AlfalfaGEDB | MS.gene070879

M. sativa cultivar XinJiangDaYe / MS.gene070879

KEGG: -
GO: GO:0003674 GO:0003824 GO:0005488 GO:0008150 GO:0008152 GO:0008270 GO:0016491 GO:0043167 GO:0043169 GO:0046872 GO:0046914 GO:0055114
PFAM: PF00107.29 PF08240.15

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene070879.t1	XP_003612986.1	97.3	329	9	0	7	335	35	363	5.60E-185	656.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene070879.t1	Q9ZRF1	78.2	326	71	0	7	332	31	356	3.7e-153	542.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene070879.t1	G7KGI9	97.3	329	9	0	7	335	35	363	4.0e-185	656.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049195	MS.gene070879	0.809109	2.23E-50	-1.69E-46
MS.gene049993	MS.gene070879	0.835675	1.46E-56	-1.69E-46
MS.gene051208	MS.gene070879	0.808157	3.55E-50	-1.69E-46
MS.gene051525	MS.gene070879	0.839463	1.56E-57	-1.69E-46
MS.gene051582	MS.gene070879	0.874013	9.41E-68	-1.69E-46
MS.gene051588	MS.gene070879	0.803805	2.92E-49	-1.69E-46
MS.gene052011	MS.gene070879	-0.850527	1.60E-60	-1.69E-46
MS.gene053330	MS.gene070879	0.840912	6.53E-58	-1.69E-46
MS.gene053971	MS.gene070879	0.801108	1.05E-48	-1.69E-46
MS.gene054183	MS.gene070879	0.811543	6.64E-51	-1.69E-46
MS.gene054719	MS.gene070879	0.81007	1.38E-50	-1.69E-46
MS.gene054918	MS.gene070879	0.822135	2.78E-53	-1.69E-46
MS.gene055217	MS.gene070879	0.822463	2.34E-53	-1.69E-46
MS.gene055428	MS.gene070879	0.808251	3.40E-50	-1.69E-46
MS.gene05553	MS.gene070879	0.837128	6.24E-57	-1.69E-46
MS.gene055564	MS.gene070879	0.825285	5.09E-54	-1.69E-46
MS.gene056337	MS.gene070879	0.818073	2.37E-52	-1.69E-46
MS.gene056451	MS.gene070879	0.814319	1.64E-51	-1.69E-46
MS.gene056633	MS.gene070879	0.821276	4.40E-53	-1.69E-46
MS.gene05702	MS.gene070879	0.813517	2.46E-51	-1.69E-46
MS.gene057196	MS.gene070879	0.802315	5.95E-49	-1.69E-46
MS.gene058142	MS.gene070879	0.80409	2.55E-49	-1.69E-46
MS.gene058233	MS.gene070879	0.843414	1.42E-58	-1.69E-46
MS.gene058276	MS.gene070879	0.810453	1.14E-50	-1.69E-46
MS.gene058746	MS.gene070879	0.835961	1.24E-56	-1.69E-46
MS.gene05886	MS.gene070879	0.837967	3.80E-57	-1.69E-46
MS.gene060403	MS.gene070879	0.80363	3.18E-49	-1.69E-46
MS.gene061114	MS.gene070879	-0.814065	1.86E-51	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene070868	MS.gene070879	PPI
MS.gene070879	MS.gene027819	PPI
MS.gene070879	MS.gene069144	PPI
MS.gene070879	MS.gene048392	PPI
MS.gene070879	MS.gene070871	PPI
MS.gene070879	MS.gene46316	PPI
MS.gene069145	MS.gene070879	PPI
MS.gene070881	MS.gene070879	PPI
MS.gene068618	MS.gene070879	PPI
MS.gene070879	MS.gene070876	PPI
MS.gene069146	MS.gene070879	PPI
MS.gene070879	MS.gene70270	PPI
MS.gene070879	MS.gene027816	PPI
MS.gene93004	MS.gene070879	PPI
MS.gene070879	MS.gene068620	PPI
MS.gene070879	MS.gene069153	PPI
MS.gene069151	MS.gene070879	PPI
MS.gene027824	MS.gene070879	PPI
MS.gene070879	MS.gene027820	PPI
MS.gene068619	MS.gene070879	PPI
MS.gene070879	MS.gene069147	PPI
MS.gene070879	MS.gene070868	PPI
MS.gene068613	MS.gene070879	PPI
MS.gene070874	MS.gene070879	PPI
MS.gene068614	MS.gene070879	PPI
MS.gene070879	MS.gene070869	PPI
MS.gene027821	MS.gene070879	PPI
MS.gene070879	MS.gene008956	PPI
MS.gene28444	MS.gene070879	PPI
MS.gene58124	MS.gene070879	PPI
