AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene027823

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027823.t1 MTR_5g031250 96.122 361 14 0 1 361 1 361 0.0 725
MS.gene027823.t1 MTR_5g031230 81.994 361 65 0 1 361 1 361 0.0 610
MS.gene027823.t1 MTR_4g115300 81.717 361 66 0 1 361 1 361 0.0 607
MS.gene027823.t1 MTR_5g031390 80.055 361 72 0 1 361 1 361 0.0 591
MS.gene027823.t1 MTR_5g031310 80.055 361 71 1 1 360 1 361 0.0 590
MS.gene027823.t1 MTR_5g031210 79.501 361 74 0 1 361 1 361 0.0 585
MS.gene027823.t1 MTR_5g031190 78.670 361 77 0 1 361 1 361 0.0 584
MS.gene027823.t1 MTR_5g031305 79.501 361 73 1 1 360 1 361 0.0 581
MS.gene027823.t1 MTR_5g031300 79.006 362 75 1 1 361 1 362 0.0 580
MS.gene027823.t1 MTR_5g031180 80.886 361 69 0 1 361 1 361 0.0 570
MS.gene027823.t1 MTR_5g031360 76.923 351 81 0 10 360 12 362 0.0 560
MS.gene027823.t1 MTR_8g068190 77.778 360 80 0 1 360 1 360 0.0 559
MS.gene027823.t1 MTR_5g031250 96.680 241 8 0 1 241 1 241 1.11e-177 493
MS.gene027823.t1 MTR_8g092630 62.817 355 126 2 8 357 60 413 2.02e-152 436
MS.gene027823.t1 MTR_5g031430 62.029 345 131 0 14 358 14 358 5.11e-150 427
MS.gene027823.t1 MTR_1g025950 57.880 349 146 1 8 355 7 355 7.37e-137 394
MS.gene027823.t1 MTR_1g107425 53.353 343 160 0 11 353 9 351 1.26e-126 368
MS.gene027823.t1 MTR_2g009810 52.312 346 163 2 14 358 62 406 6.75e-119 350
MS.gene027823.t1 MTR_1g107425 54.770 283 127 1 11 293 9 290 8.34e-102 303
MS.gene027823.t1 MTR_2g009820 47.674 258 109 4 116 358 8 254 1.28e-64 206
MS.gene027823.t1 MTR_5g031290 78.070 114 25 0 247 360 80 193 5.16e-58 187
MS.gene027823.t1 MTR_5g031290 86.250 80 10 1 1 79 1 80 4.51e-44 150
MS.gene027823.t1 MTR_8g095220 25.714 280 178 7 25 278 68 343 2.67e-22 97.8
MS.gene027823.t1 MTR_8g095220 27.273 242 151 6 25 245 68 305 3.07e-22 95.9
MS.gene027823.t1 MTR_6g465010 25.460 326 215 10 15 325 3 315 5.79e-19 87.0
MS.gene027823.t1 MTR_6g466320 25.294 340 225 11 1 325 6 331 6.21e-19 87.4
MS.gene027823.t1 MTR_6g465010 25.294 340 225 11 1 325 6 331 6.21e-19 87.4
MS.gene027823.t1 MTR_0001s0660 25.497 302 201 10 38 318 35 333 1.03e-15 78.6
MS.gene027823.t1 MTR_3g089977 22.350 349 232 11 39 354 37 379 1.75e-15 77.4
MS.gene027823.t1 MTR_3g089940 22.616 367 243 12 11 344 12 370 2.74e-15 76.6
MS.gene027823.t1 MTR_3g089940 22.419 339 225 10 39 344 37 370 3.42e-15 76.6
MS.gene027823.t1 MTR_3g089970 21.829 339 227 10 39 344 38 371 1.82e-13 71.2
MS.gene027823.t1 MTR_3g089940 22.150 307 209 8 39 319 37 339 3.85e-13 70.1
MS.gene027823.t1 MTR_3g089940 22.388 335 227 10 11 319 12 339 3.92e-13 70.1
MS.gene027823.t1 MTR_5g031380 66.667 39 13 0 80 118 19 57 6.91e-13 65.1
MS.gene027823.t1 MTR_1g069430 24.854 342 216 13 1 312 1 331 2.26e-12 68.2
MS.gene027823.t1 MTR_7g099040 26.332 319 194 13 39 324 38 348 6.12e-12 66.6
MS.gene027823.t1 MTR_6g068930 25.065 387 240 18 2 353 4 375 1.79e-11 65.1
