AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene068619

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068619.t1 MTR_5g031210 96.676 361 12 0 1 361 1 361 0.0 719
MS.gene068619.t1 MTR_5g031230 93.906 361 22 0 1 361 1 361 0.0 673
MS.gene068619.t1 MTR_4g115300 83.934 361 58 0 1 361 1 361 0.0 624
MS.gene068619.t1 MTR_5g031250 83.657 361 59 0 1 361 1 361 0.0 615
MS.gene068619.t1 MTR_5g031310 80.332 361 70 1 1 360 1 361 0.0 588
MS.gene068619.t1 MTR_5g031305 80.609 361 69 1 1 360 1 361 0.0 587
MS.gene068619.t1 MTR_5g031390 79.224 361 75 0 1 361 1 361 0.0 583
MS.gene068619.t1 MTR_5g031300 79.006 362 75 1 1 361 1 362 0.0 582
MS.gene068619.t1 MTR_5g031360 76.638 351 82 0 10 360 12 362 0.0 557
MS.gene068619.t1 MTR_5g031190 73.961 361 94 0 1 361 1 361 0.0 551
MS.gene068619.t1 MTR_8g068190 76.389 360 85 0 1 360 1 360 0.0 545
MS.gene068619.t1 MTR_5g031180 77.008 361 83 0 1 361 1 361 0.0 542
MS.gene068619.t1 MTR_8g092630 62.994 354 130 1 4 357 61 413 4.02e-151 432
MS.gene068619.t1 MTR_5g031250 84.647 241 37 0 1 241 1 241 4.67e-145 411
MS.gene068619.t1 MTR_5g031430 60.000 345 138 0 14 358 14 358 7.56e-141 404
MS.gene068619.t1 MTR_1g025950 60.345 348 137 1 9 355 8 355 1.78e-138 398
MS.gene068619.t1 MTR_2g009810 54.624 346 155 2 14 358 62 406 1.38e-118 349
MS.gene068619.t1 MTR_1g107425 53.097 339 159 0 15 353 13 351 3.39e-118 346
MS.gene068619.t1 MTR_1g107425 53.405 279 129 1 15 293 13 290 2.82e-93 281
MS.gene068619.t1 MTR_2g009820 50.413 242 111 2 123 358 16 254 5.08e-65 207
MS.gene068619.t1 MTR_5g031290 74.561 114 29 0 247 360 80 193 3.74e-57 184
MS.gene068619.t1 MTR_5g031290 86.250 80 10 1 1 79 1 80 5.18e-44 150
MS.gene068619.t1 MTR_8g095220 27.333 300 177 10 7 279 59 344 1.05e-20 93.2
MS.gene068619.t1 MTR_8g095220 28.736 261 150 9 7 245 59 305 3.52e-20 90.1
MS.gene068619.t1 MTR_0001s0660 27.393 303 194 9 38 318 35 333 2.64e-19 89.4
MS.gene068619.t1 MTR_6g466320 26.176 340 222 11 1 325 6 331 7.18e-17 81.3
MS.gene068619.t1 MTR_6g465010 26.176 340 222 11 1 325 6 331 7.18e-17 81.3
MS.gene068619.t1 MTR_3g089977 23.782 349 227 11 39 354 37 379 1.42e-16 80.5
MS.gene068619.t1 MTR_3g089940 24.189 339 219 10 39 344 37 370 1.44e-16 80.5
MS.gene068619.t1 MTR_3g089940 24.189 339 219 10 39 344 37 370 1.61e-16 80.5
MS.gene068619.t1 MTR_3g089970 23.894 339 220 10 39 344 38 371 3.00e-16 79.7
MS.gene068619.t1 MTR_6g465010 26.582 316 204 10 25 325 13 315 4.33e-16 79.0
MS.gene068619.t1 MTR_3g089940 24.430 307 202 8 39 319 37 339 1.56e-14 74.3
MS.gene068619.t1 MTR_3g089940 24.430 307 202 8 39 319 37 339 1.73e-14 74.3
MS.gene068619.t1 MTR_5g045710 26.316 209 139 3 38 233 35 241 1.12e-13 72.0
MS.gene068619.t1 MTR_7g068320 26.648 349 219 14 39 353 48 393 4.43e-12 67.0
MS.gene068619.t1 MTR_5g031380 62.500 40 15 0 81 120 20 59 1.50e-11 61.2
MS.gene068619.t1 MTR_3g099460 32.877 146 80 4 39 167 37 181 3.82e-11 64.3
MS.gene068619.t1 MTR_3g099460 32.877 146 80 4 39 167 44 188 4.29e-11 64.3
