AlfalfaGEDB | Msa1061060

M. sativa cultivar zhongmu-4 / Msa1061060

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1061060	ACJ83367.1	97.143	35	1	0	59	93	5	39	1.28e-15	76.6

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1061060	sp\|Q94IC4\|FRI2_SOYBN	88.571	35	4	0	59	93	149	183	2.61e-15	71.6

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1061060	B7FFH0	97.143	35	1	0	59	93	5	39	6.14e-16	76.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0014710	Msa1061060	-0.810904	9.140708e-51	-8.615850e-47
Msa0017510	Msa1061060	-0.819534	1.104066e-52	-8.615850e-47
Msa0036920	Msa1061060	-0.818983	1.474228e-52	-8.615850e-47
Msa0039330	Msa1061060	-0.805828	1.105558e-49	-8.615850e-47
Msa0050390	Msa1061060	-0.805118	1.557664e-49	-8.615850e-47
Msa0056530	Msa1061060	-0.800069	1.713171e-48	-8.615850e-47
Msa0078660	Msa1061060	-0.806754	7.055709e-50	-8.615850e-47
Msa0101810	Msa1061060	0.810910	9.113072e-51	-8.615850e-47
Msa0105070	Msa1061060	-0.803989	2.679095e-49	-8.615850e-47
Msa0107640	Msa1061060	-0.813115	3.013489e-51	-8.615850e-47
Msa0613440	Msa1061060	-0.809179	2.150312e-50	-8.615850e-47
Msa0640860	Msa1061060	-0.812672	3.767879e-51	-8.615850e-47
Msa0648880	Msa1061060	-0.822288	2.566770e-53	-8.615850e-47
Msa0654930	Msa1061060	0.819919	9.019467e-53	-8.615850e-47
Msa0657610	Msa1061060	-0.811376	7.221587e-51	-8.615850e-47
Msa0658570	Msa1061060	-0.826028	3.395238e-54	-8.615850e-47
Msa0663380	Msa1061060	-0.808267	3.367536e-50	-8.615850e-47
Msa0672690	Msa1061060	-0.807511	4.878347e-50	-8.615850e-47
Msa0679290	Msa1061060	0.809279	2.046833e-50	-8.615850e-47
Msa0691050	Msa1061060	-0.814557	1.449767e-51	-8.615850e-47
Msa0691380	Msa1061060	-0.814179	1.757185e-51	-8.615850e-47
Msa0724210	Msa1061060	-0.818725	1.687567e-52	-8.615850e-47
Msa0731940	Msa1061060	-0.845125	4.935581e-59	-8.615850e-47
Msa0735170	Msa1061060	-0.820996	5.104145e-53	-8.615850e-47
Msa0756460	Msa1061060	-0.803105	4.086505e-49	-8.615850e-47
Msa0757450	Msa1061060	0.809788	1.591532e-50	-8.615850e-47
Msa0757500	Msa1061060	0.825661	4.151174e-54	-8.615850e-47
Msa0760130	Msa1061060	-0.833744	4.472683e-56	-8.615850e-47
Msa0766630	Msa1061060	-0.805140	1.541042e-49	-8.615850e-47
Msa0768290	Msa1061060	-0.804719	1.887638e-49	-8.615850e-47
Msa0768370	Msa1061060	-0.815576	8.615220e-52	-8.615850e-47
Msa0776990	Msa1061060	-0.814536	1.465699e-51	-8.615850e-47
Msa0787470	Msa1061060	-0.816415	5.595741e-52	-8.615850e-47
Msa0810220	Msa1061060	-0.816597	5.095009e-52	-8.615850e-47
Msa0824590	Msa1061060	-0.802347	5.858263e-49	-8.615850e-47
Msa0834880	Msa1061060	-0.806703	7.232082e-50	-8.615850e-47
Msa0130970	Msa1061060	-0.810142	1.335429e-50	-8.615850e-47
Msa0131770	Msa1061060	-0.812301	4.541182e-51	-8.615850e-47
Msa0133980	Msa1061060	0.808323	3.277013e-50	-8.615850e-47
Msa0157290	Msa1061060	-0.819767	9.769217e-53	-8.615850e-47
Msa0201070	Msa1061060	-0.803582	3.253766e-49	-8.615850e-47
Msa0215320	Msa1061060	0.854729	1.013828e-61	-8.615850e-47
Msa0220740	Msa1061060	-0.835349	1.767578e-56	-8.615850e-47
Msa0225320	Msa1061060	-0.807116	5.913283e-50	-8.615850e-47
Msa0237070	Msa1061060	-0.803052	4.189818e-49	-8.615850e-47
Msa0376590	Msa1061060	-0.812230	4.706157e-51	-8.615850e-47
Msa0381610	Msa1061060	0.802056	6.723694e-49	-8.615850e-47
Msa0388960	Msa1061060	-0.841554	4.429366e-58	-8.615850e-47
Msa0390400	Msa1061060	0.802877	4.553830e-49	-8.615850e-47
Msa0391430	Msa1061060	-0.803087	4.120535e-49	-8.615850e-47
Msa0392180	Msa1061060	-0.835265	1.855290e-56	-8.615850e-47
Msa0395770	Msa1061060	0.817952	2.525516e-52	-8.615850e-47
Msa0395790	Msa1061060	-0.826116	3.236816e-54	-8.615850e-47
Msa0396060	Msa1061060	-0.830841	2.339377e-55	-8.615850e-47
Msa0416650	Msa1061060	-0.811981	5.334812e-51	-8.615850e-47
Msa0443290	Msa1061060	-0.807857	4.118143e-50	-8.615850e-47
Msa0444750	Msa1061060	0.829428	5.177167e-55	-8.615850e-47
Msa0466390	Msa1061060	-0.801050	1.081215e-48	-8.615850e-47
Msa0469940	Msa1061060	0.812873	3.404504e-51	-8.615850e-47
Msa0990960	Msa1061060	-0.809023	2.321920e-50	-8.615850e-47
Msa0992280	Msa1061060	-0.811785	5.883277e-51	-8.615850e-47
Msa0993290	Msa1061060	-0.803033	4.228621e-49	-8.615850e-47
Msa0993600	Msa1061060	-0.800104	1.685066e-48	-8.615850e-47
Msa0998690	Msa1061060	-0.807372	5.219856e-50	-8.615850e-47
Msa1000900	Msa1061060	-0.811410	7.098862e-51	-8.615850e-47
Msa1014540	Msa1061060	-0.840535	8.202075e-58	-8.615850e-47
Msa1015490	Msa1061060	-0.807223	5.614285e-50	-8.615850e-47
Msa1028970	Msa1061060	-0.811329	7.391771e-51	-8.615850e-47
Msa1060980	Msa1061060	0.966557	4.432583e-126	-8.615850e-47
Msa1061020	Msa1061060	0.988642	7.936215e-175	-8.615850e-47
Msa1061060	Msa1068200	0.800582	1.346801e-48	-8.615850e-47
Msa1061060	Msa1075220	0.849728	2.684443e-60	-8.615850e-47
Msa1061060	Msa1075540	-0.802024	6.825602e-49	-8.615850e-47
Msa1061060	Msa1077390	-0.806358	8.551852e-50	-8.615850e-47
Msa1061060	Msa1082580	-0.838633	2.560677e-57	-8.615850e-47
Msa1061060	Msa1090260	-0.802598	5.200390e-49	-8.615850e-47
Msa1061060	Msa1116130	0.827860	1.239034e-54	-8.615850e-47
Msa1061060	Msa1121450	0.812474	4.163032e-51	-8.615850e-47
Msa1061060	Msa1121480	-0.839729	1.330879e-57	-8.615850e-47
