AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene069153

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069153.t1 MTR_5g031190 98.061 361 7 0 1 361 1 361 0.0 729
MS.gene069153.t1 MTR_5g031180 86.981 361 47 0 1 361 1 361 0.0 610
MS.gene069153.t1 MTR_8g068190 81.389 360 67 0 1 360 1 360 0.0 579
MS.gene069153.t1 MTR_5g031250 77.562 361 81 0 1 361 1 361 0.0 573
MS.gene069153.t1 MTR_5g031390 76.731 361 84 0 1 361 1 361 0.0 572
MS.gene069153.t1 MTR_5g031230 76.177 361 86 0 1 361 1 361 0.0 566
MS.gene069153.t1 MTR_5g031310 77.337 353 80 0 8 360 9 361 0.0 563
MS.gene069153.t1 MTR_5g031360 76.923 351 81 0 10 360 12 362 0.0 560
MS.gene069153.t1 MTR_4g115300 75.069 361 90 0 1 361 1 361 0.0 558
MS.gene069153.t1 MTR_5g031305 76.771 353 82 0 8 360 9 361 0.0 557
MS.gene069153.t1 MTR_5g031300 73.729 354 93 0 8 361 9 362 0.0 545
MS.gene069153.t1 MTR_5g031210 74.515 361 92 0 1 361 1 361 0.0 537
MS.gene069153.t1 MTR_8g092630 64.146 357 127 1 3 359 60 415 4.38e-153 437
MS.gene069153.t1 MTR_5g031430 59.777 358 144 0 1 358 1 358 2.06e-147 421
MS.gene069153.t1 MTR_1g025950 57.627 354 149 1 8 360 7 360 3.91e-137 395
MS.gene069153.t1 MTR_5g031250 76.349 241 57 0 1 241 1 241 1.15e-132 379
MS.gene069153.t1 MTR_1g107425 52.770 343 162 0 11 353 9 351 1.76e-124 362
MS.gene069153.t1 MTR_2g009810 54.046 346 157 2 14 358 62 406 7.98e-123 360
MS.gene069153.t1 MTR_1g107425 52.083 288 138 0 11 298 9 296 6.09e-99 295
MS.gene069153.t1 MTR_2g009820 50.400 250 114 4 116 358 8 254 6.25e-69 217
MS.gene069153.t1 MTR_5g031290 73.684 114 30 0 247 360 80 193 1.45e-54 178
MS.gene069153.t1 MTR_5g031290 80.000 80 15 1 1 79 1 80 1.42e-40 142
MS.gene069153.t1 MTR_8g095220 26.786 280 175 7 25 278 68 343 1.29e-23 101
MS.gene069153.t1 MTR_8g095220 28.099 242 149 6 25 245 68 305 3.57e-23 98.6
MS.gene069153.t1 MTR_3g089977 24.074 378 243 15 11 354 12 379 3.10e-18 85.5
MS.gene069153.t1 MTR_3g089940 24.457 368 235 14 11 344 12 370 5.22e-18 84.7
MS.gene069153.t1 MTR_0001s0660 26.159 302 199 9 38 318 35 333 6.76e-18 85.1
MS.gene069153.t1 MTR_3g089940 24.412 340 217 12 39 344 37 370 8.86e-18 84.0
MS.gene069153.t1 MTR_3g089970 23.824 340 219 12 39 344 38 371 3.32e-16 79.3
MS.gene069153.t1 MTR_3g089940 24.702 336 218 12 11 319 12 339 4.21e-16 79.0
MS.gene069153.t1 MTR_6g466320 26.000 300 198 9 1 288 6 293 6.37e-16 78.6
MS.gene069153.t1 MTR_6g465010 26.000 300 198 9 1 288 6 293 6.37e-16 78.6
MS.gene069153.t1 MTR_3g089940 24.405 336 219 12 11 319 12 339 7.40e-16 78.2
MS.gene069153.t1 MTR_6g465010 26.224 286 188 8 15 288 3 277 9.56e-16 77.8
MS.gene069153.t1 MTR_7g068320 26.361 349 220 13 39 353 48 393 8.64e-14 72.4
MS.gene069153.t1 MTR_5g045710 24.918 305 200 9 38 318 35 334 1.30e-13 71.6
MS.gene069153.t1 MTR_1g069430 23.810 336 227 8 1 312 1 331 1.50e-13 71.6
MS.gene069153.t1 MTR_7g099040 23.899 318 207 9 38 327 37 347 1.50e-12 68.6
MS.gene069153.t1 MTR_3g099460 26.537 309 197 8 39 319 37 343 3.49e-11 64.3
MS.gene069153.t1 MTR_3g099460 26.537 309 197 8 39 319 44 350 3.78e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069153.t1 AT4G37980 72.000 350 98 0 11 360 7 356 2.35e-173 486
MS.gene069153.t1 AT4G37970 65.915 355 121 0 6 360 7 361 1.93e-172 484
MS.gene069153.t1 AT4G37990 72.571 350 96 0 11 360 7 356 4.39e-172 483
MS.gene069153.t1 AT4G39330 63.585 357 130 0 1 357 1 357 1.89e-169 476
MS.gene069153.t1 AT2G21730 61.891 349 131 2 11 357 5 353 8.41e-157 445
