AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene027818

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027818.t1 MTR_5g031310 96.409 362 13 0 1 362 1 362 0.0 721
MS.gene027818.t1 MTR_5g031305 93.923 362 22 0 1 362 1 362 0.0 689
MS.gene027818.t1 MTR_5g031360 87.151 358 46 0 5 362 6 363 0.0 631
MS.gene027818.t1 MTR_5g031300 84.488 361 56 0 1 361 1 361 0.0 629
MS.gene027818.t1 MTR_5g031230 83.102 361 60 1 1 361 1 360 0.0 603
MS.gene027818.t1 MTR_4g115300 82.768 354 61 0 8 361 7 360 0.0 599
MS.gene027818.t1 MTR_5g031390 83.051 354 60 0 8 361 7 360 0.0 594
MS.gene027818.t1 MTR_5g031250 80.332 361 70 1 1 361 1 360 0.0 581
MS.gene027818.t1 MTR_5g031210 79.661 354 72 0 8 361 7 360 0.0 565
MS.gene027818.t1 MTR_5g031190 76.204 353 84 0 9 361 8 360 0.0 556
MS.gene027818.t1 MTR_5g031180 80.226 354 70 0 8 361 7 360 0.0 551
MS.gene027818.t1 MTR_8g068190 79.096 354 74 0 8 361 7 360 0.0 544
MS.gene027818.t1 MTR_8g092630 61.326 362 134 2 2 358 53 413 1.18e-151 434
MS.gene027818.t1 MTR_5g031430 60.000 345 138 0 15 359 14 358 7.79e-145 414
MS.gene027818.t1 MTR_5g031250 81.818 242 43 1 1 242 1 241 1.72e-140 399
MS.gene027818.t1 MTR_1g025950 57.184 348 148 1 10 356 8 355 2.60e-133 385
MS.gene027818.t1 MTR_1g107425 51.020 343 168 0 12 354 9 351 3.12e-121 354
MS.gene027818.t1 MTR_2g009810 53.602 347 157 3 15 359 62 406 6.19e-117 345
MS.gene027818.t1 MTR_1g107425 51.211 289 140 1 12 299 9 297 2.72e-96 288
MS.gene027818.t1 MTR_2g009820 52.155 232 108 2 131 359 23 254 8.73e-64 204
MS.gene027818.t1 MTR_5g031290 76.522 115 27 0 248 362 80 194 8.63e-55 179
MS.gene027818.t1 MTR_5g031290 82.716 81 12 2 1 80 1 80 7.74e-41 142
MS.gene027818.t1 MTR_0001s0660 28.289 304 190 10 39 319 35 333 4.48e-21 94.4
MS.gene027818.t1 MTR_8g095220 26.599 297 186 8 11 279 51 343 4.60e-21 94.0
MS.gene027818.t1 MTR_8g095220 28.125 256 157 7 11 243 51 302 9.41e-21 92.0
MS.gene027818.t1 MTR_6g465010 29.108 213 135 7 26 234 13 213 1.87e-16 80.1
MS.gene027818.t1 MTR_3g089977 24.339 378 242 13 12 355 12 379 2.77e-16 79.7
MS.gene027818.t1 MTR_6g466320 28.638 213 136 6 26 234 29 229 2.79e-16 79.7
MS.gene027818.t1 MTR_6g465010 28.638 213 136 6 26 234 29 229 2.79e-16 79.7
MS.gene027818.t1 MTR_3g089940 24.728 368 234 12 12 345 12 370 5.84e-16 79.0
MS.gene027818.t1 MTR_7g068320 26.667 345 199 13 40 341 48 381 7.37e-16 78.6
MS.gene027818.t1 MTR_3g089940 24.274 379 244 12 1 345 1 370 1.09e-15 77.8
MS.gene027818.t1 MTR_3g089940 25.856 263 168 7 12 254 12 267 5.49e-15 75.5
MS.gene027818.t1 MTR_3g089940 25.182 274 178 7 1 254 1 267 8.67e-15 75.1
MS.gene027818.t1 MTR_3g089970 24.317 366 234 12 14 345 15 371 1.31e-14 74.7
MS.gene027818.t1 MTR_5g045710 23.881 335 224 9 39 345 35 366 3.08e-14 73.6
MS.gene027818.t1 MTR_7g068330 26.377 345 199 14 40 341 41 373 7.46e-14 72.4
MS.gene027818.t1 MTR_1g069430 26.822 343 209 14 1 313 1 331 7.46e-13 69.3
MS.gene027818.t1 MTR_7g099040 24.930 357 225 13 2 328 4 347 1.96e-12 68.2
