AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene027816

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027816.t1 MTR_5g031390 95.291 361 17 0 1 361 1 361 0.0 687
MS.gene027816.t1 MTR_5g031310 82.548 361 62 1 1 360 1 361 0.0 600
MS.gene027816.t1 MTR_5g031230 81.163 361 68 0 1 361 1 361 0.0 597
MS.gene027816.t1 MTR_5g031305 81.717 361 65 1 1 360 1 361 0.0 595
MS.gene027816.t1 MTR_5g031250 80.886 361 69 0 1 361 1 361 0.0 589
MS.gene027816.t1 MTR_4g115300 80.332 361 71 0 1 361 1 361 0.0 587
MS.gene027816.t1 MTR_5g031300 78.453 362 77 1 1 361 1 362 0.0 571
MS.gene027816.t1 MTR_5g031210 78.947 361 76 0 1 361 1 361 0.0 568
MS.gene027816.t1 MTR_5g031190 75.900 361 87 0 1 361 1 361 0.0 567
MS.gene027816.t1 MTR_5g031360 79.772 351 71 0 10 360 12 362 0.0 564
MS.gene027816.t1 MTR_5g031180 78.393 361 78 0 1 361 1 361 0.0 550
MS.gene027816.t1 MTR_8g068190 78.889 360 76 0 1 360 1 360 0.0 547
MS.gene027816.t1 MTR_8g092630 63.585 357 124 2 8 359 60 415 1.57e-152 436
MS.gene027816.t1 MTR_5g031430 60.580 345 136 0 14 358 14 358 2.20e-141 405
MS.gene027816.t1 MTR_5g031250 80.083 241 48 0 1 241 1 241 5.84e-135 385
MS.gene027816.t1 MTR_1g025950 58.046 348 145 1 9 355 8 355 9.37e-135 389
MS.gene027816.t1 MTR_1g107425 51.312 343 167 0 11 353 9 351 2.17e-118 347
MS.gene027816.t1 MTR_2g009810 52.450 347 161 3 14 358 62 406 2.07e-114 338
MS.gene027816.t1 MTR_1g107425 52.128 282 134 1 11 292 9 289 4.85e-93 280
MS.gene027816.t1 MTR_2g009820 51.489 235 105 2 130 358 23 254 1.02e-63 203
MS.gene027816.t1 MTR_5g031290 82.456 114 20 0 247 360 80 193 2.90e-61 195
MS.gene027816.t1 MTR_5g031290 85.000 80 11 1 1 79 1 80 1.01e-42 147
MS.gene027816.t1 MTR_6g466320 25.000 312 210 8 1 300 6 305 2.59e-19 88.6
MS.gene027816.t1 MTR_6g465010 25.000 312 210 8 1 300 6 305 2.59e-19 88.6
MS.gene027816.t1 MTR_6g465010 25.168 298 200 7 15 300 3 289 2.89e-19 88.2
MS.gene027816.t1 MTR_8g095220 28.755 233 142 7 10 221 51 280 3.31e-19 87.4
MS.gene027816.t1 MTR_8g095220 27.046 281 173 10 25 278 68 343 4.06e-19 88.6
MS.gene027816.t1 MTR_3g089977 24.928 349 223 10 39 354 37 379 3.25e-18 85.5
MS.gene027816.t1 MTR_3g089940 25.074 339 216 9 39 344 37 370 8.21e-18 84.3
MS.gene027816.t1 MTR_3g089940 25.074 339 216 9 39 344 37 370 8.37e-18 84.3
MS.gene027816.t1 MTR_3g089970 24.779 339 217 9 39 344 38 371 2.57e-17 82.8
MS.gene027816.t1 MTR_0001s0660 25.581 344 223 12 38 353 35 373 6.69e-16 79.0
MS.gene027816.t1 MTR_3g089940 25.333 300 194 7 39 312 37 332 2.50e-15 76.6
MS.gene027816.t1 MTR_3g089940 25.333 300 194 7 39 312 37 332 2.83e-15 76.6
MS.gene027816.t1 MTR_5g031380 57.407 54 18 1 80 128 19 72 1.02e-14 70.1
MS.gene027816.t1 MTR_7g068320 26.934 349 218 14 39 353 48 393 1.09e-13 72.0
MS.gene027816.t1 MTR_5g045710 23.411 299 200 8 38 312 35 328 1.55e-12 68.6
MS.gene027816.t1 MTR_1g069430 25.513 341 215 12 1 312 1 331 2.34e-12 67.8
MS.gene027816.t1 MTR_7g099040 24.843 318 202 10 39 327 38 347 4.45e-12 67.0
