AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene068613

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068613.t1 MTR_5g031310 95.856 362 15 0 1 362 1 362 0.0 694
MS.gene068613.t1 MTR_5g031305 93.370 362 24 0 1 362 1 362 0.0 682
MS.gene068613.t1 MTR_5g031300 84.211 361 57 0 1 361 1 361 0.0 625
MS.gene068613.t1 MTR_5g031360 86.592 358 48 0 5 362 6 363 0.0 624
MS.gene068613.t1 MTR_5g031230 82.548 361 62 1 1 361 1 360 0.0 597
MS.gene068613.t1 MTR_4g115300 82.203 354 63 0 8 361 7 360 0.0 591
MS.gene068613.t1 MTR_5g031390 82.486 354 62 0 8 361 7 360 0.0 587
MS.gene068613.t1 MTR_5g031250 80.055 361 71 1 1 361 1 360 0.0 576
MS.gene068613.t1 MTR_5g031210 79.379 354 73 0 8 361 7 360 0.0 562
MS.gene068613.t1 MTR_5g031190 75.637 353 86 0 9 361 8 360 0.0 549
MS.gene068613.t1 MTR_5g031180 79.944 354 71 0 8 361 7 360 0.0 546
MS.gene068613.t1 MTR_8g068190 78.814 354 75 0 8 361 7 360 0.0 540
MS.gene068613.t1 MTR_8g092630 61.050 362 135 2 2 358 53 413 2.27e-149 428
MS.gene068613.t1 MTR_5g031430 59.710 345 139 0 15 359 14 358 6.35e-143 409
MS.gene068613.t1 MTR_5g031250 81.405 242 44 1 1 242 1 241 2.97e-138 394
MS.gene068613.t1 MTR_1g025950 56.897 348 149 1 10 356 8 355 1.08e-131 381
MS.gene068613.t1 MTR_1g107425 51.020 343 168 0 12 354 9 351 3.02e-121 354
MS.gene068613.t1 MTR_2g009810 53.602 347 157 3 15 359 62 406 7.95e-117 345
MS.gene068613.t1 MTR_1g107425 51.211 289 140 1 12 299 9 297 3.34e-96 288
MS.gene068613.t1 MTR_2g009820 52.155 232 108 2 131 359 23 254 5.49e-64 204
MS.gene068613.t1 MTR_5g031290 76.522 115 27 0 248 362 80 194 6.96e-55 179
MS.gene068613.t1 MTR_5g031290 82.716 81 12 2 1 80 1 80 7.19e-41 142
MS.gene068613.t1 MTR_8g095220 26.599 297 186 8 11 279 51 343 1.95e-21 95.1
MS.gene068613.t1 MTR_8g095220 28.125 256 157 7 11 243 51 302 3.59e-21 93.2
MS.gene068613.t1 MTR_0001s0660 28.852 305 187 12 39 319 35 333 6.60e-21 94.0
MS.gene068613.t1 MTR_6g465010 27.830 212 139 5 26 234 13 213 1.77e-16 80.1
MS.gene068613.t1 MTR_6g466320 28.638 213 136 6 26 234 29 229 2.40e-16 79.7
MS.gene068613.t1 MTR_6g465010 28.638 213 136 6 26 234 29 229 2.40e-16 79.7
MS.gene068613.t1 MTR_3g089977 24.339 378 242 13 12 355 12 379 1.58e-15 77.4
MS.gene068613.t1 MTR_3g089940 24.728 368 234 12 12 345 12 370 2.69e-15 76.6
MS.gene068613.t1 MTR_3g089940 24.274 379 244 12 1 345 1 370 5.39e-15 75.9
MS.gene068613.t1 MTR_5g045710 24.405 336 221 11 39 345 35 366 1.92e-14 74.3
MS.gene068613.t1 MTR_3g089940 25.856 263 168 7 12 254 12 267 2.77e-14 73.6
MS.gene068613.t1 MTR_3g089940 25.182 274 178 7 1 254 1 267 4.80e-14 72.8
MS.gene068613.t1 MTR_3g089970 24.317 366 234 12 14 345 15 371 6.99e-14 72.4
MS.gene068613.t1 MTR_7g068320 26.377 345 200 13 40 341 48 381 9.21e-14 72.4
MS.gene068613.t1 MTR_7g068330 26.087 345 200 14 40 341 41 373 1.66e-12 68.6
MS.gene068613.t1 MTR_1g069430 26.471 340 214 13 1 313 1 331 4.35e-12 67.0
MS.gene068613.t1 MTR_7g099040 24.930 357 225 13 2 328 4 347 1.36e-11 65.5