MS.gene068619.t1 MTR_3g099460 33.103 145 79 4 39 166 37 180 6.45e-11 63.2
MS.gene068619.t1 MTR_3g099460 33.103 145 79 4 39 166 44 187 7.17e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068619.t1 AT4G37980 69.714 350 106 0 11 360 7 356 1.10e-173 487
MS.gene068619.t1 AT4G37990 68.857 350 109 0 11 360 7 356 4.18e-167 471
MS.gene068619.t1 AT4G39330 61.560 359 138 0 1 359 1 359 5.57e-165 465
MS.gene068619.t1 AT4G37970 62.817 355 132 0 6 360 7 361 2.74e-164 464
MS.gene068619.t1 AT2G21890 61.080 352 136 1 11 361 5 356 1.60e-155 442
MS.gene068619.t1 AT2G21730 61.190 353 135 2 11 361 5 357 2.29e-154 439
MS.gene068619.t1 AT4G37980 70.035 287 86 0 11 297 7 293 2.87e-136 390
MS.gene068619.t1 AT4G39330 61.794 301 115 0 1 301 1 301 6.23e-134 385
MS.gene068619.t1 AT4G37970 62.329 292 110 0 6 297 7 298 4.77e-128 369
MS.gene068619.t1 AT1G72680 51.429 350 165 2 11 358 9 355 1.95e-116 342
MS.gene068619.t1 AT3G19450 52.023 346 166 0 11 356 9 354 1.04e-109 325
MS.gene068619.t1 AT4G34230 52.450 347 165 0 7 353 4 350 4.30e-104 310
MS.gene068619.t1 AT4G34230 52.161 347 166 0 7 353 4 350 3.50e-103 308
MS.gene068619.t1 AT4G37970 62.447 237 89 0 6 242 7 243 4.45e-101 299
MS.gene068619.t1 AT4G37970 62.447 237 89 0 6 242 7 243 6.16e-101 299
MS.gene068619.t1 AT5G63620 25.076 331 205 11 7 308 61 377 2.11e-20 92.4
MS.gene068619.t1 AT5G63620 25.333 300 183 10 7 279 61 346 3.11e-20 91.7
MS.gene068619.t1 AT5G63620 26.744 258 153 9 7 242 61 304 6.43e-20 89.7
MS.gene068619.t1 AT1G32780 27.123 365 213 16 39 358 37 393 8.05e-17 81.6
MS.gene068619.t1 AT5G43940 27.429 350 209 12 39 352 36 376 5.48e-16 79.0
MS.gene068619.t1 AT5G43940 27.429 350 209 12 39 352 48 388 6.28e-16 79.0
MS.gene068619.t1 AT1G77120 24.348 345 230 10 39 354 36 378 1.91e-15 77.4
MS.gene068619.t1 AT5G51970 25.526 333 219 11 8 325 15 333 3.44e-15 76.3
MS.gene068619.t1 AT5G51970 25.526 333 219 11 8 325 15 333 3.44e-15 76.3
MS.gene068619.t1 AT1G22430 25.503 298 189 9 39 307 42 335 8.93e-15 75.5
MS.gene068619.t1 AT1G22430 25.503 298 189 9 39 307 42 335 8.93e-15 75.5
MS.gene068619.t1 AT1G22430 25.943 212 139 4 39 233 42 252 1.03e-13 71.6
MS.gene068619.t1 AT1G22430 25.943 212 139 4 39 233 42 252 1.35e-13 70.9
MS.gene068619.t1 AT1G22430 25.943 212 139 4 39 233 42 252 1.35e-13 70.9
MS.gene068619.t1 AT1G22440 27.273 187 118 4 39 208 40 225 2.16e-13 71.2
MS.gene068619.t1 AT4G22110 25.667 300 186 11 39 307 43 336 4.50e-13 70.1
MS.gene068619.t1 AT4G22110 25.667 300 186 11 39 307 43 336 4.50e-13 70.1
MS.gene068619.t1 AT1G64710 25.333 300 194 9 39 310 13 310 9.18e-13 68.9
MS.gene068619.t1 AT1G64710 25.333 300 194 9 39 310 36 333 9.70e-13 69.3
MS.gene068619.t1 AT4G22110 25.581 215 140 5 39 235 43 255 1.37e-11 64.7

Find 95 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCCGTCTTTCCTTTAATTT+TGG 0.091002 5.1:+21455587 MS.gene068619:CDS
ACCAATTGTAGTAGTGTATT+TGG 0.169399 5.1:-21454988 None:intergenic
TTGTTTGGATGTTCAAATTC+AGG 0.242919 5.1:-21454417 None:intergenic
