AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene027817

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027817.t1 MTR_5g031360 97.521 363 9 0 1 363 1 363 0.0 729
MS.gene027817.t1 MTR_5g031310 88.154 363 42 1 1 363 1 362 0.0 641
MS.gene027817.t1 MTR_5g031305 85.754 358 51 0 6 363 5 362 0.0 629
MS.gene027817.t1 MTR_5g031300 78.933 356 75 0 7 362 6 361 0.0 579
MS.gene027817.t1 MTR_5g031390 81.429 350 65 0 13 362 11 360 0.0 577
MS.gene027817.t1 MTR_4g115300 78.348 351 76 0 12 362 10 360 0.0 569
MS.gene027817.t1 MTR_5g031230 78.348 351 76 0 12 362 10 360 0.0 565
MS.gene027817.t1 MTR_5g031250 77.778 351 78 0 12 362 10 360 0.0 558
MS.gene027817.t1 MTR_5g031190 76.353 351 83 0 12 362 10 360 0.0 553
MS.gene027817.t1 MTR_5g031210 76.353 351 83 0 12 362 10 360 0.0 542
MS.gene027817.t1 MTR_8g068190 78.348 351 76 0 12 362 10 360 0.0 526
MS.gene027817.t1 MTR_5g031180 77.208 351 80 0 12 362 10 360 0.0 525
MS.gene027817.t1 MTR_8g092630 63.873 346 124 1 14 359 69 413 1.50e-152 436
MS.gene027817.t1 MTR_5g031430 60.580 345 136 0 16 360 14 358 8.80e-146 417
MS.gene027817.t1 MTR_1g025950 59.475 343 138 1 17 358 14 356 1.56e-136 393
MS.gene027817.t1 MTR_5g031250 79.741 232 47 0 12 243 10 241 1.09e-133 382
MS.gene027817.t1 MTR_1g107425 52.436 349 166 0 10 358 6 354 3.24e-125 364
MS.gene027817.t1 MTR_2g009810 53.009 349 162 2 13 360 59 406 4.68e-117 345
MS.gene027817.t1 MTR_1g107425 53.763 279 129 0 10 288 6 284 1.31e-98 295
MS.gene027817.t1 MTR_2g009820 50.628 239 115 2 125 360 16 254 1.47e-64 206
MS.gene027817.t1 MTR_5g031290 73.913 115 30 0 249 363 80 194 1.20e-52 173
MS.gene027817.t1 MTR_5g031290 80.282 71 13 1 12 81 10 80 3.77e-34 125
MS.gene027817.t1 MTR_8g095220 26.849 365 223 14 27 355 68 424 2.23e-23 100
MS.gene027817.t1 MTR_8g095220 29.583 240 142 9 27 244 68 302 5.86e-23 98.2
MS.gene027817.t1 MTR_0001s0660 27.077 325 202 11 40 333 35 355 1.70e-20 92.8
MS.gene027817.t1 MTR_5g045710 24.927 341 209 10 40 344 35 364 2.54e-16 79.7
MS.gene027817.t1 MTR_3g089977 22.478 347 234 10 41 356 37 379 9.57e-15 75.1
MS.gene027817.t1 MTR_6g465010 26.457 223 148 6 17 235 3 213 1.06e-14 74.7
MS.gene027817.t1 MTR_6g466320 26.457 223 148 6 17 235 19 229 1.19e-14 74.7
MS.gene027817.t1 MTR_6g465010 26.457 223 148 6 17 235 19 229 1.19e-14 74.7
MS.gene027817.t1 MTR_3g089940 22.024 336 230 8 41 346 37 370 3.27e-14 73.6
MS.gene027817.t1 MTR_3g089940 22.024 336 230 8 41 346 37 370 3.45e-14 73.6
MS.gene027817.t1 MTR_3g089970 21.726 336 231 7 41 346 38 371 2.16e-13 71.2
MS.gene027817.t1 MTR_7g068320 24.854 342 215 11 41 345 48 384 2.87e-13 70.9
MS.gene027817.t1 MTR_5g087710 24.348 345 213 11 41 346 46 381 7.77e-13 69.3
MS.gene027817.t1 MTR_3g079180 26.510 298 187 11 40 309 40 333 7.89e-13 69.3
MS.gene027817.t1 MTR_3g089940 23.707 232 159 3 41 255 37 267 1.09e-12 68.9
MS.gene027817.t1 MTR_3g089940 23.707 232 159 3 41 255 37 267 1.28e-12 68.6
MS.gene027817.t1 MTR_7g068330 25.731 342 211 12 41 345 41 376 1.30e-12 68.9
MS.gene027817.t1 MTR_3g099460 25.806 310 198 10 41 321 44 350 3.79e-12 67.4
MS.gene027817.t1 MTR_3g099460 25.806 310 198 10 41 321 37 343 3.79e-12 67.4
MS.gene027817.t1 MTR_5g031380 60.465 43 17 0 82 124 19 61 5.13e-12 62.4