MS.gene069153.t1 AT2G21890 60.920 348 135 1 11 357 5 352 2.38e-155 441
MS.gene069153.t1 AT4G39330 62.783 309 115 0 1 309 1 309 1.59e-139 399
MS.gene069153.t1 AT4G37980 72.887 284 77 0 11 294 7 290 1.24e-135 389
MS.gene069153.t1 AT4G37970 65.052 289 101 0 6 294 7 295 4.57e-133 382
MS.gene069153.t1 AT3G19450 54.335 346 158 0 11 356 9 354 8.20e-119 348
MS.gene069153.t1 AT1G72680 48.324 358 182 2 1 358 1 355 1.15e-113 335
MS.gene069153.t1 AT4G34230 52.975 353 165 1 8 359 5 357 1.02e-108 322
MS.gene069153.t1 AT4G34230 52.691 353 166 1 8 359 5 357 8.93e-108 320
MS.gene069153.t1 AT4G37970 64.557 237 84 0 6 242 7 243 1.76e-105 310
MS.gene069153.t1 AT4G37970 64.557 237 84 0 6 242 7 243 1.95e-105 310
MS.gene069153.t1 AT5G63620 27.491 291 181 7 15 278 58 345 3.46e-25 105
MS.gene069153.t1 AT5G63620 27.491 291 181 7 15 278 58 345 3.49e-25 105
MS.gene069153.t1 AT5G63620 27.888 251 158 5 15 244 58 306 4.87e-24 101
MS.gene069153.t1 AT5G51970 26.812 276 179 8 15 278 21 285 1.08e-16 80.9
MS.gene069153.t1 AT5G51970 26.812 276 179 8 15 278 21 285 1.08e-16 80.9
MS.gene069153.t1 AT1G64710 24.516 310 199 10 39 318 13 317 4.85e-16 79.0
MS.gene069153.t1 AT1G64710 24.516 310 199 10 39 318 36 340 5.83e-16 79.0
MS.gene069153.t1 AT5G43940 24.262 305 205 6 39 319 36 338 7.96e-16 78.6
MS.gene069153.t1 AT5G43940 24.262 305 205 6 39 319 48 350 8.69e-16 78.6
MS.gene069153.t1 AT1G77120 23.631 347 230 13 39 354 36 378 3.50e-15 76.6
MS.gene069153.t1 AT1G32780 25.000 364 222 14 39 358 37 393 5.67e-13 70.1

Find 96 sgRNAs with CRISPR-Local

Find 154 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATAGCCCAATGAAACATTT+TGG 0.194725 5.3:-20247135 MS.gene069153:CDS
GTGGTTGGTCTTGGTGGATT+AGG 0.267262 5.3:-20247084 MS.gene069153:CDS
CTTGGTCTATTGAAGAATAA+TGG 0.272969 5.3:-20246732 MS.gene069153:CDS
GTGAAGTTCGCCAAAGCTTT+TGG 0.308390 5.3:-20247051 MS.gene069153:CDS
TCTAATCCAAAATGTTTCAT+TGG 0.321961 5.3:+20247129 None:intergenic
GCTGCTCCTCTCCTTTGTGC+TGG 0.345949 5.3:-20247168 MS.gene069153:CDS
CTCCTTTCAAGTTCTCTAGA+AGG 0.347990 5.3:-20248273 MS.gene069153:intron
GTTGGTGTGGGGTGCATGGT+TGG 0.355897 5.3:-20247369 MS.gene069153:CDS
GCTGTAATGGGCACGTTTGA+TGG 0.372955 5.3:-20246798 MS.gene069153:CDS
GGAAACTCCAAAGGCTTCTC+TGG 0.377400 5.3:+20246687 None:intergenic
CTAATCCAAAATGTTTCATT+GGG 0.384957 5.3:+20247130 None:intergenic
CCCACAATCAAAGAAAATAC+TGG 0.392755 5.3:+20246666 None:intergenic
GAATAATGGTAAACTTGTTA+TGG 0.398732 5.3:-20246718 MS.gene069153:CDS
TACCTTTACACCAAAAGCTT+TGG 0.401200 5.3:+20247041 None:intergenic
GATAGTGCCATCAACATTCT+TGG 0.402024 5.3:+20247272 None:intergenic
CAGGGTTGGTGTGGGGTGCA+TGG 0.404978 5.3:-20247373 MS.gene069153:CDS
CAATATGTTATAGGCTGTAA+TGG 0.405959 5.3:-20246811 MS.gene069153:intron
ACTGGAACAACCTCAATATC+AGG 0.408861 5.3:+20246472 None:intergenic
AGACCAAGCAAAGGCATTAG+AGG 0.414126 5.3:+20246747 None:intergenic
ACTACTTACCCAGTAGTTCC+TGG 0.414223 5.3:-20247574 MS.gene069153:CDS
ACAATTGGTGGGAGTATGAT+TGG 0.420703 5.3:-20246550 MS.gene069153:CDS
TATTGGTACGCACCTTCATC+TGG 0.421150 5.3:+20246932 None:intergenic
TCTTGGTGGATTAGGTCATA+TGG 0.422443 5.3:-20247076 MS.gene069153:CDS
CATATAGGTGTGGTTGGTCT+TGG 0.424100 5.3:-20247093 MS.gene069153:CDS
CTGCTCCTCTCCTTTGTGCT+GGG 0.424848 5.3:-20247167 MS.gene069153:CDS
TACAGTTCCAAGAATGTTGA+TGG 0.430392 5.3:-20247279 MS.gene069153:CDS