MS.gene070879	MS.gene02214	PPI
MS.gene002258	MS.gene070879	PPI
MS.gene027817	MS.gene070879	PPI
MS.gene070879	MS.gene90766	PPI
MS.gene070879	MS.gene070872	PPI
MS.gene070879	MS.gene036724	PPI
MS.gene070879	MS.gene027823	PPI
MS.gene070879	MS.gene069154	PPI
MS.gene070879	MS.gene068621	PPI
MS.gene027818	MS.gene070879	PPI
MS.gene065692	MS.gene070879	PPI
MS.gene069148	MS.gene070879	PPI
MS.gene070879	MS.gene027825	PPI
MS.gene070879	MS.gene069152	PPI
MS.gene070879	MS.gene71681	PPI
MS.gene070879	MS.gene070877	PPI
MS.gene069154	MS.gene070879	PPI
MS.gene068611	MS.gene070879	PPI
MS.gene58045	MS.gene070879	PPI
MS.gene070879	MS.gene01230	PPI
MS.gene030674	MS.gene070879	PPI
MS.gene002257	MS.gene070879	PPI
MS.gene070879	MS.gene029589	PPI
MS.gene068612	MS.gene070879	PPI
MS.gene070879	MS.gene068615	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene070879.t1	MTR_5g031360	97.264	329	9	0	7	335	35	363	0.0	658
MS.gene070879.t1	MTR_5g031310	89.666	329	34	0	7	335	34	362	0.0	593
MS.gene070879.t1	MTR_5g031305	86.930	329	43	0	7	335	34	362	0.0	584
MS.gene070879.t1	MTR_5g031390	80.793	328	63	0	7	334	33	360	0.0	535
MS.gene070879.t1	MTR_5g031300	78.963	328	69	0	7	334	34	361	0.0	534
MS.gene070879.t1	MTR_4g115300	78.354	328	71	0	7	334	33	360	0.0	529
MS.gene070879.t1	MTR_5g031230	78.049	328	72	0	7	334	33	360	0.0	524
MS.gene070879.t1	MTR_5g031250	77.439	328	74	0	7	334	33	360	0.0	516
MS.gene070879.t1	MTR_5g031190	75.915	328	79	0	7	334	33	360	0.0	511
MS.gene070879.t1	MTR_5g031210	75.915	328	79	0	7	334	33	360	1.01e-178	499
MS.gene070879.t1	MTR_8g068190	78.354	328	71	0	7	334	33	360	2.75e-175	490
MS.gene070879.t1	MTR_5g031180	76.220	328	78	0	7	334	33	360	2.18e-171	480
MS.gene070879.t1	MTR_8g092630	62.918	329	121	1	3	331	86	413	2.34e-140	404
MS.gene070879.t1	MTR_5g031430	59.509	326	132	0	7	332	33	358	1.49e-132	382
MS.gene070879.t1	MTR_1g025950	59.385	325	131	1	7	330	32	356	5.24e-127	368
MS.gene070879.t1	MTR_1g107425	53.538	325	151	0	6	330	30	354	2.90e-118	345
MS.gene070879.t1	MTR_5g031250	79.426	209	43	0	7	215	33	241	9.31e-118	340
MS.gene070879.t1	MTR_2g009810	52.599	327	153	2	7	332	81	406	6.81e-106	316
MS.gene070879.t1	MTR_1g107425	55.294	255	114	0	6	260	30	284	1.93e-91	275
MS.gene070879.t1	MTR_2g009820	50.628	239	115	2	97	332	16	254	6.99e-65	206
MS.gene070879.t1	MTR_5g031290	73.913	115	30	0	221	335	80	194	5.30e-53	173
MS.gene070879.t1	MTR_5g031290	79.167	48	9	1	7	53	33	80	9.12e-20	86.3
MS.gene070879.t1	MTR_8g095220	26.496	351	216	13	13	327	80	424	6.03e-22	96.3
MS.gene070879.t1	MTR_8g095220	29.817	218	128	8	21	216	88	302	2.30e-21	93.2
MS.gene070879.t1	MTR_0001s0660	27.077	325	202	11	12	305	35	355	1.49e-20	92.4
MS.gene070879.t1	MTR_5g045710	24.927	341	209	10	12	316	35	364	1.34e-16	80.1
MS.gene070879.t1	MTR_3g089977	22.478	347	234	10	13	328	37	379	6.82e-15	75.1
MS.gene070879.t1	MTR_6g465010	27.723	202	132	5	10	207	22	213	1.68e-14	73.9
MS.gene070879.t1	MTR_3g089940	22.024	336	230	8	13	318	37	370	1.92e-14	73.9
MS.gene070879.t1	MTR_3g089940	22.024	336	230	8	13	318	37	370	1.97e-14	73.9
MS.gene070879.t1	MTR_6g466320	27.723	202	132	5	10	207	38	229	2.16e-14	73.6
MS.gene070879.t1	MTR_6g465010	27.723	202	132	5	10	207	38	229	2.16e-14	73.6
MS.gene070879.t1	MTR_3g089970	21.726	336	231	7	13	318	38	371	1.67e-13	71.2
