AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene069146

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069146.t1 MTR_5g031310 95.856 362 15 0 1 362 1 362 0.0 694
MS.gene069146.t1 MTR_5g031305 93.370 362 24 0 1 362 1 362 0.0 682
MS.gene069146.t1 MTR_5g031300 84.211 361 57 0 1 361 1 361 0.0 625
MS.gene069146.t1 MTR_5g031360 86.592 358 48 0 5 362 6 363 0.0 624
MS.gene069146.t1 MTR_5g031230 82.548 361 62 1 1 361 1 360 0.0 597
MS.gene069146.t1 MTR_4g115300 82.203 354 63 0 8 361 7 360 0.0 591
MS.gene069146.t1 MTR_5g031390 82.486 354 62 0 8 361 7 360 0.0 587
MS.gene069146.t1 MTR_5g031250 80.055 361 71 1 1 361 1 360 0.0 576
MS.gene069146.t1 MTR_5g031210 79.379 354 73 0 8 361 7 360 0.0 562
MS.gene069146.t1 MTR_5g031190 75.637 353 86 0 9 361 8 360 0.0 549
MS.gene069146.t1 MTR_5g031180 79.944 354 71 0 8 361 7 360 0.0 546
MS.gene069146.t1 MTR_8g068190 78.814 354 75 0 8 361 7 360 0.0 540
MS.gene069146.t1 MTR_8g092630 61.050 362 135 2 2 358 53 413 2.27e-149 428
MS.gene069146.t1 MTR_5g031430 59.710 345 139 0 15 359 14 358 6.35e-143 409
MS.gene069146.t1 MTR_5g031250 81.405 242 44 1 1 242 1 241 2.97e-138 394
MS.gene069146.t1 MTR_1g025950 56.897 348 149 1 10 356 8 355 1.08e-131 381
MS.gene069146.t1 MTR_1g107425 51.020 343 168 0 12 354 9 351 3.02e-121 354
MS.gene069146.t1 MTR_2g009810 53.602 347 157 3 15 359 62 406 7.95e-117 345
MS.gene069146.t1 MTR_1g107425 51.211 289 140 1 12 299 9 297 3.34e-96 288
MS.gene069146.t1 MTR_2g009820 52.155 232 108 2 131 359 23 254 5.49e-64 204
MS.gene069146.t1 MTR_5g031290 76.522 115 27 0 248 362 80 194 6.96e-55 179
MS.gene069146.t1 MTR_5g031290 82.716 81 12 2 1 80 1 80 7.19e-41 142
MS.gene069146.t1 MTR_8g095220 26.599 297 186 8 11 279 51 343 1.95e-21 95.1
MS.gene069146.t1 MTR_8g095220 28.125 256 157 7 11 243 51 302 3.59e-21 93.2
MS.gene069146.t1 MTR_0001s0660 28.852 305 187 12 39 319 35 333 6.60e-21 94.0
MS.gene069146.t1 MTR_6g465010 27.830 212 139 5 26 234 13 213 1.77e-16 80.1
MS.gene069146.t1 MTR_6g466320 28.638 213 136 6 26 234 29 229 2.40e-16 79.7
MS.gene069146.t1 MTR_6g465010 28.638 213 136 6 26 234 29 229 2.40e-16 79.7
MS.gene069146.t1 MTR_3g089977 24.339 378 242 13 12 355 12 379 1.58e-15 77.4
MS.gene069146.t1 MTR_3g089940 24.728 368 234 12 12 345 12 370 2.69e-15 76.6
MS.gene069146.t1 MTR_3g089940 24.274 379 244 12 1 345 1 370 5.39e-15 75.9
MS.gene069146.t1 MTR_5g045710 24.405 336 221 11 39 345 35 366 1.92e-14 74.3
MS.gene069146.t1 MTR_3g089940 25.856 263 168 7 12 254 12 267 2.77e-14 73.6
MS.gene069146.t1 MTR_3g089940 25.182 274 178 7 1 254 1 267 4.80e-14 72.8
MS.gene069146.t1 MTR_3g089970 24.317 366 234 12 14 345 15 371 6.99e-14 72.4
MS.gene069146.t1 MTR_7g068320 26.377 345 200 13 40 341 48 381 9.21e-14 72.4
MS.gene069146.t1 MTR_7g068330 26.087 345 200 14 40 341 41 373 1.66e-12 68.6
MS.gene069146.t1 MTR_1g069430 26.471 340 214 13 1 313 1 331 4.35e-12 67.0
MS.gene069146.t1 MTR_7g099040 24.930 357 225 13 2 328 4 347 1.36e-11 65.5
