AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene068611

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068611.t1 MTR_5g031390 95.291 361 17 0 1 361 1 361 0.0 687
MS.gene068611.t1 MTR_5g031310 83.102 361 60 1 1 360 1 361 0.0 606
MS.gene068611.t1 MTR_5g031305 82.271 361 63 1 1 360 1 361 0.0 601
MS.gene068611.t1 MTR_5g031230 81.717 361 66 0 1 361 1 361 0.0 600
MS.gene068611.t1 MTR_5g031250 81.163 361 68 0 1 361 1 361 0.0 588
MS.gene068611.t1 MTR_4g115300 80.886 361 69 0 1 361 1 361 0.0 588
MS.gene068611.t1 MTR_5g031300 79.006 362 75 1 1 361 1 362 0.0 577
MS.gene068611.t1 MTR_5g031360 80.912 351 67 0 10 360 12 362 0.0 574
MS.gene068611.t1 MTR_5g031190 76.454 361 85 0 1 361 1 361 0.0 570
MS.gene068611.t1 MTR_5g031210 79.224 361 75 0 1 361 1 361 0.0 570
MS.gene068611.t1 MTR_5g031180 78.947 361 76 0 1 361 1 361 0.0 553
MS.gene068611.t1 MTR_8g068190 79.167 360 75 0 1 360 1 360 0.0 550
MS.gene068611.t1 MTR_8g092630 65.266 357 118 2 8 359 60 415 2.81e-157 448
MS.gene068611.t1 MTR_5g031430 60.290 345 137 0 14 358 14 358 2.22e-141 405
MS.gene068611.t1 MTR_1g025950 58.166 349 145 1 8 355 7 355 3.67e-135 390
MS.gene068611.t1 MTR_5g031250 80.498 241 47 0 1 241 1 241 3.73e-135 386
MS.gene068611.t1 MTR_1g107425 51.312 343 167 0 11 353 9 351 1.07e-117 345
MS.gene068611.t1 MTR_2g009810 52.450 347 161 3 14 358 62 406 6.77e-115 340
MS.gene068611.t1 MTR_1g107425 52.128 282 134 1 11 292 9 289 3.14e-92 278
MS.gene068611.t1 MTR_2g009820 51.064 235 106 2 130 358 23 254 1.25e-63 203
MS.gene068611.t1 MTR_5g031290 82.456 114 20 0 247 360 80 193 4.71e-61 194
MS.gene068611.t1 MTR_5g031290 85.000 80 11 1 1 79 1 80 7.47e-42 145
MS.gene068611.t1 MTR_8g095220 26.429 280 176 8 25 278 68 343 8.15e-20 90.5
MS.gene068611.t1 MTR_8g095220 30.275 218 126 8 25 221 68 280 1.02e-19 89.0
MS.gene068611.t1 MTR_3g089977 24.928 349 223 10 39 354 37 379 9.02e-19 87.0
MS.gene068611.t1 MTR_3g089940 25.074 339 216 9 39 344 37 370 1.57e-18 86.3
MS.gene068611.t1 MTR_3g089940 25.074 339 216 9 39 344 37 370 1.72e-18 86.3
MS.gene068611.t1 MTR_3g089970 24.779 339 217 9 39 344 38 371 5.89e-18 84.7
MS.gene068611.t1 MTR_6g465010 25.547 274 183 6 39 300 25 289 1.56e-17 83.2
MS.gene068611.t1 MTR_6g466320 25.547 274 183 6 39 300 41 305 1.66e-17 83.2
MS.gene068611.t1 MTR_6g465010 25.547 274 183 6 39 300 41 305 1.66e-17 83.2
MS.gene068611.t1 MTR_0001s0660 25.581 344 223 12 38 353 35 373 3.72e-16 79.7
MS.gene068611.t1 MTR_3g089940 25.333 300 194 7 39 312 37 332 6.13e-16 78.6
MS.gene068611.t1 MTR_3g089940 25.333 300 194 7 39 312 37 332 6.37e-16 78.6
MS.gene068611.t1 MTR_5g031380 57.407 54 18 1 80 128 19 72 1.02e-14 70.1
MS.gene068611.t1 MTR_7g068320 26.648 349 219 14 39 353 48 393 5.93e-14 72.8
MS.gene068611.t1 MTR_5g045710 23.411 299 200 8 38 312 35 328 7.58e-13 69.3
MS.gene068611.t1 MTR_7g099040 25.705 319 198 12 39 327 38 347 6.83e-12 66.6
MS.gene068611.t1 MTR_1g069430 26.023 342 212 14 1 312 1 331 7.94e-12 66.2
MS.gene068611.t1 MTR_3g079180 25.418 299 189 12 38 307 40 333 1.09e-11 65.9
MS.gene068611.t1 MTR_3g099460 26.688 311 194 11 39 319 37 343 4.42e-11 63.9
MS.gene068611.t1 MTR_3g099460 26.688 311 194 11 39 319 44 350 4.74e-11 63.9
