AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene069152

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069152.t1 MTR_5g031210 95.845 361 15 0 1 361 1 361 0.0 682
MS.gene069152.t1 MTR_5g031230 93.075 361 25 0 1 361 1 361 0.0 669
MS.gene069152.t1 MTR_4g115300 83.380 361 60 0 1 361 1 361 0.0 620
MS.gene069152.t1 MTR_5g031250 83.102 361 61 0 1 361 1 361 0.0 612
MS.gene069152.t1 MTR_5g031305 80.332 361 70 1 1 360 1 361 0.0 587
MS.gene069152.t1 MTR_5g031310 79.778 361 72 1 1 360 1 361 0.0 585
MS.gene069152.t1 MTR_5g031390 78.670 361 77 0 1 361 1 361 0.0 580
MS.gene069152.t1 MTR_5g031300 78.177 362 78 1 1 361 1 362 0.0 578
MS.gene069152.t1 MTR_5g031360 75.783 351 85 0 10 360 12 362 0.0 553
MS.gene069152.t1 MTR_5g031190 73.130 361 97 0 1 361 1 361 0.0 548
MS.gene069152.t1 MTR_8g068190 75.556 360 88 0 1 360 1 360 0.0 540
MS.gene069152.t1 MTR_5g031180 76.177 361 86 0 1 361 1 361 0.0 538
MS.gene069152.t1 MTR_8g092630 61.972 355 134 1 3 357 60 413 2.00e-149 428
MS.gene069152.t1 MTR_5g031250 83.817 241 39 0 1 241 1 241 1.26e-143 407
MS.gene069152.t1 MTR_5g031430 60.000 345 138 0 14 358 14 358 3.80e-141 405
MS.gene069152.t1 MTR_1g025950 59.885 349 139 1 8 355 7 355 2.34e-137 395
MS.gene069152.t1 MTR_2g009810 54.046 346 157 2 14 358 62 406 2.01e-117 346
MS.gene069152.t1 MTR_1g107425 52.507 339 161 0 15 353 13 351 1.05e-116 343
MS.gene069152.t1 MTR_1g107425 52.688 279 131 1 15 293 13 290 8.44e-92 277
MS.gene069152.t1 MTR_2g009820 50.000 242 112 2 123 358 16 254 5.45e-64 204
MS.gene069152.t1 MTR_5g031290 74.561 114 29 0 247 360 80 193 3.47e-57 184
MS.gene069152.t1 MTR_5g031290 86.250 80 10 1 1 79 1 80 4.71e-44 150
MS.gene069152.t1 MTR_0001s0660 27.723 303 193 9 38 318 35 333 6.42e-20 90.9
MS.gene069152.t1 MTR_8g095220 26.667 300 179 10 7 279 59 344 1.05e-19 90.1
MS.gene069152.t1 MTR_8g095220 27.969 261 152 9 7 245 59 305 3.87e-19 87.4
MS.gene069152.t1 MTR_3g089940 24.189 339 219 10 39 344 37 370 5.12e-16 79.0
MS.gene069152.t1 MTR_3g089940 24.189 339 219 10 39 344 37 370 5.31e-16 79.0
MS.gene069152.t1 MTR_3g089977 23.782 349 227 11 39 354 37 379 5.78e-16 79.0
MS.gene069152.t1 MTR_6g466320 25.588 340 224 11 1 325 6 331 1.11e-15 77.8
MS.gene069152.t1 MTR_6g465010 25.588 340 224 11 1 325 6 331 1.11e-15 77.8
MS.gene069152.t1 MTR_3g089970 23.599 339 221 10 39 344 38 371 5.03e-15 75.9
MS.gene069152.t1 MTR_6g465010 25.949 316 206 10 25 325 13 315 6.01e-15 75.5
MS.gene069152.t1 MTR_5g045710 26.794 209 138 3 38 233 35 241 2.64e-14 73.9
MS.gene069152.t1 MTR_3g089940 24.430 307 202 8 39 319 37 339 5.42e-14 72.8
MS.gene069152.t1 MTR_3g089940 24.430 307 202 8 39 319 37 339 6.70e-14 72.4
MS.gene069152.t1 MTR_5g031380 65.000 40 14 0 81 120 20 59 4.39e-12 62.8
MS.gene069152.t1 MTR_7g068320 26.074 349 221 14 39 353 48 393 3.11e-11 64.7
MS.gene069152.t1 MTR_3g099460 32.877 146 80 4 39 167 37 181 5.25e-11 63.9
MS.gene069152.t1 MTR_3g099460 32.877 146 80 4 39 167 44 188 5.57e-11 63.9