MS.gene069146.t1 MTR_5g031380 64.103 39 14 0 81 119 19 57 2.25e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069146.t1 AT4G37980 71.709 357 99 1 6 362 3 357 1.61e-175 492
MS.gene069146.t1 AT4G37990 70.868 357 102 1 6 362 3 357 1.83e-171 482
MS.gene069146.t1 AT4G37970 63.173 353 130 0 9 361 9 361 1.91e-163 461
MS.gene069146.t1 AT4G39330 62.500 352 132 0 9 360 8 359 2.03e-162 459
MS.gene069146.t1 AT2G21730 60.571 350 136 2 13 360 6 355 4.68e-150 428
MS.gene069146.t1 AT2G21890 59.885 349 139 1 13 360 6 354 6.07e-150 428
MS.gene069146.t1 AT4G37980 70.470 298 86 1 6 303 3 298 3.92e-135 387
MS.gene069146.t1 AT4G39330 62.162 296 112 0 9 304 8 303 3.06e-131 378
MS.gene069146.t1 AT4G37970 61.724 290 111 0 9 298 9 298 8.84e-127 366
MS.gene069146.t1 AT3G19450 52.312 346 165 0 12 357 9 354 5.69e-115 338
MS.gene069146.t1 AT1G72680 50.437 343 169 1 17 359 14 355 1.36e-113 335
MS.gene069146.t1 AT4G34230 50.575 348 172 0 7 354 3 350 1.01e-106 317
MS.gene069146.t1 AT4G34230 50.287 348 173 0 7 354 3 350 9.29e-106 315
MS.gene069146.t1 AT4G37970 60.924 238 93 0 9 246 9 246 5.98e-101 299
MS.gene069146.t1 AT4G37970 61.277 235 91 0 9 243 9 243 4.53e-100 296
MS.gene069146.t1 AT5G63620 26.207 290 186 6 16 279 58 345 4.09e-23 100
MS.gene069146.t1 AT5G63620 26.207 290 186 6 16 279 58 345 5.95e-23 99.8
MS.gene069146.t1 AT5G63620 27.711 249 157 5 16 243 58 304 2.60e-22 96.3
MS.gene069146.t1 AT1G22430 26.183 317 202 12 40 328 42 354 3.47e-17 82.4
MS.gene069146.t1 AT1G22430 26.183 317 202 12 40 328 42 354 3.47e-17 82.4
MS.gene069146.t1 AT5G43940 26.648 349 216 12 1 320 1 338 2.46e-16 80.1
MS.gene069146.t1 AT5G43940 26.774 310 191 9 40 320 48 350 7.29e-16 78.6
MS.gene069146.t1 AT5G51970 25.490 255 169 6 37 279 40 285 8.24e-16 78.2
MS.gene069146.t1 AT5G51970 25.490 255 169 6 37 279 40 285 8.24e-16 78.2
MS.gene069146.t1 AT1G32780 26.316 361 221 13 40 359 37 393 4.35e-15 76.3
MS.gene069146.t1 AT4G22110 25.373 335 214 13 40 344 43 371 3.55e-14 73.6
MS.gene069146.t1 AT4G22110 25.373 335 214 13 40 344 43 371 3.55e-14 73.6
MS.gene069146.t1 AT1G22430 26.000 200 129 5 40 222 42 239 4.39e-13 69.7
MS.gene069146.t1 AT1G22430 26.000 200 129 5 40 222 42 239 6.35e-13 68.9
MS.gene069146.t1 AT1G22430 26.000 200 129 5 40 222 42 239 6.35e-13 68.9
MS.gene069146.t1 AT1G77120 22.597 385 261 13 1 355 1 378 2.56e-12 67.8
MS.gene069146.t1 AT5G42250 21.408 341 235 10 40 353 45 379 1.51e-11 65.5

Find 92 sgRNAs with CRISPR-Local

Find 113 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAGTAGCTGGAAGTTTAAT+TGG 0.186001 5.3:-20354265 MS.gene069146:CDS
TATAGTCCTCTTCGACATTT+TGG 0.197168 5.3:-20354849 MS.gene069146:CDS
AAACCAATCAATGGTAAAAT+AGG 0.229602 5.3:+20354493 None:intergenic
TATCGATTCGTGATTGATAT+TGG 0.245698 5.3:-20354115 MS.gene069146:CDS
GTGAAATTCGCCAAAGCTTT+TGG 0.268679 5.3:-20354765 MS.gene069146:CDS
ATTGGTTTATTGAAATCTAA+TGG 0.271827 5.3:-20354478 MS.gene069146:CDS
CCCTGGAACTAATGGATATA+TGG 0.285088 5.3:+20355332 None:intergenic
