AlfalfaGEDB | MS.gene58124

M. sativa cultivar XinJiangDaYe / MS.gene58124

KEGG: K00083
GO: GO:0003674 GO:0003824 GO:0005488 GO:0008150 GO:0008152 GO:0008270 GO:0016491 GO:0043167 GO:0043169 GO:0046872 GO:0046914 GO:0055114
PFAM: PF00107.29 PF08240.15

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58124.t1	O82515.1	94.6	373	6	1	1	373	1	359	2.10E-201	711.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58124.t1	O82515	94.6	373	6	1	1	373	1	359	5.1e-204	711.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58124.t1	Q53X16	94.6	373	6	1	1	373	1	359	1.5e-201	711.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050216	MS.gene58124	0.822328	2.51E-53	-1.69E-46
MS.gene050381	MS.gene58124	-0.80974	1.63E-50	-1.69E-46
MS.gene050868	MS.gene58124	0.830378	3.04E-55	-1.69E-46
MS.gene050871	MS.gene58124	0.830982	2.16E-55	-1.69E-46
MS.gene051138	MS.gene58124	-0.820618	6.24E-53	-1.69E-46
MS.gene051268	MS.gene58124	0.827993	1.15E-54	-1.69E-46
MS.gene051509	MS.gene58124	0.811633	6.35E-51	-1.69E-46
MS.gene051874	MS.gene58124	0.827284	1.70E-54	-1.69E-46
MS.gene053420	MS.gene58124	-0.821495	3.92E-53	-1.69E-46
MS.gene05494	MS.gene58124	0.81721	3.71E-52	-1.69E-46
MS.gene055202	MS.gene58124	-0.820765	5.77E-53	-1.69E-46
MS.gene055959	MS.gene58124	-0.821966	3.05E-53	-1.69E-46
MS.gene056422	MS.gene58124	0.826848	2.17E-54	-1.69E-46
MS.gene057086	MS.gene58124	0.8011	1.06E-48	-1.69E-46
MS.gene058099	MS.gene58124	-0.807869	4.09E-50	-1.69E-46
MS.gene058108	MS.gene58124	-0.802799	4.73E-49	-1.69E-46
MS.gene058171	MS.gene58124	-0.825452	4.65E-54	-1.69E-46
MS.gene058173	MS.gene58124	-0.819268	1.27E-52	-1.69E-46
MS.gene058175	MS.gene58124	-0.828896	6.97E-55	-1.69E-46
MS.gene058459	MS.gene58124	0.828434	9.01E-55	-1.69E-46
MS.gene058978	MS.gene58124	-0.801585	8.40E-49	-1.69E-46
MS.gene059037	MS.gene58124	-0.828386	9.25E-55	-1.69E-46
MS.gene059038	MS.gene58124	-0.816916	4.32E-52	-1.69E-46
MS.gene059045	MS.gene58124	-0.827696	1.36E-54	-1.69E-46
MS.gene059129	MS.gene58124	0.833151	6.29E-56	-1.69E-46
MS.gene059865	MS.gene58124	0.801026	1.09E-48	-1.69E-46
MS.gene060372	MS.gene58124	0.802929	4.44E-49	-1.69E-46
MS.gene060778	MS.gene58124	0.808239	3.42E-50	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene58124	MS.gene70270	PPI
MS.gene58124	MS.gene070869	PPI
MS.gene58124	MS.gene068613	PPI
MS.gene58124	MS.gene068614	PPI
MS.gene58124	MS.gene008956	PPI
MS.gene58124	MS.gene027816	PPI
MS.gene58124	MS.gene068619	PPI
MS.gene58124	MS.gene069153	PPI
MS.gene58124	MS.gene070872	PPI
MS.gene58124	MS.gene027823	PPI
MS.gene58124	MS.gene069154	PPI
MS.gene58124	MS.gene027820	PPI
MS.gene58124	MS.gene069147	PPI
MS.gene58124	MS.gene069148	PPI
MS.gene58124	MS.gene28444	PPI
MS.gene58124	MS.gene068612	PPI
MS.gene58124	MS.gene002257	PPI
MS.gene58124	MS.gene027825	PPI
MS.gene58124	MS.gene02214	PPI
MS.gene58124	MS.gene027824	PPI
MS.gene068619	MS.gene58124	PPI
MS.gene58124	MS.gene069152	PPI
MS.gene58124	MS.gene070877	PPI
MS.gene58124	MS.gene90766	PPI
MS.gene58124	MS.gene93004	PPI
MS.gene58124	MS.gene068618	PPI
MS.gene58124	MS.gene036724	PPI
MS.gene58124	MS.gene01230	PPI
MS.gene58124	MS.gene58045	PPI
MS.gene58124	MS.gene068621	PPI
MS.gene068614	MS.gene58124	PPI
MS.gene58124	MS.gene069151	PPI
MS.gene027821	MS.gene58124	PPI
MS.gene58124	MS.gene070879	PPI
MS.gene58124	MS.gene027817	PPI
MS.gene58124	MS.gene030674	PPI
MS.gene58124	MS.gene71681	PPI
MS.gene58124	MS.gene048392	PPI
MS.gene58124	MS.gene070871	PPI
MS.gene58124	MS.gene069146	PPI
MS.gene58124	MS.gene070874	PPI
MS.gene58124	MS.gene46316	PPI
MS.gene58124	MS.gene068611	PPI
MS.gene58124	MS.gene070881	PPI
MS.gene58124	MS.gene027821	PPI
MS.gene58124	MS.gene029589	PPI
MS.gene58124	MS.gene068615	PPI
MS.gene068611	MS.gene58124	PPI
MS.gene58124	MS.gene065692	PPI
MS.gene58124	MS.gene027818	PPI
MS.gene58124	MS.gene068620	PPI
MS.gene58124	MS.gene027819	PPI
MS.gene58124	MS.gene002258	PPI
MS.gene58124	MS.gene069144	PPI
MS.gene58124	MS.gene069145	PPI
MS.gene58124	MS.gene070868	PPI
MS.gene58124	MS.gene070876	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58124.t1	MTR_8g092630	93.370	362	10	1	12	373	69	416	0.0	688
MS.gene58124.t1	MTR_5g031230	63.612	371	120	2	1	370	1	357	4.21e-170	479
MS.gene58124.t1	MTR_5g031190	63.539	373	121	2	1	372	1	359	1.20e-167	473
MS.gene58124.t1	MTR_4g115300	62.803	371	123	2	1	370	1	357	2.17e-167	472
MS.gene58124.t1	MTR_5g031250	61.995	371	126	2	1	370	1	357	4.16e-164	464
MS.gene58124.t1	MTR_5g031390	61.126	373	130	2	1	372	1	359	6.40e-163	461
MS.gene58124.t1	MTR_5g031310	60.714	364	128	2	8	370	9	358	2.96e-162	459
MS.gene58124.t1	MTR_5g031360	61.433	363	125	2	9	370	11	359	2.66e-160	454
MS.gene58124.t1	MTR_5g031210	61.456	371	128	2	1	370	1	357	2.61e-158	449
MS.gene58124.t1	MTR_5g031305	60.165	364	130	2	8	370	9	358	3.32e-158	449
MS.gene58124.t1	MTR_8g068190	62.735	373	124	2	1	372	1	359	1.20e-157	447