MS.gene068613.t1 MTR_5g031380 64.103 39 14 0 81 119 19 57 2.25e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068613.t1 AT4G37980 71.709 357 99 1 6 362 3 357 1.61e-175 492
MS.gene068613.t1 AT4G37990 70.868 357 102 1 6 362 3 357 1.83e-171 482
MS.gene068613.t1 AT4G37970 63.173 353 130 0 9 361 9 361 1.91e-163 461
MS.gene068613.t1 AT4G39330 62.500 352 132 0 9 360 8 359 2.03e-162 459
MS.gene068613.t1 AT2G21730 60.571 350 136 2 13 360 6 355 4.68e-150 428
MS.gene068613.t1 AT2G21890 59.885 349 139 1 13 360 6 354 6.07e-150 428
MS.gene068613.t1 AT4G37980 70.470 298 86 1 6 303 3 298 3.92e-135 387
MS.gene068613.t1 AT4G39330 62.162 296 112 0 9 304 8 303 3.06e-131 378
MS.gene068613.t1 AT4G37970 61.724 290 111 0 9 298 9 298 8.84e-127 366
MS.gene068613.t1 AT3G19450 52.312 346 165 0 12 357 9 354 5.69e-115 338
MS.gene068613.t1 AT1G72680 50.437 343 169 1 17 359 14 355 1.36e-113 335
MS.gene068613.t1 AT4G34230 50.575 348 172 0 7 354 3 350 1.01e-106 317
MS.gene068613.t1 AT4G34230 50.287 348 173 0 7 354 3 350 9.29e-106 315
MS.gene068613.t1 AT4G37970 60.924 238 93 0 9 246 9 246 5.98e-101 299
MS.gene068613.t1 AT4G37970 61.277 235 91 0 9 243 9 243 4.53e-100 296
MS.gene068613.t1 AT5G63620 26.207 290 186 6 16 279 58 345 4.09e-23 100
MS.gene068613.t1 AT5G63620 26.207 290 186 6 16 279 58 345 5.95e-23 99.8
MS.gene068613.t1 AT5G63620 27.711 249 157 5 16 243 58 304 2.60e-22 96.3
MS.gene068613.t1 AT1G22430 26.183 317 202 12 40 328 42 354 3.47e-17 82.4
MS.gene068613.t1 AT1G22430 26.183 317 202 12 40 328 42 354 3.47e-17 82.4
MS.gene068613.t1 AT5G43940 26.648 349 216 12 1 320 1 338 2.46e-16 80.1
MS.gene068613.t1 AT5G43940 26.774 310 191 9 40 320 48 350 7.29e-16 78.6
MS.gene068613.t1 AT5G51970 25.490 255 169 6 37 279 40 285 8.24e-16 78.2
MS.gene068613.t1 AT5G51970 25.490 255 169 6 37 279 40 285 8.24e-16 78.2
MS.gene068613.t1 AT1G32780 26.316 361 221 13 40 359 37 393 4.35e-15 76.3
MS.gene068613.t1 AT4G22110 25.373 335 214 13 40 344 43 371 3.55e-14 73.6
MS.gene068613.t1 AT4G22110 25.373 335 214 13 40 344 43 371 3.55e-14 73.6
MS.gene068613.t1 AT1G22430 26.000 200 129 5 40 222 42 239 4.39e-13 69.7
MS.gene068613.t1 AT1G22430 26.000 200 129 5 40 222 42 239 6.35e-13 68.9
MS.gene068613.t1 AT1G22430 26.000 200 129 5 40 222 42 239 6.35e-13 68.9
MS.gene068613.t1 AT1G77120 22.597 385 261 13 1 355 1 378 2.56e-12 67.8
MS.gene068613.t1 AT5G42250 21.408 341 235 10 40 353 45 379 1.51e-11 65.5

Find 91 sgRNAs with CRISPR-Local

Find 113 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAGTAGCTGGAAGTTTAAT+TGG 0.186001 5.1:-21576606 MS.gene068613:CDS
TATAGTCCTCTTCGACATTT+TGG 0.197168 5.1:-21577190 MS.gene068613:CDS
AAACCAATCAATGGTAAAAT+AGG 0.229602 5.1:+21576834 None:intergenic
TATCGATTCGTGATTGATAT+TGG 0.245698 5.1:-21576456 MS.gene068613:CDS
GTGAAATTCGCCAAAGCTTT+TGG 0.268679 5.1:-21577106 MS.gene068613:CDS
ATTGGTTTATTGAAATCTAA+TGG 0.271827 5.1:-21576819 MS.gene068613:CDS