ATAGGTGTGGTTGGTCTTGG+TGG 0.433205 5.3:-20247090 MS.gene069153:CDS
TATCGATTTGTTATTGACAT+TGG 0.438397 5.3:-20246400 MS.gene069153:CDS
CATCCTCTAATGCCTTTGCT+TGG 0.446345 5.3:-20246750 MS.gene069153:CDS
ATGCTCCGTTTCTGGATTTG+AGG 0.446888 5.3:+20248345 None:intergenic
CTTAGGAGCTGACTCATTTG+TGG 0.449060 5.3:-20246971 MS.gene069153:CDS
TGGAGCTCCAGAGAAGCCTT+TGG 0.454095 5.3:-20246694 MS.gene069153:CDS
TTAGAGTACCCAGGAACTAC+TGG 0.455836 5.3:+20247565 None:intergenic
AATATGTTATAGGCTGTAAT+GGG 0.459443 5.3:-20246810 MS.gene069153:intron
AAAACTTCAAAGTTGGAGAC+AGG 0.459613 5.3:-20247392 MS.gene069153:CDS
TGTTGAAGACATGAAGTTGC+TGG 0.463326 5.3:-20247444 MS.gene069153:intron
AGCAGCAGCATCAAGTGATA+AGG 0.468455 5.3:+20247185 None:intergenic
AATGGTAAACTTGTTATGGT+TGG 0.469139 5.3:-20246714 MS.gene069153:CDS
CACCACAACTTTGACATGAA+TGG 0.477319 5.3:+20247340 None:intergenic
AGTTGGAGACAGGGTTGGTG+TGG 0.488731 5.3:-20247382 MS.gene069153:CDS
AAGCTTTCGGATGGGCAGCT+AGG 0.490753 5.3:-20248315 MS.gene069153:CDS
GCATCAAGTGATAAGGCATC+AGG 0.495160 5.3:+20247192 None:intergenic
GTGATAAGGCATCAGGAATA+CGG 0.500674 5.3:+20247199 None:intergenic
AGCTTTCGGATGGGCAGCTA+GGG 0.501441 5.3:-20248314 MS.gene069153:CDS
GACAAACCTGGACTGCATAT+AGG 0.502372 5.3:-20247108 MS.gene069153:CDS
TGGGCAGCTAGGGACACATC+TGG 0.503109 5.3:-20248304 MS.gene069153:CDS
GAGTATGATTGGAGGGATGA+AGG 0.509991 5.3:-20246539 MS.gene069153:CDS
TTTCAGGGAAACAGGGGAGA+AGG 0.511637 5.3:-20247671 MS.gene069153:intron
GTTGATGGCACTATCACCTA+TGG 0.515552 5.3:-20247264 MS.gene069153:CDS
GGAAACAGGGGAGAAGGATG+TGG 0.515934 5.3:-20247665 MS.gene069153:CDS
GTTGGAGACAGGGTTGGTGT+GGG 0.523836 5.3:-20247381 MS.gene069153:CDS
TAGAGAACTTGAAAGGAGAA+AGG 0.526536 5.3:+20248278 None:intergenic
GTCAGCTCCTAAGTGTTCAA+TGG 0.541539 5.3:+20246981 None:intergenic
TTGGCAGGGAGAAAGACAAT+TGG 0.543249 5.3:-20246565 MS.gene069153:intron
GCAGGGAGAAAGACAATTGG+TGG 0.544871 5.3:-20246562 MS.gene069153:intron
TGACATTGGAAACACAATGA+AGG 0.546508 5.3:-20246386 MS.gene069153:CDS
TAGAGGATGGAGTGCTGAAA+CGG 0.546944 5.3:+20246764 None:intergenic
TGATATTGAGGTTGTTCCAG+TGG 0.550536 5.3:-20246470 MS.gene069153:CDS
GCAGTATTTACATAATCCAC+TGG 0.550557 5.3:+20246454 None:intergenic
ACCACACCTATATGCAGTCC+AGG 0.552769 5.3:+20247102 None:intergenic
AAGGAAGCCATTGAACACTT+AGG 0.565621 5.3:-20246988 MS.gene069153:CDS
TGGCGTAAAACCTGATATTG+AGG 0.570134 5.3:-20246482 MS.gene069153:CDS
AATGGCCTCAAATCCAGAAA+CGG 0.577912 5.3:-20248350 MS.gene069153:CDS
CAAGCAAAGGCATTAGAGGA+TGG 0.578766 5.3:+20246751 None:intergenic
TTCAAAGTTGGAGACAGGGT+TGG 0.579645 5.3:-20247387 MS.gene069153:CDS
GAAAATACTGGAAACTCCAA+AGG 0.580584 5.3:+20246678 None:intergenic
CTACTTACCCAGTAGTTCCT+GGG 0.589206 5.3:-20247573 MS.gene069153:intron
TTGGTGGGAGTATGATTGGA+GGG 0.589557 5.3:-20246546 MS.gene069153:CDS
ATTGGTGGGAGTATGATTGG+AGG 0.590145 5.3:-20246547 MS.gene069153:CDS
TCACATGGCTAAGAATGAGT+GGG 0.592665 5.3:-20247602 MS.gene069153:CDS
AGTGACAGAGGTGGGAAGCA+AGG 0.592868 5.3:-20247418 MS.gene069153:CDS
GGACTGCATATAGGTGTGGT+TGG 0.595201 5.3:-20247099 MS.gene069153:CDS
TACCTTCTAGAGAACTTGAA+AGG 0.595565 5.3:+20248271 None:intergenic
TTCACATGGCTAAGAATGAG+TGG 0.604893 5.3:-20247603 MS.gene069153:CDS
TGCCATTCATGTCAAAGTTG+TGG 0.605059 5.3:-20247342 MS.gene069153:CDS
GGTGTCAGAGTAGCCTCCAT+AGG 0.609083 5.3:+20247248 None:intergenic
GGATTATGTAAATACTGCAA+TGG 0.611722 5.3:-20246449 MS.gene069153:CDS