GCTAGCTTACCCAAAATTAA+AGG 0.255595 5.1:-21455597 None:intergenic
GTGAAATTTGCCAAAGCTTT+TGG 0.281096 5.1:+21455233 MS.gene068619:CDS
CCAATTGTAGTAGTGTATTT+GGG 0.295426 5.1:-21454987 None:intergenic
ATCAACCTATCCACTAGTTC+CGG 0.329909 5.1:+21454733 MS.gene068619:CDS
CATCCTATCTTGCCACTACT+TGG 0.340131 5.1:+21455504 MS.gene068619:CDS
GCCCAACCAAAGGCCTTGTT+TGG 0.342284 5.1:-21454432 None:intergenic
GCTGCACCTCTCCTTTGTGC+TGG 0.348614 5.1:+21455116 MS.gene068619:CDS
GATGCTTCCAATTCCACTTC+CGG 0.349243 5.1:-21455775 None:intergenic
GTTGTTGGTCTTGGTGGGCT+TGG 0.363937 5.1:+21455200 MS.gene068619:CDS
CCCAAATACACTACTACAAT+TGG 0.369731 5.1:+21454987 MS.gene068619:CDS
GTCAAAACTTTCAGAGAAAC+AGG 0.379942 5.1:+21454629 MS.gene068619:intron
CTGGTAGCCGGAAGTGGAAT+TGG 0.383425 5.1:+21455768 MS.gene068619:CDS
GACACGAAAATTTAAGAACT+TGG 0.385264 5.1:-21455954 None:intergenic
GATATTTGACATCTGCTTTG+AGG 0.389547 5.1:-21455899 None:intergenic
CATATCGGTGTTGTTGGTCT+TGG 0.391909 5.1:+21455191 MS.gene068619:CDS
GCTAGCAGATGTACTAATTA+CGG 0.396987 5.1:-21455267 None:intergenic
CACATTAGCACCAAAAGCTT+TGG 0.409643 5.1:-21455243 None:intergenic
TCTTGGTGGGCTTGGTCACA+TGG 0.411120 5.1:+21455208 MS.gene068619:CDS
TACAGTCCTCTTAGATACTT+TGG 0.411424 5.1:+21455149 MS.gene068619:CDS
GAACATCCAAACAAGGCCTT+TGG 0.412216 5.1:+21454426 MS.gene068619:CDS
ATCGGTGTTGTTGGTCTTGG+TGG 0.419928 5.1:+21455194 MS.gene068619:CDS
CTGCACCTCTCCTTTGTGCT+GGG 0.424848 5.1:+21455117 MS.gene068619:CDS
CGGAGGTTATTCTGACTCAT+TGG 0.425148 5.1:+21455040 MS.gene068619:CDS
ATAGTCTGCACTAGGTACTT+TGG 0.427527 5.1:+21455452 MS.gene068619:intron
TCAACCTATCCACTAGTTCC+GGG 0.430778 5.1:+21454734 MS.gene068619:CDS
TCCAAACAAGGCCTTTGGTT+GGG 0.431952 5.1:+21454431 MS.gene068619:CDS
GGTCTTCATATCGGTGTTGT+TGG 0.432442 5.1:+21455185 MS.gene068619:CDS
CCCAAAATTAAAGGAAAGAC+GGG 0.434247 5.1:-21455588 None:intergenic
CCCCTTTCAACTTCTCAAGA+AGG 0.434257 5.1:+21454481 MS.gene068619:CDS
AATCCAAGTAGTGGCAAGAT+AGG 0.440808 5.1:-21455507 None:intergenic
GACAAACCTGGTCTTCATAT+CGG 0.456603 5.1:+21455176 MS.gene068619:CDS
GGGATCTCCAGAGGCTTGTC+AGG 0.464857 5.1:-21455567 None:intergenic
GCTTCAAGTGGTAAGCCATC+AGG 0.468059 5.1:-21455092 None:intergenic
GATAGGATGATTTGCTGAAA+CGG 0.468221 5.1:-21455490 None:intergenic
AGAAAGCTGGTAGCCGGAAG+TGG 0.469070 5.1:+21455762 MS.gene068619:CDS
TTATTCTGACTCATTGGTTG+CGG 0.471466 5.1:+21455046 MS.gene068619:CDS
AACCTTCTTGAGAAGTTGAA+AGG 0.479627 5.1:-21454483 None:intergenic
ACCTTCTTGAGAAGTTGAAA+GGG 0.480830 5.1:-21454482 None:intergenic
TACATCTGCTAGCAAAGAAA+AGG 0.482166 5.1:+21455277 MS.gene068619:CDS
TATCGATTTGTGCTCGACAT+TGG 0.486206 5.1:+21455918 MS.gene068619:CDS
AAATACAGGCATGAGATTGT+TGG 0.500255 5.1:+21454840 MS.gene068619:intron
TGGTGTACCTGACAAGCCTC+TGG 0.501156 5.1:+21455560 MS.gene068619:CDS
AAAGTAGAAAAGTTCAATGT+TGG 0.511069 5.1:+21454885 MS.gene068619:CDS
CTGCATGGCGAAGAACGAAT+GGG 0.515871 5.1:+21454706 MS.gene068619:CDS
