AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene027825

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027825.t1 MTR_5g031190 97.784 361 8 0 1 361 1 361 0.0 726
MS.gene027825.t1 MTR_5g031180 86.704 361 48 0 1 361 1 361 0.0 608
MS.gene027825.t1 MTR_8g068190 81.389 360 67 0 1 360 1 360 0.0 579
MS.gene027825.t1 MTR_5g031250 77.562 361 81 0 1 361 1 361 0.0 574
MS.gene027825.t1 MTR_5g031390 76.731 361 84 0 1 361 1 361 0.0 572
MS.gene027825.t1 MTR_5g031230 76.177 361 86 0 1 361 1 361 0.0 566
MS.gene027825.t1 MTR_5g031310 77.337 353 80 0 8 360 9 361 0.0 563
MS.gene027825.t1 MTR_5g031360 76.923 351 81 0 10 360 12 362 0.0 560
MS.gene027825.t1 MTR_4g115300 75.069 361 90 0 1 361 1 361 0.0 558
MS.gene027825.t1 MTR_5g031305 76.771 353 82 0 8 360 9 361 0.0 557
MS.gene027825.t1 MTR_5g031300 73.729 354 93 0 8 361 9 362 0.0 545
MS.gene027825.t1 MTR_5g031210 74.515 361 92 0 1 361 1 361 0.0 538
MS.gene027825.t1 MTR_8g092630 64.146 357 127 1 3 359 60 415 5.33e-153 437
MS.gene027825.t1 MTR_5g031430 59.777 358 144 0 1 358 1 358 1.93e-147 421
MS.gene027825.t1 MTR_1g025950 57.627 354 149 1 8 360 7 360 9.57e-138 396
MS.gene027825.t1 MTR_5g031250 76.349 241 57 0 1 241 1 241 5.10e-133 380
MS.gene027825.t1 MTR_1g107425 52.770 343 162 0 11 353 9 351 1.82e-124 362
MS.gene027825.t1 MTR_2g009810 54.046 346 157 2 14 358 62 406 6.42e-123 360
MS.gene027825.t1 MTR_1g107425 52.083 288 138 0 11 298 9 296 4.54e-99 296
MS.gene027825.t1 MTR_2g009820 50.400 250 114 4 116 358 8 254 6.46e-69 217
MS.gene027825.t1 MTR_5g031290 73.684 114 30 0 247 360 80 193 1.44e-54 178
MS.gene027825.t1 MTR_5g031290 80.000 80 15 1 1 79 1 80 1.34e-40 142
MS.gene027825.t1 MTR_8g095220 26.786 280 175 7 25 278 68 343 1.24e-23 101
MS.gene027825.t1 MTR_8g095220 28.099 242 149 6 25 245 68 305 3.79e-23 98.6
MS.gene027825.t1 MTR_3g089977 24.074 378 243 15 11 354 12 379 8.68e-19 87.0
MS.gene027825.t1 MTR_3g089940 24.457 368 235 14 11 344 12 370 1.21e-18 86.7
MS.gene027825.t1 MTR_3g089940 24.412 340 217 12 39 344 37 370 2.25e-18 85.9
MS.gene027825.t1 MTR_0001s0660 26.159 302 199 9 38 318 35 333 4.62e-18 85.5
MS.gene027825.t1 MTR_3g089940 24.702 336 218 12 11 319 12 339 1.24e-16 80.5
MS.gene027825.t1 MTR_6g466320 26.000 300 198 9 1 288 6 293 1.84e-16 80.1
MS.gene027825.t1 MTR_6g465010 26.000 300 198 9 1 288 6 293 1.84e-16 80.1
MS.gene027825.t1 MTR_3g089940 24.405 336 219 12 11 319 12 339 2.08e-16 79.7
MS.gene027825.t1 MTR_3g089970 23.824 340 219 12 39 344 38 371 2.28e-16 80.1
MS.gene027825.t1 MTR_6g465010 26.937 271 177 7 30 288 16 277 2.73e-16 79.3
MS.gene027825.t1 MTR_1g069430 23.810 336 227 8 1 312 1 331 3.60e-14 73.6
MS.gene027825.t1 MTR_7g068320 26.648 349 219 14 39 353 48 393 4.50e-14 73.2
MS.gene027825.t1 MTR_5g045710 24.918 305 200 9 38 318 35 334 7.69e-14 72.4
MS.gene027825.t1 MTR_7g099040 23.899 318 207 9 38 327 37 347 3.54e-13 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027825.t1 AT4G37970 66.197 355 120 0 6 360 7 361 8.09e-174 488
MS.gene027825.t1 AT4G37980 72.000 350 98 0 11 360 7 356 1.91e-173 486
MS.gene027825.t1 AT4G37990 72.571 350 96 0 11 360 7 356 3.09e-172 484
MS.gene027825.t1 AT4G39330 63.585 357 130 0 1 357 1 357 1.65e-169 477
MS.gene027825.t1 AT2G21730 61.891 349 131 2 11 357 5 353 7.88e-157 445
MS.gene027825.t1 AT2G21890 60.920 348 135 1 11 357 5 352 2.57e-155 441
MS.gene027825.t1 AT4G39330 62.783 309 115 0 1 309 1 309 1.23e-139 399