MS.gene069152.t1 MTR_3g099460 33.103 145 79 4 39 166 37 180 7.01e-11 63.2
MS.gene069152.t1 MTR_3g099460 33.103 145 79 4 39 166 44 187 8.61e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069152.t1 AT4G37980 68.571 350 110 0 11 360 7 356 2.14e-170 479
MS.gene069152.t1 AT4G37990 68.571 350 110 0 11 360 7 356 4.85e-166 468
MS.gene069152.t1 AT4G39330 61.560 359 138 0 1 359 1 359 1.20e-164 464
MS.gene069152.t1 AT4G37970 62.254 355 134 0 6 360 7 361 4.38e-162 458
MS.gene069152.t1 AT2G21890 61.080 352 136 1 11 361 5 356 5.40e-155 441
MS.gene069152.t1 AT2G21730 61.190 353 135 2 11 361 5 357 6.05e-154 438
MS.gene069152.t1 AT4G39330 61.794 301 115 0 1 301 1 301 1.48e-133 384
MS.gene069152.t1 AT4G37980 68.641 287 90 0 11 297 7 293 3.97e-133 382
MS.gene069152.t1 AT4G37970 61.644 292 112 0 6 297 7 298 5.51e-126 364
MS.gene069152.t1 AT1G72680 50.857 350 167 2 11 358 9 355 5.59e-115 338
MS.gene069152.t1 AT3G19450 51.156 346 169 0 11 356 9 354 7.82e-108 320
MS.gene069152.t1 AT4G34230 51.585 347 168 0 7 353 4 350 9.13e-102 305
MS.gene069152.t1 AT4G34230 51.297 347 169 0 7 353 4 350 8.01e-101 302
MS.gene069152.t1 AT4G37970 61.603 237 91 0 6 242 7 243 6.04e-99 294
MS.gene069152.t1 AT4G37970 61.603 237 91 0 6 242 7 243 8.85e-99 293
MS.gene069152.t1 AT5G63620 25.076 331 205 11 7 308 61 377 5.28e-20 91.3
MS.gene069152.t1 AT5G63620 25.333 300 183 10 7 279 61 346 7.43e-20 90.5
MS.gene069152.t1 AT5G63620 26.744 258 153 9 7 242 61 304 1.52e-19 88.6
MS.gene069152.t1 AT1G32780 27.123 365 213 16 39 358 37 393 2.84e-16 79.7
MS.gene069152.t1 AT5G51970 25.225 333 220 11 8 325 15 333 7.73e-15 75.5
MS.gene069152.t1 AT5G51970 25.225 333 220 11 8 325 15 333 7.73e-15 75.5
MS.gene069152.t1 AT1G22430 25.839 298 188 9 39 307 42 335 1.03e-14 75.1
MS.gene069152.t1 AT1G22430 25.839 298 188 9 39 307 42 335 1.03e-14 75.1
MS.gene069152.t1 AT5G43940 26.571 350 212 12 39 352 36 376 1.48e-14 74.7
MS.gene069152.t1 AT5G43940 26.857 350 211 12 39 352 48 388 1.51e-14 74.7
MS.gene069152.t1 AT1G77120 24.058 345 231 10 39 354 36 378 1.71e-14 74.3
MS.gene069152.t1 AT1G22430 26.415 212 138 4 39 233 42 252 1.37e-13 71.2
MS.gene069152.t1 AT1G22440 28.571 175 107 4 39 196 40 213 1.58e-13 71.6
MS.gene069152.t1 AT1G22430 26.415 212 138 4 39 233 42 252 1.91e-13 70.5
MS.gene069152.t1 AT1G22430 26.415 212 138 4 39 233 42 252 1.91e-13 70.5
MS.gene069152.t1 AT4G22110 25.333 300 187 11 39 307 43 336 2.01e-12 68.2
MS.gene069152.t1 AT4G22110 25.333 300 187 11 39 307 43 336 2.01e-12 68.2
MS.gene069152.t1 AT1G64710 25.000 300 195 9 39 310 13 310 2.59e-11 64.7
MS.gene069152.t1 AT1G64710 25.000 300 195 9 39 310 36 333 2.67e-11 64.7
MS.gene069152.t1 AT4G22110 25.714 175 112 3 39 196 43 216 4.68e-11 63.2

Find 96 sgRNAs with CRISPR-Local

Find 112 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCCGTCTTTCCTTTAATTT+TGG 0.091002 5.3:+20255895 MS.gene069152:CDS
TTGTTTGGATGTTCAAATTC+AGG 0.242919 5.3:-20254732 None:intergenic
GCTAGCTTACCCAAAATTAA+AGG 0.255595 5.3:-20255905 None:intergenic
