AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1000170


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1000170 MtrunA17_Chr6g0486531 85.366 164 24 0 1 164 825 988 4.84e-89 280
Msa1000170 MtrunA17_Chr6g0486531 69.718 142 43 0 22 163 605 746 3.46e-63 209
Msa1000170 MtrunA17_Chr6g0486321 60.000 155 31 2 12 165 25 149 1.65e-50 160
Msa1000170 MtrunA17_Chr6g0486241 80.189 106 7 1 19 124 37 128 8.50e-49 156
Msa1000170 MtrunA17_Chr6g0486491 73.148 108 15 1 43 150 1 94 5.02e-47 148
Msa1000170 MtrunA17_Chr6g0460441 50.000 138 48 1 26 163 13 129 2.14e-38 128
Msa1000170 MtrunA17_Chr6g0486211 86.111 72 10 0 16 87 9 80 1.52e-36 121
Msa1000170 MtrunA17_Chr6g0484381 62.963 108 11 1 57 164 36 114 3.07e-36 123
Msa1000170 MtrunA17_Chr2g0299581 52.703 148 42 4 19 164 4 125 1.30e-35 122
Msa1000170 MtrunA17_Chr2g0299591 92.000 50 4 0 18 67 13 62 1.26e-26 95.9
Msa1000170 MtrunA17_Chr6g0477831 56.962 79 34 0 69 147 11 89 1.55e-25 94.0
Msa1000170 MtrunA17_Chr6g0486281 90.909 44 4 0 121 164 1 44 1.84e-21 82.0
Msa1000170 MtrunA17_Chr6g0486301 51.282 78 28 1 86 163 1033 1100 4.69e-19 83.2
Msa1000170 MtrunA17_Chr6g0448841 47.872 94 48 1 29 122 30 122 1.33e-18 81.3
Msa1000170 MtrunA17_Chr6g0486461 78.049 41 9 0 125 165 10 50 5.00e-15 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1000170 AT4G29060.1 80.272 147 29 0 18 164 749 895 6.11e-74 238
Msa1000170 AT4G29060.1 64.596 161 57 0 4 164 497 657 1.24e-64 213
Msa1000170 AT4G29060.2 64.596 161 51 1 4 164 497 651 6.83e-63 205
Msa1000170 AT4G11120.1 50.538 93 43 2 29 121 76 165 2.63e-18 81.3

