AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene002257

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene002257.t1 MTR_2g009810 96.901 355 11 0 1 355 52 406 0.0 714
MS.gene002257.t1 MTR_2g009820 89.113 248 23 2 112 355 7 254 1.63e-149 422
MS.gene002257.t1 MTR_5g031190 53.295 349 161 2 8 355 11 358 2.07e-123 359
MS.gene002257.t1 MTR_5g031360 53.868 349 159 2 8 355 13 360 6.45e-123 358
MS.gene002257.t1 MTR_5g031230 54.023 348 154 4 11 355 14 358 2.69e-119 349
MS.gene002257.t1 MTR_5g031310 53.026 347 159 3 11 355 15 359 2.86e-118 347
MS.gene002257.t1 MTR_5g031305 53.314 347 158 3 11 355 15 359 1.15e-117 345
MS.gene002257.t1 MTR_5g031390 52.161 347 162 3 11 355 14 358 1.84e-117 344
MS.gene002257.t1 MTR_5g031210 54.046 346 157 2 11 355 14 358 3.11e-117 344
MS.gene002257.t1 MTR_5g031250 52.023 346 164 2 11 355 14 358 2.26e-116 342
MS.gene002257.t1 MTR_4g115300 52.738 347 160 3 11 355 14 358 3.23e-116 341
MS.gene002257.t1 MTR_5g031180 54.624 346 155 2 11 355 14 358 7.89e-116 340
MS.gene002257.t1 MTR_5g031300 51.289 349 168 2 8 355 12 359 2.79e-115 339
MS.gene002257.t1 MTR_8g068190 54.046 346 157 2 11 355 14 358 2.52e-114 336
MS.gene002257.t1 MTR_8g092630 55.072 345 152 3 11 354 71 413 4.33e-113 335
MS.gene002257.t1 MTR_5g031430 51.149 348 164 3 11 355 14 358 6.96e-111 327
MS.gene002257.t1 MTR_1g025950 52.187 343 161 3 13 353 15 356 4.30e-109 323
MS.gene002257.t1 MTR_1g107425 44.789 355 193 3 1 353 1 354 4.53e-100 300
MS.gene002257.t1 MTR_1g107425 45.455 297 159 3 1 295 1 296 1.12e-79 246
MS.gene002257.t1 MTR_5g031250 51.240 242 113 2 11 248 14 254 5.29e-74 230
MS.gene002257.t1 MTR_5g031290 55.357 112 49 1 245 355 80 191 1.07e-30 115
MS.gene002257.t1 MTR_5g031290 51.429 70 33 1 8 76 11 80 3.12e-17 79.3
MS.gene002257.t1 MTR_8g095220 24.062 320 209 8 16 305 60 375 2.66e-17 82.8
MS.gene002257.t1 MTR_8g095220 25.911 247 161 5 16 242 60 304 3.10e-16 79.0
MS.gene002257.t1 MTR_3g089940 23.919 347 229 10 36 351 37 379 2.33e-13 70.9
MS.gene002257.t1 MTR_3g089940 23.919 347 229 10 36 351 37 379 2.35e-13 70.9
MS.gene002257.t1 MTR_3g089940 24.573 293 195 7 36 304 37 327 3.43e-13 70.1
MS.gene002257.t1 MTR_0001s0660 26.280 293 190 9 34 304 34 322 3.75e-13 70.5
MS.gene002257.t1 MTR_3g089940 24.573 293 195 7 36 304 37 327 3.84e-13 70.1
MS.gene002257.t1 MTR_3g089977 23.973 292 198 7 36 304 37 327 8.04e-13 69.3
MS.gene002257.t1 MTR_3g089970 23.438 384 257 11 1 351 1 380 2.53e-12 67.8
MS.gene002257.t1 MTR_8g064610 30.102 196 107 5 57 251 128 294 8.82e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene002257.t1 AT1G72680 75.424 354 86 1 2 355 3 355 0.0 554
MS.gene002257.t1 AT4G37970 49.856 347 172 2 9 354 13 358 3.52e-113 333
MS.gene002257.t1 AT2G21890 52.586 348 158 4 11 354 8 352 7.80e-113 333
MS.gene002257.t1 AT4G39330 51.594 345 165 2 11 354 14 357 1.19e-112 332
MS.gene002257.t1 AT2G21730 52.450 347 161 4 11 354 8 353 1.22e-111 330
MS.gene002257.t1 AT4G37980 48.997 349 176 2 8 355 7 354 2.55e-103 308
MS.gene002257.t1 AT4G37990 47.851 349 180 2 8 355 7 354 6.22e-98 295
MS.gene002257.t1 AT3G19450 44.789 355 193 3 1 353 1 354 8.92e-91 276
MS.gene002257.t1 AT4G39330 50.690 290 141 2 11 299 14 302 8.44e-89 270
MS.gene002257.t1 AT4G34230 45.614 342 184 2 13 353 13 353 1.01e-87 268
MS.gene002257.t1 AT4G34230 45.614 342 184 2 13 353 13 353 1.30e-87 268