MS.gene027817.t1 MTR_1g069430 25.397 315 204 8 41 329 36 345 1.08e-11 65.9
MS.gene027817.t1 MTR_7g099040 23.734 316 210 8 40 329 37 347 9.64e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027817.t1 AT4G37980 70.339 354 105 0 10 363 4 357 4.86e-174 488
MS.gene027817.t1 AT4G37990 70.621 354 104 0 10 363 4 357 6.09e-172 483
MS.gene027817.t1 AT4G37970 64.756 349 123 0 14 362 13 361 2.73e-167 471
MS.gene027817.t1 AT4G39330 60.286 350 139 0 12 361 10 359 1.30e-158 449
MS.gene027817.t1 AT2G21730 59.155 355 143 2 9 361 1 355 2.93e-151 431
MS.gene027817.t1 AT2G21890 58.192 354 147 1 9 361 1 354 6.64e-151 430
MS.gene027817.t1 AT4G37980 70.732 287 84 0 10 296 4 290 4.92e-133 382
MS.gene027817.t1 AT4G37970 63.251 283 104 0 14 296 13 295 3.70e-128 370
MS.gene027817.t1 AT4G39330 58.844 294 121 0 12 305 10 303 8.60e-126 364
MS.gene027817.t1 AT3G19450 53.757 346 160 0 13 358 9 354 7.61e-118 346
MS.gene027817.t1 AT1G72680 50.146 343 170 1 18 360 14 355 2.55e-112 332
MS.gene027817.t1 AT4G34230 51.445 346 168 0 13 358 8 353 1.45e-106 317
MS.gene027817.t1 AT4G34230 51.156 346 169 0 13 358 8 353 1.12e-105 315
MS.gene027817.t1 AT4G37970 62.821 234 87 0 14 247 13 246 2.21e-103 305
MS.gene027817.t1 AT4G37970 63.203 231 85 0 14 244 13 243 1.19e-102 303
MS.gene027817.t1 AT5G63620 28.571 280 170 8 27 280 70 345 2.52e-25 106
MS.gene027817.t1 AT5G63620 26.039 361 231 10 27 355 70 426 2.54e-25 106
MS.gene027817.t1 AT5G63620 29.289 239 144 7 27 244 70 304 2.22e-24 102
MS.gene027817.t1 AT1G22430 27.609 297 184 9 41 309 42 335 1.61e-18 86.7
MS.gene027817.t1 AT1G22430 27.609 297 184 9 41 309 42 335 1.61e-18 86.7
MS.gene027817.t1 AT5G43940 25.648 347 219 10 41 354 36 376 7.87e-18 84.3
MS.gene027817.t1 AT5G43940 25.648 347 219 10 41 354 48 388 7.87e-18 84.3
MS.gene027817.t1 AT1G64710 26.254 339 213 13 41 345 13 348 9.82e-18 84.0
MS.gene027817.t1 AT1G64710 25.434 346 207 13 41 345 36 371 1.21e-17 84.0
MS.gene027817.t1 AT1G32780 26.039 361 222 12 41 360 37 393 4.74e-17 82.0
MS.gene027817.t1 AT1G22440 24.058 345 215 13 41 346 40 376 1.16e-16 80.9
MS.gene027817.t1 AT5G51970 24.904 261 175 6 32 280 34 285 6.40e-16 78.6
MS.gene027817.t1 AT5G51970 24.904 261 175 6 32 280 34 285 6.40e-16 78.6
MS.gene027817.t1 AT1G22430 27.136 199 126 5 41 221 42 239 5.09e-15 75.5
MS.gene027817.t1 AT1G22430 27.136 199 126 5 41 221 42 239 8.42e-15 74.3
MS.gene027817.t1 AT1G22430 27.136 199 126 5 41 221 42 239 8.42e-15 74.3
MS.gene027817.t1 AT4G22110 24.561 342 215 14 41 345 43 378 1.03e-14 75.1
MS.gene027817.t1 AT4G22110 24.561 342 215 14 41 345 43 378 1.03e-14 75.1
MS.gene027817.t1 AT5G42250 24.716 352 223 13 41 356 45 390 1.01e-13 72.4
MS.gene027817.t1 AT1G77120 22.609 345 236 9 41 356 36 378 1.42e-13 71.6
MS.gene027817.t1 AT4G22110 25.126 199 130 6 41 221 43 240 5.44e-12 65.9

Find 93 sgRNAs with CRISPR-Local

Find 128 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGAATGTTTGAGTATATT+TGG 0.100274 5.2:+21647939 None:intergenic
TATAGTCCTCTTCGACATTT+TGG 0.197168 5.2:-21647778 MS.gene027817:CDS
GTGAAATTTGCCAAAGCTTT+TGG 0.206658 5.2:-21647694 MS.gene027817:CDS
TCAATAGCGGGAAGTTTAAT+TGG 0.224167 5.2:-21647208 MS.gene027817:CDS
TATCGATTCGTGATTGATAT+TGG 0.245698 5.2:-21647058 MS.gene027817:CDS
CCCCTTTCCATTTCTTTAGA+AGG 0.260521 5.2:-21648957 MS.gene027817:intron