MS.gene068611.t1 MTR_6g068930 25.798 376 229 16 11 351 13 373 6.97e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068611.t1 AT4G37980 70.857 350 102 0 11 360 7 356 1.66e-171 482
MS.gene068611.t1 AT4G37990 72.414 348 96 0 11 358 7 354 1.49e-170 479
MS.gene068611.t1 AT4G39330 62.465 357 134 0 1 357 1 357 7.61e-166 468
MS.gene068611.t1 AT4G37970 62.254 355 134 0 6 360 7 361 1.25e-159 452
MS.gene068611.t1 AT2G21890 61.671 347 132 1 12 357 6 352 1.47e-154 439
MS.gene068611.t1 AT2G21730 62.069 348 130 2 12 357 6 353 2.97e-154 439
MS.gene068611.t1 AT4G39330 62.046 303 115 0 1 303 1 303 1.26e-135 389
MS.gene068611.t1 AT4G37980 69.521 292 89 0 11 302 7 298 1.28e-132 381
MS.gene068611.t1 AT4G37970 59.932 292 117 0 6 297 7 298 2.57e-121 352
MS.gene068611.t1 AT1G72680 50.145 345 167 2 16 358 14 355 1.52e-112 332
MS.gene068611.t1 AT3G19450 52.023 346 166 0 11 356 9 354 4.15e-112 331
MS.gene068611.t1 AT4G34230 51.009 347 170 0 7 353 4 350 1.32e-101 304
MS.gene068611.t1 AT4G34230 50.720 347 171 0 7 353 4 350 1.36e-100 301
MS.gene068611.t1 AT4G37970 58.159 239 100 0 6 244 7 245 1.22e-93 280
MS.gene068611.t1 AT4G37970 58.650 237 98 0 6 242 7 243 1.86e-93 280
MS.gene068611.t1 AT5G63620 31.222 221 126 8 25 223 70 286 1.44e-23 99.8
MS.gene068611.t1 AT5G63620 28.169 284 166 11 25 278 70 345 2.07e-23 100
MS.gene068611.t1 AT5G63620 28.169 284 166 11 25 278 70 345 2.25e-23 100
MS.gene068611.t1 AT5G51970 26.545 275 179 8 39 300 43 307 6.81e-19 87.4
MS.gene068611.t1 AT5G51970 26.545 275 179 8 39 300 43 307 6.81e-19 87.4
MS.gene068611.t1 AT1G22430 27.586 319 195 13 39 327 42 354 3.16e-18 85.5
MS.gene068611.t1 AT1G22430 27.586 319 195 13 39 327 42 354 3.16e-18 85.5
MS.gene068611.t1 AT1G32780 27.198 364 214 16 39 358 37 393 2.13e-17 83.2
MS.gene068611.t1 AT1G22440 27.575 301 179 12 39 307 40 333 3.48e-17 82.4
MS.gene068611.t1 AT5G43940 25.143 350 217 12 39 352 36 376 1.89e-16 80.1
MS.gene068611.t1 AT5G43940 25.143 350 217 12 39 352 48 388 2.47e-16 80.1
MS.gene068611.t1 AT1G77120 24.069 349 226 11 39 354 36 378 2.50e-15 77.0
MS.gene068611.t1 AT1G22430 28.500 200 124 6 39 221 42 239 2.63e-14 73.2
MS.gene068611.t1 AT1G22430 28.500 200 124 6 39 221 42 239 2.63e-14 73.2
MS.gene068611.t1 AT1G22430 28.500 200 124 6 39 221 42 239 2.68e-14 73.2
MS.gene068611.t1 AT5G42250 23.569 297 196 9 39 307 45 338 3.17e-11 64.7

Find 87 sgRNAs with CRISPR-Local

Find 135 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATATTTCACATCTGCTTTA+AGG 0.136438 5.1:+21592402 None:intergenic
GCTGGTTCCATCACTATATT+TGG 0.170826 5.1:+21593386 None:intergenic
TATCGATTTGTGATTGATAT+TGG 0.198958 5.1:-21592383 MS.gene068611:CDS
GCCCAACCAAAGGCCTTCTT+TGG 0.253742 5.1:+21594713 None:intergenic
TATAGCACCTTCATCTGATT+TGG 0.268925 5.1:+21593051 None:intergenic
GTGAAATTTGCCAAAGCTTT+TGG 0.281096 5.1:-21593165 MS.gene068611:CDS
TCTTGGTGGACTTGGTCATA+TGG 0.309264 5.1:-21593190 MS.gene068611:CDS
CCGACCAGCACTTTGTGTTT+CGG 0.336603 5.1:-21593332 MS.gene068611:CDS
CACATTTGCACCAAAAGCTT+TGG 0.340626 5.1:+21593155 None:intergenic
AATTGAACCTTCTGGAGAAA+TGG 0.369501 5.1:+21594657 None:intergenic
GTTGTTGGTCTTGGTGGACT+TGG 0.377317 5.1:-21593198 MS.gene068611:CDS
ACCATCAGTGAGTGTCAATT+GGG 0.382344 5.1:+21593410 None:intergenic
ATAGGAATGATCTCAATATC+AGG 0.391852 5.1:+21592455 None:intergenic