MS.gene027823.t1 MTR_5g045710 22.896 297 204 7 38 312 35 328 4.54e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027823.t1 AT4G37990 72.571 350 96 0 11 360 7 356 8.30e-175 490
MS.gene027823.t1 AT4G37970 65.070 355 124 0 6 360 7 361 4.07e-170 478
MS.gene027823.t1 AT4G37980 68.286 350 111 0 11 360 7 356 8.15e-166 467
MS.gene027823.t1 AT4G39330 61.281 359 139 0 1 359 1 359 1.34e-165 467
MS.gene027823.t1 AT2G21890 60.969 351 136 1 12 361 6 356 3.78e-157 446
MS.gene027823.t1 AT2G21730 61.080 352 135 2 12 361 6 357 4.24e-156 443
MS.gene027823.t1 AT4G39330 60.133 301 120 0 1 301 1 301 1.78e-132 381
MS.gene027823.t1 AT4G37970 62.976 289 107 0 6 294 7 295 5.98e-130 374
MS.gene027823.t1 AT4G37980 68.310 284 90 0 11 294 7 290 2.48e-128 370
MS.gene027823.t1 AT1G72680 50.437 343 169 1 16 358 14 355 4.21e-115 338
MS.gene027823.t1 AT3G19450 53.179 346 162 0 11 356 9 354 3.31e-114 337
MS.gene027823.t1 AT4G34230 54.179 347 159 0 7 353 4 350 1.77e-110 327
MS.gene027823.t1 AT4G34230 53.890 347 160 0 7 353 4 350 1.67e-109 324
MS.gene027823.t1 AT4G37970 63.713 237 86 0 6 242 7 243 3.32e-105 310
MS.gene027823.t1 AT4G37970 63.713 237 86 0 6 242 7 243 3.83e-105 310
MS.gene027823.t1 AT5G63620 25.979 281 176 8 25 278 70 345 1.03e-22 99.0
MS.gene027823.t1 AT5G63620 25.979 281 176 8 25 278 70 345 1.11e-22 99.0
MS.gene027823.t1 AT5G63620 26.556 241 152 6 25 244 70 306 1.44e-22 97.1
MS.gene027823.t1 AT5G51970 25.163 306 203 9 35 325 39 333 2.39e-17 82.8
MS.gene027823.t1 AT5G51970 25.163 306 203 9 35 325 39 333 2.39e-17 82.8
MS.gene027823.t1 AT5G43940 25.501 349 217 12 39 352 36 376 1.59e-16 80.5
MS.gene027823.t1 AT5G43940 25.501 349 217 12 39 352 48 388 1.96e-16 80.5
MS.gene027823.t1 AT1G22430 24.375 320 202 12 39 324 42 355 3.41e-15 76.6
MS.gene027823.t1 AT1G22430 24.375 320 202 12 39 324 42 355 3.41e-15 76.6
MS.gene027823.t1 AT1G22430 26.000 200 129 5 39 221 42 239 1.07e-13 71.6
MS.gene027823.t1 AT1G32780 26.243 362 220 14 39 358 37 393 1.23e-13 72.0
MS.gene027823.t1 AT1G22430 26.000 200 129 5 39 221 42 239 1.35e-13 70.9
MS.gene027823.t1 AT1G22430 26.000 200 129 5 39 221 42 239 1.35e-13 70.9
MS.gene027823.t1 AT1G77120 20.930 387 264 11 1 354 1 378 1.90e-12 68.2

Find 88 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATAGTCCTCTTCGATATTT+TGG 0.153434 5.2:+21555526 MS.gene027823:CDS
CCCTCACATTCCTTTAATAA+TGG 0.196862 5.2:+21555954 MS.gene027823:CDS
TATCGATTTGTGATTGATAT+TGG 0.198958 5.2:+21556297 MS.gene027823:CDS
TTCTTTGGATGTTCAAATTC+AGG 0.206123 5.2:-21554780 None:intergenic
TATTGCTTCTTTCTCTTTGT+TGG 0.211892 5.2:-21555662 None:intergenic
CTATATGTTTGTGTTTGTTT+AGG 0.221743 5.2:-21555364 None:intergenic
AACAACTTACCCATTATTAA+AGG 0.228051 5.2:-21555964 None:intergenic
GCCCAACCAAATGCCTTCTT+TGG 0.260645 5.2:-21554795 None:intergenic
TATATGTTTGTGTTTGTTTA+GGG 0.265623 5.2:-21555363 None:intergenic
CCTCACATTCCTTTAATAAT+GGG 0.310674 5.2:+21555955 MS.gene027823:CDS
CCCCTTTCAACTTCTCTAGA+AGG 0.321261 5.2:+21554844 MS.gene027823:CDS