TCCTGCAATGTTTAAGGGCT+TGG 0.287864 5.3:+20354079 None:intergenic
TCTTGGTGGACTTGGTCATA+TGG 0.309264 5.3:-20354790 MS.gene069146:CDS
ACCACATGTGACTGTTTGTT+GGG 0.314826 5.3:+20355010 None:intergenic
CCCATTCATTCTTCATCATA+TGG 0.324277 5.3:+20355361 None:intergenic
TAAGTCAACTTTAGGGAAAC+TGG 0.329890 5.3:-20355438 MS.gene069146:intron
GCCCAACCAAAGGCCTTCTT+AGG 0.362894 5.3:+20355686 None:intergenic
CACCACATGTGACTGTTTGT+TGG 0.363970 5.3:+20355009 None:intergenic
AAGTTTAATTGGAGGGATAA+AGG 0.373525 5.3:-20354254 MS.gene069146:CDS
GTTGTTGGTCTTGGTGGACT+TGG 0.377317 5.3:-20354798 MS.gene069146:CDS
GTTACGAGATGTGCTAATTA+CGG 0.380792 5.3:+20354731 None:intergenic
ATAGGAATAACCTCAATATC+AGG 0.384692 5.3:+20354187 None:intergenic
TCCATATATCCATTAGTTCC+AGG 0.385414 5.3:-20355333 MS.gene069146:CDS
AACTTACCTCTTGATGTTGC+CGG 0.388597 5.3:-20354900 MS.gene069146:CDS
TCCTAAGAAGGCCTTTGGTT+GGG 0.390941 5.3:-20355687 MS.gene069146:CDS
ACATCAAGAGGTAAGTTATC+AGG 0.391560 5.3:+20354906 None:intergenic
CGTGATGGCACCGTCACATA+TGG 0.395744 5.3:-20354978 MS.gene069146:CDS
GAGCATCCTAAGAAGGCCTT+TGG 0.400649 5.3:-20355692 MS.gene069146:CDS
ATAGGTGTTGTTGGTCTTGG+TGG 0.407624 5.3:-20354804 MS.gene069146:CDS
GCCGGTCCTCTTCTTTGCGC+TGG 0.408424 5.3:-20354882 MS.gene069146:CDS
CACATTAGCACCAAAAGCTT+TGG 0.409643 5.3:+20354755 None:intergenic
CCCCTTTCAATTTCTCCAGA+AGG 0.415467 5.3:-20355637 MS.gene069146:intron
AATATAGGTGTTGTTGGTCT+TGG 0.416186 5.3:-20354807 MS.gene069146:CDS
TCAAATACCTGCATCTGATC+TGG 0.416400 5.3:+20354651 None:intergenic
AAATGCAGGCATGAACTTGC+GGG 0.418681 5.3:-20355158 MS.gene069146:intron
GAAGCATACCCTGGAACTAA+TGG 0.426302 5.3:+20355324 None:intergenic
AAAGTGGAAAAGTTTAAAGT+TGG 0.429905 5.3:-20355113 MS.gene069146:CDS
GTAGGGAGAAAGTTAGTAGC+TGG 0.438913 5.3:-20354277 MS.gene069146:intron
CATTCAAGGTGTTGTATTGT+GGG 0.442854 5.3:-20355401 MS.gene069146:CDS
TTTAAAGTTGGAGACAAAGT+AGG 0.443860 5.3:-20355101 MS.gene069146:CDS
TCCATATGATGAAGAATGAA+TGG 0.444527 5.3:-20355362 MS.gene069146:CDS
GACGGTGCCATCACGATACT+TGG 0.453032 5.3:+20354986 None:intergenic
TGGGCAGCTAGAGATCCTTC+TGG 0.459135 5.3:-20355668 MS.gene069146:CDS
TGAGAAAGATGTAGCATTCA+AGG 0.467351 5.3:-20355415 MS.gene069146:CDS
GACAAACCTGGTATGAATAT+AGG 0.467866 5.3:-20354822 MS.gene069146:CDS
GCTGTGTTGACATAATCAAT+AGG 0.471251 5.3:+20354169 None:intergenic
CAAAGTAGGTGTGGGATACA+TGG 0.478011 5.3:-20355087 MS.gene069146:CDS
CACATCTCGTAACAAAGAAA+AGG 0.483022 5.3:-20354721 MS.gene069146:CDS
TTCCACTTTGCTTCCTACCT+CGG 0.483575 5.3:+20355127 None:intergenic
CCATATGATGAAGAATGAAT+GGG 0.485124 5.3:-20355361 MS.gene069146:CDS
CCTCCAAGTAAAGAAAATAC+AGG 0.487127 5.3:+20354412 None:intergenic
GATTTCAATAAACCAATCAA+TGG 0.487235 5.3:+20354484 None:intergenic
CAATGTAAAGCCTGATATTG+AGG 0.489359 5.3:-20354197 MS.gene069146:CDS
TGGAGGTTATTCTGACTCAA+TGG 0.494148 5.3:-20354958 MS.gene069146:CDS
ACCTTCTGGAGAAATTGAAA+GGG 0.500807 5.3:+20355636 None:intergenic