MS.gene002257.t1 AT4G37970 48.936 282 142 2 9 290 13 292 3.96e-86 262
MS.gene002257.t1 AT4G37980 49.451 273 137 1 8 280 7 278 5.42e-79 244
MS.gene002257.t1 AT4G37970 50.862 232 113 1 9 240 13 243 1.71e-74 231
MS.gene002257.t1 AT4G37970 50.862 232 113 1 9 240 13 243 2.10e-74 231
MS.gene002257.t1 AT5G63620 26.506 249 157 6 16 242 62 306 5.78e-17 81.3
MS.gene002257.t1 AT5G63620 25.225 333 215 9 16 318 62 390 6.49e-17 82.0
MS.gene002257.t1 AT5G63620 25.225 333 215 9 16 318 62 390 7.40e-17 81.6
MS.gene002257.t1 AT5G43940 26.222 225 135 4 36 238 36 251 8.59e-14 72.4
MS.gene002257.t1 AT5G43940 26.222 225 135 4 36 238 48 263 8.82e-14 72.4
MS.gene002257.t1 AT1G32780 25.552 317 188 10 36 312 37 345 3.57e-13 70.5
MS.gene002257.t1 AT1G77120 25.589 297 187 9 36 304 36 326 4.01e-12 67.4

Find 107 sgRNAs with CRISPR-Local

Find 150 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTCATATGGCAGTAAAATT+TGG 0.150937 2.3:+73228690 MS.gene002257:CDS
ATTGGAATCAGTTCTATTTC+TGG 0.200827 2.3:-73229285 None:intergenic
ATCATAATCAACACCATTAA+AGG 0.217231 2.3:-73228306 None:intergenic
GGATCAGTTTACACCTTTAA+TGG 0.243928 2.3:+73228293 MS.gene002257:CDS
TTTGTTATGCTGATGTAATT+TGG 0.289646 2.3:+73227992 MS.gene002257:CDS
GAGGAGGCGCTGAGCCTTCT+TGG 0.291889 2.3:+73228765 MS.gene002257:CDS
TCTGGTGTTCTGGTCTTAGT+TGG 0.292846 2.3:+73229033 MS.gene002257:CDS
TTACCTAAGTTAAGGCTTGC+AGG 0.313929 2.3:-73229081 None:intergenic
GTAAAATTTGGGAAGGCATT+TGG 0.314204 2.3:+73228702 MS.gene002257:CDS
TCTTGAGAGGGTCGTAAATA+AGG 0.324188 2.3:+73229326 MS.gene002257:CDS
TCTGAAGATATCTGGTGTTC+TGG 0.326227 2.3:+73229023 MS.gene002257:CDS
TTGTTATGCTGATGTAATTT+GGG 0.354641 2.3:+73227993 MS.gene002257:CDS
TGTACCACCTGCAACACTTC+CGG 0.359100 2.3:-73229214 None:intergenic
ACCTTGAAACGCTGGACATT+AGG 0.363094 2.3:-73228182 None:intergenic
CTTATCAGACACGAGATTGC+TGG 0.363214 2.3:+73228140 MS.gene002257:intron
GTCATATGGCAGTAAAATTT+GGG 0.370755 2.3:+73228691 MS.gene002257:CDS
TTGGAATCAGTTCTATTTCT+GGG 0.372653 2.3:-73229284 None:intergenic
GCAGGTCCTTTGCTTTGTGC+TGG 0.376092 2.3:+73228585 MS.gene002257:CDS
GATTAGATGAAACAACAAAT+TGG 0.376372 2.3:-73228794 None:intergenic
GAAATAGAACTGATTCCAAT+TGG 0.379523 2.3:+73229288 MS.gene002257:CDS
TCTCTTGGAGTGGTTGGTCT+TGG 0.380973 2.3:+73228660 MS.gene002257:CDS
CTGGTGTTCTGGTCTTAGTT+GGG 0.395199 2.3:+73229034 MS.gene002257:CDS
GTCGACTTCTGTGCTGCAAA+TGG 0.396290 2.3:+73229255 MS.gene002257:CDS
CTTGGAGTGGTTGGTCTTGG+TGG 0.399951 2.3:+73228663 MS.gene002257:CDS
TCTTGGTGGCCTCGGTCATA+TGG 0.406264 2.3:+73228677 MS.gene002257:CDS
ATGACACTTCTGAAGATATC+TGG 0.409523 2.3:+73229015 MS.gene002257:CDS
ATGCAACATTACCTAAGTTA+AGG 0.414851 2.3:-73229089 None:intergenic
TTTATAATTGACACGGCATC+GGG 0.416518 2.3:+73228970 MS.gene002257:CDS
TCAGGATCAAGAACTGTTGC+CGG 0.416934 2.3:+73229195 MS.gene002257:intron
GCTTCATTTGAGTACCCAAT+TGG 0.417911 2.3:-73229303 None:intergenic
TAAATAAGGACGTGAAATAT+CGG 0.419184 2.3:+73229340 MS.gene002257:CDS
TACCTGCGATTGAATTTGTA+AGG 0.426854 2.3:-73227465 None:intergenic
GGGCTGAATTTGACTTCACT+TGG 0.427549 2.3:-73229060 None:intergenic
ACTGTTGCCGGAAGTGTTGC+AGG 0.432996 2.3:+73229207 MS.gene002257:CDS
GCAGGCGTTAGCTAAATCAT+TGG 0.435251 2.3:+73228945 MS.gene002257:intron
ACAACTTACCCCGGCACAAC+AGG 0.440053 2.3:-73228045 None:intergenic
ATTTCTCTAATTCTACTTGA+GGG 0.442191 2.3:-73229385 None:intergenic