AAACTTCAAAGTTGGAGACA+GGG 0.615703 5.3:-20247391 MS.gene069153:CDS
TTGGAGACAGGGTTGGTGTG+GGG 0.621846 5.3:-20247380 MS.gene069153:CDS
TAGAGTACCCAGGAACTACT+GGG 0.622352 5.3:+20247566 None:intergenic
TTCTTCAATAGACCAAGCAA+AGG 0.622643 5.3:+20246738 None:intergenic
ACTCATTCTTAGCCATGTGA+AGG 0.631347 5.3:+20247605 None:intergenic
TCGCGAACAAGACCAGATGA+AGG 0.635463 5.3:-20246944 MS.gene069153:intron
GCTGGTGAAGTGACAGAGGT+GGG 0.643936 5.3:-20247426 MS.gene069153:CDS
ATGTCACTCGGACCTTCACA+TGG 0.644647 5.3:-20247617 MS.gene069153:CDS
CAGGGAGAAAGACAATTGGT+GGG 0.647955 5.3:-20246561 MS.gene069153:CDS
TTCATGTCAAAGTTGTGGTG+AGG 0.648184 5.3:-20247337 MS.gene069153:CDS
GTGATCCCAGCACAAAGGAG+AGG 0.651936 5.3:+20247162 None:intergenic
TGCTGGTGAAGTGACAGAGG+TGG 0.668343 5.3:-20247427 MS.gene069153:CDS
TGGAGGCTACTCTGACACCA+TGG 0.673114 5.3:-20247244 MS.gene069153:CDS
CACATGGCTAAGAATGAGTG+GGG 0.673842 5.3:-20247601 MS.gene069153:CDS
GATGGCACTATCACCTATGG+AGG 0.687126 5.3:-20247261 MS.gene069153:CDS
ACCTGGACTGCATATAGGTG+TGG 0.690593 5.3:-20247103 MS.gene069153:CDS
AAAGTGTTCGTCGACAACCA+TGG 0.696527 5.3:+20247227 None:intergenic
ACACTGTGATCCCAGCACAA+AGG 0.700831 5.3:+20247157 None:intergenic
AGTTGCTGGTGAAGTGACAG+AGG 0.727660 5.3:-20247430 MS.gene069153:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATCTATAAAAAAAATAATC+AGG + chr5.3:20246823-20246842 None:intergenic 10.0%
!! AAAATAAATCATATGTCAAT+CGG + chr5.3:20246883-20246902 None:intergenic 15.0%
!! GTTGAAAATTAAATAAACAA+TGG + chr5.3:20247199-20247218 None:intergenic 15.0%
!! TTAAAATAACAATTTCACTA+AGG + chr5.3:20246504-20246523 None:intergenic 15.0%
!! TTACTATATAGAAATAGATT+TGG + chr5.3:20247845-20247864 None:intergenic 15.0%
!!! AAATTAGTTTGTTTTTATGA+CGG + chr5.3:20247237-20247256 None:intergenic 15.0%
!!! GACATATGATTTATTTTAAT+TGG - chr5.3:20246886-20246905 MS.gene069153:intron 15.0%
!! AAAACATCTCATAAATGATA+GGG - chr5.3:20246642-20246661 MS.gene069153:intron 20.0%
!! AAATAAATCATATGTCAATC+GGG + chr5.3:20246882-20246901 None:intergenic 20.0%
!! AAGTTAAAAGATTTAAAGAC+AGG - chr5.3:20246716-20246735 MS.gene069153:CDS 20.0%
!! AATGTTGTTCAATATGTTAT+AGG - chr5.3:20247904-20247923 MS.gene069153:intron 20.0%
!! AGTTAAAAGATTTAAAGACA+GGG - chr5.3:20246717-20246736 MS.gene069153:CDS 20.0%
!! ATATACATCACATTAATTGT+TGG - chr5.3:20248140-20248159 MS.gene069153:intron 20.0%
!! TTGAACAACATTAGAATTAA+AGG + chr5.3:20247896-20247915 None:intergenic 20.0%
!!! AATATTTTTCATTCCTTTTC+AGG - chr5.3:20247037-20247056 MS.gene069153:CDS 20.0%
!!! ATATTTTTCATTCCTTTTCA+GGG - chr5.3:20247038-20247057 MS.gene069153:CDS 20.0%
! AATATGTTATAGGCTGTAAT+GGG - chr5.3:20247914-20247933 MS.gene069153:intron 25.0%
! AGTCATTAAAAGCGAATTAT+TGG + chr5.3:20247812-20247831 None:intergenic 25.0%
! CAACTTGATTGACATTAAAA+GGG + chr5.3:20246566-20246585 None:intergenic 25.0%
! CTAATCCAAAATGTTTCATT+GGG + chr5.3:20247597-20247616 None:intergenic 25.0%
! GAAAACATCTCATAAATGAT+AGG - chr5.3:20246641-20246660 MS.gene069153:intron 25.0%
! TCTAATCCAAAATGTTTCAT+TGG + chr5.3:20247598-20247617 None:intergenic 25.0%
!!! GAATAATGGTAAACTTGTTA+TGG - chr5.3:20248006-20248025 MS.gene069153:intron 25.0%
!!! TATCGATTTGTTATTGACAT+TGG - chr5.3:20248324-20248343 MS.gene069153:CDS 25.0%
AAGGTGAAAAACTTCAAAGT+TGG - chr5.3:20247325-20247344 MS.gene069153:CDS 30.0%
CAATATGTTATAGGCTGTAA+TGG - chr5.3:20247913-20247932 MS.gene069153:intron 30.0%