ATCAACCTATCCACTAGTTC+CGG 0.329909 5.3:+20255043 MS.gene069152:CDS
CATCCTATCTTGCCACTACT+TGG 0.340131 5.3:+20255812 MS.gene069152:CDS
GCCCAACCAAAGGCCTTGTT+TGG 0.342284 5.3:-20254747 None:intergenic
GCTGCACCTCTCCTTTGTGC+TGG 0.348614 5.3:+20255425 MS.gene069152:CDS
GATGCTTCCAATTCCACTTC+CGG 0.349243 5.3:-20256029 None:intergenic
GTTGTTGGTCTTGGTGGGCT+TGG 0.363937 5.3:+20255509 MS.gene069152:CDS
GTCAAAACTTTCAGAGAAAC+AGG 0.379942 5.3:+20254939 MS.gene069152:intron
CTGGTAGCCGGAAGTGGAAT+TGG 0.383425 5.3:+20256022 MS.gene069152:CDS
GACACGAAAATTTAAGAACT+TGG 0.385264 5.3:-20256208 None:intergenic
GATATTTGACATCTGCTTTG+AGG 0.389547 5.3:-20256153 None:intergenic
CATATCGGTGTTGTTGGTCT+TGG 0.391909 5.3:+20255500 MS.gene069152:CDS
CACATTAGCACTAAAAGCTT+TGG 0.396291 5.3:-20255552 None:intergenic
GCTAGCAGATGTACTAATTA+CGG 0.396987 5.3:-20255576 None:intergenic
TCTTGGTGGGCTTGGTCACA+TGG 0.411120 5.3:+20255517 MS.gene069152:CDS
TACAGTCCTCTTAGATACTT+TGG 0.411424 5.3:+20255458 MS.gene069152:CDS
GAACATCCAAACAAGGCCTT+TGG 0.412216 5.3:+20254741 MS.gene069152:CDS
ATCGGTGTTGTTGGTCTTGG+TGG 0.419928 5.3:+20255503 MS.gene069152:CDS
CCCACATACACTACTACAAT+TGG 0.423618 5.3:+20255296 MS.gene069152:CDS
CTGCACCTCTCCTTTGTGCT+GGG 0.424848 5.3:+20255426 MS.gene069152:CDS
CGGAGGTTATTCTGACTCAT+TGG 0.425148 5.3:+20255349 MS.gene069152:CDS
ATAGTCTGCACTAGGTACTT+TGG 0.427527 5.3:+20255760 MS.gene069152:intron
TCAACCTATCCACTAGTTCC+GGG 0.430778 5.3:+20255044 MS.gene069152:CDS
TCCAAACAAGGCCTTTGGTT+GGG 0.431952 5.3:+20254746 MS.gene069152:CDS
GGTCTTCATATCGGTGTTGT+TGG 0.432442 5.3:+20255494 MS.gene069152:CDS
CCCAAAATTAAAGGAAAGAC+GGG 0.434247 5.3:-20255896 None:intergenic
CCCCTTTCAACTTCTCAAGA+AGG 0.434257 5.3:+20254796 MS.gene069152:CDS
AATCCAAGTAGTGGCAAGAT+AGG 0.440808 5.3:-20255815 None:intergenic
GACAAACCTGGTCTTCATAT+CGG 0.456603 5.3:+20255485 MS.gene069152:CDS
GGGATCTCCAGAGGCTTGTC+AGG 0.464857 5.3:-20255875 None:intergenic
GCTTCAAGTGGTAAGCCATC+AGG 0.468059 5.3:-20255401 None:intergenic
GATAGGATGATTTGCTGAAA+CGG 0.468221 5.3:-20255798 None:intergenic
AGAAAGCTGGTAGCCGGAAG+TGG 0.469070 5.3:+20256016 MS.gene069152:CDS
TTATTCTGACTCATTGGTTG+CGG 0.471466 5.3:+20255355 MS.gene069152:CDS
AACCTTCTTGAGAAGTTGAA+AGG 0.479627 5.3:-20254798 None:intergenic
ACCTTCTTGAGAAGTTGAAA+GGG 0.480830 5.3:-20254797 None:intergenic
TACATCTGCTAGCAAAGAAA+AGG 0.482166 5.3:+20255586 MS.gene069152:CDS
TATCGATTTGTGCTCGACAT+TGG 0.486206 5.3:+20256172 MS.gene069152:CDS
AAATACAGGCATGAGATTGT+TGG 0.500255 5.3:+20255149 MS.gene069152:intron
TGGTGTACCTGACAAGCCTC+TGG 0.501156 5.3:+20255868 MS.gene069152:CDS
TCTGCATGGCGAAGAATGAA+TGG 0.502931 5.3:+20255015 MS.gene069152:CDS
TCGAGTCCAAAGTATCTAAG+AGG 0.516519 5.3:-20255464 None:intergenic
ATCCAAACAAGGCCTTTGGT+TGG 0.532747 5.3:+20254745 MS.gene069152:CDS
GATGGTACCATCACGGTGCC+TGG 0.534204 5.3:-20255321 None:intergenic
GCAGGGAGAAAGCTGGTAGC+CGG 0.543654 5.3:+20256010 MS.gene069152:intron
TCGGTGTTGTTGGTCTTGGT+GGG 0.546049 5.3:+20255504 MS.gene069152:CDS