TCCTAATGTCCAGCGTTTCA+AGG 0.445977 2.3:+73228181 MS.gene002257:CDS
GTGGTTGGTCTTGGTGGCCT+CGG 0.451789 2.3:+73228669 MS.gene002257:CDS
TTCAAAGTATCCTGTTGTGC+CGG 0.454354 2.3:+73228035 MS.gene002257:CDS
AATCAACCTGGTAAGTCTCT+TGG 0.459390 2.3:+73228645 MS.gene002257:CDS
TCAAAGTATCCTGTTGTGCC+GGG 0.460698 2.3:+73228036 MS.gene002257:CDS
AGCTATCCTTTAGCTTCAGC+AGG 0.468468 2.3:+73228567 MS.gene002257:CDS
ACACGAGATTGCTGGGGTTG+TGG 0.469150 2.3:+73228148 MS.gene002257:CDS
GCTGGGGTTGTGGCAAAGGT+TGG 0.470558 2.3:+73228158 MS.gene002257:CDS
ATTCATATTATGTCGCATCA+TGG 0.471650 2.3:-73228625 None:intergenic
TTATCAGACACGAGATTGCT+GGG 0.475272 2.3:+73228141 MS.gene002257:intron
GGCATTTGGTTTGCGTGTAA+CGG 0.491251 2.3:+73228716 MS.gene002257:CDS
CGCCTTACAAATTCAATCGC+AGG 0.493938 2.3:+73227463 MS.gene002257:CDS
ATCTTCAGAAGTGTCATGTA+AGG 0.496050 2.3:-73229009 None:intergenic
AGGTGGTACAAAAGAAATAC+AGG 0.497345 2.3:+73229227 MS.gene002257:CDS
GGTAAGTCTCTTGGAGTGGT+TGG 0.503337 2.3:+73228654 MS.gene002257:CDS
TGGTGATTGCTAAGGGAGCT+CGG 0.505418 2.3:+73227931 MS.gene002257:intron
TATGATGGTACAATTACAAA+AGG 0.507275 2.3:+73228323 MS.gene002257:CDS
TGGGCAACAAGAGATGCATC+TGG 0.511542 2.3:+73227432 MS.gene002257:CDS
TTTAATGGTGTTGATTATGA+TGG 0.517784 2.3:+73228308 MS.gene002257:CDS
TCGACTTCTGTGCTGCAAAT+GGG 0.518039 2.3:+73229256 MS.gene002257:CDS
GGTGATTGCTAAGGGAGCTC+GGG 0.518767 2.3:+73227932 MS.gene002257:intron
CACGAGCATTTCAAAGAAAG+AGG 0.527313 2.3:+73228746 MS.gene002257:CDS
TTGGTGAAGATTGCCTTGCA+TGG 0.528700 2.3:+73227412 MS.gene002257:CDS
TTCAAGGTTGGTGACCATGT+TGG 0.531246 2.3:+73228197 MS.gene002257:CDS
AGTGTCATGTAAGGATCAAA+CGG 0.531817 2.3:-73229000 None:intergenic
TTCATGTACTACAATGGAAG+TGG 0.531892 2.3:-73228354 None:intergenic
AAATAGAACTGATTCCAATT+GGG 0.533719 2.3:+73229289 MS.gene002257:CDS
TATTTCTCTAATTCTACTTG+AGG 0.534812 2.3:-73229386 None:intergenic
AAAGGACCTGCTGAAGCTAA+AGG 0.536529 2.3:-73228573 None:intergenic
CATCTCTTGTTGCCCATGCA+AGG 0.536685 2.3:-73227425 None:intergenic
AATGTCCAGCGTTTCAAGGT+TGG 0.539954 2.3:+73228185 MS.gene002257:CDS
GATTGCTGGGGTTGTGGCAA+AGG 0.541441 2.3:+73228154 MS.gene002257:CDS
GGCTGAATTTGACTTCACTT+GGG 0.543028 2.3:-73229059 None:intergenic
TTCATATTATGTCGCATCAT+GGG 0.544385 2.3:-73228624 None:intergenic
TACCTAAGTTAAGGCTTGCA+GGG 0.544423 2.3:-73229080 None:intergenic
TACAAAAGAAATACAGGAGA+TGG 0.550321 2.3:+73229233 MS.gene002257:CDS
ATTAGAAGTTCATTGTGTGA+AGG 0.552235 2.3:+73228271 MS.gene002257:CDS
TTAGAAGTTCATTGTGTGAA+GGG 0.558615 2.3:+73228272 MS.gene002257:CDS
GTACTTACATCAACTCATGC+AGG 0.564363 2.3:+73228225 MS.gene002257:CDS
GTGTCATGTAAGGATCAAAC+GGG 0.564881 2.3:-73228999 None:intergenic
CGACATAATATGAATCAACC+TGG 0.568407 2.3:+73228633 MS.gene002257:CDS
AGCCCTGCAAGCCTTAACTT+AGG 0.570562 2.3:+73229078 MS.gene002257:CDS
TTACCTTTCATGTACTACAA+TGG 0.576358 2.3:-73228360 None:intergenic
CATCTAATCAAGAAGAGATG+AGG 0.579627 2.3:+73228808 MS.gene002257:CDS
CAAATTGGTCTGCACCAAGA+AGG 0.580444 2.3:-73228779 None:intergenic
CTTCCATTGTAGTACATGAA+AGG 0.582389 2.3:+73228357 MS.gene002257:CDS
TGGTGAAGATTGCCTTGCAT+GGG 0.586230 2.3:+73227413 MS.gene002257:CDS
GTGATTCCAGCACAAAGCAA+AGG 0.590358 2.3:-73228591 None:intergenic
GGTCACCAACCTTGAAACGC+TGG 0.591766 2.3:-73228190 None:intergenic
ATCTAATCAAGAAGAGATGA+GGG 0.596139 2.3:+73228809 MS.gene002257:CDS
ACTCAAATGAAGCTCTTGAG+AGG 0.606095 2.3:+73229313 MS.gene002257:CDS