MS.gene027825.t1 AT4G37980 72.887 284 77 0 11 294 7 290 9.75e-136 389
MS.gene027825.t1 AT4G37970 65.398 289 100 0 6 294 7 295 1.76e-134 385
MS.gene027825.t1 AT3G19450 54.335 346 158 0 11 356 9 354 9.44e-119 348
MS.gene027825.t1 AT1G72680 48.324 358 182 2 1 358 1 355 1.40e-113 335
MS.gene027825.t1 AT4G34230 52.975 353 165 1 8 359 5 357 5.76e-109 323
MS.gene027825.t1 AT4G34230 52.691 353 166 1 8 359 5 357 3.95e-108 321
MS.gene027825.t1 AT4G37970 64.979 237 83 0 6 242 7 243 9.40e-107 313
MS.gene027825.t1 AT4G37970 64.979 237 83 0 6 242 7 243 9.71e-107 313
MS.gene027825.t1 AT5G63620 27.491 291 181 7 15 278 58 345 4.33e-25 105
MS.gene027825.t1 AT5G63620 27.491 291 181 7 15 278 58 345 5.16e-25 105
MS.gene027825.t1 AT5G63620 27.888 251 158 5 15 244 58 306 7.26e-24 100
MS.gene027825.t1 AT5G51970 27.174 276 178 8 15 278 21 285 8.35e-18 84.0
MS.gene027825.t1 AT5G51970 27.174 276 178 8 15 278 21 285 8.35e-18 84.0
MS.gene027825.t1 AT1G64710 24.516 310 199 10 39 318 13 317 1.34e-16 80.5
MS.gene027825.t1 AT1G64710 24.516 310 199 10 39 318 36 340 1.59e-16 80.5
MS.gene027825.t1 AT5G43940 24.262 305 205 6 39 319 36 338 2.02e-16 80.1
MS.gene027825.t1 AT5G43940 24.262 305 205 6 39 319 48 350 2.13e-16 80.1
MS.gene027825.t1 AT1G77120 23.631 347 230 13 39 354 36 378 1.57e-15 77.4
MS.gene027825.t1 AT1G32780 25.275 364 221 15 39 358 37 393 1.45e-13 71.6
MS.gene027825.t1 AT1G22430 26.633 199 127 5 39 219 42 239 5.07e-12 66.2
MS.gene027825.t1 AT1G22430 26.633 199 127 5 39 219 42 239 5.07e-12 66.2

Find 97 sgRNAs with CRISPR-Local

Find 159 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATAGCCCAATGAAACATTT+TGG 0.194725 5.2:-21528795 MS.gene027825:CDS
GTGGTTGGTCTTGGTGGATT+AGG 0.267262 5.2:-21528744 MS.gene027825:CDS
CTTGGTCTATTGAAGAATAA+TGG 0.272969 5.2:-21528392 MS.gene027825:CDS
GTGAAGTTCGCCAAAGCTTT+TGG 0.308390 5.2:-21528711 MS.gene027825:CDS
TCTAATCCAAAATGTTTCAT+TGG 0.321961 5.2:+21528789 None:intergenic
GCTGCTCCTCTCCTTTGTGC+TGG 0.345949 5.2:-21528828 MS.gene027825:CDS
CTCCTTTCAAGTTCTCTAGA+AGG 0.347990 5.2:-21529967 MS.gene027825:intron
GATAGTGCCATCAACATTCT+TGG 0.354624 5.2:+21528932 None:intergenic
GGTTGGTGTGGGGTGCATGG+TGG 0.358726 5.2:-21529030 MS.gene027825:CDS
GTTGGTGTGGGGTGCATGGT+GGG 0.372899 5.2:-21529029 MS.gene027825:CDS
GCTGTAATGGGCACGTTTGA+TGG 0.372955 5.2:-21528458 MS.gene027825:CDS
GGAAACTCCAAAGGCTTCTC+TGG 0.377400 5.2:+21528347 None:intergenic
CAGGGTTGGTGTGGGGTGCA+TGG 0.379254 5.2:-21529033 MS.gene027825:CDS
CTAATCCAAAATGTTTCATT+GGG 0.384957 5.2:+21528790 None:intergenic
CCCACAATCAAAGAAAATAC+TGG 0.392755 5.2:+21528326 None:intergenic
GAATAATGGTAAACTTGTTA+TGG 0.398732 5.2:-21528378 MS.gene027825:CDS
TACCTTTACACCAAAAGCTT+TGG 0.401200 5.2:+21528701 None:intergenic
CAATATGTTATAGGCTGTAA+TGG 0.405959 5.2:-21528471 MS.gene027825:intron
ACTGGAACAACCTCAATATC+AGG 0.408861 5.2:+21528132 None:intergenic
AGACCAAGCAAAGGCATTAG+AGG 0.414126 5.2:+21528407 None:intergenic
ACTACTTACCCAGTAGTTCC+TGG 0.414223 5.2:-21529233 MS.gene027825:CDS
ACAATTGGTGGGAGTATGAT+TGG 0.420703 5.2:-21528210 MS.gene027825:CDS
TATTGGTACGCACCTTCATC+TGG 0.421150 5.2:+21528592 None:intergenic
TCTTGGTGGATTAGGTCATA+TGG 0.422443 5.2:-21528736 MS.gene027825:CDS
CATATAGGTGTGGTTGGTCT+TGG 0.424100 5.2:-21528753 MS.gene027825:CDS
CTGCTCCTCTCCTTTGTGCT+GGG 0.424848 5.2:-21528827 MS.gene027825:CDS
TACAGTTCCAAGAATGTTGA+TGG 0.430392 5.2:-21528939 MS.gene027825:CDS
ATAGGTGTGGTTGGTCTTGG+TGG 0.433205 5.2:-21528750 MS.gene027825:CDS