MS.gene027818.t1 MTR_5g031380 64.103 39 14 0 81 119 19 57 2.25e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027818.t1 AT4G37980 71.989 357 98 1 6 362 3 357 6.73e-177 495
MS.gene027818.t1 AT4G37990 71.148 357 101 1 6 362 3 357 5.96e-173 485
MS.gene027818.t1 AT4G37970 63.173 353 130 0 9 361 9 361 9.49e-164 462
MS.gene027818.t1 AT4G39330 62.500 352 132 0 9 360 8 359 2.79e-162 458
MS.gene027818.t1 AT2G21730 60.571 350 136 2 13 360 6 355 3.97e-150 428
MS.gene027818.t1 AT2G21890 59.885 349 139 1 13 360 6 354 5.75e-150 428
MS.gene027818.t1 AT4G37980 70.805 298 85 1 6 303 3 298 1.78e-136 390
MS.gene027818.t1 AT4G39330 62.162 296 112 0 9 304 8 303 2.90e-131 378
MS.gene027818.t1 AT4G37970 61.724 290 111 0 9 298 9 298 4.69e-127 367
MS.gene027818.t1 AT3G19450 52.312 346 165 0 12 357 9 354 5.27e-115 338
MS.gene027818.t1 AT1G72680 50.437 343 169 1 17 359 14 355 1.08e-113 335
MS.gene027818.t1 AT4G34230 50.575 348 172 0 7 354 3 350 2.31e-106 316
MS.gene027818.t1 AT4G34230 50.287 348 173 0 7 354 3 350 2.06e-105 314
MS.gene027818.t1 AT4G37970 60.924 238 93 0 9 246 9 246 2.79e-101 300
MS.gene027818.t1 AT4G37970 61.277 235 91 0 9 243 9 243 2.09e-100 297
MS.gene027818.t1 AT5G63620 26.207 290 186 6 16 279 58 345 9.92e-23 99.0
MS.gene027818.t1 AT5G63620 26.207 290 186 6 16 279 58 345 1.64e-22 98.6
MS.gene027818.t1 AT5G63620 27.711 249 157 5 16 243 58 304 8.48e-22 95.1
MS.gene027818.t1 AT5G43940 26.901 342 210 12 1 313 1 331 4.13e-17 82.4
MS.gene027818.t1 AT1G22430 25.868 317 203 10 40 328 42 354 5.76e-17 82.0
MS.gene027818.t1 AT1G22430 25.868 317 203 10 40 328 42 354 5.76e-17 82.0
MS.gene027818.t1 AT5G43940 27.063 303 185 9 40 313 48 343 9.91e-17 81.3
MS.gene027818.t1 AT5G51970 25.490 255 169 6 37 279 40 285 5.46e-16 79.0
MS.gene027818.t1 AT5G51970 25.490 255 169 6 37 279 40 285 5.46e-16 79.0
MS.gene027818.t1 AT1G32780 27.072 362 217 15 40 359 37 393 6.69e-16 79.0
MS.gene027818.t1 AT1G22430 26.000 200 129 5 40 222 42 239 4.04e-13 69.7
MS.gene027818.t1 AT1G22430 26.000 200 129 5 40 222 42 239 6.00e-13 68.9
MS.gene027818.t1 AT1G22430 26.000 200 129 5 40 222 42 239 6.00e-13 68.9
MS.gene027818.t1 AT4G22110 25.075 335 215 13 40 344 43 371 7.04e-13 69.7
MS.gene027818.t1 AT4G22110 25.075 335 215 13 40 344 43 371 7.04e-13 69.7
MS.gene027818.t1 AT1G77120 22.597 385 261 13 1 355 1 378 8.49e-13 69.3
MS.gene027818.t1 AT5G42250 21.114 341 236 9 40 353 45 379 6.53e-11 63.5

Find 92 sgRNAs with CRISPR-Local

Find 117 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAGTAGCTGGAAGTTTAAT+TGG 0.186001 5.2:-21640337 MS.gene027818:CDS
TATAGTCCTCTTCGACATTT+TGG 0.197168 5.2:-21640922 MS.gene027818:CDS
AAACCAATCAATGGTAAAAT+AGG 0.229602 5.2:+21640566 None:intergenic
TATCGATTCGTGATTGATAT+TGG 0.245698 5.2:-21640187 MS.gene027818:CDS
GTGAAATTCGCCAAAGCTTT+TGG 0.268679 5.2:-21640838 MS.gene027818:CDS
ATTGGTTTATTGAAATCTAA+TGG 0.271827 5.2:-21640551 MS.gene027818:CDS
TCCTGCAATGTTTAAGGGCT+TGG 0.287864 5.2:+21640151 None:intergenic
TCTTGGTGGACTTGGTCATA+TGG 0.309264 5.2:-21640863 MS.gene027818:CDS