CATCCTCTATTACCTCTGAT+TGG 0.394736 5.1:-21592766 MS.gene068611:CDS
GTTAGGCGATGTACTAATTA+CGG 0.396532 5.1:+21593131 None:intergenic
GAGCATCCAAAGAAGGCCTT+TGG 0.398624 5.1:-21594719 MS.gene068611:CDS
AACCATCAGTGAGTGTCAAT+TGG 0.401417 5.1:+21593409 None:intergenic
ATAGGTGTTGTTGGTCTTGG+TGG 0.407624 5.1:-21593204 MS.gene068611:CDS
AATATAGGTGTTGTTGGTCT+TGG 0.416186 5.1:-21593207 MS.gene068611:CDS
GCATCAAGAGGTAACTCATC+TGG 0.417564 5.1:+21593306 None:intergenic
TCCAAAGAAGGCCTTTGGTT+GGG 0.430490 5.1:-21594714 MS.gene068611:CDS
CCCCTTTCCATTTCTCCAGA+AGG 0.437633 5.1:-21594664 MS.gene068611:intron
TGGGCAGCTAGAGACTCTTC+TGG 0.441511 5.1:-21594695 MS.gene068611:CDS
ATATGCAGACATGAAATTGT+TGG 0.445405 5.1:-21593558 MS.gene068611:intron
TTCATAAGGCGAAGAATGAA+TGG 0.450733 5.1:-21593698 MS.gene068611:CDS
TGGAGGCTTCTCTGACTCAA+TGG 0.451601 5.1:-21593358 MS.gene068611:CDS
GGTATGAATATAGGTGTTGT+TGG 0.459605 5.1:-21593213 MS.gene068611:CDS
GTAGGGAGAAAGATAGTGGC+TGG 0.463044 5.1:-21592545 MS.gene068611:intron
GAATAATTTGTAGGCTGCAA+TGG 0.469877 5.1:-21592827 MS.gene068611:intron
CCTTCATCTGATTTGGATCA+CGG 0.476089 5.1:+21593058 None:intergenic
ACCTTCTGGAGAAATGGAAA+GGG 0.478815 5.1:+21594663 None:intergenic
GCTGTGTTGACGTAATCAAT+AGG 0.480301 5.1:+21592437 None:intergenic
GTAGGCTGCAATGGGTACTT+TGG 0.485806 5.1:-21592818 MS.gene068611:intron
ACGACCTATCCACTAGTGCC+TGG 0.488872 5.1:-21593669 MS.gene068611:CDS
TCATAAGGCGAAGAATGAAT+GGG 0.490720 5.1:-21593697 MS.gene068611:CDS
AATAATTTGTAGGCTGCAAT+GGG 0.491959 5.1:-21592826 MS.gene068611:intron
ATTCATTCTTCGCCTTATGA+AGG 0.497579 5.1:+21593700 None:intergenic
GCTGCTCCTCTCCTTTGTGC+TGG 0.498766 5.1:-21593282 MS.gene068611:CDS
ATGTCACTCGGACCTTCATA+AGG 0.505748 5.1:-21593712 MS.gene068611:CDS
ATAGTGGCTGGAAGTTTGAT+TGG 0.509085 5.1:-21592533 MS.gene068611:CDS
GACAAGCCTGGTATGAATAT+AGG 0.509901 5.1:-21593222 MS.gene068611:CDS
ATTGGTTTACTCAAGTCTCA+TGG 0.521108 5.1:-21592748 MS.gene068611:CDS
CAGAGTAGGTGTGGGATGCT+TGG 0.523124 5.1:-21593487 MS.gene068611:CDS
AGTGATGGAACCAGCACTCA+TGG 0.527999 5.1:-21593378 MS.gene068611:CDS
ATATATATACCTGCAAGTAA+AGG 0.531437 5.1:+21592673 None:intergenic
ATCCAAAGAAGGCCTTTGGT+TGG 0.536152 5.1:-21594715 MS.gene068611:CDS
TGAATTTGAGCATCCAAAGA+AGG 0.538083 5.1:-21594726 MS.gene068611:CDS
TACATCGCCTAACAAAGAAA+AGG 0.540053 5.1:-21593121 MS.gene068611:CDS
AAAGCTTACCCAGGCACTAG+TGG 0.540217 5.1:+21593660 None:intergenic
AAAGTGAGCAAGTTCAAAGT+TGG 0.541588 5.1:-21593513 MS.gene068611:CDS
AACCTTCTGGAGAAATGGAA+AGG 0.543081 5.1:+21594662 None:intergenic
AAACCAATCAGAGGTAATAG+AGG 0.546078 5.1:+21592763 None:intergenic
AGAGAAGCCTCCATGAGTGC+TGG 0.548669 5.1:+21593368 None:intergenic
ATTGGTAGGGAGAAAGATAG+TGG 0.553404 5.1:-21592549 MS.gene068611:intron
TTACCCAGGCACTAGTGGAT+AGG 0.556800 5.1:+21593665 None:intergenic
GTCTCATGGAAAGCTTGTAA+TGG 0.559201 5.1:-21592734 MS.gene068611:CDS
CCCCAATTGACACTCACTGA+TGG 0.559334 5.1:-21593411 MS.gene068611:CDS
TGGCTGGAAGTTTGATTGGT+GGG 0.560766 5.1:-21592529 MS.gene068611:CDS
GCTGCAATGGGTACTTTGGA+TGG 0.565027 5.1:-21592814 MS.gene068611:CDS
GAATCCGAAACACAAAGTGC+TGG 0.570488 5.1:+21593328 None:intergenic
CCGTGATCCAAATCAGATGA+AGG 0.574957 5.1:-21593058 MS.gene068611:intron
GTCTCTAGCTGCCCAACCAA+AGG 0.576314 5.1:+21594703 None:intergenic