GTTGGTATAGTGACAAAGGT+GGG 0.549964 5.3:+20255167 MS.gene069152:CDS
CTGCATGGCGAAGAATGAAT+GGG 0.550333 5.3:+20255016 MS.gene069152:CDS
TGGGCAGCTAGAGACACATC+TGG 0.550336 5.3:+20254765 MS.gene069152:CDS
ACAGGAACGACTTCAATGTC+AGG 0.550360 5.3:-20256100 None:intergenic
ACCAATTGTAGTAGTGTATG+TGG 0.550386 5.3:-20255297 None:intergenic
AAAGTAGAAAAGTTCAAACT+TGG 0.551794 5.3:+20255194 MS.gene069152:CDS
CTTGGATTACTGAAGTCTCA+TGG 0.554281 5.3:+20255830 MS.gene069152:CDS
ACCCAAAATTAAAGGAAAGA+CGG 0.568562 5.3:-20255897 None:intergenic
CCAATTGTAGTAGTGTATGT+GGG 0.569144 5.3:-20255296 None:intergenic
TACGTGGTTCACATTCCTGA+TGG 0.574374 5.3:+20255386 MS.gene069152:CDS
GTCTCTAGCTGCCCAACCAA+AGG 0.577379 5.3:-20254757 None:intergenic
TACTTTGGACTCGACAAACC+TGG 0.577584 5.3:+20255473 MS.gene069152:CDS
CGTGATGGTACCATCACATA+CGG 0.582642 5.3:+20255329 MS.gene069152:CDS
CATGGAAAACTTGTAATGGT+TGG 0.586324 5.3:+20255848 MS.gene069152:CDS
GTCTCATGGAAAACTTGTAA+TGG 0.588488 5.3:+20255844 MS.gene069152:CDS
CTTGGAGACAAAGTAGGTGT+TGG 0.588558 5.3:+20255212 MS.gene069152:CDS
AAGGAAGCAAAAGAACACTT+AGG 0.590978 5.3:+20255605 MS.gene069152:CDS
AAGTGGAATTGGAAGCATCA+AGG 0.592918 5.3:+20256033 MS.gene069152:CDS
ATTATCGGCAGGGAGAAAGC+TGG 0.593426 5.3:+20256003 MS.gene069152:intron
AGAACATACCCCGGAACTAG+TGG 0.597931 5.3:-20255053 None:intergenic
ACACTACTACAATTGGTGCC+AGG 0.602754 5.3:+20255303 MS.gene069152:CDS
GACTTCAGTAATCCAAGTAG+TGG 0.605130 5.3:-20255824 None:intergenic
TGTTGGTATAGTGACAAAGG+TGG 0.606925 5.3:+20255166 MS.gene069152:CDS
TTCAAACTTGGAGACAAAGT+AGG 0.616072 5.3:+20255206 MS.gene069152:CDS
TGACATTGAAGTCGTTCCTG+TGG 0.621293 5.3:+20256102 MS.gene069152:CDS
TCGTGACCAAGATAAAATGC+AGG 0.622175 5.3:+20255649 MS.gene069152:CDS
AGAATAACCTCCGTATGTGA+TGG 0.622849 5.3:-20255339 None:intergenic
CCTTCTTGAGAAGTTGAAAG+GGG 0.624053 5.3:-20254796 None:intergenic
GTGATCCCAGCACAAAGGAG+AGG 0.625373 5.3:-20255431 None:intergenic
GCATTCAAAATATTGTATTG+TGG 0.625490 5.3:+20254975 MS.gene069152:CDS
TCTGCACTAGGTACTTTGGA+TGG 0.633602 5.3:+20255764 MS.gene069152:CDS
ATACCCCGGAACTAGTGGAT+AGG 0.638219 5.3:-20255048 None:intergenic
ATTGGTGCCAGGCACCGTGA+TGG 0.640073 5.3:+20255314 MS.gene069152:CDS
AGAGGTGCAGCAGCTTCAAG+TGG 0.642094 5.3:-20255413 None:intergenic
ATGTCACTCAGATGTCTGCA+TGG 0.642700 5.3:+20255001 MS.gene069152:CDS
CGTATGTGATGGTACCATCA+CGG 0.645343 5.3:-20255328 None:intergenic
CAATTGTAGTAGTGTATGTG+GGG 0.646114 5.3:-20255295 None:intergenic
GCTGTGTTAACATAATCCAC+AGG 0.647825 5.3:-20256118 None:intergenic
GGAAAGACGGGGATCTCCAG+AGG 0.652974 5.3:-20255884 None:intergenic
GATTGTTGGTATAGTGACAA+AGG 0.656994 5.3:+20255163 MS.gene069152:CDS
CAACCTATCCACTAGTTCCG+GGG 0.659512 5.3:+20255045 MS.gene069152:CDS
ACAACACCGATATGAAGACC+AGG 0.677179 5.3:-20255491 None:intergenic
CCAAAATTAAAGGAAAGACG+GGG 0.687666 5.3:-20255895 None:intergenic
TGAATTTGAACATCCAAACA+AGG 0.697834 5.3:+20254734 MS.gene069152:CDS
ACACAGTGATCCCAGCACAA+AGG 0.705727 5.3:-20255436 None:intergenic