TTGAATGTTTGAGTATATTT+GGG 0.265617 5.2:+21647940 None:intergenic
ATTGGTTTATTGAAATCTAA+TGG 0.271827 5.2:-21647408 MS.gene027817:CDS
AATTAGTACGTCACCTAAAA+AGG 0.277252 5.2:-21647656 MS.gene027817:CDS
CCTGATAGCTTACCTCTTGA+TGG 0.285941 5.2:-21647835 MS.gene027817:CDS
TCTTGGTGGACTTGGTCATA+TGG 0.309264 5.2:-21647719 MS.gene027817:CDS
CACTTTAGCGCCAAAAGCTT+TGG 0.345910 5.2:+21647684 None:intergenic
GCCCATCCAAAGGCCTTCTT+AGG 0.354227 5.2:+21649006 None:intergenic
GCCGGTCCTCTTCTTTGCGC+TGG 0.367778 5.2:-21647811 MS.gene027817:CDS
AAGTTTAATTGGAGGGATAA+AGG 0.369291 5.2:-21647197 MS.gene027817:CDS
CATCAAAACTTCAGGGAAAC+AGG 0.379283 5.2:-21648422 MS.gene027817:intron
AAACCAATCAATGGTAAAAC+AGG 0.382671 5.2:+21647423 None:intergenic
GTGTTTCCTAAGAAGGCCTT+TGG 0.389768 5.2:-21649012 MS.gene027817:CDS
AAGTGAACCTTCTAAAGAAA+TGG 0.391377 5.2:+21648950 None:intergenic
TAAATACAGGCACGAACTTA+CGG 0.392066 5.2:-21648088 MS.gene027817:intron
AAAACATACCCCGGGACTAA+TGG 0.397804 5.2:+21648308 None:intergenic
TTTAAAGTTGGAGACAAAGT+AGG 0.401632 5.2:-21648030 MS.gene027817:CDS
CCATCAAGAGGTAAGCTATC+AGG 0.415030 5.2:+21647835 None:intergenic
AAATGTACCTGCATCTGATC+TGG 0.416120 5.2:+21647580 None:intergenic
TGGGCAGCTAGGGACTCTTC+TGG 0.423900 5.2:-21648988 MS.gene027817:CDS
ATAGGCGTTGTTGGTCTTGG+TGG 0.424569 5.2:-21647733 MS.gene027817:CDS
AAATACAGGCACGAACTTAC+GGG 0.426387 5.2:-21648087 MS.gene027817:intron
GTTGTTGGTCTTGGTGGACT+TGG 0.427259 5.2:-21647727 MS.gene027817:CDS
CGCGATGGCACCATCACCTA+TGG 0.430946 5.2:-21647907 MS.gene027817:CDS
TTCACATTATGCAGAATGAA+TGG 0.433959 5.2:-21648346 MS.gene027817:CDS
CATTCAAGGTGTTGTATTGT+GGG 0.442854 5.2:-21648385 MS.gene027817:CDS
TTAAAGTTGGAGACAAAGTA+GGG 0.443890 5.2:-21648029 MS.gene027817:CDS
AATATAGGCGTTGTTGGTCT+TGG 0.444452 5.2:-21647736 MS.gene027817:CDS
TGAGAAAGATGTAGCATTCA+AGG 0.455978 5.2:-21648399 MS.gene027817:CDS
CAAAGTAGGGGTGGGATACA+TGG 0.457657 5.2:-21648016 MS.gene027817:CDS
TCACATTATGCAGAATGAAT+GGG 0.468998 5.2:-21648345 MS.gene027817:CDS
ACTAGTAGTGTTTCCTAAGA+AGG 0.473171 5.2:-21649019 MS.gene027817:CDS
AAATATACTCAAACATTCAA+TGG 0.483631 5.2:-21647937 MS.gene027817:CDS
AGGCCTTTGGATGGGCAGCT+AGG 0.486006 5.2:-21648999 MS.gene027817:CDS
CCTCCAAGTAAAGAAAATAC+AGG 0.487127 5.2:+21647342 None:intergenic
GATTTCAATAAACCAATCAA+TGG 0.487235 5.2:+21647414 None:intergenic
GGTATGAATATAGGCGTTGT+TGG 0.488041 5.2:-21647742 MS.gene027817:CDS
CAATGTAACTCCTGAGATTG+AGG 0.489775 5.2:-21647140 MS.gene027817:CDS
TGGAGGTTTCTCCGACTCAA+TGG 0.497539 5.2:-21647887 MS.gene027817:CDS
TCCTAAGAAGGCCTTTGGAT+GGG 0.497721 5.2:-21649007 MS.gene027817:CDS
GGCCTTTGGATGGGCAGCTA+GGG 0.497825 5.2:-21648998 MS.gene027817:CDS
AACCACCTATCCATTAGTCC+CGG 0.500566 5.2:-21648318 MS.gene027817:CDS
ATAGGAACAACCTCAATCTC+AGG 0.503943 5.2:+21647130 None:intergenic
AACCTTCTAAAGAAATGGAA+AGG 0.507463 5.2:+21648955 None:intergenic
GACAAGCCTGGTATGAATAT+AGG 0.507608 5.2:-21647751 MS.gene027817:CDS
GCAATGGAGCGTCTTGCTAA+AGG 0.517863 5.2:-21647091 MS.gene027817:CDS
AGCTTACCTCTTGATGGTGC+CGG 0.518846 5.2:-21647829 MS.gene027817:CDS
GCATTCAAGGTGTTGTATTG+TGG 0.522756 5.2:-21648386 MS.gene027817:CDS
ACCTTCTAAAGAAATGGAAA+GGG 0.523495 5.2:+21648956 None:intergenic