TCCTGCAATGTTTAAGGGCT+TGG 0.287864 5.1:+21576420 None:intergenic
TCTTGGTGGACTTGGTCATA+TGG 0.309264 5.1:-21577131 MS.gene068613:CDS
ACCACATGTGACTGTTTGTT+GGG 0.314826 5.1:+21577351 None:intergenic
CATCAAAACTTTAGGGAAAC+TGG 0.347590 5.1:-21577779 MS.gene068613:intron
GCCCAACCAAAGGCCTTCTT+AGG 0.362894 5.1:+21578075 None:intergenic
CACCACATGTGACTGTTTGT+TGG 0.363970 5.1:+21577350 None:intergenic
CCCTGGAACTAATGGATAGA+TGG 0.366233 5.1:+21577673 None:intergenic
AAGTTTAATTGGAGGGATAA+AGG 0.373525 5.1:-21576595 MS.gene068613:CDS
GTTGTTGGTCTTGGTGGACT+TGG 0.377317 5.1:-21577139 MS.gene068613:CDS
GTTACGAGATGTGCTAATTA+CGG 0.380792 5.1:+21577072 None:intergenic
ATAGGAATAACCTCAATATC+AGG 0.384692 5.1:+21576528 None:intergenic
TCCATCTATCCATTAGTTCC+AGG 0.385414 5.1:-21577674 MS.gene068613:CDS
AACTTACCTCTTGATGTTGC+CGG 0.388597 5.1:-21577241 MS.gene068613:CDS
TCCTAAGAAGGCCTTTGGTT+GGG 0.390941 5.1:-21578076 MS.gene068613:CDS
ACATCAAGAGGTAAGTTATC+AGG 0.391560 5.1:+21577247 None:intergenic
CGTGATGGCACCGTCACATA+TGG 0.395744 5.1:-21577319 MS.gene068613:CDS
GAGCATCCTAAGAAGGCCTT+TGG 0.400649 5.1:-21578081 MS.gene068613:CDS
ATAGGTGTTGTTGGTCTTGG+TGG 0.407624 5.1:-21577145 MS.gene068613:CDS
GCCGGTCCTCTTCTTTGCGC+TGG 0.408424 5.1:-21577223 MS.gene068613:CDS
CACATTAGCACCAAAAGCTT+TGG 0.409643 5.1:+21577096 None:intergenic
CCCCTTTCAATTTCTCCAGA+AGG 0.415467 5.1:-21578026 MS.gene068613:intron
AATATAGGTGTTGTTGGTCT+TGG 0.416186 5.1:-21577148 MS.gene068613:CDS
TCAAATACCTGCATCTGATC+TGG 0.416400 5.1:+21576992 None:intergenic
AAATGCAGGCATGAACTTGC+GGG 0.418681 5.1:-21577499 MS.gene068613:intron
GAAGCATACCCTGGAACTAA+TGG 0.426302 5.1:+21577665 None:intergenic
AAAGTGGAAAAGTTTAAAGT+TGG 0.429905 5.1:-21577454 MS.gene068613:CDS
TTCATATGATGAAGAATGAA+TGG 0.431554 5.1:-21577703 MS.gene068613:CDS
GTAGGGAGAAAGTTAGTAGC+TGG 0.438913 5.1:-21576618 MS.gene068613:intron
CATTCAAGGTGTTGTATTGT+GGG 0.442854 5.1:-21577742 MS.gene068613:CDS
TTTAAAGTTGGAGACAAAGT+AGG 0.443860 5.1:-21577442 MS.gene068613:CDS
TCATATGATGAAGAATGAAT+GGG 0.446572 5.1:-21577702 MS.gene068613:CDS
GACGGTGCCATCACGATACT+TGG 0.453032 5.1:+21577327 None:intergenic
TGGGCAGCTAGAGATCCTTC+TGG 0.459135 5.1:-21578057 MS.gene068613:CDS
TGAGAAAGATGTAGCATTCA+AGG 0.467351 5.1:-21577756 MS.gene068613:CDS
GACAAACCTGGTATGAATAT+AGG 0.467866 5.1:-21577163 MS.gene068613:CDS
GCTGTGTTGACATAATCAAT+AGG 0.471251 5.1:+21576510 None:intergenic
CAAAGTAGGTGTGGGATACA+TGG 0.478011 5.1:-21577428 MS.gene068613:CDS
CACATCTCGTAACAAAGAAA+AGG 0.483022 5.1:-21577062 MS.gene068613:CDS
TTCCACTTTGCTTCCTACCT+CGG 0.483575 5.1:+21577468 None:intergenic
CCTCCAAGTAAAGAAAATAC+AGG 0.487127 5.1:+21576753 None:intergenic
GATTTCAATAAACCAATCAA+TGG 0.487235 5.1:+21576825 None:intergenic
CAATGTAAAGCCTGATATTG+AGG 0.489359 5.1:-21576538 MS.gene068613:CDS
TGGAGGTTATTCTGACTCAA+TGG 0.494148 5.1:-21577299 MS.gene068613:CDS
ATTCATTCTTCATCATATGA+AGG 0.495059 5.1:+21577705 None:intergenic
ACCTTCTGGAGAAATTGAAA+GGG 0.500807 5.1:+21578025 None:intergenic