TGCATGGCGAAGAATGAATG+GGG 0.734137 5.3:+20255017 MS.gene069152:CDS
GATGGTACCATCACATACGG+AGG 0.759601 5.3:+20255332 MS.gene069152:CDS
GGTTGCGGATGAGAACTACG+TGG 0.814100 5.3:+20255370 MS.gene069152:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTAATTTATATAAGAAATAC+AGG + chr5.3:20255135-20255154 MS.gene069152:intron 15.0%
!! TAGTTCAATATTCTATTATA+CGG + chr5.3:20255683-20255702 MS.gene069152:intron 15.0%
!! AATTGAAAAAAAAAGTGAAG+TGG - chr5.3:20254834-20254853 None:intergenic 20.0%
!! ACTTATATGCTAACAATTAT+CGG + chr5.3:20255988-20256007 MS.gene069152:intron 20.0%
!! CAAATCAAAATAGTAGAAAA+TGG - chr5.3:20255112-20255131 None:intergenic 20.0%
!! TTAATCATATAAGTGATCTA+TGG - chr5.3:20255950-20255969 None:intergenic 20.0%
!!! TTAAGAGTAAAATTGTTGTT+AGG + chr5.3:20254891-20254910 MS.gene069152:intron 20.0%
! AAAGTAGAAAAGTTCAAACT+TGG + chr5.3:20255194-20255213 MS.gene069152:CDS 25.0%
! AATTAGTTCACGAATTGATT+GGG - chr5.3:20255726-20255745 None:intergenic 25.0%
! AATTTAATTAGAACATACCC+CGG - chr5.3:20255065-20255084 None:intergenic 25.0%
! GCATTCAAAATATTGTATTG+TGG + chr5.3:20254975-20254994 MS.gene069152:CDS 25.0%
! TAATTAGTTCACGAATTGAT+TGG - chr5.3:20255727-20255746 None:intergenic 25.0%
ACCCAAAATTAAAGGAAAGA+CGG - chr5.3:20255900-20255919 None:intergenic 30.0%
TGAATTTGAACATCCAAACA+AGG + chr5.3:20254734-20254753 MS.gene069152:CDS 30.0%
TTGTTTGGATGTTCAAATTC+AGG - chr5.3:20254735-20254754 None:intergenic 30.0%
! TTTGTACCTGCATTTTATCT+TGG - chr5.3:20255658-20255677 None:intergenic 30.0%
!! ATGTTTTTATAGTCTGCACT+AGG + chr5.3:20255752-20255771 MS.gene069152:intron 30.0%
!! TGAAAGTTTTGACACATCAA+AGG - chr5.3:20254932-20254951 None:intergenic 30.0%
AAATACAGGCATGAGATTGT+TGG + chr5.3:20255149-20255168 MS.gene069152:intron 35.0%
AACCTTCTTGAGAAGTTGAA+AGG - chr5.3:20254801-20254820 None:intergenic 35.0%
AAGGAAGCAAAAGAACACTT+AGG + chr5.3:20255605-20255624 MS.gene069152:CDS 35.0%
ACCAATTGTAGTAGTGTATG+TGG - chr5.3:20255300-20255319 None:intergenic 35.0%
ACCTTCTTGAGAAGTTGAAA+GGG - chr5.3:20254800-20254819 None:intergenic 35.0%
AGAAACAGGTGAAAAAGATG+TGG + chr5.3:20254953-20254972 MS.gene069152:CDS 35.0%
ATATGCTAACAATTATCGGC+AGG + chr5.3:20255992-20256011 MS.gene069152:intron 35.0%
CAATTGTAGTAGTGTATGTG+GGG - chr5.3:20255298-20255317 None:intergenic 35.0%
CACATTAGCACTAAAAGCTT+TGG - chr5.3:20255555-20255574 None:intergenic 35.0%
CATGGAAAACTTGTAATGGT+TGG + chr5.3:20255848-20255867 MS.gene069152:CDS 35.0%
CCAAAATTAAAGGAAAGACG+GGG - chr5.3:20255898-20255917 None:intergenic 35.0%
CCAATTGTAGTAGTGTATGT+GGG - chr5.3:20255299-20255318 None:intergenic 35.0%
CCCAAAATTAAAGGAAAGAC+GGG - chr5.3:20255899-20255918 None:intergenic 35.0%
GATATTTGACATCTGCTTTG+AGG - chr5.3:20256156-20256175 None:intergenic 35.0%
GATTGTTGGTATAGTGACAA+AGG + chr5.3:20255163-20255182 MS.gene069152:CDS 35.0%
GCTAGCAGATGTACTAATTA+CGG - chr5.3:20255579-20255598 None:intergenic 35.0%
GTCAAAACTTTCAGAGAAAC+AGG + chr5.3:20254939-20254958 MS.gene069152:intron 35.0%