MS.gene070879.t1	MTR_7g068320	24.854	342	215	11	13	317	48	384	1.91e-13	70.9
MS.gene070879.t1	MTR_3g079180	26.510	298	187	11	12	281	40	333	7.60e-13	69.3
MS.gene070879.t1	MTR_5g087710	24.348	345	213	11	13	318	46	381	8.49e-13	68.9
MS.gene070879.t1	MTR_3g089940	23.707	232	159	3	13	227	37	267	1.02e-12	68.6
MS.gene070879.t1	MTR_3g089940	23.707	232	159	3	13	227	37	267	1.05e-12	68.6
MS.gene070879.t1	MTR_7g068330	25.731	342	211	12	13	317	41	376	1.21e-12	68.6
MS.gene070879.t1	MTR_3g099460	27.476	313	189	11	13	293	37	343	3.25e-12	67.4
MS.gene070879.t1	MTR_3g099460	27.476	313	189	11	13	293	44	350	4.07e-12	67.0
MS.gene070879.t1	MTR_5g031380	60.465	43	17	0	54	96	19	61	4.44e-12	62.4
MS.gene070879.t1	MTR_1g069430	25.397	315	204	8	13	301	36	345	6.23e-12	66.2
MS.gene070879.t1	MTR_7g099040	23.810	315	209	8	13	301	38	347	6.08e-11	63.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene070879.t1	AT4G37980	70.821	329	96	0	7	335	29	357	4.63e-161	454
MS.gene070879.t1	AT4G37990	70.821	329	96	0	7	335	29	357	7.05e-159	449
MS.gene070879.t1	AT4G37970	63.720	328	119	0	7	334	34	361	1.14e-153	436
MS.gene070879.t1	AT4G39330	58.610	331	137	0	3	333	29	359	6.47e-143	408
MS.gene070879.t1	AT2G21730	57.658	333	139	2	3	333	23	355	2.54e-136	392
MS.gene070879.t1	AT2G21890	56.928	332	142	1	3	333	23	354	3.48e-136	392
MS.gene070879.t1	AT4G37980	71.374	262	75	0	7	268	29	290	1.58e-120	349
MS.gene070879.t1	AT4G37970	61.832	262	100	0	7	268	34	295	1.22e-114	334
MS.gene070879.t1	AT4G39330	56.727	275	119	0	3	277	29	303	2.12e-110	324
MS.gene070879.t1	AT3G19450	52.615	325	154	0	6	330	30	354	1.48e-105	313
MS.gene070879.t1	AT1G72680	49.387	326	164	1	7	332	31	355	1.01e-103	308
MS.gene070879.t1	AT4G34230	51.077	325	159	0	6	330	29	353	4.07e-96	289
MS.gene070879.t1	AT4G34230	50.769	325	160	0	6	330	29	353	3.69e-95	286
MS.gene070879.t1	AT4G37970	61.033	213	83	0	7	219	34	246	2.35e-90	271
MS.gene070879.t1	AT4G37970	61.429	210	81	0	7	216	34	243	2.19e-89	268
MS.gene070879.t1	AT5G63620	25.862	348	224	9	12	327	81	426	2.58e-24	103
MS.gene070879.t1	AT5G63620	28.464	267	163	7	12	252	81	345	2.96e-24	102
MS.gene070879.t1	AT5G63620	29.204	226	137	6	12	216	81	304	1.50e-23	99.4
MS.gene070879.t1	AT1G22430	27.609	297	184	9	13	281	42	335	9.52e-19	86.7
MS.gene070879.t1	AT1G22430	27.609	297	184	9	13	281	42	335	9.52e-19	86.7
MS.gene070879.t1	AT5G43940	25.648	347	219	10	13	326	48	388	5.35e-18	84.3
MS.gene070879.t1	AT5G43940	25.648	347	219	10	13	326	36	376	5.37e-18	84.3
MS.gene070879.t1	AT1G64710	26.254	339	213	13	13	317	13	348	5.73e-18	84.0
MS.gene070879.t1	AT1G64710	26.254	339	213	13	13	317	36	371	8.22e-18	84.0
MS.gene070879.t1	AT1G32780	26.039	361	222	12	13	332	37	393	4.10e-17	82.0
MS.gene070879.t1	AT1G22440	24.058	345	215	13	13	318	40	376	6.39e-17	81.3
MS.gene070879.t1	AT5G51970	25.098	255	170	6	10	252	40	285	1.50e-15	77.0
MS.gene070879.t1	AT5G51970	25.098	255	170	6	10	252	40	285	1.50e-15	77.0
MS.gene070879.t1	AT1G22430	27.136	199	126	5	13	193	42	239	3.43e-15	75.5
MS.gene070879.t1	AT1G22430	27.136	199	126	5	13	193	42	239	5.96e-15	74.7
MS.gene070879.t1	AT1G22430	27.136	199	126	5	13	193	42	239	5.96e-15	74.7
MS.gene070879.t1	AT4G22110	24.561	342	215	14	13	317	43	378	8.51e-15	75.1
MS.gene070879.t1	AT4G22110	24.561	342	215	14	13	317	43	378	8.51e-15	75.1
