AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene027819

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027819.t1 MTR_5g031300 96.961 362 11 0 1 362 1 362 0.0 726
MS.gene027819.t1 MTR_5g031310 83.102 361 61 0 1 361 1 361 0.0 622
MS.gene027819.t1 MTR_5g031305 82.271 361 64 0 1 361 1 361 0.0 621
MS.gene027819.t1 MTR_5g031230 80.110 362 71 1 1 362 1 361 0.0 597
MS.gene027819.t1 MTR_4g115300 79.096 354 74 0 9 362 8 361 0.0 588
MS.gene027819.t1 MTR_5g031360 79.272 357 74 0 5 361 6 362 0.0 581
MS.gene027819.t1 MTR_5g031250 78.177 362 78 1 1 362 1 361 0.0 573
MS.gene027819.t1 MTR_5g031390 79.096 354 74 0 9 362 8 361 0.0 573
MS.gene027819.t1 MTR_5g031210 77.966 354 78 0 9 362 8 361 0.0 557
MS.gene027819.t1 MTR_5g031190 72.881 354 96 0 9 362 8 361 0.0 541
MS.gene027819.t1 MTR_5g031180 77.183 355 81 0 8 362 7 361 0.0 539
MS.gene027819.t1 MTR_8g068190 75.424 354 87 0 8 361 7 360 0.0 532
MS.gene027819.t1 MTR_8g092630 58.402 363 145 2 2 359 53 414 2.76e-148 425
MS.gene027819.t1 MTR_5g031430 60.580 345 136 0 15 359 14 358 7.31e-144 412
MS.gene027819.t1 MTR_5g031250 80.992 242 45 1 1 242 1 241 6.41e-137 390
MS.gene027819.t1 MTR_1g025950 55.874 349 153 1 9 356 7 355 2.04e-129 375
MS.gene027819.t1 MTR_1g107425 53.644 343 159 0 12 354 9 351 1.79e-121 355
MS.gene027819.t1 MTR_2g009810 52.890 346 161 2 15 359 62 406 3.57e-115 340
MS.gene027819.t1 MTR_1g107425 55.072 276 124 0 12 287 9 284 2.55e-96 288
MS.gene027819.t1 MTR_2g009820 51.073 233 109 2 131 359 23 254 4.56e-61 197
MS.gene027819.t1 MTR_5g031290 60.432 139 51 1 227 361 55 193 2.19e-51 170
MS.gene027819.t1 MTR_5g031290 83.951 81 11 2 1 80 1 80 1.21e-40 142
MS.gene027819.t1 MTR_8g095220 26.335 281 177 7 26 280 68 344 1.43e-19 89.7
MS.gene027819.t1 MTR_8g095220 28.452 239 146 6 26 243 68 302 2.53e-19 87.8
MS.gene027819.t1 MTR_0001s0660 27.303 304 193 9 39 319 35 333 6.06e-18 85.1
MS.gene027819.t1 MTR_6g465010 25.263 285 190 7 16 288 3 276 8.22e-17 80.9
MS.gene027819.t1 MTR_6g466320 25.263 285 190 7 16 288 19 292 9.82e-17 80.9
MS.gene027819.t1 MTR_6g465010 25.263 285 190 7 16 288 19 292 9.82e-17 80.9
MS.gene027819.t1 MTR_7g068320 26.301 346 205 12 40 344 48 384 1.25e-13 72.0
MS.gene027819.t1 MTR_3g099460 27.937 315 185 14 40 320 44 350 3.06e-13 70.9
MS.gene027819.t1 MTR_3g099460 27.937 315 185 14 40 320 37 343 3.60e-13 70.5