TCGAGTCCAAAGTATCTAAG+AGG 0.516519 5.1:-21455155 None:intergenic
GTCTCATGGAAAACTTGTAA+TGG 0.519845 5.1:+21455536 MS.gene068619:CDS
ATCCAAACAAGGCCTTTGGT+TGG 0.532747 5.1:+21454430 MS.gene068619:CDS
CTTGGATTACTAAAGTCTCA+TGG 0.536469 5.1:+21455522 MS.gene068619:CDS
TCTGCATGGCGAAGAACGAA+TGG 0.536596 5.1:+21454705 MS.gene068619:CDS
GCAGGGAGAAAGCTGGTAGC+CGG 0.543654 5.1:+21455756 MS.gene068619:intron
TCGGTGTTGTTGGTCTTGGT+GGG 0.546049 5.1:+21455195 MS.gene068619:CDS
GTTGGTATAGTGACAAAGGT+GGG 0.549964 5.1:+21454858 MS.gene068619:CDS
TGGGCAGCTAGAGACACATC+TGG 0.550336 5.1:+21454450 MS.gene068619:CDS
ACAGGAACGACTTCAATGTC+AGG 0.550360 5.1:-21455846 None:intergenic
ACCCAAAATTAAAGGAAAGA+CGG 0.568562 5.1:-21455589 None:intergenic
TACGTGGTTCACATTCCTGA+TGG 0.574374 5.1:+21455077 MS.gene068619:CDS
GTCTCTAGCTGCCCAACCAA+AGG 0.577379 5.1:-21454442 None:intergenic
TACTTTGGACTCGACAAACC+TGG 0.577584 5.1:+21455164 MS.gene068619:CDS
ATTGGTGCCAAGCATCGTGA+TGG 0.582249 5.1:+21455005 MS.gene068619:CDS
GTTGGAGACAAAGTAGGTGT+TGG 0.583429 5.1:+21454903 MS.gene068619:CDS
CATGGAAAACTTGTAATGGT+TGG 0.586324 5.1:+21455540 MS.gene068619:CDS
GATGGTTCCATCACGATGCT+TGG 0.587249 5.1:-21455012 None:intergenic
TTCAATGTTGGAGACAAAGT+AGG 0.588338 5.1:+21454897 MS.gene068619:CDS
AAGGAAGCAAAAGAACACTT+AGG 0.590978 5.1:+21455296 MS.gene068619:CDS
AAGTGGAATTGGAAGCATCA+AGG 0.592918 5.1:+21455779 MS.gene068619:CDS
ATTATCGGCAGGGAGAAAGC+TGG 0.593426 5.1:+21455749 MS.gene068619:intron
AGAACATACCCCGGAACTAG+TGG 0.597931 5.1:-21454743 None:intergenic
CGTGATGGAACCATCACATA+CGG 0.603134 5.1:+21455020 MS.gene068619:CDS
GACTTTAGTAATCCAAGTAG+TGG 0.603416 5.1:-21455516 None:intergenic
AGAATAACCTCCGTATGTGA+TGG 0.606221 5.1:-21455030 None:intergenic
TGTTGGTATAGTGACAAAGG+TGG 0.606925 5.1:+21454857 MS.gene068619:CDS
CAATTGTAGTAGTGTATTTG+GGG 0.619152 5.1:-21454986 None:intergenic
TGACATTGAAGTCGTTCCTG+TGG 0.621293 5.1:+21455848 MS.gene068619:CDS
TCGTGACCAAGATAAAATGC+AGG 0.622175 5.1:+21455340 MS.gene068619:CDS
CCTTCTTGAGAAGTTGAAAG+GGG 0.624053 5.1:-21454481 None:intergenic
GTGATCCCAGCACAAAGGAG+AGG 0.625373 5.1:-21455122 None:intergenic
GCATTCAAAATATTGTATTG+TGG 0.625490 5.1:+21454665 MS.gene068619:CDS
TCTGCACTAGGTACTTTGGA+TGG 0.633602 5.1:+21455456 MS.gene068619:CDS
ATACCCCGGAACTAGTGGAT+AGG 0.638219 5.1:-21454738 None:intergenic
AGAGGTGCAGCAGCTTCAAG+TGG 0.642094 5.1:-21455104 None:intergenic
ATGTCACTCAGATGTCTGCA+TGG 0.642700 5.1:+21454691 MS.gene068619:CDS
GCTGTGTTAACATAATCCAC+AGG 0.647825 5.1:-21455864 None:intergenic
GGAAAGACGGGGATCTCCAG+AGG 0.652974 5.1:-21455576 None:intergenic
TGCATGGCGAAGAACGAATG+GGG 0.656200 5.1:+21454707 MS.gene068619:CDS
GATTGTTGGTATAGTGACAA+AGG 0.656994 5.1:+21454854 MS.gene068619:CDS
CAACCTATCCACTAGTTCCG+GGG 0.659512 5.1:+21454735 MS.gene068619:CDS
ACAACACCGATATGAAGACC+AGG 0.677179 5.1:-21455182 None:intergenic
CCAAAATTAAAGGAAAGACG+GGG 0.687666 5.1:-21455587 None:intergenic
TGAATTTGAACATCCAAACA+AGG 0.697834 5.1:+21454419 MS.gene068619:CDS