ACCACCTATCCATTAGTCCC+GGG 0.540962 5.2:-21648317 MS.gene027817:CDS
GAAAATACAGGAAGCTCTAG+AGG 0.546279 5.2:+21647354 None:intergenic
TTCCTAAGAAGGCCTTTGGA+TGG 0.547584 5.2:-21649008 MS.gene027817:CDS
AGTTGGAGACAAAGTAGGGG+TGG 0.553693 5.2:-21648025 MS.gene027817:CDS
TCCAGCGCAAAGAAGAGGAC+CGG 0.554636 5.2:+21647810 None:intergenic
GTGACTCCAGCGCAAAGAAG+AGG 0.566494 5.2:+21647805 None:intergenic
GTAGGGAGAAAGTCAATAGC+GGG 0.577782 5.2:-21647220 MS.gene027817:intron
GTCCCTAGCTGCCCATCCAA+AGG 0.583346 5.2:+21648996 None:intergenic
GCTGCAACGAGTACTTTGAA+TGG 0.585867 5.2:-21647474 MS.gene027817:CDS
ATCTAATGGAAAGCTTGTAA+TGG 0.587781 5.2:-21647394 MS.gene027817:CDS
CGATTATGTCAACACAGCAA+TGG 0.588395 5.2:-21647107 MS.gene027817:CDS
ATAGCGGGAAGTTTAATTGG+AGG 0.589695 5.2:-21647205 MS.gene027817:CDS
CTCATCTGCAACCATTGAGT+CGG 0.590003 5.2:+21647876 None:intergenic
TAGCGGGAAGTTTAATTGGA+GGG 0.593779 5.2:-21647204 MS.gene027817:CDS
AAGGAAGCAATAGAACACTT+AGG 0.595080 5.2:-21647631 MS.gene027817:CDS
CCGCGATCCAGATCAGATGC+AGG 0.596450 5.2:-21647587 MS.gene027817:intron
CACATTATGCAGAATGAATG+GGG 0.597941 5.2:-21648344 MS.gene027817:CDS
GGAGAAACCTCCATAGGTGA+TGG 0.604248 5.2:+21647897 None:intergenic
GTTGGAGACAAAGTAGGGGT+GGG 0.608564 5.2:-21648024 MS.gene027817:CDS
TTCAATGGTAAGTATCGCGA+TGG 0.612763 5.2:-21647922 MS.gene027817:CDS
ATACCCCGGGACTAATGGAT+AGG 0.615005 5.2:+21648313 None:intergenic
AATGGAAAGCTTGTAATGGT+TGG 0.618001 5.2:-21647390 MS.gene027817:CDS
CCCCGGGACTAATGGATAGG+TGG 0.620100 5.2:+21648316 None:intergenic
TGAATGTTTGAGTATATTTG+GGG 0.623863 5.2:+21647941 None:intergenic
ATTCATTCTGCATAATGTGA+AGG 0.626134 5.2:+21648348 None:intergenic
CCTTCTAAAGAAATGGAAAG+GGG 0.632604 5.2:+21648957 None:intergenic
TACGGGTGTAGTAACAGAGG+TGG 0.634996 5.2:-21648070 MS.gene027817:CDS
TCGAGTCCAAAATGTCGAAG+AGG 0.639224 5.2:+21647772 None:intergenic
AGAGGACCGGCACCATCAAG+AGG 0.643577 5.2:+21647823 None:intergenic
ACAACGCCTATATTCATACC+AGG 0.648632 5.2:+21647745 None:intergenic
TGAGTCGGAGAAACCTCCAT+AGG 0.648832 5.2:+21647891 None:intergenic
ACGGGTGTAGTAACAGAGGT+GGG 0.650453 5.2:-21648069 MS.gene027817:CDS
GCTGTGTTGACATAATCGAT+AGG 0.651012 5.2:+21647112 None:intergenic
TAAAGTTGGAGACAAAGTAG+GGG 0.656574 5.2:-21648028 MS.gene027817:CDS
GATGGCACCATCACCTATGG+AGG 0.673821 5.2:-21647904 MS.gene027817:CDS
CCACCTATCCATTAGTCCCG+GGG 0.685161 5.2:-21648316 MS.gene027817:intron
CCTGCATCTGATCTGGATCG+CGG 0.694160 5.2:+21647587 None:intergenic
GGTAGGGAGAAAGTCAATAG+CGG 0.705352 5.2:-21647221 MS.gene027817:intron
ACTTACGGGTGTAGTAACAG+AGG 0.741818 5.2:-21648073 MS.gene027817:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAATAATTGATAAAGTA+AGG + chr5.2:21647881-21647900 None:intergenic 10.0%
!! TAAAATAATTGATAAAGTAA+GGG + chr5.2:21647880-21647899 None:intergenic 10.0%
!! ACATTTCATTTGATTAATTT+CGG - chr5.2:21648509-21648528 MS.gene027817:intron 15.0%
!! TAAACTATAATAGAAGTTAA+AGG + chr5.2:21647795-21647814 None:intergenic 15.0%
!!! AAAGTAAAAAAGTTTAAAGT+TGG - chr5.2:21648030-21648049 MS.gene027817:CDS 15.0%
!!! AAATTTGTGTTAGTTTTATT+TGG - chr5.2:21647280-21647299 MS.gene027817:intron 15.0%
!!! AATTGCATATACTATTTTTT+TGG + chr5.2:21647374-21647393 None:intergenic 15.0%
!! AAATATACTCAAACATTCAA+TGG - chr5.2:21648135-21648154 MS.gene027817:intron 20.0%