TCTTGGTGGACTTGGTCACT+TGG 0.333930 5.2:+21555585 MS.gene027823:CDS
ATTGCTTCTTTCTCTTTGTT+GGG 0.338643 5.2:-21555661 None:intergenic
GTGAAGTTTGCAAAAGCTTT+TGG 0.362966 5.2:+21555610 MS.gene027823:CDS
GAACATCCAAAGAAGGCATT+TGG 0.363606 5.2:+21554789 MS.gene027823:CDS
TCTACCTATCCACTAGTTCC+TGG 0.381163 5.2:+21555103 MS.gene027823:CDS
GCAGGGAGAAAGACGATATC+TGG 0.389267 5.2:+21556135 MS.gene027823:intron
GATATTTCACATCTGCTTTG+AGG 0.391986 5.2:-21556278 None:intergenic
CATTTAAAGTACAGTATTGC+GGG 0.393811 5.2:+21555035 MS.gene027823:CDS
GGGAGCTCCGGAGGCTTCTC+AGG 0.395546 5.2:-21555934 None:intergenic
ATAGGTATTGTTGGTCTTGG+TGG 0.404910 5.2:+21555571 MS.gene027823:CDS
ATTGTTGGTCTTGGTGGACT+TGG 0.407291 5.2:+21555577 MS.gene027823:CDS
CATCCTCTCTTACCTCTAGT+TGG 0.407705 5.2:+21555871 MS.gene027823:CDS
TTGTACAAAATTTAAGAACT+AGG 0.429877 5.2:-21556333 None:intergenic
ATATCACGGAAAACTTATAA+TGG 0.431863 5.2:+21555903 MS.gene027823:CDS
GATTATAGGCATGAGGTTGC+TGG 0.437448 5.2:+21555217 MS.gene027823:intron
ACGATATCTGGCAGTGGCAT+TGG 0.438228 5.2:+21556147 MS.gene027823:CDS
TCCAAAGAAGGCATTTGGTT+GGG 0.449991 5.2:+21554794 MS.gene027823:CDS
GTCGTGAACTTTCAGAGAAA+CGG 0.456414 5.2:+21554997 MS.gene027823:intron
AATATAGGTATTGTTGGTCT+TGG 0.457645 5.2:+21555568 MS.gene027823:CDS
GCTTCAAGTGGTAAGCCATC+AGG 0.468059 5.2:-21555469 None:intergenic
TCGTGAACTTTCAGAGAAAC+GGG 0.468716 5.2:+21554998 MS.gene027823:intron
GGTATGAATATAGGTATTGT+TGG 0.472768 5.2:+21555562 MS.gene027823:CDS
ATAGGCTGTGATGGGTACTT+TGG 0.476491 5.2:+21555819 MS.gene027823:intron
TGCTAAACACGATATAAAAC+CGG 0.477369 5.2:+21556206 MS.gene027823:CDS
AAAGTAGAGAAGTTCAAAGT+TGG 0.481176 5.2:+21555262 MS.gene027823:CDS
AGTGATGGAACCATCACATA+TGG 0.484543 5.2:+21555397 MS.gene027823:CDS
ATAGGTATGACTTCGATGTC+CGG 0.491179 5.2:-21556225 None:intergenic
GACAAGCCTGGTATGAATAT+AGG 0.491634 5.2:+21555553 MS.gene027823:CDS
TAAGAAATGAATCAGCTCCT+AGG 0.495743 5.2:-21555690 None:intergenic
ACCTTCTAGAGAAGTTGAAA+GGG 0.496182 5.2:-21554845 None:intergenic
CTTGGTGGACTTGGTCACTT+GGG 0.501069 5.2:+21555586 MS.gene027823:CDS
GCTGCTCCTCTCCTTTGTGC+TGG 0.501431 5.2:+21555493 MS.gene027823:CDS
TGGGCAGCAAGAGACACTTC+TGG 0.507758 5.2:+21554813 MS.gene027823:CDS
AAAGGAATGTGAGGGAGCTC+CGG 0.512634 5.2:-21555946 None:intergenic
AACCTTCTAGAGAAGTTGAA+AGG 0.514607 5.2:-21554846 None:intergenic
AGTTGGAGACAAAGTGGGTG+TGG 0.517700 5.2:+21555279 MS.gene027823:CDS
ATATCTGGCAGTGGCATTGG+AGG 0.523014 5.2:+21556150 MS.gene027823:CDS
TTTATAGTTCGCATTCCTGA+TGG 0.525602 5.2:+21555454 MS.gene027823:CDS
TGGAGGGTACTCTGATTCAA+TGG 0.526695 5.2:+21555417 MS.gene027823:CDS
CGGTGCACCTGAGAAGCCTC+CGG 0.529283 5.2:+21555927 MS.gene027823:CDS
ATCCAAAGAAGGCATTTGGT+TGG 0.531418 5.2:+21554793 MS.gene027823:CDS
TGAATTTGAACATCCAAAGA+AGG 0.532781 5.2:+21554782 MS.gene027823:CDS
CCCATTATTAAAGGAATGTG+AGG 0.542582 5.2:-21555955 None:intergenic
GTTGGATTATTAAAATATCA+CGG 0.550914 5.2:+21555889 MS.gene027823:CDS