MS.gene027819.t1 MTR_3g089977 21.203 349 236 11 40 355 37 379 1.33e-12 68.6
MS.gene027819.t1 MTR_3g089940 21.958 378 254 12 1 345 1 370 1.69e-12 68.6
MS.gene027819.t1 MTR_7g068330 27.616 344 202 17 40 344 41 376 1.71e-12 68.6
MS.gene027819.t1 MTR_3g089940 21.958 378 254 12 1 345 1 370 1.84e-12 68.2
MS.gene027819.t1 MTR_5g045710 24.024 333 222 8 39 343 35 364 1.86e-12 68.2
MS.gene027819.t1 MTR_7g099040 26.019 319 197 13 40 328 38 347 1.90e-12 68.2
MS.gene027819.t1 MTR_3g079180 26.846 298 186 13 39 308 40 333 3.48e-11 64.3
MS.gene027819.t1 MTR_5g031380 58.537 41 17 0 81 121 19 59 3.63e-11 60.1
MS.gene027819.t1 MTR_3g089940 23.345 287 189 8 1 263 1 280 3.93e-11 63.9
MS.gene027819.t1 MTR_1g069430 25.364 343 214 15 1 313 1 331 4.00e-11 64.3
MS.gene027819.t1 MTR_3g089970 21.534 339 228 10 40 345 38 371 4.37e-11 63.9
MS.gene027819.t1 MTR_3g099460 32.886 149 74 5 40 167 37 180 5.46e-11 63.5
MS.gene027819.t1 MTR_3g099460 32.886 149 74 5 40 167 44 187 5.64e-11 63.5
MS.gene027819.t1 MTR_3g089940 23.345 287 189 8 1 263 1 280 5.66e-11 63.5
MS.gene027819.t1 MTR_5g087710 25.513 341 214 11 40 345 46 381 6.61e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027819.t1 AT4G37980 69.429 350 107 0 12 361 7 356 1.90e-173 487
MS.gene027819.t1 AT4G37990 69.143 350 108 0 12 361 7 356 2.04e-167 471
MS.gene027819.t1 AT4G37970 61.190 353 137 0 9 361 9 361 2.61e-162 459
MS.gene027819.t1 AT4G39330 60.057 353 141 0 8 360 7 359 8.10e-161 455
MS.gene027819.t1 AT2G21890 58.120 351 146 1 13 362 6 356 1.20e-150 429
MS.gene027819.t1 AT2G21730 58.523 352 144 2 13 362 6 357 6.86e-150 427
MS.gene027819.t1 AT4G37980 69.097 288 89 0 12 299 7 294 8.15e-135 386
MS.gene027819.t1 AT4G39330 59.322 295 120 0 8 302 7 301 5.41e-128 370
MS.gene027819.t1 AT4G37970 59.310 290 118 0 9 298 9 298 1.23e-123 358
MS.gene027819.t1 AT3G19450 53.757 346 160 0 12 357 9 354 1.66e-115 340
MS.gene027819.t1 AT1G72680 49.271 343 173 1 17 359 14 355 5.19e-111 328
MS.gene027819.t1 AT4G34230 52.299 348 166 0 7 354 3 350 2.58e-105 313
MS.gene027819.t1 AT4G34230 52.011 348 167 0 7 354 3 350 1.64e-104 311
MS.gene027819.t1 AT4G37970 59.494 237 96 0 9 245 9 245 2.42e-99 295
MS.gene027819.t1 AT4G37970 60.000 235 94 0 9 243 9 243 4.09e-99 294
MS.gene027819.t1 AT5G63620 25.795 283 178 8 25 280 69 346 9.01e-20 90.5
MS.gene027819.t1 AT5G63620 25.887 282 177 8 26 280 70 346 9.36e-20 90.5