AGTTGGAGACAGAGTAGGTG+TGG 0.577369 5.1:-21593496 MS.gene068611:CDS
GTTGGCATAGTTACAGAAGT+GGG 0.578876 5.1:-21593540 MS.gene068611:CDS
GTGGCTGGAAGTTTGATTGG+TGG 0.585158 5.1:-21592530 MS.gene068611:CDS
CGACCTATCCACTAGTGCCT+GGG 0.585971 5.1:-21593668 MS.gene068611:intron
TGTTGGCATAGTTACAGAAG+TGG 0.590305 5.1:-21593541 MS.gene068611:CDS
GATGGTTCCAAATATAGTGA+TGG 0.592641 5.1:-21593393 MS.gene068611:CDS
GCATTGAAAGTGTTGTATTG+TGG 0.600384 5.1:-21593738 MS.gene068611:CDS
CATGGAAAGCTTGTAATGGT+TGG 0.604281 5.1:-21592730 MS.gene068611:CDS
ATTTCACATCTGCTTTAAGG+AGG 0.611618 5.1:+21592405 None:intergenic
GTAATACCAGCACAAAGGAG+AGG 0.623289 5.1:+21593276 None:intergenic
TGATTACGTCAACACAGCGA+TGG 0.626468 5.1:-21592432 MS.gene068611:CDS
AGAGGAGCAGCAGCATCAAG+AGG 0.635270 5.1:+21593294 None:intergenic
TCGAGTTCAAAATGTCGAAG+AGG 0.636928 5.1:+21593243 None:intergenic
TTCAAAGTTGGAGACAGAGT+AGG 0.639117 5.1:-21593501 MS.gene068611:CDS
TATTATCGACACAGTTTCAG+CGG 0.643532 5.1:-21592791 MS.gene068611:CDS
GTTGGAGACAGAGTAGGTGT+GGG 0.643610 5.1:-21593495 MS.gene068611:CDS
CCGAAACACAAAGTGCTGGT+CGG 0.646762 5.1:+21593332 None:intergenic
ACAACACCTATATTCATACC+AGG 0.652836 5.1:+21593216 None:intergenic
CCTTCTGGAGAAATGGAAAG+GGG 0.654999 5.1:+21594664 None:intergenic
CCATCAGTGAGTGTCAATTG+GGG 0.658178 5.1:+21593411 None:intergenic
CATAAGGCGAAGAATGAATG+GGG 0.693942 5.1:-21593696 MS.gene068611:CDS
GACTTGAGTAAACCAATCAG+AGG 0.695064 5.1:+21592754 None:intergenic
ACACAGTAATACCAGCACAA+AGG 0.710961 5.1:+21593271 None:intergenic
GATGGAACCAGCACTCATGG+AGG 0.735076 5.1:-21593375 MS.gene068611:CDS
GTATTGTGGAATATGTCACT+CGG 0.739362 5.1:-21593724 MS.gene068611:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATATATTATGTAAAGT+AGG - chr5.1:21593124-21593143 MS.gene068611:CDS 10.0%
!! AATATATATTATGTAAAGTA+GGG - chr5.1:21593125-21593144 MS.gene068611:CDS 10.0%
!!! ATTTAATTTAAACATTTAGA+AGG + chr5.1:21594496-21594515 None:intergenic 10.0%
!!! TTTAATTTAAACATTTAGAA+GGG + chr5.1:21594495-21594514 None:intergenic 10.0%
!! AAATTCAATTTGAATCTAAT+TGG - chr5.1:21593508-21593527 MS.gene068611:CDS 15.0%
!! ATTAGTAAAATTTCTCAAAT+TGG - chr5.1:21594177-21594196 MS.gene068611:intron 15.0%
!! TATATCTTGATTTATATGTT+TGG + chr5.1:21593489-21593508 None:intergenic 15.0%
!!! ATCATTGTTATACATTTTTT+TGG + chr5.1:21593207-21593226 None:intergenic 15.0%
!! ATCATTTCATAATATATCGA+GGG + chr5.1:21592688-21592707 None:intergenic 20.0%
!! ATTGTAGTTCTCTTAAATTA+TGG + chr5.1:21592904-21592923 None:intergenic 20.0%
!! TAGTCAAAATTAGTAGAAAT+AGG + chr5.1:21592622-21592641 None:intergenic 20.0%
!! TATAGTTGCTTATTTAAAGA+CGG - chr5.1:21594099-21594118 MS.gene068611:intron 20.0%
!! TATCATTTCATAATATATCG+AGG + chr5.1:21592689-21592708 None:intergenic 20.0%
!! TATTATTTACACACTAAAGA+AGG + chr5.1:21593166-21593185 None:intergenic 20.0%
!! TCGATATATTATGAAATGAT+AGG - chr5.1:21592688-21592707 MS.gene068611:CDS 20.0%
!!! ATCAATCTAGAATAATTTGT+AGG - chr5.1:21594262-21594281 MS.gene068611:intron 20.0%
!!! GTATATTGTTTAGAATGAAA+AGG + chr5.1:21592482-21592501 None:intergenic 20.0%
!!! TATGTTTGGTTTTTTTGAAA+TGG + chr5.1:21593475-21593494 None:intergenic 20.0%
!!! TTAGTACGTTAAATTTTTCA+GGG - chr5.1:21593317-21593336 MS.gene068611:CDS 20.0%