MS.gene027816.t1 MTR_3g079180 24.497 298 193 11 38 307 40 333 2.51e-11 64.7
MS.gene027816.t1 MTR_6g068930 25.515 388 237 17 2 353 4 375 4.20e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027816.t1 AT4G37980 70.000 350 105 0 11 360 7 356 5.68e-170 478
MS.gene027816.t1 AT4G37990 71.839 348 98 0 11 358 7 354 1.74e-169 477
MS.gene027816.t1 AT4G39330 61.625 357 137 0 1 357 1 357 4.01e-163 461
MS.gene027816.t1 AT4G37970 61.972 355 135 0 6 360 7 361 1.92e-158 449
MS.gene027816.t1 AT2G21890 60.807 347 135 1 12 357 6 352 4.67e-152 433
MS.gene027816.t1 AT2G21730 61.207 348 133 2 12 357 6 353 1.59e-151 432
MS.gene027816.t1 AT4G39330 61.056 303 118 0 1 303 1 303 4.21e-133 382
MS.gene027816.t1 AT4G37980 68.493 292 92 0 11 302 7 298 6.52e-131 377
MS.gene027816.t1 AT4G37970 59.589 292 118 0 6 297 7 298 3.10e-120 349
MS.gene027816.t1 AT1G72680 50.145 345 167 2 16 358 14 355 1.49e-112 332
MS.gene027816.t1 AT3G19450 51.445 346 168 0 11 356 9 354 6.95e-111 328
MS.gene027816.t1 AT4G34230 51.009 347 170 0 7 353 4 350 9.73e-103 307
MS.gene027816.t1 AT4G34230 50.720 347 171 0 7 353 4 350 8.74e-102 305
MS.gene027816.t1 AT4G37970 57.741 239 101 0 6 244 7 245 1.89e-92 277
MS.gene027816.t1 AT4G37970 58.228 237 99 0 6 242 7 243 3.15e-92 276
MS.gene027816.t1 AT5G63620 32.512 203 112 7 28 209 73 271 5.97e-23 98.2
MS.gene027816.t1 AT5G63620 27.402 281 172 9 25 278 70 345 8.29e-23 99.4
MS.gene027816.t1 AT5G63620 27.402 281 172 9 25 278 70 345 9.96e-23 99.0
MS.gene027816.t1 AT5G51970 26.909 275 178 8 39 300 43 307 7.50e-20 90.1
MS.gene027816.t1 AT5G51970 26.909 275 178 8 39 300 43 307 7.50e-20 90.1
MS.gene027816.t1 AT1G22430 26.899 316 201 12 39 327 42 354 1.06e-17 84.0
MS.gene027816.t1 AT1G22430 26.899 316 201 12 39 327 42 354 1.06e-17 84.0
MS.gene027816.t1 AT1G32780 27.174 368 209 16 39 358 37 393 4.86e-17 82.0
MS.gene027816.t1 AT5G43940 25.143 350 217 12 39 352 36 376 2.69e-16 79.7
MS.gene027816.t1 AT5G43940 25.143 350 217 12 39 352 48 388 3.06e-16 79.7
MS.gene027816.t1 AT1G22440 26.821 302 180 11 39 307 40 333 3.52e-16 79.7
MS.gene027816.t1 AT1G77120 23.590 390 250 13 1 354 1 378 1.16e-14 75.1
MS.gene027816.t1 AT1G22430 28.877 187 115 5 39 208 42 227 1.41e-13 71.2
MS.gene027816.t1 AT1G22430 28.877 187 115 5 39 208 42 227 1.71e-13 70.5
MS.gene027816.t1 AT1G22430 28.877 187 115 5 39 208 42 227 1.71e-13 70.5
MS.gene027816.t1 AT5G42250 23.154 298 196 11 39 307 45 338 8.21e-11 63.2

Find 83 sgRNAs with CRISPR-Local

Find 119 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATATTTCACATCTGCTTTA+AGG 0.136438 5.2:+21663484 None:intergenic
GCTGGTTCCATCACTATATT+TGG 0.170826 5.2:+21664450 None:intergenic
TATCGATTTGTGATTGATAT+TGG 0.198958 5.2:-21663465 MS.gene027816:CDS
TTCTTTGGATGCTCAAATTC+AGG 0.236168 5.2:+21665196 None:intergenic
GCCCAACCAAATGCCTTCTT+TGG 0.249082 5.2:+21665181 None:intergenic
GTGAAATTCGCCAAAGCTTT+TGG 0.268679 5.2:-21664229 MS.gene027816:CDS
AATTAGTACATCACCTAAAA+AGG 0.281212 5.2:-21664191 MS.gene027816:CDS