TATCGATTTGTTATTGACAT+TGG 0.438397 5.2:-21528060 MS.gene027825:CDS
CATCCTCTAATGCCTTTGCT+TGG 0.446345 5.2:-21528410 MS.gene027825:CDS
ATGCTCCGTTTCTGGATTTG+AGG 0.446888 5.2:+21530039 None:intergenic
CGCGACAACTTTGACATGAA+TGG 0.451193 5.2:+21529000 None:intergenic
TGGAGCTCCAGAGAAGCCTT+TGG 0.454095 5.2:-21528354 MS.gene027825:CDS
TTAGAGTACCCAGGAACTAC+TGG 0.455836 5.2:+21529224 None:intergenic
AATATGTTATAGGCTGTAAT+GGG 0.459443 5.2:-21528470 MS.gene027825:intron
AAAACTTCAAAGTTGGAGAC+AGG 0.459613 5.2:-21529052 MS.gene027825:CDS
TGTTGAAGACATGAAGTTGC+TGG 0.463326 5.2:-21529104 MS.gene027825:intron
GTTGATGGCACTATCACATA+TGG 0.465804 5.2:-21528924 MS.gene027825:CDS
AGCAGCAGCATCAAGTGATA+AGG 0.468455 5.2:+21528845 None:intergenic
AATGGTAAACTTGTTATGGT+TGG 0.469139 5.2:-21528374 MS.gene027825:CDS
AGTTGGAGACAGGGTTGGTG+TGG 0.488731 5.2:-21529042 MS.gene027825:CDS
AAGCTTTCGGATGGGCAGCT+AGG 0.490753 5.2:-21530009 MS.gene027825:CDS
GCATCAAGTGATAAGGCATC+AGG 0.495160 5.2:+21528852 None:intergenic
GTGATAAGGCATCAGGAATA+CGG 0.500674 5.2:+21528859 None:intergenic
AGCTTTCGGATGGGCAGCTA+GGG 0.501441 5.2:-21530008 MS.gene027825:CDS
GACAAACCTGGACTGCATAT+AGG 0.502372 5.2:-21528768 MS.gene027825:CDS
TGGGCAGCTAGGGACACATC+TGG 0.503109 5.2:-21529998 MS.gene027825:CDS
GAGTATGATTGGAGGGATGA+AGG 0.509991 5.2:-21528199 MS.gene027825:CDS
TTTCAGGGAAACAGGGGAGA+AGG 0.511637 5.2:-21529330 MS.gene027825:intron
GGAAACAGGGGAGAAGGATG+TGG 0.515934 5.2:-21529324 MS.gene027825:CDS
GTTGGAGACAGGGTTGGTGT+GGG 0.523836 5.2:-21529041 MS.gene027825:CDS
TAGAGAACTTGAAAGGAGAA+AGG 0.526536 5.2:+21529972 None:intergenic
GTCAGCTCCTAAGTGTTCAA+TGG 0.541539 5.2:+21528641 None:intergenic
TTGGCAGGGAGAAAGACAAT+TGG 0.543249 5.2:-21528225 MS.gene027825:intron
GCAGGGAGAAAGACAATTGG+TGG 0.544871 5.2:-21528222 MS.gene027825:intron
TGACATTGGAAACACAATGA+AGG 0.546508 5.2:-21528046 MS.gene027825:CDS
TAGAGGATGGAGTGCTGAAA+CGG 0.546944 5.2:+21528424 None:intergenic
TGATATTGAGGTTGTTCCAG+TGG 0.550536 5.2:-21528130 MS.gene027825:CDS
GCAGTATTTACATAATCCAC+TGG 0.550557 5.2:+21528114 None:intergenic
ACCACACCTATATGCAGTCC+AGG 0.552769 5.2:+21528762 None:intergenic
CTTAGGAGCTGACTCATTCG+TGG 0.554856 5.2:-21528631 MS.gene027825:CDS
AAGGAAGCCATTGAACACTT+AGG 0.565621 5.2:-21528648 MS.gene027825:CDS
AATGGCCTCAAATCCAGAAA+CGG 0.577912 5.2:-21530044 MS.gene027825:CDS
CAAGCAAAGGCATTAGAGGA+TGG 0.578766 5.2:+21528411 None:intergenic
TTCAAAGTTGGAGACAGGGT+TGG 0.579645 5.2:-21529047 MS.gene027825:CDS
GAAAATACTGGAAACTCCAA+AGG 0.580584 5.2:+21528338 None:intergenic
TGGCGTAAAACCTGATATTG+AGG 0.586456 5.2:-21528142 MS.gene027825:CDS
CTACTTACCCAGTAGTTCCT+GGG 0.589206 5.2:-21529232 MS.gene027825:intron
TTGGTGGGAGTATGATTGGA+GGG 0.589557 5.2:-21528206 MS.gene027825:CDS
ACAACTTTGACATGAATGGC+AGG 0.589783 5.2:+21529004 None:intergenic
ATTGGTGGGAGTATGATTGG+AGG 0.590145 5.2:-21528207 MS.gene027825:CDS
AGTGACAGAGGTGGGAAGCA+AGG 0.592868 5.2:-21529078 MS.gene027825:CDS
GGACTGCATATAGGTGTGGT+TGG 0.595201 5.2:-21528759 MS.gene027825:CDS
TACCTTCTAGAGAACTTGAA+AGG 0.595565 5.2:+21529965 None:intergenic
CCACATGGCTAAGAATGAGT+GGG 0.595627 5.2:-21529261 MS.gene027825:CDS
GGATTATGTAAATACTGCAA+TGG 0.611722 5.2:-21528109 MS.gene027825:CDS
TCCACATGGCTAAGAATGAG+TGG 0.611827 5.2:-21529262 MS.gene027825:CDS
AAACTTCAAAGTTGGAGACA+GGG 0.615703 5.2:-21529051 MS.gene027825:CDS
TTGGAGACAGGGTTGGTGTG+GGG 0.621846 5.2:-21529040 MS.gene027825:CDS
TAGAGTACCCAGGAACTACT+GGG 0.622352 5.2:+21529225 None:intergenic