Msa1061060	Msa1121490	-0.871374	7.175707e-67	-8.615850e-47
Msa1061060	Msa1137570	-0.828239	1.004250e-54	-8.615850e-47
Msa1061060	Msa1139000	0.837021	6.645147e-57	-8.615850e-47
Msa1061060	Msa1156020	-0.809744	1.626247e-50	-8.615850e-47
Msa1061060	Msa1156340	0.800235	1.585273e-48	-8.615850e-47
Msa1061060	Msa1158310	-0.817109	3.910316e-52	-8.615850e-47
Msa1061060	Msa1162550	0.877870	4.440135e-69	-8.615850e-47
Msa1061060	Msa1178180	-0.810183	1.308164e-50	-8.615850e-47
Msa1061060	Msa1185110	-0.804099	2.541312e-49	-8.615850e-47
Msa1061060	Msa1186950	0.845211	4.677341e-59	-8.615850e-47
Msa1061060	Msa1192130	0.841250	5.324170e-58	-8.615850e-47
Msa1061060	Msa1197290	-0.817681	2.907555e-52	-8.615850e-47
Msa1061060	Msa1205130	-0.806706	7.222829e-50	-8.615850e-47
Msa1061060	Msa1229600	-0.814210	1.730386e-51	-8.615850e-47
Msa1061060	Msa1233890	-0.811926	5.482210e-51	-8.615850e-47
Msa1061060	Msa1238930	-0.809283	2.042235e-50	-8.615850e-47
Msa1061060	Msa1239530	-0.825727	4.002584e-54	-8.615850e-47
Msa1061060	Msa1244250	-0.820548	6.470416e-53	-8.615850e-47
Msa1061060	Msa1247520	0.830889	2.277617e-55	-8.615850e-47
Msa1061060	Msa1249490	-0.819816	9.523619e-53	-8.615850e-47
Msa1061060	Msa1254250	0.808338	3.252786e-50	-8.615850e-47
Msa1061060	Msa1259490	0.801137	1.037870e-48	-8.615850e-47
Msa1061060	Msa1277660	-0.809667	1.689536e-50	-8.615850e-47
Msa1061060	Msa1282580	0.848332	6.556241e-60	-8.615850e-47
Msa1061060	Msa1284680	-0.831277	1.828970e-55	-8.615850e-47
Msa1061060	Msa1286800	-0.803767	2.979139e-49	-8.615850e-47
Msa1061060	Msa1306850	-0.802629	5.122805e-49	-8.615850e-47
Msa1061060	Msa1310210	-0.819750	9.857258e-53	-8.615850e-47
Msa1061060	Msa1313150	-0.837306	5.618836e-57	-8.615850e-47
Msa1061060	Msa1336670	-0.811953	5.410269e-51	-8.615850e-47
Msa1061060	Msa1353470	-0.802629	5.123172e-49	-8.615850e-47
Msa1061060	Msa1359040	-0.823005	1.748229e-53	-8.615850e-47
Msa1061060	Msa1366810	-0.817975	2.494567e-52	-8.615850e-47
Msa1061060	Msa1376890	-0.813639	2.311636e-51	-8.615850e-47
Msa1061060	Msa1379500	-0.803083	4.128189e-49	-8.615850e-47
Msa1061060	Msa1393410	-0.821865	3.217177e-53	-8.615850e-47
Msa1061060	Msa1395530	-0.800283	1.549659e-48	-8.615850e-47
Msa1061060	Msa1420820	-0.810170	1.317066e-50	-8.615850e-47
Msa1061060	Msa1423880	-0.846719	1.820403e-59	-8.615850e-47
Msa1061060	Msa1435420	-0.821031	5.012547e-53	-8.615850e-47
Msa1061060	Msa1445610	0.807361	5.248832e-50	-8.615850e-47
Msa1061060	Msa1448640	-0.816993	4.151970e-52	-8.615850e-47
Msa1061060	Msa1451620	0.822828	1.922074e-53	-8.615850e-47
Msa1061060	Msa1453320	-0.819234	1.292809e-52	-8.615850e-47
Msa1061060	Msa1457040	-0.817955	2.521591e-52	-8.615850e-47
Msa1061060	Msa1466540	-0.817215	3.701082e-52	-8.615850e-47
Msa0251640	Msa1061060	-0.807893	4.047150e-50	-8.615850e-47
Msa0269810	Msa1061060	-0.831538	1.577164e-55	-8.615850e-47
Msa0273100	Msa1061060	-0.804928	1.707085e-49	-8.615850e-47
Msa0278190	Msa1061060	-0.816389	5.669958e-52	-8.615850e-47
Msa0288100	Msa1061060	-0.801918	7.176057e-49	-8.615850e-47
Msa0305190	Msa1061060	-0.826334	2.871348e-54	-8.615850e-47
Msa0340450	Msa1061060	-0.834680	2.605480e-56	-8.615850e-47
Msa0344690	Msa1061060	0.822144	2.771379e-53	-8.615850e-47
Msa0350990	Msa1061060	-0.816410	5.611458e-52	-8.615850e-47
Msa0351030	Msa1061060	-0.823364	1.441078e-53	-8.615850e-47
Msa0490730	Msa1061060	0.804919	1.714558e-49	-8.615850e-47
Msa0490870	Msa1061060	-0.811139	8.128360e-51	-8.615850e-47
Msa0516370	Msa1061060	-0.810467	1.136038e-50	-8.615850e-47
Msa0517110	Msa1061060	-0.804491	2.105780e-49	-8.615850e-47
Msa0555020	Msa1061060	-0.811350	7.314460e-51	-8.615850e-47
Msa0574760	Msa1061060	0.829332	5.461828e-55	-8.615850e-47
Msa0585000	Msa1061060	0.809883	1.517831e-50	-8.615850e-47
Msa0585230	Msa1061060	-0.813697	2.244746e-51	-8.615850e-47
Msa0844490	Msa1061060	-0.813168	2.934270e-51	-8.615850e-47
Msa0870530	Msa1061060	-0.803126	4.045220e-49	-8.615850e-47
Msa0880140	Msa1061060	-0.817729	2.834678e-52	-8.615850e-47
Msa0891040	Msa1061060	-0.804540	2.057149e-49	-8.615850e-47
Msa0907070	Msa1061060	-0.817213	3.705502e-52	-8.615850e-47
Msa0908770	Msa1061060	0.946028	1.006490e-104	-8.615850e-47
Msa0914270	Msa1061060	-0.805717	1.166399e-49	-8.615850e-47
Msa0919850	Msa1061060	-0.808599	2.861059e-50	-8.615850e-47
Msa0926890	Msa1061060	-0.807736	4.368565e-50	-8.615850e-47
Msa0927380	Msa1061060	-0.812108	5.003356e-51	-8.615850e-47
Msa0930910	Msa1061060	0.800406	1.462966e-48	-8.615850e-47
Msa0931520	Msa1061060	-0.801203	1.005745e-48	-8.615850e-47
Msa0936300	Msa1061060	-0.808410	3.139856e-50	-8.615850e-47
Msa0942200	Msa1061060	-0.815478	9.056801e-52	-8.615850e-47
Msa0947660	Msa1061060	-0.803544	3.314183e-49	-8.615850e-47
Msa0954130	Msa1061060	-0.816433	5.545423e-52	-8.615850e-47
Msa0954550	Msa1061060	-0.808237	3.418786e-50	-8.615850e-47
Msa0962340	Msa1061060	-0.804339	2.265333e-49	-8.615850e-47
Msa0966750	Msa1061060	-0.804485	2.112173e-49	-8.615850e-47
Msa0967180	Msa1061060	-0.818380	2.019830e-52	-8.615850e-47
Msa0969450	Msa1061060	-0.803444	3.475193e-49	-8.615850e-47
Msa0969520	Msa1061060	-0.835134	2.002505e-56	-8.615850e-47