TACGAACTATCCACTAGTTT+CGG 0.319506 5.2:-21664736 MS.gene027816:CDS
ACGAACTATCCACTAGTTTC+GGG 0.335475 5.2:-21664735 MS.gene027816:CDS
TCTTGGTGGACTTGGCCATA+TGG 0.337845 5.2:-21664254 MS.gene027816:CDS
TTTAGGTGATGTACTAATTA+CGG 0.352263 5.2:+21664195 None:intergenic
CGGACGAGCACTTTGTGTTT+CGG 0.369545 5.2:-21664396 MS.gene027816:CDS
GTTGTTGGTCTTGGTGGACT+TGG 0.372585 5.2:-21664262 MS.gene027816:CDS
GAGCATCCAAAGAAGGCATT+TGG 0.380999 5.2:-21665187 MS.gene027816:CDS
ACCATCAGTGAGTGTCAATT+GGG 0.382344 5.2:+21664474 None:intergenic
TATTGTACCTTCATCTGATT+CGG 0.385508 5.2:+21664115 None:intergenic
ATAGGAATGATCTCAATATC+AGG 0.391852 5.2:+21663537 None:intergenic
CATCCTCTATTACCTCTGAT+TGG 0.394736 5.2:-21663848 MS.gene027816:CDS
AACCATCAGTGAGTGTCAAT+TGG 0.401417 5.2:+21664473 None:intergenic
ATAGGTGTTGTTGGTCTTGG+TGG 0.407624 5.2:-21664268 MS.gene027816:CDS
CACATTAGCACCAAAAGCTT+TGG 0.409643 5.2:+21664219 None:intergenic
CCCCTTTCAATTTCTCCAGA+AGG 0.415467 5.2:-21665132 MS.gene027816:intron
AATATAGGTGTTGTTGGTCT+TGG 0.416821 5.2:-21664271 MS.gene027816:CDS
GCATCAAGAGGTAACTCATC+TGG 0.417564 5.2:+21664370 None:intergenic
TGGGCAGCTAGAGACTCTTC+TGG 0.441511 5.2:-21665163 MS.gene027816:CDS
ATTGGTTTACTTAAGTCTCA+TGG 0.442120 5.2:-21663830 MS.gene027816:CDS
TGGCGAATTTCACAGCCATA+TGG 0.448868 5.2:+21664239 None:intergenic
TCCAAAGAAGGCATTTGGTT+GGG 0.449991 5.2:-21665182 MS.gene027816:CDS
TTCATAAGGCGAAGAATGAA+TGG 0.450733 5.2:-21664764 MS.gene027816:CDS
GTAGGGAGAAAGATAGTGGC+TGG 0.463044 5.2:-21663627 MS.gene027816:intron
ATATACAGACATGAAATTGT+TGG 0.463799 5.2:-21664622 MS.gene027816:intron
GGTGTGAATATAGGTGTTGT+TGG 0.468283 5.2:-21664277 MS.gene027816:CDS
GAATAATTTGTAGGCTGCAA+TGG 0.469877 5.2:-21663909 MS.gene027816:intron
AACCTTCTGGAGAAATTGAA+AGG 0.475349 5.2:+21665130 None:intergenic
GCTGTGTTGACGTAATCAAT+AGG 0.480301 5.2:+21663519 None:intergenic
ACCTTCTGGAGAAATTGAAA+GGG 0.480545 5.2:+21665131 None:intergenic
GACAAGCCTGGTGTGAATAT+AGG 0.484830 5.2:-21664286 MS.gene027816:CDS
GTAGGCTGCAATGGGTACTT+TGG 0.485806 5.2:-21663900 MS.gene027816:intron
TCATAAGGCGAAGAATGAAT+GGG 0.490720 5.2:-21664763 MS.gene027816:CDS
AATAATTTGTAGGCTGCAAT+GGG 0.491959 5.2:-21663908 MS.gene027816:intron
ATTCATTCTTCGCCTTATGA+AGG 0.497579 5.2:+21664766 None:intergenic
GCTGCTCCTCTCCTTTGTGC+TGG 0.498766 5.2:-21664346 MS.gene027816:CDS
ATGTCACTCGGACCTTCATA+AGG 0.505748 5.2:-21664778 MS.gene027816:CDS
CAGAGTAGGTGTCGGATGCT+TGG 0.505785 5.2:-21664551 MS.gene027816:CDS
ATAGTGGCTGGAAGTTTGAT+TGG 0.509085 5.2:-21663615 MS.gene027816:CDS
GTCTCATGGAAAGCTTGTAA+TGG 0.517803 5.2:-21663816 MS.gene027816:CDS
AGAAAAGCCACCATGAGTGC+TGG 0.530485 5.2:+21664432 None:intergenic
ATCCAAAGAAGGCATTTGGT+TGG 0.531418 5.2:-21665183 MS.gene027816:CDS
ATATATATACCTGCAAGTAA+AGG 0.531437 5.2:+21663755 None:intergenic
TGAATTTGAGCATCCAAAGA+AGG 0.538083 5.2:-21665194 MS.gene027816:CDS
AAACCAATCAGAGGTAATAG+AGG 0.539258 5.2:+21663845 None:intergenic