TTCTTCAATAGACCAAGCAA+AGG 0.622643 5.2:+21528398 None:intergenic
ACTCATTCTTAGCCATGTGG+AGG 0.625005 5.2:+21529264 None:intergenic
ATGTCACTCGGACCTCCACA+TGG 0.635155 5.2:-21529276 MS.gene027825:CDS
TCGCGAACAAGACCAGATGA+AGG 0.635463 5.2:-21528604 MS.gene027825:intron
GCTGGTGAAGTGACAGAGGT+GGG 0.643936 5.2:-21529086 MS.gene027825:CDS
CCCACTCATTCTTAGCCATG+TGG 0.645286 5.2:+21529261 None:intergenic
CAGGGAGAAAGACAATTGGT+GGG 0.647955 5.2:-21528221 MS.gene027825:CDS
GTGATCCCAGCACAAAGGAG+AGG 0.651936 5.2:+21528822 None:intergenic
GATGGCACTATCACATATGG+AGG 0.655594 5.2:-21528921 MS.gene027825:CDS
TGCTGGTGAAGTGACAGAGG+TGG 0.668343 5.2:-21529087 MS.gene027825:CDS
CACATGGCTAAGAATGAGTG+GGG 0.673842 5.2:-21529260 MS.gene027825:CDS
TGGAGGCTACTCTGACACCA+TGG 0.680550 5.2:-21528904 MS.gene027825:CDS
TTCATGTCAAAGTTGTCGCG+AGG 0.686790 5.2:-21528997 MS.gene027825:CDS
ACCTGGACTGCATATAGGTG+TGG 0.690593 5.2:-21528763 MS.gene027825:CDS
AAAGTGTTCGTCGACAACCA+TGG 0.696527 5.2:+21528887 None:intergenic
ACACTGTGATCCCAGCACAA+AGG 0.700831 5.2:+21528817 None:intergenic
AGTTGCTGGTGAAGTGACAG+AGG 0.727660 5.2:-21529090 MS.gene027825:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTGAAAATTAAATAAACAA+TGG + chr5.2:21528894-21528913 None:intergenic 10.0%
!! ATCCATAAAAAAAAAAAATC+AGG + chr5.2:21528576-21528595 None:intergenic 15.0%
!! TCATTATAATAATTTCACTA+AGG + chr5.2:21528164-21528183 None:intergenic 15.0%
!! TTACTATATAGAAATAGATT+TGG + chr5.2:21529539-21529558 None:intergenic 15.0%
!!! AAAATTAGTTTGTTTTATGA+TGG + chr5.2:21528932-21528951 None:intergenic 15.0%
!!! ATCCTGATTTTTTTTTTTTA+TGG - chr5.2:21528571-21528590 MS.gene027825:intron 15.0%
!!! GACATATGATTTATTTTAAT+TGG - chr5.2:21528644-21528663 MS.gene027825:CDS 15.0%
!! AAGTTAAAAGATTTAAAGAC+AGG - chr5.2:21528469-21528488 MS.gene027825:intron 20.0%
!! AATGTTGTTCAATATGTTAT+AGG - chr5.2:21529598-21529617 MS.gene027825:intron 20.0%
!! AGTTAAAAGATTTAAAGACA+GGG - chr5.2:21528470-21528489 MS.gene027825:intron 20.0%
!! ATATACATCACATTAATTGT+TGG - chr5.2:21529834-21529853 MS.gene027825:intron 20.0%
!! TTGAACAACATTAGAATTAA+AGG + chr5.2:21529590-21529609 None:intergenic 20.0%
!!! AATATTTTTCATTCCTTTTC+AGG - chr5.2:21528732-21528751 MS.gene027825:CDS 20.0%
!!! ATATTTTTCATTCCTTTTCA+GGG - chr5.2:21528733-21528752 MS.gene027825:CDS 20.0%
! AATATGTTATAGGCTGTAAT+GGG - chr5.2:21529608-21529627 MS.gene027825:intron 25.0%
! AATGTTTGATGAAGGAAAAA+AGG + chr5.2:21528226-21528245 None:intergenic 25.0%
! AGTCATTAAAAGCGAATTAT+TGG + chr5.2:21529506-21529525 None:intergenic 25.0%
! ATGTTTGATGAAGGAAAAAA+GGG + chr5.2:21528225-21528244 None:intergenic 25.0%
! CTAATCCAAAATGTTTCATT+GGG + chr5.2:21529291-21529310 None:intergenic 25.0%
! TCTAATCCAAAATGTTTCAT+TGG + chr5.2:21529292-21529311 None:intergenic 25.0%
!!! GAATAATGGTAAACTTGTTA+TGG - chr5.2:21529700-21529719 MS.gene027825:intron 25.0%
!!! TATCGATTTGTTATTGACAT+TGG - chr5.2:21530018-21530037 MS.gene027825:CDS 25.0%
!!! TTTTTTCCTTCATCAAACAT+TGG - chr5.2:21528225-21528244 MS.gene027825:intron 25.0%
AAGAAACATCTCATAAACGA+TGG - chr5.2:21528393-21528412 MS.gene027825:CDS 30.0%
AAGGTGAAAAACTTCAAAGT+TGG - chr5.2:21529019-21529038 MS.gene027825:CDS 30.0%
ACTAAAAATATAAACCGACG+TGG + chr5.2:21528269-21528288 None:intergenic 30.0%
AGAAACATCTCATAAACGAT+GGG - chr5.2:21528394-21528413 MS.gene027825:CDS 30.0%
CAATATGTTATAGGCTGTAA+TGG - chr5.2:21529607-21529626 MS.gene027825:intron 30.0%