GGACATCGAAGTCATACCTA+TGG 0.559852 5.2:+21556227 MS.gene027823:CDS
CACGGAAAACTTATAATGGT+CGG 0.562740 5.2:+21555907 MS.gene027823:CDS
CTACCTATCCACTAGTTCCT+GGG 0.565757 5.2:+21555104 MS.gene027823:CDS
CATACGTACCCAGGAACTAG+TGG 0.581134 5.2:-21555112 None:intergenic
GCATTTAAAGTACAGTATTG+CGG 0.588287 5.2:+21555034 MS.gene027823:CDS
GATATATGATTATAGGCATG+AGG 0.590572 5.2:+21555210 MS.gene027823:intron
GCTGGTATAGTCACAGAAGT+GGG 0.600918 5.2:+21555235 MS.gene027823:CDS
AAAGAAGCAATAGAAAACCT+AGG 0.602446 5.2:+21555673 MS.gene027823:CDS
TCATGACCAAGATAAAATGC+AGG 0.605547 5.2:+21555717 MS.gene027823:CDS
TGCTGGTATAGTCACAGAAG+TGG 0.607495 5.2:+21555234 MS.gene027823:CDS
AGAGTACCCTCCATATGTGA+TGG 0.608517 5.2:-21555407 None:intergenic
GATGGAACCATCACATATGG+AGG 0.610966 5.2:+21555400 MS.gene027823:CDS
GTTCAAAGTTGGAGACAAAG+TGG 0.616401 5.2:+21555273 MS.gene027823:CDS
GGAATGTGAGGGAGCTCCGG+AGG 0.622096 5.2:-21555943 None:intergenic
TATCATTGATACAGTTTCAG+CGG 0.623020 5.2:+21555846 MS.gene027823:CDS
GCTGTGTTAACATAATCCAT+AGG 0.623116 5.2:-21556243 None:intergenic
GTAATACCAGCACAAAGGAG+AGG 0.623289 5.2:-21555499 None:intergenic
AATCCAACTAGAGGTAAGAG+AGG 0.623627 5.2:-21555874 None:intergenic
GCTGTGATGGGTACTTTGGA+TGG 0.624358 5.2:+21555823 MS.gene027823:CDS
AGAAAGACGATATCTGGCAG+TGG 0.629634 5.2:+21556141 MS.gene027823:CDS
GTACCCAGGAACTAGTGGAT+AGG 0.637899 5.2:-21555107 None:intergenic
CCATTATTAAAGGAATGTGA+GGG 0.639713 5.2:-21555954 None:intergenic
TTCAAAGTTGGAGACAAAGT+GGG 0.640553 5.2:+21555274 MS.gene027823:CDS
GTTGGAGACAAAGTGGGTGT+GGG 0.640763 5.2:+21555280 MS.gene027823:CDS
TATAGTGCGAAATACAGTGA+TGG 0.641571 5.2:+21555382 MS.gene027823:CDS
AGAGGAGCAGCAGCTTCAAG+TGG 0.642094 5.2:-21555481 None:intergenic
TCGAGTCCAAAATATCGAAG+AGG 0.642096 5.2:-21555532 None:intergenic
CCTTCTAGAGAAGTTGAAAG+GGG 0.645104 5.2:-21554844 None:intergenic
TCGCGCATGCCAAAATTGTG+AGG 0.663146 5.2:+21555321 MS.gene027823:CDS
ACAATACCTATATTCATACC+AGG 0.667309 5.2:-21555559 None:intergenic
ATGGAACCATCACATATGGA+GGG 0.672614 5.2:+21555401 MS.gene027823:CDS
GTATTGCGGGATATGTCACT+CGG 0.700120 5.2:+21555048 MS.gene027823:CDS
ACACAGTAATACCAGCACAA+AGG 0.710961 5.2:-21555504 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTTATAATAATAATCTTAAT+TGG + chr5.2:21555758-21555777 MS.gene027823:intron 10.0%
!! TAAGATTATTATTATAACTT+TGG - chr5.2:21555756-21555775 None:intergenic 10.0%
!!! ATTTTGATGATATTATTTAT+TGG + chr5.2:21556113-21556132 MS.gene027823:intron 10.0%
!! AATTAAAAATTCCTAAGATA+TGG - chr5.2:21555158-21555177 None:intergenic 15.0%
!! TCTAATTGATATATGATTAT+AGG + chr5.2:21555203-21555222 MS.gene027823:intron 15.0%
!!! ATTAATGTCAATGATTTTAT+AGG + chr5.2:21555801-21555820 MS.gene027823:intron 15.0%
!!! TTATTTTCATAGGTATAAAA+GGG - chr5.2:21556083-21556102 None:intergenic 15.0%
!!! TTGACAAAATAATTATTTCT+TGG + chr5.2:21554971-21554990 MS.gene027823:intron 15.0%
!!! TTTATTTTCATAGGTATAAA+AGG - chr5.2:21556084-21556103 None:intergenic 15.0%