AAAGTGAGCAAGTTCAAAGT+TGG 0.541588 5.2:-21664577 MS.gene027816:CDS
AGTGATGGAACCAGCACTCA+TGG 0.542579 5.2:-21664442 MS.gene027816:CDS
GCATTAAAAGTGTTGTATTG+TGG 0.545788 5.2:-21664804 MS.gene027816:CDS
ATTGGTAGGGAGAAAGATAG+TGG 0.553404 5.2:-21663631 MS.gene027816:intron
CCCCAATTGACACTCACTGA+TGG 0.559334 5.2:-21664475 MS.gene027816:CDS
TGGCTGGAAGTTTGATTGGT+GGG 0.560766 5.2:-21663611 MS.gene027816:CDS
GCTGCAATGGGTACTTTGGA+TGG 0.565027 5.2:-21663896 MS.gene027816:CDS
GTTGGCATAGTTACAGAAGT+GGG 0.578876 5.2:-21664604 MS.gene027816:CDS
GTGGCTGGAAGTTTGATTGG+TGG 0.585158 5.2:-21663612 MS.gene027816:CDS
TCGTGATCCGAATCAGATGA+AGG 0.588520 5.2:-21664122 MS.gene027816:intron
TGTTGGCATAGTTACAGAAG+TGG 0.590305 5.2:-21664605 MS.gene027816:CDS
GATGGTTCCAAATATAGTGA+TGG 0.592641 5.2:-21664457 MS.gene027816:CDS
AAAGCTTACCCCGAAACTAG+TGG 0.599746 5.2:+21664726 None:intergenic
CATGGAAAGCTTGTAATGGT+TGG 0.604281 5.2:-21663812 MS.gene027816:CDS
ATTTCACATCTGCTTTAAGG+AGG 0.611618 5.2:+21663487 None:intergenic
ACGACTTATTAGAAATGAGT+CGG 0.622330 5.2:+21664141 None:intergenic
GTAATACCAGCACAAAGGAG+AGG 0.623289 5.2:+21664340 None:intergenic
TGATTACGTCAACACAGCGA+TGG 0.626468 5.2:-21663514 MS.gene027816:CDS
AGAGGAGCAGCAGCATCAAG+AGG 0.635270 5.2:+21664358 None:intergenic
TCGAGTTCAAAATGTCGAAG+AGG 0.636928 5.2:+21664307 None:intergenic
TTCAAAGTTGGAGACAGAGT+AGG 0.639117 5.2:-21664565 MS.gene027816:CDS
TATTATCGACACAGTTTCAG+CGG 0.643532 5.2:-21663873 MS.gene027816:CDS
GTTGGAGACAGAGTAGGTGT+CGG 0.649941 5.2:-21664559 MS.gene027816:CDS
CCTTCTGGAGAAATTGAAAG+GGG 0.653235 5.2:+21665132 None:intergenic
CCATCAGTGAGTGTCAATTG+GGG 0.658178 5.2:+21664475 None:intergenic
ACAACACCTATATTCACACC+AGG 0.672973 5.2:+21664280 None:intergenic
GACTTAAGTAAACCAATCAG+AGG 0.682500 5.2:+21663836 None:intergenic
CATAAGGCGAAGAATGAATG+GGG 0.693942 5.2:-21664762 MS.gene027816:CDS
CGAACTATCCACTAGTTTCG+GGG 0.707471 5.2:-21664734 MS.gene027816:intron
ACACAGTAATACCAGCACAA+AGG 0.710961 5.2:+21664335 None:intergenic
GATGGAACCAGCACTCATGG+TGG 0.728708 5.2:-21664439 MS.gene027816:CDS
GTATTGTGGAATATGTCACT+CGG 0.739362 5.2:-21664790 MS.gene027816:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAGTAATATAAATTGAATA+TGG + chr5.2:21663789-21663808 None:intergenic 10.0%
!!! ATATTTTGATTTATATGTTT+GGG + chr5.2:21663973-21663992 None:intergenic 10.0%
!!! ATTTATTTTAAACATTTAGA+AGG + chr5.2:21664964-21664983 None:intergenic 10.0%
!!! TATATTTTGATTTATATGTT+TGG + chr5.2:21663974-21663993 None:intergenic 10.0%
!!! TTTATTTTAAACATTTAGAA+GGG + chr5.2:21664963-21664982 None:intergenic 10.0%
!! AAATTCAATTTGATTCTAAT+TGG - chr5.2:21663993-21664012 MS.gene027816:intron 15.0%
!! ATATTATGTTTAGAAAGAAA+AGG + chr5.2:21663564-21663583 None:intergenic 15.0%
!! AAATAACATCATGAAAAAAG+TGG - chr5.2:21663699-21663718 MS.gene027816:intron 20.0%
!!! ATCAATCTAGAATAATTTGT+AGG - chr5.2:21664730-21664749 MS.gene027816:intron 20.0%
!!! ATGTTTGGGTTTTTTTTAAA+TGG + chr5.2:21663959-21663978 None:intergenic 20.0%