ACCACATGTGACTGTTTGTT+GGG 0.314826 5.2:+21641083 None:intergenic
CCCATTCATTCTTCATCATA+TGG 0.323377 5.2:+21641434 None:intergenic
CATCAAAACTTTAGGGAAAC+TGG 0.347590 5.2:-21641511 MS.gene027818:intron
GCCCAACCAAAGGCCTTCTT+AGG 0.362894 5.2:+21641723 None:intergenic
CACCACATGTGACTGTTTGT+TGG 0.363970 5.2:+21641082 None:intergenic
CCCTGGAACTAATGGATAGA+TGG 0.366233 5.2:+21641405 None:intergenic
AAGTTTAATTGGAGGGATAA+AGG 0.373525 5.2:-21640326 MS.gene027818:CDS
GTTGTTGGTCTTGGTGGACT+TGG 0.377317 5.2:-21640871 MS.gene027818:CDS
TCCATCTATCCATTAGTTCC+AGG 0.385414 5.2:-21641406 MS.gene027818:CDS
GTTAGGAGATGTGCTAATTA+CGG 0.387790 5.2:+21640804 None:intergenic
AACTTACCTCTTGATGTTGC+CGG 0.388597 5.2:-21640973 MS.gene027818:CDS
TCCTAAGAAGGCCTTTGGTT+GGG 0.390941 5.2:-21641724 MS.gene027818:CDS
ACATCAAGAGGTAAGTTATC+AGG 0.391560 5.2:+21640979 None:intergenic
CGTGATGGCACCGTCACATA+TGG 0.395744 5.2:-21641051 MS.gene027818:CDS
ATGGGAATAACCTCAATATC+AGG 0.396719 5.2:+21640259 None:intergenic
GAGCATCCTAAGAAGGCCTT+TGG 0.400649 5.2:-21641729 MS.gene027818:CDS
ATAGGTGTTGTTGGTCTTGG+TGG 0.407624 5.2:-21640877 MS.gene027818:CDS
GCCGGTCCTCTTCTTTGCGC+TGG 0.408424 5.2:-21640955 MS.gene027818:CDS
CACATTAGCACCAAAAGCTT+TGG 0.409643 5.2:+21640828 None:intergenic
CCCCTTTCAATTTCTCCAGA+AGG 0.415467 5.2:-21641674 MS.gene027818:intron
AATATAGGTGTTGTTGGTCT+TGG 0.416186 5.2:-21640880 MS.gene027818:CDS
TCAAATACCTGCATCTGATC+TGG 0.416400 5.2:+21640724 None:intergenic
AAATGCAGGCATGAACTTGC+GGG 0.418681 5.2:-21641231 MS.gene027818:intron
GAAGCATACCCTGGAACTAA+TGG 0.426302 5.2:+21641397 None:intergenic
AAAGTGGAAAAGTTTAAAGT+TGG 0.429905 5.2:-21641186 MS.gene027818:CDS
TCCATATGATGAAGAATGAA+TGG 0.431554 5.2:-21641435 MS.gene027818:CDS
GTAGGGAGAAAGTTAGTAGC+TGG 0.438913 5.2:-21640349 MS.gene027818:intron
TTTAAAGTTGGAGACAAAGT+AGG 0.443860 5.2:-21641174 MS.gene027818:CDS
GACGGTGCCATCACGATACT+TGG 0.453032 5.2:+21641059 None:intergenic
TGGGCAGCTAGAGATCCTTC+TGG 0.459135 5.2:-21641705 MS.gene027818:CDS
TGAGAAAGATGTAGCATTCA+AGG 0.467351 5.2:-21641488 MS.gene027818:CDS
GACAAACCTGGTATGAATAT+AGG 0.467866 5.2:-21640895 MS.gene027818:CDS
CCATATGATGAAGAATGAAT+GGG 0.478005 5.2:-21641434 MS.gene027818:CDS
CAAAGTAGGTGTGGGATACA+TGG 0.478011 5.2:-21641160 MS.gene027818:CDS
CACATCTCCTAACAAAGAAA+AGG 0.480129 5.2:-21640794 MS.gene027818:CDS
TTCCACTTTGCTTCCTACCT+CGG 0.483575 5.2:+21641200 None:intergenic
CCTCCAAGTAAAGAAAATAC+AGG 0.487127 5.2:+21640485 None:intergenic
GATTTCAATAAACCAATCAA+TGG 0.487235 5.2:+21640557 None:intergenic
CAATGTAAAGCCTGATATTG+AGG 0.489359 5.2:-21640269 MS.gene027818:CDS
TGGAGGTTATTCTGACTCAA+TGG 0.494148 5.2:-21641031 MS.gene027818:CDS
ACCTTCTGGAGAAATTGAAA+GGG 0.500807 5.2:+21641673 None:intergenic
ATCCTAAGAAGGCCTTTGGT+TGG 0.502470 5.2:-21641725 MS.gene027818:CDS
AACCTTCTGGAGAAATTGAA+AGG 0.504412 5.2:+21641672 None:intergenic
GGTATGAATATAGGTGTTGT+TGG 0.507632 5.2:-21640886 MS.gene027818:CDS
AAAGGAAGCAATAGAACACT+TGG 0.509890 5.2:-21640776 MS.gene027818:CDS