CCAACTTGATTGACATTAAA+AGG + chr5.3:20246567-20246586 None:intergenic 30.0%
CTTGGTCTATTGAAGAATAA+TGG - chr5.3:20247992-20248011 MS.gene069153:intron 30.0%
GGATTATGTAAATACTGCAA+TGG - chr5.3:20248275-20248294 MS.gene069153:CDS 30.0%
TACATCACAAATCAACATTC+AGG + chr5.3:20246796-20246815 None:intergenic 30.0%
TACTTCTCCTAACAAAAAGA+AGG - chr5.3:20247717-20247736 MS.gene069153:intron 30.0%
! CCAGTATTTTCTTTGATTGT+GGG - chr5.3:20248058-20248077 MS.gene069153:intron 30.0%
! CCTTTTAATGTCAATCAAGT+TGG - chr5.3:20246564-20246583 MS.gene069153:intron 30.0%
! TATAGCCCAATGAAACATTT+TGG - chr5.3:20247589-20247608 MS.gene069153:CDS 30.0%
! TCCAGTATTTTCTTTGATTG+TGG - chr5.3:20248057-20248076 MS.gene069153:intron 30.0%
! TCTCTTATGTTTTCTCAAAC+TGG + chr5.3:20246753-20246772 None:intergenic 30.0%
!! AATGGTAAACTTGTTATGGT+TGG - chr5.3:20248010-20248029 MS.gene069153:intron 30.0%
!! GCATTCAAAGTACTATTTTG+CGG - chr5.3:20247081-20247100 MS.gene069153:CDS 30.0%
!!! ATTTTAATTGGCTCCTTGTA+TGG - chr5.3:20246898-20246917 MS.gene069153:intron 30.0%
!!! CAATCAAGTTGGTTTTCAAT+TGG - chr5.3:20246575-20246594 MS.gene069153:intron 30.0%
AAAACTTCAAAGTTGGAGAC+AGG - chr5.3:20247332-20247351 MS.gene069153:CDS 35.0%
AAACTTCAAAGTTGGAGACA+GGG - chr5.3:20247333-20247352 MS.gene069153:CDS 35.0%
ACATCACATTAATTGTTGGC+AGG - chr5.3:20248144-20248163 MS.gene069153:intron 35.0%
ACTGGATCATCTCTAATGAA+CGG - chr5.3:20246665-20246684 MS.gene069153:intron 35.0%
ATCTCATAAATGATAGGGAC+TGG - chr5.3:20246647-20246666 MS.gene069153:intron 35.0%
CATCACATTAATTGTTGGCA+GGG - chr5.3:20248145-20248164 MS.gene069153:intron 35.0%
CCCACAATCAAAGAAAATAC+TGG + chr5.3:20248061-20248080 None:intergenic 35.0%
GAAAATACTGGAAACTCCAA+AGG + chr5.3:20248049-20248068 None:intergenic 35.0%
GCAGTATTTACATAATCCAC+TGG + chr5.3:20248273-20248292 None:intergenic 35.0%
TACAGTTCCAAGAATGTTGA+TGG - chr5.3:20247445-20247464 MS.gene069153:intron 35.0%
TACCTTTACACCAAAAGCTT+TGG + chr5.3:20247686-20247705 None:intergenic 35.0%
TAGAGAACTTGAAAGGAGAA+AGG + chr5.3:20246449-20246468 None:intergenic 35.0%
TGACATTGGAAACACAATGA+AGG - chr5.3:20248338-20248357 MS.gene069153:CDS 35.0%
TTCTTCAATAGACCAAGCAA+AGG + chr5.3:20247989-20248008 None:intergenic 35.0%
! ATGGCTTCCTTCTTTTTGTT+AGG + chr5.3:20247727-20247746 None:intergenic 35.0%
! ATTTTGCGGTATATGTCACT+CGG - chr5.3:20247095-20247114 MS.gene069153:CDS 35.0%
! TACCTTCTAGAGAACTTGAA+AGG + chr5.3:20246456-20246475 None:intergenic 35.0%
!! ATTGATTTTGCAGCCAAACA+TGG - chr5.3:20248222-20248241 MS.gene069153:intron 35.0%
!! CATTTTGGATTAGACAAACC+TGG - chr5.3:20247604-20247623 MS.gene069153:CDS 35.0%
AAGGAAGCCATTGAACACTT+AGG - chr5.3:20247736-20247755 MS.gene069153:intron 40.0%
ACTCATTCTTAGCCATGTGA+AGG + chr5.3:20247122-20247141 None:intergenic 40.0%
ACTGGAACAACCTCAATATC+AGG + chr5.3:20248255-20248274 None:intergenic 40.0%
AGGCTGCATGAATACATGAA+TGG + chr5.3:20246484-20246503 None:intergenic 40.0%
ATCCCAAAAAAGCTTTCGGA+TGG - chr5.3:20246400-20246419 MS.gene069153:CDS 40.0%
CACCACAACTTTGACATGAA+TGG + chr5.3:20247387-20247406 None:intergenic 40.0%
GAGCATCCCAAAAAAGCTTT+CGG - chr5.3:20246396-20246415 MS.gene069153:CDS 40.0%
GATAGTGCCATCAACATTCT+TGG + chr5.3:20247455-20247474 None:intergenic 40.0%
TCACATGGCTAAGAATGAGT+GGG - chr5.3:20247122-20247141 MS.gene069153:CDS 40.0%
TCCCAAAAAAGCTTTCGGAT+GGG - chr5.3:20246401-20246420 MS.gene069153:CDS 40.0%