MS.gene58124.t1	MTR_5g031300	57.143	364	141	2	8	370	9	358	1.19e-154	439
MS.gene58124.t1	MTR_5g031180	62.198	373	126	2	1	372	1	359	1.95e-153	436
MS.gene58124.t1	MTR_1g025950	57.983	357	134	3	15	369	14	356	1.15e-138	399
MS.gene58124.t1	MTR_5g031430	56.522	368	143	3	4	370	6	357	6.34e-136	392
MS.gene58124.t1	MTR_2g009810	52.925	359	152	4	14	370	62	405	4.58e-116	343
MS.gene58124.t1	MTR_1g107425	48.315	356	169	2	15	369	13	354	2.54e-112	332
MS.gene58124.t1	MTR_5g031250	60.392	255	86	2	1	254	1	241	8.85e-106	311
MS.gene58124.t1	MTR_1g107425	48.630	292	134	3	15	305	13	289	9.77e-89	270
MS.gene58124.t1	MTR_2g009820	50.607	247	115	4	130	370	8	253	4.03e-63	202
MS.gene58124.t1	MTR_5g031290	64.602	113	40	0	260	372	80	192	9.07e-47	158
MS.gene58124.t1	MTR_5g031290	59.184	98	25	2	1	97	1	84	5.62e-30	114
MS.gene58124.t1	MTR_8g095220	28.679	265	158	10	56	292	83	344	3.70e-22	97.4
MS.gene58124.t1	MTR_8g095220	29.464	224	130	8	53	251	80	300	8.06e-21	92.0
MS.gene58124.t1	MTR_0001s0660	28.664	307	182	12	53	331	36	333	3.40e-17	83.2
MS.gene58124.t1	MTR_6g465010	24.275	276	187	7	44	306	16	282	3.07e-15	76.6
MS.gene58124.t1	MTR_6g466320	24.444	270	182	7	50	306	38	298	3.58e-15	76.3
MS.gene58124.t1	MTR_6g465010	24.444	270	182	7	50	306	38	298	3.58e-15	76.3
MS.gene58124.t1	MTR_7g099040	25.664	339	211	12	52	355	37	369	2.63e-14	73.9
MS.gene58124.t1	MTR_1g069430	25.074	339	211	12	53	355	36	367	3.35e-13	70.5
MS.gene58124.t1	MTR_3g099460	26.183	317	173	12	53	325	44	343	2.16e-12	68.2
MS.gene58124.t1	MTR_3g099460	26.183	317	173	12	53	325	37	336	2.52e-12	68.2
MS.gene58124.t1	MTR_3g079180	25.839	298	186	13	53	320	41	333	3.50e-12	67.4
MS.gene58124.t1	MTR_8g064610	27.083	240	139	7	51	279	103	317	6.61e-12	66.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58124.t1	AT4G39330	73.656	372	83	2	1	371	1	358	0.0	548
MS.gene58124.t1	AT2G21730	69.337	362	94	3	12	370	6	353	0.0	508
MS.gene58124.t1	AT2G21890	69.529	361	94	3	12	370	6	352	0.0	508
MS.gene58124.t1	AT4G39330	71.429	315	75	2	1	314	1	301	1.06e-157	445
MS.gene58124.t1	AT4G37970	58.108	370	140	2	4	372	5	360	6.92e-156	443
MS.gene58124.t1	AT4G37990	63.636	363	117	2	9	370	5	353	3.29e-153	436
MS.gene58124.t1	AT4G37980	62.259	363	122	2	9	370	5	353	1.32e-151	432
MS.gene58124.t1	AT4G37970	57.705	305	114	2	4	307	5	295	4.42e-122	355
MS.gene58124.t1	AT1G72680	50.843	356	159	3	16	370	14	354	5.17e-116	341
MS.gene58124.t1	AT4G37980	59.736	303	107	2	9	310	5	293	2.02e-115	338
MS.gene58124.t1	AT3G19450	48.468	359	170	2	12	369	10	354	6.83e-109	323
MS.gene58124.t1	AT4G34230	48.760	363	171	2	8	369	5	353	2.65e-104	311
MS.gene58124.t1	AT4G34230	48.485	363	172	2	8	369	5	353	2.23e-103	309
MS.gene58124.t1	AT4G37970	56.078	255	97	2	4	257	5	245	1.42e-96	288
MS.gene58124.t1	AT4G37970	56.522	253	95	2	4	255	5	243	3.25e-96	287
MS.gene58124.t1	AT5G63620	25.926	351	219	13	52	366	81	426	1.04e-23	102
MS.gene58124.t1	AT5G63620	25.926	351	219	13	52	366	81	426	1.80e-23	101
MS.gene58124.t1	AT5G63620	29.907	214	124	7	52	242	81	291	1.93e-21	94.0
MS.gene58124.t1	AT5G43940	25.926	351	218	11	53	368	36	379	6.84e-19	87.8
MS.gene58124.t1	AT5G43940	25.926	351	218	11	53	368	48	391	7.37e-19	87.8
MS.gene58124.t1	AT1G22430	26.979	341	205	13	53	355	42	376	3.12e-18	85.9
MS.gene58124.t1	AT1G22430	26.979	341	205	13	53	355	42	376	3.12e-18	85.9
MS.gene58124.t1	AT5G51970	26.667	270	176	7	50	306	40	300	5.65e-18	84.7
MS.gene58124.t1	AT5G51970	26.667	270	176	7	50	306	40	300	5.65e-18	84.7
MS.gene58124.t1	AT4G22110	26.549	339	205	13	53	353	43	375	9.99e-17	81.3
MS.gene58124.t1	AT4G22110	26.549	339	205	13	53	353	43	375	9.99e-17	81.3
MS.gene58124.t1	AT1G64710	24.706	340	214	14	53	355	13	347	1.13e-15	77.8
MS.gene58124.t1	AT1G64710	24.706	340	214	14	53	355	36	370	1.96e-15	77.4
MS.gene58124.t1	AT1G22430	27.723	202	120	5	53	232	42	239	2.02e-14	73.9
MS.gene58124.t1	AT1G22430	27.723	202	120	5	53	232	42	239	2.60e-14	73.2
MS.gene58124.t1	AT1G22430	27.723	202	120	5	53	232	42	239	2.60e-14	73.2
MS.gene58124.t1	AT5G42250	23.512	336	219	10	53	353	45	377	5.35e-14	73.2
MS.gene58124.t1	AT1G22440	25.497	302	183	11	53	320	40	333	2.61e-13	71.2
MS.gene58124.t1	AT4G22110	27.528	178	104	4	53	209	43	216	6.84e-13	68.9

Find 103 sgRNAs with CRISPR-Local

Find 237 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATCAAATTCGGTAAAGCATT+TGG	0.245686	4.2:+9638801	MS.gene58124:CDS
ACTACTTACCCTGTTGTTCC+TGG	0.250937	4.2:+9636905	MS.gene58124:CDS
ACCTATAACTCTCCTTATAA+AGG	0.251163	4.2:+9638573	MS.gene58124:CDS
TTGAGTGGGAGTTCGGTTTC+GGG	0.283001	4.2:-9636626	None:intergenic
TTTGAGTGGGAGTTCGGTTT+CGG	0.285055	4.2:-9636627	None:intergenic
TCTGTCTTTCCATTAGTTGC+AGG	0.301516	4.2:+9639706	MS.gene58124:CDS
ATGGCCTCAGTTTCCTTGTT+TGG	0.302289	4.2:-9638852	None:intergenic
GTGGCAGGGCTTGGTGGGTT+AGG	0.307365	4.2:+9638768	MS.gene58124:CDS
TGAGAATTGCAATCAAGATT+TGG	0.319987	4.2:+9638524	MS.gene58124:CDS