AGTGTTAGAGCATCCTAAGA+AGG 0.523098 5.2:-21641736 MS.gene027818:CDS
TGCTGTGTTGACATAATCAA+TGG 0.528228 5.2:+21640240 None:intergenic
TAGCTGGAAGTTTAATTGGA+GGG 0.528518 5.2:-21640333 MS.gene027818:CDS
AGTTGGAGACAAAGTAGGTG+TGG 0.528716 5.2:-21641169 MS.gene027818:CDS
GCTGCAACGGGTACTTTGAA+TGG 0.533300 5.2:-21640617 MS.gene027818:CDS
CCACATGTGACTGTTTGTTG+GGG 0.540150 5.2:+21641084 None:intergenic
GAAAATACAGGAAGCTCTAG+AGG 0.546279 5.2:+21640497 None:intergenic
CCATCTATCCATTAGTTCCA+GGG 0.551121 5.2:-21641405 MS.gene027818:intron
GTAGCTGGAAGTTTAATTGG+AGG 0.559892 5.2:-21640334 MS.gene027818:CDS
GCATTCAAGGTGTTGTATTG+TGG 0.562216 5.2:-21641475 MS.gene027818:CDS
GGAAAGCTTGTAATGGTTGG+TGG 0.563386 5.2:-21640530 MS.gene027818:CDS
TCCAGCGCAAAGAAGAGGAC+CGG 0.564917 5.2:+21640954 None:intergenic
GTGACTCCAGCGCAAAGAAG+AGG 0.566494 5.2:+21640949 None:intergenic
TAAATGCAGGCATGAACTTG+CGG 0.570766 5.2:-21641232 MS.gene027818:intron
ATCTCTAGCTGCCCAACCAA+AGG 0.576625 5.2:+21641713 None:intergenic
GTTGGAGACAAAGTAGGTGT+GGG 0.583586 5.2:-21641168 MS.gene027818:CDS
ATCTAATGGAAAGCTTGTAA+TGG 0.587781 5.2:-21640537 MS.gene027818:CDS
CCGCGATCCAGATCAGATGC+AGG 0.596450 5.2:-21640731 MS.gene027818:intron
GCTTGTAATGGTTGGTGGAG+TGG 0.601696 5.2:-21640525 MS.gene027818:CDS
GCTGTGTTGACATAATCAAT+GGG 0.609776 5.2:+21640241 None:intergenic
AGAGGACCGGCAACATCAAG+AGG 0.610980 5.2:+21640967 None:intergenic
AATGGAAAGCTTGTAATGGT+TGG 0.618001 5.2:-21640533 MS.gene027818:CDS
ACAACACCTATATTCATACC+AGG 0.624332 5.2:+21640889 None:intergenic
TTTGAATGGTATTATTGACA+CGG 0.632246 5.2:-21640603 MS.gene027818:CDS
AAAGGGGAGAGAACACCAGA+AGG 0.635132 5.2:+21641690 None:intergenic
GCGGGTATAGTAACCGAGGT+AGG 0.635211 5.2:-21641213 MS.gene027818:CDS
TCGAGTCCAAAATGTCGAAG+AGG 0.641706 5.2:+21640916 None:intergenic
GATGGCACCGTCACATATGG+AGG 0.644175 5.2:-21641048 MS.gene027818:CDS
TGATTATGTCAACACAGCAG+TGG 0.644987 5.2:-21640236 MS.gene027818:CDS
CCTTCTGGAGAAATTGAAAG+GGG 0.647504 5.2:+21641674 None:intergenic
CCTGCATCTGATCTGGATCG+CGG 0.652791 5.2:+21640731 None:intergenic
AGAATAACCTCCATATGTGA+CGG 0.659885 5.2:+21641041 None:intergenic
CACATGTGACTGTTTGTTGG+GGG 0.661508 5.2:+21641085 None:intergenic
AACCGAGGTAGGAAGCAAAG+TGG 0.665272 5.2:-21641202 MS.gene027818:CDS
CATATGATGAAGAATGAATG+GGG 0.667164 5.2:-21641433 MS.gene027818:CDS
AAGGAAGCAATAGAACACTT+GGG 0.667331 5.2:-21640775 MS.gene027818:CDS
CCCCAACAAACAGTCACATG+TGG 0.678792 5.2:-21641084 MS.gene027818:CDS
TGTGGTGCCAAGTATCGTGA+TGG 0.704207 5.2:-21641066 MS.gene027818:CDS
ACTTGCGGGTATAGTAACCG+AGG 0.756724 5.2:-21641217 MS.gene027818:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGTAAGTATAAAGATATATA+TGG + chr5.2:21640589-21640608 None:intergenic 15.0%
!! ATAAATTTCACATTATTTGT+AGG - chr5.2:21641548-21641567 MS.gene027818:intron 15.0%
!! TAAATTTCACATTATTTGTA+GGG - chr5.2:21641549-21641568 MS.gene027818:intron 15.0%
!! TAAAGTAAAATTGTAGTAGA+AGG + chr5.2:21640549-21640568 None:intergenic 20.0%