MS.gene027819.t1 AT5G63620 28.634 227 137 6 25 230 69 291 2.91e-19 87.8
MS.gene027819.t1 AT1G22430 26.183 317 202 12 40 328 42 354 1.96e-17 83.2
MS.gene027819.t1 AT1G22430 26.183 317 202 12 40 328 42 354 1.96e-17 83.2
MS.gene027819.t1 AT5G51970 25.632 277 183 7 16 280 21 286 6.52e-17 81.6
MS.gene027819.t1 AT5G51970 25.632 277 183 7 16 280 21 286 6.52e-17 81.6
MS.gene027819.t1 AT1G64710 26.608 342 208 13 40 344 13 348 1.26e-16 80.5
MS.gene027819.t1 AT1G64710 26.608 342 208 13 40 344 36 371 1.46e-16 80.5
MS.gene027819.t1 AT5G43940 25.360 347 220 12 40 353 36 376 5.00e-16 79.0
MS.gene027819.t1 AT5G43940 25.360 347 220 12 40 353 48 388 6.05e-16 79.0
MS.gene027819.t1 AT1G32780 25.886 367 215 16 40 359 37 393 1.13e-15 78.2
MS.gene027819.t1 AT4G22110 24.927 341 215 16 40 344 43 378 2.80e-15 77.0
MS.gene027819.t1 AT4G22110 24.927 341 215 16 40 344 43 378 2.80e-15 77.0
MS.gene027819.t1 AT1G22430 26.500 200 128 5 40 222 42 239 3.61e-13 70.1
MS.gene027819.t1 AT1G22430 26.500 200 128 5 40 222 42 239 4.32e-13 69.3
MS.gene027819.t1 AT1G22430 26.500 200 128 5 40 222 42 239 4.32e-13 69.3
MS.gene027819.t1 AT1G22440 25.217 345 211 15 40 345 40 376 8.59e-13 69.3
MS.gene027819.t1 AT5G42250 25.000 296 193 9 40 308 45 338 3.68e-12 67.4
MS.gene027819.t1 AT1G77120 22.029 345 238 11 40 355 36 378 2.16e-11 65.1
MS.gene027819.t1 AT4G22110 26.267 217 138 9 40 237 43 256 2.34e-11 64.3

Find 88 sgRNAs with CRISPR-Local

Find 149 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCACATGTGACTACAAATT+TGG 0.137677 5.2:+21624413 None:intergenic
TATCGATTTGTGATTGATAT+TGG 0.198958 5.2:-21623434 MS.gene027819:CDS
TCCATTTATCCACTTGTTCC+TGG 0.254331 5.2:-21624810 MS.gene027819:CDS
CCCAGGAACAAGTGGATAAA+TGG 0.269203 5.2:+21624809 None:intergenic
CAAGATTGTCATTACAATCT+TGG 0.292744 5.2:+21624448 None:intergenic
CCCCTTTCAATTTCTCTAGA+AGG 0.302235 5.2:-21625531 MS.gene027819:intron
CCACATGTGACTACAAATTT+GGG 0.308824 5.2:+21624414 None:intergenic
CCTGAATATACTTTAATTCA+AGG 0.316011 5.2:-21623811 MS.gene027819:intron
GTGAAATTTGCCAAAGCTTT+CGG 0.316975 5.2:-21624168 MS.gene027819:CDS
AAGTCAAGTTGGAGGGTTAA+AGG 0.329887 5.2:-21623573 MS.gene027819:CDS
GCTGCAATTGGTACTTTAGA+TGG 0.335712 5.2:-21623943 MS.gene027819:CDS
TCTTGGTGGACTTGGCCATA+TGG 0.337845 5.2:-21624193 MS.gene027819:CDS
GGTTTACATATAGGTGTTGT+TGG 0.340460 5.2:-21624216 MS.gene027819:CDS