Find 39 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGTCTTCTCTTTGCATTTC+AGG 0.133913 6_4:+82883761 None:intergenic
CCTCATTGGTTAAACAATTA+AGG 0.145910 6_4:-82884120 Msa1000170:CDS
TTCCTTCGTCACAATAGTTT+CGG 0.269335 6_4:+82883790 None:intergenic
GCAAGCATACCTCAGGAAAA+AGG 0.286057 6_4:-82884026 Msa1000170:CDS
TCCTTCAGCTGCTAGTCTTC+CGG 0.310289 6_4:+82883979 None:intergenic
TCCTTCGTCACAATAGTTTC+GGG 0.358591 6_4:+82883791 None:intergenic
CTCATTGGTTAAACAATTAA+GGG 0.369404 6_4:-82884119 Msa1000170:CDS
AGTACTGGTTTCGGCCTCAT+TGG 0.405483 6_4:-82884134 Msa1000170:CDS
GCAAGTTGCTGCTTGTCCTC+AGG 0.414009 6_4:-82883840 Msa1000170:CDS
GAAATCAACAGTACTGGTTT+CGG 0.431645 6_4:-82884143 Msa1000170:CDS
AGAAACGGGTGCAGGGATGA+TGG 0.446137 6_4:-82884095 Msa1000170:CDS
TAAACAATTAAGGGAAGAAA+CGG 0.450664 6_4:-82884110 Msa1000170:CDS
TTAAGGGAAGAAACGGGTGC+AGG 0.502165 6_4:-82884103 Msa1000170:CDS
ACATGAGAGAAAATTGTTGA+GGG 0.502839 6_4:-82883721 None:intergenic
TGTCAGGAAATCAACAGTAC+TGG 0.503338 6_4:-82884149 Msa1000170:intron
CTCACTTCTGCCAACAAAGT+CGG 0.506846 6_4:+82883895 None:intergenic
CCTTAATTGTTTAACCAATG+AGG 0.525034 6_4:+82884120 None:intergenic
TGGCAGAAGTGAGAAATTCA+AGG 0.527151 6_4:-82883885 Msa1000170:CDS
TGTTCTCGGGTTTCAATGCA+AGG 0.527406 6_4:+82883741 None:intergenic
AAGTGAGAAATTCAAGGAGT+TGG 0.528224 6_4:-82883879 Msa1000170:CDS
TCATCCGCTGATAAGAAATC+CGG 0.529747 6_4:-82883998 Msa1000170:CDS
TACAGAAGGCGATCTTGAAA+AGG 0.536969 6_4:-82884050 Msa1000170:CDS
AACTGTGAAACCGACTTTGT+TGG 0.542867 6_4:-82883905 Msa1000170:CDS
AACATGAGAGAAAATTGTTG+AGG 0.544870 6_4:-82883722 None:intergenic
AAAAGGCGCAAGCATACCTC+AGG 0.548541 6_4:-82884033 Msa1000170:CDS
ATATCTTCAATAGATACAAA+CGG 0.551658 6_4:+82883815 None:intergenic
CTGAATGTAACCACATATGT+AGG 0.554718 6_4:-82884250 Msa1000170:intron
TACATTCACGATGCACGGAT+CGG 0.559121 6_4:-82883944 Msa1000170:CDS
AAACAATTAAGGGAAGAAAC+GGG 0.568597 6_4:-82884109 Msa1000170:CDS
ACAACTAACACCTACATATG+TGG 0.570358 6_4:+82884240 None:intergenic
GAAGACTAGCAGCTGAAGGA+AGG 0.571916 6_4:-82883976 Msa1000170:CDS
AAAGCTCTTGCTGATACAGA+AGG 0.601688 6_4:-82884064 Msa1000170:CDS
TCCCGAAACTATTGTGACGA+AGG 0.603689 6_4:-82883792 Msa1000170:CDS
TCCGGAAGACTAGCAGCTGA+AGG 0.605549 6_4:-82883980 Msa1000170:CDS
TAAGGGAAGAAACGGGTGCA+GGG 0.607891 6_4:-82884102 Msa1000170:CDS
GTACATACATTCACGATGCA+CGG 0.616607 6_4:-82883949 Msa1000170:CDS
TCTTCCGGATTTCTTATCAG+CGG 0.628603 6_4:+82883994 None:intergenic
TAACACTAACATGTCGACAC+CGG 0.665146 6_4:-82884297 None:intergenic
ATAGATACAAACGGAACCTG+AGG 0.668908 6_4:+82883824 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATCTTCAATAGATACAAA+CGG + chr6_4:82884199-82884218 None:intergenic 20.0%
!!! AATTGTTTTAATGTTCTGTC+AGG - chr6_4:82883846-82883865 Msa1000170:CDS 25.0%
!! CTCATTGGTTAAACAATTAA+GGG - chr6_4:82883892-82883911 Msa1000170:CDS 25.0%
! TAAACAATTAAGGGAAGAAA+CGG - chr6_4:82883901-82883920 Msa1000170:CDS 25.0%
!!! GCATTTTCTGAAATTATCAC+CGG + chr6_4:82883736-82883755 None:intergenic 30.0%
GTTGTGCACAAAAAGAAAAT+TGG - chr6_4:82883789-82883808 Msa1000170:CDS 30.0%
CCTTAATTGTTTAACCAATG+AGG + chr6_4:82883894-82883913 None:intergenic 30.0%
! CCTCATTGGTTAAACAATTA+AGG - chr6_4:82883891-82883910 Msa1000170:CDS 30.0%
AAACAATTAAGGGAAGAAAC+GGG - chr6_4:82883902-82883921 Msa1000170:CDS 30.0%
! ACAATTTTCTCTCATGTTCT+CGG + chr6_4:82884287-82884306 None:intergenic 30.0%
CTGAATGTAACCACATATGT+AGG - chr6_4:82883761-82883780 Msa1000170:CDS 35.0%
ACAACTAACACCTACATATG+TGG + chr6_4:82883774-82883793 None:intergenic 35.0%
!! GAAATCAACAGTACTGGTTT+CGG - chr6_4:82883868-82883887 Msa1000170:CDS 35.0%
AAGTGAGAAATTCAAGGAGT+TGG - chr6_4:82884132-82884151 Msa1000170:CDS 35.0%
TTCCTTCGTCACAATAGTTT+CGG + chr6_4:82884224-82884243 None:intergenic 35.0%
! CAATTTTCTCTCATGTTCTC+GGG + chr6_4:82884286-82884305 None:intergenic 35.0%
TGTCAGGAAATCAACAGTAC+TGG - chr6_4:82883862-82883881 Msa1000170:CDS 40.0%
AAAGCTCTTGCTGATACAGA+AGG - chr6_4:82883947-82883966 Msa1000170:CDS 40.0%
TACAGAAGGCGATCTTGAAA+AGG - chr6_4:82883961-82883980 Msa1000170:CDS 40.0%
CAAGCATACCTCAGGAAAAA+GGG - chr6_4:82883986-82884005 Msa1000170:CDS 40.0%
TCATCCGCTGATAAGAAATC+CGG - chr6_4:82884013-82884032 Msa1000170:CDS 40.0%
TCTTCCGGATTTCTTATCAG+CGG + chr6_4:82884020-82884039 None:intergenic 40.0%
GTACATACATTCACGATGCA+CGG - chr6_4:82884062-82884081 Msa1000170:CDS 40.0%
! AACTGTGAAACCGACTTTGT+TGG - chr6_4:82884106-82884125 Msa1000170:CDS 40.0%
TGGCAGAAGTGAGAAATTCA+AGG - chr6_4:82884126-82884145 Msa1000170:CDS 40.0%
ATAGATACAAACGGAACCTG+AGG + chr6_4:82884190-82884209 None:intergenic 40.0%
TCCTTCGTCACAATAGTTTC+GGG + chr6_4:82884223-82884242 None:intergenic 40.0%
AGGTCTTCTCTTTGCATTTC+AGG + chr6_4:82884253-82884272 None:intergenic 40.0%
GCAAGCATACCTCAGGAAAA+AGG - chr6_4:82883985-82884004 Msa1000170:CDS 45.0%
! ATGAGAGACCCTTTTTCCTG+AGG + chr6_4:82883997-82884016 None:intergenic 45.0%
TACATTCACGATGCACGGAT+CGG - chr6_4:82884067-82884086 Msa1000170:CDS 45.0%
CTCACTTCTGCCAACAAAGT+CGG + chr6_4:82884119-82884138 None:intergenic 45.0%
TCCCGAAACTATTGTGACGA+AGG - chr6_4:82884219-82884238 Msa1000170:intron 45.0%
TGTTCTCGGGTTTCAATGCA+AGG + chr6_4:82884273-82884292 None:intergenic 45.0%
!! AGTACTGGTTTCGGCCTCAT+TGG - chr6_4:82883877-82883896 Msa1000170:CDS 50.0%
TTAAGGGAAGAAACGGGTGC+AGG - chr6_4:82883908-82883927 Msa1000170:CDS 50.0%
TAAGGGAAGAAACGGGTGCA+GGG - chr6_4:82883909-82883928 Msa1000170:CDS 50.0%
! AAAAGGCGCAAGCATACCTC+AGG - chr6_4:82883978-82883997 Msa1000170:CDS 50.0%
!! TCCTTCAGCTGCTAGTCTTC+CGG + chr6_4:82884035-82884054 None:intergenic 50.0%
GAAGACTAGCAGCTGAAGGA+AGG - chr6_4:82884035-82884054 Msa1000170:CDS 50.0%
AGAAACGGGTGCAGGGATGA+TGG - chr6_4:82883916-82883935 Msa1000170:CDS 55.0%
TCCGGAAGACTAGCAGCTGA+AGG - chr6_4:82884031-82884050 Msa1000170:CDS 55.0%
! GCAAGTTGCTGCTTGTCCTC+AGG - chr6_4:82884171-82884190 Msa1000170:intron 55.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 82883724 82884309 82883724 ID=Msa1000170;Name=Msa1000170
chr6_4 mRNA 82883724 82884309 82883724 ID=Msa1000170-mRNA-1;Parent=Msa1000170;Name=Msa1000170-mRNA-1;_AED=0.48;_eAED=0.54;_QI=0|0|0|1|1|1|2|0|166
chr6_4 exon 82884251 82884309 82884251 ID=Msa1000170-mRNA-1:exon:15975;Parent=Msa1000170-mRNA-1
chr6_4 exon 82883724 82884165 82883724 ID=Msa1000170-mRNA-1:exon:15974;Parent=Msa1000170-mRNA-1
chr6_4 CDS 82884251 82884309 82884251 ID=Msa1000170-mRNA-1:cds;Parent=Msa1000170-mRNA-1
chr6_4 CDS 82883724 82884165 82883724 ID=Msa1000170-mRNA-1:cds;Parent=Msa1000170-mRNA-1
Gene Sequence