ATCCTAAGAAGGCCTTTGGT+TGG 0.502470 5.1:-21578077 MS.gene068613:CDS
AACCTTCTGGAGAAATTGAA+AGG 0.504412 5.1:+21578024 None:intergenic
GGTATGAATATAGGTGTTGT+TGG 0.507632 5.1:-21577154 MS.gene068613:CDS
AAAGGAAGCAATAGAACACT+TGG 0.509890 5.1:-21577044 MS.gene068613:CDS
GCATTCAAGGTGTTGTATTG+TGG 0.522756 5.1:-21577743 MS.gene068613:CDS
AGTGTTAGAGCATCCTAAGA+AGG 0.523098 5.1:-21578088 MS.gene068613:CDS
GCTGCAACAGGTACTTTGAA+TGG 0.526500 5.1:-21576885 MS.gene068613:CDS
TAGCTGGAAGTTTAATTGGA+GGG 0.528518 5.1:-21576602 MS.gene068613:CDS
AGTTGGAGACAAAGTAGGTG+TGG 0.528716 5.1:-21577437 MS.gene068613:CDS
CCACATGTGACTGTTTGTTG+GGG 0.540150 5.1:+21577352 None:intergenic
GAAAATACAGGAAGCTCTAG+AGG 0.546279 5.1:+21576765 None:intergenic
CCATCTATCCATTAGTTCCA+GGG 0.551121 5.1:-21577673 MS.gene068613:intron
GTAGCTGGAAGTTTAATTGG+AGG 0.559892 5.1:-21576603 MS.gene068613:CDS
GGAAAGCTTGTAATGGTTGG+TGG 0.563386 5.1:-21576798 MS.gene068613:CDS
TCCAGCGCAAAGAAGAGGAC+CGG 0.564917 5.1:+21577222 None:intergenic
GTGACTCCAGCGCAAAGAAG+AGG 0.566494 5.1:+21577217 None:intergenic
TAAATGCAGGCATGAACTTG+CGG 0.570766 5.1:-21577500 MS.gene068613:intron
ATCTCTAGCTGCCCAACCAA+AGG 0.576625 5.1:+21578065 None:intergenic
GTTGGAGACAAAGTAGGTGT+GGG 0.583586 5.1:-21577436 MS.gene068613:CDS
ATCTAATGGAAAGCTTGTAA+TGG 0.587781 5.1:-21576805 MS.gene068613:CDS
CTGCGATCCAGATCAGATGC+AGG 0.590600 5.1:-21576999 MS.gene068613:intron
GCTTGTAATGGTTGGTGGAG+TGG 0.601696 5.1:-21576793 MS.gene068613:CDS
AGAGGACCGGCAACATCAAG+AGG 0.610980 5.1:+21577235 None:intergenic
AATGGAAAGCTTGTAATGGT+TGG 0.618001 5.1:-21576801 MS.gene068613:CDS
ACAACACCTATATTCATACC+AGG 0.624332 5.1:+21577157 None:intergenic
TTTGAATGGTATTATTGACA+CGG 0.632246 5.1:-21576871 MS.gene068613:CDS
AAAGGGGAGAGAACACCAGA+AGG 0.635132 5.1:+21578042 None:intergenic
GCGGGTATAGTAACCGAGGT+AGG 0.635211 5.1:-21577481 MS.gene068613:CDS
TGATTATGTCAACACAGCAG+TGG 0.636651 5.1:-21576505 MS.gene068613:CDS
TCGAGTCCAAAATGTCGAAG+AGG 0.641706 5.1:+21577184 None:intergenic
GATGGCACCGTCACATATGG+AGG 0.644175 5.1:-21577316 MS.gene068613:CDS
CCTTCTGGAGAAATTGAAAG+GGG 0.647504 5.1:+21578026 None:intergenic
AGAATAACCTCCATATGTGA+CGG 0.659885 5.1:+21577309 None:intergenic
CACATGTGACTGTTTGTTGG+GGG 0.661508 5.1:+21577353 None:intergenic
AACCGAGGTAGGAAGCAAAG+TGG 0.665272 5.1:-21577470 MS.gene068613:CDS
CATATGATGAAGAATGAATG+GGG 0.667164 5.1:-21577701 MS.gene068613:CDS
AAGGAAGCAATAGAACACTT+GGG 0.667331 5.1:-21577043 MS.gene068613:CDS
CCCCAACAAACAGTCACATG+TGG 0.678792 5.1:-21577352 MS.gene068613:CDS
TGTGGTGCCAAGTATCGTGA+TGG 0.704207 5.1:-21577334 MS.gene068613:CDS
ACTTGCGGGTATAGTAACCG+AGG 0.756724 5.1:-21577485 MS.gene068613:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGTAAGTATAAAGATATATA+TGG + chr5.1:21576942-21576961 None:intergenic 15.0%
!! ATAAATTTCACATTATTTGT+AGG - chr5.1:21577900-21577919 MS.gene068613:intron 15.0%
!! TAAATTTCACATTATTTGTA+GGG - chr5.1:21577901-21577920 MS.gene068613:intron 15.0%