TTTGGGCAAGGAATAAACAT+GGG 0.615486 2.3:+73228010 MS.gene002257:CDS
ACCTGGTAAGTCTCTTGGAG+TGG 0.618165 2.3:+73228650 MS.gene002257:CDS
TATGGCAGTAAAATTTGGGA+AGG 0.622661 2.3:+73228695 MS.gene002257:CDS
CTCAAATGAAGCTCTTGAGA+GGG 0.622942 2.3:+73229314 MS.gene002257:CDS
GATGGTACAATTACAAAAGG+AGG 0.626222 2.3:+73228326 MS.gene002257:CDS
ATTTGGGCAAGGAATAAACA+TGG 0.627500 2.3:+73228009 MS.gene002257:CDS
ATGCTGATGTAATTTGGGCA+AGG 0.629018 2.3:+73227998 MS.gene002257:CDS
TACTTACATCAACTCATGCA+GGG 0.634667 2.3:+73228226 MS.gene002257:CDS
CAAAGTATCCTGTTGTGCCG+GGG 0.639115 2.3:+73228037 MS.gene002257:CDS
GGTTGGTGACCATGTTGGAG+TGG 0.639841 2.3:+73228202 MS.gene002257:CDS
TTGGGCAAGGAATAAACATG+GGG 0.641841 2.3:+73228011 MS.gene002257:CDS
TATGTTAAAATTACGCACTG+TGG 0.646530 2.3:+73227967 MS.gene002257:CDS
GAGCATTTCAAAGAAAGAGG+AGG 0.653117 2.3:+73228749 MS.gene002257:CDS
GTTGGTGACCATGTTGGAGT+GGG 0.658549 2.3:+73228203 MS.gene002257:CDS
TGTAAGTACCCACTCCAACA+TGG 0.660381 2.3:-73228211 None:intergenic
ACCACTCCAAGAGACTTACC+AGG 0.671497 2.3:-73228651 None:intergenic
TATCAGACACGAGATTGCTG+GGG 0.696618 2.3:+73228142 MS.gene002257:intron
GCTATGAGTTCTGAAAGTGT+TGG 0.742932 2.3:+73227393 None:intergenic
GTTGCCGGAAGTGTTGCAGG+TGG 0.789484 2.3:+73229210 MS.gene002257:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCAGGATTTAATTTTTTTTT+TGG - chr2.3:73227900-73227919 None:intergenic 15.0%
!!! TTCATAACTTCTTTTATATA+TGG + chr2.3:73228508-73228527 MS.gene002257:intron 15.0%
!!! AAATGTTTCTAATGACTTAA+CGG - chr2.3:73227606-73227625 None:intergenic 20.0%
!!! ATGAGATTTTACTTAAAACT+CGG - chr2.3:73227725-73227744 None:intergenic 20.0%
!!! ATTGGATTTTATAATTGACA+CGG + chr2.3:73228963-73228982 MS.gene002257:CDS 20.0%
!!! CTTCTTTGATTTCATAATAA+TGG + chr2.3:73228470-73228489 MS.gene002257:intron 20.0%
!!! TGTTTTTTTAACTAAACTGA+TGG + chr2.3:73228912-73228931 MS.gene002257:intron 20.0%
!!! TTTTTTGGAAAAATGTAACA+TGG - chr2.3:73227885-73227904 None:intergenic 20.0%
! AAAGAAAAACACAGATAAGT+AGG - chr2.3:73228117-73228136 None:intergenic 25.0%
! AAATCCTGACAATATGATAA+TGG + chr2.3:73227911-73227930 MS.gene002257:intron 25.0%
! AATGCATACATTCTTATATG+TGG - chr2.3:73227845-73227864 None:intergenic 25.0%
! ATATATACTGTCAGTGTAAA+GGG + chr2.3:73227554-73227573 MS.gene002257:intron 25.0%
! ATCATAATCAACACCATTAA+AGG - chr2.3:73228309-73228328 None:intergenic 25.0%
! ATGCATACATTCTTATATGT+GGG - chr2.3:73227844-73227863 None:intergenic 25.0%
! GATTAGATGAAACAACAAAT+TGG - chr2.3:73228797-73228816 None:intergenic 25.0%
! TAAATAAGGACGTGAAATAT+CGG + chr2.3:73229340-73229359 MS.gene002257:CDS 25.0%
! TAATAAATTTCAGCAAGAGA+AGG - chr2.3:73228435-73228454 None:intergenic 25.0%
! TATATATACTGTCAGTGTAA+AGG + chr2.3:73227553-73227572 MS.gene002257:intron 25.0%
! TATGATGGTACAATTACAAA+AGG + chr2.3:73228323-73228342 MS.gene002257:CDS 25.0%
! TGAAAGGTAAACTTAATAGA+TGG + chr2.3:73228373-73228392 MS.gene002257:intron 25.0%
! TTGTTATGCTGATGTAATTT+GGG + chr2.3:73227993-73228012 MS.gene002257:CDS 25.0%
!! AAATAGAACTGATTCCAATT+GGG + chr2.3:73229289-73229308 MS.gene002257:CDS 25.0%
!! CTTTGATTTCATAATAATGG+AGG + chr2.3:73228473-73228492 MS.gene002257:intron 25.0%
!! TTGAATGGAGTAATTCTTTT+AGG + chr2.3:73227518-73227537 MS.gene002257:intron 25.0%
!! TTTGTTATGCTGATGTAATT+TGG + chr2.3:73227992-73228011 MS.gene002257:CDS 25.0%