GAACTCCCACTCAAAGCTTT+TGG	0.323434	4.2:+9636635	MS.gene58124:CDS
TAGGTTCGTGTCCCTTTATA+AGG	0.354122	4.2:-9638585	None:intergenic
TTGGGAGTGGCAGGGCTTGG+TGG	0.355141	4.2:+9638762	MS.gene58124:CDS
TTATAGGTAAATACAGGTTT+AGG	0.360036	4.2:-9638558	None:intergenic
CCCTTTATAAGGAGAGTTAT+AGG	0.360288	4.2:-9638574	None:intergenic
GTAGACCAAGCAGTGGCATC+AGG	0.373980	4.2:-9639627	None:intergenic
GATGGTTTACCTGCAACTAA+TGG	0.378875	4.2:-9639715	None:intergenic
TATAGCCCGATGAAGTATTA+TGG	0.379807	4.2:+9638717	MS.gene58124:CDS
GCCACTCCCAAATGTTTGCC+TGG	0.380570	4.2:-9638750	None:intergenic
CCTATAACTCTCCTTATAAA+GGG	0.390671	4.2:+9638574	MS.gene58124:CDS
GGCAAACATTTGGGAGTGGC+AGG	0.391166	4.2:+9638753	MS.gene58124:CDS
ACAAAGCTGATGTGAAATAT+CGG	0.393376	4.2:+9640093	MS.gene58124:CDS
CAGACATACCCAGGAACAAC+AGG	0.394883	4.2:-9636914	None:intergenic
CTCCCTTTCATTTCTCTCGA+AGG	0.396574	4.2:+9636690	MS.gene58124:CDS
ACTGAAGTTGAATGGGAAAC+TGG	0.399835	4.2:+9639648	MS.gene58124:CDS
GACTGAACCAGGCAAACATT+TGG	0.404496	4.2:+9638743	MS.gene58124:CDS
TCAAATTCGGTAAAGCATTT+GGG	0.405047	4.2:+9638802	MS.gene58124:CDS
ACTGCAGGACGGAAGCTTAT+CGG	0.411894	4.2:+9639945	MS.gene58124:intron
CATTTGGGAGTGGCAGGGCT+TGG	0.412676	4.2:+9638759	MS.gene58124:CDS
GGCCATGTTGCAATCAAATT+CGG	0.413822	4.2:+9638789	MS.gene58124:CDS
GGAGAGTTATAGGTAAATAC+AGG	0.414003	4.2:-9638564	None:intergenic
CTACTTACCCTGTTGTTCCT+GGG	0.415236	4.2:+9636906	MS.gene58124:CDS
GTGCTCCACTTCTGTGTGCC+GGG	0.418161	4.2:+9638685	MS.gene58124:CDS
AAAAGCTTTGAGTGGGAGTT+CGG	0.429852	4.2:-9636633	None:intergenic
GGTGCTCCACTTCTGTGTGC+CGG	0.433744	4.2:+9638684	MS.gene58124:CDS
ACTGAACCAGGCAAACATTT+GGG	0.439930	4.2:+9638744	MS.gene58124:CDS
CATTCCCTGATGCCACTGCT+TGG	0.448606	4.2:+9639622	MS.gene58124:CDS
TGGGCTGCTAGAGACACTTC+TGG	0.465043	4.2:+9636659	MS.gene58124:CDS
ACACACTCTCAAGAACGATT+GGG	0.475053	4.2:+9636877	MS.gene58124:CDS
AAGTGGAGCACCAGCGTCAA+GGG	0.477411	4.2:-9638673	None:intergenic
CGGTAAAGCATTTGGGCTGA+AGG	0.479081	4.2:+9638809	MS.gene58124:CDS
TACACACTCTCAAGAACGAT+TGG	0.482360	4.2:+9636876	MS.gene58124:CDS
CTCAGTTTCCTTGTTTGGAG+AGG	0.482678	4.2:-9638847	None:intergenic
GCGTCAAGGGGTAAGTTGTC+AGG	0.483209	4.2:-9638660	None:intergenic
TGGGAGTGGCAGGGCTTGGT+GGG	0.488091	4.2:+9638763	MS.gene58124:CDS
TCATACCATAATACTTCATC+GGG	0.495385	4.2:-9638722	None:intergenic
GAGAGCTCAAGGGGCTTGCT+AGG	0.495481	4.2:-9639685	None:intergenic
CTTGGTCTACTGAAGTTGAA+TGG	0.496517	4.2:+9639640	MS.gene58124:CDS
GTCATACCATAATACTTCAT+CGG	0.503227	4.2:-9638723	None:intergenic
GTTGGCGTTGTGACAAAAGT+TGG	0.504641	4.2:+9638429	MS.gene58124:CDS
AACCTTCGAGAGAAATGAAA+GGG	0.510126	4.2:-9636692	None:intergenic
TTGGTCTACTGAAGTTGAAT+GGG	0.524981	4.2:+9639641	MS.gene58124:CDS
AGCTTTGTGAAGCCTTTCCA+TGG	0.525881	4.2:-9640078	None:intergenic
TCTCAGGGTCTTTGGAAACA+AGG	0.527412	4.2:-9638899	None:intergenic
CGGGCTATACACGGTGATCC+CGG	0.532660	4.2:-9638703	None:intergenic
ATCAGCACCAAGTTTATCAA+TGG	0.533531	4.2:-9638871	None:intergenic
GCATGAAATCAACACGGCCA+TGG	0.534341	4.2:+9640061	MS.gene58124:CDS
ATGGAAAGACAGAGAGCTCA+AGG	0.539760	4.2:-9639696	None:intergenic
GTGATCCCGGCACACAGAAG+TGG	0.541709	4.2:-9638690	None:intergenic
TATAAAGGGACACGAACCTA+TGG	0.552541	4.2:+9638588	MS.gene58124:CDS
AAATCAACACGGCCATGGAA+AGG	0.552965	4.2:+9640066	MS.gene58124:CDS
CTTATCGGAGGGAGTAACAT+TGG	0.554132	4.2:+9639960	MS.gene58124:CDS
ACTGAGGCCATTGATAAACT+TGG	0.554500	4.2:+9638864	MS.gene58124:CDS
AATGGGAAACTGGTCACTGT+AGG	0.556394	4.2:+9639658	MS.gene58124:CDS
CAAAGACCCTGAGAAAATGA+AGG	0.559572	4.2:+9638908	MS.gene58124:CDS
GAACCTTCGAGAGAAATGAA+AGG	0.562759	4.2:-9636693	None:intergenic
TGGAGGGATGAAAGAGACTC+AGG	0.563071	4.2:+9639980	MS.gene58124:CDS
GAAGTGGAGCACCAGCGTCA+AGG	0.573782	4.2:-9638674	None:intergenic
CTACAAACTTGAGAAGGAGT+TGG	0.582204	4.2:-9640132	None:intergenic
TATTAGTACCTCTCCAAACA+AGG	0.586732	4.2:+9638839	MS.gene58124:CDS
CACACTCTCAAGAACGATTG+GGG	0.590819	4.2:+9636878	MS.gene58124:CDS
GCAGGCTGCTATGGGAACCA+TGG	0.590820	4.2:+9639570	MS.gene58124:intron
TATTATGGTATGACTGAACC+AGG	0.592818	4.2:+9638732	MS.gene58124:CDS
TGGCCTCTACAAACTTGAGA+AGG	0.595694	4.2:-9640138	None:intergenic
GCAAACATTTGGGAGTGGCA+GGG	0.595862	4.2:+9638754	MS.gene58124:CDS
CCCAACCAAAAGCTTTGAGT+GGG	0.597594	4.2:-9636640	None:intergenic
TACCGAATTTGATTGCAACA+TGG	0.599803	4.2:-9638791	None:intergenic
ACCAGGCAAACATTTGGGAG+TGG	0.602207	4.2:+9638749	MS.gene58124:CDS
AAAATCAGAATAGCCACCAT+AGG	0.602620	4.2:-9638604	None:intergenic
TGGAAAGACAGAGAGCTCAA+GGG	0.604195	4.2:-9639695	None:intergenic
TCGGAGGGAGTAACATTGGA+GGG	0.606287	4.2:+9639964	MS.gene58124:CDS
TAGACCAAGCAGTGGCATCA+GGG	0.607212	4.2:-9639626	None:intergenic
ATCGGAGGGAGTAACATTGG+AGG	0.607417	4.2:+9639963	MS.gene58124:CDS
ACTCCTTCTCAAGTTTGTAG+AGG	0.609994	4.2:+9640135	MS.gene58124:CDS
GTGGGTGATAACGTTGGTGT+TGG	0.610605	4.2:+9638474	MS.gene58124:CDS
GAAACTGAACTACATACCGC+TGG	0.611677	4.2:-9638638	None:intergenic