!! TGCTTATAATTAATAAATGC+AGG - chr5.2:21640670-21640689 MS.gene027818:intron 20.0%
!!! ATAATGTACCACATTTTAAT+AGG - chr5.2:21641276-21641295 MS.gene027818:intron 20.0%
!!! ATCAACACATCTTTATTTAA+TGG - chr5.2:21640278-21640297 MS.gene027818:CDS 20.0%
!!! ATTGGTTTATTGAAATCTAA+TGG - chr5.2:21641364-21641383 MS.gene027818:intron 20.0%
!!! TCAACACATCTTTATTTAAT+GGG - chr5.2:21640279-21640298 MS.gene027818:CDS 20.0%
!!! TTAGTCTTAAAACTATACTA+TGG + chr5.2:21641477-21641496 None:intergenic 20.0%
! AAACCAATCAATGGTAAAAT+AGG + chr5.2:21641352-21641371 None:intergenic 25.0%
! GATTTCAATAAACCAATCAA+TGG + chr5.2:21641361-21641380 None:intergenic 25.0%
! TTATAATGAAGTAAACCTTC+TGG + chr5.2:21640259-21640278 None:intergenic 25.0%
! TTTGAATGGTATTATTGACA+CGG - chr5.2:21641312-21641331 MS.gene027818:intron 25.0%
!! AAAGTGGAAAAGTTTAAAGT+TGG - chr5.2:21640729-21640748 MS.gene027818:intron 25.0%
!!! TACTTTATTTCTACGTTACT+TGG - chr5.2:21640562-21640581 MS.gene027818:CDS 25.0%
!!! TCAAGAAATGATTGATTTTG+CGG - chr5.2:21641616-21641635 MS.gene027818:intron 25.0%
AAAATTGTAGTAGAAGGAGT+TGG + chr5.2:21640543-21640562 None:intergenic 30.0%
AAGTTTAATTGGAGGGATAA+AGG - chr5.2:21641589-21641608 MS.gene027818:intron 30.0%
ATCTAATGGAAAGCTTGTAA+TGG - chr5.2:21641378-21641397 MS.gene027818:intron 30.0%
CATATGATGAAGAATGAATG+GGG - chr5.2:21640482-21640501 MS.gene027818:intron 30.0%
CCATATGATGAAGAATGAAT+GGG - chr5.2:21640481-21640500 MS.gene027818:intron 30.0%
TCCATATGATGAAGAATGAA+TGG - chr5.2:21640480-21640499 MS.gene027818:intron 30.0%
TTAGTAGCTGGAAGTTTAAT+TGG - chr5.2:21641578-21641597 MS.gene027818:intron 30.0%
! ATTGCTTCCTTTTCTTTGTT+AGG + chr5.2:21641131-21641150 None:intergenic 30.0%
! CATCCTATTTTACCATTGAT+TGG - chr5.2:21641346-21641365 MS.gene027818:intron 30.0%
! CTTCCTGTATTTTCTTTACT+TGG - chr5.2:21641427-21641446 MS.gene027818:CDS 30.0%
! TTTAAAGTTGGAGACAAAGT+AGG - chr5.2:21640741-21640760 MS.gene027818:CDS 30.0%
! TTTGGTGCATCAAAACTTTA+GGG - chr5.2:21640397-21640416 MS.gene027818:intron 30.0%
!! TATCGATTCGTGATTGATAT+TGG - chr5.2:21641728-21641747 MS.gene027818:CDS 30.0%
AAAGGAAGCAATAGAACACT+TGG - chr5.2:21641139-21641158 MS.gene027818:CDS 35.0%
AAGGAAGCAATAGAACACTT+GGG - chr5.2:21641140-21641159 MS.gene027818:CDS 35.0%
AATGGAAAGCTTGTAATGGT+TGG - chr5.2:21641382-21641401 MS.gene027818:intron 35.0%
ACAACACCTATATTCATACC+AGG + chr5.2:21641029-21641048 None:intergenic 35.0%
ACATCAAGAGGTAAGTTATC+AGG + chr5.2:21640939-21640958 None:intergenic 35.0%
AGAATAACCTCCATATGTGA+CGG + chr5.2:21640877-21640896 None:intergenic 35.0%
ATGGGAATAACCTCAATATC+AGG + chr5.2:21641659-21641678 None:intergenic 35.0%
CAATGTAAAGCCTGATATTG+AGG - chr5.2:21641646-21641665 MS.gene027818:intron 35.0%
CACATCTCCTAACAAAGAAA+AGG - chr5.2:21641121-21641140 MS.gene027818:CDS 35.0%
CCCATTCATTCTTCATCATA+TGG + chr5.2:21640484-21640503 None:intergenic 35.0%
CCTCCAAGTAAAGAAAATAC+AGG + chr5.2:21641433-21641452 None:intergenic 35.0%
GACAAACCTGGTATGAATAT+AGG - chr5.2:21641020-21641039 MS.gene027818:CDS 35.0%