!!! TTTAATGGTGTTGATTATGA+TGG + chr2.3:73228308-73228327 MS.gene002257:CDS 25.0%
AGAAAATGCTATGGTATAGA+TGG - chr2.3:73228850-73228869 None:intergenic 30.0%
ATCACCATTATCATATTGTC+AGG - chr2.3:73227918-73227937 None:intergenic 30.0%
ATCTAATCAAGAAGAGATGA+GGG + chr2.3:73228809-73228828 MS.gene002257:CDS 30.0%
ATGCAACATTACCTAAGTTA+AGG - chr2.3:73229092-73229111 None:intergenic 30.0%
ATGGTTTGAAGAAAATGCTA+TGG - chr2.3:73228859-73228878 None:intergenic 30.0%
ATTAGAAGTTCATTGTGTGA+AGG + chr2.3:73228271-73228290 MS.gene002257:CDS 30.0%
ATTCATATTATGTCGCATCA+TGG - chr2.3:73228628-73228647 None:intergenic 30.0%
ATTGGAATCAGTTCTATTTC+TGG - chr2.3:73229288-73229307 None:intergenic 30.0%
GTCATATGGCAGTAAAATTT+GGG + chr2.3:73228691-73228710 MS.gene002257:CDS 30.0%
TACAAAAGAAATACAGGAGA+TGG + chr2.3:73229233-73229252 MS.gene002257:CDS 30.0%
TATGTTAAAATTACGCACTG+TGG + chr2.3:73227967-73227986 MS.gene002257:CDS 30.0%
TGAATGAAGGATATTACTTC+TGG - chr2.3:73229168-73229187 None:intergenic 30.0%
TTACCTTTCATGTACTACAA+TGG - chr2.3:73228363-73228382 None:intergenic 30.0%
TTAGAAGTTCATTGTGTGAA+GGG + chr2.3:73228272-73228291 MS.gene002257:CDS 30.0%
TTCATATTATGTCGCATCAT+GGG - chr2.3:73228627-73228646 None:intergenic 30.0%
! GAAATAGAACTGATTCCAAT+TGG + chr2.3:73229288-73229307 MS.gene002257:CDS 30.0%
! TTGGAATCAGTTCTATTTCT+GGG - chr2.3:73229287-73229306 None:intergenic 30.0%
! TTTTATAATTGACACGGCAT+CGG + chr2.3:73228969-73228988 MS.gene002257:CDS 30.0%
!! ATGATAATGGTGATTGCTAA+GGG + chr2.3:73227924-73227943 MS.gene002257:intron 30.0%
!! CTCATTTTCCTTTTGTTTCA+AGG + chr2.3:73227740-73227759 MS.gene002257:intron 30.0%
!! TATGATAATGGTGATTGCTA+AGG + chr2.3:73227923-73227942 MS.gene002257:intron 30.0%
AGGTGGTACAAAAGAAATAC+AGG + chr2.3:73229227-73229246 MS.gene002257:CDS 35.0%
AGTGTCATGTAAGGATCAAA+CGG - chr2.3:73229003-73229022 None:intergenic 35.0%
ATTTGGGCAAGGAATAAACA+TGG + chr2.3:73228009-73228028 MS.gene002257:CDS 35.0%
CAGTATCAGTATTCTCTTCA+TGG - chr2.3:73228878-73228897 None:intergenic 35.0%
CATCTAATCAAGAAGAGATG+AGG + chr2.3:73228808-73228827 MS.gene002257:CDS 35.0%
CGACATAATATGAATCAACC+TGG + chr2.3:73228633-73228652 MS.gene002257:CDS 35.0%
CTTCCATTGTAGTACATGAA+AGG + chr2.3:73228357-73228376 MS.gene002257:CDS 35.0%
GATGGTACAATTACAAAAGG+AGG + chr2.3:73228326-73228345 MS.gene002257:CDS 35.0%
GGATCAGTTTACACCTTTAA+TGG + chr2.3:73228293-73228312 MS.gene002257:CDS 35.0%
GGTAAACTTAATAGATGGCT+TGG + chr2.3:73228378-73228397 MS.gene002257:intron 35.0%
GGTCATATGGCAGTAAAATT+TGG + chr2.3:73228690-73228709 MS.gene002257:CDS 35.0%
TACCTGCGATTGAATTTGTA+AGG - chr2.3:73227468-73227487 None:intergenic 35.0%
TACTTACATCAACTCATGCA+GGG + chr2.3:73228226-73228245 MS.gene002257:CDS 35.0%
TATGGCAGTAAAATTTGGGA+AGG + chr2.3:73228695-73228714 MS.gene002257:CDS 35.0%
TCCTGAAAACAGATGAATGA+AGG - chr2.3:73229181-73229200 None:intergenic 35.0%
TTCATGTACTACAATGGAAG+TGG - chr2.3:73228357-73228376 None:intergenic 35.0%
TTTATAATTGACACGGCATC+GGG + chr2.3:73228970-73228989 MS.gene002257:CDS 35.0%
TTTGGGCAAGGAATAAACAT+GGG + chr2.3:73228010-73228029 MS.gene002257:CDS 35.0%
! ATATATGGTCTTGCATATGC+AGG + chr2.3:73228523-73228542 MS.gene002257:intron 35.0%
! ATGACACTTCTGAAGATATC+TGG + chr2.3:73229015-73229034 MS.gene002257:CDS 35.0%
! CTGCAATAACCAGTAACTTT+TGG - chr2.3:73227777-73227796 None:intergenic 35.0%