!! TAAAGTATAATTGTAGTAGA+AGG + chr5.1:21576902-21576921 None:intergenic 20.0%
!! TGCTTATAATTAATAAATGC+AGG - chr5.1:21577023-21577042 MS.gene068613:CDS 20.0%
!!! ATAATGTACCACATTTTAAT+AGG - chr5.1:21577629-21577648 MS.gene068613:intron 20.0%
!!! ATCAACTCATCTTTATTTAA+TGG - chr5.1:21576547-21576566 MS.gene068613:CDS 20.0%
!!! ATTGGTTTATTGAAATCTAA+TGG - chr5.1:21577717-21577736 MS.gene068613:CDS 20.0%
! AAACCAATCAATGGTAAAAT+AGG + chr5.1:21577705-21577724 None:intergenic 25.0%
! ATTCATTCTTCATCATATGA+AGG + chr5.1:21576834-21576853 None:intergenic 25.0%
! GATTTCAATAAACCAATCAA+TGG + chr5.1:21577714-21577733 None:intergenic 25.0%
! TCATATGATGAAGAATGAAT+GGG - chr5.1:21576834-21576853 MS.gene068613:CDS 25.0%
! TTATAATGAAGTAAACCTTC+TGG + chr5.1:21576528-21576547 None:intergenic 25.0%
! TTCATATGATGAAGAATGAA+TGG - chr5.1:21576833-21576852 MS.gene068613:CDS 25.0%
! TTTGAATGGTATTATTGACA+CGG - chr5.1:21577665-21577684 MS.gene068613:intron 25.0%
!! AAAGTGGAAAAGTTTAAAGT+TGG - chr5.1:21577082-21577101 MS.gene068613:CDS 25.0%
!!! TCAAGAAATGATTGATTTTG+CGG - chr5.1:21577968-21577987 MS.gene068613:intron 25.0%
AAGTTTAATTGGAGGGATAA+AGG - chr5.1:21577941-21577960 MS.gene068613:intron 30.0%
ATAATTGTAGTAGAAGGAGT+TGG + chr5.1:21576896-21576915 None:intergenic 30.0%
ATAGGAATAACCTCAATATC+AGG + chr5.1:21578011-21578030 None:intergenic 30.0%
ATCTAATGGAAAGCTTGTAA+TGG - chr5.1:21577731-21577750 MS.gene068613:CDS 30.0%
CATATGATGAAGAATGAATG+GGG - chr5.1:21576835-21576854 MS.gene068613:CDS 30.0%
CCTACTAATTAAGTCAACTT+TGG - chr5.1:21576732-21576751 MS.gene068613:intron 30.0%
TACTTTATTCCTACGTTACT+TGG - chr5.1:21576915-21576934 MS.gene068613:intron 30.0%
TTAGTAGCTGGAAGTTTAAT+TGG - chr5.1:21577930-21577949 MS.gene068613:intron 30.0%
! CATCCTATTTTACCATTGAT+TGG - chr5.1:21577699-21577718 MS.gene068613:CDS 30.0%
! CCAAAGTTGACTTAATTAGT+AGG + chr5.1:21576735-21576754 None:intergenic 30.0%
! CTTCCTGTATTTTCTTTACT+TGG - chr5.1:21577780-21577799 MS.gene068613:intron 30.0%
! TTTAAAGTTGGAGACAAAGT+AGG - chr5.1:21577094-21577113 MS.gene068613:CDS 30.0%
! TTTGGTGCATCAAAACTTTA+GGG - chr5.1:21576750-21576769 MS.gene068613:intron 30.0%
!! TATCGATTCGTGATTGATAT+TGG - chr5.1:21578080-21578099 MS.gene068613:CDS 30.0%
AAAGGAAGCAATAGAACACT+TGG - chr5.1:21577492-21577511 MS.gene068613:CDS 35.0%
AAGGAAGCAATAGAACACTT+GGG - chr5.1:21577493-21577512 MS.gene068613:CDS 35.0%
AATGGAAAGCTTGTAATGGT+TGG - chr5.1:21577735-21577754 MS.gene068613:CDS 35.0%
ACAACACCTATATTCATACC+AGG + chr5.1:21577382-21577401 None:intergenic 35.0%
ACATCAAGAGGTAAGTTATC+AGG + chr5.1:21577292-21577311 None:intergenic 35.0%
AGAATAACCTCCATATGTGA+CGG + chr5.1:21577230-21577249 None:intergenic 35.0%
ATATATGGTCCAAGTAACGT+AGG + chr5.1:21576927-21576946 None:intergenic 35.0%
CAATGTAAAGCCTGATATTG+AGG - chr5.1:21577998-21578017 MS.gene068613:intron 35.0%
CACATCTCGTAACAAAGAAA+AGG - chr5.1:21577474-21577493 MS.gene068613:CDS 35.0%