Msa0974080	Msa1061060	-0.813763	2.170508e-51	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1061060	MtrunA17_Chr4g0005581	97.143	35	1	0	59	93	147	181	1.57e-18	77.0
Msa1061060	MtrunA17_Chr7g0243241	75.000	36	9	0	58	93	147	182	1.14e-13	64.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1061060	AT5G01600.1	85.714	35	5	0	59	93	152	186	1.55e-14	67.0
Msa1061060	AT3G56090.1	75.000	36	9	0	58	93	152	187	6.44e-12	60.1

Find 72 sgRNAs with CRISPR-Local

Find 306 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGCACAAAGTTCTTTAAATT+TGG	0.116564	7_2:+49997465	None:intergenic
TTGGAGTAGAAGAAATATTT+TGG	0.229234	7_2:-49997576	Msa1061060:three_prime_UTR
TCTTCACAAGGGAGGATCTT+TGG	0.259240	7_2:+49997342	None:intergenic
TGGTTGTATCCATGAATATT+TGG	0.261284	7_2:-49997556	Msa1061060:three_prime_UTR
AAGTTTCAATTTGACAATTA+AGG	0.262997	7_2:+49997619	None:intergenic
AATTGTATTGATAACAAATT+AGG	0.264616	7_2:+49997065	None:intergenic
GCCATGGAATTGGCTGTCTT+TGG	0.267558	7_2:-49997891	Msa1061060:CDS
CTGAACCCATGCTACGTAAC+TGG	0.301815	7_2:-49997171	Msa1061060:three_prime_UTR
TCGATGAAGTCTGCCAATTA+AGG	0.322512	7_2:+49997674	None:intergenic
TCGTAACAATGATCCTTAAT+TGG	0.332568	7_2:-49997687	Msa1061060:three_prime_UTR
AGGATCTTTGGAGTGTGGGC+AGG	0.340220	7_2:+49997354	None:intergenic
TTGGAGTGTGGGCAGGGCTT+TGG	0.344575	7_2:+49997361	None:intergenic
GCCTTCAAAACAAGTGAATA+TGG	0.347432	7_2:-49997196	Msa1061060:three_prime_UTR
AGAATTTGATCATGCAGAAA+AGG	0.391733	7_2:-49998141	Msa1061060:CDS
TCTGAGGGAGGGCTCACAAT+AGG	0.396058	7_2:+49998161	None:intergenic
AAACATCTACCAAATATTCA+TGG	0.397452	7_2:+49997547	None:intergenic
AGAAGCCTCAAGAGCGGATG+AGG	0.415926	7_2:-49997310	Msa1061060:three_prime_UTR
GGATCTTTGGAGTGTGGGCA+GGG	0.444116	7_2:+49997355	None:intergenic
CTGAGGGAGGGCTCACAATA+GGG	0.446437	7_2:+49998162	None:intergenic
ATTACTTGGATGATATTACT+TGG	0.456112	7_2:-49997520	Msa1061060:three_prime_UTR
GAAATTCCAGTTACGTAGCA+TGG	0.479187	7_2:+49997165	None:intergenic
GGGCTTTGGTGTAACATTGA+AGG	0.483853	7_2:+49997375	None:intergenic
TCCTCCTCTGTCTCTAGCTC+CGG	0.487582	7_2:-49999223	Msa1061060:CDS
CTAAACACATGTCCCTCAAA+AGG	0.491007	7_2:+49997119	None:intergenic
GGATTGACGGTGGGTTTGAT+CGG	0.493933	7_2:+49999178	None:intergenic
ATTGTTTCAACATTGTGGAC+AGG	0.496676	7_2:-49997439	Msa1061060:three_prime_UTR
CCTCCCTTGTGAAGAAGTCT+AGG	0.513526	7_2:-49997334	Msa1061060:three_prime_UTR
ATTGACTTAGCTTTGCTCGT+TGG	0.519060	7_2:-49997402	Msa1061060:three_prime_UTR
GAATTTGATCATGCAGAAAA+GGG	0.523661	7_2:-49998140	Msa1061060:CDS
ACCCACCGTCAATCCTTCTC+CGG	0.524828	7_2:-49999170	Msa1061060:intron
GAGGATACGTCGATCGGAGA+TGG	0.528014	7_2:+49999241	None:intergenic
TGATTTGTTTAGAACATTCG+AGG	0.529198	7_2:-49998203	Msa1061060:intron
CAGAGATTGTTTCAACATTG+TGG	0.529476	7_2:-49997444	Msa1061060:three_prime_UTR
GGCTTTGGTGTAACATTGAA+GGG	0.530378	7_2:+49997376	None:intergenic
ATCCACCGGAGAAGGATTGA+CGG	0.532528	7_2:+49999165	None:intergenic
AAATTCCAGTTACGTAGCAT+GGG	0.532895	7_2:+49997166	None:intergenic
AGGGCGCACGGAGGAAGTGA+TGG	0.541491	7_2:+49999273	None:intergenic
TTCTGCATGATCAAATTCTG+AGG	0.542218	7_2:+49998145	None:intergenic
ATCGGAGATGGTGGAAGAAG+AGG	0.542512	7_2:+49999253	None:intergenic
GATACGTCGATCGGAGATGG+TGG	0.543439	7_2:+49999244	None:intergenic
AGGGAGGATCTTTGGAGTGT+GGG	0.547566	7_2:+49997350	None:intergenic
TCCTCTGTCTCTAGCTCCGG+TGG	0.547712	7_2:-49999220	Msa1061060:CDS
AAGACCCTCATCCGCTCTTG+AGG	0.548029	7_2:+49997305	None:intergenic
TCTGCATGATCAAATTCTGA+GGG	0.551161	7_2:+49998146	None:intergenic
CGGAGGAGATAGAGATCCAC+CGG	0.554678	7_2:+49999204	None:intergenic
TCGGAGATGGTGGAAGAAGA+GGG	0.554770	7_2:+49999254	None:intergenic
AGAGCGGATGAGGGTCTTGA+CGG	0.560421	7_2:-49997300	Msa1061060:three_prime_UTR
TCCAAAGACAGCCAATTCCA+TGG	0.569848	7_2:+49997890	None:intergenic
TGGTGGAAGAAGAGGGCGCA+CGG	0.573169	7_2:+49999261	None:intergenic
GATCAGAGATCCACCGGAGA+AGG	0.580807	7_2:+49999157	None:intergenic
GAAAAGGGAGATGCATTGTA+TGG	0.589420	7_2:-49998125	Msa1061060:intron
AAGGGAGGATCTTTGGAGTG+TGG	0.591066	7_2:+49997349	None:intergenic
ACAGAGGAGGATACGTCGAT+CGG	0.603335	7_2:+49999235	None:intergenic
CATGATCAAATTCTGAGGGA+GGG	0.603900	7_2:+49998150	None:intergenic
ACCGGAGAAGGATTGACGGT+GGG	0.606201	7_2:+49999169	None:intergenic
ACGGTGGGTTTGATCGGAGA+CGG	0.609139	7_2:+49999184	None:intergenic
GCATGATCAAATTCTGAGGG+AGG	0.609988	7_2:+49998149	None:intergenic
GCGCACGGAGGAAGTGATGG+TGG	0.610315	7_2:+49999276	None:intergenic
CTAGGCAGAAGCCTCAAGAG+CGG	0.638849	7_2:-49997316	Msa1061060:three_prime_UTR
AAGTAATTCAACATCAAGAG+AGG	0.643005	7_2:+49997096	None:intergenic
GAACATTCGAGGTGGAAGAG+TGG	0.645020	7_2:-49998192	Msa1061060:CDS
AAAGCTAAGTCAATGAAGAG+AGG	0.649380	7_2:+49997411	None:intergenic
TCCACCGGAGCTAGAGACAG+AGG	0.651482	7_2:+49999219	None:intergenic
GAAGCCTCAAGAGCGGATGA+GGG	0.661946	7_2:-49997309	Msa1061060:three_prime_UTR
CCTAGACTTCTTCACAAGGG+AGG	0.667480	7_2:+49997334	None:intergenic
ACCGGAGCTAGAGACAGAGG+AGG	0.671704	7_2:+49999222	None:intergenic
CACCGGAGAAGGATTGACGG+TGG	0.685682	7_2:+49999168	None:intergenic
TCTGCCTAGACTTCTTCACA+AGG	0.686387	7_2:+49997330	None:intergenic
GTGGGTTTGATCGGAGACGG+AGG	0.686516	7_2:+49999187	None:intergenic
TTTGTTTAGAACATTCGAGG+TGG	0.691243	7_2:-49998200	Msa1061060:intron