CTAAAAATATAAACCGACGT+GGG + chr5.2:21528268-21528287 None:intergenic 30.0%
CTTGGTCTATTGAAGAATAA+TGG - chr5.2:21529686-21529705 MS.gene027825:intron 30.0%
GGATTATGTAAATACTGCAA+TGG - chr5.2:21529969-21529988 MS.gene027825:CDS 30.0%
TACTTCTCCTAACAAAAAGA+AGG - chr5.2:21529411-21529430 MS.gene027825:intron 30.0%
TGCTTAAACAATGCTAATCA+AGG - chr5.2:21528315-21528334 MS.gene027825:intron 30.0%
TTAAACAATGCTAATCAAGG+TGG - chr5.2:21528318-21528337 MS.gene027825:intron 30.0%
! ATCTCTTATGTTTTCTCAAG+CGG + chr5.2:21528507-21528526 None:intergenic 30.0%
! CCAGTATTTTCTTTGATTGT+GGG - chr5.2:21529752-21529771 MS.gene027825:intron 30.0%
! TATAGCCCAATGAAACATTT+TGG - chr5.2:21529283-21529302 MS.gene027825:CDS 30.0%
! TCCAGTATTTTCTTTGATTG+TGG - chr5.2:21529751-21529770 MS.gene027825:intron 30.0%
!! AATGGTAAACTTGTTATGGT+TGG - chr5.2:21529704-21529723 MS.gene027825:intron 30.0%
!! GCATTCAAAGTACTATTTTG+CGG - chr5.2:21528776-21528795 MS.gene027825:CDS 30.0%
AAAACTTCAAAGTTGGAGAC+AGG - chr5.2:21529026-21529045 MS.gene027825:CDS 35.0%
AAACTTCAAAGTTGGAGACA+GGG - chr5.2:21529027-21529046 MS.gene027825:CDS 35.0%
ACATCACATTAATTGTTGGC+AGG - chr5.2:21529838-21529857 MS.gene027825:intron 35.0%
CATCACATTAATTGTTGGCA+GGG - chr5.2:21529839-21529858 MS.gene027825:intron 35.0%
CCCACAATCAAAGAAAATAC+TGG + chr5.2:21529755-21529774 None:intergenic 35.0%
GAAAATACTGGAAACTCCAA+AGG + chr5.2:21529743-21529762 None:intergenic 35.0%
GAAACATCTCATAAACGATG+GGG - chr5.2:21528395-21528414 MS.gene027825:CDS 35.0%
GCAGTATTTACATAATCCAC+TGG + chr5.2:21529967-21529986 None:intergenic 35.0%
TACAGTTCCAAGAATGTTGA+TGG - chr5.2:21529139-21529158 MS.gene027825:intron 35.0%
TACATCACACATCAACATTC+AGG + chr5.2:21528549-21528568 None:intergenic 35.0%
TACCTTTACACCAAAAGCTT+TGG + chr5.2:21529380-21529399 None:intergenic 35.0%
TAGAGAACTTGAAAGGAGAA+AGG + chr5.2:21528109-21528128 None:intergenic 35.0%
TGACATTGGAAACACAATGA+AGG - chr5.2:21530032-21530051 MS.gene027825:CDS 35.0%
TTCTTCAATAGACCAAGCAA+AGG + chr5.2:21529683-21529702 None:intergenic 35.0%
! ATGGCTTCCTTCTTTTTGTT+AGG + chr5.2:21529421-21529440 None:intergenic 35.0%
! ATTTTGCGGTATATGTCACT+CGG - chr5.2:21528790-21528809 MS.gene027825:CDS 35.0%
! TAATCAAGGTGGTTTTCACT+TGG - chr5.2:21528329-21528348 MS.gene027825:CDS 35.0%
! TACCTTCTAGAGAACTTGAA+AGG + chr5.2:21528116-21528135 None:intergenic 35.0%
! TCTCTTATGTTTTCTCAAGC+GGG + chr5.2:21528506-21528525 None:intergenic 35.0%
!! CATTTTGGATTAGACAAACC+TGG - chr5.2:21529298-21529317 MS.gene027825:CDS 35.0%
!! TTTTGGTGTAAAGGTAACTG+TGG - chr5.2:21529384-21529403 MS.gene027825:intron 35.0%
!! TTTTTGATGTTAGAGTACCC+AGG + chr5.2:21528866-21528885 None:intergenic 35.0%
AAGGAAGCCATTGAACACTT+AGG - chr5.2:21529430-21529449 MS.gene027825:intron 40.0%
ACAACTTTGACATGAATGGC+AGG + chr5.2:21529077-21529096 None:intergenic 40.0%
ACTGGAACAACCTCAATATC+AGG + chr5.2:21529949-21529968 None:intergenic 40.0%
AGGCTGCATGAATACATGAA+TGG + chr5.2:21528144-21528163 None:intergenic 40.0%
ATCCCAAAAAAGCTTTCGGA+TGG - chr5.2:21528060-21528079 MS.gene027825:CDS 40.0%
GAGCATCCCAAAAAAGCTTT+CGG - chr5.2:21528056-21528075 MS.gene027825:CDS 40.0%
GATAGTGCCATCAACATTCT+TGG + chr5.2:21529149-21529168 None:intergenic 40.0%
GCATATAAAACTTCCCACGT+CGG - chr5.2:21528252-21528271 MS.gene027825:intron 40.0%
TCCCAAAAAAGCTTTCGGAT+GGG - chr5.2:21528061-21528080 MS.gene027825:CDS 40.0%
TGATATTGAGGTTGTTCCAG+TGG - chr5.2:21529948-21529967 MS.gene027825:intron 40.0%