ATCCTAAGAAGGCCTTTGGT+TGG 0.502470 5.3:-20355688 MS.gene069146:CDS
AACCTTCTGGAGAAATTGAA+AGG 0.504412 5.3:+20355635 None:intergenic
GGTATGAATATAGGTGTTGT+TGG 0.507632 5.3:-20354813 MS.gene069146:CDS
AAAGGAAGCAATAGAACACT+TGG 0.509890 5.3:-20354703 MS.gene069146:CDS
GCATTCAAGGTGTTGTATTG+TGG 0.522756 5.3:-20355402 MS.gene069146:CDS
AGTGTTAGAGCATCCTAAGA+AGG 0.523098 5.3:-20355699 MS.gene069146:CDS
GCTGCAACAGGTACTTTGAA+TGG 0.526500 5.3:-20354544 MS.gene069146:CDS
TAGCTGGAAGTTTAATTGGA+GGG 0.528518 5.3:-20354261 MS.gene069146:CDS
AGTTGGAGACAAAGTAGGTG+TGG 0.528716 5.3:-20355096 MS.gene069146:CDS
CCACATGTGACTGTTTGTTG+GGG 0.540150 5.3:+20355011 None:intergenic
GAAAATACAGGAAGCTCTAG+AGG 0.546279 5.3:+20354424 None:intergenic
CCATATATCCATTAGTTCCA+GGG 0.551121 5.3:-20355332 MS.gene069146:intron
GTAGCTGGAAGTTTAATTGG+AGG 0.559892 5.3:-20354262 MS.gene069146:CDS
GGAAAGCTTGTAATGGTTGG+TGG 0.563386 5.3:-20354457 MS.gene069146:CDS
TCCAGCGCAAAGAAGAGGAC+CGG 0.564917 5.3:+20354881 None:intergenic
GTGACTCCAGCGCAAAGAAG+AGG 0.566494 5.3:+20354876 None:intergenic
TAAATGCAGGCATGAACTTG+CGG 0.570766 5.3:-20355159 MS.gene069146:intron
ATCTCTAGCTGCCCAACCAA+AGG 0.576625 5.3:+20355676 None:intergenic
GTTGGAGACAAAGTAGGTGT+GGG 0.583586 5.3:-20355095 MS.gene069146:CDS
ATCTAATGGAAAGCTTGTAA+TGG 0.587781 5.3:-20354464 MS.gene069146:CDS
CTGCGATCCAGATCAGATGC+AGG 0.590600 5.3:-20354658 MS.gene069146:intron
GCTTGTAATGGTTGGTGGAG+TGG 0.601696 5.3:-20354452 MS.gene069146:CDS
AGAGGACCGGCAACATCAAG+AGG 0.610980 5.3:+20354894 None:intergenic
AATGGAAAGCTTGTAATGGT+TGG 0.618001 5.3:-20354460 MS.gene069146:CDS
ATTCATTCTTCATCATATGG+AGG 0.623043 5.3:+20355364 None:intergenic
ACAACACCTATATTCATACC+AGG 0.624332 5.3:+20354816 None:intergenic
TTTGAATGGTATTATTGACA+CGG 0.632246 5.3:-20354530 MS.gene069146:CDS
AAAGGGGAGAGAACACCAGA+AGG 0.635132 5.3:+20355653 None:intergenic
GCGGGTATAGTAACCGAGGT+AGG 0.635211 5.3:-20355140 MS.gene069146:CDS
TGATTATGTCAACACAGCAG+TGG 0.636651 5.3:-20354164 MS.gene069146:CDS
TCGAGTCCAAAATGTCGAAG+AGG 0.641706 5.3:+20354843 None:intergenic
GATGGCACCGTCACATATGG+AGG 0.644175 5.3:-20354975 MS.gene069146:CDS
CCTTCTGGAGAAATTGAAAG+GGG 0.647504 5.3:+20355637 None:intergenic
AGAATAACCTCCATATGTGA+CGG 0.659885 5.3:+20354968 None:intergenic
CACATGTGACTGTTTGTTGG+GGG 0.661508 5.3:+20355012 None:intergenic
AACCGAGGTAGGAAGCAAAG+TGG 0.665272 5.3:-20355129 MS.gene069146:CDS
AAGGAAGCAATAGAACACTT+GGG 0.667331 5.3:-20354702 MS.gene069146:CDS
CCCCAACAAACAGTCACATG+TGG 0.678792 5.3:-20355011 MS.gene069146:CDS
CATATGATGAAGAATGAATG+GGG 0.695552 5.3:-20355360 MS.gene069146:CDS
TGTGGTGCCAAGTATCGTGA+TGG 0.704207 5.3:-20354993 MS.gene069146:CDS
ACTTGCGGGTATAGTAACCG+AGG 0.756724 5.3:-20355144 MS.gene069146:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGTAAGTATAAAGATATATA+TGG + chr5.3:20354553-20354572 None:intergenic 15.0%
!! ATAAATTTCACATTATTTGT+AGG - chr5.3:20355511-20355530 MS.gene069146:intron 15.0%