! AAGTATTTCACATTATTGGT+AGG - chr5.2:21665003-21665022 MS.gene027816:intron 25.0%
! AATTAGTACATCACCTAAAA+AGG - chr5.2:21664457-21664476 MS.gene027816:CDS 25.0%
! AGTATTTCACATTATTGGTA+GGG - chr5.2:21665004-21665023 MS.gene027816:intron 25.0%
! ATATACAGACATGAAATTGT+TGG - chr5.2:21664026-21664045 MS.gene027816:intron 25.0%
! ATATATATACCTGCAAGTAA+AGG + chr5.2:21664896-21664915 None:intergenic 25.0%
! ATGCTTTACATTTGTTTGTA+AGG - chr5.2:21663639-21663658 MS.gene027816:intron 25.0%
! ATTACTATGTCAAATTTCCA+GGG - chr5.2:21663801-21663820 MS.gene027816:CDS 25.0%
! GAAAAAGTGAGCAAATAAAA+TGG + chr5.2:21663613-21663632 None:intergenic 25.0%
! TATAGTTGCTTATTTAGAGA+CGG - chr5.2:21664585-21664604 MS.gene027816:CDS 25.0%
! TATTACTATGTCAAATTTCC+AGG - chr5.2:21663800-21663819 MS.gene027816:CDS 25.0%
! TCACAAGTATTTCACATTAT+TGG - chr5.2:21664999-21665018 MS.gene027816:intron 25.0%
! TCGTCAAAATTAGTAGAAAT+AGG + chr5.2:21663673-21663692 None:intergenic 25.0%
! TGAAATACTTGTGAGTTTAA+AGG + chr5.2:21664993-21665012 None:intergenic 25.0%
! TTTAGGTGATGTACTAATTA+CGG + chr5.2:21664456-21664475 None:intergenic 25.0%
!!! TATCGATTTGTGATTGATAT+TGG - chr5.2:21665183-21665202 MS.gene027816:CDS 25.0%
AATGAATGAATTGAACCTTC+TGG + chr5.2:21663534-21663553 None:intergenic 30.0%
ACGACTTATTAGAAATGAGT+CGG + chr5.2:21664510-21664529 None:intergenic 30.0%
ATAGGAATGATCTCAATATC+AGG + chr5.2:21665114-21665133 None:intergenic 30.0%
ATGATGATAATCAAACACCT+AGG + chr5.2:21663749-21663768 None:intergenic 30.0%
GATATTTCACATCTGCTTTA+AGG + chr5.2:21665167-21665186 None:intergenic 30.0%
GCATTATCTTATTATGTCCT+AGG - chr5.2:21663729-21663748 MS.gene027816:intron 30.0%
TAACATCATGAAAAAAGTGG+AGG - chr5.2:21663702-21663721 MS.gene027816:intron 30.0%
TACATCACCTAAAAAGGAAA+AGG - chr5.2:21664463-21664482 MS.gene027816:CDS 30.0%
TATTGTACCTTCATCTGATT+CGG + chr5.2:21664536-21664555 None:intergenic 30.0%
! AATAATTTGTAGGCTGCAAT+GGG - chr5.2:21664740-21664759 MS.gene027816:CDS 30.0%
! ATTGCTTCCTTTTCCTTTTT+AGG + chr5.2:21664473-21664492 None:intergenic 30.0%
! ATTGGTTTACTTAAGTCTCA+TGG - chr5.2:21664818-21664837 MS.gene027816:CDS 30.0%
! CTTTTCTAACTCAATGGTTT+CGG - chr5.2:21664232-21664251 MS.gene027816:CDS 30.0%
!! GCATTAAAAGTGTTGTATTG+TGG - chr5.2:21663844-21663863 MS.gene027816:CDS 30.0%
AAACCAATCAGAGGTAATAG+AGG + chr5.2:21664806-21664825 None:intergenic 35.0%
AAAGTGAGCAAGTTCAAAGT+TGG - chr5.2:21664071-21664090 MS.gene027816:intron 35.0%
AGGGCATATTTAGCATCATA+TGG + chr5.2:21664944-21664963 None:intergenic 35.0%
ATTCATTCTTCGCCTTATGA+AGG + chr5.2:21663885-21663904 None:intergenic 35.0%
GACTTAAGTAAACCAATCAG+AGG + chr5.2:21664815-21664834 None:intergenic 35.0%
GATGGTTCCAAATATAGTGA+TGG - chr5.2:21664191-21664210 MS.gene027816:CDS 35.0%
GTATTGTGGAATATGTCACT+CGG - chr5.2:21663858-21663877 MS.gene027816:CDS 35.0%
TATTATCGACACAGTTTCAG+CGG - chr5.2:21664775-21664794 MS.gene027816:CDS 35.0%
TCATAAGGCGAAGAATGAAT+GGG - chr5.2:21663885-21663904 MS.gene027816:CDS 35.0%