CCTTGAATTAAAGTATATTC+AGG 0.347223 5.2:+21623811 None:intergenic
GCCCATCCAAAGGCCTTCTT+AGG 0.354227 5.2:+21625580 None:intergenic
CATATAGGTGTTGTTGGTCT+TGG 0.355070 5.2:-21624210 MS.gene027819:CDS
CCCATTCATTCTTCGCCATA+TGG 0.361451 5.2:+21624838 None:intergenic
TAGTGACCCAAAGCAGATTC+AGG 0.370893 5.2:-21624061 MS.gene027819:intron
TATGGTACCTGAATCTGCTT+TGG 0.371107 5.2:+21624054 None:intergenic
GTTGTTGGTCTTGGTGGACT+TGG 0.372585 5.2:-21624201 MS.gene027819:CDS
AACATACCTCTTGAATTTGC+TGG 0.378852 5.2:-21624303 MS.gene027819:CDS
CATCCTCTCTTACCAATGAT+TGG 0.384438 5.2:-21623895 MS.gene027819:CDS
TCCTGCAAAGTTTAAGAGCT+TGG 0.390576 5.2:+21623398 None:intergenic
GTTAGGCGATGTACTAATTA+CGG 0.396532 5.2:+21624134 None:intergenic
CACATTAGCACCGAAAGCTT+TGG 0.413943 5.2:+21624158 None:intergenic
AAATATAGGCATGAGCTTGC+GGG 0.415703 5.2:-21624561 MS.gene027819:intron
GATAAGCCTGGTTTACATAT+AGG 0.416506 5.2:-21624225 MS.gene027819:CDS
GCTGGTCCTCTCCTTTGTGC+TGG 0.419633 5.2:-21624285 MS.gene027819:CDS
AAATCGAAGAGGACTATATA+CGG 0.426441 5.2:+21624257 None:intergenic
CCATTTATCCACTTGTTCCT+GGG 0.426610 5.2:-21624809 MS.gene027819:intron
ATAGGTGTTGTTGGTCTTGG+TGG 0.429450 5.2:-21624207 MS.gene027819:CDS
GAGCATCCTAAGAAGGCCTT+TGG 0.436962 5.2:-21625586 MS.gene027819:CDS
TCCATATGGCGAAGAATGAA+TGG 0.444643 5.2:-21624839 MS.gene027819:CDS
TGGCAAATTTCACAGCCATA+TGG 0.446571 5.2:+21624178 None:intergenic
AATTCAAGAGGTATGTTGTC+AGG 0.452593 5.2:+21624309 None:intergenic
AAAGTGGAGAAATTTAAAGT+TGG 0.458834 5.2:-21624516 MS.gene027819:CDS
CGTGATGGAACCATCACATA+TGG 0.470934 5.2:-21624381 MS.gene027819:CDS
ATGGTACCTGAATCTGCTTT+GGG 0.471446 5.2:+21624055 None:intergenic
GATGGTTCCATCACGATACT+TGG 0.471578 5.2:+21624389 None:intergenic
TTTAAAGTTGGAGACAAAGT+AGG 0.474364 5.2:-21624504 MS.gene027819:CDS
ATCGGAACAACCTCAATATC+CGG 0.476248 5.2:+21623506 None:intergenic
ACCTTCTAGAGAAATTGAAA+GGG 0.479707 5.2:+21625530 None:intergenic
TGGGCAGCTAGAGATCATTC+TGG 0.480669 5.2:-21625562 MS.gene027819:CDS
CAATGTAAAGCCGGATATTG+AGG 0.481193 5.2:-21623516 MS.gene027819:CDS
ATTGGTCTCTTGAAGTCTCA+TGG 0.481856 5.2:-21623877 MS.gene027819:CDS
AACCTTCTAGAGAAATTGAA+AGG 0.492744 5.2:+21625529 None:intergenic
CCATATGGCGAAGAATGAAT+GGG 0.494659 5.2:-21624838 MS.gene027819:CDS
AGCAATGGAGCGTCTTGCTA+AGG 0.496567 5.2:-21623468 MS.gene027819:CDS