!!! TTTAGTACGTTAAATTTTTC+AGG - chr5.1:21593316-21593335 MS.gene068611:CDS 20.0%
! AAGTATTTCACATTATTGGT+AGG - chr5.1:21594535-21594554 MS.gene068611:intron 25.0%
! AATGATGATATCAAACACTT+AGG + chr5.1:21592748-21592767 None:intergenic 25.0%
! AGTATTTCACATTATTGGTA+GGG - chr5.1:21594536-21594555 MS.gene068611:intron 25.0%
! ATATATATACCTGCAAGTAA+AGG + chr5.1:21594428-21594447 None:intergenic 25.0%
! CATAAAATAGAATGCTAAGA+AGG - chr5.1:21593233-21593252 MS.gene068611:CDS 25.0%
! CTAATTGTCAACTTCTAAAT+CGG + chr5.1:21594235-21594254 None:intergenic 25.0%
! GAAAAAGTGAGCAAATAAAA+TGG + chr5.1:21592563-21592582 None:intergenic 25.0%
! TCAAAAAAAGAAAGAACAGA+AGG - chr5.1:21593269-21593288 MS.gene068611:CDS 25.0%
! TCACAAGTATTTCACATTAT+TGG - chr5.1:21594531-21594550 MS.gene068611:intron 25.0%
! TGAAATACTTGTGAGTTTAA+AGG + chr5.1:21594525-21594544 None:intergenic 25.0%
!!! AATGCTTAACATTTGTTGTA+AGG - chr5.1:21592588-21592607 MS.gene068611:intron 25.0%
!!! TATCGATTTGTGATTGATAT+TGG - chr5.1:21594715-21594734 MS.gene068611:CDS 25.0%
AAATGATAGGATGCAAAAAG+TGG - chr5.1:21592701-21592720 MS.gene068611:CDS 30.0%
AATGAATGAATTGAACCTTC+TGG + chr5.1:21592452-21592471 None:intergenic 30.0%
AGTCACAATATTATTCAGCA+CGG - chr5.1:21594209-21594228 MS.gene068611:intron 30.0%
ATAGGAATGATCTCAATATC+AGG + chr5.1:21594646-21594665 None:intergenic 30.0%
ATATGCAGACATGAAATTGT+TGG - chr5.1:21593540-21593559 MS.gene068611:CDS 30.0%
ATTTCATAATATATCGAGGG+AGG + chr5.1:21592685-21592704 None:intergenic 30.0%
GATATTTCACATCTGCTTTA+AGG + chr5.1:21594699-21594718 None:intergenic 30.0%
TGATCAAACTTGACAGTTAT+CGG + chr5.1:21592801-21592820 None:intergenic 30.0%
TTGTTAACATTAGTGACTAG+TGG - chr5.1:21594154-21594173 MS.gene068611:intron 30.0%
TTTCATAATATATCGAGGGA+GGG + chr5.1:21592684-21592703 None:intergenic 30.0%
! AATAATTTGTAGGCTGCAAT+GGG - chr5.1:21594272-21594291 MS.gene068611:intron 30.0%
! ATTGCTTCCTTTTCTTTGTT+AGG + chr5.1:21593987-21594006 None:intergenic 30.0%
AAACCAATCAGAGGTAATAG+AGG + chr5.1:21594338-21594357 None:intergenic 35.0%
AAAGTGAGCAAGTTCAAAGT+TGG - chr5.1:21593585-21593604 MS.gene068611:intron 35.0%
ACAACACCTATATTCATACC+AGG + chr5.1:21593885-21593904 None:intergenic 35.0%
ACTTGACAGTTATCGGTAAA+TGG + chr5.1:21592794-21592813 None:intergenic 35.0%
AGGGCATATTTAGCATCATA+TGG + chr5.1:21594476-21594495 None:intergenic 35.0%
ATTCATTCTTCGCCTTATGA+AGG + chr5.1:21593401-21593420 None:intergenic 35.0%
GATGGTTCCAAATATAGTGA+TGG - chr5.1:21593705-21593724 MS.gene068611:CDS 35.0%
GTATTGTGGAATATGTCACT+CGG - chr5.1:21593374-21593393 MS.gene068611:CDS 35.0%
GTTAGGCGATGTACTAATTA+CGG + chr5.1:21593970-21593989 None:intergenic 35.0%
TAAAAGTAGAAAGCTTACCC+AGG + chr5.1:21593450-21593469 None:intergenic 35.0%
TACATCGCCTAACAAAGAAA+AGG - chr5.1:21593977-21593996 MS.gene068611:intron 35.0%
TATAGCACCTTCATCTGATT+TGG + chr5.1:21594050-21594069 None:intergenic 35.0%
TATTATCGACACAGTTTCAG+CGG - chr5.1:21594307-21594326 MS.gene068611:intron 35.0%
TCATAAGGCGAAGAATGAAT+GGG - chr5.1:21593401-21593420 MS.gene068611:CDS 35.0%
TGAATTTGAGCATCCAAAGA+AGG - chr5.1:21592372-21592391 MS.gene068611:CDS 35.0%
TTCATAAGGCGAAGAATGAA+TGG - chr5.1:21593400-21593419 MS.gene068611:CDS 35.0%
! AATTGAACCTTCTGGAGAAA+TGG + chr5.1:21592444-21592463 None:intergenic 35.0%