!! TAAATTTCACATTATTTGTA+GGG - chr5.3:20355512-20355531 MS.gene069146:intron 15.0%
!! TAAAGTATAATTGTAGTAGA+AGG + chr5.3:20354513-20354532 None:intergenic 20.0%
!! TACTAATTAAGTCAACTTTA+GGG - chr5.3:20354361-20354380 MS.gene069146:intron 20.0%
!! TGCTTATAATTAATAAATGC+AGG - chr5.3:20354634-20354653 MS.gene069146:intron 20.0%
!!! ATAATGTACCACATTTTAAT+AGG - chr5.3:20355240-20355259 MS.gene069146:intron 20.0%
!!! ATCAACTCATCTTTATTTAA+TGG - chr5.3:20354206-20354225 MS.gene069146:CDS 20.0%
!!! ATTGGTTTATTGAAATCTAA+TGG - chr5.3:20355328-20355347 MS.gene069146:intron 20.0%
! AAACCAATCAATGGTAAAAT+AGG + chr5.3:20355316-20355335 None:intergenic 25.0%
! CTACTAATTAAGTCAACTTT+AGG - chr5.3:20354360-20354379 MS.gene069146:intron 25.0%
! GATTTCAATAAACCAATCAA+TGG + chr5.3:20355325-20355344 None:intergenic 25.0%
! TTATAATGAAGTAAACCTTC+TGG + chr5.3:20354187-20354206 None:intergenic 25.0%
! TTTGAATGGTATTATTGACA+CGG - chr5.3:20355276-20355295 MS.gene069146:intron 25.0%
!! AAAGTGGAAAAGTTTAAAGT+TGG - chr5.3:20354693-20354712 MS.gene069146:CDS 25.0%
!! CTAAAGTTGACTTAATTAGT+AGG + chr5.3:20354362-20354381 None:intergenic 25.0%
!!! TCAAGAAATGATTGATTTTG+CGG - chr5.3:20355579-20355598 MS.gene069146:intron 25.0%
AAGTTTAATTGGAGGGATAA+AGG - chr5.3:20355552-20355571 MS.gene069146:intron 30.0%
ATAATTGTAGTAGAAGGAGT+TGG + chr5.3:20354507-20354526 None:intergenic 30.0%
ATAGGAATAACCTCAATATC+AGG + chr5.3:20355622-20355641 None:intergenic 30.0%
ATCTAATGGAAAGCTTGTAA+TGG - chr5.3:20355342-20355361 MS.gene069146:CDS 30.0%
ATTCATTCTTCATCATATGG+AGG + chr5.3:20354445-20354464 None:intergenic 30.0%
CATATGATGAAGAATGAATG+GGG - chr5.3:20354446-20354465 MS.gene069146:CDS 30.0%
CCATATGATGAAGAATGAAT+GGG - chr5.3:20354445-20354464 MS.gene069146:CDS 30.0%
TACTTTATTCCTACGTTACT+TGG - chr5.3:20354526-20354545 MS.gene069146:CDS 30.0%
TCCATATGATGAAGAATGAA+TGG - chr5.3:20354444-20354463 MS.gene069146:CDS 30.0%
TTAGTAGCTGGAAGTTTAAT+TGG - chr5.3:20355541-20355560 MS.gene069146:intron 30.0%
! CATCCTATTTTACCATTGAT+TGG - chr5.3:20355310-20355329 MS.gene069146:intron 30.0%
! CTTCCTGTATTTTCTTTACT+TGG - chr5.3:20355391-20355410 MS.gene069146:CDS 30.0%
! TTTAAAGTTGGAGACAAAGT+AGG - chr5.3:20354705-20354724 MS.gene069146:CDS 30.0%
!! TATCGATTCGTGATTGATAT+TGG - chr5.3:20355691-20355710 MS.gene069146:CDS 30.0%
AAAGGAAGCAATAGAACACT+TGG - chr5.3:20355103-20355122 MS.gene069146:CDS 35.0%
AAGGAAGCAATAGAACACTT+GGG - chr5.3:20355104-20355123 MS.gene069146:CDS 35.0%
AATGGAAAGCTTGTAATGGT+TGG - chr5.3:20355346-20355365 MS.gene069146:CDS 35.0%
ACAACACCTATATTCATACC+AGG + chr5.3:20354993-20355012 None:intergenic 35.0%
ACATCAAGAGGTAAGTTATC+AGG + chr5.3:20354903-20354922 None:intergenic 35.0%
AGAATAACCTCCATATGTGA+CGG + chr5.3:20354841-20354860 None:intergenic 35.0%
ATATATGGTCCAAGTAACGT+AGG + chr5.3:20354538-20354557 None:intergenic 35.0%
CAATGTAAAGCCTGATATTG+AGG - chr5.3:20355609-20355628 MS.gene069146:intron 35.0%
CACATCTCGTAACAAAGAAA+AGG - chr5.3:20355085-20355104 MS.gene069146:CDS 35.0%