GTCTCATGGAAAACTTGTAA+TGG + chr5.3:20255844-20255863 MS.gene069152:CDS 35.0%
TACAGTCCTCTTAGATACTT+TGG + chr5.3:20255458-20255477 MS.gene069152:CDS 35.0%
TACATCTGCTAGCAAAGAAA+AGG + chr5.3:20255586-20255605 MS.gene069152:CDS 35.0%
TATGCTAACAATTATCGGCA+GGG + chr5.3:20255993-20256012 MS.gene069152:intron 35.0%
TTCAAACTTGGAGACAAAGT+AGG + chr5.3:20255206-20255225 MS.gene069152:CDS 35.0%
! GCTAGCTTACCCAAAATTAA+AGG - chr5.3:20255908-20255927 None:intergenic 35.0%
! TTATTCTGACTCATTGGTTG+CGG + chr5.3:20255355-20255374 MS.gene069152:CDS 35.0%
!! CCCGTCTTTCCTTTAATTTT+GGG + chr5.3:20255896-20255915 MS.gene069152:CDS 35.0%
!! GATAGGATGATTTGCTGAAA+CGG - chr5.3:20255801-20255820 None:intergenic 35.0%
AATCCAAGTAGTGGCAAGAT+AGG - chr5.3:20255818-20255837 None:intergenic 40.0%
AGAATAACCTCCGTATGTGA+TGG - chr5.3:20255342-20255361 None:intergenic 40.0%
ATAGTCTGCACTAGGTACTT+TGG + chr5.3:20255760-20255779 MS.gene069152:intron 40.0%
ATCAACCTATCCACTAGTTC+CGG + chr5.3:20255043-20255062 MS.gene069152:CDS 40.0%
CCCACATACACTACTACAAT+TGG + chr5.3:20255296-20255315 MS.gene069152:CDS 40.0%
CCTTCTTGAGAAGTTGAAAG+GGG - chr5.3:20254799-20254818 None:intergenic 40.0%
CTTGGATTACTGAAGTCTCA+TGG + chr5.3:20255830-20255849 MS.gene069152:CDS 40.0%
GACTTCAGTAATCCAAGTAG+TGG - chr5.3:20255827-20255846 None:intergenic 40.0%
GCTGTGTTAACATAATCCAC+AGG - chr5.3:20256121-20256140 None:intergenic 40.0%
GTTGGTATAGTGACAAAGGT+GGG + chr5.3:20255167-20255186 MS.gene069152:CDS 40.0%
TCGTGACCAAGATAAAATGC+AGG + chr5.3:20255649-20255668 MS.gene069152:CDS 40.0%
TGTTGGTATAGTGACAAAGG+TGG + chr5.3:20255166-20255185 MS.gene069152:CDS 40.0%
! GACAAACCTGGTCTTCATAT+CGG + chr5.3:20255485-20255504 MS.gene069152:CDS 40.0%
! TCGAGTCCAAAGTATCTAAG+AGG - chr5.3:20255467-20255486 None:intergenic 40.0%
!! AAGTGGAATTGGAAGCATCA+AGG + chr5.3:20256033-20256052 MS.gene069152:CDS 40.0%
!! CCCCGTCTTTCCTTTAATTT+TGG + chr5.3:20255895-20255914 MS.gene069152:CDS 40.0%
!! TATCGATTTGTGCTCGACAT+TGG + chr5.3:20256172-20256191 MS.gene069152:CDS 40.0%
ACAACACCGATATGAAGACC+AGG - chr5.3:20255494-20255513 None:intergenic 45.0%
ACAGGAACGACTTCAATGTC+AGG - chr5.3:20256103-20256122 None:intergenic 45.0%
ATCCAAACAAGGCCTTTGGT+TGG + chr5.3:20254745-20254764 MS.gene069152:CDS 45.0%
ATGTCACTCAGATGTCTGCA+TGG + chr5.3:20255001-20255020 MS.gene069152:CDS 45.0%
CATCCTATCTTGCCACTACT+TGG + chr5.3:20255812-20255831 MS.gene069152:CDS 45.0%
CCCCTTTCAACTTCTCAAGA+AGG + chr5.3:20254796-20254815 MS.gene069152:CDS 45.0%
CGGAGGTTATTCTGACTCAT+TGG + chr5.3:20255349-20255368 MS.gene069152:CDS 45.0%
CGTATGTGATGGTACCATCA+CGG - chr5.3:20255331-20255350 None:intergenic 45.0%
CGTGATGGTACCATCACATA+CGG + chr5.3:20255329-20255348 MS.gene069152:CDS 45.0%
CTGCATGGCGAAGAATGAAT+GGG + chr5.3:20255016-20255035 MS.gene069152:CDS 45.0%
CTTGGAGACAAAGTAGGTGT+TGG + chr5.3:20255212-20255231 MS.gene069152:CDS 45.0%
GAACATCCAAACAAGGCCTT+TGG + chr5.3:20254741-20254760 MS.gene069152:CDS 45.0%
GATGCTTCCAATTCCACTTC+CGG - chr5.3:20256032-20256051 None:intergenic 45.0%