GCTACTAATTAAGCCAACTT+TGG - chr5.2:21640379-21640398 MS.gene027818:intron 35.0%
GTTAGGAGATGTGCTAATTA+CGG + chr5.2:21641114-21641133 None:intergenic 35.0%
TAGCTGGAAGTTTAATTGGA+GGG - chr5.2:21641582-21641601 MS.gene027818:intron 35.0%
TGAGAAAGATGTAGCATTCA+AGG - chr5.2:21640427-21640446 MS.gene027818:intron 35.0%
! AACCTTCTGGAGAAATTGAA+AGG + chr5.2:21640246-21640265 None:intergenic 35.0%
! ACCTTCTGGAGAAATTGAAA+GGG + chr5.2:21640245-21640264 None:intergenic 35.0%
! CATCAAAACTTTAGGGAAAC+TGG - chr5.2:21640404-21640423 MS.gene027818:intron 35.0%
! CCTGTATTTTCTTTACTTGG+AGG - chr5.2:21641430-21641449 MS.gene027818:CDS 35.0%
! CTTTGGTGCATCAAAACTTT+AGG - chr5.2:21640396-21640415 MS.gene027818:intron 35.0%
! GCTGTGTTGACATAATCAAT+GGG + chr5.2:21641677-21641696 None:intergenic 35.0%
! GGTATGAATATAGGTGTTGT+TGG - chr5.2:21641029-21641048 MS.gene027818:CDS 35.0%
! TATAGTCCTCTTCGACATTT+TGG - chr5.2:21640993-21641012 MS.gene027818:CDS 35.0%
! TGCTGTGTTGACATAATCAA+TGG + chr5.2:21641678-21641697 None:intergenic 35.0%
!! AAGTTTTGATGCACCAAAGT+TGG + chr5.2:21640395-21640414 None:intergenic 35.0%
!! AATATAGGTGTTGTTGGTCT+TGG - chr5.2:21641035-21641054 MS.gene027818:CDS 35.0%
!! ACATTTTAATAGGCTGCAAC+GGG - chr5.2:21641286-21641305 MS.gene027818:intron 35.0%
!! CACATTTTAATAGGCTGCAA+CGG - chr5.2:21641285-21641304 MS.gene027818:intron 35.0%
AACTTACCTCTTGATGTTGC+CGG - chr5.2:21640942-21640961 MS.gene027818:CDS 40.0%
ACCACATGTGACTGTTTGTT+GGG + chr5.2:21640835-21640854 None:intergenic 40.0%
CACATTAGCACCAAAAGCTT+TGG + chr5.2:21641090-21641109 None:intergenic 40.0%
CCATCTATCCATTAGTTCCA+GGG - chr5.2:21640510-21640529 MS.gene027818:CDS 40.0%
CGTTGCAGCCTATTAAAATG+TGG + chr5.2:21641287-21641306 None:intergenic 40.0%
GTAGCTGGAAGTTTAATTGG+AGG - chr5.2:21641581-21641600 MS.gene027818:intron 40.0%
TAAATGCAGGCATGAACTTG+CGG - chr5.2:21640683-21640702 MS.gene027818:intron 40.0%
TCAAATACCTGCATCTGATC+TGG + chr5.2:21641194-21641213 None:intergenic 40.0%
TCCATCTATCCATTAGTTCC+AGG - chr5.2:21640509-21640528 MS.gene027818:CDS 40.0%
TGATTATGTCAACACAGCAG+TGG - chr5.2:21641679-21641698 MS.gene027818:CDS 40.0%
TGGAGGTTATTCTGACTCAA+TGG - chr5.2:21640884-21640903 MS.gene027818:CDS 40.0%
! AGTGTTAGAGCATCCTAAGA+AGG - chr5.2:21640179-21640198 MS.gene027818:CDS 40.0%
! CCTTCTGGAGAAATTGAAAG+GGG + chr5.2:21640244-21640263 None:intergenic 40.0%
! GAAAATACAGGAAGCTCTAG+AGG + chr5.2:21641421-21641440 None:intergenic 40.0%
! GTGAAATTCGCCAAAGCTTT+TGG - chr5.2:21641077-21641096 MS.gene027818:CDS 40.0%
!! GCATTCAAGGTGTTGTATTG+TGG - chr5.2:21640440-21640459 MS.gene027818:intron 40.0%
AAATGCAGGCATGAACTTGC+GGG - chr5.2:21640684-21640703 MS.gene027818:intron 45.0%
AGTTGGAGACAAAGTAGGTG+TGG - chr5.2:21640746-21640765 MS.gene027818:CDS 45.0%
ATCCTAAGAAGGCCTTTGGT+TGG - chr5.2:21640190-21640209 MS.gene027818:CDS 45.0%
CAAAGTAGGTGTGGGATACA+TGG - chr5.2:21640755-21640774 MS.gene027818:CDS 45.0%
CACCACATGTGACTGTTTGT+TGG + chr5.2:21640836-21640855 None:intergenic 45.0%
