AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene070869

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070869.t1 MTR_5g031210 96.399 361 13 0 1 361 1 361 0.0 719
MS.gene070869.t1 MTR_5g031230 93.629 361 23 0 1 361 1 361 0.0 672
MS.gene070869.t1 MTR_4g115300 83.657 361 59 0 1 361 1 361 0.0 622
MS.gene070869.t1 MTR_5g031250 83.657 361 59 0 1 361 1 361 0.0 614
MS.gene070869.t1 MTR_5g031310 80.055 361 71 1 1 360 1 361 0.0 587
MS.gene070869.t1 MTR_5g031305 80.332 361 70 1 1 360 1 361 0.0 586
MS.gene070869.t1 MTR_5g031390 78.947 361 76 0 1 361 1 361 0.0 582
MS.gene070869.t1 MTR_5g031300 78.729 362 76 1 1 361 1 362 0.0 581
MS.gene070869.t1 MTR_5g031360 76.353 351 83 0 10 360 12 362 0.0 555
MS.gene070869.t1 MTR_5g031190 73.961 361 94 0 1 361 1 361 0.0 551
MS.gene070869.t1 MTR_8g068190 76.111 360 86 0 1 360 1 360 0.0 543
MS.gene070869.t1 MTR_5g031180 76.731 361 84 0 1 361 1 361 0.0 541
MS.gene070869.t1 MTR_8g092630 62.712 354 131 1 4 357 61 413 1.16e-150 431
MS.gene070869.t1 MTR_5g031250 84.647 241 37 0 1 241 1 241 8.15e-145 410
MS.gene070869.t1 MTR_5g031430 60.000 345 138 0 14 358 14 358 1.26e-140 403
MS.gene070869.t1 MTR_1g025950 60.057 348 138 1 9 355 8 355 4.55e-138 397
MS.gene070869.t1 MTR_2g009810 54.624 346 155 2 14 358 62 406 1.16e-118 349
MS.gene070869.t1 MTR_1g107425 52.802 339 160 0 15 353 13 351 1.02e-117 345
MS.gene070869.t1 MTR_1g107425 53.047 279 130 1 15 293 13 290 8.17e-93 280
MS.gene070869.t1 MTR_2g009820 50.413 242 111 2 123 358 16 254 7.33e-65 206
MS.gene070869.t1 MTR_5g031290 74.561 114 29 0 247 360 80 193 3.51e-57 184
MS.gene070869.t1 MTR_5g031290 86.250 80 10 1 1 79 1 80 4.91e-44 150
MS.gene070869.t1 MTR_8g095220 27.333 300 177 10 7 279 59 344 7.11e-21 93.6
MS.gene070869.t1 MTR_8g095220 28.736 261 150 9 7 245 59 305 2.14e-20 90.9
MS.gene070869.t1 MTR_0001s0660 27.393 303 194 9 38 318 35 333 1.85e-19 89.7
MS.gene070869.t1 MTR_6g466320 26.176 340 222 11 1 325 6 331 8.93e-17 80.9
MS.gene070869.t1 MTR_6g465010 26.176 340 222 11 1 325 6 331 8.93e-17 80.9
MS.gene070869.t1 MTR_3g089977 23.496 349 228 11 39 354 37 379 3.52e-16 79.3
MS.gene070869.t1 MTR_3g089940 23.894 339 220 10 39 344 37 370 3.59e-16 79.3
MS.gene070869.t1 MTR_3g089940 23.894 339 220 10 39 344 37 370 4.02e-16 79.3
MS.gene070869.t1 MTR_6g465010 26.582 316 204 10 25 325 13 315 4.59e-16 79.0
MS.gene070869.t1 MTR_3g089970 23.599 339 221 10 39 344 38 371 6.94e-16 78.6
MS.gene070869.t1 MTR_3g089940 24.104 307 203 8 39 319 37 339 3.50e-14 73.2
MS.gene070869.t1 MTR_3g089940 24.104 307 203 8 39 319 37 339 3.95e-14 73.2
MS.gene070869.t1 MTR_5g045710 26.316 209 139 3 38 233 35 241 6.82e-14 72.4
MS.gene070869.t1 MTR_7g068320 26.648 349 219 14 39 353 48 393 5.61e-12 67.0
MS.gene070869.t1 MTR_5g031380 62.500 40 15 0 81 120 20 59 1.50e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070869.t1 AT4G37980 69.714 350 106 0 11 360 7 356 2.92e-173 486