TGATATTGAGGTTGTTCCAG+TGG - chr5.3:20248254-20248273 MS.gene069153:intron 40.0%
TGCCATTCATGTCAAAGTTG+TGG - chr5.3:20247382-20247401 MS.gene069153:CDS 40.0%
TGGCGTAAAACCTGATATTG+AGG - chr5.3:20248242-20248261 MS.gene069153:intron 40.0%
TGTTGAAGACATGAAGTTGC+TGG - chr5.3:20247280-20247299 MS.gene069153:CDS 40.0%
TTCACATGGCTAAGAATGAG+TGG - chr5.3:20247121-20247140 MS.gene069153:CDS 40.0%
TTCATGTCAAAGTTGTGGTG+AGG - chr5.3:20247387-20247406 MS.gene069153:CDS 40.0%
TTGACATTAAAAGGGACGCA+AGG + chr5.3:20246558-20246577 None:intergenic 40.0%
! ACAATTGGTGGGAGTATGAT+TGG - chr5.3:20248174-20248193 MS.gene069153:intron 40.0%
! ATCAGGTTTTACGCCATGTT+TGG + chr5.3:20248238-20248257 None:intergenic 40.0%
! GTGATAAGGCATCAGGAATA+CGG + chr5.3:20247528-20247547 None:intergenic 40.0%
! TTTGTGATGTTAGAGTACCC+AGG + chr5.3:20247171-20247190 None:intergenic 40.0%
!! ATTCCTTTTCAGGGAAACAG+GGG - chr5.3:20247047-20247066 MS.gene069153:CDS 40.0%
!! CATTCCTTTTCAGGGAAACA+GGG - chr5.3:20247046-20247065 MS.gene069153:CDS 40.0%
!! CTCCTTTCAAGTTCTCTAGA+AGG - chr5.3:20246451-20246470 MS.gene069153:CDS 40.0%
!! TCATTCCTTTTCAGGGAAAC+AGG - chr5.3:20247045-20247064 MS.gene069153:CDS 40.0%
!! TCTTGGTGGATTAGGTCATA+TGG - chr5.3:20247648-20247667 MS.gene069153:CDS 40.0%
ACTACTTACCCAGTAGTTCC+TGG - chr5.3:20247150-20247169 MS.gene069153:CDS 45.0%
AGCAGCAGCATCAAGTGATA+AGG + chr5.3:20247542-20247561 None:intergenic 45.0%
CACATGGCTAAGAATGAGTG+GGG - chr5.3:20247123-20247142 MS.gene069153:CDS 45.0%
CAGGGAGAAAGACAATTGGT+GGG - chr5.3:20248163-20248182 MS.gene069153:intron 45.0%
CATCCTCTAATGCCTTTGCT+TGG - chr5.3:20247974-20247993 MS.gene069153:intron 45.0%
CTACTTACCCAGTAGTTCCT+GGG - chr5.3:20247151-20247170 MS.gene069153:CDS 45.0%
GACAAACCTGGACTGCATAT+AGG - chr5.3:20247616-20247635 MS.gene069153:CDS 45.0%
GAGTATGATTGGAGGGATGA+AGG - chr5.3:20248185-20248204 MS.gene069153:intron 45.0%
GTCAGCTCCTAAGTGTTCAA+TGG + chr5.3:20247746-20247765 None:intergenic 45.0%
TATTGGTACGCACCTTCATC+TGG + chr5.3:20247795-20247814 None:intergenic 45.0%
TGGCTCCTTGTATGGTAAGA+GGG - chr5.3:20246906-20246925 MS.gene069153:intron 45.0%
TTCAAAGTTGGAGACAGGGT+TGG - chr5.3:20247337-20247356 MS.gene069153:CDS 45.0%
TTGGCAGGGAGAAAGACAAT+TGG - chr5.3:20248159-20248178 MS.gene069153:intron 45.0%
TTGGCTCCTTGTATGGTAAG+AGG - chr5.3:20246905-20246924 MS.gene069153:intron 45.0%
! AAAGTGTTCGTCGACAACCA+TGG + chr5.3:20247500-20247519 None:intergenic 45.0%
! ATGCTCCGTTTCTGGATTTG+AGG + chr5.3:20246382-20246401 None:intergenic 45.0%
! ATTGGTGGGAGTATGATTGG+AGG - chr5.3:20248177-20248196 MS.gene069153:intron 45.0%
! CTTAGGAGCTGACTCATTTG+TGG - chr5.3:20247753-20247772 MS.gene069153:intron 45.0%
! GCATCAAGTGATAAGGCATC+AGG + chr5.3:20247535-20247554 None:intergenic 45.0%
! GTGAAGTTCGCCAAAGCTTT+TGG - chr5.3:20247673-20247692 MS.gene069153:intron 45.0%
! TAGAGTACCCAGGAACTACT+GGG + chr5.3:20247161-20247180 None:intergenic 45.0%
! TTAGAGTACCCAGGAACTAC+TGG + chr5.3:20247162-20247181 None:intergenic 45.0%
! TTGGTGGGAGTATGATTGGA+GGG - chr5.3:20248178-20248197 MS.gene069153:intron 45.0%
!! AGACCAAGCAAAGGCATTAG+AGG + chr5.3:20247980-20247999 None:intergenic 45.0%
!! CAAGCAAAGGCATTAGAGGA+TGG + chr5.3:20247976-20247995 None:intergenic 45.0%
!! CATATAGGTGTGGTTGGTCT+TGG - chr5.3:20247631-20247650 MS.gene069153:CDS 45.0%
!! CGCCAAAGCTTTTGGTGTAA+AGG - chr5.3:20247681-20247700 MS.gene069153:intron 45.0%