!! AAAAGATGTATAAAATGAAG+AGG - chr5.2:21554886-21554905 None:intergenic 20.0%
!! GTTGGATTATTAAAATATCA+CGG + chr5.2:21555889-21555908 MS.gene027823:CDS 20.0%
!! TATATGTTTGTGTTTGTTTA+GGG - chr5.2:21555366-21555385 None:intergenic 20.0%
!! TCATATGATCAAAAATGAAT+GGG + chr5.2:21555075-21555094 MS.gene027823:CDS 20.0%
!! TTCATATGATCAAAAATGAA+TGG + chr5.2:21555074-21555093 MS.gene027823:CDS 20.0%
!!! GTTCATACATTTATTTTCAT+AGG - chr5.2:21556093-21556112 None:intergenic 20.0%
!!! TATTTTAATAATCCAACTAG+AGG - chr5.2:21555886-21555905 None:intergenic 20.0%
! AACAACTTACCCATTATTAA+AGG - chr5.2:21555967-21555986 None:intergenic 25.0%
! ACTTTAACAAACCATATCTT+AGG + chr5.2:21555144-21555163 MS.gene027823:intron 25.0%
! ATATCACGGAAAACTTATAA+TGG + chr5.2:21555903-21555922 MS.gene027823:CDS 25.0%
! CAATTAGAATAAAATTGCAG+TGG - chr5.2:21555191-21555210 None:intergenic 25.0%
! CATATGATCAAAAATGAATG+GGG + chr5.2:21555076-21555095 MS.gene027823:CDS 25.0%
! CTATATGTTTGTGTTTGTTT+AGG - chr5.2:21555367-21555386 None:intergenic 25.0%
! GATGATATTATTTATTGGCA+GGG + chr5.2:21556118-21556137 MS.gene027823:intron 25.0%
! TGATGATATTATTTATTGGC+AGG + chr5.2:21556117-21556136 MS.gene027823:intron 25.0%
!! TTTATACCTGCATTTTATCT+TGG - chr5.2:21555726-21555745 None:intergenic 25.0%
!!! TATCGATTTGTGATTGATAT+TGG + chr5.2:21556297-21556316 MS.gene027823:CDS 25.0%
AAAGAAGCAATAGAAAACCT+AGG + chr5.2:21555673-21555692 MS.gene027823:CDS 30.0%
AAAGTAGAGAAGTTCAAAGT+TGG + chr5.2:21555262-21555281 MS.gene027823:CDS 30.0%
ACAATACCTATATTCATACC+AGG - chr5.2:21555562-21555581 None:intergenic 30.0%
AGATGTATAAAATGAAGAGG+TGG - chr5.2:21554883-21554902 None:intergenic 30.0%
ATTGCTTCTTTCTCTTTGTT+GGG - chr5.2:21555664-21555683 None:intergenic 30.0%
CCATTATTAAAGGAATGTGA+GGG - chr5.2:21555957-21555976 None:intergenic 30.0%
CCTCACATTCCTTTAATAAT+GGG + chr5.2:21555955-21555974 MS.gene027823:CDS 30.0%
GATATATGATTATAGGCATG+AGG + chr5.2:21555210-21555229 MS.gene027823:intron 30.0%
TATCATTGATACAGTTTCAG+CGG + chr5.2:21555846-21555865 MS.gene027823:CDS 30.0%
TATTGCTTCTTTCTCTTTGT+TGG - chr5.2:21555665-21555684 None:intergenic 30.0%
TGAATTTGAACATCCAAAGA+AGG + chr5.2:21554782-21554801 MS.gene027823:CDS 30.0%
TGCTAAACACGATATAAAAC+CGG + chr5.2:21556206-21556225 MS.gene027823:CDS 30.0%
TTCTTTGGATGTTCAAATTC+AGG - chr5.2:21554783-21554802 None:intergenic 30.0%
! AATATAGGTATTGTTGGTCT+TGG + chr5.2:21555568-21555587 MS.gene027823:CDS 30.0%
! AATGATTTTATAGGCTGTGA+TGG + chr5.2:21555810-21555829 MS.gene027823:intron 30.0%
! ATGATTTTATAGGCTGTGAT+GGG + chr5.2:21555811-21555830 MS.gene027823:intron 30.0%
! CAAGATTTTCCTCACAATTT+TGG - chr5.2:21555333-21555352 None:intergenic 30.0%
! CATTTAAAGTACAGTATTGC+GGG + chr5.2:21555035-21555054 MS.gene027823:CDS 30.0%
! GCATTTAAAGTACAGTATTG+CGG + chr5.2:21555034-21555053 MS.gene027823:CDS 30.0%
! GGTATGAATATAGGTATTGT+TGG + chr5.2:21555562-21555581 MS.gene027823:CDS 30.0%