MS.gene070879.t1	AT1G77120	22.609	345	236	9	13	328	36	378	1.07e-13	71.6
MS.gene070879.t1	AT5G42250	24.716	352	223	13	13	328	45	390	1.18e-13	71.6
MS.gene070879.t1	AT4G22110	25.126	199	130	6	13	193	43	240	4.66e-12	65.9

Find 81 sgRNAs with CRISPR-Local

Find 99 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATTGAATGTTTGAGTATATT+TGG	0.100274	5.4:+21462161	None:intergenic
TCAATAGCTGGAAGTTTAAT+TGG	0.184530	5.4:-21461442	MS.gene070879:CDS
TATAGTCCTCTTCGACATTT+TGG	0.197168	5.4:-21462000	MS.gene070879:CDS
TATCGATTCGTGATTGATAT+TGG	0.245698	5.4:-21461292	MS.gene070879:CDS
TTGAATGTTTGAGTATATTT+GGG	0.265617	5.4:+21462162	None:intergenic
GTGAAATTCGCCAAAGCTTT+TGG	0.268679	5.4:-21461916	MS.gene070879:CDS
ATTGGTTTATTGAAATCTAA+TGG	0.271827	5.4:-21461642	MS.gene070879:CDS
AATTAGTACATCACCTAAAA+AGG	0.281212	5.4:-21461878	MS.gene070879:CDS
CCTGATAGCTTACCTCTTGA+TGG	0.315007	5.4:-21462057	MS.gene070879:CDS
TTATGGTGCATCAAGACTTC+AGG	0.343023	5.4:-21462652	MS.gene070879:CDS
TTTAGGTGATGTACTAATTA+CGG	0.352263	5.4:+21461882	None:intergenic
CACTTTAGCACCAAAAGCTT+TGG	0.357444	5.4:+21461906	None:intergenic
AAGTTTAATTGGAGGGATAA+AGG	0.373525	5.4:-21461431	MS.gene070879:CDS
TCTTGGTGGACTTGGTCATA+TGG	0.375197	5.4:-21461941	MS.gene070879:CDS
CATCAAGACTTCAGGGAAAC+AGG	0.379283	5.4:-21462644	MS.gene070879:CDS
AAACCAATCAATGGTAAAAC+AGG	0.382671	5.4:+21461657	None:intergenic
TAAATACAGGCACGAACTTA+CGG	0.392066	5.4:-21462310	MS.gene070879:intron
AAAACATACCCCGGGACTAA+TGG	0.397804	5.4:+21462530	None:intergenic
TTTAAAGTTGGAGACAAAGT+AGG	0.401632	5.4:-21462252	MS.gene070879:CDS
CCATCAAGAGGTAAGCTATC+AGG	0.415030	5.4:+21462057	None:intergenic
GTTGTCGGTCTTGGTGGACT+TGG	0.416664	5.4:-21461949	MS.gene070879:CDS
ATTAATACCTGCATCTGATC+TGG	0.419726	5.4:+21461802	None:intergenic
AAATACAGGCACGAACTTAC+GGG	0.426387	5.4:-21462309	MS.gene070879:intron
CGCGATGGCACCATCACCTA+TGG	0.430946	5.4:-21462129	MS.gene070879:CDS
TTCACATTATGCAGAATGAA+TGG	0.433959	5.4:-21462568	MS.gene070879:CDS
GCCGGTCCTCTTCTTTGCGC+TGG	0.434288	5.4:-21462033	MS.gene070879:CDS
CATTCAAGGTGTTGTATTGT+GGG	0.442854	5.4:-21462607	MS.gene070879:CDS
TTAAAGTTGGAGACAAAGTA+GGG	0.443890	5.4:-21462251	MS.gene070879:CDS
TGAGAAAGATGTAGCATTCA+AGG	0.455978	5.4:-21462621	MS.gene070879:CDS
CAAAGTAGGGGTGGGATACA+TGG	0.457657	5.4:-21462238	MS.gene070879:CDS
AATATAGGAGTTGTCGGTCT+TGG	0.461840	5.4:-21461958	MS.gene070879:CDS
GACAAGCCTGGTATGAATAT+AGG	0.464749	5.4:-21461973	MS.gene070879:CDS
TCACATTATGCAGAATGAAT+GGG	0.468998	5.4:-21462567	MS.gene070879:CDS
ATAGGAGTTGTCGGTCTTGG+TGG	0.469917	5.4:-21461955	MS.gene070879:CDS
AAATATACTCAAACATTCAA+TGG	0.483631	5.4:-21462159	MS.gene070879:CDS
CCTCCAAGTAAAGAAAATAC+AGG	0.487127	5.4:+21461576	None:intergenic
GATTTCAATAAACCAATCAA+TGG	0.487235	5.4:+21461648	None:intergenic
CAATGTAACTCCTGAGATTG+AGG	0.489775	5.4:-21461374	MS.gene070879:CDS
TGGAGGTTTCTCCGACTCAA+TGG	0.497539	5.4:-21462109	MS.gene070879:CDS
GTAGGGAGAAAGTCAATAGC+TGG	0.498727	5.4:-21461454	MS.gene070879:intron
AACCACCTATCCATTAGTCC+CGG	0.500566	5.4:-21462540	MS.gene070879:CDS
ATAGGAACAACCTCAATCTC+AGG	0.503943	5.4:+21461364	None:intergenic
GCAATGGAGCGTCTTGCTAA+AGG	0.517863	5.4:-21461325	MS.gene070879:CDS
GCATTCAAGGTGTTGTATTG+TGG	0.522756	5.4:-21462608	MS.gene070879:CDS