CATGGAAAGCTTATAATGCT+TGG 0.504127 5.2:-21623859 MS.gene027819:CDS
ATGTCACTCTGATCTCCATA+TGG 0.507753 5.2:-21624853 MS.gene027819:CDS
ATTAGTAGAGCATCCTAAGA+AGG 0.516837 5.2:-21625593 MS.gene027819:CDS
ATATTAGCTGGAAGTCAAGT+TGG 0.518997 5.2:-21623584 MS.gene027819:CDS
TCCTAAGAAGGCCTTTGGAT+GGG 0.520806 5.2:-21625581 MS.gene027819:CDS
GAGAGGATGAACAGCTGAAA+CGG 0.539263 5.2:+21623909 None:intergenic
TACATCGCCTAACAAAGAAA+AGG 0.540053 5.2:-21624124 MS.gene027819:CDS
TAAATATAGGCATGAGCTTG+CGG 0.545464 5.2:-21624562 MS.gene027819:intron
GTATATTCAGGAAGCTGCAA+AGG 0.546317 5.2:+21623823 None:intergenic
CTCCACTTTACTTCCCACTT+CGG 0.546580 5.2:+21624530 None:intergenic
TGGAGGTTACTCCGACTCAA+TGG 0.547063 5.2:-21624361 MS.gene027819:CDS
ATCCTAAGAAGGCCTTTGGA+TGG 0.552765 5.2:-21625582 MS.gene027819:CDS
AACCGAAGTGGGAAGTAAAG+TGG 0.553032 5.2:-21624532 MS.gene027819:CDS
GACTTCAAGAGACCAATCAT+TGG 0.565239 5.2:+21623883 None:intergenic
GCTGTGTTGACATAATCAAT+CGG 0.567158 5.2:+21623488 None:intergenic
GCAATGGAGCGTCTTGCTAA+GGG 0.567348 5.2:-21623467 MS.gene027819:CDS
GTCAGAACACAATGTAAAGC+CGG 0.569274 5.2:-21623525 MS.gene027819:CDS
ATCTCTAGCTGCCCATCCAA+AGG 0.572515 5.2:+21625570 None:intergenic
AAAACATACCCAGGAACAAG+TGG 0.582294 5.2:+21624801 None:intergenic
CTCGTCTGCAACCATTGAGT+CGG 0.587984 5.2:+21624350 None:intergenic
AGTTGGAGACAAAGTAGGTG+TGG 0.589771 5.2:-21624499 MS.gene027819:CDS
AGACCAATCATTGGTAAGAG+AGG 0.590479 5.2:+21623892 None:intergenic
AAGGAAGCAATAGAACACTT+AGG 0.590978 5.2:-21624105 MS.gene027819:CDS
TTAGCTGGAAGTCAAGTTGG+AGG 0.591625 5.2:-21623581 MS.gene027819:CDS
TAGCTGGAAGTCAAGTTGGA+GGG 0.608185 5.2:-21623580 MS.gene027819:CDS
TGATTATGTCAACACAGCAA+TGG 0.611967 5.2:-21623483 MS.gene027819:CDS
GTTGGAGACAAAGTAGGTGT+GGG 0.615995 5.2:-21624498 MS.gene027819:CDS
GGAGTAACCTCCATATGTGA+TGG 0.622572 5.2:+21624371 None:intergenic
GCGGGTGTAGTAACCGAAGT+GGG 0.625376 5.2:-21624543 MS.gene027819:CDS
TGCGGGTGTAGTAACCGAAG+TGG 0.637661 5.2:-21624544 MS.gene027819:CDS
ACAACACCTATATGTAAACC+AGG 0.637734 5.2:+21624219 None:intergenic
CCCAAATTTGTAGTCACATG+TGG 0.638631 5.2:-21624414 MS.gene027819:CDS
AGAGGACCAGCAAATTCAAG+AGG 0.642176 5.2:+21624297 None:intergenic
CCTTCTAGAGAAATTGAAAG+GGG 0.647504 5.2:+21625531 None:intergenic
TGTGGTGCCAAGTATCGTGA+TGG 0.659003 5.2:-21624396 MS.gene027819:CDS
GATGGAACCATCACATATGG+AGG 0.662810 5.2:-21624378 MS.gene027819:CDS