MS.gene070869.t1 AT4G37990 68.571 350 110 0 11 360 7 356 1.42e-166 469
MS.gene070869.t1 AT4G39330 61.281 359 139 0 1 359 1 359 1.72e-164 464
MS.gene070869.t1 AT4G37970 62.817 355 132 0 6 360 7 361 3.77e-164 463
MS.gene070869.t1 AT2G21890 60.795 352 137 1 11 361 5 356 4.15e-155 441
MS.gene070869.t1 AT2G21730 60.907 353 136 2 11 361 5 357 7.13e-154 438
MS.gene070869.t1 AT4G37980 70.035 287 86 0 11 297 7 293 6.09e-136 389
MS.gene070869.t1 AT4G39330 61.462 301 116 0 1 301 1 301 1.70e-133 384
MS.gene070869.t1 AT4G37970 62.329 292 110 0 6 297 7 298 5.68e-128 369
MS.gene070869.t1 AT1G72680 51.429 350 165 2 11 358 9 355 2.80e-116 342
MS.gene070869.t1 AT3G19450 51.734 346 167 0 11 356 9 354 3.20e-109 324
MS.gene070869.t1 AT4G34230 52.161 347 166 0 7 353 4 350 1.19e-103 309
MS.gene070869.t1 AT4G34230 51.873 347 167 0 7 353 4 350 9.84e-103 307
MS.gene070869.t1 AT4G37970 62.447 237 89 0 6 242 7 243 4.80e-101 299
MS.gene070869.t1 AT4G37970 62.447 237 89 0 6 242 7 243 7.66e-101 298
MS.gene070869.t1 AT5G63620 25.076 331 205 11 7 308 61 377 1.53e-20 92.8
MS.gene070869.t1 AT5G63620 25.333 300 183 10 7 279 61 346 2.07e-20 92.4
MS.gene070869.t1 AT5G63620 26.744 258 153 9 7 242 61 304 4.23e-20 90.1
MS.gene070869.t1 AT1G32780 26.849 365 214 16 39 358 37 393 1.70e-16 80.5
MS.gene070869.t1 AT5G43940 27.143 350 210 12 39 352 48 388 1.31e-15 77.8
MS.gene070869.t1 AT5G43940 26.857 350 211 12 39 352 36 376 1.37e-15 77.8
MS.gene070869.t1 AT1G77120 24.058 345 231 10 39 354 36 378 4.17e-15 76.3
MS.gene070869.t1 AT5G51970 25.526 333 219 11 8 325 15 333 4.59e-15 75.9
MS.gene070869.t1 AT5G51970 25.526 333 219 11 8 325 15 333 4.59e-15 75.9
MS.gene070869.t1 AT1G22430 25.503 298 189 9 39 307 42 335 5.03e-15 76.3
MS.gene070869.t1 AT1G22430 25.503 298 189 9 39 307 42 335 5.03e-15 76.3
MS.gene070869.t1 AT1G22430 25.943 212 139 4 39 233 42 252 5.78e-14 72.4
MS.gene070869.t1 AT1G22430 25.943 212 139 4 39 233 42 252 8.42e-14 71.6
MS.gene070869.t1 AT1G22430 25.943 212 139 4 39 233 42 252 8.42e-14 71.6
MS.gene070869.t1 AT1G22440 27.273 187 118 4 39 208 40 225 1.24e-13 72.0
MS.gene070869.t1 AT4G22110 25.667 300 186 11 39 307 43 336 2.40e-13 71.2
MS.gene070869.t1 AT4G22110 25.667 300 186 11 39 307 43 336 2.40e-13 71.2
MS.gene070869.t1 AT1G64710 25.000 300 195 9 39 310 13 310 2.39e-12 67.8
MS.gene070869.t1 AT1G64710 25.000 300 195 9 39 310 36 333 2.63e-12 67.8
MS.gene070869.t1 AT4G22110 25.581 215 140 5 39 235 43 255 7.81e-12 65.5

Find 95 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCCGTCTTTCCTTTAATTT+TGG 0.091002 5.4:+21333672 MS.gene070869:CDS
ACCAATTGTAGTAGTGTATT+TGG 0.169399 5.4:-21333074 None:intergenic
TTGTTTGGATGTTCAAATTC+AGG 0.242919 5.4:-21332507 None:intergenic
GCTAGCTTACCCAAAATTAA+AGG 0.255595 5.4:-21333682 None:intergenic
GTGAAATTTGCCAAAGCTTT+TGG 0.281096 5.4:+21333319 MS.gene070869:CDS
CCAATTGTAGTAGTGTATTT+GGG 0.295426 5.4:-21333073 None:intergenic