GGTATGAATATAGGAGTTGT+CGG	0.535538	5.4:-21461964	MS.gene070879:CDS
AGCTTACCTCTTGATGGCGC+CGG	0.538501	5.4:-21462051	MS.gene070879:CDS
ACCACCTATCCATTAGTCCC+GGG	0.540962	5.4:-21462539	MS.gene070879:CDS
TATGGTGCATCAAGACTTCA+GGG	0.545368	5.4:-21462651	MS.gene070879:CDS
GAAAATACAGGAAGCTCTAG+AGG	0.546279	5.4:+21461588	None:intergenic
AGTTGGAGACAAAGTAGGGG+TGG	0.553693	5.4:-21462247	MS.gene070879:CDS
TCCAGCGCAAAGAAGAGGAC+CGG	0.556041	5.4:+21462032	None:intergenic
GTGACTCCAGCGCAAAGAAG+AGG	0.566494	5.4:+21462027	None:intergenic
TAGCTGGAAGTTTAATTGGA+GGG	0.567406	5.4:-21461438	MS.gene070879:CDS
GCTGCAACGAGTACTTTGAA+TGG	0.585867	5.4:-21461708	MS.gene070879:CDS
ATCTAATGGAAAGCTTGTAA+TGG	0.587781	5.4:-21461628	MS.gene070879:CDS
CGATTATGTCAACACAGCAA+TGG	0.588395	5.4:-21461341	MS.gene070879:CDS
ATAGCTGGAAGTTTAATTGG+AGG	0.588996	5.4:-21461439	MS.gene070879:CDS
CTCATCTGCAACCATTGAGT+CGG	0.590003	5.4:+21462098	None:intergenic
AAGGAAGCAATAGAACACTT+AGG	0.595080	5.4:-21461853	MS.gene070879:CDS
AGAGGACCGGCGCCATCAAG+AGG	0.596002	5.4:+21462045	None:intergenic
CCGCGATCCAGATCAGATGC+AGG	0.596450	5.4:-21461809	MS.gene070879:intron
CACATTATGCAGAATGAATG+GGG	0.597941	5.4:-21462566	MS.gene070879:CDS
GGAGAAACCTCCATAGGTGA+TGG	0.604248	5.4:+21462119	None:intergenic
GTTGGAGACAAAGTAGGGGT+GGG	0.608564	5.4:-21462246	MS.gene070879:CDS
TTCAATGGTAAGTATCGCGA+TGG	0.612763	5.4:-21462144	MS.gene070879:CDS
ATACCCCGGGACTAATGGAT+AGG	0.615005	5.4:+21462535	None:intergenic
AATGGAAAGCTTGTAATGGT+TGG	0.618001	5.4:-21461624	MS.gene070879:CDS
CCCCGGGACTAATGGATAGG+TGG	0.620100	5.4:+21462538	None:intergenic
TGAATGTTTGAGTATATTTG+GGG	0.623863	5.4:+21462163	None:intergenic
ATTCATTCTGCATAATGTGA+AGG	0.626134	5.4:+21462570	None:intergenic
TCAAGTCCAAAATGTCGAAG+AGG	0.630107	5.4:+21461994	None:intergenic
TACGGGTGTAGTAACAGAGG+TGG	0.634996	5.4:-21462292	MS.gene070879:CDS
TGAGTCGGAGAAACCTCCAT+AGG	0.648832	5.4:+21462113	None:intergenic
ACGGGTGTAGTAACAGAGGT+GGG	0.650453	5.4:-21462291	MS.gene070879:CDS
GCTGTGTTGACATAATCGAT+AGG	0.651012	5.4:+21461346	None:intergenic
CCTGCATCTGATCTGGATCG+CGG	0.652791	5.4:+21461809	None:intergenic
TAAAGTTGGAGACAAAGTAG+GGG	0.656574	5.4:-21462250	MS.gene070879:CDS
ACAACTCCTATATTCATACC+AGG	0.672537	5.4:+21461967	None:intergenic
GATGGCACCATCACCTATGG+AGG	0.673821	5.4:-21462126	MS.gene070879:CDS
CCACCTATCCATTAGTCCCG+GGG	0.685161	5.4:-21462538	MS.gene070879:intron
ACTTACGGGTGTAGTAACAG+AGG	0.741818	5.4:-21462295	MS.gene070879:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATAAAATAATTGATAAAGTA+AGG	+	chr5.4:21461504-21461523	None:intergenic	10.0%
!!	TAAAATAATTGATAAAGTAA+GGG	+	chr5.4:21461503-21461522	None:intergenic	10.0%
!!	TAAACTATAATAGAAGTTAA+AGG	+	chr5.4:21461418-21461437	None:intergenic	15.0%
!!!	AAAGTAAAAAAGTTTAAAGT+TGG	-	chr5.4:21461653-21461672	MS.gene070879:CDS	15.0%
!!	AAATATACTCAAACATTCAA+TGG	-	chr5.4:21461758-21461777	MS.gene070879:intron	20.0%
!!	AATATTGTACAACATTTCAT+AGG	-	chr5.4:21462187-21462206	MS.gene070879:CDS	20.0%
!!	ATAACTGATGTAATAAATAC+AGG	-	chr5.4:21461594-21461613	MS.gene070879:CDS	20.0%
!!	ATTGAATGTTTGAGTATATT+TGG	+	chr5.4:21461759-21461778	None:intergenic	20.0%
!!	TAGAAAATAATTGCTGAATT+TGG	-	chr5.4:21461569-21461588	MS.gene070879:intron	20.0%