CTGCCTAGACTTCTTCACAA+GGG	0.694383	7_2:+49997331	None:intergenic
TGGAAGAAGAGGGCGCACGG+AGG	0.751026	7_2:+49999264	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATAATTAACAAAAAGATTAA+AGG	+	chr7_2:49997629-49997648	None:intergenic	10.0%
!!	ATAATTAACAAAAAGATTAA+AGG	+	chr7_2:49997629-49997648	None:intergenic	10.0%
!!	TTATGTTTCATGAATATAAT+AGG	-	chr7_2:49998679-49998698	Msa1061060:intron	15.0%
!!	TTATGTTTCATGAATATAAT+AGG	-	chr7_2:49998679-49998698	Msa1061060:intron	15.0%
!!	TTGTAAAATCTAGCATAATT+AGG	-	chr7_2:49997552-49997571	Msa1061060:three_prime_UTR	20.0%
!!	GATAATTAAATATTCAGCAA+TGG	-	chr7_2:49997733-49997752	Msa1061060:intron	20.0%
!!	TGATAAGATATCATAGTTTA+TGG	-	chr7_2:49997934-49997953	Msa1061060:intron	20.0%
!!	ATATGATAAATCAAAACAGA+GGG	+	chr7_2:49998336-49998355	None:intergenic	20.0%
!!	AATATGATAAATCAAAACAG+AGG	+	chr7_2:49998337-49998356	None:intergenic	20.0%
!!!	TGATAATAGTTTTGAGAATA+TGG	-	chr7_2:49998402-49998421	Msa1061060:intron	20.0%
!!	ATATACATATTGAGATCAAA+TGG	-	chr7_2:49998640-49998659	Msa1061060:intron	20.0%
!!	AAGTTTCAATTTGACAATTA+AGG	+	chr7_2:49998780-49998799	None:intergenic	20.0%
!!!	AAATCATTTTTCTGTATACT+TGG	-	chr7_2:49998801-49998820	Msa1061060:intron	20.0%
!!	AAAGGAAAAAAGAAAAAAAC+AGG	+	chr7_2:49999262-49999281	None:intergenic	20.0%
!!!	TTTTTTCTTTTTTCCTTTTG+AGG	-	chr7_2:49999264-49999283	Msa1061060:CDS	20.0%
!!!	TTTTTCTTTTTTCCTTTTGA+GGG	-	chr7_2:49999265-49999284	Msa1061060:CDS	20.0%
!!	TTGTAAAATCTAGCATAATT+AGG	-	chr7_2:49997552-49997571	Msa1061060:three_prime_UTR	20.0%
!!	GATAATTAAATATTCAGCAA+TGG	-	chr7_2:49997733-49997752	Msa1061060:intron	20.0%
!!	TGATAAGATATCATAGTTTA+TGG	-	chr7_2:49997934-49997953	Msa1061060:intron	20.0%
!!	ATATGATAAATCAAAACAGA+GGG	+	chr7_2:49998336-49998355	None:intergenic	20.0%
!!	AATATGATAAATCAAAACAG+AGG	+	chr7_2:49998337-49998356	None:intergenic	20.0%
!!!	TGATAATAGTTTTGAGAATA+TGG	-	chr7_2:49998402-49998421	Msa1061060:intron	20.0%
!!	ATATACATATTGAGATCAAA+TGG	-	chr7_2:49998640-49998659	Msa1061060:intron	20.0%
!!	AAGTTTCAATTTGACAATTA+AGG	+	chr7_2:49998780-49998799	None:intergenic	20.0%
!!!	AAATCATTTTTCTGTATACT+TGG	-	chr7_2:49998801-49998820	Msa1061060:intron	20.0%
!!	AAAGGAAAAAAGAAAAAAAC+AGG	+	chr7_2:49999262-49999281	None:intergenic	20.0%
!!!	TTTTTTCTTTTTTCCTTTTG+AGG	-	chr7_2:49999264-49999283	Msa1061060:CDS	20.0%
!!!	TTTTTCTTTTTTCCTTTTGA+GGG	-	chr7_2:49999265-49999284	Msa1061060:CDS	20.0%
!	TGATCTGTATGAAATTGAAT+TGG	-	chr7_2:49997666-49997685	Msa1061060:three_prime_UTR	25.0%
!	GTATGAAATTGAATTGGTTA+TGG	-	chr7_2:49997672-49997691	Msa1061060:three_prime_UTR	25.0%
!!!	TATTTTTGTTATTTGGTCTC+TGG	-	chr7_2:49997766-49997785	Msa1061060:intron	25.0%
!	TAGCATACAATTTCATTTGA+AGG	-	chr7_2:49997896-49997915	Msa1061060:intron	25.0%
!	AGCATACAATTTCATTTGAA+GGG	-	chr7_2:49997897-49997916	Msa1061060:intron	25.0%
!	AAGATATCATAGTTTATGGA+TGG	-	chr7_2:49997938-49997957	Msa1061060:intron	25.0%
!	ATATATGGAAATTAGATGCA+TGG	-	chr7_2:49998130-49998149	Msa1061060:CDS	25.0%
!	TATATGGAAATTAGATGCAT+GGG	-	chr7_2:49998131-49998150	Msa1061060:CDS	25.0%
!	TTATTGCATGAGAAAAAACA+GGG	+	chr7_2:49998603-49998622	None:intergenic	25.0%
!	TTTATTGCATGAGAAAAAAC+AGG	+	chr7_2:49998604-49998623	None:intergenic	25.0%
!	TGATCTCAATATGTATATAC+AGG	+	chr7_2:49998638-49998657	None:intergenic	25.0%
!	TGTTTCATGAATATAATAGG+TGG	-	chr7_2:49998682-49998701	Msa1061060:intron	25.0%
!!	TTGGAGTAGAAGAAATATTT+TGG	-	chr7_2:49998820-49998839	Msa1061060:intron	25.0%
!	AAACATCTACCAAATATTCA+TGG	+	chr7_2:49998852-49998871	None:intergenic	25.0%
!!!	GTAGATGTTTTTAGATTACT+TGG	-	chr7_2:49998862-49998881	Msa1061060:intron	25.0%
!	ATTACTTGGATGATATTACT+TGG	-	chr7_2:49998876-49998895	Msa1061060:intron	25.0%
!	TGCACAAAGTTCTTTAAATT+TGG	+	chr7_2:49998934-49998953	None:intergenic	25.0%
!!!	AATATCATTTGCTGATTTAC+AGG	-	chr7_2:49999178-49999197	Msa1061060:CDS	25.0%
!	TGATCTGTATGAAATTGAAT+TGG	-	chr7_2:49997666-49997685	Msa1061060:three_prime_UTR	25.0%
!	GTATGAAATTGAATTGGTTA+TGG	-	chr7_2:49997672-49997691	Msa1061060:three_prime_UTR	25.0%
!!!	TATTTTTGTTATTTGGTCTC+TGG	-	chr7_2:49997766-49997785	Msa1061060:intron	25.0%
!	TAGCATACAATTTCATTTGA+AGG	-	chr7_2:49997896-49997915	Msa1061060:intron	25.0%
!	AGCATACAATTTCATTTGAA+GGG	-	chr7_2:49997897-49997916	Msa1061060:intron	25.0%
!	AAGATATCATAGTTTATGGA+TGG	-	chr7_2:49997938-49997957	Msa1061060:intron	25.0%
!	ATATATGGAAATTAGATGCA+TGG	-	chr7_2:49998130-49998149	Msa1061060:CDS	25.0%
!	TATATGGAAATTAGATGCAT+GGG	-	chr7_2:49998131-49998150	Msa1061060:CDS	25.0%
!	TTATTGCATGAGAAAAAACA+GGG	+	chr7_2:49998603-49998622	None:intergenic	25.0%
!	TTTATTGCATGAGAAAAAAC+AGG	+	chr7_2:49998604-49998623	None:intergenic	25.0%
!	TGATCTCAATATGTATATAC+AGG	+	chr7_2:49998638-49998657	None:intergenic	25.0%
!	TGTTTCATGAATATAATAGG+TGG	-	chr7_2:49998682-49998701	Msa1061060:intron	25.0%
!!	TTGGAGTAGAAGAAATATTT+TGG	-	chr7_2:49998820-49998839	Msa1061060:intron	25.0%
!	AAACATCTACCAAATATTCA+TGG	+	chr7_2:49998852-49998871	None:intergenic	25.0%
!!!	GTAGATGTTTTTAGATTACT+TGG	-	chr7_2:49998862-49998881	Msa1061060:intron	25.0%
!	ATTACTTGGATGATATTACT+TGG	-	chr7_2:49998876-49998895	Msa1061060:intron	25.0%
!	TGCACAAAGTTCTTTAAATT+TGG	+	chr7_2:49998934-49998953	None:intergenic	25.0%