CCTCCAAGTAAAGAAAATAC+AGG + chr5.1:21577786-21577805 None:intergenic 35.0%
GACAAACCTGGTATGAATAT+AGG - chr5.1:21577373-21577392 MS.gene068613:CDS 35.0%
GTTACGAGATGTGCTAATTA+CGG + chr5.1:21577467-21577486 None:intergenic 35.0%
TAGCTGGAAGTTTAATTGGA+GGG - chr5.1:21577934-21577953 MS.gene068613:intron 35.0%
TGAGAAAGATGTAGCATTCA+AGG - chr5.1:21576780-21576799 MS.gene068613:CDS 35.0%
TGTTGCAGCCTATTAAAATG+TGG + chr5.1:21577640-21577659 None:intergenic 35.0%
! AACCTTCTGGAGAAATTGAA+AGG + chr5.1:21576515-21576534 None:intergenic 35.0%
! ACCTTCTGGAGAAATTGAAA+GGG + chr5.1:21576514-21576533 None:intergenic 35.0%
! CATCAAAACTTTAGGGAAAC+TGG - chr5.1:21576757-21576776 MS.gene068613:CDS 35.0%
! CCTGTATTTTCTTTACTTGG+AGG - chr5.1:21577783-21577802 MS.gene068613:intron 35.0%
! CTTTGGTGCATCAAAACTTT+AGG - chr5.1:21576749-21576768 MS.gene068613:intron 35.0%
! GCTGTGTTGACATAATCAAT+AGG + chr5.1:21578029-21578048 None:intergenic 35.0%
! GGTATGAATATAGGTGTTGT+TGG - chr5.1:21577382-21577401 MS.gene068613:CDS 35.0%
! TATAGTCCTCTTCGACATTT+TGG - chr5.1:21577346-21577365 MS.gene068613:CDS 35.0%
!! AATATAGGTGTTGTTGGTCT+TGG - chr5.1:21577388-21577407 MS.gene068613:CDS 35.0%
!! ACATTTTAATAGGCTGCAAC+AGG - chr5.1:21577639-21577658 MS.gene068613:intron 35.0%
!! CATTCAAGGTGTTGTATTGT+GGG - chr5.1:21576794-21576813 MS.gene068613:CDS 35.0%
AACTTACCTCTTGATGTTGC+CGG - chr5.1:21577295-21577314 MS.gene068613:CDS 40.0%
ACCACATGTGACTGTTTGTT+GGG + chr5.1:21577188-21577207 None:intergenic 40.0%
CACATTAGCACCAAAAGCTT+TGG + chr5.1:21577443-21577462 None:intergenic 40.0%
CCATCTATCCATTAGTTCCA+GGG - chr5.1:21576863-21576882 MS.gene068613:CDS 40.0%
GTAGCTGGAAGTTTAATTGG+AGG - chr5.1:21577933-21577952 MS.gene068613:intron 40.0%
TAAATGCAGGCATGAACTTG+CGG - chr5.1:21577036-21577055 MS.gene068613:CDS 40.0%
TCAAATACCTGCATCTGATC+TGG + chr5.1:21577547-21577566 None:intergenic 40.0%
TCCATCTATCCATTAGTTCC+AGG - chr5.1:21576862-21576881 MS.gene068613:CDS 40.0%
TGATTATGTCAACACAGCAG+TGG - chr5.1:21578031-21578050 MS.gene068613:CDS 40.0%
TGGAGGTTATTCTGACTCAA+TGG - chr5.1:21577237-21577256 MS.gene068613:CDS 40.0%
! AGTGTTAGAGCATCCTAAGA+AGG - chr5.1:21576448-21576467 MS.gene068613:CDS 40.0%
! CCTTCTGGAGAAATTGAAAG+GGG + chr5.1:21576513-21576532 None:intergenic 40.0%
! GAAAATACAGGAAGCTCTAG+AGG + chr5.1:21577774-21577793 None:intergenic 40.0%
! GTGAAATTCGCCAAAGCTTT+TGG - chr5.1:21577430-21577449 MS.gene068613:CDS 40.0%
!! GCATTCAAGGTGTTGTATTG+TGG - chr5.1:21576793-21576812 MS.gene068613:CDS 40.0%
AAATGCAGGCATGAACTTGC+GGG - chr5.1:21577037-21577056 MS.gene068613:CDS 45.0%
AGTTGGAGACAAAGTAGGTG+TGG - chr5.1:21577099-21577118 MS.gene068613:CDS 45.0%
ATCCTAAGAAGGCCTTTGGT+TGG - chr5.1:21576459-21576478 MS.gene068613:CDS 45.0%
CAAAGTAGGTGTGGGATACA+TGG - chr5.1:21577108-21577127 MS.gene068613:CDS 45.0%
CACCACATGTGACTGTTTGT+TGG + chr5.1:21577189-21577208 None:intergenic 45.0%
CCCCTTTCAATTTCTCCAGA+AGG - chr5.1:21576510-21576529 MS.gene068613:CDS 45.0%