>Msa1000170

ATGTCGACACCGGTGATAATTTCAGAAAATGCAACAACTGAATGTAACCACATATGTAGGAAATCAACAGTACTGGTTTCGGCCTCATTGGTTAAACAATTAAGGGAAGAAACGGGTGCAGGGATGATGGACTGTAAGAAAGCTCTTGCTGATACAGAAGGCGATCTTGAAAAGGCGCAAGCATACCTCAGGAAAAAGGGTCTCTCATCCGCTGATAAGAAATCCGGAAGACTAGCAGCTGAAGGAAGGATCAGTACATACATTCACGATGCACGGATCGGTGTTCTAATTGAAGTGAACTGTGAAACCGACTTTGTTGGCAGAAGTGAGAAATTCAAGGAGTTGGTTGATGATCTTGCAATGCAAGTTGCTGCTTGTCCTCAGGTTCCGTTTGTATCTATTGAAGATATTCCCGAAACTATTGTGACGAAGGAAAAAGAACCTGAAATGCAAAGAGAAGACCTTGCATTGAAACCCGAGAACATGAGAGAAAATTGTTGA

Protein sequence

>Msa1000170

MSTPVIISENATTECNHICRKSTVLVSASLVKQLREETGAGMMDCKKALADTEGDLEKAQAYLRKKGLSSADKKSGRLAAEGRISTYIHDARIGVLIEVNCETDFVGRSEKFKELVDDLAMQVAACPQVPFVSIEDIPETIVTKEKEPEMQREDLALKPENMRENC*