!!	TTGAATGTTTGAGTATATTT+GGG	+	chr5.4:21461758-21461777	None:intergenic	20.0%
!!	TTGTATTAATTTCGCATTAT+TGG	-	chr5.4:21462441-21462460	MS.gene070879:intron	20.0%
!!!	ATTGGTTTATTGAAATCTAA+TGG	-	chr5.4:21462275-21462294	MS.gene070879:CDS	20.0%
!	AATTAGTACATCACCTAAAA+AGG	-	chr5.4:21462039-21462058	MS.gene070879:CDS	25.0%
!	ACGTGACAATTAAAAAGAAA+CGG	+	chr5.4:21461446-21461465	None:intergenic	25.0%
!	ATTAATTTCGCATTATTGGT+AGG	-	chr5.4:21462445-21462464	MS.gene070879:intron	25.0%
!	GATTTCAATAAACCAATCAA+TGG	+	chr5.4:21462272-21462291	None:intergenic	25.0%
!	TGAATGTTTGAGTATATTTG+GGG	+	chr5.4:21461757-21461776	None:intergenic	25.0%
!	TTAATTTCGCATTATTGGTA+GGG	-	chr5.4:21462446-21462465	MS.gene070879:intron	25.0%
!	TTTAGGTGATGTACTAATTA+CGG	+	chr5.4:21462038-21462057	None:intergenic	25.0%
	AAACCAATCAATGGTAAAAC+AGG	+	chr5.4:21462263-21462282	None:intergenic	30.0%
	AAGTTTAATTGGAGGGATAA+AGG	-	chr5.4:21462486-21462505	MS.gene070879:intron	30.0%
	ATCTAATGGAAAGCTTGTAA+TGG	-	chr5.4:21462289-21462308	MS.gene070879:CDS	30.0%
	ATTCATTCTGCATAATGTGA+AGG	+	chr5.4:21461350-21461369	None:intergenic	30.0%
	TACATCACCTAAAAAGGAAA+AGG	-	chr5.4:21462045-21462064	MS.gene070879:CDS	30.0%
	TCAATAGCTGGAAGTTTAAT+TGG	-	chr5.4:21462475-21462494	MS.gene070879:intron	30.0%
	TCACATTATGCAGAATGAAT+GGG	-	chr5.4:21461350-21461369	MS.gene070879:CDS	30.0%
	TCAGATGCAGGTATTAATTT+CGG	-	chr5.4:21462120-21462139	MS.gene070879:CDS	30.0%
	TTAAAGTTGGAGACAAAGTA+GGG	-	chr5.4:21461666-21461685	MS.gene070879:CDS	30.0%
	TTCACATTATGCAGAATGAA+TGG	-	chr5.4:21461349-21461368	MS.gene070879:CDS	30.0%
!	ATTGCTTCCTTTTCCTTTTT+AGG	+	chr5.4:21462055-21462074	None:intergenic	30.0%
!	CTTCCTGTATTTTCTTTACT+TGG	-	chr5.4:21462338-21462357	MS.gene070879:intron	30.0%
!	TTTAAAGTTGGAGACAAAGT+AGG	-	chr5.4:21461665-21461684	MS.gene070879:CDS	30.0%
!!	TATCGATTCGTGATTGATAT+TGG	-	chr5.4:21462625-21462644	MS.gene070879:CDS	30.0%
	AAGGAAGCAATAGAACACTT+AGG	-	chr5.4:21462064-21462083	MS.gene070879:CDS	35.0%
	AATGGAAAGCTTGTAATGGT+TGG	-	chr5.4:21462293-21462312	MS.gene070879:CDS	35.0%
	ACAACTCCTATATTCATACC+AGG	+	chr5.4:21461953-21461972	None:intergenic	35.0%
	ATAGCTGGAAGTTTAATTGG+AGG	-	chr5.4:21462478-21462497	MS.gene070879:intron	35.0%
	ATTAATACCTGCATCTGATC+TGG	+	chr5.4:21462118-21462137	None:intergenic	35.0%
	CACATTATGCAGAATGAATG+GGG	-	chr5.4:21461351-21461370	MS.gene070879:CDS	35.0%
	CCTCCAAGTAAAGAAAATAC+AGG	+	chr5.4:21462344-21462363	None:intergenic	35.0%
	GGTATGAATATAGGAGTTGT+CGG	-	chr5.4:21461953-21461972	MS.gene070879:CDS	35.0%
	TAAAGTTGGAGACAAAGTAG+GGG	-	chr5.4:21461667-21461686	MS.gene070879:CDS	35.0%
	TAAATACAGGCACGAACTTA+CGG	-	chr5.4:21461607-21461626	MS.gene070879:CDS	35.0%
	TAGCTGGAAGTTTAATTGGA+GGG	-	chr5.4:21462479-21462498	MS.gene070879:intron	35.0%
	TGAGAAAGATGTAGCATTCA+AGG	-	chr5.4:21461296-21461315	MS.gene070879:CDS	35.0%
!	CATCCTGTTTTACCATTGAT+TGG	-	chr5.4:21462257-21462276	MS.gene070879:CDS	35.0%
!	CCTGTATTTTCTTTACTTGG+AGG	-	chr5.4:21462341-21462360	MS.gene070879:intron	35.0%
!	TATAGTCCTCTTCGACATTT+TGG	-	chr5.4:21461917-21461936	MS.gene070879:CDS	35.0%
!!	CATTCAAGGTGTTGTATTGT+GGG	-	chr5.4:21461310-21461329	MS.gene070879:CDS	35.0%
	AAATACAGGCACGAACTTAC+GGG	-	chr5.4:21461608-21461627	MS.gene070879:CDS	40.0%