TGAATTTGAGCATCCAAAGA+AGG - chr5.2:21663454-21663473 MS.gene027816:CDS 35.0%
TTCATAAGGCGAAGAATGAA+TGG - chr5.2:21663884-21663903 MS.gene027816:CDS 35.0%
TTCTTTGGATGCTCAAATTC+AGG + chr5.2:21663455-21663474 None:intergenic 35.0%
! AACCTTCTGGAGAAATTGAA+AGG + chr5.2:21663521-21663540 None:intergenic 35.0%
! ACCTTCTGGAGAAATTGAAA+GGG + chr5.2:21663520-21663539 None:intergenic 35.0%
! GAATAATTTGTAGGCTGCAA+TGG - chr5.2:21664739-21664758 MS.gene027816:CDS 35.0%
! TACGAACTATCCACTAGTTT+CGG - chr5.2:21663912-21663931 MS.gene027816:intron 35.0%
!! AATATAGGTGTTGTTGGTCT+TGG - chr5.2:21664377-21664396 MS.gene027816:CDS 35.0%
!! ATTTCACATCTGCTTTAAGG+AGG + chr5.2:21665164-21665183 None:intergenic 35.0%
AACCATCAGTGAGTGTCAAT+TGG + chr5.2:21664178-21664197 None:intergenic 40.0%
ACAACACCTATATTCACACC+AGG + chr5.2:21664371-21664390 None:intergenic 40.0%
ACACAGTAATACCAGCACAA+AGG + chr5.2:21664316-21664335 None:intergenic 40.0%
ACCATCAGTGAGTGTCAATT+GGG + chr5.2:21664177-21664196 None:intergenic 40.0%
ATAGTGGCTGGAAGTTTGAT+TGG - chr5.2:21665033-21665052 MS.gene027816:intron 40.0%
ATTGGTAGGGAGAAAGATAG+TGG - chr5.2:21665017-21665036 MS.gene027816:intron 40.0%
CACATTAGCACCAAAAGCTT+TGG + chr5.2:21664432-21664451 None:intergenic 40.0%
CATAAGGCGAAGAATGAATG+GGG - chr5.2:21663886-21663905 MS.gene027816:CDS 40.0%
CATCCTCTATTACCTCTGAT+TGG - chr5.2:21664800-21664819 MS.gene027816:CDS 40.0%
CATGGAAAGCTTGTAATGGT+TGG - chr5.2:21664836-21664855 MS.gene027816:intron 40.0%
CCTGCAAGTAAAGGAAAAAC+AGG + chr5.2:21664887-21664906 None:intergenic 40.0%
GTCACAAAATTACTCAGCAG+CGG - chr5.2:21664680-21664699 MS.gene027816:intron 40.0%
GTCTCATGGAAAGCTTGTAA+TGG - chr5.2:21664832-21664851 MS.gene027816:intron 40.0%
GTTGGCATAGTTACAGAAGT+GGG - chr5.2:21664044-21664063 MS.gene027816:intron 40.0%
TCGAGTTCAAAATGTCGAAG+AGG + chr5.2:21664344-21664363 None:intergenic 40.0%
TGTTGGCATAGTTACAGAAG+TGG - chr5.2:21664043-21664062 MS.gene027816:intron 40.0%
TTCAAAGTTGGAGACAGAGT+AGG - chr5.2:21664083-21664102 MS.gene027816:intron 40.0%
! ACGAACTATCCACTAGTTTC+GGG - chr5.2:21663913-21663932 MS.gene027816:intron 40.0%
! CCTTCTGGAGAAATTGAAAG+GGG + chr5.2:21663519-21663538 None:intergenic 40.0%
! GCTGGTTCCATCACTATATT+TGG + chr5.2:21664201-21664220 None:intergenic 40.0%
! GCTGTGTTGACGTAATCAAT+AGG + chr5.2:21665132-21665151 None:intergenic 40.0%
! GGTGTGAATATAGGTGTTGT+TGG - chr5.2:21664371-21664390 MS.gene027816:CDS 40.0%
! GTGAAATTCGCCAAAGCTTT+TGG - chr5.2:21664419-21664438 MS.gene027816:CDS 40.0%
! TGGTGGCTTTTCTAACTCAA+TGG - chr5.2:21664226-21664245 MS.gene027816:CDS 40.0%
!! ATCCAAAGAAGGCATTTGGT+TGG - chr5.2:21663465-21663484 MS.gene027816:CDS 40.0%
!! CCTGTTTTTCCTTTACTTGC+AGG - chr5.2:21664884-21664903 MS.gene027816:intron 40.0%
!! TCCAAAGAAGGCATTTGGTT+GGG - chr5.2:21663466-21663485 MS.gene027816:CDS 40.0%
AAAGCTTACCCCGAAACTAG+TGG + chr5.2:21663925-21663944 None:intergenic 45.0%
ATGTCACTCGGACCTTCATA+AGG - chr5.2:21663870-21663889 MS.gene027816:CDS 45.0%