GCCCAACCAAAAGCTTTGAG+TGG	0.615088	4.2:-9636641	None:intergenic
GACAACTTACCCCTTGACGC+TGG	0.625682	4.2:+9638663	MS.gene58124:CDS
GGAAAGACAGAGAGCTCAAG+GGG	0.626737	4.2:-9639694	None:intergenic
AGTGTGAAAATTCTATATTG+TGG	0.629238	4.2:+9636836	MS.gene58124:CDS
CTCTCCAAACAAGGAAACTG+AGG	0.630189	4.2:+9638848	MS.gene58124:CDS
ATGGGAAACTGGTCACTGTA+GGG	0.633427	4.2:+9639659	MS.gene58124:CDS
AGTGGAGCACCAGCGTCAAG+GGG	0.637977	4.2:-9638672	None:intergenic
AAAGGGACACGAACCTATGG+TGG	0.638008	4.2:+9638591	MS.gene58124:CDS
TGTGTCTATGATGTAGTCCA+TGG	0.640415	4.2:-9639587	None:intergenic
CAGGAGATGCTCGATTTCTG+CGG	0.641521	4.2:+9639999	MS.gene58124:CDS
ATACTTCATCGGGCTATACA+CGG	0.644181	4.2:-9638712	None:intergenic
TTCAGAGTGGGTGATAACGT+TGG	0.661444	4.2:+9638468	MS.gene58124:CDS
GCAGGACGGAAGCTTATCGG+AGG	0.666483	4.2:+9639948	MS.gene58124:intron
CAGGACGGAAGCTTATCGGA+GGG	0.671442	4.2:+9639949	MS.gene58124:intron
ATATCGGTTTGTGATTGATG+TGG	0.686371	4.2:+9640109	MS.gene58124:CDS
AACTTCAGTAGACCAAGCAG+TGG	0.689529	4.2:-9639634	None:intergenic
AGACATACCCAGGAACAACA+GGG	0.709790	4.2:-9636913	None:intergenic
TAAGATGCATGAAATCAACA+CGG	0.753982	4.2:+9640055	MS.gene58124:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAAATATATATTCAGAAATA+AGG	-	chr4.2:9639248-9639267	None:intergenic	10.0%
!!!	AAATTTAAAATATTTTGTGT+AGG	-	chr4.2:9639119-9639138	None:intergenic	10.0%
!!	TAGAATAATATAGTAATTGT+TGG	+	chr4.2:9639150-9639169	MS.gene58124:intron	15.0%
!!	TATTTGATTGTTAAATTAGT+TGG	+	chr4.2:9638243-9638262	MS.gene58124:intron	15.0%
!!!	TTTTCATATTTGTTTTCTTT+TGG	+	chr4.2:9639466-9639485	MS.gene58124:intron	15.0%
!!!	TTTTCATTCTTTTTCATTAA+TGG	+	chr4.2:9637962-9637981	MS.gene58124:intron	15.0%
!!	ATCCATAGTTTACTTATAAA+TGG	+	chr4.2:9639046-9639065	MS.gene58124:intron	20.0%
!!	ATTTAATTATCTGTTCGTAA+GGG	-	chr4.2:9637613-9637632	None:intergenic	20.0%
!!	TATTTAATTATCTGTTCGTA+AGG	-	chr4.2:9637614-9637633	None:intergenic	20.0%
!!	TCCATAGTTTACTTATAAAT+GGG	+	chr4.2:9639047-9639066	MS.gene58124:intron	20.0%
!!	TGAAAATTTGGATAATTCAT+TGG	-	chr4.2:9639909-9639928	None:intergenic	20.0%
!!	TGTTAAACATGATTAAAAAC+AGG	-	chr4.2:9637662-9637681	None:intergenic	20.0%
!!	TGTTGAATTGAATAGAAAAA+TGG	+	chr4.2:9637068-9637087	MS.gene58124:intron	20.0%
!!	TTTGTTTATATACTCATTGA+CGG	-	chr4.2:9639321-9639340	None:intergenic	20.0%
!!!	GTTTAAATACAATTTTATGC+AGG	+	chr4.2:9639552-9639571	MS.gene58124:intron	20.0%
!!!	TAATTCACTTTATTTACCTT+TGG	+	chr4.2:9637289-9637308	MS.gene58124:intron	20.0%
!!!	TCTTTTTCATTAATGGTTTA+TGG	+	chr4.2:9637969-9637988	MS.gene58124:intron	20.0%
!!!	TTTATTTTTTGTAGGGAAAA+TGG	+	chr4.2:9636800-9636819	MS.gene58124:intron	20.0%
!!!	TTTTCAAATTATTAGTGTGT+CGG	-	chr4.2:9637777-9637796	None:intergenic	20.0%
!	AAACATACACATAATTCCAA+AGG	-	chr4.2:9637308-9637327	None:intergenic	25.0%
!	AAACATGATTAAAAACAGGT+AGG	-	chr4.2:9637658-9637677	None:intergenic	25.0%
!	AAACTATCAATCAATAGTCA+AGG	-	chr4.2:9638277-9638296	None:intergenic	25.0%
!	AATGTGAAAAAATTCAGAGT+GGG	+	chr4.2:9638456-9638475	MS.gene58124:CDS	25.0%
!	AATTGACAGAGAATTATTGA+TGG	-	chr4.2:9639736-9639755	None:intergenic	25.0%
!	ACATTAGACATGTGAAAATT+TGG	-	chr4.2:9639921-9639940	None:intergenic	25.0%
!	AGCTTATGAAAATTCTCATA+AGG	-	chr4.2:9637401-9637420	None:intergenic	25.0%
!	AGTGTGAAAATTCTATATTG+TGG	+	chr4.2:9636836-9636855	MS.gene58124:CDS	25.0%
!	CATACTTGTCTATTAGAATT+TGG	+	chr4.2:9637255-9637274	MS.gene58124:intron	25.0%
!	CATTAATCCTAGTAAACTAT+TGG	+	chr4.2:9638009-9638028	MS.gene58124:intron	25.0%
!	CTTGCTTATAAGTTATATGA+AGG	+	chr4.2:9638338-9638357	MS.gene58124:intron	25.0%
!	TAATCATGTTTAACACACTT+AGG	+	chr4.2:9637667-9637686	MS.gene58124:intron	25.0%
!	TAGGTGGTTATTCATATAAT+AGG	-	chr4.2:9637639-9637658	None:intergenic	25.0%
!	TATTCAATTCAACAGAAAGA+CGG	-	chr4.2:9637062-9637081	None:intergenic	25.0%
!	TTAAGTGTAATTACAAGTGT+CGG	+	chr4.2:9637807-9637826	MS.gene58124:intron	25.0%
!	TTATAGGTAAATACAGGTTT+AGG	-	chr4.2:9638561-9638580	None:intergenic	25.0%
!!	AGACACATTATGTAATTTTG+AGG	-	chr4.2:9638044-9638063	None:intergenic	25.0%
!!	TTTGGAATTATGTGTATGTT+TGG	+	chr4.2:9637307-9637326	MS.gene58124:intron	25.0%
!!!	AACAAAAACAATGGATTGAT+TGG	+	chr4.2:9639502-9639521	MS.gene58124:intron	25.0%
!!!	ACCTCTAATTTTAATTTGAG+GGG	-	chr4.2:9637121-9637140	None:intergenic	25.0%
!!!	ACTTTTGAGGATTCAAATTA+AGG	-	chr4.2:9637703-9637722	None:intergenic	25.0%
!!!	ATCCAGAAATTTTGAAAACT+GGG	-	chr4.2:9639298-9639317	None:intergenic	25.0%
!!!	ATGTAATTTTGAGGTTACAT+GGG	-	chr4.2:9638035-9638054	None:intergenic	25.0%
!!!	CACCTCTAATTTTAATTTGA+GGG	-	chr4.2:9637122-9637141	None:intergenic	25.0%
!!!	TATGTAATTTTGAGGTTACA+TGG	-	chr4.2:9638036-9638055	None:intergenic	25.0%
	ACAAAGCTGATGTGAAATAT+CGG	+	chr4.2:9640093-9640112	MS.gene58124:CDS	30.0%
	ACCCCTCAAATTAAAATTAG+AGG	+	chr4.2:9637117-9637136	MS.gene58124:intron	30.0%
	ACCTATAACTCTCCTTATAA+AGG	+	chr4.2:9638573-9638592	MS.gene58124:CDS	30.0%