!!!	AATATCATTTGCTGATTTAC+AGG	-	chr7_2:49999178-49999197	Msa1061060:CDS	25.0%
	ATTGGTTATGGAAGTTGAAT+TGG	-	chr7_2:49997684-49997703	Msa1061060:three_prime_UTR	30.0%
	ATAGTTTATGGATGGTTAAC+AGG	-	chr7_2:49997946-49997965	Msa1061060:intron	30.0%
!	TTTGTGGTCATCAATTTTGA+TGG	-	chr7_2:49997970-49997989	Msa1061060:intron	30.0%
	ATAGTGTATGGATGGTTAAT+AGG	-	chr7_2:49998019-49998038	Msa1061060:intron	30.0%
!!!	TAGGTTTTGATTTGTTCCTT+TGG	-	chr7_2:49998038-49998057	Msa1061060:intron	30.0%
	TCATATCCTAGTTTCCTAAT+GGG	+	chr7_2:49998090-49998109	None:intergenic	30.0%
	TTCATATCCTAGTTTCCTAA+TGG	+	chr7_2:49998091-49998110	None:intergenic	30.0%
	TACTGACAGAGAAAGATATA+TGG	-	chr7_2:49998115-49998134	Msa1061060:intron	30.0%
!!	TGATTTGTTTAGAACATTCG+AGG	-	chr7_2:49998193-49998212	Msa1061060:CDS	30.0%
	AGAATTTGATCATGCAGAAA+AGG	-	chr7_2:49998255-49998274	Msa1061060:intron	30.0%
	GAATTTGATCATGCAGAAAA+GGG	-	chr7_2:49998256-49998275	Msa1061060:intron	30.0%
	AACAAGGCAAATGAAAATAG+AGG	+	chr7_2:49998307-49998326	None:intergenic	30.0%
	AAACAGAGGGAAAAAAAACA+AGG	+	chr7_2:49998323-49998342	None:intergenic	30.0%
!!!	TTTTTGTTTTGATTCAGCCA+TGG	-	chr7_2:49998489-49998508	Msa1061060:intron	30.0%
	TCGTAACAATGATCCTTAAT+TGG	-	chr7_2:49998709-49998728	Msa1061060:intron	30.0%
	TGGTTGTATCCATGAATATT+TGG	-	chr7_2:49998840-49998859	Msa1061060:intron	30.0%
!!	TAAATTTGGAGTTTTCGACA+CGG	+	chr7_2:49998920-49998939	None:intergenic	30.0%
	AAGTAATTCAACATCAAGAG+AGG	+	chr7_2:49999303-49999322	None:intergenic	30.0%
	ATTGGTTATGGAAGTTGAAT+TGG	-	chr7_2:49997684-49997703	Msa1061060:three_prime_UTR	30.0%
	ATAGTTTATGGATGGTTAAC+AGG	-	chr7_2:49997946-49997965	Msa1061060:intron	30.0%
!	TTTGTGGTCATCAATTTTGA+TGG	-	chr7_2:49997970-49997989	Msa1061060:intron	30.0%
	ATAGTGTATGGATGGTTAAT+AGG	-	chr7_2:49998019-49998038	Msa1061060:intron	30.0%
!!!	TAGGTTTTGATTTGTTCCTT+TGG	-	chr7_2:49998038-49998057	Msa1061060:intron	30.0%
	TCATATCCTAGTTTCCTAAT+GGG	+	chr7_2:49998090-49998109	None:intergenic	30.0%
	TTCATATCCTAGTTTCCTAA+TGG	+	chr7_2:49998091-49998110	None:intergenic	30.0%
	TACTGACAGAGAAAGATATA+TGG	-	chr7_2:49998115-49998134	Msa1061060:intron	30.0%
!!	TGATTTGTTTAGAACATTCG+AGG	-	chr7_2:49998193-49998212	Msa1061060:CDS	30.0%
	AGAATTTGATCATGCAGAAA+AGG	-	chr7_2:49998255-49998274	Msa1061060:intron	30.0%
	GAATTTGATCATGCAGAAAA+GGG	-	chr7_2:49998256-49998275	Msa1061060:intron	30.0%
	AACAAGGCAAATGAAAATAG+AGG	+	chr7_2:49998307-49998326	None:intergenic	30.0%
	AAACAGAGGGAAAAAAAACA+AGG	+	chr7_2:49998323-49998342	None:intergenic	30.0%
!!!	TTTTTGTTTTGATTCAGCCA+TGG	-	chr7_2:49998489-49998508	Msa1061060:intron	30.0%
	TCGTAACAATGATCCTTAAT+TGG	-	chr7_2:49998709-49998728	Msa1061060:intron	30.0%
	TGGTTGTATCCATGAATATT+TGG	-	chr7_2:49998840-49998859	Msa1061060:intron	30.0%
!!	TAAATTTGGAGTTTTCGACA+CGG	+	chr7_2:49998920-49998939	None:intergenic	30.0%
	AAGTAATTCAACATCAAGAG+AGG	+	chr7_2:49999303-49999322	None:intergenic	30.0%
!	AGAAGGATGCAATTGATGAT+TGG	+	chr7_2:49997290-49997309	None:intergenic	35.0%
	ACATGCAGTAACTCTGTTAA+TGG	-	chr7_2:49997794-49997813	Msa1061060:intron	35.0%
	CTCTGTTCATTCATAGTGTA+TGG	-	chr7_2:49998007-49998026	Msa1061060:intron	35.0%
	GTTCATTCATAGTGTATGGA+TGG	-	chr7_2:49998011-49998030	Msa1061060:intron	35.0%
!!!	TTTTGATTTGTTCCTTTGGC+AGG	-	chr7_2:49998042-49998061	Msa1061060:intron	35.0%
!!	TTTGATTTGTTCCTTTGGCA+GGG	-	chr7_2:49998043-49998062	Msa1061060:intron	35.0%
	TTTCTTGGATGAATCCCATT+AGG	-	chr7_2:49998073-49998092	Msa1061060:intron	35.0%
	ATGAATCCCATTAGGAAACT+AGG	-	chr7_2:49998081-49998100	Msa1061060:intron	35.0%
!	TTTGTTTAGAACATTCGAGG+TGG	-	chr7_2:49998196-49998215	Msa1061060:intron	35.0%
	TCTGCATGATCAAATTCTGA+GGG	+	chr7_2:49998253-49998272	None:intergenic	35.0%
	TTCTGCATGATCAAATTCTG+AGG	+	chr7_2:49998254-49998273	None:intergenic	35.0%
!!	TTTTGATTCAGCCATGGAAT+TGG	-	chr7_2:49998495-49998514	Msa1061060:intron	35.0%
	ATCCAAATACAGAGAGAAAG+AGG	+	chr7_2:49998575-49998594	None:intergenic	35.0%
	CAGAGATTGTTTCAACATTG+TGG	-	chr7_2:49998952-49998971	Msa1061060:intron	35.0%
	ATTGTTTCAACATTGTGGAC+AGG	-	chr7_2:49998957-49998976	Msa1061060:intron	35.0%
	AAAGCTAAGTCAATGAAGAG+AGG	+	chr7_2:49998988-49999007	None:intergenic	35.0%
	GCCTTCAAAACAAGTGAATA+TGG	-	chr7_2:49999200-49999219	Msa1061060:CDS	35.0%
!!!	GCCATATTCACTTGTTTTGA+AGG	+	chr7_2:49999204-49999223	None:intergenic	35.0%
	AAATTCCAGTTACGTAGCAT+GGG	+	chr7_2:49999233-49999252	None:intergenic	35.0%
!	AGAAGGATGCAATTGATGAT+TGG	+	chr7_2:49997290-49997309	None:intergenic	35.0%
	ACATGCAGTAACTCTGTTAA+TGG	-	chr7_2:49997794-49997813	Msa1061060:intron	35.0%
	CTCTGTTCATTCATAGTGTA+TGG	-	chr7_2:49998007-49998026	Msa1061060:intron	35.0%
	GTTCATTCATAGTGTATGGA+TGG	-	chr7_2:49998011-49998030	Msa1061060:intron	35.0%
!!!	TTTTGATTTGTTCCTTTGGC+AGG	-	chr7_2:49998042-49998061	Msa1061060:intron	35.0%
!!	TTTGATTTGTTCCTTTGGCA+GGG	-	chr7_2:49998043-49998062	Msa1061060:intron	35.0%
	TTTCTTGGATGAATCCCATT+AGG	-	chr7_2:49998073-49998092	Msa1061060:intron	35.0%
	ATGAATCCCATTAGGAAACT+AGG	-	chr7_2:49998081-49998100	Msa1061060:intron	35.0%
!	TTTGTTTAGAACATTCGAGG+TGG	-	chr7_2:49998196-49998215	Msa1061060:intron	35.0%
	TCTGCATGATCAAATTCTGA+GGG	+	chr7_2:49998253-49998272	None:intergenic	35.0%