! TATAGTCCTCTTCGATATTT+TGG + chr5.2:21555526-21555545 MS.gene027823:CDS 30.0%
CACGGAAAACTTATAATGGT+CGG + chr5.2:21555907-21555926 MS.gene027823:CDS 35.0%
CCCATTATTAAAGGAATGTG+AGG - chr5.2:21555958-21555977 None:intergenic 35.0%
CCCTCACATTCCTTTAATAA+TGG + chr5.2:21555954-21555973 MS.gene027823:CDS 35.0%
GATATTTCACATCTGCTTTG+AGG - chr5.2:21556281-21556300 None:intergenic 35.0%
GATTGTGATCGATATGTTCA+TGG + chr5.2:21556051-21556070 MS.gene027823:intron 35.0%
GCTGTGTTAACATAATCCAT+AGG - chr5.2:21556246-21556265 None:intergenic 35.0%
GTATAAAATGAAGAGGTGGA+TGG - chr5.2:21554879-21554898 None:intergenic 35.0%
TAAGAAATGAATCAGCTCCT+AGG - chr5.2:21555693-21555712 None:intergenic 35.0%
TATAGTGCGAAATACAGTGA+TGG + chr5.2:21555382-21555401 MS.gene027823:CDS 35.0%
TCATGACCAAGATAAAATGC+AGG + chr5.2:21555717-21555736 MS.gene027823:CDS 35.0%
TGTTGTGAAAAATGTTCCCA+CGG + chr5.2:21555983-21556002 MS.gene027823:intron 35.0%
TTCAAAGTTGGAGACAAAGT+GGG + chr5.2:21555274-21555293 MS.gene027823:CDS 35.0%
TTTATAGTTCGCATTCCTGA+TGG + chr5.2:21555454-21555473 MS.gene027823:CDS 35.0%
! AACCTTCTAGAGAAGTTGAA+AGG - chr5.2:21554849-21554868 None:intergenic 35.0%
! ACCTTCTAGAGAAGTTGAAA+GGG - chr5.2:21554848-21554867 None:intergenic 35.0%
! GTGAAGTTTGCAAAAGCTTT+TGG + chr5.2:21555610-21555629 MS.gene027823:CDS 35.0%
AAATTCAAACACTAGAGCCG+TGG - chr5.2:21556003-21556022 None:intergenic 40.0%
AATCCAACTAGAGGTAAGAG+AGG - chr5.2:21555877-21555896 None:intergenic 40.0%
AATTCAAACACTAGAGCCGT+GGG - chr5.2:21556002-21556021 None:intergenic 40.0%
ACACAGTAATACCAGCACAA+AGG - chr5.2:21555507-21555526 None:intergenic 40.0%
AGAAACGGGTGAAAAAGATG+TGG + chr5.2:21555012-21555031 MS.gene027823:CDS 40.0%
ATAGGTATGACTTCGATGTC+CGG - chr5.2:21556228-21556247 None:intergenic 40.0%
ATGGAACCATCACATATGGA+GGG + chr5.2:21555401-21555420 MS.gene027823:CDS 40.0%
GACAAGCCTGGTATGAATAT+AGG + chr5.2:21555553-21555572 MS.gene027823:CDS 40.0%
GTCGTGAACTTTCAGAGAAA+CGG + chr5.2:21554997-21555016 MS.gene027823:intron 40.0%
GTTCAAAGTTGGAGACAAAG+TGG + chr5.2:21555273-21555292 MS.gene027823:CDS 40.0%
TAAAATGAAGAGGTGGATGG+AGG - chr5.2:21554876-21554895 None:intergenic 40.0%
TCGAGTCCAAAATATCGAAG+AGG - chr5.2:21555535-21555554 None:intergenic 40.0%
TCGTGAACTTTCAGAGAAAC+GGG + chr5.2:21554998-21555017 MS.gene027823:intron 40.0%
! AGTGATGGAACCATCACATA+TGG + chr5.2:21555397-21555416 MS.gene027823:CDS 40.0%
! CCTTCTAGAGAAGTTGAAAG+GGG - chr5.2:21554847-21554866 None:intergenic 40.0%
!! ATAGGTATTGTTGGTCTTGG+TGG + chr5.2:21555571-21555590 MS.gene027823:CDS 40.0%
!! ATCCAAAGAAGGCATTTGGT+TGG + chr5.2:21554793-21554812 MS.gene027823:CDS 40.0%
!! GAACATCCAAAGAAGGCATT+TGG + chr5.2:21554789-21554808 MS.gene027823:CDS 40.0%
!! TCCAAAGAAGGCATTTGGTT+GGG + chr5.2:21554794-21554813 MS.gene027823:CDS 40.0%
AGAAAGACGATATCTGGCAG+TGG + chr5.2:21556141-21556160 MS.gene027823:CDS 45.0%
CATCCTCTCTTACCTCTAGT+TGG + chr5.2:21555871-21555890 MS.gene027823:CDS 45.0%