TGGCGTAAAACCTGATATTG+AGG - chr5.2:21529936-21529955 MS.gene027825:intron 40.0%
TGTTGAAGACATGAAGTTGC+TGG - chr5.2:21528974-21528993 MS.gene027825:CDS 40.0%
! ACAATTGGTGGGAGTATGAT+TGG - chr5.2:21529868-21529887 MS.gene027825:intron 40.0%
! CTTGATGACCTAAATTGCAC+TGG + chr5.2:21528449-21528468 None:intergenic 40.0%
! GTGATAAGGCATCAGGAATA+CGG + chr5.2:21529222-21529241 None:intergenic 40.0%
!! ATTCCTTTTCAGGGAAACAG+GGG - chr5.2:21528742-21528761 MS.gene027825:CDS 40.0%
!! ATTGATTTTGCAGCCAAGCA+TGG - chr5.2:21529916-21529935 MS.gene027825:intron 40.0%
!! CATTCCTTTTCAGGGAAACA+GGG - chr5.2:21528741-21528760 MS.gene027825:CDS 40.0%
!! CTCCTTTCAAGTTCTCTAGA+AGG - chr5.2:21528111-21528130 MS.gene027825:CDS 40.0%
!! GTTGATGGCACTATCACATA+TGG - chr5.2:21529154-21529173 MS.gene027825:intron 40.0%
!! TCATTCCTTTTCAGGGAAAC+AGG - chr5.2:21528740-21528759 MS.gene027825:CDS 40.0%
!! TCTTGGTGGATTAGGTCATA+TGG - chr5.2:21529342-21529361 MS.gene027825:intron 40.0%
AAGGAAAAAAGGGACACGCA+AGG + chr5.2:21528215-21528234 None:intergenic 45.0%
ACTACTTACCCAGTAGTTCC+TGG - chr5.2:21528845-21528864 MS.gene027825:CDS 45.0%
ACTCATTCTTAGCCATGTGG+AGG + chr5.2:21528817-21528836 None:intergenic 45.0%
ACTGGATCATCTCTAGCGAA+CGG - chr5.2:21528418-21528437 MS.gene027825:CDS 45.0%
AGCAGCAGCATCAAGTGATA+AGG + chr5.2:21529236-21529255 None:intergenic 45.0%
ATCTCATAAACGATGGGGAC+TGG - chr5.2:21528400-21528419 MS.gene027825:CDS 45.0%
CACATGGCTAAGAATGAGTG+GGG - chr5.2:21528818-21528837 MS.gene027825:CDS 45.0%
CAGGGAGAAAGACAATTGGT+GGG - chr5.2:21529857-21529876 MS.gene027825:intron 45.0%
CATCCTCTAATGCCTTTGCT+TGG - chr5.2:21529668-21529687 MS.gene027825:intron 45.0%
CCACATGGCTAAGAATGAGT+GGG - chr5.2:21528817-21528836 MS.gene027825:CDS 45.0%
CGCGACAACTTTGACATGAA+TGG + chr5.2:21529081-21529100 None:intergenic 45.0%
CTACTTACCCAGTAGTTCCT+GGG - chr5.2:21528846-21528865 MS.gene027825:CDS 45.0%
GACAAACCTGGACTGCATAT+AGG - chr5.2:21529310-21529329 MS.gene027825:CDS 45.0%
GAGTATGATTGGAGGGATGA+AGG - chr5.2:21529879-21529898 MS.gene027825:intron 45.0%
GATGGCACTATCACATATGG+AGG - chr5.2:21529157-21529176 MS.gene027825:intron 45.0%
GCATCGCCAATGTTTGATGA+AGG + chr5.2:21528234-21528253 None:intergenic 45.0%
GTCAGCTCCTAAGTGTTCAA+TGG + chr5.2:21529440-21529459 None:intergenic 45.0%
TATTGGTACGCACCTTCATC+TGG + chr5.2:21529489-21529508 None:intergenic 45.0%
TCCACATGGCTAAGAATGAG+TGG - chr5.2:21528816-21528835 MS.gene027825:CDS 45.0%
TTCAAAGTTGGAGACAGGGT+TGG - chr5.2:21529031-21529050 MS.gene027825:CDS 45.0%
TTCATGTCAAAGTTGTCGCG+AGG - chr5.2:21529081-21529100 MS.gene027825:CDS 45.0%
TTGGCAGGGAGAAAGACAAT+TGG - chr5.2:21529853-21529872 MS.gene027825:intron 45.0%
! AAAGTGTTCGTCGACAACCA+TGG + chr5.2:21529194-21529213 None:intergenic 45.0%
! ATCAGGTTTTACGCCATGCT+TGG + chr5.2:21529932-21529951 None:intergenic 45.0%
! ATGCTCCGTTTCTGGATTTG+AGG + chr5.2:21528042-21528061 None:intergenic 45.0%
! ATTGGTGGGAGTATGATTGG+AGG - chr5.2:21529871-21529890 MS.gene027825:intron 45.0%
! GCATCAAGTGATAAGGCATC+AGG + chr5.2:21529229-21529248 None:intergenic 45.0%
! GTGAAGTTCGCCAAAGCTTT+TGG - chr5.2:21529367-21529386 MS.gene027825:intron 45.0%
! TAGAGTACCCAGGAACTACT+GGG + chr5.2:21528856-21528875 None:intergenic 45.0%
! TTAGAGTACCCAGGAACTAC+TGG + chr5.2:21528857-21528876 None:intergenic 45.0%
! TTGGTGGGAGTATGATTGGA+GGG - chr5.2:21529872-21529891 MS.gene027825:intron 45.0%
!! AGACCAAGCAAAGGCATTAG+AGG + chr5.2:21529674-21529693 None:intergenic 45.0%