ACACAGTGATCCCAGCACAA+AGG 0.705727 5.1:-21455127 None:intergenic
GATGGAACCATCACATACGG+AGG 0.744602 5.1:+21455023 MS.gene068619:CDS
GGTTGCGGATGAGAACTACG+TGG 0.814100 5.1:+21455061 MS.gene068619:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAAAAAAGTGAAG+TGG - chr5.1:21454519-21454538 None:intergenic 15.0%
!! CTAATTTATATAAGAAATAC+AGG + chr5.1:21454826-21454845 MS.gene068619:intron 15.0%
!! TAGTTCAATATTCTATTATA+CGG + chr5.1:21455374-21455393 MS.gene068619:intron 15.0%
!! CAAATCAAAATAGTAGAAAA+TGG - chr5.1:21454803-21454822 None:intergenic 20.0%
!! TTAATCATATAAGTGATCTA+TGG - chr5.1:21455642-21455661 None:intergenic 20.0%
!! TTTATGATGCTAACAATTAT+CGG + chr5.1:21455734-21455753 MS.gene068619:intron 20.0%
!!! TTAAGAGTAAAATTGTTGTT+AGG + chr5.1:21454581-21454600 MS.gene068619:intron 20.0%
! AAAGTAGAAAAGTTCAATGT+TGG + chr5.1:21454885-21454904 MS.gene068619:CDS 25.0%
! AATTTAATTAGAACATACCC+CGG - chr5.1:21454755-21454774 None:intergenic 25.0%
! GCATTCAAAATATTGTATTG+TGG + chr5.1:21454665-21454684 MS.gene068619:CDS 25.0%
! TAATTAGTTCACGAATTGAT+TGG - chr5.1:21455418-21455437 None:intergenic 25.0%
ACCAATTGTAGTAGTGTATT+TGG - chr5.1:21454991-21455010 None:intergenic 30.0%
ACCCAAAATTAAAGGAAAGA+CGG - chr5.1:21455592-21455611 None:intergenic 30.0%
CAATTGTAGTAGTGTATTTG+GGG - chr5.1:21454989-21455008 None:intergenic 30.0%
CCAATTGTAGTAGTGTATTT+GGG - chr5.1:21454990-21455009 None:intergenic 30.0%
TGAATTTGAACATCCAAACA+AGG + chr5.1:21454419-21454438 MS.gene068619:CDS 30.0%
TTGTTTGGATGTTCAAATTC+AGG - chr5.1:21454420-21454439 None:intergenic 30.0%
! TTTGTACCTGCATTTTATCT+TGG - chr5.1:21455349-21455368 None:intergenic 30.0%
!! ATGTTTTTATAGTCTGCACT+AGG + chr5.1:21455444-21455463 MS.gene068619:intron 30.0%
!! TGAAAGTTTTGACACATCAA+AGG - chr5.1:21454622-21454641 None:intergenic 30.0%
AAATACAGGCATGAGATTGT+TGG + chr5.1:21454840-21454859 MS.gene068619:intron 35.0%
AACCTTCTTGAGAAGTTGAA+AGG - chr5.1:21454486-21454505 None:intergenic 35.0%
AAGGAAGCAAAAGAACACTT+AGG + chr5.1:21455296-21455315 MS.gene068619:CDS 35.0%
ACCTTCTTGAGAAGTTGAAA+GGG - chr5.1:21454485-21454504 None:intergenic 35.0%
AGAAACAGGTGAAAAAGATG+TGG + chr5.1:21454643-21454662 MS.gene068619:CDS 35.0%
CATGGAAAACTTGTAATGGT+TGG + chr5.1:21455540-21455559 MS.gene068619:CDS 35.0%
CCAAAATTAAAGGAAAGACG+GGG - chr5.1:21455590-21455609 None:intergenic 35.0%
CCCAAAATTAAAGGAAAGAC+GGG - chr5.1:21455591-21455610 None:intergenic 35.0%
CCCAAATACACTACTACAAT+TGG + chr5.1:21454987-21455006 MS.gene068619:CDS 35.0%
CTTGGATTACTAAAGTCTCA+TGG + chr5.1:21455522-21455541 MS.gene068619:CDS 35.0%
GACTTTAGTAATCCAAGTAG+TGG - chr5.1:21455519-21455538 None:intergenic 35.0%
GATATTTGACATCTGCTTTG+AGG - chr5.1:21455902-21455921 None:intergenic 35.0%
GATTGTTGGTATAGTGACAA+AGG + chr5.1:21454854-21454873 MS.gene068619:CDS 35.0%
GCTAGCAGATGTACTAATTA+CGG - chr5.1:21455270-21455289 None:intergenic 35.0%
GTCAAAACTTTCAGAGAAAC+AGG + chr5.1:21454629-21454648 MS.gene068619:intron 35.0%