CCCCTTTCAACTTCTCTAGA+AGG + chr5.2:21554844-21554863 MS.gene027823:CDS 45.0%
CTACCTATCCACTAGTTCCT+GGG + chr5.2:21555104-21555123 MS.gene027823:CDS 45.0%
GATGGAACCATCACATATGG+AGG + chr5.2:21555400-21555419 MS.gene027823:CDS 45.0%
GATTATAGGCATGAGGTTGC+TGG + chr5.2:21555217-21555236 MS.gene027823:intron 45.0%
GCTGGTATAGTCACAGAAGT+GGG + chr5.2:21555235-21555254 MS.gene027823:CDS 45.0%
GGACATCGAAGTCATACCTA+TGG + chr5.2:21556227-21556246 MS.gene027823:CDS 45.0%
GTAATACCAGCACAAAGGAG+AGG - chr5.2:21555502-21555521 None:intergenic 45.0%
GTATTGCGGGATATGTCACT+CGG + chr5.2:21555048-21555067 MS.gene027823:CDS 45.0%
TCTACCTATCCACTAGTTCC+TGG + chr5.2:21555103-21555122 MS.gene027823:CDS 45.0%
TGCTGGTATAGTCACAGAAG+TGG + chr5.2:21555234-21555253 MS.gene027823:CDS 45.0%
! AGAGTACCCTCCATATGTGA+TGG - chr5.2:21555410-21555429 None:intergenic 45.0%
!! ATAGGCTGTGATGGGTACTT+TGG + chr5.2:21555819-21555838 MS.gene027823:intron 45.0%
!! ATTGTTGGTCTTGGTGGACT+TGG + chr5.2:21555577-21555596 MS.gene027823:CDS 45.0%
!! TATTTTGGACTCGACAAGCC+TGG + chr5.2:21555541-21555560 MS.gene027823:CDS 45.0%
!! TGGAGGGTACTCTGATTCAA+TGG + chr5.2:21555417-21555436 MS.gene027823:CDS 45.0%
AAAGGAATGTGAGGGAGCTC+CGG - chr5.2:21555949-21555968 None:intergenic 50.0%
ACGATATCTGGCAGTGGCAT+TGG + chr5.2:21556147-21556166 MS.gene027823:CDS 50.0%
AGTTCGAAGCATACGTACCC+AGG - chr5.2:21555124-21555143 None:intergenic 50.0%
AGTTGGAGACAAAGTGGGTG+TGG + chr5.2:21555279-21555298 MS.gene027823:CDS 50.0%
CATACGTACCCAGGAACTAG+TGG - chr5.2:21555115-21555134 None:intergenic 50.0%
GCAGGGAGAAAGACGATATC+TGG + chr5.2:21556135-21556154 MS.gene027823:intron 50.0%
GCCCAACCAAATGCCTTCTT+TGG - chr5.2:21554798-21554817 None:intergenic 50.0%
GCTTCAAGTGGTAAGCCATC+AGG - chr5.2:21555472-21555491 None:intergenic 50.0%
GTTGGAGACAAAGTGGGTGT+GGG + chr5.2:21555280-21555299 MS.gene027823:CDS 50.0%
TCGCGCATGCCAAAATTGTG+AGG + chr5.2:21555321-21555340 MS.gene027823:CDS 50.0%
! ATATCTGGCAGTGGCATTGG+AGG + chr5.2:21556150-21556169 MS.gene027823:CDS 50.0%
! GTACCCAGGAACTAGTGGAT+AGG - chr5.2:21555110-21555129 None:intergenic 50.0%
!! CTTGGTGGACTTGGTCACTT+GGG + chr5.2:21555586-21555605 MS.gene027823:CDS 50.0%
!! GCTGTGATGGGTACTTTGGA+TGG + chr5.2:21555823-21555842 MS.gene027823:CDS 50.0%
!! TCTTGGTGGACTTGGTCACT+TGG + chr5.2:21555585-21555604 MS.gene027823:CDS 50.0%
AGAGGAGCAGCAGCTTCAAG+TGG - chr5.2:21555484-21555503 None:intergenic 55.0%
TGGGCAGCAAGAGACACTTC+TGG + chr5.2:21554813-21554832 MS.gene027823:CDS 55.0%
GCTGCTCCTCTCCTTTGTGC+TGG + chr5.2:21555493-21555512 MS.gene027823:CDS 60.0%
CGGTGCACCTGAGAAGCCTC+CGG + chr5.2:21555927-21555946 MS.gene027823:CDS 65.0%
GGAATGTGAGGGAGCTCCGG+AGG - chr5.2:21555946-21555965 None:intergenic 65.0%
GGGAGCTCCGGAGGCTTCTC+AGG - chr5.2:21555937-21555956 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 21554768 21556344 21554768 ID=MS.gene027823
chr5.2 mRNA 21554768 21556344 21554768 ID=MS.gene027823.t1;Parent=MS.gene027823