! GAAGCTAAAGGATAGCTTTT+AGG - chr2.3:73228564-73228583 None:intergenic 35.0%
! TCCTTCATTCATCTGTTTTC+AGG + chr2.3:73229177-73229196 MS.gene002257:intron 35.0%
! TGAAAGTGTAGCATCAAACT+AGG - chr2.3:73227802-73227821 None:intergenic 35.0%
! TGCATTTATGTATGACTTGC+TGG + chr2.3:73227859-73227878 MS.gene002257:intron 35.0%
! TTTAAAGTCACACACATGCT+TGG + chr2.3:73227645-73227664 MS.gene002257:intron 35.0%
!! ATCTTCAGAAGTGTCATGTA+AGG - chr2.3:73229012-73229031 None:intergenic 35.0%
!! GTAAAATTTGGGAAGGCATT+TGG + chr2.3:73228702-73228721 MS.gene002257:CDS 35.0%
AATCAACCTGGTAAGTCTCT+TGG + chr2.3:73228645-73228664 MS.gene002257:CDS 40.0%
ACCTTGCTACCAAAAGTTAC+TGG + chr2.3:73227765-73227784 MS.gene002257:intron 40.0%
ACTCAAATGAAGCTCTTGAG+AGG + chr2.3:73229313-73229332 MS.gene002257:CDS 40.0%
ATGCTGATGTAATTTGGGCA+AGG + chr2.3:73227998-73228017 MS.gene002257:CDS 40.0%
ATTCAACAAAGCAGAGTGAG+AGG - chr2.3:73227504-73227523 None:intergenic 40.0%
CAACTCGTTCTTTATATCGC+TGG + chr2.3:73229140-73229159 MS.gene002257:intron 40.0%
CAAGGTTGCCTTGAAACAAA+AGG - chr2.3:73227751-73227770 None:intergenic 40.0%
CACGAGCATTTCAAAGAAAG+AGG + chr2.3:73228746-73228765 MS.gene002257:CDS 40.0%
CTCAAATGAAGCTCTTGAGA+GGG + chr2.3:73229314-73229333 MS.gene002257:CDS 40.0%
GAGCATTTCAAAGAAAGAGG+AGG + chr2.3:73228749-73228768 MS.gene002257:CDS 40.0%
GCTTCATTTGAGTACCCAAT+TGG - chr2.3:73229306-73229325 None:intergenic 40.0%
GTACTTACATCAACTCATGC+AGG + chr2.3:73228225-73228244 MS.gene002257:CDS 40.0%
GTGTCATGTAAGGATCAAAC+GGG - chr2.3:73229002-73229021 None:intergenic 40.0%
TACCTAAGTTAAGGCTTGCA+GGG - chr2.3:73229083-73229102 None:intergenic 40.0%
TCTGAAGATATCTGGTGTTC+TGG + chr2.3:73229023-73229042 MS.gene002257:CDS 40.0%
TCTTGAGAGGGTCGTAAATA+AGG + chr2.3:73229326-73229345 MS.gene002257:CDS 40.0%
TTACCTAAGTTAAGGCTTGC+AGG - chr2.3:73229084-73229103 None:intergenic 40.0%
TTATCAGACACGAGATTGCT+GGG + chr2.3:73228141-73228160 MS.gene002257:intron 40.0%
TTGGGCAAGGAATAAACATG+GGG + chr2.3:73228011-73228030 MS.gene002257:CDS 40.0%
! AGCTATTCTTGCTGTGTGTA+TGG + chr2.3:73228069-73228088 MS.gene002257:intron 40.0%
! ATTTTACTGCCATATGACCG+AGG - chr2.3:73228689-73228708 None:intergenic 40.0%
! GGCTGAATTTGACTTCACTT+GGG - chr2.3:73229062-73229081 None:intergenic 40.0%
! TCTCACTCTGCTTTGTTGAA+TGG + chr2.3:73227503-73227522 MS.gene002257:intron 40.0%
! TTCAAAGTATCCTGTTGTGC+CGG + chr2.3:73228035-73228054 MS.gene002257:CDS 40.0%
!! ACCAGTAACTTTTGGTAGCA+AGG - chr2.3:73227769-73227788 None:intergenic 40.0%
AAAGGACCTGCTGAAGCTAA+AGG - chr2.3:73228576-73228595 None:intergenic 45.0%
AATGTCCAGCGTTTCAAGGT+TGG + chr2.3:73228185-73228204 MS.gene002257:CDS 45.0%
ACCTTGAAACGCTGGACATT+AGG - chr2.3:73228185-73228204 None:intergenic 45.0%
CGCCTTACAAATTCAATCGC+AGG + chr2.3:73227463-73227482 MS.gene002257:CDS 45.0%
CTTATCAGACACGAGATTGC+TGG + chr2.3:73228140-73228159 MS.gene002257:intron 45.0%
GAATAGCTCACAACTTACCC+CGG - chr2.3:73228057-73228076 None:intergenic 45.0%
GCAGGCGTTAGCTAAATCAT+TGG + chr2.3:73228945-73228964 MS.gene002257:intron 45.0%
GTGATTCCAGCACAAAGCAA+AGG - chr2.3:73228594-73228613 None:intergenic 45.0%
TATCAGACACGAGATTGCTG+GGG + chr2.3:73228142-73228161 MS.gene002257:intron 45.0%
TCAGGATCAAGAACTGTTGC+CGG + chr2.3:73229195-73229214 MS.gene002257:intron 45.0%
TCCTAATGTCCAGCGTTTCA+AGG + chr2.3:73228181-73228200 MS.gene002257:CDS 45.0%