CCCCTTTCAATTTCTCCAGA+AGG - chr5.2:21640241-21640260 MS.gene027818:CDS 45.0%
GAAGCATACCCTGGAACTAA+TGG + chr5.2:21640521-21640540 None:intergenic 45.0%
GGAAAGCTTGTAATGGTTGG+TGG - chr5.2:21641385-21641404 MS.gene027818:intron 45.0%
GTAGGGAGAAAGTTAGTAGC+TGG - chr5.2:21641566-21641585 MS.gene027818:intron 45.0%
GTTGGAGACAAAGTAGGTGT+GGG - chr5.2:21640747-21640766 MS.gene027818:CDS 45.0%
TCCTAAGAAGGCCTTTGGTT+GGG - chr5.2:21640191-21640210 MS.gene027818:CDS 45.0%
TCGAGTCCAAAATGTCGAAG+AGG + chr5.2:21641002-21641021 None:intergenic 45.0%
TTCCACTTTGCTTCCTACCT+CGG + chr5.2:21640718-21640737 None:intergenic 45.0%
! CACATGTGACTGTTTGTTGG+GGG + chr5.2:21640833-21640852 None:intergenic 45.0%
! CATTTTGGACTCGACAAACC+TGG - chr5.2:21641008-21641027 MS.gene027818:CDS 45.0%
! CCACATGTGACTGTTTGTTG+GGG + chr5.2:21640834-21640853 None:intergenic 45.0%
! CCCTGGAACTAATGGATAGA+TGG + chr5.2:21640513-21640532 None:intergenic 45.0%
!! ATAGGTGTTGTTGGTCTTGG+TGG - chr5.2:21641038-21641057 MS.gene027818:CDS 45.0%
!! TCTTGGTGGACTTGGTCATA+TGG - chr5.2:21641052-21641071 MS.gene027818:CDS 45.0%
AAAGGGGAGAGAACACCAGA+AGG + chr5.2:21640228-21640247 None:intergenic 50.0%
AACCGAGGTAGGAAGCAAAG+TGG - chr5.2:21640713-21640732 MS.gene027818:intron 50.0%
ACTTGCGGGTATAGTAACCG+AGG - chr5.2:21640698-21640717 MS.gene027818:intron 50.0%
ATCTCTAGCTGCCCAACCAA+AGG + chr5.2:21640205-21640224 None:intergenic 50.0%
CCCCAACAAACAGTCACATG+TGG - chr5.2:21640831-21640850 MS.gene027818:CDS 50.0%
GAGCATCCTAAGAAGGCCTT+TGG - chr5.2:21640186-21640205 MS.gene027818:CDS 50.0%
GCTGCAACGGGTACTTTGAA+TGG - chr5.2:21641298-21641317 MS.gene027818:intron 50.0%
! AAGGAGTTGGAAGCATACCC+TGG + chr5.2:21640530-21640549 None:intergenic 50.0%
! TGTGGTGCCAAGTATCGTGA+TGG - chr5.2:21640849-21640868 MS.gene027818:CDS 50.0%
!! GCTTGTAATGGTTGGTGGAG+TGG - chr5.2:21641390-21641409 MS.gene027818:intron 50.0%
!! GTTGTTGGTCTTGGTGGACT+TGG - chr5.2:21641044-21641063 MS.gene027818:CDS 50.0%
AGAGGACCGGCAACATCAAG+AGG + chr5.2:21640951-21640970 None:intergenic 55.0%
CCTGCATCTGATCTGGATCG+CGG + chr5.2:21641187-21641206 None:intergenic 55.0%
CGTGATGGCACCGTCACATA+TGG - chr5.2:21640864-21640883 MS.gene027818:CDS 55.0%
GATGGCACCGTCACATATGG+AGG - chr5.2:21640867-21640886 MS.gene027818:CDS 55.0%
GCCCAACCAAAGGCCTTCTT+AGG + chr5.2:21640195-21640214 None:intergenic 55.0%
GCGGGTATAGTAACCGAGGT+AGG - chr5.2:21640702-21640721 MS.gene027818:intron 55.0%
GTGACTCCAGCGCAAAGAAG+AGG + chr5.2:21640969-21640988 None:intergenic 55.0%
TCCAGCGCAAAGAAGAGGAC+CGG + chr5.2:21640964-21640983 None:intergenic 55.0%
TGGGCAGCTAGAGATCCTTC+TGG - chr5.2:21640210-21640229 MS.gene027818:CDS 55.0%
!! GACGGTGCCATCACGATACT+TGG + chr5.2:21640859-21640878 None:intergenic 55.0%
CCGCGATCCAGATCAGATGC+AGG - chr5.2:21641184-21641203 MS.gene027818:CDS 60.0%
GCCGGTCCTCTTCTTTGCGC+TGG - chr5.2:21640960-21640979 MS.gene027818:CDS 65.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 21640162 21641775 21640162 ID=MS.gene027818
chr5.2 mRNA 21640162 21641775 21640162 ID=MS.gene027818.t1;Parent=MS.gene027818