ATCAACCTATCCACTAGTTC+CGG 0.329909 5.4:+21332819 MS.gene070869:CDS
CATCCTATCTTGCCACTACT+TGG 0.340131 5.4:+21333589 MS.gene070869:CDS
GCCCAACCAAAGGCCTTGTT+TGG 0.342284 5.4:-21332522 None:intergenic
GCTGCACCTCTCCTTTGTGC+TGG 0.348614 5.4:+21333202 MS.gene070869:CDS
GTTGTTGGTCTTGGTGGGCT+TGG 0.363937 5.4:+21333286 MS.gene070869:CDS
CCCAAATACACTACTACAAT+TGG 0.369731 5.4:+21333073 MS.gene070869:CDS
GTCAAAACTTTCAGAGAAAC+AGG 0.379942 5.4:+21332715 MS.gene070869:intron
GACACGAAAATTTAAGAACT+TGG 0.385264 5.4:-21334039 None:intergenic
GATATTTGACATCTGCTTTG+AGG 0.389547 5.4:-21333984 None:intergenic
CATATCGGTGTTGTTGGTCT+TGG 0.391909 5.4:+21333277 MS.gene070869:CDS
GATGCTTCCAATTCCACTTC+CGG 0.392808 5.4:-21333860 None:intergenic
CTGGTGGCCGGAAGTGGAAT+TGG 0.398456 5.4:+21333853 MS.gene070869:CDS
CACATTAGCACCAAAAGCTT+TGG 0.409643 5.4:-21333329 None:intergenic
TCTTGGTGGGCTTGGTCACA+TGG 0.411120 5.4:+21333294 MS.gene070869:CDS
TACAGTCCTCTTAGATACTT+TGG 0.411424 5.4:+21333235 MS.gene070869:CDS
GAACATCCAAACAAGGCCTT+TGG 0.412216 5.4:+21332516 MS.gene070869:CDS
ATCGGTGTTGTTGGTCTTGG+TGG 0.419928 5.4:+21333280 MS.gene070869:CDS
CTGCACCTCTCCTTTGTGCT+GGG 0.424848 5.4:+21333203 MS.gene070869:CDS
CGGAGGTTATTCTGACTCAT+TGG 0.425148 5.4:+21333126 MS.gene070869:CDS
ATAGTCTGCACTAGGTACTT+TGG 0.427527 5.4:+21333537 MS.gene070869:intron
TCAACCTATCCACTAGTTCC+GGG 0.430778 5.4:+21332820 MS.gene070869:CDS
TCCAAACAAGGCCTTTGGTT+GGG 0.431952 5.4:+21332521 MS.gene070869:CDS
GGTCTTCATATCGGTGTTGT+TGG 0.432442 5.4:+21333271 MS.gene070869:CDS
CCCAAAATTAAAGGAAAGAC+GGG 0.434247 5.4:-21333673 None:intergenic
CCCCTTTCAACTTCTCAAGA+AGG 0.434257 5.4:+21332571 MS.gene070869:CDS
AATCCAAGTAGTGGCAAGAT+AGG 0.440808 5.4:-21333592 None:intergenic
AGAAAGCTGGTGGCCGGAAG+TGG 0.451930 5.4:+21333847 MS.gene070869:CDS
GACAAACCTGGTCTTCATAT+CGG 0.456603 5.4:+21333262 MS.gene070869:CDS
GGGATCTCCAGAGGCTTGTC+AGG 0.464857 5.4:-21333652 None:intergenic
GCTTCAAGTGGTAAGCCATC+AGG 0.468059 5.4:-21333178 None:intergenic
GATAGGATGATTTGCTGAAA+CGG 0.468221 5.4:-21333575 None:intergenic
TTATTCTGACTCATTGGTTG+CGG 0.471466 5.4:+21333132 MS.gene070869:CDS
AACCTTCTTGAGAAGTTGAA+AGG 0.479627 5.4:-21332573 None:intergenic
ACCTTCTTGAGAAGTTGAAA+GGG 0.480830 5.4:-21332572 None:intergenic
TACATCTGCTAGCAAAGAAA+AGG 0.482166 5.4:+21333363 MS.gene070869:CDS
TATCGATTTGTGCTCGACAT+TGG 0.486206 5.4:+21334003 MS.gene070869:CDS
AAATACAGGCATGAGATTGT+TGG 0.500255 5.4:+21332926 MS.gene070869:intron
TGGTGTACCTGACAAGCCTC+TGG 0.501156 5.4:+21333645 MS.gene070869:CDS
AAAGTAGAAAAGTTCAATGT+TGG 0.511069 5.4:+21332971 MS.gene070869:CDS
CTGCATGGCGAAGAACGAAT+GGG 0.515871 5.4:+21332792 MS.gene070869:CDS
TCGAGTCCAAAGTATCTAAG+AGG 0.516519 5.4:-21333241 None:intergenic
GCAGGGAGAAAGCTGGTGGC+CGG 0.518096 5.4:+21333841 MS.gene070869:intron
ATCCAAACAAGGCCTTTGGT+TGG 0.532747 5.4:+21332520 MS.gene070869:CDS
TCTGCATGGCGAAGAACGAA+TGG 0.536596 5.4:+21332791 MS.gene070869:CDS