	ACGAAATAAGAGTAAAACAG+CGG	-	chr4.2:9638989-9639008	None:intergenic	30.0%
	AGTTATATGAAGGTAAAACC+AGG	+	chr4.2:9638348-9638367	MS.gene58124:intron	30.0%
	CAAATTAAAATTAGAGGTGG+AGG	+	chr4.2:9637123-9637142	MS.gene58124:intron	30.0%
	CAATGTGAAAAAATTCAGAG+TGG	+	chr4.2:9638455-9638474	MS.gene58124:CDS	30.0%
	CCTATAACTCTCCTTATAAA+GGG	+	chr4.2:9638574-9638593	MS.gene58124:CDS	30.0%
	CCTCAAATTAAAATTAGAGG+TGG	+	chr4.2:9637120-9637139	MS.gene58124:intron	30.0%
	CCTGAGTTTGAATAATAGAA+AGG	+	chr4.2:9639356-9639375	MS.gene58124:intron	30.0%
	CCTTTCTATTATTCAAACTC+AGG	-	chr4.2:9639359-9639378	None:intergenic	30.0%
	CTTTGTGCAATAGTCAATAT+TGG	+	chr4.2:9638309-9638328	MS.gene58124:intron	30.0%
	GAGAACACATGATTTGATTA+TGG	-	chr4.2:9639437-9639456	None:intergenic	30.0%
	GAGAATAAGTGTAGGTTATT+TGG	-	chr4.2:9639184-9639203	None:intergenic	30.0%
	GCCCATTTATAAGTAAACTA+TGG	-	chr4.2:9639051-9639070	None:intergenic	30.0%
	GGTTGTTGTAACAAAAACAA+TGG	+	chr4.2:9639493-9639512	MS.gene58124:intron	30.0%
	GTCATACCATAATACTTCAT+CGG	-	chr4.2:9638726-9638745	None:intergenic	30.0%
	GTTCTATATAGGAGTTGTTA+TGG	+	chr4.2:9639015-9639034	MS.gene58124:intron	30.0%
	TAAGATGCATGAAATCAACA+CGG	+	chr4.2:9640055-9640074	MS.gene58124:CDS	30.0%
	TAGTTTACTTATAAATGGGC+AGG	+	chr4.2:9639051-9639070	MS.gene58124:intron	30.0%
	TCATACCATAATACTTCATC+GGG	-	chr4.2:9638725-9638744	None:intergenic	30.0%
	TGAGAATTGCAATCAAGATT+TGG	+	chr4.2:9638524-9638543	MS.gene58124:CDS	30.0%
	TGAGCATGTATAAATGTTTC+AGG	-	chr4.2:9639831-9639850	None:intergenic	30.0%
	TTCGTCATACAGTTCTATAT+AGG	+	chr4.2:9639004-9639023	MS.gene58124:intron	30.0%
!	ATCAAATTCGGTAAAGCATT+TGG	+	chr4.2:9638801-9638820	MS.gene58124:CDS	30.0%
!	ATCTTACCTTCATTTTCTCA+GGG	-	chr4.2:9638917-9638936	None:intergenic	30.0%
!	GATGATATGTATGCGATTTT+TGG	+	chr4.2:9638143-9638162	MS.gene58124:intron	30.0%
!	GGGGCACTATATATATTAAT+TGG	-	chr4.2:9639277-9639296	None:intergenic	30.0%
!	TAAATGTTTCAGGTCAGATT+TGG	-	chr4.2:9639821-9639840	None:intergenic	30.0%
!	TATCTTACCTTCATTTTCTC+AGG	-	chr4.2:9638918-9638937	None:intergenic	30.0%
!	TCAAATTCGGTAAAGCATTT+GGG	+	chr4.2:9638802-9638821	MS.gene58124:CDS	30.0%
!!	GATCCAGAAATTTTGAAAAC+TGG	-	chr4.2:9639299-9639318	None:intergenic	30.0%
!!	TATTTGTTTTCTTTTGGACC+AGG	+	chr4.2:9639472-9639491	MS.gene58124:intron	30.0%
!!	TCCAGAAATTTTGAAAACTG+GGG	-	chr4.2:9639297-9639316	None:intergenic	30.0%
!!	TTTGCAACTATTCACTTTTG+AGG	-	chr4.2:9637716-9637735	None:intergenic	30.0%
!!!	CAGGTTGGTTTTAAAGTTAA+TGG	-	chr4.2:9638946-9638965	None:intergenic	30.0%
!!!	CCACCTCTAATTTTAATTTG+AGG	-	chr4.2:9637123-9637142	None:intergenic	30.0%
!!!	TGGTTTTAAAGTTAATGGTG+TGG	-	chr4.2:9638941-9638960	None:intergenic	30.0%
!!!	TTAACTTGCATTGACTTTTG+TGG	+	chr4.2:9638383-9638402	MS.gene58124:intron	30.0%
!!!	TTGTTTTTGTTACAACAACC+TGG	-	chr4.2:9639493-9639512	None:intergenic	30.0%
	AAAATCAGAATAGCCACCAT+AGG	-	chr4.2:9638607-9638626	None:intergenic	35.0%
	AAAGAAAAACAGACATACCC+AGG	-	chr4.2:9636926-9636945	None:intergenic	35.0%
	AACCTTCGAGAGAAATGAAA+GGG	-	chr4.2:9636695-9636714	None:intergenic	35.0%
	AATTTGAGGGGTAAAGAACT+GGG	-	chr4.2:9637109-9637128	None:intergenic	35.0%
	ATCAGCACCAAGTTTATCAA+TGG	-	chr4.2:9638874-9638893	None:intergenic	35.0%
	CATGATTAAAAACAGGTAGG+TGG	-	chr4.2:9637655-9637674	None:intergenic	35.0%
	CCCTTTATAAGGAGAGTTAT+AGG	-	chr4.2:9638577-9638596	None:intergenic	35.0%
	CTGTGTTACAAATGAAGTCT+AGG	+	chr4.2:9638062-9638081	MS.gene58124:intron	35.0%
	GGAGAGTTATAGGTAAATAC+AGG	-	chr4.2:9638567-9638586	None:intergenic	35.0%
	GTATGACATAATCATCAAGC+AGG	-	chr4.2:9637230-9637249	None:intergenic	35.0%
	GTCAAGTGTGATTTAAGTGT+GGG	-	chr4.2:9639213-9639232	None:intergenic	35.0%
	TAATTTGAGGGGTAAAGAAC+TGG	-	chr4.2:9637110-9637129	None:intergenic	35.0%
	TACCGAATTTGATTGCAACA+TGG	-	chr4.2:9638794-9638813	None:intergenic	35.0%
	TACTTTGCAACTATGAAGCA+CGG	-	chr4.2:9637891-9637910	None:intergenic	35.0%
	TATTAGTACCTCTCCAAACA+AGG	+	chr4.2:9638839-9638858	MS.gene58124:CDS	35.0%
	TATTATGGTATGACTGAACC+AGG	+	chr4.2:9638732-9638751	MS.gene58124:CDS	35.0%
	TGTCAAGTGTGATTTAAGTG+TGG	-	chr4.2:9639214-9639233	None:intergenic	35.0%
	TTGGTCTACTGAAGTTGAAT+GGG	+	chr4.2:9639641-9639660	MS.gene58124:CDS	35.0%
!	AATTTTATGCAGGCTGCTAT+GGG	+	chr4.2:9639562-9639581	MS.gene58124:intron	35.0%
!	ATTGATAGTTTATGCGTGCA+TGG	+	chr4.2:9638286-9638305	MS.gene58124:intron	35.0%
!	TATAGCCCGATGAAGTATTA+TGG	+	chr4.2:9638717-9638736	MS.gene58124:CDS	35.0%
!	TCACTGCAGTTTTCTATCTT+TGG	+	chr4.2:9638206-9638225	MS.gene58124:intron	35.0%
!	TTTTGCAGGCATGAAATTGT+TGG	+	chr4.2:9638411-9638430	MS.gene58124:intron	35.0%
!!	ATATCGGTTTGTGATTGATG+TGG	+	chr4.2:9640109-9640128	MS.gene58124:CDS	35.0%
!!	CCAGAAATTTTGAAAACTGG+GGG	-	chr4.2:9639296-9639315	None:intergenic	35.0%
!!	GATATGTATGCGATTTTTGG+TGG	+	chr4.2:9638146-9638165	MS.gene58124:intron	35.0%
	AAAACCAACCTGTTTCGTCT+AGG	+	chr4.2:9638954-9638973	MS.gene58124:intron	40.0%