!! AATATTGTACAACATTTCAT+AGG - chr5.2:21648576-21648595 MS.gene027817:intron 20.0%
!! AATGTATTAAATTAGTGAAG+AGG + chr5.2:21647157-21647176 None:intergenic 20.0%
!! ATAACTGATGTAATAAATAC+AGG - chr5.2:21647971-21647990 MS.gene027817:CDS 20.0%
!! ATTGAATGTTTGAGTATATT+TGG + chr5.2:21648136-21648155 None:intergenic 20.0%
!! TAGAAAATAATTGCTGAATT+TGG - chr5.2:21647946-21647965 MS.gene027817:CDS 20.0%
!! TGATGAGTAAAATAAAATGT+TGG + chr5.2:21647407-21647426 None:intergenic 20.0%
!! TTGAATGTTTGAGTATATTT+GGG + chr5.2:21648135-21648154 None:intergenic 20.0%
!! TTGTATTAATTTCGCATTAT+TGG - chr5.2:21648830-21648849 MS.gene027817:intron 20.0%
!!! ACCTTTCTTTGAAATTAAAA+GGG + chr5.2:21647217-21647236 None:intergenic 20.0%
!!! ATTGGTTTATTGAAATCTAA+TGG - chr5.2:21648664-21648683 MS.gene027817:intron 20.0%
!!! TACCTTTCTTTGAAATTAAA+AGG + chr5.2:21647218-21647237 None:intergenic 20.0%
! ACGTGACAATTAAAAAGAAA+CGG + chr5.2:21647823-21647842 None:intergenic 25.0%
! ATTAATTTCGCATTATTGGT+AGG - chr5.2:21648834-21648853 MS.gene027817:intron 25.0%
! ATTCAAAGAATGAATTGGAT+GGG - chr5.2:21647563-21647582 MS.gene027817:intron 25.0%
! CAACTATTCAAAGAATGAAT+TGG - chr5.2:21647558-21647577 MS.gene027817:intron 25.0%
! GATTTCAATAAACCAATCAA+TGG + chr5.2:21648661-21648680 None:intergenic 25.0%
! TATTCAAAGAATGAATTGGA+TGG - chr5.2:21647562-21647581 MS.gene027817:intron 25.0%
! TGAATGTTTGAGTATATTTG+GGG + chr5.2:21648134-21648153 None:intergenic 25.0%
! TTAATTTCGCATTATTGGTA+GGG - chr5.2:21648835-21648854 MS.gene027817:intron 25.0%
!!! ATGCTTTTTAAAGTACATCT+CGG - chr5.2:21647429-21647448 MS.gene027817:CDS 25.0%
!!! TCCCTTTTAATTTCAAAGAA+AGG - chr5.2:21647213-21647232 MS.gene027817:CDS 25.0%
AAACCAATCAATGGTAAAAC+AGG + chr5.2:21648652-21648671 None:intergenic 30.0%
AAGTTTAATTGGAGGGATAA+AGG - chr5.2:21648875-21648894 MS.gene027817:intron 30.0%
AATTAGTACGTCACCTAAAA+AGG - chr5.2:21648416-21648435 MS.gene027817:intron 30.0%
ATCTAATGGAAAGCTTGTAA+TGG - chr5.2:21648678-21648697 MS.gene027817:intron 30.0%
ATTCATTCTGCATAATGTGA+AGG + chr5.2:21647727-21647746 None:intergenic 30.0%
GATGTTTACATCTATGTTCT+TGG - chr5.2:21647611-21647630 MS.gene027817:CDS 30.0%
TATGATGCATCAAAACTTCA+GGG - chr5.2:21647643-21647662 MS.gene027817:CDS 30.0%
TCACATTATGCAGAATGAAT+GGG - chr5.2:21647727-21647746 MS.gene027817:CDS 30.0%
TTAAAGTTGGAGACAAAGTA+GGG - chr5.2:21648043-21648062 MS.gene027817:CDS 30.0%
TTATGATGCATCAAAACTTC+AGG - chr5.2:21647642-21647661 MS.gene027817:CDS 30.0%
TTCACATTATGCAGAATGAA+TGG - chr5.2:21647726-21647745 MS.gene027817:CDS 30.0%
! AACCTTCTAAAGAAATGGAA+AGG + chr5.2:21647120-21647139 None:intergenic 30.0%
! AAGTGAACCTTCTAAAGAAA+TGG + chr5.2:21647125-21647144 None:intergenic 30.0%
! ACCTTCTAAAGAAATGGAAA+GGG + chr5.2:21647119-21647138 None:intergenic 30.0%
! ATTGCTTCCTTTTCCTTTTT+AGG + chr5.2:21648432-21648451 None:intergenic 30.0%
! CTTCCTGTATTTTCTTTACT+TGG - chr5.2:21648727-21648746 MS.gene027817:intron 30.0%
! TTTAAAGTTGGAGACAAAGT+AGG - chr5.2:21648042-21648061 MS.gene027817:CDS 30.0%
!! TATCGATTCGTGATTGATAT+TGG - chr5.2:21649014-21649033 MS.gene027817:CDS 30.0%
!!! TGCTTTTTAAAGTACATCTC+GGG - chr5.2:21647430-21647449 MS.gene027817:CDS 30.0%
AAGGAAGCAATAGAACACTT+AGG - chr5.2:21648441-21648460 MS.gene027817:intron 35.0%