chr5.2 exon 21554768 21554865 21554768 ID=MS.gene027823.t1.exon1;Parent=MS.gene027823.t1
chr5.2 CDS 21554768 21554865 21554768 ID=cds.MS.gene027823.t1;Parent=MS.gene027823.t1
chr5.2 exon 21555012 21555125 21555012 ID=MS.gene027823.t1.exon2;Parent=MS.gene027823.t1
chr5.2 CDS 21555012 21555125 21555012 ID=cds.MS.gene027823.t1;Parent=MS.gene027823.t1
chr5.2 exon 21555225 21555738 21555225 ID=MS.gene027823.t1.exon3;Parent=MS.gene027823.t1
chr5.2 CDS 21555225 21555738 21555225 ID=cds.MS.gene027823.t1;Parent=MS.gene027823.t1
chr5.2 exon 21555823 21555976 21555823 ID=MS.gene027823.t1.exon4;Parent=MS.gene027823.t1
chr5.2 CDS 21555823 21555976 21555823 ID=cds.MS.gene027823.t1;Parent=MS.gene027823.t1
chr5.2 exon 21556139 21556344 21556139 ID=MS.gene027823.t1.exon5;Parent=MS.gene027823.t1
chr5.2 CDS 21556139 21556344 21556139 ID=cds.MS.gene027823.t1;Parent=MS.gene027823.t1
Gene Sequence

>MS.gene027823

ATGGCAACACAACCTGAATTTGAACATCCAAAGAAGGCATTTGGTTGGGCAGCAAGAGACACTTCTGGTGTTCTCTCCCCTTTCAACTTCTCTAGAAGGTTCATTCCTCCATCCACCTCTTCATTTTATACATCTTTTAAACTAACAAAGTGCTAACAATTGTTGTGAAGACAATAATATTTTAATATGTTGAGAACAATATGTTGACAAAATAATTATTTCTTGGTGTGTCGTGAACTTTCAGAGAAACGGGTGAAAAAGATGTGGCATTTAAAGTACAGTATTGCGGGATATGTCACTCGGATCTTCATATGATCAAAAATGAATGGGGCATGTCTACCTATCCACTAGTTCCTGGGTACGTATGCTTCGAACTACTTTAACAAACCATATCTTAGGAATTTTTAATTTTACTATTTTCCACTGCAATTTTATTCTAATTGATATATGATTATAGGCATGAGGTTGCTGGTATAGTCACAGAAGTGGGAAGCAAAGTAGAGAAGTTCAAAGTTGGAGACAAAGTGGGTGTGGGATGCTTAGTTGATTCATGTCGCGCATGCCAAAATTGTGAGGAAAATCTTGAGAATTATTGCCCTAAACAAACACAAACATATAGTGCGAAATACAGTGATGGAACCATCACATATGGAGGGTACTCTGATTCAATGGTTGCAGATGAGCACTTTATAGTTCGCATTCCTGATGGCTTACCACTTGAAGCTGCTGCTCCTCTCCTTTGTGCTGGTATTACTGTGTATAGTCCTCTTCGATATTTTGGACTCGACAAGCCTGGTATGAATATAGGTATTGTTGGTCTTGGTGGACTTGGTCACTTGGGTGTGAAGTTTGCAAAAGCTTTTGGTGCTAATGTAACAGTAATTAGTACATCACCCAACAAAGAGAAAGAAGCAATAGAAAACCTAGGAGCTGATTCATTTCTTATAAGTCATGACCAAGATAAAATGCAGGTATAAATACAAAACCAAAGTTATAATAATAATCTTAATTGGAAATTGTAATTGATTAACTTATTAATGTCAATGATTTTATAGGCTGTGATGGGTACTTTGGATGGTATCATTGATACAGTTTCAGCGGATCATCCTCTCTTACCTCTAGTTGGATTATTAAAATATCACGGAAAACTTATAATGGTCGGTGCACCTGAGAAGCCTCCGGAGCTCCCTCACATTCCTTTAATAATGGGTAAGTTGTTGTGAAAAATGTTCCCACGGCTCTAGTGTTTGAATTTTATCATAACTATCTCTAAATTTATGATTGATTGTGATCGATATGTTCATGGTTTAAACCCTTTTATACCTATGAAAATAAATGTATGAACATTTTGATGATATTATTTATTGGCAGGGAGAAAGACGATATCTGGCAGTGGCATTGGAGGCATGAAAGAGACACAAGAAATGATTGATTTTGCTGCTAAACACGATATAAAACCGGACATCGAAGTCATACCTATGGATTATGTTAACACAGCAATGAAGCGACTCCTCAAAGCAGATGTGAAATATCGATTTGTGATTGATATTGGAAACACTTTAAAACCTAGTTCTTAA
Protein sequence

>MS.gene027823.t1

MATQPEFEHPKKAFGWAARDTSGVLSPFNFSRRETGEKDVAFKVQYCGICHSDLHMIKNEWGMSTYPLVPGHEVAGIVTEVGSKVEKFKVGDKVGVGCLVDSCRACQNCEENLENYCPKQTQTYSAKYSDGTITYGGYSDSMVADEHFIVRIPDGLPLEAAAPLLCAGITVYSPLRYFGLDKPGMNIGIVGLGGLGHLGVKFAKAFGANVTVISTSPNKEKEAIENLGADSFLISHDQDKMQAVMGTLDGIIDTVSADHPLLPLVGLLKYHGKLIMVGAPEKPPELPHIPLIMGRKTISGSGIGGMKETQEMIDFAAKHDIKPDIEVIPMDYVNTAMKRLLKADVKYRFVIDIGNTLKPSS