TCGGTGTTGTTGGTCTTGGT+GGG 0.546049 5.4:+21333281 MS.gene070869:CDS
GTTGGTATAGTGACAAAGGT+GGG 0.549964 5.4:+21332944 MS.gene070869:CDS
TGGGCAGCTAGAGACACATC+TGG 0.550336 5.4:+21332540 MS.gene070869:CDS
ACAGGAACGACTTCAATGTC+AGG 0.550360 5.4:-21333931 None:intergenic
CTTGGATTACTGAAGTCTCA+TGG 0.554281 5.4:+21333607 MS.gene070869:CDS
ACCCAAAATTAAAGGAAAGA+CGG 0.568562 5.4:-21333674 None:intergenic
TACATGGTTCACATTCCTGA+TGG 0.574098 5.4:+21333163 MS.gene070869:CDS
GTCTCTAGCTGCCCAACCAA+AGG 0.577379 5.4:-21332532 None:intergenic
TACTTTGGACTCGACAAACC+TGG 0.577584 5.4:+21333250 MS.gene070869:CDS
ATTGGTGCCAAGCATCGTGA+TGG 0.582249 5.4:+21333091 MS.gene070869:CDS
GTTGGAGACAAAGTAGGTGT+TGG 0.583429 5.4:+21332989 MS.gene070869:CDS
AAGTGGAATTGGAAGCATCA+AGG 0.586098 5.4:+21333864 MS.gene070869:CDS
CATGGAAAACTTGTAATGGT+TGG 0.586324 5.4:+21333625 MS.gene070869:CDS
GATGGTTCCATCACGATGCT+TGG 0.587249 5.4:-21333098 None:intergenic
TTCAATGTTGGAGACAAAGT+AGG 0.588338 5.4:+21332983 MS.gene070869:CDS
GTCTCATGGAAAACTTGTAA+TGG 0.588488 5.4:+21333621 MS.gene070869:CDS
AAGGAAGCAAAAGAACACTT+AGG 0.590978 5.4:+21333382 MS.gene070869:CDS
ATTATCGGCAGGGAGAAAGC+TGG 0.593426 5.4:+21333834 MS.gene070869:intron
AGAACATACCCCGGAACTAG+TGG 0.597931 5.4:-21332829 None:intergenic
CGTGATGGAACCATCACATA+CGG 0.603134 5.4:+21333106 MS.gene070869:CDS
GACTTCAGTAATCCAAGTAG+TGG 0.605130 5.4:-21333601 None:intergenic
AGAATAACCTCCGTATGTGA+TGG 0.606221 5.4:-21333116 None:intergenic
TGTTGGTATAGTGACAAAGG+TGG 0.606925 5.4:+21332943 MS.gene070869:CDS
CAATTGTAGTAGTGTATTTG+GGG 0.619152 5.4:-21333072 None:intergenic
TGACATTGAAGTCGTTCCTG+TGG 0.621293 5.4:+21333933 MS.gene070869:CDS
TCGTGACCAAGATAAAATGC+AGG 0.622175 5.4:+21333426 MS.gene070869:CDS
CCTTCTTGAGAAGTTGAAAG+GGG 0.624053 5.4:-21332571 None:intergenic
GTGATCCCAGCACAAAGGAG+AGG 0.625373 5.4:-21333208 None:intergenic
GCATTCAAAATATTGTATTG+TGG 0.625490 5.4:+21332751 MS.gene070869:CDS
TCTGCACTAGGTACTTTGGA+TGG 0.633602 5.4:+21333541 MS.gene070869:CDS
ATACCCCGGAACTAGTGGAT+AGG 0.638219 5.4:-21332824 None:intergenic
AGAGGTGCAGCAGCTTCAAG+TGG 0.642094 5.4:-21333190 None:intergenic
ATGTCACTCAGATGTCTGCA+TGG 0.642700 5.4:+21332777 MS.gene070869:CDS
GGTTGCGGATGAGAACTACA+TGG 0.644238 5.4:+21333147 MS.gene070869:CDS
GCTGTGTTAACATAATCCAC+AGG 0.647825 5.4:-21333949 None:intergenic
GGAAAGACGGGGATCTCCAG+AGG 0.652974 5.4:-21333661 None:intergenic
TGCATGGCGAAGAACGAATG+GGG 0.656200 5.4:+21332793 MS.gene070869:CDS
GATTGTTGGTATAGTGACAA+AGG 0.656994 5.4:+21332940 MS.gene070869:CDS
CAACCTATCCACTAGTTCCG+GGG 0.659512 5.4:+21332821 MS.gene070869:CDS
ATCGGCAGGGAGAAAGCTGG+TGG 0.663327 5.4:+21333837 MS.gene070869:intron
ACAACACCGATATGAAGACC+AGG 0.677179 5.4:-21333268 None:intergenic
CCAAAATTAAAGGAAAGACG+GGG 0.687666 5.4:-21333672 None:intergenic
TGAATTTGAACATCCAAACA+AGG 0.697834 5.4:+21332509 MS.gene070869:CDS
ACACAGTGATCCCAGCACAA+AGG 0.705727 5.4:-21333213 None:intergenic