AATGGAAAGCTTGTAATGGT+TGG - chr5.2:21648682-21648701 MS.gene027817:intron 35.0%
ACTAGTAGTGTTTCCTAAGA+AGG - chr5.2:21647053-21647072 MS.gene027817:CDS 35.0%
CACATTATGCAGAATGAATG+GGG - chr5.2:21647728-21647747 MS.gene027817:CDS 35.0%
CCTCCAAGTAAAGAAAATAC+AGG + chr5.2:21648733-21648752 None:intergenic 35.0%
TAAAGTTGGAGACAAAGTAG+GGG - chr5.2:21648044-21648063 MS.gene027817:CDS 35.0%
TAAATACAGGCACGAACTTA+CGG - chr5.2:21647984-21648003 MS.gene027817:CDS 35.0%
TACGTCACCTAAAAAGGAAA+AGG - chr5.2:21648422-21648441 MS.gene027817:intron 35.0%
TGAGAAAGATGTAGCATTCA+AGG - chr5.2:21647673-21647692 MS.gene027817:CDS 35.0%
! CATCCTGTTTTACCATTGAT+TGG - chr5.2:21648646-21648665 MS.gene027817:intron 35.0%
! CCTGTATTTTCTTTACTTGG+AGG - chr5.2:21648730-21648749 MS.gene027817:intron 35.0%
! CCTTCTAAAGAAATGGAAAG+GGG + chr5.2:21647118-21647137 None:intergenic 35.0%
! GTGAAATTTGCCAAAGCTTT+TGG - chr5.2:21648378-21648397 MS.gene027817:CDS 35.0%
! TATAGTCCTCTTCGACATTT+TGG - chr5.2:21648294-21648313 MS.gene027817:intron 35.0%
! TCAATAGCGGGAAGTTTAAT+TGG - chr5.2:21648864-21648883 MS.gene027817:intron 35.0%
!! CATTCAAGGTGTTGTATTGT+GGG - chr5.2:21647687-21647706 MS.gene027817:CDS 35.0%
AAATACAGGCACGAACTTAC+GGG - chr5.2:21647985-21648004 MS.gene027817:CDS 40.0%
AAATGTACCTGCATCTGATC+TGG + chr5.2:21648495-21648514 None:intergenic 40.0%
AAGGAGTTGAAAACATACCC+CGG + chr5.2:21647776-21647795 None:intergenic 40.0%
ACAACGCCTATATTCATACC+AGG + chr5.2:21648330-21648349 None:intergenic 40.0%
ATAGGAACAACCTCAATCTC+AGG + chr5.2:21648945-21648964 None:intergenic 40.0%
CAATGTAACTCCTGAGATTG+AGG - chr5.2:21648932-21648951 MS.gene027817:intron 40.0%
CATCAAAACTTCAGGGAAAC+AGG - chr5.2:21647650-21647669 MS.gene027817:CDS 40.0%
CCCCTTTCCATTTCTTTAGA+AGG - chr5.2:21647115-21647134 MS.gene027817:CDS 40.0%
CGATTATGTCAACACAGCAA+TGG - chr5.2:21648965-21648984 MS.gene027817:CDS 40.0%
GACAAGCCTGGTATGAATAT+AGG - chr5.2:21648321-21648340 MS.gene027817:CDS 40.0%
TAGCGGGAAGTTTAATTGGA+GGG - chr5.2:21648868-21648887 MS.gene027817:intron 40.0%
! AATATAGGCGTTGTTGGTCT+TGG - chr5.2:21648336-21648355 MS.gene027817:CDS 40.0%
! ATAGCGGGAAGTTTAATTGG+AGG - chr5.2:21648867-21648886 MS.gene027817:intron 40.0%
! GAAAATACAGGAAGCTCTAG+AGG + chr5.2:21648721-21648740 None:intergenic 40.0%
! GCTGTGTTGACATAATCGAT+AGG + chr5.2:21648963-21648982 None:intergenic 40.0%
! GGTATGAATATAGGCGTTGT+TGG - chr5.2:21648330-21648349 MS.gene027817:CDS 40.0%
! TTCAATGGTAAGTATCGCGA+TGG - chr5.2:21648150-21648169 MS.gene027817:intron 40.0%
!! GCATTCAAGGTGTTGTATTG+TGG - chr5.2:21647686-21647705 MS.gene027817:CDS 40.0%
AAAACATACCCCGGGACTAA+TGG + chr5.2:21647767-21647786 None:intergenic 45.0%
AACCACCTATCCATTAGTCC+CGG - chr5.2:21647754-21647773 MS.gene027817:CDS 45.0%
ACTTACGGGTGTAGTAACAG+AGG - chr5.2:21647999-21648018 MS.gene027817:CDS 45.0%
AGGAGTTGAAAACATACCCC+GGG + chr5.2:21647775-21647794 None:intergenic 45.0%
CACTTTAGCGCCAAAAGCTT+TGG + chr5.2:21648391-21648410 None:intergenic 45.0%
CCATCAAGAGGTAAGCTATC+AGG + chr5.2:21648240-21648259 None:intergenic 45.0%
CCTGATAGCTTACCTCTTGA+TGG - chr5.2:21648237-21648256 MS.gene027817:intron 45.0%
CTCATCTGCAACCATTGAGT+CGG + chr5.2:21648199-21648218 None:intergenic 45.0%