chr5.2 exon 21641675 21641775 21641675 ID=MS.gene027818.t1.exon1;Parent=MS.gene027818.t1
chr5.2 CDS 21641675 21641775 21641675 ID=cds.MS.gene027818.t1;Parent=MS.gene027818.t1
chr5.2 exon 21641406 21641519 21641406 ID=MS.gene027818.t1.exon2;Parent=MS.gene027818.t1
chr5.2 CDS 21641406 21641519 21641406 ID=cds.MS.gene027818.t1;Parent=MS.gene027818.t1
chr5.2 exon 21640732 21641245 21640732 ID=MS.gene027818.t1.exon3;Parent=MS.gene027818.t1
chr5.2 CDS 21640732 21641245 21640732 ID=cds.MS.gene027818.t1;Parent=MS.gene027818.t1
chr5.2 exon 21640486 21640639 21640486 ID=MS.gene027818.t1.exon4;Parent=MS.gene027818.t1
chr5.2 CDS 21640486 21640639 21640486 ID=cds.MS.gene027818.t1;Parent=MS.gene027818.t1
chr5.2 exon 21640162 21640367 21640162 ID=MS.gene027818.t1.exon5;Parent=MS.gene027818.t1
chr5.2 CDS 21640162 21640367 21640162 ID=cds.MS.gene027818.t1;Parent=MS.gene027818.t1
Gene Sequence

>MS.gene027818

ATGGCAACAGAAACTAAAGTGTTAGAGCATCCTAAGAAGGCCTTTGGTTGGGCAGCTAGAGATCCTTCTGGTGTTCTCTCCCCTTTCAATTTCTCCAGAAGGTTTACTTCATTATAATCAACACATCTTTATTTAATGGGATGTTTTAATTTAATTAATATTTGATGTTTAGATATATGATATATGTTTTTTTACTTAATTTAGATCTATGTTGTTAGCTACTAATTAAGCCAACTTTGGTGCATCAAAACTTTAGGGAAACTGGTGAGAAAGATGTAGCATTCAAGGTGTTGTATTGTGGAATATGTCACACTGATCTCCATATGATGAAGAATGAATGGGGCAATTCCATCTATCCATTAGTTCCAGGGTATGCTTCCAACTCCTTCTACTACAATTTTACTTTATTTCTACGTTACTTGGACCATATATATCTTTATACTTACTTTAATGATTAGAGAGTGAAAAGTTGAATGAGTCAATAAAAAGTAAGAGTATATTTGAAATTTGCTTATAATTAATAAATGCAGGCATGAACTTGCGGGTATAGTAACCGAGGTAGGAAGCAAAGTGGAAAAGTTTAAAGTTGGAGACAAAGTAGGTGTGGGATACATGGTTGATTCATGTCGTTCATGCGAAAATTGTGCTGACGATCTTGAGAACTACTGCCCCCAACAAACAGTCACATGTGGTGCCAAGTATCGTGATGGCACCGTCACATATGGAGGTTATTCTGACTCAATGGTTGCAGACGAGCACTTTGTGATTCGCATTCCTGATAACTTACCTCTTGATGTTGCCGGTCCTCTTCTTTGCGCTGGAGTCACAGTGTATAGTCCTCTTCGACATTTTGGACTCGACAAACCTGGTATGAATATAGGTGTTGTTGGTCTTGGTGGACTTGGTCATATGGCTGTGAAATTCGCCAAAGCTTTTGGTGCTAATGTGACCGTAATTAGCACATCTCCTAACAAAGAAAAGGAAGCAATAGAACACTTGGGAGCTGACTCGTTTCTTGTAAGCCGCGATCCAGATCAGATGCAGGTATTTGATTCATTTGATTAATTTGTGAATTTCATTATTTCAGAAATCGAGAATGTTAATCAAGAAACTAATAATGTACCACATTTTAATAGGCTGCAACGGGTACTTTGAATGGTATTATTGACACGGTTTCAGCGAGTCATCCTATTTTACCATTGATTGGTTTATTGAAATCTAATGGAAAGCTTGTAATGGTTGGTGGAGTGGCAAAGCCTCTAGAGCTTCCTGTATTTTCTTTACTTGGAGGTAAGCATATAATTTTTTTTTTCCATAGTATAGTTTTAAGACTAAATGCTAAATATGCAGTTTTAAATTCAAATTATGCAGAATGTTGATACTTGTATAAATTTCACATTATTTGTAGGGAGAAAGTTAGTAGCTGGAAGTTTAATTGGAGGGATAAAGGAGACTCAAGAAATGATTGATTTTGCGGCAAAACACAATGTAAAGCCTGATATTGAGGTTATTCCCATTGATTATGTCAACACAGCAGTGGAGCGTCTCGAGAAAGCAGACGTGAAATATCGATTCGTGATTGATATTGGAAATACGCTGAAACCAAGCCCTTAA
Protein sequence

>MS.gene027818.t1

MATETKVLEHPKKAFGWAARDPSGVLSPFNFSRRETGEKDVAFKVLYCGICHTDLHMMKNEWGNSIYPLVPGHELAGIVTEVGSKVEKFKVGDKVGVGYMVDSCRSCENCADDLENYCPQQTVTCGAKYRDGTVTYGGYSDSMVADEHFVIRIPDNLPLDVAGPLLCAGVTVYSPLRHFGLDKPGMNIGVVGLGGLGHMAVKFAKAFGANVTVISTSPNKEKEAIEHLGADSFLVSRDPDQMQAATGTLNGIIDTVSASHPILPLIGLLKSNGKLVMVGGVAKPLELPVFSLLGGRKLVAGSLIGGIKETQEMIDFAAKHNVKPDIEVIPIDYVNTAVERLEKADVKYRFVIDIGNTLKPSP