GAAGCATACCCTGGAACTAA+TGG + chr5.1:21576874-21576893 None:intergenic 45.0%
GCTGCAACAGGTACTTTGAA+TGG - chr5.1:21577651-21577670 MS.gene068613:intron 45.0%
GGAAAGCTTGTAATGGTTGG+TGG - chr5.1:21577738-21577757 MS.gene068613:CDS 45.0%
GTAGGGAGAAAGTTAGTAGC+TGG - chr5.1:21577918-21577937 MS.gene068613:intron 45.0%
GTTGGAGACAAAGTAGGTGT+GGG - chr5.1:21577100-21577119 MS.gene068613:CDS 45.0%
TCCTAAGAAGGCCTTTGGTT+GGG - chr5.1:21576460-21576479 MS.gene068613:CDS 45.0%
TCGAGTCCAAAATGTCGAAG+AGG + chr5.1:21577355-21577374 None:intergenic 45.0%
TTCCACTTTGCTTCCTACCT+CGG + chr5.1:21577071-21577090 None:intergenic 45.0%
! CACATGTGACTGTTTGTTGG+GGG + chr5.1:21577186-21577205 None:intergenic 45.0%
! CATTTTGGACTCGACAAACC+TGG - chr5.1:21577361-21577380 MS.gene068613:CDS 45.0%
! CCACATGTGACTGTTTGTTG+GGG + chr5.1:21577187-21577206 None:intergenic 45.0%
! CCCTGGAACTAATGGATAGA+TGG + chr5.1:21576866-21576885 None:intergenic 45.0%
!! ATAGGTGTTGTTGGTCTTGG+TGG - chr5.1:21577391-21577410 MS.gene068613:CDS 45.0%
!! TCTTGGTGGACTTGGTCATA+TGG - chr5.1:21577405-21577424 MS.gene068613:CDS 45.0%
AAAGGGGAGAGAACACCAGA+AGG + chr5.1:21576497-21576516 None:intergenic 50.0%
AACCGAGGTAGGAAGCAAAG+TGG - chr5.1:21577066-21577085 MS.gene068613:CDS 50.0%
ACTTGCGGGTATAGTAACCG+AGG - chr5.1:21577051-21577070 MS.gene068613:CDS 50.0%
ATCTCTAGCTGCCCAACCAA+AGG + chr5.1:21576474-21576493 None:intergenic 50.0%
CCCCAACAAACAGTCACATG+TGG - chr5.1:21577184-21577203 MS.gene068613:CDS 50.0%
GAGCATCCTAAGAAGGCCTT+TGG - chr5.1:21576455-21576474 MS.gene068613:CDS 50.0%
! AAGGAGTTGGAAGCATACCC+TGG + chr5.1:21576883-21576902 None:intergenic 50.0%
! TGTGGTGCCAAGTATCGTGA+TGG - chr5.1:21577202-21577221 MS.gene068613:CDS 50.0%
!! GCTTGTAATGGTTGGTGGAG+TGG - chr5.1:21577743-21577762 MS.gene068613:CDS 50.0%
!! GTTGTTGGTCTTGGTGGACT+TGG - chr5.1:21577397-21577416 MS.gene068613:CDS 50.0%
AGAGGACCGGCAACATCAAG+AGG + chr5.1:21577304-21577323 None:intergenic 55.0%
CGTGATGGCACCGTCACATA+TGG - chr5.1:21577217-21577236 MS.gene068613:CDS 55.0%
CTGCGATCCAGATCAGATGC+AGG - chr5.1:21577537-21577556 MS.gene068613:intron 55.0%
GATGGCACCGTCACATATGG+AGG - chr5.1:21577220-21577239 MS.gene068613:CDS 55.0%
GCCCAACCAAAGGCCTTCTT+AGG + chr5.1:21576464-21576483 None:intergenic 55.0%
GCGGGTATAGTAACCGAGGT+AGG - chr5.1:21577055-21577074 MS.gene068613:CDS 55.0%
GTGACTCCAGCGCAAAGAAG+AGG + chr5.1:21577322-21577341 None:intergenic 55.0%
TCCAGCGCAAAGAAGAGGAC+CGG + chr5.1:21577317-21577336 None:intergenic 55.0%
TGGGCAGCTAGAGATCCTTC+TGG - chr5.1:21576479-21576498 MS.gene068613:CDS 55.0%
!! GACGGTGCCATCACGATACT+TGG + chr5.1:21577212-21577231 None:intergenic 55.0%
GCCGGTCCTCTTCTTTGCGC+TGG - chr5.1:21577313-21577332 MS.gene068613:CDS 65.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 21576431 21578127 21576431 ID=MS.gene068613
chr5.1 mRNA 21576431 21578127 21576431 ID=MS.gene068613.t1;Parent=MS.gene068613
chr5.1 exon 21578027 21578127 21578027 ID=MS.gene068613.t1.exon1;Parent=MS.gene068613.t1