TCGACTTCTGTGCTGCAAAT+GGG + chr2.3:73229256-73229275 MS.gene002257:CDS 45.0%
TGGTGAAGATTGCCTTGCAT+GGG + chr2.3:73227413-73227432 MS.gene002257:CDS 45.0%
TGTAAGTACCCACTCCAACA+TGG - chr2.3:73228214-73228233 None:intergenic 45.0%
TTGGTGAAGATTGCCTTGCA+TGG + chr2.3:73227412-73227431 MS.gene002257:CDS 45.0%
! ACTGATGGTGACTTCTATGC+AGG + chr2.3:73228927-73228946 MS.gene002257:intron 45.0%
! AGCTATCCTTTAGCTTCAGC+AGG + chr2.3:73228567-73228586 MS.gene002257:CDS 45.0%
! CAAATTGGTCTGCACCAAGA+AGG - chr2.3:73228782-73228801 None:intergenic 45.0%
! GGGCTGAATTTGACTTCACT+TGG - chr2.3:73229063-73229082 None:intergenic 45.0%
! TCAAAGTATCCTGTTGTGCC+GGG + chr2.3:73228036-73228055 MS.gene002257:CDS 45.0%
!! CTGGTGTTCTGGTCTTAGTT+GGG + chr2.3:73229034-73229053 MS.gene002257:CDS 45.0%
!! GGCATTTGGTTTGCGTGTAA+CGG + chr2.3:73228716-73228735 MS.gene002257:CDS 45.0%
!! TCTGGTGTTCTGGTCTTAGT+TGG + chr2.3:73229033-73229052 MS.gene002257:CDS 45.0%
!! TTCAAGGTTGGTGACCATGT+TGG + chr2.3:73228197-73228216 MS.gene002257:CDS 45.0%
ACCACTCCAAGAGACTTACC+AGG - chr2.3:73228654-73228673 None:intergenic 50.0%
ACCTGGTAAGTCTCTTGGAG+TGG + chr2.3:73228650-73228669 MS.gene002257:CDS 50.0%
AGCCCTGCAAGCCTTAACTT+AGG + chr2.3:73229078-73229097 MS.gene002257:CDS 50.0%
CATCTCTTGTTGCCCATGCA+AGG - chr2.3:73227428-73227447 None:intergenic 50.0%
GGTAAGTCTCTTGGAGTGGT+TGG + chr2.3:73228654-73228673 MS.gene002257:CDS 50.0%
TGGGCAACAAGAGATGCATC+TGG + chr2.3:73227432-73227451 MS.gene002257:CDS 50.0%
TGGTGATTGCTAAGGGAGCT+CGG + chr2.3:73227931-73227950 MS.gene002257:intron 50.0%
TGTACCACCTGCAACACTTC+CGG - chr2.3:73229217-73229236 None:intergenic 50.0%
! CAAAGTATCCTGTTGTGCCG+GGG + chr2.3:73228037-73228056 MS.gene002257:CDS 50.0%
! GTCGACTTCTGTGCTGCAAA+TGG + chr2.3:73229255-73229274 MS.gene002257:CDS 50.0%
!! GTTGGTGACCATGTTGGAGT+GGG + chr2.3:73228203-73228222 MS.gene002257:CDS 50.0%
!! TCTCTTGGAGTGGTTGGTCT+TGG + chr2.3:73228660-73228679 MS.gene002257:CDS 50.0%
ACAACTTACCCCGGCACAAC+AGG - chr2.3:73228048-73228067 None:intergenic 55.0%
ACACGAGATTGCTGGGGTTG+TGG + chr2.3:73228148-73228167 MS.gene002257:CDS 55.0%
GATTGCTGGGGTTGTGGCAA+AGG + chr2.3:73228154-73228173 MS.gene002257:CDS 55.0%
GCAGGTCCTTTGCTTTGTGC+TGG + chr2.3:73228585-73228604 MS.gene002257:CDS 55.0%
GGTCACCAACCTTGAAACGC+TGG - chr2.3:73228193-73228212 None:intergenic 55.0%
GGTGATTGCTAAGGGAGCTC+GGG + chr2.3:73227932-73227951 MS.gene002257:intron 55.0%
! TCTTGGTGGCCTCGGTCATA+TGG + chr2.3:73228677-73228696 MS.gene002257:CDS 55.0%
!! ACTGTTGCCGGAAGTGTTGC+AGG + chr2.3:73229207-73229226 MS.gene002257:CDS 55.0%
!! CTTGGAGTGGTTGGTCTTGG+TGG + chr2.3:73228663-73228682 MS.gene002257:CDS 55.0%
!! GGTTGGTGACCATGTTGGAG+TGG + chr2.3:73228202-73228221 MS.gene002257:CDS 55.0%
GCTGGGGTTGTGGCAAAGGT+TGG + chr2.3:73228158-73228177 MS.gene002257:CDS 60.0%
!! GTGGTTGGTCTTGGTGGCCT+CGG + chr2.3:73228669-73228688 MS.gene002257:CDS 60.0%
!! GTTGCCGGAAGTGTTGCAGG+TGG + chr2.3:73229210-73229229 MS.gene002257:CDS 60.0%
!! GAGGAGGCGCTGAGCCTTCT+TGG + chr2.3:73228765-73228784 MS.gene002257:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 73227396 73229395 73227396 ID=MS.gene002257
chr2.3 mRNA 73227396 73229395 73227396 ID=MS.gene002257.t1;Parent=MS.gene002257
chr2.3 exon 73227396 73227484 73227396 ID=MS.gene002257.t1.exon1;Parent=MS.gene002257.t1
chr2.3 CDS 73227396 73227484 73227396 ID=cds.MS.gene002257.t1;Parent=MS.gene002257.t1