	AAAGCTATGAAGCACGGATA+CGG	+	chr4.2:9637733-9637752	MS.gene58124:intron	40.0%
	AAAGTTTCCGCTTCTAGATC+GGG	-	chr4.2:9638187-9638206	None:intergenic	40.0%
	ACACACTCTCAAGAACGATT+GGG	+	chr4.2:9636877-9636896	MS.gene58124:CDS	40.0%
	ACATGGGCCAATAGTTTACT+AGG	-	chr4.2:9638019-9638038	None:intergenic	40.0%
	ACTGAACCAGGCAAACATTT+GGG	+	chr4.2:9638744-9638763	MS.gene58124:CDS	40.0%
	ACTGAAGTTGAATGGGAAAC+TGG	+	chr4.2:9639648-9639667	MS.gene58124:CDS	40.0%
	ACTGAGGCCATTGATAAACT+TGG	+	chr4.2:9638864-9638883	MS.gene58124:CDS	40.0%
	AGGTAAAACCAGGACATCTT+TGG	+	chr4.2:9638358-9638377	MS.gene58124:intron	40.0%
	AGTTAATCCCAAAGATGTCC+TGG	-	chr4.2:9638369-9638388	None:intergenic	40.0%
	AGTTGCAAAGCTATGAAGCA+CGG	+	chr4.2:9637727-9637746	MS.gene58124:intron	40.0%
	ATAACCTAGACGAAACAGGT+TGG	-	chr4.2:9638961-9638980	None:intergenic	40.0%
	ATACTTCATCGGGCTATACA+CGG	-	chr4.2:9638715-9638734	None:intergenic	40.0%
	ATGTCTAATGTCTTGACTGC+AGG	+	chr4.2:9639930-9639949	MS.gene58124:intron	40.0%
	CAAAGACCCTGAGAAAATGA+AGG	+	chr4.2:9638908-9638927	MS.gene58124:CDS	40.0%
	CAATATTCCCGATCTAGAAG+CGG	+	chr4.2:9638177-9638196	MS.gene58124:intron	40.0%
	CACTGCAAAGACTGGAAATA+AGG	+	chr4.2:9638121-9638140	MS.gene58124:intron	40.0%
	CTACAAACTTGAGAAGGAGT+TGG	-	chr4.2:9640135-9640154	None:intergenic	40.0%
	CTTGGTCTACTGAAGTTGAA+TGG	+	chr4.2:9639640-9639659	MS.gene58124:CDS	40.0%
	GAAAGTTTCCGCTTCTAGAT+CGG	-	chr4.2:9638188-9638207	None:intergenic	40.0%
	GAACCTTCGAGAGAAATGAA+AGG	-	chr4.2:9636696-9636715	None:intergenic	40.0%
	GATGGTTTACCTGCAACTAA+TGG	-	chr4.2:9639718-9639737	None:intergenic	40.0%
	GGAAGTAACAGACTCATTCT+TGG	-	chr4.2:9639800-9639819	None:intergenic	40.0%
	GGCAAAGAGAGAATAAGTGT+AGG	-	chr4.2:9639192-9639211	None:intergenic	40.0%
	GGCCATGTTGCAATCAAATT+CGG	+	chr4.2:9638789-9638808	MS.gene58124:CDS	40.0%
	GGTAAAACCAGGACATCTTT+GGG	+	chr4.2:9638359-9638378	MS.gene58124:intron	40.0%
	GTCACAGATGTAGGCAAAAT+TGG	+	chr4.2:9639404-9639423	MS.gene58124:intron	40.0%
	TACACACTCTCAAGAACGAT+TGG	+	chr4.2:9636876-9636895	MS.gene58124:CDS	40.0%
	TAGGTTCGTGTCCCTTTATA+AGG	-	chr4.2:9638588-9638607	None:intergenic	40.0%
	TATAAAGGGACACGAACCTA+TGG	+	chr4.2:9638588-9638607	MS.gene58124:CDS	40.0%
	TCTGTCTTTCCATTAGTTGC+AGG	+	chr4.2:9639706-9639725	MS.gene58124:CDS	40.0%
	TGTGTCTATGATGTAGTCCA+TGG	-	chr4.2:9639590-9639609	None:intergenic	40.0%
!	ATTTTGTGGTTGTTCACCAG+CGG	+	chr4.2:9638622-9638641	MS.gene58124:CDS	40.0%
!	CAATTTTATGCAGGCTGCTA+TGG	+	chr4.2:9639561-9639580	MS.gene58124:intron	40.0%
!	CCCCCAGTTTTCAAAATTTC+TGG	+	chr4.2:9639293-9639312	MS.gene58124:intron	40.0%
!	CTGCAGTTTTCTATCTTTGG+AGG	+	chr4.2:9638209-9638228	MS.gene58124:intron	40.0%
!	CTTCATTTTCTCAGGGTCTT+TGG	-	chr4.2:9638910-9638929	None:intergenic	40.0%
!	CTTTTGTGGTTGTGTTTTGC+AGG	+	chr4.2:9638397-9638416	MS.gene58124:intron	40.0%
!	TGGTGGCTATTCTGATTTTG+TGG	+	chr4.2:9638608-9638627	MS.gene58124:CDS	40.0%
!!	AAAAGCTTTGAGTGGGAGTT+CGG	-	chr4.2:9636636-9636655	None:intergenic	40.0%
	AAATCAACACGGCCATGGAA+AGG	+	chr4.2:9640066-9640085	MS.gene58124:CDS	45.0%
	AACTCTGCAGTCACAGATGT+AGG	+	chr4.2:9639395-9639414	MS.gene58124:intron	45.0%
	AACTTCAGTAGACCAAGCAG+TGG	-	chr4.2:9639637-9639656	None:intergenic	45.0%
	AATGGGAAACTGGTCACTGT+AGG	+	chr4.2:9639658-9639677	MS.gene58124:CDS	45.0%
	ACTACTTACCCTGTTGTTCC+TGG	+	chr4.2:9636905-9636924	MS.gene58124:CDS	45.0%
	AGACATACCCAGGAACAACA+GGG	-	chr4.2:9636916-9636935	None:intergenic	45.0%
	ATGCATGTGTGATGTGGTGT+GGG	+	chr4.2:9639087-9639106	MS.gene58124:intron	45.0%
	ATGGAAAGACAGAGAGCTCA+AGG	-	chr4.2:9639699-9639718	None:intergenic	45.0%
	ATGGCCTCAGTTTCCTTGTT+TGG	-	chr4.2:9638855-9638874	None:intergenic	45.0%
	ATGGGAAACTGGTCACTGTA+GGG	+	chr4.2:9639659-9639678	MS.gene58124:CDS	45.0%
	CACACTCTCAAGAACGATTG+GGG	+	chr4.2:9636878-9636897	MS.gene58124:CDS	45.0%
	CTAATGTCTTGACTGCAGGA+CGG	+	chr4.2:9639934-9639953	MS.gene58124:intron	45.0%
	CTACTTACCCTGTTGTTCCT+GGG	+	chr4.2:9636906-9636925	MS.gene58124:CDS	45.0%
	CTCCCTTTCATTTCTCTCGA+AGG	+	chr4.2:9636690-9636709	MS.gene58124:CDS	45.0%
	CTCTCCAAACAAGGAAACTG+AGG	+	chr4.2:9638848-9638867	MS.gene58124:CDS	45.0%
	CTTATCGGAGGGAGTAACAT+TGG	+	chr4.2:9639960-9639979	MS.gene58124:CDS	45.0%
	GAAACTGAACTACATACCGC+TGG	-	chr4.2:9638641-9638660	None:intergenic	45.0%
	GACTGAACCAGGCAAACATT+TGG	+	chr4.2:9638743-9638762	MS.gene58124:CDS	45.0%
	GAGCATAACCTAGACGAAAC+AGG	-	chr4.2:9638965-9638984	None:intergenic	45.0%
	GTTGGCGTTGTGACAAAAGT+TGG	+	chr4.2:9638429-9638448	MS.gene58124:CDS	45.0%
	TATAAATGGGCAGGTCGCTA+GGG	+	chr4.2:9639060-9639079	MS.gene58124:intron	45.0%
	TGGAAAGACAGAGAGCTCAA+GGG	-	chr4.2:9639698-9639717	None:intergenic	45.0%
	TTATAAATGGGCAGGTCGCT+AGG	+	chr4.2:9639059-9639078	MS.gene58124:intron	45.0%
	TTCAAGTGCACTGCAAAGAC+TGG	+	chr4.2:9638113-9638132	MS.gene58124:intron	45.0%
	TTTGAGTGGGAGTTCGGTTT+CGG	-	chr4.2:9636630-9636649	None:intergenic	45.0%