CCATATATCCATTAGTTCCA+GGG - chr5.3:20354474-20354493 MS.gene069146:CDS 35.0%
CCCATTCATTCTTCATCATA+TGG + chr5.3:20354448-20354467 None:intergenic 35.0%
CCTCCAAGTAAAGAAAATAC+AGG + chr5.3:20355397-20355416 None:intergenic 35.0%
GACAAACCTGGTATGAATAT+AGG - chr5.3:20354984-20355003 MS.gene069146:CDS 35.0%
GTTACGAGATGTGCTAATTA+CGG + chr5.3:20355078-20355097 None:intergenic 35.0%
TAGCTGGAAGTTTAATTGGA+GGG - chr5.3:20355545-20355564 MS.gene069146:intron 35.0%
TCCATATATCCATTAGTTCC+AGG - chr5.3:20354473-20354492 MS.gene069146:CDS 35.0%
TGAGAAAGATGTAGCATTCA+AGG - chr5.3:20354391-20354410 MS.gene069146:intron 35.0%
TGTTGCAGCCTATTAAAATG+TGG + chr5.3:20355251-20355270 None:intergenic 35.0%
! AACCTTCTGGAGAAATTGAA+AGG + chr5.3:20354174-20354193 None:intergenic 35.0%
! ACCTTCTGGAGAAATTGAAA+GGG + chr5.3:20354173-20354192 None:intergenic 35.0%
! CCTGTATTTTCTTTACTTGG+AGG - chr5.3:20355394-20355413 MS.gene069146:CDS 35.0%
! GCTGTGTTGACATAATCAAT+AGG + chr5.3:20355640-20355659 None:intergenic 35.0%
! GGTATGAATATAGGTGTTGT+TGG - chr5.3:20354993-20355012 MS.gene069146:CDS 35.0%
! TAAGTCAACTTTAGGGAAAC+TGG - chr5.3:20354368-20354387 MS.gene069146:intron 35.0%
! TATAGTCCTCTTCGACATTT+TGG - chr5.3:20354957-20354976 MS.gene069146:CDS 35.0%
!! AATATAGGTGTTGTTGGTCT+TGG - chr5.3:20354999-20355018 MS.gene069146:CDS 35.0%
!! ACATTTTAATAGGCTGCAAC+AGG - chr5.3:20355250-20355269 MS.gene069146:intron 35.0%
!! CATTCAAGGTGTTGTATTGT+GGG - chr5.3:20354405-20354424 MS.gene069146:intron 35.0%
AACTTACCTCTTGATGTTGC+CGG - chr5.3:20354906-20354925 MS.gene069146:CDS 40.0%
ACCACATGTGACTGTTTGTT+GGG + chr5.3:20354799-20354818 None:intergenic 40.0%
CACATTAGCACCAAAAGCTT+TGG + chr5.3:20355054-20355073 None:intergenic 40.0%
GTAGCTGGAAGTTTAATTGG+AGG - chr5.3:20355544-20355563 MS.gene069146:intron 40.0%
TAAATGCAGGCATGAACTTG+CGG - chr5.3:20354647-20354666 MS.gene069146:intron 40.0%
TCAAATACCTGCATCTGATC+TGG + chr5.3:20355158-20355177 None:intergenic 40.0%
TGATTATGTCAACACAGCAG+TGG - chr5.3:20355642-20355661 MS.gene069146:CDS 40.0%
TGGAGGTTATTCTGACTCAA+TGG - chr5.3:20354848-20354867 MS.gene069146:CDS 40.0%
! AGTGTTAGAGCATCCTAAGA+AGG - chr5.3:20354107-20354126 MS.gene069146:CDS 40.0%
! CCCTGGAACTAATGGATATA+TGG + chr5.3:20354477-20354496 None:intergenic 40.0%
! CCTTCTGGAGAAATTGAAAG+GGG + chr5.3:20354172-20354191 None:intergenic 40.0%
! GAAAATACAGGAAGCTCTAG+AGG + chr5.3:20355385-20355404 None:intergenic 40.0%
! GTGAAATTCGCCAAAGCTTT+TGG - chr5.3:20355041-20355060 MS.gene069146:CDS 40.0%
!! GCATTCAAGGTGTTGTATTG+TGG - chr5.3:20354404-20354423 MS.gene069146:intron 40.0%
AAATGCAGGCATGAACTTGC+GGG - chr5.3:20354648-20354667 MS.gene069146:intron 45.0%
AGTTGGAGACAAAGTAGGTG+TGG - chr5.3:20354710-20354729 MS.gene069146:CDS 45.0%
ATCCTAAGAAGGCCTTTGGT+TGG - chr5.3:20354118-20354137 MS.gene069146:CDS 45.0%
CAAAGTAGGTGTGGGATACA+TGG - chr5.3:20354719-20354738 MS.gene069146:CDS 45.0%
CACCACATGTGACTGTTTGT+TGG + chr5.3:20354800-20354819 None:intergenic 45.0%