	AAGGAGTTGAAAACATACCC+CGG	+	chr5.4:21461399-21461418	None:intergenic	40.0%
	AATATAGGAGTTGTCGGTCT+TGG	-	chr5.4:21461959-21461978	MS.gene070879:CDS	40.0%
	ATAGGAACAACCTCAATCTC+AGG	+	chr5.4:21462556-21462575	None:intergenic	40.0%
	CAATGTAACTCCTGAGATTG+AGG	-	chr5.4:21462543-21462562	MS.gene070879:CDS	40.0%
	CACTTTAGCACCAAAAGCTT+TGG	+	chr5.4:21462014-21462033	None:intergenic	40.0%
	CGATTATGTCAACACAGCAA+TGG	-	chr5.4:21462576-21462595	MS.gene070879:CDS	40.0%
	GACAAGCCTGGTATGAATAT+AGG	-	chr5.4:21461944-21461963	MS.gene070879:CDS	40.0%
	TCAAGTCCAAAATGTCGAAG+AGG	+	chr5.4:21461926-21461945	None:intergenic	40.0%
!	GAAAATACAGGAAGCTCTAG+AGG	+	chr5.4:21462332-21462351	None:intergenic	40.0%
!	GCTGTGTTGACATAATCGAT+AGG	+	chr5.4:21462574-21462593	None:intergenic	40.0%
!	GTGAAATTCGCCAAAGCTTT+TGG	-	chr5.4:21462001-21462020	MS.gene070879:CDS	40.0%
!	TTCAATGGTAAGTATCGCGA+TGG	-	chr5.4:21461773-21461792	MS.gene070879:intron	40.0%
!!	GCATTCAAGGTGTTGTATTG+TGG	-	chr5.4:21461309-21461328	MS.gene070879:CDS	40.0%
	AAAACATACCCCGGGACTAA+TGG	+	chr5.4:21461390-21461409	None:intergenic	45.0%
	AACCACCTATCCATTAGTCC+CGG	-	chr5.4:21461377-21461396	MS.gene070879:CDS	45.0%
	ACTTACGGGTGTAGTAACAG+AGG	-	chr5.4:21461622-21461641	MS.gene070879:CDS	45.0%
	AGGAGTTGAAAACATACCCC+GGG	+	chr5.4:21461398-21461417	None:intergenic	45.0%
	CATCAAGACTTCAGGGAAAC+AGG	-	chr5.4:21461273-21461292	MS.gene070879:CDS	45.0%
	CCATCAAGAGGTAAGCTATC+AGG	+	chr5.4:21461863-21461882	None:intergenic	45.0%
	CCTGATAGCTTACCTCTTGA+TGG	-	chr5.4:21461860-21461879	MS.gene070879:CDS	45.0%
	CTCATCTGCAACCATTGAGT+CGG	+	chr5.4:21461822-21461841	None:intergenic	45.0%
	GCTGCAACGAGTACTTTGAA+TGG	-	chr5.4:21462209-21462228	MS.gene070879:CDS	45.0%
	GTAGGGAGAAAGTCAATAGC+TGG	-	chr5.4:21462463-21462482	MS.gene070879:intron	45.0%
!	CATTTTGGACTTGACAAGCC+TGG	-	chr5.4:21461932-21461951	MS.gene070879:CDS	45.0%
!!	TCTTGGTGGACTTGGTCATA+TGG	-	chr5.4:21461976-21461995	MS.gene070879:CDS	45.0%
	ACCACCTATCCATTAGTCCC+GGG	-	chr5.4:21461378-21461397	MS.gene070879:CDS	50.0%
	ACGGGTGTAGTAACAGAGGT+GGG	-	chr5.4:21461626-21461645	MS.gene070879:CDS	50.0%
	AGTTGGAGACAAAGTAGGGG+TGG	-	chr5.4:21461670-21461689	MS.gene070879:CDS	50.0%
	ATAGGAGTTGTCGGTCTTGG+TGG	-	chr5.4:21461962-21461981	MS.gene070879:CDS	50.0%
	CAAAGTAGGGGTGGGATACA+TGG	-	chr5.4:21461679-21461698	MS.gene070879:CDS	50.0%
	GGAGAAACCTCCATAGGTGA+TGG	+	chr5.4:21461801-21461820	None:intergenic	50.0%
	GTTGGAGACAAAGTAGGGGT+GGG	-	chr5.4:21461671-21461690	MS.gene070879:CDS	50.0%
	TACGGGTGTAGTAACAGAGG+TGG	-	chr5.4:21461625-21461644	MS.gene070879:CDS	50.0%
	TGAGTCGGAGAAACCTCCAT+AGG	+	chr5.4:21461807-21461826	None:intergenic	50.0%
	TGGAGGTTTCTCCGACTCAA+TGG	-	chr5.4:21461808-21461827	MS.gene070879:intron	50.0%
!	ATACCCCGGGACTAATGGAT+AGG	+	chr5.4:21461385-21461404	None:intergenic	50.0%
!	GCAATGGAGCGTCTTGCTAA+AGG	-	chr5.4:21462592-21462611	MS.gene070879:CDS	50.0%
	CCACCTATCCATTAGTCCCG+GGG	-	chr5.4:21461379-21461398	MS.gene070879:CDS	55.0%
	CCTGCATCTGATCTGGATCG+CGG	+	chr5.4:21462111-21462130	None:intergenic	55.0%
	GATGGCACCATCACCTATGG+AGG	-	chr5.4:21461791-21461810	MS.gene070879:intron	55.0%
	GTGACTCCAGCGCAAAGAAG+AGG	+	chr5.4:21461893-21461912	None:intergenic	55.0%
	TCCAGCGCAAAGAAGAGGAC+CGG	+	chr5.4:21461888-21461907	None:intergenic	55.0%