GATGGAACCATCACATACGG+AGG 0.744602 5.4:+21333109 MS.gene070869:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATTTAAATAAGAAATAC+AGG + chr5.4:21332912-21332931 MS.gene070869:intron 10.0%
!! TAGTTCAATATTCTATTATA+CGG + chr5.4:21333460-21333479 MS.gene070869:intron 15.0%
!! ATTGAAAAAAAAAAGTGAAG+TGG - chr5.4:21332609-21332628 None:intergenic 20.0%
!! CAAATCAAAATAGTAGAAAA+TGG - chr5.4:21332889-21332908 None:intergenic 20.0%
!! TTAATCATATAAGTGATCTA+TGG - chr5.4:21333727-21333746 None:intergenic 20.0%
!! TTTATGATGCTAACAATTAT+CGG + chr5.4:21333819-21333838 MS.gene070869:intron 20.0%
!!! TTAAGAGTAAAATTGTTGTT+AGG + chr5.4:21332667-21332686 MS.gene070869:intron 20.0%
! AAAGTAGAAAAGTTCAATGT+TGG + chr5.4:21332971-21332990 MS.gene070869:CDS 25.0%
! AATTTAATTAGAACATACCC+CGG - chr5.4:21332841-21332860 None:intergenic 25.0%
! GCATTCAAAATATTGTATTG+TGG + chr5.4:21332751-21332770 MS.gene070869:CDS 25.0%
! TAATTAGTTCACGAATTGAT+TGG - chr5.4:21333504-21333523 None:intergenic 25.0%
ACCAATTGTAGTAGTGTATT+TGG - chr5.4:21333077-21333096 None:intergenic 30.0%
ACCCAAAATTAAAGGAAAGA+CGG - chr5.4:21333677-21333696 None:intergenic 30.0%
CAATTGTAGTAGTGTATTTG+GGG - chr5.4:21333075-21333094 None:intergenic 30.0%
CCAATTGTAGTAGTGTATTT+GGG - chr5.4:21333076-21333095 None:intergenic 30.0%
TGAATTTGAACATCCAAACA+AGG + chr5.4:21332509-21332528 MS.gene070869:CDS 30.0%
TTGTTTGGATGTTCAAATTC+AGG - chr5.4:21332510-21332529 None:intergenic 30.0%
! TTTGTACCTGCATTTTATCT+TGG - chr5.4:21333435-21333454 None:intergenic 30.0%
!! ATGTTTTTATAGTCTGCACT+AGG + chr5.4:21333529-21333548 MS.gene070869:intron 30.0%
!! TGAAAGTTTTGACACATCAA+AGG - chr5.4:21332708-21332727 None:intergenic 30.0%
AAATACAGGCATGAGATTGT+TGG + chr5.4:21332926-21332945 MS.gene070869:intron 35.0%
AACCTTCTTGAGAAGTTGAA+AGG - chr5.4:21332576-21332595 None:intergenic 35.0%
AAGGAAGCAAAAGAACACTT+AGG + chr5.4:21333382-21333401 MS.gene070869:CDS 35.0%
ACCTTCTTGAGAAGTTGAAA+GGG - chr5.4:21332575-21332594 None:intergenic 35.0%
AGAAACAGGTGAAAAAGATG+TGG + chr5.4:21332729-21332748 MS.gene070869:CDS 35.0%
CATGGAAAACTTGTAATGGT+TGG + chr5.4:21333625-21333644 MS.gene070869:CDS 35.0%
CCAAAATTAAAGGAAAGACG+GGG - chr5.4:21333675-21333694 None:intergenic 35.0%
CCCAAAATTAAAGGAAAGAC+GGG - chr5.4:21333676-21333695 None:intergenic 35.0%
CCCAAATACACTACTACAAT+TGG + chr5.4:21333073-21333092 MS.gene070869:CDS 35.0%
GATATTTGACATCTGCTTTG+AGG - chr5.4:21333987-21334006 None:intergenic 35.0%
GATTGTTGGTATAGTGACAA+AGG + chr5.4:21332940-21332959 MS.gene070869:CDS 35.0%
GTCAAAACTTTCAGAGAAAC+AGG + chr5.4:21332715-21332734 MS.gene070869:intron 35.0%
GTCTCATGGAAAACTTGTAA+TGG + chr5.4:21333621-21333640 MS.gene070869:CDS 35.0%
TACAGTCCTCTTAGATACTT+TGG + chr5.4:21333235-21333254 MS.gene070869:CDS 35.0%
TACATCTGCTAGCAAAGAAA+AGG + chr5.4:21333363-21333382 MS.gene070869:CDS 35.0%
TTCAATGTTGGAGACAAAGT+AGG + chr5.4:21332983-21333002 MS.gene070869:CDS 35.0%