CACATGTGACTACAAATTTG+GGG 0.672670 5.2:+21624415 None:intergenic
GTGACACCAGCACAAAGGAG+AGG 0.674659 5.2:+21624279 None:intergenic
CATATGGCGAAGAATGAATG+GGG 0.702857 5.2:-21624837 MS.gene027819:CDS
ATACGGTGACACCAGCACAA+AGG 0.718821 5.2:+21624274 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATATATATATATATATA+TGG + chr5.2:21625276-21625257 None:intergenic 0.0%
!! TAATAACAAAGATAATAAAA+AGG + chr5.2:21624249-21624268 None:intergenic 10.0%
!! ACATAGATCTAAATAATTTA+AGG + chr5.2:21624054-21624073 None:intergenic 15.0%
!! CAAAATAAATAAGAATAGAT+AGG - chr5.2:21623720-21623739 MS.gene027819:intron 15.0%
!! CATAGATCTAAATAATTTAA+GGG + chr5.2:21624053-21624072 None:intergenic 15.0%
!! GTAATTGATGTAATAAATAT+AGG - chr5.2:21624444-21624463 MS.gene027819:CDS 15.0%
!! TTAATTTCAAATTACTGATA+GGG - chr5.2:21625406-21625425 MS.gene027819:intron 15.0%
!!! CTATCTATTCTTATTTATTT+TGG + chr5.2:21623722-21623741 None:intergenic 15.0%
!!! GTTTAATTTAGATGTAATTT+TGG + chr5.2:21623932-21623951 None:intergenic 15.0%
!! AAAAGATTAAAACAAGGAAT+TGG - chr5.2:21623633-21623652 MS.gene027819:intron 20.0%
!! ACTGATAAAAGATTAAAACA+AGG - chr5.2:21623627-21623646 MS.gene027819:intron 20.0%
!! ATATATATATTCCCTTAGAA+TGG - chr5.2:21625306-21625325 MS.gene027819:intron 20.0%
!! ATTATTTAGATCTATGTTTC+TGG - chr5.2:21624057-21624076 MS.gene027819:intron 20.0%
!! CAAATTAATCATATACTGTA+TGG + chr5.2:21624986-21625005 None:intergenic 20.0%
!! GATACTAATTAATTCAACTT+TGG - chr5.2:21624079-21624098 MS.gene027819:CDS 20.0%
!! GTTAATTTCAAATTACTGAT+AGG - chr5.2:21625405-21625424 MS.gene027819:intron 20.0%
!! TAAATAAGAATAGATAGGTT+CGG - chr5.2:21623725-21623744 MS.gene027819:intron 20.0%
!! TAATATTTCTTGAATGTGTT+TGG - chr5.2:21623695-21623714 MS.gene027819:intron 20.0%
!! TATGCATTAAATTAATGAAG+AGG + chr5.2:21623530-21623549 None:intergenic 20.0%
!!! AACTAACAAATTTTAAGTAG+AGG + chr5.2:21623847-21623866 None:intergenic 20.0%
!!! ATCACTAATTTTTAATGATG+TGG + chr5.2:21623771-21623790 None:intergenic 20.0%
!!! TTGTTTTAATCTTTTATCAG+TGG + chr5.2:21623628-21623647 None:intergenic 20.0%
! ATAATTGAACCAAACTTCAT+TGG - chr5.2:21624022-21624041 MS.gene027819:intron 25.0%
! ATTAATTTCCTTGAAACTAG+TGG + chr5.2:21623586-21623605 None:intergenic 25.0%
! CCTGAATATACTTTAATTCA+AGG - chr5.2:21625208-21625227 MS.gene027819:intron 25.0%
! CCTTGAATTAAAGTATATTC+AGG + chr5.2:21625211-21625230 None:intergenic 25.0%
