | chr6_4:82899391-82899410 | Msa1000180:CDS | 40.0% | |
| TCCTTCATCTGCTAATCTTC+TGG | + | chr6_4:82899395-82899414 | None:intergenic | 40.0% | |
| ! | AACTGTGAAACCGACTTTGT+TGG | - | chr6_4:82899466-82899485 | Msa1000180:CDS | 40.0% |
| !! | CTCACTTTTGCCAACAAAGT+CGG | + | chr6_4:82899479-82899498 | None:intergenic | 40.0% |
| TCTCGTAACCATTGTGACAA+AGG | - | chr6_4:82899579-82899598 | Msa1000180:CDS | 40.0% | |
| CAGTGTTTCAGTGAAAGACT+CGG | - | chr6_4:82899735-82899754 | Msa1000180:CDS | 40.0% | |
| GGATTTCTTATCAGCGGATG+AGG | + | chr6_4:82899374-82899393 | None:intergenic | 45.0% | |
| ACAGACACAAACTGAACCTG+TGG | + | chr6_4:82899550-82899569 | None:intergenic | 45.0% | |
| !! | TTTCAGTGAAAGACTCGGTG+AGG | - | chr6_4:82899740-82899759 | Msa1000180:CDS | 45.0% |
| GAACAAAACGCCACACTCAT+CGG | + | chr6_4:82899779-82899798 | None:intergenic | 45.0% | |
| !! | GATGAGTGTGGCGTTTTGTT+CGG | - | chr6_4:82899778-82899797 | Msa1000180:CDS | 45.0% |
| AGTATAGCTGCCGATGAGTG+TGG | - | chr6_4:82899766-82899785 | Msa1000180:CDS | 50.0% | |
| !! | CGTTTTGTTCGGTCCACTCT+TGG | - | chr6_4:82899789-82899808 | Msa1000180:CDS | 50.0% |
| GCAAGTTGCTGCATGTCCAC+AGG | - | chr6_4:82899531-82899550 | Msa1000180:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_4 | gene | 82899328 | 82899819 | 82899328 | ID=Msa1000180;Name=Msa1000180 |
| chr6_4 | mRNA | 82899328 | 82899819 | 82899328 | ID=Msa1000180-mRNA-1;Parent=Msa1000180;Name=Msa1000180-mRNA-1;_AED=0.48;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|163 |
| chr6_4 | exon | 82899328 | 82899819 | 82899328 | ID=Msa1000180-mRNA-1:exon:16014;Parent=Msa1000180-mRNA-1 |
| chr6_4 | CDS | 82899328 | 82899819 | 82899328 | ID=Msa1000180-mRNA-1:cds;Parent=Msa1000180-mRNA-1 |
| Gene Sequence |
| Protein sequence |