TACGTGGTTCACATTCCTGA+TGG + chr5.3:20255386-20255405 MS.gene069152:CDS 45.0%
TACTTTGGACTCGACAAACC+TGG + chr5.3:20255473-20255492 MS.gene069152:CDS 45.0%
TCAACCTATCCACTAGTTCC+GGG + chr5.3:20255044-20255063 MS.gene069152:CDS 45.0%
TCCAAACAAGGCCTTTGGTT+GGG + chr5.3:20254746-20254765 MS.gene069152:CDS 45.0%
TCTGCACTAGGTACTTTGGA+TGG + chr5.3:20255764-20255783 MS.gene069152:CDS 45.0%
TCTGCATGGCGAAGAATGAA+TGG + chr5.3:20255015-20255034 MS.gene069152:CDS 45.0%
TGACATTGAAGTCGTTCCTG+TGG + chr5.3:20256102-20256121 MS.gene069152:CDS 45.0%
TGCATGGCGAAGAATGAATG+GGG + chr5.3:20255017-20255036 MS.gene069152:CDS 45.0%
! ACACTACTACAATTGGTGCC+AGG + chr5.3:20255303-20255322 MS.gene069152:CDS 45.0%
!! CATATCGGTGTTGTTGGTCT+TGG + chr5.3:20255500-20255519 MS.gene069152:CDS 45.0%
!! GGTCTTCATATCGGTGTTGT+TGG + chr5.3:20255494-20255513 MS.gene069152:CDS 45.0%
ACACAGTGATCCCAGCACAA+AGG - chr5.3:20255439-20255458 None:intergenic 50.0%
AGAACATACCCCGGAACTAG+TGG - chr5.3:20255056-20255075 None:intergenic 50.0%
ATTATCGGCAGGGAGAAAGC+TGG + chr5.3:20256003-20256022 MS.gene069152:intron 50.0%
CAACCTATCCACTAGTTCCG+GGG + chr5.3:20255045-20255064 MS.gene069152:CDS 50.0%
GATGGTACCATCACATACGG+AGG + chr5.3:20255332-20255351 MS.gene069152:CDS 50.0%
GCTTCAAGTGGTAAGCCATC+AGG - chr5.3:20255404-20255423 None:intergenic 50.0%
! ATACCCCGGAACTAGTGGAT+AGG - chr5.3:20255051-20255070 None:intergenic 50.0%
!! ATCGGTGTTGTTGGTCTTGG+TGG + chr5.3:20255503-20255522 MS.gene069152:CDS 50.0%
!! TCGGTGTTGTTGGTCTTGGT+GGG + chr5.3:20255504-20255523 MS.gene069152:CDS 50.0%
AGAAAGCTGGTAGCCGGAAG+TGG + chr5.3:20256016-20256035 MS.gene069152:CDS 55.0%
CTGCACCTCTCCTTTGTGCT+GGG + chr5.3:20255426-20255445 MS.gene069152:CDS 55.0%
CTGGTAGCCGGAAGTGGAAT+TGG + chr5.3:20256022-20256041 MS.gene069152:CDS 55.0%
GGTTGCGGATGAGAACTACG+TGG + chr5.3:20255370-20255389 MS.gene069152:CDS 55.0%
GTCTCTAGCTGCCCAACCAA+AGG - chr5.3:20254760-20254779 None:intergenic 55.0%
GTGATCCCAGCACAAAGGAG+AGG - chr5.3:20255434-20255453 None:intergenic 55.0%
TGGGCAGCTAGAGACACATC+TGG + chr5.3:20254765-20254784 MS.gene069152:CDS 55.0%
TGGTGTACCTGACAAGCCTC+TGG + chr5.3:20255868-20255887 MS.gene069152:CDS 55.0%
! AGAGGTGCAGCAGCTTCAAG+TGG - chr5.3:20255416-20255435 None:intergenic 55.0%
!! GCCCAACCAAAGGCCTTGTT+TGG - chr5.3:20254750-20254769 None:intergenic 55.0%
!! GTTGTTGGTCTTGGTGGGCT+TGG + chr5.3:20255509-20255528 MS.gene069152:CDS 55.0%
!! TCTTGGTGGGCTTGGTCACA+TGG + chr5.3:20255517-20255536 MS.gene069152:CDS 55.0%
GATGGTACCATCACGGTGCC+TGG - chr5.3:20255324-20255343 None:intergenic 60.0%
GCAGGGAGAAAGCTGGTAGC+CGG + chr5.3:20256010-20256029 MS.gene069152:intron 60.0%
GCTGCACCTCTCCTTTGTGC+TGG + chr5.3:20255425-20255444 MS.gene069152:CDS 60.0%
GGAAAGACGGGGATCTCCAG+AGG - chr5.3:20255887-20255906 None:intergenic 60.0%
GGGATCTCCAGAGGCTTGTC+AGG - chr5.3:20255878-20255897 None:intergenic 60.0%
!! ATTGGTGCCAGGCACCGTGA+TGG + chr5.3:20255314-20255333 MS.gene069152:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 20254720 20256219 20254720 ID=MS.gene069152