CCATCAGTGAGTGTCAATTG+GGG + chr5.2:21664176-21664195 None:intergenic 45.0%
CCCCTTTCAATTTCTCCAGA+AGG - chr5.2:21663516-21663535 MS.gene027816:CDS 45.0%
GACAAGCCTGGTGTGAATAT+AGG - chr5.2:21664362-21664381 MS.gene027816:CDS 45.0%
GCATCAAGAGGTAACTCATC+TGG + chr5.2:21664281-21664300 None:intergenic 45.0%
GTAATACCAGCACAAAGGAG+AGG + chr5.2:21664311-21664330 None:intergenic 45.0%
GTCTTTCTCAGATGTTTCCC+TGG + chr5.2:21663821-21663840 None:intergenic 45.0%
TCGTGATCCGAATCAGATGA+AGG - chr5.2:21664526-21664545 MS.gene027816:CDS 45.0%
TGATTACGTCAACACAGCGA+TGG - chr5.2:21665134-21665153 MS.gene027816:CDS 45.0%
TGGCGAATTTCACAGCCATA+TGG + chr5.2:21664412-21664431 None:intergenic 45.0%
TGGCTGGAAGTTTGATTGGT+GGG - chr5.2:21665037-21665056 MS.gene027816:intron 45.0%
! CATTTTGAACTCGACAAGCC+TGG - chr5.2:21664350-21664369 MS.gene027816:CDS 45.0%
! CGAACTATCCACTAGTTTCG+GGG - chr5.2:21663914-21663933 MS.gene027816:intron 45.0%
! GGAAAAACAGGAAGCTCTAG+AGG + chr5.2:21664875-21664894 None:intergenic 45.0%
!! ATAGGTGTTGTTGGTCTTGG+TGG - chr5.2:21664380-21664399 MS.gene027816:CDS 45.0%
!! GAGCATCCAAAGAAGGCATT+TGG - chr5.2:21663461-21663480 MS.gene027816:CDS 45.0%
AGAAAAGCCACCATGAGTGC+TGG + chr5.2:21664219-21664238 None:intergenic 50.0%
CCCCAATTGACACTCACTGA+TGG - chr5.2:21664173-21664192 MS.gene027816:CDS 50.0%
CGGACGAGCACTTTGTGTTT+CGG - chr5.2:21664252-21664271 MS.gene027816:CDS 50.0%
GCCCAACCAAATGCCTTCTT+TGG + chr5.2:21663470-21663489 None:intergenic 50.0%
GCTGCAATGGGTACTTTGGA+TGG - chr5.2:21664752-21664771 MS.gene027816:CDS 50.0%
GTAGGCTGCAATGGGTACTT+TGG - chr5.2:21664748-21664767 MS.gene027816:CDS 50.0%
GTGGCTGGAAGTTTGATTGG+TGG - chr5.2:21665036-21665055 MS.gene027816:intron 50.0%
GTTGGAGACAGAGTAGGTGT+CGG - chr5.2:21664089-21664108 MS.gene027816:intron 50.0%
! AGTGATGGAACCAGCACTCA+TGG - chr5.2:21664206-21664225 MS.gene027816:CDS 50.0%
! GTAGGGAGAAAGATAGTGGC+TGG - chr5.2:21665021-21665040 MS.gene027816:intron 50.0%
!! GTTGTTGGTCTTGGTGGACT+TGG - chr5.2:21664386-21664405 MS.gene027816:CDS 50.0%
!! TCTTGGTGGACTTGGCCATA+TGG - chr5.2:21664394-21664413 MS.gene027816:CDS 50.0%
!!! GGAAGCTCTAGAGGCTTTTC+AGG + chr5.2:21664866-21664885 None:intergenic 50.0%
AGAGGAGCAGCAGCATCAAG+AGG + chr5.2:21664293-21664312 None:intergenic 55.0%
GATGGAACCAGCACTCATGG+TGG - chr5.2:21664209-21664228 MS.gene027816:CDS 55.0%
TGGGCAGCTAGAGACTCTTC+TGG - chr5.2:21663485-21663504 MS.gene027816:CDS 55.0%
!! CAGAGTAGGTGTCGGATGCT+TGG - chr5.2:21664097-21664116 MS.gene027816:intron 55.0%
GCTGCTCCTCTCCTTTGTGC+TGG - chr5.2:21664302-21664321 MS.gene027816:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 21663440 21665230 21663440 ID=MS.gene027816
chr5.2 mRNA 21663440 21665230 21663440 ID=MS.gene027816.t1;Parent=MS.gene027816
chr5.2 exon 21665133 21665230 21665133 ID=MS.gene027816.t1.exon1;Parent=MS.gene027816.t1
chr5.2 CDS 21665133 21665230 21665133 ID=cds.MS.gene027816.t1;Parent=MS.gene027816.t1