GTCTCATGGAAAACTTGTAA+TGG + chr5.1:21455536-21455555 MS.gene068619:CDS 35.0%
TACAGTCCTCTTAGATACTT+TGG + chr5.1:21455149-21455168 MS.gene068619:CDS 35.0%
TACATCTGCTAGCAAAGAAA+AGG + chr5.1:21455277-21455296 MS.gene068619:CDS 35.0%
TTCAATGTTGGAGACAAAGT+AGG + chr5.1:21454897-21454916 MS.gene068619:CDS 35.0%
! GCTAGCTTACCCAAAATTAA+AGG - chr5.1:21455600-21455619 None:intergenic 35.0%
! GTGAAATTTGCCAAAGCTTT+TGG + chr5.1:21455233-21455252 MS.gene068619:CDS 35.0%
! TTATTCTGACTCATTGGTTG+CGG + chr5.1:21455046-21455065 MS.gene068619:CDS 35.0%
!! CCCGTCTTTCCTTTAATTTT+GGG + chr5.1:21455588-21455607 MS.gene068619:CDS 35.0%
!! GATAGGATGATTTGCTGAAA+CGG - chr5.1:21455493-21455512 None:intergenic 35.0%
AATCCAAGTAGTGGCAAGAT+AGG - chr5.1:21455510-21455529 None:intergenic 40.0%
AGAATAACCTCCGTATGTGA+TGG - chr5.1:21455033-21455052 None:intergenic 40.0%
ATAGTCTGCACTAGGTACTT+TGG + chr5.1:21455452-21455471 MS.gene068619:intron 40.0%
ATCAACCTATCCACTAGTTC+CGG + chr5.1:21454733-21454752 MS.gene068619:CDS 40.0%
CACATTAGCACCAAAAGCTT+TGG - chr5.1:21455246-21455265 None:intergenic 40.0%
CCTTCTTGAGAAGTTGAAAG+GGG - chr5.1:21454484-21454503 None:intergenic 40.0%
GATGCTAACAATTATCGGCA+GGG + chr5.1:21455739-21455758 MS.gene068619:intron 40.0%
GCTGTGTTAACATAATCCAC+AGG - chr5.1:21455867-21455886 None:intergenic 40.0%
GTTGGTATAGTGACAAAGGT+GGG + chr5.1:21454858-21454877 MS.gene068619:CDS 40.0%
TCGTGACCAAGATAAAATGC+AGG + chr5.1:21455340-21455359 MS.gene068619:CDS 40.0%
TGATGCTAACAATTATCGGC+AGG + chr5.1:21455738-21455757 MS.gene068619:intron 40.0%
TGTTGGTATAGTGACAAAGG+TGG + chr5.1:21454857-21454876 MS.gene068619:CDS 40.0%
! GACAAACCTGGTCTTCATAT+CGG + chr5.1:21455176-21455195 MS.gene068619:CDS 40.0%
! TCGAGTCCAAAGTATCTAAG+AGG - chr5.1:21455158-21455177 None:intergenic 40.0%
!! AAGTGGAATTGGAAGCATCA+AGG + chr5.1:21455779-21455798 MS.gene068619:CDS 40.0%
!! CCCCGTCTTTCCTTTAATTT+TGG + chr5.1:21455587-21455606 MS.gene068619:CDS 40.0%
!! TATCGATTTGTGCTCGACAT+TGG + chr5.1:21455918-21455937 MS.gene068619:CDS 40.0%
ACAACACCGATATGAAGACC+AGG - chr5.1:21455185-21455204 None:intergenic 45.0%
ACAGGAACGACTTCAATGTC+AGG - chr5.1:21455849-21455868 None:intergenic 45.0%
ATCCAAACAAGGCCTTTGGT+TGG + chr5.1:21454430-21454449 MS.gene068619:CDS 45.0%
ATGTCACTCAGATGTCTGCA+TGG + chr5.1:21454691-21454710 MS.gene068619:CDS 45.0%
CATCCTATCTTGCCACTACT+TGG + chr5.1:21455504-21455523 MS.gene068619:CDS 45.0%
CCCCTTTCAACTTCTCAAGA+AGG + chr5.1:21454481-21454500 MS.gene068619:CDS 45.0%
CGGAGGTTATTCTGACTCAT+TGG + chr5.1:21455040-21455059 MS.gene068619:CDS 45.0%
GAACATCCAAACAAGGCCTT+TGG + chr5.1:21454426-21454445 MS.gene068619:CDS 45.0%
GATGCTTCCAATTCCACTTC+CGG - chr5.1:21455778-21455797 None:intergenic 45.0%
GTTGGAGACAAAGTAGGTGT+TGG + chr5.1:21454903-21454922 MS.gene068619:CDS 45.0%
TACGTGGTTCACATTCCTGA+TGG + chr5.1:21455077-21455096 MS.gene068619:CDS 45.0%
TACTTTGGACTCGACAAACC+TGG + chr5.1:21455164-21455183 MS.gene068619:CDS 45.0%