!! CGGAGAGTGCTGTGAAATTT+AGG - chr5.3:20246685-20246704 MS.gene069153:CDS 45.0%
!! GTGCTGACTAGTGATAATGC+AGG - chr5.3:20246928-20246947 MS.gene069153:intron 45.0%
!! GTTGATGGCACTATCACCTA+TGG - chr5.3:20247460-20247479 MS.gene069153:intron 45.0%
!! TAGAGGATGGAGTGCTGAAA+CGG + chr5.3:20247963-20247982 None:intergenic 45.0%
!!! GCCCATCCGAAAGCTTTTTT+GGG + chr5.3:20246405-20246424 None:intergenic 45.0%
!!! TGCCCATCCGAAAGCTTTTT+TGG + chr5.3:20246406-20246425 None:intergenic 45.0%
!!! TTTTTGGGATGCTCCGTTTC+TGG + chr5.3:20246390-20246409 None:intergenic 45.0%
ACACTGTGATCCCAGCACAA+AGG + chr5.3:20247570-20247589 None:intergenic 50.0%
ACCACACCTATATGCAGTCC+AGG + chr5.3:20247625-20247644 None:intergenic 50.0%
ACCTGGACTGCATATAGGTG+TGG - chr5.3:20247621-20247640 MS.gene069153:CDS 50.0%
AGTTGCTGGTGAAGTGACAG+AGG - chr5.3:20247294-20247313 MS.gene069153:CDS 50.0%
ATGTCACTCGGACCTTCACA+TGG - chr5.3:20247107-20247126 MS.gene069153:CDS 50.0%
CAGCACCCTCTTACCATACA+AGG + chr5.3:20246914-20246933 None:intergenic 50.0%
GATGGCACTATCACCTATGG+AGG - chr5.3:20247463-20247482 MS.gene069153:intron 50.0%
GCACATCACTCGCGAGAAAT+AGG - chr5.3:20246983-20247002 MS.gene069153:CDS 50.0%
GCAGGGAGAAAGACAATTGG+TGG - chr5.3:20248162-20248181 MS.gene069153:intron 50.0%
GGAAACTCCAAAGGCTTCTC+TGG + chr5.3:20248040-20248059 None:intergenic 50.0%
GGACTGCATATAGGTGTGGT+TGG - chr5.3:20247625-20247644 MS.gene069153:CDS 50.0%
TCGCGAACAAGACCAGATGA+AGG - chr5.3:20247780-20247799 MS.gene069153:intron 50.0%
TCTCCCCTGTTTCCCTGAAA+AGG + chr5.3:20247053-20247072 None:intergenic 50.0%
TTTCAGGGAAACAGGGGAGA+AGG - chr5.3:20247053-20247072 MS.gene069153:CDS 50.0%
!! ATAGGTGTGGTTGGTCTTGG+TGG - chr5.3:20247634-20247653 MS.gene069153:CDS 50.0%
!! GCTGTAATGGGCACGTTTGA+TGG - chr5.3:20247926-20247945 MS.gene069153:intron 50.0%
!! GTGGTTGGTCTTGGTGGATT+AGG - chr5.3:20247640-20247659 MS.gene069153:CDS 50.0%
AAGCTTTCGGATGGGCAGCT+AGG - chr5.3:20246409-20246428 MS.gene069153:CDS 55.0%
AGCTTTCGGATGGGCAGCTA+GGG - chr5.3:20246410-20246429 MS.gene069153:CDS 55.0%
AGTGACAGAGGTGGGAAGCA+AGG - chr5.3:20247306-20247325 MS.gene069153:CDS 55.0%
CTGCTCCTCTCCTTTGTGCT+GGG - chr5.3:20247557-20247576 MS.gene069153:intron 55.0%
GCTGGTGAAGTGACAGAGGT+GGG - chr5.3:20247298-20247317 MS.gene069153:CDS 55.0%
GGAAACAGGGGAGAAGGATG+TGG - chr5.3:20247059-20247078 MS.gene069153:CDS 55.0%
GGTGTCAGAGTAGCCTCCAT+AGG + chr5.3:20247479-20247498 None:intergenic 55.0%
GTGATCCCAGCACAAAGGAG+AGG + chr5.3:20247565-20247584 None:intergenic 55.0%
TGCTGGTGAAGTGACAGAGG+TGG - chr5.3:20247297-20247316 MS.gene069153:CDS 55.0%
TGGAGCTCCAGAGAAGCCTT+TGG - chr5.3:20248030-20248049 MS.gene069153:intron 55.0%
TGGAGGCTACTCTGACACCA+TGG - chr5.3:20247480-20247499 MS.gene069153:intron 55.0%
!! AGTTGGAGACAGGGTTGGTG+TGG - chr5.3:20247342-20247361 MS.gene069153:CDS 55.0%
!! GTTGGAGACAGGGTTGGTGT+GGG - chr5.3:20247343-20247362 MS.gene069153:CDS 55.0%
!! TTGGAGACAGGGTTGGTGTG+GGG - chr5.3:20247344-20247363 MS.gene069153:CDS 55.0%
GCTGCTCCTCTCCTTTGTGC+TGG - chr5.3:20247556-20247575 MS.gene069153:intron 60.0%
GTTGGTGTGGGGTGCATGGT+TGG - chr5.3:20247355-20247374 MS.gene069153:CDS 60.0%
TGGGCAGCTAGGGACACATC+TGG - chr5.3:20246420-20246439 MS.gene069153:CDS 60.0%
!! CAGGGTTGGTGTGGGGTGCA+TGG - chr5.3:20247351-20247370 MS.gene069153:CDS 65.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 20246375 20248371 20246375 ID=MS.gene069153
chr5.3 mRNA 20246375 20248371 20246375 ID=MS.gene069153.t1;Parent=MS.gene069153