! GCTAGCTTACCCAAAATTAA+AGG - chr5.4:21333685-21333704 None:intergenic 35.0%
! GTGAAATTTGCCAAAGCTTT+TGG + chr5.4:21333319-21333338 MS.gene070869:CDS 35.0%
! TTATTCTGACTCATTGGTTG+CGG + chr5.4:21333132-21333151 MS.gene070869:CDS 35.0%
!! CCCGTCTTTCCTTTAATTTT+GGG + chr5.4:21333673-21333692 MS.gene070869:CDS 35.0%
!! GATAGGATGATTTGCTGAAA+CGG - chr5.4:21333578-21333597 None:intergenic 35.0%
AATCCAAGTAGTGGCAAGAT+AGG - chr5.4:21333595-21333614 None:intergenic 40.0%
AGAATAACCTCCGTATGTGA+TGG - chr5.4:21333119-21333138 None:intergenic 40.0%
ATAGTCTGCACTAGGTACTT+TGG + chr5.4:21333537-21333556 MS.gene070869:intron 40.0%
ATCAACCTATCCACTAGTTC+CGG + chr5.4:21332819-21332838 MS.gene070869:CDS 40.0%
CACATTAGCACCAAAAGCTT+TGG - chr5.4:21333332-21333351 None:intergenic 40.0%
CCTTCTTGAGAAGTTGAAAG+GGG - chr5.4:21332574-21332593 None:intergenic 40.0%
CTTGGATTACTGAAGTCTCA+TGG + chr5.4:21333607-21333626 MS.gene070869:CDS 40.0%
GACTTCAGTAATCCAAGTAG+TGG - chr5.4:21333604-21333623 None:intergenic 40.0%
GATGCTAACAATTATCGGCA+GGG + chr5.4:21333824-21333843 MS.gene070869:intron 40.0%
GCTGTGTTAACATAATCCAC+AGG - chr5.4:21333952-21333971 None:intergenic 40.0%
GTTGGTATAGTGACAAAGGT+GGG + chr5.4:21332944-21332963 MS.gene070869:CDS 40.0%
TACATGGTTCACATTCCTGA+TGG + chr5.4:21333163-21333182 MS.gene070869:CDS 40.0%
TCGTGACCAAGATAAAATGC+AGG + chr5.4:21333426-21333445 MS.gene070869:CDS 40.0%
TGATGCTAACAATTATCGGC+AGG + chr5.4:21333823-21333842 MS.gene070869:intron 40.0%
TGTTGGTATAGTGACAAAGG+TGG + chr5.4:21332943-21332962 MS.gene070869:CDS 40.0%
! GACAAACCTGGTCTTCATAT+CGG + chr5.4:21333262-21333281 MS.gene070869:CDS 40.0%
! TCGAGTCCAAAGTATCTAAG+AGG - chr5.4:21333244-21333263 None:intergenic 40.0%
!! AAGTGGAATTGGAAGCATCA+AGG + chr5.4:21333864-21333883 MS.gene070869:CDS 40.0%
!! CCCCGTCTTTCCTTTAATTT+TGG + chr5.4:21333672-21333691 MS.gene070869:CDS 40.0%
!! TATCGATTTGTGCTCGACAT+TGG + chr5.4:21334003-21334022 MS.gene070869:CDS 40.0%
ACAACACCGATATGAAGACC+AGG - chr5.4:21333271-21333290 None:intergenic 45.0%
ACAGGAACGACTTCAATGTC+AGG - chr5.4:21333934-21333953 None:intergenic 45.0%
ATCCAAACAAGGCCTTTGGT+TGG + chr5.4:21332520-21332539 MS.gene070869:CDS 45.0%
ATGTCACTCAGATGTCTGCA+TGG + chr5.4:21332777-21332796 MS.gene070869:CDS 45.0%
CATCCTATCTTGCCACTACT+TGG + chr5.4:21333589-21333608 MS.gene070869:CDS 45.0%
CCCCTTTCAACTTCTCAAGA+AGG + chr5.4:21332571-21332590 MS.gene070869:CDS 45.0%
CGGAGGTTATTCTGACTCAT+TGG + chr5.4:21333126-21333145 MS.gene070869:CDS 45.0%
GAACATCCAAACAAGGCCTT+TGG + chr5.4:21332516-21332535 MS.gene070869:CDS 45.0%
GATGCTTCCAATTCCACTTC+CGG - chr5.4:21333863-21333882 None:intergenic 45.0%
GTTGGAGACAAAGTAGGTGT+TGG + chr5.4:21332989-21333008 MS.gene070869:CDS 45.0%
TACTTTGGACTCGACAAACC+TGG + chr5.4:21333250-21333269 MS.gene070869:CDS 45.0%
TCAACCTATCCACTAGTTCC+GGG + chr5.4:21332820-21332839 MS.gene070869:CDS 45.0%
TCCAAACAAGGCCTTTGGTT+GGG + chr5.4:21332521-21332540 MS.gene070869:CDS 45.0%