TCAACCTATCCACTAGTTCC+GGG + chr5.1:21454734-21454753 MS.gene068619:CDS 45.0%
TCCAAACAAGGCCTTTGGTT+GGG + chr5.1:21454431-21454450 MS.gene068619:CDS 45.0%
TCTGCACTAGGTACTTTGGA+TGG + chr5.1:21455456-21455475 MS.gene068619:CDS 45.0%
TGACATTGAAGTCGTTCCTG+TGG + chr5.1:21455848-21455867 MS.gene068619:CDS 45.0%
! CGTGATGGAACCATCACATA+CGG + chr5.1:21455020-21455039 MS.gene068619:CDS 45.0%
!! CATATCGGTGTTGTTGGTCT+TGG + chr5.1:21455191-21455210 MS.gene068619:CDS 45.0%
!! GGTCTTCATATCGGTGTTGT+TGG + chr5.1:21455185-21455204 MS.gene068619:CDS 45.0%
ACACAGTGATCCCAGCACAA+AGG - chr5.1:21455130-21455149 None:intergenic 50.0%
AGAACATACCCCGGAACTAG+TGG - chr5.1:21454746-21454765 None:intergenic 50.0%
ATTATCGGCAGGGAGAAAGC+TGG + chr5.1:21455749-21455768 MS.gene068619:intron 50.0%
CAACCTATCCACTAGTTCCG+GGG + chr5.1:21454735-21454754 MS.gene068619:CDS 50.0%
CTGCATGGCGAAGAACGAAT+GGG + chr5.1:21454706-21454725 MS.gene068619:CDS 50.0%
GATGGAACCATCACATACGG+AGG + chr5.1:21455023-21455042 MS.gene068619:CDS 50.0%
GATGGTTCCATCACGATGCT+TGG - chr5.1:21455015-21455034 None:intergenic 50.0%
GCTTCAAGTGGTAAGCCATC+AGG - chr5.1:21455095-21455114 None:intergenic 50.0%
TCTGCATGGCGAAGAACGAA+TGG + chr5.1:21454705-21454724 MS.gene068619:CDS 50.0%
TGCATGGCGAAGAACGAATG+GGG + chr5.1:21454707-21454726 MS.gene068619:CDS 50.0%
! ATACCCCGGAACTAGTGGAT+AGG - chr5.1:21454741-21454760 None:intergenic 50.0%
!! ATCGGTGTTGTTGGTCTTGG+TGG + chr5.1:21455194-21455213 MS.gene068619:CDS 50.0%
!! ATTGGTGCCAAGCATCGTGA+TGG + chr5.1:21455005-21455024 MS.gene068619:CDS 50.0%
!! TCGGTGTTGTTGGTCTTGGT+GGG + chr5.1:21455195-21455214 MS.gene068619:CDS 50.0%
AGAAAGCTGGTAGCCGGAAG+TGG + chr5.1:21455762-21455781 MS.gene068619:CDS 55.0%
CTGCACCTCTCCTTTGTGCT+GGG + chr5.1:21455117-21455136 MS.gene068619:CDS 55.0%
CTGGTAGCCGGAAGTGGAAT+TGG + chr5.1:21455768-21455787 MS.gene068619:CDS 55.0%
GGTTGCGGATGAGAACTACG+TGG + chr5.1:21455061-21455080 MS.gene068619:CDS 55.0%
GTCTCTAGCTGCCCAACCAA+AGG - chr5.1:21454445-21454464 None:intergenic 55.0%
GTGATCCCAGCACAAAGGAG+AGG - chr5.1:21455125-21455144 None:intergenic 55.0%
TGGGCAGCTAGAGACACATC+TGG + chr5.1:21454450-21454469 MS.gene068619:CDS 55.0%
TGGTGTACCTGACAAGCCTC+TGG + chr5.1:21455560-21455579 MS.gene068619:CDS 55.0%
! AGAGGTGCAGCAGCTTCAAG+TGG - chr5.1:21455107-21455126 None:intergenic 55.0%
!! GCCCAACCAAAGGCCTTGTT+TGG - chr5.1:21454435-21454454 None:intergenic 55.0%
!! GTTGTTGGTCTTGGTGGGCT+TGG + chr5.1:21455200-21455219 MS.gene068619:CDS 55.0%
!! TCTTGGTGGGCTTGGTCACA+TGG + chr5.1:21455208-21455227 MS.gene068619:CDS 55.0%
GCAGGGAGAAAGCTGGTAGC+CGG + chr5.1:21455756-21455775 MS.gene068619:intron 60.0%
GCTGCACCTCTCCTTTGTGC+TGG + chr5.1:21455116-21455135 MS.gene068619:CDS 60.0%
GGAAAGACGGGGATCTCCAG+AGG - chr5.1:21455579-21455598 None:intergenic 60.0%
GGGATCTCCAGAGGCTTGTC+AGG - chr5.1:21455570-21455589 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 21454405 21455965 21454405 ID=MS.gene068619