	TTCTGCATGATCAAATTCTG+AGG	+	chr7_2:49998254-49998273	None:intergenic	35.0%
!!	TTTTGATTCAGCCATGGAAT+TGG	-	chr7_2:49998495-49998514	Msa1061060:intron	35.0%
	ATCCAAATACAGAGAGAAAG+AGG	+	chr7_2:49998575-49998594	None:intergenic	35.0%
	CAGAGATTGTTTCAACATTG+TGG	-	chr7_2:49998952-49998971	Msa1061060:intron	35.0%
	ATTGTTTCAACATTGTGGAC+AGG	-	chr7_2:49998957-49998976	Msa1061060:intron	35.0%
	AAAGCTAAGTCAATGAAGAG+AGG	+	chr7_2:49998988-49999007	None:intergenic	35.0%
	GCCTTCAAAACAAGTGAATA+TGG	-	chr7_2:49999200-49999219	Msa1061060:CDS	35.0%
!!!	GCCATATTCACTTGTTTTGA+AGG	+	chr7_2:49999204-49999223	None:intergenic	35.0%
	AAATTCCAGTTACGTAGCAT+GGG	+	chr7_2:49999233-49999252	None:intergenic	35.0%
	TCATCAATTGCATCCTTCTG+TGG	-	chr7_2:49997291-49997310	Msa1061060:intron	40.0%
	GGAGATTGAGAAGTTTCGAT+CGG	+	chr7_2:49997369-49997388	None:intergenic	40.0%
!	ATCGCTGATTGAATTGCGAT+CGG	+	chr7_2:49997438-49997457	None:intergenic	40.0%
!	TGGATGGTTAACAGGTTTTG+TGG	-	chr7_2:49997954-49997973	Msa1061060:intron	40.0%
	GAAAAAACTATCCCTGCCAA+AGG	+	chr7_2:49998057-49998076	None:intergenic	40.0%
!!!	TGGCAGGGATAGTTTTTTCT+TGG	-	chr7_2:49998058-49998077	Msa1061060:intron	40.0%
!	CATGATCAAATTCTGAGGGA+GGG	+	chr7_2:49998249-49998268	None:intergenic	40.0%
	GAAAAGGGAGATGCATTGTA+TGG	-	chr7_2:49998271-49998290	Msa1061060:intron	40.0%
	TGCCTCTTTCTCTCTGTATT+TGG	-	chr7_2:49998570-49998589	Msa1061060:intron	40.0%
	TCGATGAAGTCTGCCAATTA+AGG	+	chr7_2:49998725-49998744	None:intergenic	40.0%
!!	ATTGACTTAGCTTTGCTCGT+TGG	-	chr7_2:49998994-49999013	Msa1061060:intron	40.0%
!	GGCTTTGGTGTAACATTGAA+GGG	+	chr7_2:49999023-49999042	None:intergenic	40.0%
	GAAATTCCAGTTACGTAGCA+TGG	+	chr7_2:49999234-49999253	None:intergenic	40.0%
	CTAAACACATGTCCCTCAAA+AGG	+	chr7_2:49999280-49999299	None:intergenic	40.0%
	TCATCAATTGCATCCTTCTG+TGG	-	chr7_2:49997291-49997310	Msa1061060:intron	40.0%
	GGAGATTGAGAAGTTTCGAT+CGG	+	chr7_2:49997369-49997388	None:intergenic	40.0%
!	ATCGCTGATTGAATTGCGAT+CGG	+	chr7_2:49997438-49997457	None:intergenic	40.0%
!	TGGATGGTTAACAGGTTTTG+TGG	-	chr7_2:49997954-49997973	Msa1061060:intron	40.0%
	GAAAAAACTATCCCTGCCAA+AGG	+	chr7_2:49998057-49998076	None:intergenic	40.0%
!!!	TGGCAGGGATAGTTTTTTCT+TGG	-	chr7_2:49998058-49998077	Msa1061060:intron	40.0%
!	CATGATCAAATTCTGAGGGA+GGG	+	chr7_2:49998249-49998268	None:intergenic	40.0%
	GAAAAGGGAGATGCATTGTA+TGG	-	chr7_2:49998271-49998290	Msa1061060:intron	40.0%
	TGCCTCTTTCTCTCTGTATT+TGG	-	chr7_2:49998570-49998589	Msa1061060:intron	40.0%
	TCGATGAAGTCTGCCAATTA+AGG	+	chr7_2:49998725-49998744	None:intergenic	40.0%
!!	ATTGACTTAGCTTTGCTCGT+TGG	-	chr7_2:49998994-49999013	Msa1061060:intron	40.0%
!	GGCTTTGGTGTAACATTGAA+GGG	+	chr7_2:49999023-49999042	None:intergenic	40.0%
	GAAATTCCAGTTACGTAGCA+TGG	+	chr7_2:49999234-49999253	None:intergenic	40.0%
	CTAAACACATGTCCCTCAAA+AGG	+	chr7_2:49999280-49999299	None:intergenic	40.0%
	TCAATTGCATCCTTCTGTGG+TGG	-	chr7_2:49997294-49997313	Msa1061060:intron	45.0%
	TGAGAAGTTTCGATCGGAAG+AGG	+	chr7_2:49997363-49997382	None:intergenic	45.0%
!	GCATGATCAAATTCTGAGGG+AGG	+	chr7_2:49998250-49998269	None:intergenic	45.0%
	TCCAAAGACAGCCAATTCCA+TGG	+	chr7_2:49998509-49998528	None:intergenic	45.0%
!	CTTCATCGAGAGCGAGTTTT+TGG	-	chr7_2:49998736-49998755	Msa1061060:intron	45.0%
!	GGGCTTTGGTGTAACATTGA+AGG	+	chr7_2:49999024-49999043	None:intergenic	45.0%
	TCTTCACAAGGGAGGATCTT+TGG	+	chr7_2:49999057-49999076	None:intergenic	45.0%
	CTGCCTAGACTTCTTCACAA+GGG	+	chr7_2:49999068-49999087	None:intergenic	45.0%
	TCTGCCTAGACTTCTTCACA+AGG	+	chr7_2:49999069-49999088	None:intergenic	45.0%
	TCAATTGCATCCTTCTGTGG+TGG	-	chr7_2:49997294-49997313	Msa1061060:intron	45.0%
	TGAGAAGTTTCGATCGGAAG+AGG	+	chr7_2:49997363-49997382	None:intergenic	45.0%
!	GCATGATCAAATTCTGAGGG+AGG	+	chr7_2:49998250-49998269	None:intergenic	45.0%
	TCCAAAGACAGCCAATTCCA+TGG	+	chr7_2:49998509-49998528	None:intergenic	45.0%
!	CTTCATCGAGAGCGAGTTTT+TGG	-	chr7_2:49998736-49998755	Msa1061060:intron	45.0%
!	GGGCTTTGGTGTAACATTGA+AGG	+	chr7_2:49999024-49999043	None:intergenic	45.0%
	TCTTCACAAGGGAGGATCTT+TGG	+	chr7_2:49999057-49999076	None:intergenic	45.0%
	CTGCCTAGACTTCTTCACAA+GGG	+	chr7_2:49999068-49999087	None:intergenic	45.0%
	TCTGCCTAGACTTCTTCACA+AGG	+	chr7_2:49999069-49999088	None:intergenic	45.0%
!!!	AATTAAATATTTTTGTTATT+TGG	-	chr7_2:49997759-49997778	Msa1061060:intron	5.0%
!!!	AATTAAATATTTTTGTTATT+TGG	-	chr7_2:49997759-49997778	Msa1061060:intron	5.0%
!!	TCGGAGATGGTGGAAGAAGA+GGG	+	chr7_2:49997145-49997164	None:intergenic	50.0%
!!	ATCGGAGATGGTGGAAGAAG+AGG	+	chr7_2:49997146-49997165	None:intergenic	50.0%
	ACAGAGGAGGATACGTCGAT+CGG	+	chr7_2:49997164-49997183	None:intergenic	50.0%
!	GGATTGACGGTGGGTTTGAT+CGG	+	chr7_2:49997221-49997240	None:intergenic	50.0%
	ATCCACCGGAGAAGGATTGA+CGG	+	chr7_2:49997234-49997253	None:intergenic	50.0%
	TGATCGATCGCATCCTTCTC+TGG	-	chr7_2:49997257-49997276	Msa1061060:intron	50.0%
	GATTGGAGATCCACCAGAGA+AGG	+	chr7_2:49997273-49997292	None:intergenic	50.0%
	GATCAGAGATCCACCACAGA+AGG	+	chr7_2:49997307-49997326	None:intergenic	50.0%
	GATCTCTGATCGATCTGCAC+CGG	-	chr7_2:49997316-49997335	Msa1061060:three_prime_UTR	50.0%
	TGAATTGCGATCGGAGACTC+AGG	+	chr7_2:49997429-49997448	None:intergenic	50.0%