TCTGCACTAGGTACTTTGGA+TGG + chr5.4:21333541-21333560 MS.gene070869:CDS 45.0%
TGACATTGAAGTCGTTCCTG+TGG + chr5.4:21333933-21333952 MS.gene070869:CDS 45.0%
! CGTGATGGAACCATCACATA+CGG + chr5.4:21333106-21333125 MS.gene070869:CDS 45.0%
!! CATATCGGTGTTGTTGGTCT+TGG + chr5.4:21333277-21333296 MS.gene070869:CDS 45.0%
!! GGTCTTCATATCGGTGTTGT+TGG + chr5.4:21333271-21333290 MS.gene070869:CDS 45.0%
ACACAGTGATCCCAGCACAA+AGG - chr5.4:21333216-21333235 None:intergenic 50.0%
AGAACATACCCCGGAACTAG+TGG - chr5.4:21332832-21332851 None:intergenic 50.0%
ATTATCGGCAGGGAGAAAGC+TGG + chr5.4:21333834-21333853 MS.gene070869:intron 50.0%
CAACCTATCCACTAGTTCCG+GGG + chr5.4:21332821-21332840 MS.gene070869:CDS 50.0%
CTGCATGGCGAAGAACGAAT+GGG + chr5.4:21332792-21332811 MS.gene070869:CDS 50.0%
GATGGAACCATCACATACGG+AGG + chr5.4:21333109-21333128 MS.gene070869:CDS 50.0%
GATGGTTCCATCACGATGCT+TGG - chr5.4:21333101-21333120 None:intergenic 50.0%
GCTTCAAGTGGTAAGCCATC+AGG - chr5.4:21333181-21333200 None:intergenic 50.0%
GGTTGCGGATGAGAACTACA+TGG + chr5.4:21333147-21333166 MS.gene070869:CDS 50.0%
TCTGCATGGCGAAGAACGAA+TGG + chr5.4:21332791-21332810 MS.gene070869:CDS 50.0%
TGCATGGCGAAGAACGAATG+GGG + chr5.4:21332793-21332812 MS.gene070869:CDS 50.0%
! ATACCCCGGAACTAGTGGAT+AGG - chr5.4:21332827-21332846 None:intergenic 50.0%
!! ATCGGTGTTGTTGGTCTTGG+TGG + chr5.4:21333280-21333299 MS.gene070869:CDS 50.0%
!! ATTGGTGCCAAGCATCGTGA+TGG + chr5.4:21333091-21333110 MS.gene070869:CDS 50.0%
!! TCGGTGTTGTTGGTCTTGGT+GGG + chr5.4:21333281-21333300 MS.gene070869:CDS 50.0%
CTGCACCTCTCCTTTGTGCT+GGG + chr5.4:21333203-21333222 MS.gene070869:CDS 55.0%
GTCTCTAGCTGCCCAACCAA+AGG - chr5.4:21332535-21332554 None:intergenic 55.0%
GTGATCCCAGCACAAAGGAG+AGG - chr5.4:21333211-21333230 None:intergenic 55.0%
TGGGCAGCTAGAGACACATC+TGG + chr5.4:21332540-21332559 MS.gene070869:CDS 55.0%
TGGTGTACCTGACAAGCCTC+TGG + chr5.4:21333645-21333664 MS.gene070869:CDS 55.0%
! AGAGGTGCAGCAGCTTCAAG+TGG - chr5.4:21333193-21333212 None:intergenic 55.0%
!! GCCCAACCAAAGGCCTTGTT+TGG - chr5.4:21332525-21332544 None:intergenic 55.0%
!! GTTGTTGGTCTTGGTGGGCT+TGG + chr5.4:21333286-21333305 MS.gene070869:CDS 55.0%
!! TCTTGGTGGGCTTGGTCACA+TGG + chr5.4:21333294-21333313 MS.gene070869:CDS 55.0%
AGAAAGCTGGTGGCCGGAAG+TGG + chr5.4:21333847-21333866 MS.gene070869:CDS 60.0%
ATCGGCAGGGAGAAAGCTGG+TGG + chr5.4:21333837-21333856 MS.gene070869:intron 60.0%
CTGGTGGCCGGAAGTGGAAT+TGG + chr5.4:21333853-21333872 MS.gene070869:CDS 60.0%
GCTGCACCTCTCCTTTGTGC+TGG + chr5.4:21333202-21333221 MS.gene070869:CDS 60.0%
GGAAAGACGGGGATCTCCAG+AGG - chr5.4:21333664-21333683 None:intergenic 60.0%
GGGATCTCCAGAGGCTTGTC+AGG - chr5.4:21333655-21333674 None:intergenic 60.0%
GCAGGGAGAAAGCTGGTGGC+CGG + chr5.4:21333841-21333860 MS.gene070869:intron 65.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 21332495 21334050 21332495 ID=MS.gene070869
chr5.4 mRNA 21332495 21334050 21332495 ID=MS.gene070869.t1;Parent=MS.gene070869