chr5.3 exon 20248274 20248371 20248274 ID=MS.gene069153.t1.exon1;Parent=MS.gene069153.t1
chr5.3 CDS 20248274 20248371 20248274 ID=cds.MS.gene069153.t1;Parent=MS.gene069153.t1
chr5.3 exon 20247574 20247687 20247574 ID=MS.gene069153.t1.exon2;Parent=MS.gene069153.t1
chr5.3 CDS 20247574 20247687 20247574 ID=cds.MS.gene069153.t1;Parent=MS.gene069153.t1
chr5.3 exon 20246945 20247458 20246945 ID=MS.gene069153.t1.exon3;Parent=MS.gene069153.t1
chr5.3 CDS 20246945 20247458 20246945 ID=cds.MS.gene069153.t1;Parent=MS.gene069153.t1
chr5.3 exon 20246667 20246820 20246667 ID=MS.gene069153.t1.exon4;Parent=MS.gene069153.t1
chr5.3 CDS 20246667 20246820 20246667 ID=cds.MS.gene069153.t1;Parent=MS.gene069153.t1
chr5.3 exon 20246375 20246580 20246375 ID=MS.gene069153.t1.exon5;Parent=MS.gene069153.t1
chr5.3 CDS 20246375 20246580 20246375 ID=cds.MS.gene069153.t1;Parent=MS.gene069153.t1
Gene Sequence

>MS.gene069153

ATGGCCTCAAATCCAGAAACGGAGCATCCCAAAAAAGCTTTCGGATGGGCAGCTAGGGACACATCTGGAGTCCTTTCTCCTTTCAAGTTCTCTAGAAGGTATGTAACCATTCATGTATTCATGCAGCCTTAGTGAAATTGTTATTTTAACAACAATAAAAGCTACATTTTATTACATTATCCTTGCGTCCCTTTTAATGTCAATCAAGTTGGTTTTCAATTGGTATGATGATTTATGTTGCTTGATTGTTGAGTGATAAAATCAAAGAAAACATCTCATAAATGATAGGGACTGGATCATCTCTAATGAACGGAGAGTGCTGTGAAATTTAGGTCATAAAAAAGTTAAAAGATTTAAAGACAGGGTATGAATTGTCCAGTTTGAGAAAACATAAGAGATTTTCATTGTAGAGATTCATCCTGAATGTTGATTTGTGATGTAAAATCCTGATTATTTTTTTTATAGATAATGCTAAAGATCGAGAAGCATTGTTATAGCGAAAATCCCGATTGACATATGATTTATTTTAATTGGCTCCTTGTATGGTAAGAGGGTGCTGACTAGTGATAATGCAGGTGCTATTTGTGTCGTAATGCTAATTAACATGAGCACATCACTCGCGAGAAATAGGACTGCTCTTTTTTATATATATTTAAATCGATAATATTTTTCATTCCTTTTCAGGGAAACAGGGGAGAAGGATGTGGCATTCAAAGTACTATTTTGCGGTATATGTCACTCGGACCTTCACATGGCTAAGAATGAGTGGGGCAGTACTACTTACCCAGTAGTTCCTGGGTACTCTAACATCACAAAACAAACCATTGTTTATTTAATTTTCAACGAATTAAACATTACTCCGTCATAAAAACAAACTAATTTTTTTGTTTCTTTCTCATCTCATTTGTTGAAGACATGAAGTTGCTGGTGAAGTGACAGAGGTGGGAAGCAAGGTGAAAAACTTCAAAGTTGGAGACAGGGTTGGTGTGGGGTGCATGGTTGGATCTTGCCATTCATGTCAAAGTTGTGGTGAGGATCTCGAAAATTACTGCTCAAAGATGATTCAGACATACAGTTCCAAGAATGTTGATGGCACTATCACCTATGGAGGCTACTCTGACACCATGGTTGTCGACGAACACTTTGTTGTCCGTATTCCTGATGCCTTATCACTTGATGCTGCTGCTCCTCTCCTTTGTGCTGGGATCACAGTGTATAGCCCAATGAAACATTTTGGATTAGACAAACCTGGACTGCATATAGGTGTGGTTGGTCTTGGTGGATTAGGTCATATGGCTGTGAAGTTCGCCAAAGCTTTTGGTGTAAAGGTAACTGTAGTAAGTACTTCTCCTAACAAAAAGAAGGAAGCCATTGAACACTTAGGAGCTGACTCATTTGTGGTTAGTCGCGAACAAGACCAGATGAAGGTGCGTACCAATAATTCGCTTTTAATGACTAATTTAGAAACCAAATCTATTTCTATATAGTAATCGATTTATCGACGATCTAAAAAATTGACCTTTAATTCTAATGTTGTTCAATATGTTATAGGCTGTAATGGGCACGTTTGATGGAATCATCGACACCGTTTCAGCACTCCATCCTCTAATGCCTTTGCTTGGTCTATTGAAGAATAATGGTAAACTTGTTATGGTTGGAGCTCCAGAGAAGCCTTTGGAGTTTCCAGTATTTTCTTTGATTGTGGGTAAGCATATGATAAAAATTCTATACAGACTATGTTATATTAAAATATGAAAATTGTTAAATATACATCACATTAATTGTTGGCAGGGAGAAAGACAATTGGTGGGAGTATGATTGGAGGGATGAAGGAAACACAAGAAATGATTGATTTTGCAGCCAAACATGGCGTAAAACCTGATATTGAGGTTGTTCCAGTGGATTATGTAAATACTGCAATGGAGCGCTTACTCAAAGCTGATGTGAAATATCGATTTGTTATTGACATTGGAAACACAATGAAGGCAAGCTCTTAA
Protein sequence

>MS.gene069153.t1

MASNPETEHPKKAFGWAARDTSGVLSPFKFSRRETGEKDVAFKVLFCGICHSDLHMAKNEWGSTTYPVVPGHEVAGEVTEVGSKVKNFKVGDRVGVGCMVGSCHSCQSCGEDLENYCSKMIQTYSSKNVDGTITYGGYSDTMVVDEHFVVRIPDALSLDAAAPLLCAGITVYSPMKHFGLDKPGLHIGVVGLGGLGHMAVKFAKAFGVKVTVVSTSPNKKKEAIEHLGADSFVVSREQDQMKAVMGTFDGIIDTVSALHPLMPLLGLLKNNGKLVMVGAPEKPLEFPVFSLIVGRKTIGGSMIGGMKETQEMIDFAAKHGVKPDIEVVPVDYVNTAMERLLKADVKYRFVIDIGNTMKASS