CCCCTTTCAATTTCTCCAGA+AGG - chr5.3:20354169-20354188 MS.gene069146:CDS 45.0%
GAAGCATACCCTGGAACTAA+TGG + chr5.3:20354485-20354504 None:intergenic 45.0%
GCTGCAACAGGTACTTTGAA+TGG - chr5.3:20355262-20355281 MS.gene069146:intron 45.0%
GGAAAGCTTGTAATGGTTGG+TGG - chr5.3:20355349-20355368 MS.gene069146:CDS 45.0%
GTAGGGAGAAAGTTAGTAGC+TGG - chr5.3:20355529-20355548 MS.gene069146:intron 45.0%
GTTGGAGACAAAGTAGGTGT+GGG - chr5.3:20354711-20354730 MS.gene069146:CDS 45.0%
TCCTAAGAAGGCCTTTGGTT+GGG - chr5.3:20354119-20354138 MS.gene069146:CDS 45.0%
TCGAGTCCAAAATGTCGAAG+AGG + chr5.3:20354966-20354985 None:intergenic 45.0%
TTCCACTTTGCTTCCTACCT+CGG + chr5.3:20354682-20354701 None:intergenic 45.0%
! CACATGTGACTGTTTGTTGG+GGG + chr5.3:20354797-20354816 None:intergenic 45.0%
! CATTTTGGACTCGACAAACC+TGG - chr5.3:20354972-20354991 MS.gene069146:CDS 45.0%
! CCACATGTGACTGTTTGTTG+GGG + chr5.3:20354798-20354817 None:intergenic 45.0%
!! ATAGGTGTTGTTGGTCTTGG+TGG - chr5.3:20355002-20355021 MS.gene069146:CDS 45.0%
!! TCTTGGTGGACTTGGTCATA+TGG - chr5.3:20355016-20355035 MS.gene069146:CDS 45.0%
AAAGGGGAGAGAACACCAGA+AGG + chr5.3:20354156-20354175 None:intergenic 50.0%
AACCGAGGTAGGAAGCAAAG+TGG - chr5.3:20354677-20354696 MS.gene069146:CDS 50.0%
ACTTGCGGGTATAGTAACCG+AGG - chr5.3:20354662-20354681 MS.gene069146:CDS 50.0%
ATCTCTAGCTGCCCAACCAA+AGG + chr5.3:20354133-20354152 None:intergenic 50.0%
CCCCAACAAACAGTCACATG+TGG - chr5.3:20354795-20354814 MS.gene069146:CDS 50.0%
GAGCATCCTAAGAAGGCCTT+TGG - chr5.3:20354114-20354133 MS.gene069146:CDS 50.0%
! AAGGAGTTGGAAGCATACCC+TGG + chr5.3:20354494-20354513 None:intergenic 50.0%
! TGTGGTGCCAAGTATCGTGA+TGG - chr5.3:20354813-20354832 MS.gene069146:CDS 50.0%
!! GCTTGTAATGGTTGGTGGAG+TGG - chr5.3:20355354-20355373 MS.gene069146:CDS 50.0%
!! GTTGTTGGTCTTGGTGGACT+TGG - chr5.3:20355008-20355027 MS.gene069146:CDS 50.0%
AGAGGACCGGCAACATCAAG+AGG + chr5.3:20354915-20354934 None:intergenic 55.0%
CGTGATGGCACCGTCACATA+TGG - chr5.3:20354828-20354847 MS.gene069146:CDS 55.0%
CTGCGATCCAGATCAGATGC+AGG - chr5.3:20355148-20355167 MS.gene069146:CDS 55.0%
GATGGCACCGTCACATATGG+AGG - chr5.3:20354831-20354850 MS.gene069146:CDS 55.0%
GCCCAACCAAAGGCCTTCTT+AGG + chr5.3:20354123-20354142 None:intergenic 55.0%
GCGGGTATAGTAACCGAGGT+AGG - chr5.3:20354666-20354685 MS.gene069146:CDS 55.0%
GTGACTCCAGCGCAAAGAAG+AGG + chr5.3:20354933-20354952 None:intergenic 55.0%
TCCAGCGCAAAGAAGAGGAC+CGG + chr5.3:20354928-20354947 None:intergenic 55.0%
TGGGCAGCTAGAGATCCTTC+TGG - chr5.3:20354138-20354157 MS.gene069146:CDS 55.0%
!! GACGGTGCCATCACGATACT+TGG + chr5.3:20354823-20354842 None:intergenic 55.0%
GCCGGTCCTCTTCTTTGCGC+TGG - chr5.3:20354924-20354943 MS.gene069146:CDS 65.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 20354090 20355738 20354090 ID=MS.gene069146
chr5.3 mRNA 20354090 20355738 20354090 ID=MS.gene069146.t1;Parent=MS.gene069146