chr5.1 mRNA 21454405 21455965 21454405 ID=MS.gene068619.t1;Parent=MS.gene068619
chr5.1 exon 21454405 21454502 21454405 ID=MS.gene068619.t1.exon1;Parent=MS.gene068619.t1
chr5.1 CDS 21454405 21454502 21454405 ID=cds.MS.gene068619.t1;Parent=MS.gene068619.t1
chr5.1 exon 21454643 21454756 21454643 ID=MS.gene068619.t1.exon2;Parent=MS.gene068619.t1
chr5.1 CDS 21454643 21454756 21454643 ID=cds.MS.gene068619.t1;Parent=MS.gene068619.t1
chr5.1 exon 21454848 21455361 21454848 ID=MS.gene068619.t1.exon3;Parent=MS.gene068619.t1
chr5.1 CDS 21454848 21455361 21454848 ID=cds.MS.gene068619.t1;Parent=MS.gene068619.t1
chr5.1 exon 21455456 21455609 21455456 ID=MS.gene068619.t1.exon4;Parent=MS.gene068619.t1
chr5.1 CDS 21455456 21455609 21455456 ID=cds.MS.gene068619.t1;Parent=MS.gene068619.t1
chr5.1 exon 21455760 21455965 21455760 ID=MS.gene068619.t1.exon5;Parent=MS.gene068619.t1
chr5.1 CDS 21455760 21455965 21455760 ID=cds.MS.gene068619.t1;Parent=MS.gene068619.t1
Gene Sequence

>MS.gene068619

ATGGCAACACAACCTGAATTTGAACATCCAAACAAGGCCTTTGGTTGGGCAGCTAGAGACACATCTGGTGTTCTCTCCCCTTTCAACTTCTCAAGAAGGTTCATTCATACACCACTTCACTTTTTTTTTTTTTTCAATTAATTTATTTTTTACACTTTGATGCATCATATATAGTGTTAAGAGTAAAATTGTTGTTAGGAAAATATATAACTCACCTTTGATGTGTCAAAACTTTCAGAGAAACAGGTGAAAAAGATGTGGCATTCAAAATATTGTATTGTGGAATATGTCACTCAGATGTCTGCATGGCGAAGAACGAATGGGGCATATCAACCTATCCACTAGTTCCGGGGTATGTTCTAATTAAATTATCTTAGAAAAGTCTTAAAGTCTTACCATTTTCTACTATTTTGATTTGATTCTAATTTATATAAGAAATACAGGCATGAGATTGTTGGTATAGTGACAAAGGTGGGAAGCAAAGTAGAAAAGTTCAATGTTGGAGACAAAGTAGGTGTTGGCTACATAATTGATTCATGTCGATCGTGTCAAAATTGTGTTGACAATCTTGAAAATTACTGCCCCAAATACACTACTACAATTGGTGCCAAGCATCGTGATGGAACCATCACATACGGAGGTTATTCTGACTCATTGGTTGCGGATGAGAACTACGTGGTTCACATTCCTGATGGCTTACCACTTGAAGCTGCTGCACCTCTCCTTTGTGCTGGGATCACTGTGTACAGTCCTCTTAGATACTTTGGACTCGACAAACCTGGTCTTCATATCGGTGTTGTTGGTCTTGGTGGGCTTGGTCACATGGCTGTGAAATTTGCCAAAGCTTTTGGTGCTAATGTGACCGTAATTAGTACATCTGCTAGCAAAGAAAAGGAAGCAAAAGAACACTTAGGAGCTGATTCATTTCTGATAAGTCGTGACCAAGATAAAATGCAGGTACAAAAACAATAGTTCAATATTCTATTATACGGAAATTTACAATGCTATTGCCAATCAATTCGTGAACTAATTATGTTAAATGTTTTTATAGTCTGCACTAGGTACTTTGGATGGTATCATTGATACCGTTTCAGCAAATCATCCTATCTTGCCACTACTTGGATTACTAAAGTCTCATGGAAAACTTGTAATGGTTGGTGTACCTGACAAGCCTCTGGAGATCCCCGTCTTTCCTTTAATTTTGGGTAAGCTAGCATATATATGAACTAAGTTTCCATAGATCACTTATATGATTAAAAGCTATAAGTCATATATACATATATAGTATAATGCACATGAGAATATCAATTTAATTTATGAATAAGCATTTTTATGATGCTAACAATTATCGGCAGGGAGAAAGCTGGTAGCCGGAAGTGGAATTGGAAGCATCAAGGAGACTCAAGAGATGCTTGACTTTGCTGCTAAACACAATGTAAAACCTGACATTGAAGTCGTTCCTGTGGATTATGTTAACACAGCAATTGAACGACTCCTCAAAGCAGATGTCAAATATCGATTTGTGCTCGACATTGGAAACACTCTGAAACCAAGTTCTTAA
Protein sequence

>MS.gene068619.t1

MATQPEFEHPNKAFGWAARDTSGVLSPFNFSRRETGEKDVAFKILYCGICHSDVCMAKNEWGISTYPLVPGHEIVGIVTKVGSKVEKFNVGDKVGVGYIIDSCRSCQNCVDNLENYCPKYTTTIGAKHRDGTITYGGYSDSLVADENYVVHIPDGLPLEAAAPLLCAGITVYSPLRYFGLDKPGLHIGVVGLGGLGHMAVKFAKAFGANVTVISTSASKEKEAKEHLGADSFLISRDQDKMQSALGTLDGIIDTVSANHPILPLLGLLKSHGKLVMVGVPDKPLEIPVFPLILGRKLVAGSGIGSIKETQEMLDFAAKHNVKPDIEVVPVDYVNTAIERLLKADVKYRFVLDIGNTLKPSS