!! CAAGCAAAGGCATTAGAGGA+TGG + chr5.2:21529670-21529689 None:intergenic 45.0%
!! CATATAGGTGTGGTTGGTCT+TGG - chr5.2:21529325-21529344 MS.gene027825:CDS 45.0%
!! CGCCAAAGCTTTTGGTGTAA+AGG - chr5.2:21529375-21529394 MS.gene027825:intron 45.0%
!! TAGAGGATGGAGTGCTGAAA+CGG + chr5.2:21529657-21529676 None:intergenic 45.0%
!!! GCCCATCCGAAAGCTTTTTT+GGG + chr5.2:21528065-21528084 None:intergenic 45.0%
!!! TGCCCATCCGAAAGCTTTTT+TGG + chr5.2:21528066-21528085 None:intergenic 45.0%
!!! TTTTTGGGATGCTCCGTTTC+TGG + chr5.2:21528050-21528069 None:intergenic 45.0%
ACACTGTGATCCCAGCACAA+AGG + chr5.2:21529264-21529283 None:intergenic 50.0%
ACCACACCTATATGCAGTCC+AGG + chr5.2:21529319-21529338 None:intergenic 50.0%
ACCTGGACTGCATATAGGTG+TGG - chr5.2:21529315-21529334 MS.gene027825:CDS 50.0%
AGTTGCTGGTGAAGTGACAG+AGG - chr5.2:21528988-21529007 MS.gene027825:CDS 50.0%
CCCACTCATTCTTAGCCATG+TGG + chr5.2:21528820-21528839 None:intergenic 50.0%
CGGAGAGTCCAGTGCAATTT+AGG - chr5.2:21528438-21528457 MS.gene027825:CDS 50.0%
GCAGGGAGAAAGACAATTGG+TGG - chr5.2:21529856-21529875 MS.gene027825:intron 50.0%
GGAAACTCCAAAGGCTTCTC+TGG + chr5.2:21529734-21529753 None:intergenic 50.0%
GGACTGCATATAGGTGTGGT+TGG - chr5.2:21529319-21529338 MS.gene027825:CDS 50.0%
TCGCGAACAAGACCAGATGA+AGG - chr5.2:21529474-21529493 MS.gene027825:intron 50.0%
TCTCCCCTGTTTCCCTGAAA+AGG + chr5.2:21528748-21528767 None:intergenic 50.0%
TTTCAGGGAAACAGGGGAGA+AGG - chr5.2:21528748-21528767 MS.gene027825:CDS 50.0%
! CTTAGGAGCTGACTCATTCG+TGG - chr5.2:21529447-21529466 MS.gene027825:intron 50.0%
!! ATAGGTGTGGTTGGTCTTGG+TGG - chr5.2:21529328-21529347 MS.gene027825:intron 50.0%
!! GCTGTAATGGGCACGTTTGA+TGG - chr5.2:21529620-21529639 MS.gene027825:intron 50.0%
!! GTGGTTGGTCTTGGTGGATT+AGG - chr5.2:21529334-21529353 MS.gene027825:intron 50.0%
AAGCTTTCGGATGGGCAGCT+AGG - chr5.2:21528069-21528088 MS.gene027825:CDS 55.0%
AGCTTTCGGATGGGCAGCTA+GGG - chr5.2:21528070-21528089 MS.gene027825:CDS 55.0%
AGTGACAGAGGTGGGAAGCA+AGG - chr5.2:21529000-21529019 MS.gene027825:CDS 55.0%
ATGTCACTCGGACCTCCACA+TGG - chr5.2:21528802-21528821 MS.gene027825:CDS 55.0%
CTGCTCCTCTCCTTTGTGCT+GGG - chr5.2:21529251-21529270 MS.gene027825:CDS 55.0%
GCTGGTGAAGTGACAGAGGT+GGG - chr5.2:21528992-21529011 MS.gene027825:CDS 55.0%
GGAAACAGGGGAGAAGGATG+TGG - chr5.2:21528754-21528773 MS.gene027825:CDS 55.0%
GTGATCCCAGCACAAAGGAG+AGG + chr5.2:21529259-21529278 None:intergenic 55.0%
TGCTGGTGAAGTGACAGAGG+TGG - chr5.2:21528991-21529010 MS.gene027825:CDS 55.0%
TGGAGCTCCAGAGAAGCCTT+TGG - chr5.2:21529724-21529743 MS.gene027825:intron 55.0%
TGGAGGCTACTCTGACACCA+TGG - chr5.2:21529174-21529193 MS.gene027825:intron 55.0%
!! AGTTGGAGACAGGGTTGGTG+TGG - chr5.2:21529036-21529055 MS.gene027825:CDS 55.0%
!! GTTGGAGACAGGGTTGGTGT+GGG - chr5.2:21529037-21529056 MS.gene027825:CDS 55.0%
!! TTGGAGACAGGGTTGGTGTG+GGG - chr5.2:21529038-21529057 MS.gene027825:CDS 55.0%
CGCGAGTGATGTGCTCGTGA+AGG + chr5.2:21528672-21528691 None:intergenic 60.0%
GCTGCTCCTCTCCTTTGTGC+TGG - chr5.2:21529250-21529269 MS.gene027825:CDS 60.0%
GTTGGTGTGGGGTGCATGGT+GGG - chr5.2:21529049-21529068 MS.gene027825:CDS 60.0%
TGGGCAGCTAGGGACACATC+TGG - chr5.2:21528080-21528099 MS.gene027825:CDS 60.0%
! GGTTGGTGTGGGGTGCATGG+TGG - chr5.2:21529048-21529067 MS.gene027825:CDS 65.0%
!! CAGGGTTGGTGTGGGGTGCA+TGG - chr5.2:21529045-21529064 MS.gene027825:CDS 65.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 21528035 21530065 21528035 ID=MS.gene027825