! ATTGGTTTACTCAAGTCTCA+TGG - chr5.1:21594350-21594369 MS.gene068611:intron 35.0%
! GAATAATTTGTAGGCTGCAA+TGG - chr5.1:21594271-21594290 MS.gene068611:intron 35.0%
! GGTATGAATATAGGTGTTGT+TGG - chr5.1:21593885-21593904 MS.gene068611:intron 35.0%
! GTGAAATTTGCCAAAGCTTT+TGG - chr5.1:21593933-21593952 MS.gene068611:intron 35.0%
!! AATATAGGTGTTGTTGGTCT+TGG - chr5.1:21593891-21593910 MS.gene068611:intron 35.0%
!! ATTTCACATCTGCTTTAAGG+AGG + chr5.1:21594696-21594715 None:intergenic 35.0%
!! GCATTGAAAGTGTTGTATTG+TGG - chr5.1:21593360-21593379 MS.gene068611:CDS 35.0%
AACCATCAGTGAGTGTCAAT+TGG + chr5.1:21593692-21593711 None:intergenic 40.0%
ACACAGTAATACCAGCACAA+AGG + chr5.1:21593830-21593849 None:intergenic 40.0%
ACCATCAGTGAGTGTCAATT+GGG + chr5.1:21593691-21593710 None:intergenic 40.0%
ATAGTGGCTGGAAGTTTGAT+TGG - chr5.1:21594565-21594584 MS.gene068611:intron 40.0%
ATTGGTAGGGAGAAAGATAG+TGG - chr5.1:21594549-21594568 MS.gene068611:intron 40.0%
CACATTTGCACCAAAAGCTT+TGG + chr5.1:21593946-21593965 None:intergenic 40.0%
CATAAGGCGAAGAATGAATG+GGG - chr5.1:21593402-21593421 MS.gene068611:CDS 40.0%
CATCCTCTATTACCTCTGAT+TGG - chr5.1:21594332-21594351 MS.gene068611:intron 40.0%
CATGGAAAGCTTGTAATGGT+TGG - chr5.1:21594368-21594387 MS.gene068611:intron 40.0%
CCTGCAAGTAAAGGAAAAAC+AGG + chr5.1:21594419-21594438 None:intergenic 40.0%
GACAAGCCTGGTATGAATAT+AGG - chr5.1:21593876-21593895 MS.gene068611:intron 40.0%
GACTTGAGTAAACCAATCAG+AGG + chr5.1:21594347-21594366 None:intergenic 40.0%
GTCTCATGGAAAGCTTGTAA+TGG - chr5.1:21594364-21594383 MS.gene068611:intron 40.0%
GTTGGCATAGTTACAGAAGT+GGG - chr5.1:21593558-21593577 MS.gene068611:intron 40.0%
TCGAGTTCAAAATGTCGAAG+AGG + chr5.1:21593858-21593877 None:intergenic 40.0%
TGATAGGATGCAAAAAGTGG+AGG - chr5.1:21592704-21592723 MS.gene068611:CDS 40.0%
TGTTGGCATAGTTACAGAAG+TGG - chr5.1:21593557-21593576 MS.gene068611:intron 40.0%
TTCAAAGTTGGAGACAGAGT+AGG - chr5.1:21593597-21593616 MS.gene068611:intron 40.0%
! AACCTTCTGGAGAAATGGAA+AGG + chr5.1:21592439-21592458 None:intergenic 40.0%
! ACCTTCTGGAGAAATGGAAA+GGG + chr5.1:21592438-21592457 None:intergenic 40.0%
! GCTGGTTCCATCACTATATT+TGG + chr5.1:21593715-21593734 None:intergenic 40.0%
! GCTGTGTTGACGTAATCAAT+AGG + chr5.1:21594664-21594683 None:intergenic 40.0%
!! CCTGTTTTTCCTTTACTTGC+AGG - chr5.1:21594416-21594435 MS.gene068611:intron 40.0%
!! CCTTCATCTGATTTGGATCA+CGG + chr5.1:21594043-21594062 None:intergenic 40.0%
ATCCAAAGAAGGCCTTTGGT+TGG - chr5.1:21592383-21592402 MS.gene068611:CDS 45.0%
ATGTCACTCGGACCTTCATA+AGG - chr5.1:21593386-21593405 MS.gene068611:CDS 45.0%
CCATCAGTGAGTGTCAATTG+GGG + chr5.1:21593690-21593709 None:intergenic 45.0%
CCGTGATCCAAATCAGATGA+AGG - chr5.1:21594040-21594059 MS.gene068611:intron 45.0%
GAATCCGAAACACAAAGTGC+TGG + chr5.1:21593773-21593792 None:intergenic 45.0%
GCATCAAGAGGTAACTCATC+TGG + chr5.1:21593795-21593814 None:intergenic 45.0%
GTAATACCAGCACAAAGGAG+AGG + chr5.1:21593825-21593844 None:intergenic 45.0%
TCCAAAGAAGGCCTTTGGTT+GGG - chr5.1:21592384-21592403 MS.gene068611:CDS 45.0%
TGATTACGTCAACACAGCGA+TGG - chr5.1:21594666-21594685 MS.gene068611:CDS 45.0%
TGGCTGGAAGTTTGATTGGT+GGG - chr5.1:21594569-21594588 MS.gene068611:intron 45.0%