	GAACATTCGAGGTGGAAGAG+TGG	-	chr7_2:49998204-49998223	Msa1061060:intron	50.0%
	GCCATGGAATTGGCTGTCTT+TGG	-	chr7_2:49998505-49998524	Msa1061060:intron	50.0%
!!!	GAGCGAGTTTTTGGTAGAGC+AGG	-	chr7_2:49998745-49998764	Msa1061060:intron	50.0%
!!	AGGGAGGATCTTTGGAGTGT+GGG	+	chr7_2:49999049-49999068	None:intergenic	50.0%
!!	AAGGGAGGATCTTTGGAGTG+TGG	+	chr7_2:49999050-49999069	None:intergenic	50.0%
	CCTAGACTTCTTCACAAGGG+AGG	+	chr7_2:49999065-49999084	None:intergenic	50.0%
	CCTCCCTTGTGAAGAAGTCT+AGG	-	chr7_2:49999062-49999081	Msa1061060:intron	50.0%
	CTGAACCCATGCTACGTAAC+TGG	-	chr7_2:49999225-49999244	Msa1061060:CDS	50.0%
!!	TCGGAGATGGTGGAAGAAGA+GGG	+	chr7_2:49997145-49997164	None:intergenic	50.0%
!!	ATCGGAGATGGTGGAAGAAG+AGG	+	chr7_2:49997146-49997165	None:intergenic	50.0%
	ACAGAGGAGGATACGTCGAT+CGG	+	chr7_2:49997164-49997183	None:intergenic	50.0%
!	GGATTGACGGTGGGTTTGAT+CGG	+	chr7_2:49997221-49997240	None:intergenic	50.0%
	ATCCACCGGAGAAGGATTGA+CGG	+	chr7_2:49997234-49997253	None:intergenic	50.0%
	TGATCGATCGCATCCTTCTC+TGG	-	chr7_2:49997257-49997276	Msa1061060:intron	50.0%
	GATTGGAGATCCACCAGAGA+AGG	+	chr7_2:49997273-49997292	None:intergenic	50.0%
	GATCAGAGATCCACCACAGA+AGG	+	chr7_2:49997307-49997326	None:intergenic	50.0%
	GATCTCTGATCGATCTGCAC+CGG	-	chr7_2:49997316-49997335	Msa1061060:three_prime_UTR	50.0%
	TGAATTGCGATCGGAGACTC+AGG	+	chr7_2:49997429-49997448	None:intergenic	50.0%
	GAACATTCGAGGTGGAAGAG+TGG	-	chr7_2:49998204-49998223	Msa1061060:intron	50.0%
	GCCATGGAATTGGCTGTCTT+TGG	-	chr7_2:49998505-49998524	Msa1061060:intron	50.0%
!!!	GAGCGAGTTTTTGGTAGAGC+AGG	-	chr7_2:49998745-49998764	Msa1061060:intron	50.0%
!!	AGGGAGGATCTTTGGAGTGT+GGG	+	chr7_2:49999049-49999068	None:intergenic	50.0%
!!	AAGGGAGGATCTTTGGAGTG+TGG	+	chr7_2:49999050-49999069	None:intergenic	50.0%
	CCTAGACTTCTTCACAAGGG+AGG	+	chr7_2:49999065-49999084	None:intergenic	50.0%
	CCTCCCTTGTGAAGAAGTCT+AGG	-	chr7_2:49999062-49999081	Msa1061060:intron	50.0%
	CTGAACCCATGCTACGTAAC+TGG	-	chr7_2:49999225-49999244	Msa1061060:CDS	50.0%
	GATACGTCGATCGGAGATGG+TGG	+	chr7_2:49997155-49997174	None:intergenic	55.0%
	GAGGATACGTCGATCGGAGA+TGG	+	chr7_2:49997158-49997177	None:intergenic	55.0%
!	TCCTCCTCTGTCTCTAGCTC+CGG	-	chr7_2:49997173-49997192	Msa1061060:three_prime_UTR	55.0%
	CGGAGGAGATAGAGATCCAC+CGG	+	chr7_2:49997195-49997214	None:intergenic	55.0%
!	ACGGTGGGTTTGATCGGAGA+CGG	+	chr7_2:49997215-49997234	None:intergenic	55.0%
	ACCCACCGTCAATCCTTCTC+CGG	-	chr7_2:49997226-49997245	Msa1061060:intron	55.0%
	ACCGGAGAAGGATTGACGGT+GGG	+	chr7_2:49997230-49997249	None:intergenic	55.0%
	GATCAGAGATCCACCGGAGA+AGG	+	chr7_2:49997242-49997261	None:intergenic	55.0%
	GCGATCGATCAGAGATCCAC+CGG	+	chr7_2:49997248-49997267	None:intergenic	55.0%
	TCGATCGCATCCTTCTCTGG+TGG	-	chr7_2:49997260-49997279	Msa1061060:intron	55.0%
	GCGATCAACCAGAGATCCAC+CGG	+	chr7_2:49997338-49997357	None:intergenic	55.0%
	CAGGAGGATGCCATCGAAGA+CGG	+	chr7_2:49997410-49997429	None:intergenic	55.0%
	ATTGCGATCGGAGACTCAGG+AGG	+	chr7_2:49997426-49997445	None:intergenic	55.0%
	CTGAGGGAGGGCTCACAATA+GGG	+	chr7_2:49998237-49998256	None:intergenic	55.0%
	TCTGAGGGAGGGCTCACAAT+AGG	+	chr7_2:49998238-49998257	None:intergenic	55.0%
!!	GGATCTTTGGAGTGTGGGCA+GGG	+	chr7_2:49999044-49999063	None:intergenic	55.0%
!!	AGGATCTTTGGAGTGTGGGC+AGG	+	chr7_2:49999045-49999064	None:intergenic	55.0%
	CTAGGCAGAAGCCTCAAGAG+CGG	-	chr7_2:49999080-49999099	Msa1061060:intron	55.0%
	AGAAGCCTCAAGAGCGGATG+AGG	-	chr7_2:49999086-49999105	Msa1061060:intron	55.0%
	GAAGCCTCAAGAGCGGATGA+GGG	-	chr7_2:49999087-49999106	Msa1061060:intron	55.0%
	AAGACCCTCATCCGCTCTTG+AGG	+	chr7_2:49999094-49999113	None:intergenic	55.0%
	AGAGCGGATGAGGGTCTTGA+CGG	-	chr7_2:49999096-49999115	Msa1061060:intron	55.0%
	GATACGTCGATCGGAGATGG+TGG	+	chr7_2:49997155-49997174	None:intergenic	55.0%
	GAGGATACGTCGATCGGAGA+TGG	+	chr7_2:49997158-49997177	None:intergenic	55.0%
!	TCCTCCTCTGTCTCTAGCTC+CGG	-	chr7_2:49997173-49997192	Msa1061060:three_prime_UTR	55.0%
	CGGAGGAGATAGAGATCCAC+CGG	+	chr7_2:49997195-49997214	None:intergenic	55.0%
!	ACGGTGGGTTTGATCGGAGA+CGG	+	chr7_2:49997215-49997234	None:intergenic	55.0%
	ACCCACCGTCAATCCTTCTC+CGG	-	chr7_2:49997226-49997245	Msa1061060:intron	55.0%
	ACCGGAGAAGGATTGACGGT+GGG	+	chr7_2:49997230-49997249	None:intergenic	55.0%
	GATCAGAGATCCACCGGAGA+AGG	+	chr7_2:49997242-49997261	None:intergenic	55.0%
	GCGATCGATCAGAGATCCAC+CGG	+	chr7_2:49997248-49997267	None:intergenic	55.0%
	TCGATCGCATCCTTCTCTGG+TGG	-	chr7_2:49997260-49997279	Msa1061060:intron	55.0%
	GCGATCAACCAGAGATCCAC+CGG	+	chr7_2:49997338-49997357	None:intergenic	55.0%
	CAGGAGGATGCCATCGAAGA+CGG	+	chr7_2:49997410-49997429	None:intergenic	55.0%
	ATTGCGATCGGAGACTCAGG+AGG	+	chr7_2:49997426-49997445	None:intergenic	55.0%
	CTGAGGGAGGGCTCACAATA+GGG	+	chr7_2:49998237-49998256	None:intergenic	55.0%
	TCTGAGGGAGGGCTCACAAT+AGG	+	chr7_2:49998238-49998257	None:intergenic	55.0%
!!	GGATCTTTGGAGTGTGGGCA+GGG	+	chr7_2:49999044-49999063	None:intergenic	55.0%
!!	AGGATCTTTGGAGTGTGGGC+AGG	+	chr7_2:49999045-49999064	None:intergenic	55.0%
	CTAGGCAGAAGCCTCAAGAG+CGG	-	chr7_2:49999080-49999099	Msa1061060:intron	55.0%
	AGAAGCCTCAAGAGCGGATG+AGG	-	chr7_2:49999086-49999105	Msa1061060:intron	55.0%