chr5.4 exon 21332495 21332592 21332495 ID=MS.gene070869.t1.exon1;Parent=MS.gene070869.t1
chr5.4 CDS 21332495 21332592 21332495 ID=cds.MS.gene070869.t1;Parent=MS.gene070869.t1
chr5.4 exon 21332729 21332842 21332729 ID=MS.gene070869.t1.exon2;Parent=MS.gene070869.t1
chr5.4 CDS 21332729 21332842 21332729 ID=cds.MS.gene070869.t1;Parent=MS.gene070869.t1
chr5.4 exon 21332934 21333447 21332934 ID=MS.gene070869.t1.exon3;Parent=MS.gene070869.t1
chr5.4 CDS 21332934 21333447 21332934 ID=cds.MS.gene070869.t1;Parent=MS.gene070869.t1
chr5.4 exon 21333541 21333694 21333541 ID=MS.gene070869.t1.exon4;Parent=MS.gene070869.t1
chr5.4 CDS 21333541 21333694 21333541 ID=cds.MS.gene070869.t1;Parent=MS.gene070869.t1
chr5.4 exon 21333845 21334050 21333845 ID=MS.gene070869.t1.exon5;Parent=MS.gene070869.t1
chr5.4 CDS 21333845 21334050 21333845 ID=cds.MS.gene070869.t1;Parent=MS.gene070869.t1
Gene Sequence

>MS.gene070869

ATGGCAACACAACCTGAATTTGAACATCCAAACAAGGCCTTTGGTTGGGCAGCTAGAGACACATCTGGTGTTCTCTCCCCTTTCAACTTCTCAAGAAGGTTCATTCATACACCACTTCACTTTTTTTTTTCAATTAATTTATTTTTTACACTTTGATGCATCATATATAGTGTTAAGAGTAAAATTGTTGTTAGGAAAATATATAACTCACCTTTGATGTGTCAAAACTTTCAGAGAAACAGGTGAAAAAGATGTGGCATTCAAAATATTGTATTGTGGAATATGTCACTCAGATGTCTGCATGGCGAAGAACGAATGGGGCATATCAACCTATCCACTAGTTCCGGGGTATGTTCTAATTAAATTATCTTAGAAAAGTCTTAAAGTCTTACCATTTTCTACTATTTTGATTTGATTATAATTTAAATAAGAAATACAGGCATGAGATTGTTGGTATAGTGACAAAGGTGGGAAGCAAAGTAGAAAAGTTCAATGTTGGAGACAAAGTAGGTGTTGGCTACATAATTGATTCATGTCGATCGTGTCAAAATTGTGTTGACAATCTTGAAAATTACTGCCCCAAATACACTACTACAATTGGTGCCAAGCATCGTGATGGAACCATCACATACGGAGGTTATTCTGACTCATTGGTTGCGGATGAGAACTACATGGTTCACATTCCTGATGGCTTACCACTTGAAGCTGCTGCACCTCTCCTTTGTGCTGGGATCACTGTGTACAGTCCTCTTAGATACTTTGGACTCGACAAACCTGGTCTTCATATCGGTGTTGTTGGTCTTGGTGGGCTTGGTCACATGGCTGTGAAATTTGCCAAAGCTTTTGGTGCTAATGTGACTGTAATTAGTACATCTGCTAGCAAAGAAAAGGAAGCAAAAGAACACTTAGGAGCTGATTCATTTCTGATAAGTCGTGACCAAGATAAAATGCAGGTACAAAAACAATAGTTCAATATTCTATTATACGGAAATTTACAATGCTATTGCCAATCAATTCGTGAACTAATTATGTTAATGTTTTTATAGTCTGCACTAGGTACTTTGGATGGTATCATTGATACCGTTTCAGCAAATCATCCTATCTTGCCACTACTTGGATTACTGAAGTCTCATGGAAAACTTGTAATGGTTGGTGTACCTGACAAGCCTCTGGAGATCCCCGTCTTTCCTTTAATTTTGGGTAAGCTAGCATATATATGAACTAAGTTTCCATAGATCACTTATATGATTAAAAGCTATAAGTCATATATACATATATAGTATAATGCACATGAGAATATCAATTTAATTTATGAATAAGCATTTTTATGATGCTAACAATTATCGGCAGGGAGAAAGCTGGTGGCCGGAAGTGGAATTGGAAGCATCAAGGAAACTCAAGAGATGCTTGACTTTGCTGCTAAACACAATGTAAAACCTGACATTGAAGTCGTTCCTGTGGATTATGTTAACACAGCAATTGAACGACTCCTCAAAGCAGATGTCAAATATCGATTTGTGCTCGACATTGGAAACACTCTGAAACCAAGTTCTTAA
Protein sequence

>MS.gene070869.t1

MATQPEFEHPNKAFGWAARDTSGVLSPFNFSRRETGEKDVAFKILYCGICHSDVCMAKNEWGISTYPLVPGHEIVGIVTKVGSKVEKFNVGDKVGVGYIIDSCRSCQNCVDNLENYCPKYTTTIGAKHRDGTITYGGYSDSLVADENYMVHIPDGLPLEAAAPLLCAGITVYSPLRYFGLDKPGLHIGVVGLGGLGHMAVKFAKAFGANVTVISTSASKEKEAKEHLGADSFLISRDQDKMQSALGTLDGIIDTVSANHPILPLLGLLKSHGKLVMVGVPDKPLEIPVFPLILGRKLVAGSGIGSIKETQEMLDFAAKHNVKPDIEVVPVDYVNTAIERLLKADVKYRFVLDIGNTLKPSS