! CATTTTGAACTCGACAAGCC+TGG - chr5.1:21593864-21593883 MS.gene068611:intron 45.0%
! CCTTCTGGAGAAATGGAAAG+GGG + chr5.1:21592437-21592456 None:intergenic 45.0%
! GGAAAAACAGGAAGCTCTAG+AGG + chr5.1:21594407-21594426 None:intergenic 45.0%
!! ATAGGTGTTGTTGGTCTTGG+TGG - chr5.1:21593894-21593913 MS.gene068611:intron 45.0%
!! TCTTGGTGGACTTGGTCATA+TGG - chr5.1:21593908-21593927 MS.gene068611:intron 45.0%
!! TTAAATTTAAAAAAACAATT+AGG + chr5.1:21592874-21592893 None:intergenic 5.0%
AAAGCTTACCCAGGCACTAG+TGG + chr5.1:21593441-21593460 None:intergenic 50.0%
AGTTGGAGACAGAGTAGGTG+TGG - chr5.1:21593602-21593621 MS.gene068611:intron 50.0%
CCCCAATTGACACTCACTGA+TGG - chr5.1:21593687-21593706 MS.gene068611:CDS 50.0%
CCCCTTTCCATTTCTCCAGA+AGG - chr5.1:21592434-21592453 MS.gene068611:CDS 50.0%
CCGACCAGCACTTTGTGTTT+CGG - chr5.1:21593766-21593785 MS.gene068611:intron 50.0%
GAGCATCCAAAGAAGGCCTT+TGG - chr5.1:21592379-21592398 MS.gene068611:CDS 50.0%
GCTGCAATGGGTACTTTGGA+TGG - chr5.1:21594284-21594303 MS.gene068611:intron 50.0%
GTAGGCTGCAATGGGTACTT+TGG - chr5.1:21594280-21594299 MS.gene068611:intron 50.0%
GTGGCTGGAAGTTTGATTGG+TGG - chr5.1:21594568-21594587 MS.gene068611:intron 50.0%
GTTGGAGACAGAGTAGGTGT+GGG - chr5.1:21593603-21593622 MS.gene068611:intron 50.0%
TGGAGGCTTCTCTGACTCAA+TGG - chr5.1:21593740-21593759 MS.gene068611:CDS 50.0%
! AGTGATGGAACCAGCACTCA+TGG - chr5.1:21593720-21593739 MS.gene068611:CDS 50.0%
! GTAGGGAGAAAGATAGTGGC+TGG - chr5.1:21594553-21594572 MS.gene068611:intron 50.0%
! TTACCCAGGCACTAGTGGAT+AGG + chr5.1:21593436-21593455 None:intergenic 50.0%
!! CCGAAACACAAAGTGCTGGT+CGG + chr5.1:21593769-21593788 None:intergenic 50.0%
!! GTTGTTGGTCTTGGTGGACT+TGG - chr5.1:21593900-21593919 MS.gene068611:intron 50.0%
!!! GGAAGCTCTAGAGGCTTTTC+AGG + chr5.1:21594398-21594417 None:intergenic 50.0%
ACGACCTATCCACTAGTGCC+TGG - chr5.1:21593429-21593448 MS.gene068611:CDS 55.0%
AGAGAAGCCTCCATGAGTGC+TGG + chr5.1:21593733-21593752 None:intergenic 55.0%
AGAGGAGCAGCAGCATCAAG+AGG + chr5.1:21593807-21593826 None:intergenic 55.0%
CAGAGTAGGTGTGGGATGCT+TGG - chr5.1:21593611-21593630 MS.gene068611:intron 55.0%
CGACCTATCCACTAGTGCCT+GGG - chr5.1:21593430-21593449 MS.gene068611:CDS 55.0%
GATGGAACCAGCACTCATGG+AGG - chr5.1:21593723-21593742 MS.gene068611:CDS 55.0%
GCCCAACCAAAGGCCTTCTT+TGG + chr5.1:21592388-21592407 None:intergenic 55.0%
GTCTCTAGCTGCCCAACCAA+AGG + chr5.1:21592398-21592417 None:intergenic 55.0%
TGGGCAGCTAGAGACTCTTC+TGG - chr5.1:21592403-21592422 MS.gene068611:CDS 55.0%
GCTGCTCCTCTCCTTTGTGC+TGG - chr5.1:21593816-21593835 MS.gene068611:intron 60.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 21592358 21594762 21592358 ID=MS.gene068611
chr5.1 mRNA 21592358 21594762 21592358 ID=MS.gene068611.t1;Parent=MS.gene068611
chr5.1 exon 21594665 21594762 21594665 ID=MS.gene068611.t1.exon1;Parent=MS.gene068611.t1
chr5.1 CDS 21594665 21594762 21594665 ID=cds.MS.gene068611.t1;Parent=MS.gene068611.t1
chr5.1 exon 21593669 21593782 21593669 ID=MS.gene068611.t1.exon2;Parent=MS.gene068611.t1
chr5.1 CDS 21593669 21593782 21593669 ID=cds.MS.gene068611.t1;Parent=MS.gene068611.t1
chr5.1 exon 21593059 21593572 21593059 ID=MS.gene068611.t1.exon3;Parent=MS.gene068611.t1
chr5.1 CDS 21593059 21593572 21593059 ID=cds.MS.gene068611.t1;Parent=MS.gene068611.t1