chr2.3 exon 73227945 73228058 73227945 ID=MS.gene002257.t1.exon2;Parent=MS.gene002257.t1
chr2.3 CDS 73227945 73228058 73227945 ID=cds.MS.gene002257.t1;Parent=MS.gene002257.t1
chr2.3 exon 73228148 73228378 73228148 ID=MS.gene002257.t1.exon3;Parent=MS.gene002257.t1
chr2.3 CDS 73228148 73228378 73228148 ID=cds.MS.gene002257.t1;Parent=MS.gene002257.t1
chr2.3 exon 73228545 73228830 73228545 ID=MS.gene002257.t1.exon4;Parent=MS.gene002257.t1
chr2.3 CDS 73228545 73228830 73228545 ID=cds.MS.gene002257.t1;Parent=MS.gene002257.t1
chr2.3 exon 73228949 73229099 73228949 ID=MS.gene002257.t1.exon5;Parent=MS.gene002257.t1
chr2.3 CDS 73228949 73229099 73228949 ID=cds.MS.gene002257.t1;Parent=MS.gene002257.t1
chr2.3 exon 73229199 73229395 73229199 ID=MS.gene002257.t1.exon6;Parent=MS.gene002257.t1
chr2.3 CDS 73229199 73229395 73229199 ID=cds.MS.gene002257.t1;Parent=MS.gene002257.t1
Gene Sequence

>MS.gene002257

ATGAGTTCTGAAAGTGTTGGTGAAGATTGCCTTGCATGGGCAACAAGAGATGCATCTGGAGTTCTGTCGCCTTACAAATTCAATCGCAGGTATATTTGTTATGTACCTCTCACTCTGCTTTGTTGAATGGAGTAATTCTTTTAGGATTTAATTTTTATATATATACTGTCAGTGTAAAGGGTTTTTAGACTTGTCACTCAATCATAACCGTTAAGTCATTAGAAACATTTGACTTTTATCATAATCGCTTTTAAAGTCACACACATGCTTGGTAGTGATGTGTTGACAGTGTAAAACTTATACAATCAGTGTATGAAAGTTAAACTCCGAGTTTTAAGTAAAATCTCATTTTCCTTTTGTTTCAAGGCAACCTTGCTACCAAAAGTTACTGGTTATTGCAGTTCCTAGTTTGATGCTACACTTTCATATCATATCAATTCAAATGCCCACATATAAGAATGTATGCATTTATGTATGACTTGCTGGCCATGTTACATTTTTCCAAAAAAAAAATTAAATCCTGACAATATGATAATGGTGATTGCTAAGGGAGCTCGGGAGTGAAGATGTTTATGTTAAAATTACGCACTGTGGCGTTTGTTATGCTGATGTAATTTGGGCAAGGAATAAACATGGGGATTCAAAGTATCCTGTTGTGCCGGGGTAAGTTGTGAGCTATTCTTGCTGTGTGTATGGTTGAGAATGTTATATACATAAGCCTACTTATCTGTGTTTTTCTTTTTTCTTATCAGACACGAGATTGCTGGGGTTGTGGCAAAGGTTGGTCCTAATGTCCAGCGTTTCAAGGTTGGTGACCATGTTGGAGTGGGTACTTACATCAACTCATGCAGGGAATGTGAGTATTGTAACGATCGATTAGAAGTTCATTGTGTGAAGGGATCAGTTTACACCTTTAATGGTGTTGATTATGATGGTACAATTACAAAAGGAGGATACTCCACTTCCATTGTAGTACATGAAAGGTAAACTTAATAGATGGCTTGGTTCTGATTATGTTTTATGTGTTTTTATGAGACCTTCTCTTGCTGAAATTTATTATAAGCACTAAAACAACTTCTTTGATTTCATAATAATGGAGGTTGTAATTTCAGTTCATAACTTCTTTTATATATGGTCTTGCATATGCAGGTATTGCTTCTTGATACCTAAAAGCTATCCTTTAGCTTCAGCAGGTCCTTTGCTTTGTGCTGGAATCACTGTTTATTCTCCCATGATGCGACATAATATGAATCAACCTGGTAAGTCTCTTGGAGTGGTTGGTCTTGGTGGCCTCGGTCATATGGCAGTAAAATTTGGGAAGGCATTTGGTTTGCGTGTAACGGTTTTCAGCACGAGCATTTCAAAGAAAGAGGAGGCGCTGAGCCTTCTTGGTGCAGACCAATTTGTTGTTTCATCTAATCAAGAAGAGATGAGGGTAAGTATTATATGAACCATCTATACCATAGCATTTTCTTCAAACCATGAAGAGAATACTGATACTGATGACATGTGCTTCTGTTTTTTTAACTAAACTGATGGTGACTTCTATGCAGGCGTTAGCTAAATCATTGGATTTTATAATTGACACGGCATCGGGTGATCACCCGTTTGATCCTTACATGACACTTCTGAAGATATCTGGTGTTCTGGTCTTAGTTGGGTTCCCAAGTGAAGTCAAATTCAGCCCTGCAAGCCTTAACTTAGGTAATGTTGCATTTGAAGTATAGAAGTTTTGTTTGAATAACAACTCGTTCTTTATATCGCTGGAACCAGAAGTAATATCCTTCATTCATCTGTTTTCAGGATCAAGAACTGTTGCCGGAAGTGTTGCAGGTGGTACAAAAGAAATACAGGAGATGGTCGACTTCTGTGCTGCAAATGGGATTCACCCAGAAATAGAACTGATTCCAATTGGGTACTCAAATGAAGCTCTTGAGAGGGTCGTAAATAAGGACGTGAAATATCGGTTTGTAATAGACATTGAAAATTCCCTCAAGTAG
Protein sequence

>MS.gene002257.t1

MSSESVGEDCLAWATRDASGVLSPYKFNRRELGSEDVYVKITHCGVCYADVIWARNKHGDSKYPVVPGHEIAGVVAKVGPNVQRFKVGDHVGVGTYINSCRECEYCNDRLEVHCVKGSVYTFNGVDYDGTITKGGYSTSIVVHERYCFLIPKSYPLASAGPLLCAGITVYSPMMRHNMNQPGKSLGVVGLGGLGHMAVKFGKAFGLRVTVFSTSISKKEEALSLLGADQFVVSSNQEEMRALAKSLDFIIDTASGDHPFDPYMTLLKISGVLVLVGFPSEVKFSPASLNLGSRTVAGSVAGGTKEIQEMVDFCAANGIHPEIELIPIGYSNEALERVVNKDVKYRFVIDIENSLK