GCTGCAACGAGTACTTTGAA+TGG - chr5.2:21648598-21648617 MS.gene027817:intron 45.0%
GGTAGGGAGAAAGTCAATAG+CGG - chr5.2:21648851-21648870 MS.gene027817:intron 45.0%
GTAGGGAGAAAGTCAATAGC+GGG - chr5.2:21648852-21648871 MS.gene027817:intron 45.0%
GTGTTTCCTAAGAAGGCCTT+TGG - chr5.2:21647060-21647079 MS.gene027817:CDS 45.0%
TCCTAAGAAGGCCTTTGGAT+GGG - chr5.2:21647065-21647084 MS.gene027817:CDS 45.0%
TCGAGTCCAAAATGTCGAAG+AGG + chr5.2:21648303-21648322 None:intergenic 45.0%
TTCCTAAGAAGGCCTTTGGA+TGG - chr5.2:21647064-21647083 MS.gene027817:CDS 45.0%
!! TCTTGGTGGACTTGGTCATA+TGG - chr5.2:21648353-21648372 MS.gene027817:CDS 45.0%
ACCACCTATCCATTAGTCCC+GGG - chr5.2:21647755-21647774 MS.gene027817:CDS 50.0%
ACGGGTGTAGTAACAGAGGT+GGG - chr5.2:21648003-21648022 MS.gene027817:CDS 50.0%
AGTTGGAGACAAAGTAGGGG+TGG - chr5.2:21648047-21648066 MS.gene027817:CDS 50.0%
CAAAGTAGGGGTGGGATACA+TGG - chr5.2:21648056-21648075 MS.gene027817:CDS 50.0%
GGAGAAACCTCCATAGGTGA+TGG + chr5.2:21648178-21648197 None:intergenic 50.0%
GTTGGAGACAAAGTAGGGGT+GGG - chr5.2:21648048-21648067 MS.gene027817:CDS 50.0%
TACGGGTGTAGTAACAGAGG+TGG - chr5.2:21648002-21648021 MS.gene027817:CDS 50.0%
TGAGTCGGAGAAACCTCCAT+AGG + chr5.2:21648184-21648203 None:intergenic 50.0%
TGGAGGTTTCTCCGACTCAA+TGG - chr5.2:21648185-21648204 MS.gene027817:intron 50.0%
! ATACCCCGGGACTAATGGAT+AGG + chr5.2:21647762-21647781 None:intergenic 50.0%
! CATTTTGGACTCGACAAGCC+TGG - chr5.2:21648309-21648328 MS.gene027817:intron 50.0%
! GCAATGGAGCGTCTTGCTAA+AGG - chr5.2:21648981-21649000 MS.gene027817:CDS 50.0%
!! AGCTTACCTCTTGATGGTGC+CGG - chr5.2:21648243-21648262 MS.gene027817:intron 50.0%
!! ATAGGCGTTGTTGGTCTTGG+TGG - chr5.2:21648339-21648358 MS.gene027817:CDS 50.0%
!! GTTGTTGGTCTTGGTGGACT+TGG - chr5.2:21648345-21648364 MS.gene027817:CDS 50.0%
CCACCTATCCATTAGTCCCG+GGG - chr5.2:21647756-21647775 MS.gene027817:CDS 55.0%
CCTGCATCTGATCTGGATCG+CGG + chr5.2:21648488-21648507 None:intergenic 55.0%
GATGGCACCATCACCTATGG+AGG - chr5.2:21648168-21648187 MS.gene027817:intron 55.0%
GCCCATCCAAAGGCCTTCTT+AGG + chr5.2:21647069-21647088 None:intergenic 55.0%
GTGACTCCAGCGCAAAGAAG+AGG + chr5.2:21648270-21648289 None:intergenic 55.0%
TCCAGCGCAAAGAAGAGGAC+CGG + chr5.2:21648265-21648284 None:intergenic 55.0%
AGAGGACCGGCACCATCAAG+AGG + chr5.2:21648252-21648271 None:intergenic 60.0%
AGGCCTTTGGATGGGCAGCT+AGG - chr5.2:21647073-21647092 MS.gene027817:CDS 60.0%
CCGCGATCCAGATCAGATGC+AGG - chr5.2:21648485-21648504 MS.gene027817:intron 60.0%
CGCGATGGCACCATCACCTA+TGG - chr5.2:21648165-21648184 MS.gene027817:intron 60.0%
GGCCTTTGGATGGGCAGCTA+GGG - chr5.2:21647074-21647093 MS.gene027817:CDS 60.0%
GTCCCTAGCTGCCCATCCAA+AGG + chr5.2:21647079-21647098 None:intergenic 60.0%
TGGGCAGCTAGGGACTCTTC+TGG - chr5.2:21647084-21647103 MS.gene027817:CDS 60.0%
! CCCCGGGACTAATGGATAGG+TGG + chr5.2:21647759-21647778 None:intergenic 60.0%
GCCGGTCCTCTTCTTTGCGC+TGG - chr5.2:21648261-21648280 MS.gene027817:intron 65.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 21647033 21649061 21647033 ID=MS.gene027817
chr5.2 mRNA 21647033 21649061 21647033 ID=MS.gene027817.t1;Parent=MS.gene027817
chr5.2 exon 21648958 21649061 21648958 ID=MS.gene027817.t1.exon1;Parent=MS.gene027817.t1
chr5.2 CDS 21648958 21649061 21648958 ID=cds.MS.gene027817.t1;Parent=MS.gene027817.t1