! GAGTATTATATTTACAGACA+TGG + chr5.2:21624285-21624304 None:intergenic 25.0%
! TTTATCAGTGGAAAAAGAAA+AGG + chr5.2:21623616-21623635 None:intergenic 25.0%
!! AAAGTGGAGAAATTTAAAGT+TGG - chr5.2:21624503-21624522 MS.gene027819:CDS 25.0%
!! AAGAATGTATGATGATAATG+CGG + chr5.2:21623955-21623974 None:intergenic 25.0%
!! GGCAAAAAATAATTCTAGTT+TGG + chr5.2:21624000-21624019 None:intergenic 25.0%
!!! AATTCTAGTTTGGTTCATTT+TGG + chr5.2:21623990-21624009 None:intergenic 25.0%
!!! ATAATATTTTAGGCTGCAAT+TGG - chr5.2:21625064-21625083 MS.gene027819:intron 25.0%
!!! TATCGATTTGTGATTGATAT+TGG - chr5.2:21625585-21625604 MS.gene027819:CDS 25.0%
AAATCGAAGAGGACTATATA+CGG + chr5.2:21624765-21624784 None:intergenic 30.0%
ATAGGGAGAAAAATATTAGC+TGG - chr5.2:21625423-21625442 MS.gene027819:intron 30.0%
CAAGATTGTCATTACAATCT+TGG + chr5.2:21624574-21624593 None:intergenic 30.0%
GATTAAAACAAGGAATTGGA+TGG - chr5.2:21623637-21623656 MS.gene027819:intron 30.0%
TGAAAAAGATGTAGCATTCA+AGG - chr5.2:21624127-21624146 MS.gene027819:CDS 30.0%
! AACCTTCTAGAGAAATTGAA+AGG + chr5.2:21623493-21623512 None:intergenic 30.0%
! AACTTTTTGACACACATCAA+AGG - chr5.2:21623802-21623821 MS.gene027819:intron 30.0%
! ACCTTCTAGAGAAATTGAAA+GGG + chr5.2:21623492-21623511 None:intergenic 30.0%
! AGAATGTATGATGATAATGC+GGG + chr5.2:21623954-21623973 None:intergenic 30.0%
! AGCAAAAGTATATGCTACAT+TGG + chr5.2:21624424-21624443 None:intergenic 30.0%
! ATTGCTTCCTTTTCTTTGTT+AGG + chr5.2:21624905-21624924 None:intergenic 30.0%
! TGAAGTTTGGTTCAATTATG+AGG + chr5.2:21624021-21624040 None:intergenic 30.0%
! TTTAAAGTTGGAGACAAAGT+AGG - chr5.2:21624515-21624534 MS.gene027819:CDS 30.0%
!! CATTCAAGGTTTTGTATTGT+GGG - chr5.2:21624141-21624160 MS.gene027819:CDS 30.0%
!! TTTGGTGCACCAAAATTTTA+GGG - chr5.2:21624097-21624116 MS.gene027819:CDS 30.0%
!!! TATAGTCCTCTTCGATTTTT+TGG - chr5.2:21624767-21624786 MS.gene027819:intron 30.0%
AACATACCTCTTGAATTTGC+TGG - chr5.2:21624716-21624735 MS.gene027819:intron 35.0%
AAGGAAGCAATAGAACACTT+AGG - chr5.2:21624914-21624933 MS.gene027819:intron 35.0%
AATTTCCTTGAAACTAGTGG+AGG + chr5.2:21623583-21623602 None:intergenic 35.0%
ACAACACCTATATGTAAACC+AGG + chr5.2:21624803-21624822 None:intergenic 35.0%
ACCACATGTGACTACAAATT+TGG + chr5.2:21624609-21624628 None:intergenic 35.0%
ATATTAGCTGGAAGTCAAGT+TGG - chr5.2:21625435-21625454 MS.gene027819:intron 35.0%