!	AGCTTTGTGAAGCCTTTCCA+TGG	-	chr4.2:9640081-9640100	None:intergenic	45.0%
!	CTCAGTTTCCTTGTTTGGAG+AGG	-	chr4.2:9638850-9638869	None:intergenic	45.0%
!	GAACTCCCACTCAAAGCTTT+TGG	+	chr4.2:9636635-9636654	MS.gene58124:CDS	45.0%
!	TCCCACTCAAAGCTTTTGGT+TGG	+	chr4.2:9636639-9636658	MS.gene58124:CDS	45.0%
!	TCTCAGGGTCTTTGGAAACA+AGG	-	chr4.2:9638902-9638921	None:intergenic	45.0%
!	TCTGTTCGTAAGGGCTTGTT+TGG	-	chr4.2:9637604-9637623	None:intergenic	45.0%
!	TTCAGAGTGGGTGATAACGT+TGG	+	chr4.2:9638468-9638487	MS.gene58124:CDS	45.0%
!!	CCCAACCAAAAGCTTTGAGT+GGG	-	chr4.2:9636643-9636662	None:intergenic	45.0%
!!	CCCACTCAAAGCTTTTGGTT+GGG	+	chr4.2:9636640-9636659	MS.gene58124:CDS	45.0%
!!!	TTTTATTTTTATTTTTTGTA+GGG	+	chr4.2:9636793-9636812	MS.gene58124:intron	5.0%
!!!	TTTTTATTTTTATTTTTTGT+AGG	+	chr4.2:9636792-9636811	MS.gene58124:intron	5.0%
	AAAGGGACACGAACCTATGG+TGG	+	chr4.2:9638591-9638610	MS.gene58124:CDS	50.0%
	ACCAGGCAAACATTTGGGAG+TGG	+	chr4.2:9638749-9638768	MS.gene58124:CDS	50.0%
	ACTGCAGGACGGAAGCTTAT+CGG	+	chr4.2:9639945-9639964	MS.gene58124:intron	50.0%
	ATCGGAGGGAGTAACATTGG+AGG	+	chr4.2:9639963-9639982	MS.gene58124:CDS	50.0%
	CAGACATACCCAGGAACAAC+AGG	-	chr4.2:9636917-9636936	None:intergenic	50.0%
	CAGGAGATGCTCGATTTCTG+CGG	+	chr4.2:9639999-9640018	MS.gene58124:CDS	50.0%
	GATGCATGTGTGATGTGGTG+TGG	+	chr4.2:9639086-9639105	MS.gene58124:intron	50.0%
	GCAAACATTTGGGAGTGGCA+GGG	+	chr4.2:9638754-9638773	MS.gene58124:CDS	50.0%
	GCATGAAATCAACACGGCCA+TGG	+	chr4.2:9640061-9640080	MS.gene58124:CDS	50.0%
	GGAAAGACAGAGAGCTCAAG+GGG	-	chr4.2:9639697-9639716	None:intergenic	50.0%
	TAGACCAAGCAGTGGCATCA+GGG	-	chr4.2:9639629-9639648	None:intergenic	50.0%
	TATGAAGCACGGACACTCCT+CGG	-	chr4.2:9637880-9637899	None:intergenic	50.0%
	TCGGAGGGAGTAACATTGGA+GGG	+	chr4.2:9639964-9639983	MS.gene58124:CDS	50.0%
	TGGAGGGATGAAAGAGACTC+AGG	+	chr4.2:9639980-9639999	MS.gene58124:CDS	50.0%
	TTGAGTGGGAGTTCGGTTTC+GGG	-	chr4.2:9636629-9636648	None:intergenic	50.0%
!	CGGTAAAGCATTTGGGCTGA+AGG	+	chr4.2:9638809-9638828	MS.gene58124:CDS	50.0%
!	GTGGGTGATAACGTTGGTGT+TGG	+	chr4.2:9638474-9638493	MS.gene58124:CDS	50.0%
!!	GCCCAACCAAAAGCTTTGAG+TGG	-	chr4.2:9636644-9636663	None:intergenic	50.0%
!!	TACAAGTGTCGGTCTCGTGT+TGG	+	chr4.2:9637818-9637837	MS.gene58124:intron	50.0%
	CAGGACGGAAGCTTATCGGA+GGG	+	chr4.2:9639949-9639968	MS.gene58124:intron	55.0%
	CATTCCCTGATGCCACTGCT+TGG	+	chr4.2:9639622-9639641	MS.gene58124:CDS	55.0%
	GACAACTTACCCCTTGACGC+TGG	+	chr4.2:9638663-9638682	MS.gene58124:CDS	55.0%
	GATTAGGCGTGTCCTGGTGT+CGG	-	chr4.2:9637858-9637877	None:intergenic	55.0%
	GCCACTCCCAAATGTTTGCC+TGG	-	chr4.2:9638753-9638772	None:intergenic	55.0%
	GCGTCAAGGGGTAAGTTGTC+AGG	-	chr4.2:9638663-9638682	None:intergenic	55.0%
	GGACGGATGCATGTGTGATG+TGG	+	chr4.2:9639081-9639100	MS.gene58124:intron	55.0%
	GGCAAACATTTGGGAGTGGC+AGG	+	chr4.2:9638753-9638772	MS.gene58124:CDS	55.0%
	GTAGACCAAGCAGTGGCATC+AGG	-	chr4.2:9639630-9639649	None:intergenic	55.0%
	TGAAGCACGGATACGGACAC+CGG	+	chr4.2:9637740-9637759	MS.gene58124:intron	55.0%
	TGGGCTGCTAGAGACACTTC+TGG	+	chr4.2:9636659-9636678	MS.gene58124:CDS	55.0%
!	AAGTGGAGCACCAGCGTCAA+GGG	-	chr4.2:9638676-9638695	None:intergenic	55.0%
!	TGTCGGTCTCGTGTTGGTGT+CGG	+	chr4.2:9637824-9637843	MS.gene58124:intron	55.0%
	AATGGGCAGGTCGCTAGGGA+CGG	+	chr4.2:9639064-9639083	MS.gene58124:intron	60.0%
	ACCAGGACACGCCTAATCCG+AGG	+	chr4.2:9637860-9637879	MS.gene58124:intron	60.0%
	CATTTGGGAGTGGCAGGGCT+TGG	+	chr4.2:9638759-9638778	MS.gene58124:CDS	60.0%
	CGGGCTATACACGGTGATCC+CGG	-	chr4.2:9638706-9638725	None:intergenic	60.0%
	GCACGGACACTCCTCGGATT+AGG	-	chr4.2:9637874-9637893	None:intergenic	60.0%
	GCAGGACGGAAGCTTATCGG+AGG	+	chr4.2:9639948-9639967	MS.gene58124:intron	60.0%
	GCAGGCTGCTATGGGAACCA+TGG	+	chr4.2:9639570-9639589	MS.gene58124:intron	60.0%
	GGTGCTCCACTTCTGTGTGC+CGG	+	chr4.2:9638684-9638703	MS.gene58124:CDS	60.0%
	GTGATCCCGGCACACAGAAG+TGG	-	chr4.2:9638693-9638712	None:intergenic	60.0%
	GTGCTCCACTTCTGTGTGCC+GGG	+	chr4.2:9638685-9638704	MS.gene58124:CDS	60.0%
	TCGGACATGTGTCCGACACC+AGG	+	chr4.2:9637843-9637862	MS.gene58124:intron	60.0%
!	AGTGGAGCACCAGCGTCAAG+GGG	-	chr4.2:9638675-9638694	None:intergenic	60.0%
!	GAAGTGGAGCACCAGCGTCA+AGG	-	chr4.2:9638677-9638696	None:intergenic	60.0%
!	GAGAGCTCAAGGGGCTTGCT+AGG	-	chr4.2:9639688-9639707	None:intergenic	60.0%
!	TCCTCGGATTAGGCGTGTCC+TGG	-	chr4.2:9637864-9637883	None:intergenic	60.0%
!	TGTGTCGGCGTGTCAGTGTC+CGG	-	chr4.2:9637762-9637781	None:intergenic	60.0%
	TTGGGAGTGGCAGGGCTTGG+TGG	+	chr4.2:9638762-9638781	MS.gene58124:CDS	65.0%
!	TGGGAGTGGCAGGGCTTGGT+GGG	+	chr4.2:9638763-9638782	MS.gene58124:CDS	65.0%
!!	GTGGCAGGGCTTGGTGGGTT+AGG	+	chr4.2:9638768-9638787	MS.gene58124:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	9636614	9640154	9636614	ID=MS.gene58124
chr4.2	mRNA	9636614	9640154	9636614	ID=MS.gene58124.t1;Parent=MS.gene58124