chr5.2 exon 21648317 21648430 21648317 ID=MS.gene027817.t1.exon2;Parent=MS.gene027817.t1
chr5.2 CDS 21648317 21648430 21648317 ID=cds.MS.gene027817.t1;Parent=MS.gene027817.t1
chr5.2 exon 21647588 21648101 21647588 ID=MS.gene027817.t1.exon3;Parent=MS.gene027817.t1
chr5.2 CDS 21647588 21648101 21647588 ID=cds.MS.gene027817.t1;Parent=MS.gene027817.t1
chr5.2 exon 21647343 21647496 21647343 ID=MS.gene027817.t1.exon4;Parent=MS.gene027817.t1
chr5.2 CDS 21647343 21647496 21647343 ID=cds.MS.gene027817.t1;Parent=MS.gene027817.t1
chr5.2 exon 21647033 21647238 21647033 ID=MS.gene027817.t1.exon5;Parent=MS.gene027817.t1
chr5.2 CDS 21647033 21647238 21647033 ID=cds.MS.gene027817.t1;Parent=MS.gene027817.t1
Gene Sequence

>MS.gene027817

ATGGCAATAGATGCTACTAAACTAGTAGTGTTTCCTAAGAAGGCCTTTGGATGGGCAGCTAGGGACTCTTCTGGTGTTCTATCCCCTTTCCATTTCTTTAGAAGGTTCACTTCACTTATCACCTCTTCACTAATTTAATACATTTTTCTAACTCATAATTTTTTTTCTACATCATTTTTTTCCCTTTTAATTTCAAAGAAAGGTAAAATCAATTTACTTTTAAAATATTTTTTATTTTTATTTTCTAAAATTTGTGTTAGTTTTATTTGGTAGCAAGAAAATAATAAACTCTTGATGTCAACAATTGTGTTCATTTTTTTTTGTAAATAATAAAAATGCCAAAAAAATAGTATATGCAATTGTATTCATAACCAACATTTTATTTTACTCATCACAATGCTTTTTAAAGTACATCTCGGGTTCTCTTATTAACAAGTTAAAATAACACGTATTTAGAAAATGAGAATAGAATAATATTTCACAAAGTAAATAGTACTTAATATTTTCATTTTATTTTTGATGTGTCAACTATTCAAAGAATGAATTGGATGGGATACGTGTTAATTGAATTAAGATTTGATGTTTACATCTATGTTCTTGGCTAACTAATTATGATGCATCAAAACTTCAGGGAAACAGGTGAGAAAGATGTAGCATTCAAGGTGTTGTATTGTGGGATATGTCACTCTGACCTTCACATTATGCAGAATGAATGGGGCAAAACCACCTATCCATTAGTCCCGGGGTATGTTTTCAACTCCTTTAACTTCTATTATAGTTTATTGCGCCGTTTCTTTTTAATTGTCACGTTTTAAGTAAAACAAATATTTTTTTATAAGTTAAACCCTTACTTTATCAATTATTTTATACTTAATATGATAACTAGTAGAGTGAGTCAATCAAAAGAAACGAGTAGAAAATAATTGCTGAATTTGGTTATAACTGATGTAATAAATACAGGCACGAACTTACGGGTGTAGTAACAGAGGTGGGAAGCAAAGTAAAAAAGTTTAAAGTTGGAGACAAAGTAGGGGTGGGATACATGGTTGATTCATGTCGTTCATGCGAAAATTGTGCTGAAGATCTTGAGAATTACTGCCCCAAATATACTCAAACATTCAATGGTAAGTATCGCGATGGCACCATCACCTATGGAGGTTTCTCCGACTCAATGGTTGCAGATGAGCACTTTGTGATTCGCATTCCTGATAGCTTACCTCTTGATGGTGCCGGTCCTCTTCTTTGCGCTGGAGTCACAGTGTATAGTCCTCTTCGACATTTTGGACTCGACAAGCCTGGTATGAATATAGGCGTTGTTGGTCTTGGTGGACTTGGTCATATGGCTGTGAAATTTGCCAAAGCTTTTGGCGCTAAAGTGACAGTAATTAGTACGTCACCTAAAAAGGAAAAGGAAGCAATAGAACACTTAGGAGCAGACTCGTTTCTTGTAAGCCGCGATCCAGATCAGATGCAGGTACATTTCATTTGATTAATTTCGGAATCTTTTGTTATTTCAGAAATTGATAATGTCAATCAAGAAACTAATATTGTACAACATTTCATAGGCTGCAACGAGTACTTTGAATGGCATTATTGACACAGTTTCAGTGAGTCATCCTGTTTTACCATTGATTGGTTTATTGAAATCTAATGGAAAGCTTGTAATGGTTGGTGCAGTAGCAAAGCCTCTAGAGCTTCCTGTATTTTCTTTACTTGGAGGTAAGCATATGAAAAATGCTTGCATATATACACACTCACAAAATGCTAATGCTAATTATGCAGAACTTTAATGCTCACTTGTATTAATTTCGCATTATTGGTAGGGAGAAAGTCAATAGCGGGAAGTTTAATTGGAGGGATAAAGGAGACTCAAGAAATGATTGATTTTGCAGCTAAACACAATGTAACTCCTGAGATTGAGGTTGTTCCTATCGATTATGTCAACACAGCAATGGAGCGTCTTGCTAAAGGAGATGTCAAATATCGATTCGTGATTGATATTGGAAATTCATTAAAACCAAGCCCTTAA
Protein sequence

>MS.gene027817.t1

MAIDATKLVVFPKKAFGWAARDSSGVLSPFHFFRRETGEKDVAFKVLYCGICHSDLHIMQNEWGKTTYPLVPGHELTGVVTEVGSKVKKFKVGDKVGVGYMVDSCRSCENCAEDLENYCPKYTQTFNGKYRDGTITYGGFSDSMVADEHFVIRIPDSLPLDGAGPLLCAGVTVYSPLRHFGLDKPGMNIGVVGLGGLGHMAVKFAKAFGAKVTVISTSPKKEKEAIEHLGADSFLVSRDPDQMQAATSTLNGIIDTVSVSHPVLPLIGLLKSNGKLVMVGAVAKPLELPVFSLLGGRKSIAGSLIGGIKETQEMIDFAAKHNVTPEIEVVPIDYVNTAMERLAKGDVKYRFVIDIGNSLKPSP