chr5.3 mRNA 20254720 20256219 20254720 ID=MS.gene069152.t1;Parent=MS.gene069152
chr5.3 exon 20254720 20254817 20254720 ID=MS.gene069152.t1.exon1;Parent=MS.gene069152.t1
chr5.3 CDS 20254720 20254817 20254720 ID=cds.MS.gene069152.t1;Parent=MS.gene069152.t1
chr5.3 exon 20254953 20255066 20254953 ID=MS.gene069152.t1.exon2;Parent=MS.gene069152.t1
chr5.3 CDS 20254953 20255066 20254953 ID=cds.MS.gene069152.t1;Parent=MS.gene069152.t1
chr5.3 exon 20255157 20255670 20255157 ID=MS.gene069152.t1.exon3;Parent=MS.gene069152.t1
chr5.3 CDS 20255157 20255670 20255157 ID=cds.MS.gene069152.t1;Parent=MS.gene069152.t1
chr5.3 exon 20255764 20255917 20255764 ID=MS.gene069152.t1.exon4;Parent=MS.gene069152.t1
chr5.3 CDS 20255764 20255917 20255764 ID=cds.MS.gene069152.t1;Parent=MS.gene069152.t1
chr5.3 exon 20256014 20256219 20256014 ID=MS.gene069152.t1.exon5;Parent=MS.gene069152.t1
chr5.3 CDS 20256014 20256219 20256014 ID=cds.MS.gene069152.t1;Parent=MS.gene069152.t1
Gene Sequence

>MS.gene069152

ATGGCAACACAACCTGAATTTGAACATCCAAACAAGGCCTTTGGTTGGGCAGCTAGAGACACATCTGGTGTTCTCTCCCCTTTCAACTTCTCAAGAAGGTTCATTCATACACCACTTCACTTTTTTTTTCAATTAATTTATTTTTTACACTTTGATGCATCATATATAGTGTTAAGAGTAAAATTGTTGTTAGGAAAATATATAACTCACCTTTGATGTGTCAAAACTTTCAGAGAAACAGGTGAAAAAGATGTGGCATTCAAAATATTGTATTGTGGAATATGTCACTCAGATGTCTGCATGGCGAAGAATGAATGGGGCATATCAACCTATCCACTAGTTCCGGGGTATGTTCTAATTAAATTATCTTAGAAAATTCTTAAATCTTACCATTTTCTACTATTTTGATTTGATTCTAATTTATATAAGAAATACAGGCATGAGATTGTTGGTATAGTGACAAAGGTGGGAAGCAAAGTAGAAAAGTTCAAACTTGGAGACAAAGTAGGTGTTGGCTACATAATTGATTCATGTCGATCGTGTCAAAATTGTGTTGACAATCTTGAAAATTACTGCCCCACATACACTACTACAATTGGTGCCAGGCACCGTGATGGTACCATCACATACGGAGGTTATTCTGACTCATTGGTTGCGGATGAGAACTACGTGGTTCACATTCCTGATGGCTTACCACTTGAAGCTGCTGCACCTCTCCTTTGTGCTGGGATCACTGTGTACAGTCCTCTTAGATACTTTGGACTCGACAAACCTGGTCTTCATATCGGTGTTGTTGGTCTTGGTGGGCTTGGTCACATGGCTGTGAAATTTGCCAAAGCTTTTAGTGCTAATGTGACCGTAATTAGTACATCTGCTAGCAAAGAAAAGGAAGCAAAAGAACACTTAGGAGCTGATTCATTTCTGATAAGTCGTGACCAAGATAAAATGCAGGTACAAAAACAATAGTTCAATATTCTATTATACGGAAATTTACAATGCTATTCCCAATCAATTCGTGAACTAATTATGTTAATGTTTTTATAGTCTGCACTAGGTACTTTGGATGGTATCATTGATACCGTTTCAGCAAATCATCCTATCTTGCCACTACTTGGATTACTGAAGTCTCATGGAAAACTTGTAATGGTTGGTGTACCTGACAAGCCTCTGGAGATCCCCGTCTTTCCTTTAATTTTGGGTAAGCTAGCATATATATGAACTACGTTTCCATAGATCACTTATATGATTAAAAGCTATAAGTCATATATACTTATATGCTAACAATTATCGGCAGGGAGAAAGCTGGTAGCCGGAAGTGGAATTGGAAGCATCAAGGAGACTCAAGAGATGCTTGACTTTGCTGCTAAACACAATGTAAAACCTGACATTGAAGTCGTTCCTGTGGATTATGTTAACACAGCAATTGAACGACTCCTCAAAGCAGATGTCAAATATCGATTTGTGCTCGACATTGGAAACACTCTGAAACCAAGTTCTTAA
Protein sequence

>MS.gene069152.t1

MATQPEFEHPNKAFGWAARDTSGVLSPFNFSRRETGEKDVAFKILYCGICHSDVCMAKNEWGISTYPLVPGHEIVGIVTKVGSKVEKFKLGDKVGVGYIIDSCRSCQNCVDNLENYCPTYTTTIGARHRDGTITYGGYSDSLVADENYVVHIPDGLPLEAAAPLLCAGITVYSPLRYFGLDKPGLHIGVVGLGGLGHMAVKFAKAFSANVTVISTSASKEKEAKEHLGADSFLISRDQDKMQSALGTLDGIIDTVSANHPILPLLGLLKSHGKLVMVGVPDKPLEIPVFPLILGRKLVAGSGIGSIKETQEMLDFAAKHNVKPDIEVVPVDYVNTAIERLLKADVKYRFVLDIGNTLKPSS