	GAAGCCTCAAGAGCGGATGA+GGG	-	chr7_2:49999087-49999106	Msa1061060:intron	55.0%
	AAGACCCTCATCCGCTCTTG+AGG	+	chr7_2:49999094-49999113	None:intergenic	55.0%
	AGAGCGGATGAGGGTCTTGA+CGG	-	chr7_2:49999096-49999115	Msa1061060:intron	55.0%
!	TGGTGGAAGAAGAGGGCGCA+CGG	+	chr7_2:49997138-49997157	None:intergenic	60.0%
	ACCGGAGCTAGAGACAGAGG+AGG	+	chr7_2:49997177-49997196	None:intergenic	60.0%
!	TCCTCTGTCTCTAGCTCCGG+TGG	-	chr7_2:49997176-49997195	Msa1061060:three_prime_UTR	60.0%
	TCCACCGGAGCTAGAGACAG+AGG	+	chr7_2:49997180-49997199	None:intergenic	60.0%
	GTGGGTTTGATCGGAGACGG+AGG	+	chr7_2:49997212-49997231	None:intergenic	60.0%
	CACCGGAGAAGGATTGACGG+TGG	+	chr7_2:49997231-49997250	None:intergenic	60.0%
	CACCGTCAATCCTTCTCCGG+TGG	-	chr7_2:49997229-49997248	Msa1061060:intron	60.0%
	CTCTGATCGATCTGCACCGG+TGG	-	chr7_2:49997319-49997338	Msa1061060:three_prime_UTR	60.0%
!	GATCTGCACCGGTGGATCTC+TGG	-	chr7_2:49997327-49997346	Msa1061060:three_prime_UTR	60.0%
	CGGCGGAGGATGGGATTGTT+CGG	+	chr7_2:49997390-49997409	None:intergenic	60.0%
	CATCGAAGACGGCGGAGGAT+GGG	+	chr7_2:49997399-49997418	None:intergenic	60.0%
	GAGGATGCCATCGAAGACGG+CGG	+	chr7_2:49997407-49997426	None:intergenic	60.0%
!	AGATGCATGGGAGCGTGCAC+AGG	-	chr7_2:49998143-49998162	Msa1061060:CDS	60.0%
	TTGGAGTGTGGGCAGGGCTT+TGG	+	chr7_2:49999038-49999057	None:intergenic	60.0%
!	TGGTGGAAGAAGAGGGCGCA+CGG	+	chr7_2:49997138-49997157	None:intergenic	60.0%
	ACCGGAGCTAGAGACAGAGG+AGG	+	chr7_2:49997177-49997196	None:intergenic	60.0%
!	TCCTCTGTCTCTAGCTCCGG+TGG	-	chr7_2:49997176-49997195	Msa1061060:three_prime_UTR	60.0%
	TCCACCGGAGCTAGAGACAG+AGG	+	chr7_2:49997180-49997199	None:intergenic	60.0%
	GTGGGTTTGATCGGAGACGG+AGG	+	chr7_2:49997212-49997231	None:intergenic	60.0%
	CACCGGAGAAGGATTGACGG+TGG	+	chr7_2:49997231-49997250	None:intergenic	60.0%
	CACCGTCAATCCTTCTCCGG+TGG	-	chr7_2:49997229-49997248	Msa1061060:intron	60.0%
	CTCTGATCGATCTGCACCGG+TGG	-	chr7_2:49997319-49997338	Msa1061060:three_prime_UTR	60.0%
!	GATCTGCACCGGTGGATCTC+TGG	-	chr7_2:49997327-49997346	Msa1061060:three_prime_UTR	60.0%
	CGGCGGAGGATGGGATTGTT+CGG	+	chr7_2:49997390-49997409	None:intergenic	60.0%
	CATCGAAGACGGCGGAGGAT+GGG	+	chr7_2:49997399-49997418	None:intergenic	60.0%
	GAGGATGCCATCGAAGACGG+CGG	+	chr7_2:49997407-49997426	None:intergenic	60.0%
!	AGATGCATGGGAGCGTGCAC+AGG	-	chr7_2:49998143-49998162	Msa1061060:CDS	60.0%
	TTGGAGTGTGGGCAGGGCTT+TGG	+	chr7_2:49999038-49999057	None:intergenic	60.0%
	GCGCACGGAGGAAGTGATGG+TGG	+	chr7_2:49997123-49997142	None:intergenic	65.0%
	AGGGCGCACGGAGGAAGTGA+TGG	+	chr7_2:49997126-49997145	None:intergenic	65.0%
!	TGGAAGAAGAGGGCGCACGG+AGG	+	chr7_2:49997135-49997154	None:intergenic	65.0%
	CCATCGAAGACGGCGGAGGA+TGG	+	chr7_2:49997400-49997419	None:intergenic	65.0%
	CCATCCTCCGCCGTCTTCGA+TGG	-	chr7_2:49997397-49997416	Msa1061060:three_prime_UTR	65.0%
	GATGCCATCGAAGACGGCGG+AGG	+	chr7_2:49997404-49997423	None:intergenic	65.0%
	GCGCACGGAGGAAGTGATGG+TGG	+	chr7_2:49997123-49997142	None:intergenic	65.0%
	AGGGCGCACGGAGGAAGTGA+TGG	+	chr7_2:49997126-49997145	None:intergenic	65.0%
!	TGGAAGAAGAGGGCGCACGG+AGG	+	chr7_2:49997135-49997154	None:intergenic	65.0%
	CCATCGAAGACGGCGGAGGA+TGG	+	chr7_2:49997400-49997419	None:intergenic	65.0%
	CCATCCTCCGCCGTCTTCGA+TGG	-	chr7_2:49997397-49997416	Msa1061060:three_prime_UTR	65.0%
	GATGCCATCGAAGACGGCGG+AGG	+	chr7_2:49997404-49997423	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr7_2	gene	49997074	49999344	49997074	ID=Msa1061060;Name=Msa1061060
chr7_2	mRNA	49997074	49999344	49997074	ID=Msa1061060-mRNA-1;Parent=Msa1061060;Name=Msa1061060-mRNA-1;_AED=0.17;_eAED=0.30;_QI=0\|0.75\|0.6\|1\|0.25\|0.4\|5\|587\|99
chr7_2	exon	49997074	49997218	49997074	ID=Msa1061060-mRNA-1:exon:9037;Parent=Msa1061060-mRNA-1
chr7_2	exon	49997298	49997717	49997298	ID=Msa1061060-mRNA-1:exon:9036;Parent=Msa1061060-mRNA-1
chr7_2	exon	49997853	49997912	49997853	ID=Msa1061060-mRNA-1:exon:9035;Parent=Msa1061060-mRNA-1
chr7_2	exon	49998126	49998213	49998126	ID=Msa1061060-mRNA-1:exon:9034;Parent=Msa1061060-mRNA-1
chr7_2	exon	49999171	49999344	49999171	ID=Msa1061060-mRNA-1:exon:9033;Parent=Msa1061060-mRNA-1
chr7_2	CDS	49999171	49999344	49999171	ID=Msa1061060-mRNA-1:cds;Parent=Msa1061060-mRNA-1
chr7_2	CDS	49998126	49998213	49998126	ID=Msa1061060-mRNA-1:cds;Parent=Msa1061060-mRNA-1
chr7_2	CDS	49997875	49997912	49997875	ID=Msa1061060-mRNA-1:cds;Parent=Msa1061060-mRNA-1
chr7_2	three_prime_UTR	49997853	49997874	49997853	ID=Msa1061060-mRNA-1:three_prime_utr;Parent=Msa1061060-mRNA-1
chr7_2	three_prime_UTR	49997298	49997717	49997298	ID=Msa1061060-mRNA-1:three_prime_utr;Parent=Msa1061060-mRNA-1
chr7_2	three_prime_UTR	49997074	49997218	49997074	ID=Msa1061060-mRNA-1:three_prime_utr;Parent=Msa1061060-mRNA-1

Gene Sequence	>Msa1061060 ATTAAAATATTATTTTATATACAATTTTCACAATTTCGTGTTTCTTCCACCATCACTTCCTCCGTGCGCCCTCTTCTTCCACCATCTCCGATCGACGTATCCTCCTCTGTCTCTAGCTCCGGTGGATCTCTATCTCCTCCGTCTCCGATCAAACCCACCGTCAATCCTTCTCCGAACATTCGAGGTGGAAGAGTGGTGCTGCACCCTATTGTGAGCCCTCCCTCAGAATTTGATCATGCAGAAAAGGGAGATGCATTGTATGCCATGGAATTGGCTGTCTTTGGAGAAGTTAGTAAATGA
Protein sequence	>Msa1061060 IKILFYIQFSQFRVSSTITSSVRPLLPPSPIDVSSSVSSSGGSLSPPSPIKPTVNPSPNIRGGRVVLHPIVSPPSEFDHAEKGDALYAMELAVFGEVSK*