chr5.2 mRNA 21528035 21530065 21528035 ID=MS.gene027825.t1;Parent=MS.gene027825
chr5.2 exon 21529968 21530065 21529968 ID=MS.gene027825.t1.exon1;Parent=MS.gene027825.t1
chr5.2 CDS 21529968 21530065 21529968 ID=cds.MS.gene027825.t1;Parent=MS.gene027825.t1
chr5.2 exon 21529233 21529346 21529233 ID=MS.gene027825.t1.exon2;Parent=MS.gene027825.t1
chr5.2 CDS 21529233 21529346 21529233 ID=cds.MS.gene027825.t1;Parent=MS.gene027825.t1
chr5.2 exon 21528605 21529118 21528605 ID=MS.gene027825.t1.exon3;Parent=MS.gene027825.t1
chr5.2 CDS 21528605 21529118 21528605 ID=cds.MS.gene027825.t1;Parent=MS.gene027825.t1
chr5.2 exon 21528327 21528480 21528327 ID=MS.gene027825.t1.exon4;Parent=MS.gene027825.t1
chr5.2 CDS 21528327 21528480 21528327 ID=cds.MS.gene027825.t1;Parent=MS.gene027825.t1
chr5.2 exon 21528035 21528240 21528035 ID=MS.gene027825.t1.exon5;Parent=MS.gene027825.t1
chr5.2 CDS 21528035 21528240 21528035 ID=cds.MS.gene027825.t1;Parent=MS.gene027825.t1
Gene Sequence

>MS.gene027825

ATGGCCTCAAATCCAGAAACGGAGCATCCCAAAAAAGCTTTCGGATGGGCAGCTAGGGACACATCTGGAGTCCTTTCTCCTTTCAAGTTCTCTAGAAGGTATGTAACCATTCATGTATTCATGCAGCCTTAGTGAAATTATTATAATGACAACAATAAAAGCTACATTTTACATTATCCTTGCGTGTCCCTTTTTTCCTTCATCAAACATTGGCGATGCATATAAAACTTCCCACGTCGGTTTATATTTTTAGTTTGTTTAAATATAAAGATTAACTAATTGCTTAAACAATGCTAATCAAGGTGGTTTTCACTTGGTATGATGATTTATGTTGTTTGATTGTTGAGTGATAAAGTAAAAGAAACATCTCATAAACGATGGGGACTGGATCATCTCTAGCGAACGGAGAGTCCAGTGCAATTTAGGTCATCAAGAAGTTAAAAGATTTAAAGACAGGGTGTGAATTGTCCCGCTTGAGAAAACATAAGAGATTTTCATTGTAGAGATTCATCCTGAATGTTGATGTGTGATGTAAAATCCTGATTTTTTTTTTTTATGGATAATGCTAATTAAAGATCGAGAAGCATTGTTATAGCGAAAATAACAATTGACATATGATTTATTTTAATTGGCTCCTTCACGAGCACATCACTCGCGAGAAATAACACTGCTCTTTTTTATATATATTTAAATCGATAATATTTTTCATTCCTTTTCAGGGAAACAGGGGAGAAGGATGTGGCATTCAAAGTACTATTTTGCGGTATATGTCACTCGGACCTCCACATGGCTAAGAATGAGTGGGGCAGTACTACTTACCCAGTAGTTCCTGGGTACTCTAACATCAAAAAACAAACCATTGTTTATTTAATTTTCAATGAGTTAAACATTACTCCATCATAAAACAAACTAATTTTTTTGTTTCTTTCTCATCTCATTTGTTGAAGACATGAAGTTGCTGGTGAAGTGACAGAGGTGGGAAGCAAGGTGAAAAACTTCAAAGTTGGAGACAGGGTTGGTGTGGGGTGCATGGTGGGATCCTGCCATTCATGTCAAAGTTGTCGCGAGGATCTCGAAAATTACTGCTCAAAGATGATTCAGACATACAGTTCCAAGAATGTTGATGGCACTATCACATATGGAGGCTACTCTGACACCATGGTTGTCGACGAACACTTTGTTGTCCGTATTCCTGATGCCTTATCACTTGATGCTGCTGCTCCTCTCCTTTGTGCTGGGATCACAGTGTATAGCCCAATGAAACATTTTGGATTAGACAAACCTGGACTGCATATAGGTGTGGTTGGTCTTGGTGGATTAGGTCATATGGCTGTGAAGTTCGCCAAAGCTTTTGGTGTAAAGGTAACTGTGGTAAGTACTTCTCCTAACAAAAAGAAGGAAGCCATTGAACACTTAGGAGCTGACTCATTCGTGGTTAGTCGCGAACAAGACCAGATGAAGGTGCGTACCAATAATTCGCTTTTAATGACTAATTTAGAAACCAAATCTATTTCTATATAGTAATCGATTTATCGACGATCTAAAAAATTGACCTTTAATTCTAATGTTGTTCAATATGTTATAGGCTGTAATGGGCACGTTTGATGGAATCATCGACACCGTTTCAGCACTCCATCCTCTAATGCCTTTGCTTGGTCTATTGAAGAATAATGGTAAACTTGTTATGGTTGGAGCTCCAGAGAAGCCTTTGGAGTTTCCAGTATTTTCTTTGATTGTGGGTAAGCATATGATAAAAATTCTATACAGACTATGTTATATTAAAATATGAAAATTGTTAAATATACATCACATTAATTGTTGGCAGGGAGAAAGACAATTGGTGGGAGTATGATTGGAGGGATGAAGGAAACACAAGAAATGATTGATTTTGCAGCCAAGCATGGCGTAAAACCTGATATTGAGGTTGTTCCAGTGGATTATGTAAATACTGCAATGGAGCGCTTACTCAAAGCTGATGTGAAATATCGATTTGTTATTGACATTGGAAACACAATGAAGGCAAGCTCTTAA
Protein sequence

>MS.gene027825.t1

MASNPETEHPKKAFGWAARDTSGVLSPFKFSRRETGEKDVAFKVLFCGICHSDLHMAKNEWGSTTYPVVPGHEVAGEVTEVGSKVKNFKVGDRVGVGCMVGSCHSCQSCREDLENYCSKMIQTYSSKNVDGTITYGGYSDTMVVDEHFVVRIPDALSLDAAAPLLCAGITVYSPMKHFGLDKPGLHIGVVGLGGLGHMAVKFAKAFGVKVTVVSTSPNKKKEAIEHLGADSFVVSREQDQMKAVMGTFDGIIDTVSALHPLMPLLGLLKNNGKLVMVGAPEKPLEFPVFSLIVGRKTIGGSMIGGMKETQEMIDFAAKHGVKPDIEVVPVDYVNTAMERLLKADVKYRFVIDIGNTMKASS