chr5.2 exon 21664735 21664848 21664735 ID=MS.gene027816.t1.exon2;Parent=MS.gene027816.t1
chr5.2 CDS 21664735 21664848 21664735 ID=cds.MS.gene027816.t1;Parent=MS.gene027816.t1
chr5.2 exon 21664123 21664636 21664123 ID=MS.gene027816.t1.exon3;Parent=MS.gene027816.t1
chr5.2 CDS 21664123 21664636 21664123 ID=cds.MS.gene027816.t1;Parent=MS.gene027816.t1
chr5.2 exon 21663765 21663918 21663765 ID=MS.gene027816.t1.exon4;Parent=MS.gene027816.t1
chr5.2 CDS 21663765 21663918 21663765 ID=cds.MS.gene027816.t1;Parent=MS.gene027816.t1
chr5.2 exon 21663440 21663645 21663440 ID=MS.gene027816.t1.exon5;Parent=MS.gene027816.t1
chr5.2 CDS 21663440 21663645 21663440 ID=cds.MS.gene027816.t1;Parent=MS.gene027816.t1
Gene Sequence

>MS.gene027816

ATGGCAACACAACCTGAATTTGAGCATCCAAAGAAGGCATTTGGTTGGGCAGCTAGAGACTCTTCTGGTGTTCTCTCCCCTTTCAATTTCTCCAGAAGGTTCAATTCATTCATTATGATCACCTTTTCTTTCTAAACATAATATGCATGTAGTATTCATTATTTGTTGCACCATTTTATTTGCTCACTTTTTCTTTATAATGCTTTACATTTGTTTGTAAGGTGTTATTTCCTATTTCTACTAATTTTGACGAAAATATAAATAACATCATGAAAAAAGTGGAGGATATGCATTATCTTATTATGTCCTAGGTGTTTGATTATCATCATTATATAGAGATAACTAACCATATTCAATTTATATTACTATGTCAAATTTCCAGGGAAACATCTGAGAAAGACGTAGCATTAAAAGTGTTGTATTGTGGAATATGTCACTCGGACCTTCATAAGGCGAAGAATGAATGGGGCACTACGAACTATCCACTAGTTTCGGGGTAAGCTTTCTACTTTTATTCCATTTAAAAAAAACCCAAACATATAAATCAAAATATAAATTCAATTTGATTCTAATTGGTGTAAGAAATATATACAGACATGAAATTGTTGGCATAGTTACAGAAGTGGGAAGCAAAGTGAGCAAGTTCAAAGTTGGAGACAGAGTAGGTGTCGGATGCTTGGTTGATTCTTGTCACTCATGTCAAAATTGTGTTGATAATCTTGAGAATTACTGTCCCCAATTGACACTCACTGATGGTTCCAAATATAGTGATGGAACCAGCACTCATGGTGGCTTTTCTAACTCAATGGTTTCGGACGAGCACTTTGTGTTTCGGATTCCAGATGAGTTACCTCTTGATGCTGCTGCTCCTCTCCTTTGTGCTGGTATTACTGTGTATAGTCCTCTTCGACATTTTGAACTCGACAAGCCTGGTGTGAATATAGGTGTTGTTGGTCTTGGTGGACTTGGCCATATGGCTGTGAAATTCGCCAAAGCTTTTGGTGCTAATGTGACCGTAATTAGTACATCACCTAAAAAGGAAAAGGAAGCAATAGAAAACTTAAGAGCCGACTCATTTCTAATAAGTCGTGATCCGAATCAGATGAAGGTACAATAATTAATTTAATTCACTATAAAAATTGTCATATAGTTGCTTATTTAGAGACGGAAAATGTCAAATCGATCACTAAATTAAATTGATATTTAACATTTGTCATTAGTGACTAGTAGTCTCTAATCAGTCACAAAATTACTCAGCAGCGGATTTAGAAGTTGACAATTAGTGAACTAATCAATCTAGAATAATTTGTAGGCTGCAATGGGTACTTTGGATGGTATTATCGACACAGTTTCAGCGGATCATCCTCTATTACCTCTGATTGGTTTACTTAAGTCTCATGGAAAGCTTGTAATGGTTGGTGCACCTGAAAAGCCTCTAGAGCTTCCTGTTTTTCCTTTACTTGCAGGTATATATATAAGCATTTGAAAAATATATATACTTCCATATGATGCTAAATATGCCCTTCTAAATGTTTAAAATAAATTATGAACCTTTAAACTCACAAGTATTTCACATTATTGGTAGGGAGAAAGATAGTGGCTGGAAGTTTGATTGGTGGGATCAAAGAGACTCAAGAAATGATTGATTTTGCAGCAGAACACAATGTAAAGCCTGATATTGAGATCATTCCTATTGATTACGTCAACACAGCGATGGAGCGCCTCCTTAAAGCAGATGTGAAATATCGATTTGTGATTGATATTGGAAACACATTAAAATCAAGCTCTTGA
Protein sequence

>MS.gene027816.t1

MATQPEFEHPKKAFGWAARDSSGVLSPFNFSRRETSEKDVALKVLYCGICHSDLHKAKNEWGTTNYPLVSGHEIVGIVTEVGSKVSKFKVGDRVGVGCLVDSCHSCQNCVDNLENYCPQLTLTDGSKYSDGTSTHGGFSNSMVSDEHFVFRIPDELPLDAAAPLLCAGITVYSPLRHFELDKPGVNIGVVGLGGLGHMAVKFAKAFGANVTVISTSPKKEKEAIENLRADSFLISRDPNQMKAAMGTLDGIIDTVSADHPLLPLIGLLKSHGKLVMVGAPEKPLELPVFPLLAGRKIVAGSLIGGIKETQEMIDFAAEHNVKPDIEIIPIDYVNTAMERLLKADVKYRFVIDIGNTLKSSS