chr5.3 exon 20355638 20355738 20355638 ID=MS.gene069146.t1.exon1;Parent=MS.gene069146.t1
chr5.3 CDS 20355638 20355738 20355638 ID=cds.MS.gene069146.t1;Parent=MS.gene069146.t1
chr5.3 exon 20355333 20355446 20355333 ID=MS.gene069146.t1.exon2;Parent=MS.gene069146.t1
chr5.3 CDS 20355333 20355446 20355333 ID=cds.MS.gene069146.t1;Parent=MS.gene069146.t1
chr5.3 exon 20354659 20355172 20354659 ID=MS.gene069146.t1.exon3;Parent=MS.gene069146.t1
chr5.3 CDS 20354659 20355172 20354659 ID=cds.MS.gene069146.t1;Parent=MS.gene069146.t1
chr5.3 exon 20354413 20354566 20354413 ID=MS.gene069146.t1.exon4;Parent=MS.gene069146.t1
chr5.3 CDS 20354413 20354566 20354413 ID=cds.MS.gene069146.t1;Parent=MS.gene069146.t1
chr5.3 exon 20354090 20354295 20354090 ID=MS.gene069146.t1.exon5;Parent=MS.gene069146.t1
chr5.3 CDS 20354090 20354295 20354090 ID=cds.MS.gene069146.t1;Parent=MS.gene069146.t1
Gene Sequence

>MS.gene069146

ATGGCAACAGAAACTAAAGTGTTAGAGCATCCTAAGAAGGCCTTTGGTTGGGCAGCTAGAGATCCTTCTGGTGTTCTCTCCCCTTTCAATTTCTCCAGAAGGTTTACTTCATTATAATCAACTCATCTTTATTTAATGGATTGAAATAATATTTGATATATATATATATATGTATGTATGTATGTATGTATGTATGTATGTATGTATGTATGTATGTATGTCTGTATGTATGTATGTTTTTTTTTACTTAATTTAGATCTATGTTGTTGCCTACTAATTAAGTCAACTTTAGGGAAACTGGTGAGAAAGATGTAGCATTCAAGGTGTTGTATTGTGGGATATGTCACACTGACCTCCATATGATGAAGAATGAATGGGGCAATTCCATATATCCATTAGTTCCAGGGTATGCTTCCAACTCCTTCTACTACAATTATACTTTATTCCTACGTTACTTGGACCATATATATCTTTATACTTACTTTAATGATGAGAGAGTGAAAAGTTGAATGAGTCAATAAAAAGTAAGAGTATATTTGAAATTTGCTTATAATTAATAAATGCAGGCATGAACTTGCGGGTATAGTAACCGAGGTAGGAAGCAAAGTGGAAAAGTTTAAAGTTGGAGACAAAGTAGGTGTGGGATACATGGTTGATTCATGTCGTTCATGCGAAAATTGTGCTGACGATCTTGAGAACTACTGCCCCCAACAAACAGTCACATGTGGTGCCAAGTATCGTGATGGCACCGTCACATATGGAGGTTATTCTGACTCAATGGTTGCAGACGAGCACTTTGTGATTCGCATTCCTGATAACTTACCTCTTGATGTTGCCGGTCCTCTTCTTTGCGCTGGAGTCACAGTGTATAGTCCTCTTCGACATTTTGGACTCGACAAACCTGGTATGAATATAGGTGTTGTTGGTCTTGGTGGACTTGGTCATATGGCTGTGAAATTCGCCAAAGCTTTTGGTGCTAATGTGACCGTAATTAGCACATCTCGTAACAAAGAAAAGGAAGCAATAGAACACTTGGGAGCTGACTCGTTTCTTGTAAGCTGCGATCCAGATCAGATGCAGGTATTTGATTCATTTGATTAATTTGTGAATTTCATTATTTCAGAAATCGAGAATGTTAATCAAGAAACTAATAATGTACCACATTTTAATAGGCTGCAACAGGTACTTTGAATGGTATTATTGACACGGTTTCAGCGAGTCATCCTATTTTACCATTGATTGGTTTATTGAAATCTAATGGAAAGCTTGTAATGGTTGGTGGAGTGGCAAAGCCTCTAGAGCTTCCTGTATTTTCTTTACTTGGAGGTAAGCATATAAAAAATATTTCGATAGTATAGTTTTAAGACTAAATGCTAAATATGCAGTTTTAAATTCAAATTATGCAGAATGTTGATACTTGTATAAATTTCACATTATTTGTAGGGAGAAAGTTAGTAGCTGGAAGTTTAATTGGAGGGATAAAGGAGACTCAAGAAATGATTGATTTTGCGGCAAAACACAATGTAAAGCCTGATATTGAGGTTATTCCTATTGATTATGTCAACACAGCAGTGGAGCGTCTCGAGAAAGCAGACGTGAAATATCGATTCGTGATTGATATTGGAAATACGCTGAAACCAAGCCCTTAA
Protein sequence

>MS.gene069146.t1

MATETKVLEHPKKAFGWAARDPSGVLSPFNFSRRETGEKDVAFKVLYCGICHTDLHMMKNEWGNSIYPLVPGHELAGIVTEVGSKVEKFKVGDKVGVGYMVDSCRSCENCADDLENYCPQQTVTCGAKYRDGTVTYGGYSDSMVADEHFVIRIPDNLPLDVAGPLLCAGVTVYSPLRHFGLDKPGMNIGVVGLGGLGHMAVKFAKAFGANVTVISTSRNKEKEAIEHLGADSFLVSCDPDQMQAATGTLNGIIDTVSASHPILPLIGLLKSNGKLVMVGGVAKPLELPVFSLLGGRKLVAGSLIGGIKETQEMIDFAAKHNVKPDIEVIPIDYVNTAVERLEKADVKYRFVIDIGNTLKPSP