chr4.2	exon	9636614	9636753	9636614	ID=MS.gene58124.t1.exon1;Parent=MS.gene58124.t1
chr4.2	CDS	9636614	9636753	9636614	ID=cds.MS.gene58124.t1;Parent=MS.gene58124.t1
chr4.2	exon	9636814	9636927	9636814	ID=MS.gene58124.t1.exon2;Parent=MS.gene58124.t1
chr4.2	CDS	9636814	9636927	9636814	ID=cds.MS.gene58124.t1;Parent=MS.gene58124.t1
chr4.2	exon	9638419	9638929	9638419	ID=MS.gene58124.t1.exon3;Parent=MS.gene58124.t1
chr4.2	CDS	9638419	9638929	9638419	ID=cds.MS.gene58124.t1;Parent=MS.gene58124.t1
chr4.2	exon	9639574	9639727	9639574	ID=MS.gene58124.t1.exon4;Parent=MS.gene58124.t1
chr4.2	CDS	9639574	9639727	9639574	ID=cds.MS.gene58124.t1;Parent=MS.gene58124.t1
chr4.2	exon	9639952	9640154	9639952	ID=MS.gene58124.t1.exon5;Parent=MS.gene58124.t1
chr4.2	CDS	9639952	9640154	9639952	ID=cds.MS.gene58124.t1;Parent=MS.gene58124.t1

Gene Sequence

>MS.gene58124

ATGGCGAAATCACCCGAAACCGAACTCCCACTCAAAGCTTTTGGTTGGGCTGCTAGAGACACTTCTGGCACACTCTCTCCCTTTCATTTCTCTCGAAGGTTCTTTTCTTTTCTTTTTTTTTTCTTTTTCTTTTCATCTCAGTCTTTATAATTTTTTACGTGATTAATTTTTATTTTTATTTTTATTTTTATTTTTTGTAGGGAAAATGGTGATGATGATGTAAGTGTGAAAATTCTATATTGTGGAGTTTGTCATTCTGATCTACACACTCTCAAGAACGATTGGGGTTTCACTACTTACCCTGTTGTTCCTGGGTATGTCTGTTTTTCTTTTCTCTCTTTCTTTTACATTTTGCAGTGCATAATAGTATCATGCAAATCTCACTCTCTGATAGTATTTTTAATTATTATTATTATTATATTATTATATTCGACTTTGTTTATTGCCGTCTTTCTGTTGAATTGAATAGAAAAATGGATACAATAGTTGAATCCCAGTTCTTTACCCCTCAAATTAAAATTAGAGGTGGAGGTTGTTTTTAAACTTAAAATATAATATATGATCTCACATCATGAATTATATATTGTTACTTTTTTCACTAAAAGACGCTCTGCCTGCTTGATGATTATGTCATACTCATACATACTTGTCTATTAGAATTTGGATCTAAATTCTTAATTCACTTTATTTACCTTTGGAATTATGTGTATGTTTGGATTGTTTTATTTGAGTTTATCTATTGACATAAGTTTTGTGAGATTGTTTGAAAGAACTTGTGAAAACACCTTATGAGAATTTTCATAAGCTTTTTTTAACTTATTTTCGTAAGTTCTTTGATATAGCTTATAAAAACAACTTATATCATATACAAAAACAATTTAACATTATTTTTTCTCTAGCTATAAAAATAGCTTATGCAAGCTTATACATAAGCGCTTATCATGATAAGTAAGTGCATGTACTTCAAGCTTCAAATAAGTTGTTTATCCAAACAAGCCCTTACGAACAGATAATTAAATAAACCTATTATATGAATAACCACCTACCTGTTTTTAATCATGTTTAACACACTTAGGCACTACATCACCTTAATTTGAATCCTCAAAAGTGAATAGTTGCAAAGCTATGAAGCACGGATACGGACACCGGACACTGACACGCCGACACACTAATAATTTGAAAAAATCACATAATTAAGTGTAATTACAAGTGTCGGTCTCGTGTTGGTGTCGGACATGTGTCCGACACCAGGACACGCCTAATCCGAGGAGTGTCCGTGCTTCATAGTTGCAAAGTATATCTATCATTGATTAATAAGTTAATCAATGAGCGTGTTGAAATATCAAATTTTTCATTCTTTTTCATTAATGGTTTATGGTACTTAAGTTTCTTCTTCATTAATCCTAGTAAACTATTGGCCCATGTAACCTCAAAATTACATAATGTGTCTGTGTTACAAATGAAGTCTAGGTGTGTTACAATTTTACAACTAATAATTATTCAAGTGCACTGCAAAGACTGGAAATAAGGATGATATGTATGCGATTTTTGGTGGAAATGATGCAATATTCCCGATCTAGAAGCGGAAACTTTCACTGCAGTTTTCTATCTTTGGAGGAAATGATGCAATATTTGATTGTTAAATTAGTTGGTTTCTCATCCTTGACTATTGATTGATAGTTTATGCGTGCATGGCTTTGTGCAATAGTCAATATTGGACATTACTTGCTTATAAGTTATATGAAGGTAAAACCAGGACATCTTTGGGATTAACTTGCATTGACTTTTGTGGTTGTGTTTTGCAGGCATGAAATTGTTGGCGTTGTGACAAAAGTTGGAATCAATGTGAAAAAATTCAGAGTGGGTGATAACGTTGGTGTTGGTGTAATTGTCGAGTCTTGTCAGACTTGTGAGAATTGCAATCAAGATTTGGAGCAGTACTGTCCTAAACCTGTATTTACCTATAACTCTCCTTATAAAGGGACACGAACCTATGGTGGCTATTCTGATTTTGTGGTTGTTCACCAGCGGTATGTAGTTCAGTTTCCTGACAACTTACCCCTTGACGCTGGTGCTCCACTTCTGTGTGCCGGGATCACCGTGTATAGCCCGATGAAGTATTATGGTATGACTGAACCAGGCAAACATTTGGGAGTGGCAGGGCTTGGTGGGTTAGGCCATGTTGCAATCAAATTCGGTAAAGCATTTGGGCTGAAGGTTACTGTTATTAGTACCTCTCCAAACAAGGAAACTGAGGCCATTGATAAACTTGGTGCTGATTCTTTCCTTGTTTCCAAAGACCCTGAGAAAATGAAGGTAAGATACCACACCATTAACTTTAAAACCAACCTGTTTCGTCTAGGTTATGCTCTCCGCTGTTTTACTCTTATTTCGTCATACAGTTCTATATAGGAGTTGTTATGGATAATGATATCCATAGTTTACTTATAAATGGGCAGGTCGCTAGGGACGGATGCATGTGTGATGTGGTGTGGGCTTGTGCCTACACAAAATATTTTAAATTTTTATTTTTAAGTAGAATAATATAGTAATTGTTGGCATTTAGACCAAATAACCTACACTTATTCTCTCTTTGCCCACACTTAAATCACACTTGACACAACAACACAACCTTATTTCTGAATATATATTTAATGCTTCCAATTAATATATATAGTGCCCCCAGTTTTCAAAATTTCTGGATCCGTCAATGAGTATATAAACAAAATTAAATCTTGTAAACCTGAGTTTGAATAATAGAAAGGCACTGCAGTTTGCAGCAACTCTGCAGTCACAGATGTAGGCAAAATTGGTTGAACTCCATAATCAAATCATGTGTTCTCTGTTTGCGTTTTTCATATTTGTTTTCTTTTGGACCAGGTTGTTGTAACAAAAACAATGGATTGATTGGTTTAATATCTGCTTATAATGCATTTGTGTTTAAATACAATTTTATGCAGGCTGCTATGGGAACCATGGACTACATCATAGACACAATATCTGCTGCTCATTCCCTGATGCCACTGCTTGGTCTACTGAAGTTGAATGGGAAACTGGTCACTGTAGGGTTGCCTAGCAAGCCCCTTGAGCTCTCTGTCTTTCCATTAGTTGCAGGTAAACCATCAATAATTCTCTGTCAATTCTTTAATGCAATAATATGTGAATTTGAATTGATGTTTGAAACCAAGAATGAGTCTGTTACTTCCAAATCTGACCTGAAACATTTATACATGCTCATCTATTCTTGAAACTTTCAACTACTCTACATTTCATTATGATACAGAGATTTTGACCAATGAATTATCCAAATTTTCACATGTCTAATGTCTTGACTGCAGGACGGAAGCTTATCGGAGGGAGTAACATTGGAGGGATGAAAGAGACTCAGGAGATGCTCGATTTCTGCGGCAAGCACAACATAACTGCAGATATTGAGTTGATTAAGATGCATGAAATCAACACGGCCATGGAAAGGCTTCACAAAGCTGATGTGAAATATCGGTTTGTGATTGATGTGGCCAACTCCTTCTCAAGTTTGTAG

Protein sequence

>MS.gene58124.t1

MAKSPETELPLKAFGWAARDTSGTLSPFHFSRRFFSFLFFFFFFSSQENGDDDVSVKILYCGVCHSDLHTLKNDWGFTTYPVVPGHEIVGVVTKVGINVKKFRVGDNVGVGVIVESCQTCENCNQDLEQYCPKPVFTYNSPYKGTRTYGGYSDFVVVHQRYVVQFPDNLPLDAGAPLLCAGITVYSPMKYYGMTEPGKHLGVAGLGGLGHVAIKFGKAFGLKVTVISTSPNKETEAIDKLGADSFLVSKDPEKMKAAMGTMDYIIDTISAAHSLMPLLGLLKLNGKLVTVGLPSKPLELSVFPLVAGRKLIGGSNIGGMKETQEMLDFCGKHNITADIELIKMHEINTAMERLHKADVKYRFVIDVANSFSSL