chr5.1 exon 21592683 21592836 21592683 ID=MS.gene068611.t1.exon4;Parent=MS.gene068611.t1
chr5.1 CDS 21592683 21592836 21592683 ID=cds.MS.gene068611.t1;Parent=MS.gene068611.t1
chr5.1 exon 21592358 21592563 21592358 ID=MS.gene068611.t1.exon5;Parent=MS.gene068611.t1
chr5.1 CDS 21592358 21592563 21592358 ID=cds.MS.gene068611.t1;Parent=MS.gene068611.t1
Gene Sequence

>MS.gene068611

ATGGCAACACAACATGAATTTGAGCATCCAAAGAAGGCCTTTGGTTGGGCAGCTAGAGACTCTTCTGGTGTTCTATCCCCTTTCCATTTCTCCAGAAGGTTCAATTCATTCATTATGATCACCTTTTCATTCTAAACAATATACATGTAGTATTCATTTTTTTTTGTTGACAAAATGCATGTAGTATTCATTATTTGTTGCACCATTTTATTTGCTCACTTTTTCTTTATAATGCTTAACATTTGTTGTAAGGTGTTATTTCCTATTTCTACTAATTTTGACTAAAATATAAATAACATCATGAATTTATTTACTTGATTTTTCCCTCCCTCGATATATTATGAAATGATAGGATGCAAAAAGTGGAGGATATGAATCATCTTATGTCCTAAGTGTTTGATATCATCATTATATAGAGATATGTTAACAATAACCATTTACCGATAACTGTCAAGTTTGATCACTTTTTTTCTTTCTTTTTTTTTTTTTTTACAAATTTAATTACATTTAAAACCTAATTGTTTTTTTAAATTTAATTTTTCACCATAATTTAAGAGAACTACAATGCTAACAATCAGTTATAGCTAAACATTTCTCTATACAAATTTACTCATAACAACACAACATGTTTTCTTTCTAAAAAAAATACTATGTTTTCAAATTCATATTTCAATTTAAAAACATATATTATGAATATAAATACTGAAATTTAGTTAGTCTACATAATATAGAAATTATAAAATTAAAATCGATGAAATAAATTGAAAAATATATATTATGTAAAGTAGGGAAAAAAAATAGACGTCCTTCTTTAGTGTGTAAATAATAATAGTAACAGTCTATCTACCAAAAAAATGTATAACAATGATAAAAATCATAAAATAGAATGCTAAGAAGGAAGTTTGATCTTGTCAAAAAAAGAAAGAACAGAAGGAAGTATGATCACAATTAAATCTAATTTAGTACGTTAAATTTTTCAGGGAAACATGTGAGAAAGACGTAGCATTGAAAGTGTTGTATTGTGGAATATGTCACTCGGACCTTCATAAGGCGAAGAATGAATGGGGCACTACGACCTATCCACTAGTGCCTGGGTAAGCTTTCTACTTTTATTCCATTTCAAAAAAACCAAACATATAAATCAAGATATAAATTCAATTTGAATCTAATTGGTGTAAAAATATATGCAGACATGAAATTGTTGGCATAGTTACAGAAGTGGGAAGCAAAGTGAGCAAGTTCAAAGTTGGAGACAGAGTAGGTGTGGGATGCTTGGTTGATTCTTGTCACTCATGTCAAAATTGTGTTGATAATCTTGAGAATTACTGTCCCCAATTGACACTCACTGATGGTTCCAAATATAGTGATGGAACCAGCACTCATGGAGGCTTCTCTGACTCAATGGTTTCCGACCAGCACTTTGTGTTTCGGATTCCAGATGAGTTACCTCTTGATGCTGCTGCTCCTCTCCTTTGTGCTGGTATTACTGTGTATAGTCCTCTTCGACATTTTGAACTCGACAAGCCTGGTATGAATATAGGTGTTGTTGGTCTTGGTGGACTTGGTCATATGGCTGTGAAATTTGCCAAAGCTTTTGGTGCAAATGTGACCGTAATTAGTACATCGCCTAACAAAGAAAAGGAAGCAATAGAACACTTGAGAGCTGACTCATTTCTAATAAGCCGTGATCCAAATCAGATGAAGGTGCTATAATTAATTTAATTCACTGTAAAAATTGTCGTATAGTTGCTTATTTAAAGACGGAAAATGTCAAATCGATCACTAAATTAATTTGATTGTTAACATTAGTGACTAGTGGATTAGTAAAATTTCTCAAATTGGTCTCTAATGAGTCACAATATTATTCAGCACGGCCGATTTAGAAGTTGACAATTAGTGAACTAATCAATCTAGAATAATTTGTAGGCTGCAATGGGTACTTTGGATGGTATTATCGACACAGTTTCAGCGGATCATCCTCTATTACCTCTGATTGGTTTACTCAAGTCTCATGGAAAGCTTGTAATGGTTGGTGCACCTGAAAAGCCTCTAGAGCTTCCTGTTTTTCCTTTACTTGCAGGTATATATATAAGCATTTGAAAAATATATATACTTCCATATGATGCTAAATATGCCCTTCTAAATGTTTAAATTAAATTATGAACCTTTAAACTCACAAGTATTTCACATTATTGGTAGGGAGAAAGATAGTGGCTGGAAGTTTGATTGGTGGGATCAAAGAGACTCAAGAAATGATTGATTTTGCAGCAGAACACAATGTAAAGCCTGATATTGAGATCATTCCTATTGATTACGTCAACACAGCGATGGAGCGCCTCCTTAAAGCAGATGTGAAATATCGATTTGTGATTGATATTGGAAACACATTAAAATCAAGCTCTTGA
Protein sequence

>MS.gene068611.t1

MATQHEFEHPKKAFGWAARDSSGVLSPFHFSRRETCEKDVALKVLYCGICHSDLHKAKNEWGTTTYPLVPGHEIVGIVTEVGSKVSKFKVGDRVGVGCLVDSCHSCQNCVDNLENYCPQLTLTDGSKYSDGTSTHGGFSDSMVSDQHFVFRIPDELPLDAAAPLLCAGITVYSPLRHFELDKPGMNIGVVGLGGLGHMAVKFAKAFGANVTVISTSPNKEKEAIEHLRADSFLISRDPNQMKAAMGTLDGIIDTVSADHPLLPLIGLLKSHGKLVMVGAPEKPLELPVFPLLAGRKIVAGSLIGGIKETQEMIDFAAEHNVKPDIEIIPIDYVNTAMERLLKADVKYRFVIDIGNTLKSSS