!!	AGCTTACCTCTTGATGGCGC+CGG	-	chr5.4:21461866-21461885	MS.gene070879:CDS	55.0%
!!	GTTGTCGGTCTTGGTGGACT+TGG	-	chr5.4:21461968-21461987	MS.gene070879:CDS	55.0%
	CCGCGATCCAGATCAGATGC+AGG	-	chr5.4:21462108-21462127	MS.gene070879:CDS	60.0%
	CGCGATGGCACCATCACCTA+TGG	-	chr5.4:21461788-21461807	MS.gene070879:intron	60.0%
!	CCCCGGGACTAATGGATAGG+TGG	+	chr5.4:21461382-21461401	None:intergenic	60.0%
	AGAGGACCGGCGCCATCAAG+AGG	+	chr5.4:21461875-21461894	None:intergenic	65.0%
	GCCGGTCCTCTTCTTTGCGC+TGG	-	chr5.4:21461884-21461903	MS.gene070879:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	21461267	21462672	21461267	ID=MS.gene070879
chr5.4	mRNA	21461267	21462672	21461267	ID=MS.gene070879.t1;Parent=MS.gene070879
chr5.4	exon	21462539	21462672	21462539	ID=MS.gene070879.t1.exon1;Parent=MS.gene070879.t1
chr5.4	CDS	21462539	21462672	21462539	ID=cds.MS.gene070879.t1;Parent=MS.gene070879.t1
chr5.4	exon	21461810	21462323	21461810	ID=MS.gene070879.t1.exon2;Parent=MS.gene070879.t1
chr5.4	CDS	21461810	21462323	21461810	ID=cds.MS.gene070879.t1;Parent=MS.gene070879.t1
chr5.4	exon	21461577	21461730	21461577	ID=MS.gene070879.t1.exon3;Parent=MS.gene070879.t1
chr5.4	CDS	21461577	21461730	21461577	ID=cds.MS.gene070879.t1;Parent=MS.gene070879.t1
chr5.4	exon	21461267	21461472	21461267	ID=MS.gene070879.t1.exon4;Parent=MS.gene070879.t1
chr5.4	CDS	21461267	21461472	21461267	ID=cds.MS.gene070879.t1;Parent=MS.gene070879.t1

Gene Sequence

>MS.gene070879

ATGGTGCATCAAGACTTCAGGGAAACAGGTGAGAAAGATGTAGCATTCAAGGTGTTGTATTGTGGGATATGTCACTCTGACCTTCACATTATGCAGAATGAATGGGGCAAAACCACCTATCCATTAGTCCCGGGGTATGTTTTCAACTCCTTTAACTTCTATTATAGTTTATTGCGCCGTTTCTTTTTAATTGTCACGTTTTAAGTAAAACAAATATTTTTTTATAAGTTAAACCCTTACTTTATCAATTATTTTATACTTAATATGATAACTAGTAGAGTGAGTCAATCAAAAGAAACGAGTAGAAAATAATTGCTGAATTTGGTTATAACTGATGTAATAAATACAGGCACGAACTTACGGGTGTAGTAACAGAGGTGGGAAGCAAAGTAAAAAAGTTTAAAGTTGGAGACAAAGTAGGGGTGGGATACATGGTTGATTCATGTCGTTCATGCGAAAATTGTGCTGAAGATCTTGAGAATTACTGCCCCAAATATACTCAAACATTCAATGGTAAGTATCGCGATGGCACCATCACCTATGGAGGTTTCTCCGACTCAATGGTTGCAGATGAGCACTTTGTGATTCGCATTCCTGATAGCTTACCTCTTGATGGCGCCGGTCCTCTTCTTTGCGCTGGAGTCACAGTGTATAGTCCTCTTCGACATTTTGGACTTGACAAGCCTGGTATGAATATAGGAGTTGTCGGTCTTGGTGGACTTGGTCATATGGCTGTGAAATTCGCCAAAGCTTTTGGTGCTAAAGTGACCGTAATTAGTACATCACCTAAAAAGGAAAAGGAAGCAATAGAACACTTAGGAGCAGACTCGTTTCTTGTAAGCCGCGATCCAGATCAGATGCAGGTATTAATTTCGGAATTTTTTGTTATTTCAGAAATTGATAATGTCAATCAAGAAACTAATATTGTACAACATTTCATAGGCTGCAACGAGTACTTTGAATGGCATTATTGACACAGTTTCAGTGAGTCATCCTGTTTTACCATTGATTGGTTTATTGAAATCTAATGGAAAGCTTGTAATGGTTGGTGCAGTAGCAAAGCCTCTAGAGCTTCCTGTATTTTCTTTACTTGGAGGTAAGCATATGAAAAATGCTTGCATATATACACACTCACAAAACGCTAATGCTAATTATGCAGAACTTTAATGCTCACTTGTATTAATTTCGCATTATTGGTAGGGAGAAAGTCAATAGCTGGAAGTTTAATTGGAGGGATAAAGGAGACACAAGAAATGATTGATTTTGCAGCTAAACACAATGTAACTCCTGAGATTGAGGTTGTTCCTATCGATTATGTCAACACAGCAATGGAGCGTCTTGCTAAAGGAGATGTCAAATATCGATTCGTGATTGATATTGGAAATTCATTAAAACCAAGTCCTTAA

Protein sequence

>MS.gene070879.t1

MVHQDFRETGEKDVAFKVLYCGICHSDLHIMQNEWGKTTYPLVPGHELTGVVTEVGSKVKKFKVGDKVGVGYMVDSCRSCENCAEDLENYCPKYTQTFNGKYRDGTITYGGFSDSMVADEHFVIRIPDSLPLDGAGPLLCAGVTVYSPLRHFGLDKPGMNIGVVGLGGLGHMAVKFAKAFGAKVTVISTSPKKEKEAIEHLGADSFLVSRDPDQMQAATSTLNGIIDTVSVSHPVLPLIGLLKSNGKLVMVGAVAKPLELPVFSLLGGRKSIAGSLIGGIKETQEMIDFAAKHNVTPEIEVVPIDYVNTAMERLAKGDVKYRFVIDIGNSLKPSP