chr5.1 CDS 21578027 21578127 21578027 ID=cds.MS.gene068613.t1;Parent=MS.gene068613.t1
chr5.1 exon 21577674 21577787 21577674 ID=MS.gene068613.t1.exon2;Parent=MS.gene068613.t1
chr5.1 CDS 21577674 21577787 21577674 ID=cds.MS.gene068613.t1;Parent=MS.gene068613.t1
chr5.1 exon 21577000 21577513 21577000 ID=MS.gene068613.t1.exon3;Parent=MS.gene068613.t1
chr5.1 CDS 21577000 21577513 21577000 ID=cds.MS.gene068613.t1;Parent=MS.gene068613.t1
chr5.1 exon 21576754 21576907 21576754 ID=MS.gene068613.t1.exon4;Parent=MS.gene068613.t1
chr5.1 CDS 21576754 21576907 21576754 ID=cds.MS.gene068613.t1;Parent=MS.gene068613.t1
chr5.1 exon 21576431 21576636 21576431 ID=MS.gene068613.t1.exon5;Parent=MS.gene068613.t1
chr5.1 CDS 21576431 21576636 21576431 ID=cds.MS.gene068613.t1;Parent=MS.gene068613.t1
Gene Sequence

>MS.gene068613

ATGGCAACAGAAACTAAAGTGTTAGAGCATCCTAAGAAGGCCTTTGGTTGGGCAGCTAGAGATCCTTCTGGTGTTCTCTCCCCTTTCAATTTCTCCAGAAGGTTTACTTCATTATAATCAACTCATCTTTATTTAATGGATTGAAATAATATTTGATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATGTATGTATGTATGTATGTATGTATGTATGTATGTATGTATGTATGTATATTTTTTTTTACTTAATTTAGATCTATGTTGTTGCCTACTAATTAAGTCAACTTTGGTGCATCAAAACTTTAGGGAAACTGGTGAGAAAGATGTAGCATTCAAGGTGTTGTATTGTGGGATATGTCACACTGACCTTCATATGATGAAGAATGAATGGGGCAATTCCATCTATCCATTAGTTCCAGGGTATGCTTCCAACTCCTTCTACTACAATTATACTTTATTCCTACGTTACTTGGACCATATATATCTTTATACTTACTTTAATGATGAGAGAGTGAAAAGTTGAATGAGTCAATAAAAAGTAAGAGTATATTTGAAATTTGCTTATAATTAATAAATGCAGGCATGAACTTGCGGGTATAGTAACCGAGGTAGGAAGCAAAGTGGAAAAGTTTAAAGTTGGAGACAAAGTAGGTGTGGGATACATGGTTGATTCATGTCGTTCATGCGAAAATTGTGCTGACGATCTTGAGAATTACTGCCCCCAACAAACAGTCACATGTGGTGCCAAGTATCGTGATGGCACCGTCACATATGGAGGTTATTCTGACTCAATGGTTGCAGACGAGCACTTTGTGATTCGCATTCCTGATAACTTACCTCTTGATGTTGCCGGTCCTCTTCTTTGCGCTGGAGTCACAGTGTATAGTCCTCTTCGACATTTTGGACTCGACAAACCTGGTATGAATATAGGTGTTGTTGGTCTTGGTGGACTTGGTCATATGGCTGTGAAATTCGCCAAAGCTTTTGGTGCTAATGTGACCGTAATTAGCACATCTCGTAACAAAGAAAAGGAAGCAATAGAACACTTGGGAGCTGACTCGTTTCTTGTAAGCTGCGATCCAGATCAGATGCAGGTATTTGATTCATTTGATTAATTTGTGAATTTCATTATTTCAGAAATCGAGAATGTTAATCAAGAAACTAATAATGTACCACATTTTAATAGGCTGCAACAGGTACTTTGAATGGTATTATTGACACGGTTTCAGCGAGTCATCCTATTTTACCATTGATTGGTTTATTGAAATCTAATGGAAAGCTTGTAATGGTTGGTGGAGTGGCAAAGCCTCTAGAGCTTCCTGTATTTTCTTTACTTGGAGGTAAGCATATAAAAAATATTTCGATAGTATAGTTTTAAGACTAAATGCTAAATATGCAGTTTTAAATTCAAATTATGCAGAATGTTGATACTTGTATAAATTTCACATTATTTGTAGGGAGAAAGTTAGTAGCTGGAAGTTTAATTGGAGGGATAAAGGAGACTCAAGAAATGATTGATTTTGCGGCAAAACACAATGTAAAGCCTGATATTGAGGTTATTCCTATTGATTATGTCAACACAGCAGTGGAGCGTCTCGAGAAAGCAGACGTGAAATATCGATTCGTGATTGATATTGGAAATACGCTGAAACCAAGCCCTTAA
Protein sequence

>MS.gene068613.t1

MATETKVLEHPKKAFGWAARDPSGVLSPFNFSRRETGEKDVAFKVLYCGICHTDLHMMKNEWGNSIYPLVPGHELAGIVTEVGSKVEKFKVGDKVGVGYMVDSCRSCENCADDLENYCPQQTVTCGAKYRDGTVTYGGYSDSMVADEHFVIRIPDNLPLDVAGPLLCAGVTVYSPLRHFGLDKPGMNIGVVGLGGLGHMAVKFAKAFGANVTVISTSRNKEKEAIEHLGADSFLVSCDPDQMQAATGTLNGIIDTVSASHPILPLIGLLKSNGKLVMVGGVAKPLELPVFSLLGGRKLVAGSLIGGIKETQEMIDFAAKHNVKPDIEVIPIDYVNTAVERLEKADVKYRFVIDIGNTLKPSP