ATATTCCCTTAGAATGGTTC+AGG - chr5.2:21625312-21625331 MS.gene027819:intron 35.0%
ATTTCCTTGAAACTAGTGGA+GGG + chr5.2:21623582-21623601 None:intergenic 35.0%
CACATGTGACTACAAATTTG+GGG + chr5.2:21624607-21624626 None:intergenic 35.0%
CATGGAAAGCTTATAATGCT+TGG - chr5.2:21625160-21625179 MS.gene027819:intron 35.0%
CCACATGTGACTACAAATTT+GGG + chr5.2:21624608-21624627 None:intergenic 35.0%
GATAAGCCTGGTTTACATAT+AGG - chr5.2:21624794-21624813 MS.gene027819:intron 35.0%
GTGAAATTTGCCAAAGCTTT+CGG - chr5.2:21624851-21624870 MS.gene027819:CDS 35.0%
GTGTGTCAAAAAGTTAGTAG+CGG + chr5.2:21623797-21623816 None:intergenic 35.0%
GTTAGGCGATGTACTAATTA+CGG + chr5.2:21624888-21624907 None:intergenic 35.0%
TAAATATAGGCATGAGCTTG+CGG - chr5.2:21624457-21624476 MS.gene027819:CDS 35.0%
TACATCGCCTAACAAAGAAA+AGG - chr5.2:21624895-21624914 MS.gene027819:CDS 35.0%
TGATTATGTCAACACAGCAA+TGG - chr5.2:21625536-21625555 MS.gene027819:CDS 35.0%
TTTCTCCTGAACCATTCTAA+GGG + chr5.2:21625320-21625339 None:intergenic 35.0%
! AATTCAAGAGGTATGTTGTC+AGG + chr5.2:21624713-21624732 None:intergenic 35.0%
! ATTAGTAGAGCATCCTAAGA+AGG - chr5.2:21623426-21623445 MS.gene027819:CDS 35.0%
! CACCTGTTTCCCTAAAATTT+TGG + chr5.2:21624109-21624128 None:intergenic 35.0%
! CCTTCTAGAGAAATTGAAAG+GGG + chr5.2:21623491-21623510 None:intergenic 35.0%
! GCTGTGTTGACATAATCAAT+CGG + chr5.2:21625534-21625553 None:intergenic 35.0%
! GGTTTACATATAGGTGTTGT+TGG - chr5.2:21624803-21624822 MS.gene027819:intron 35.0%
! TTTTCTCCTGAACCATTCTA+AGG + chr5.2:21625321-21625340 None:intergenic 35.0%
!! CACCAAAATTTTAGGGAAAC+AGG - chr5.2:21624104-21624123 MS.gene027819:CDS 35.0%
!! CTTTGGTGCACCAAAATTTT+AGG - chr5.2:21624096-21624115 MS.gene027819:CDS 35.0%
!! GCATTCAAGGTTTTGTATTG+TGG - chr5.2:21624140-21624159 MS.gene027819:CDS 35.0%
AAAACATACCCAGGAACAAG+TGG + chr5.2:21624221-21624240 None:intergenic 40.0%
AAATATAGGCATGAGCTTGC+GGG - chr5.2:21624458-21624477 MS.gene027819:CDS 40.0%
AAGGAGTTCAAAACATACCC+AGG + chr5.2:21624230-21624249 None:intergenic 40.0%
AGACCAATCATTGGTAAGAG+AGG + chr5.2:21625130-21625149 None:intergenic 40.0%
ATCGGAACAACCTCAATATC+CGG + chr5.2:21625516-21625535 None:intergenic 40.0%
ATGGTACCTGAATCTGCTTT+GGG + chr5.2:21624967-21624986 None:intergenic 40.0%
ATGTCACTCTGATCTCCATA+TGG - chr5.2:21624166-21624185 MS.gene027819:CDS 40.0%
CAATGTAAAGCCGGATATTG+AGG - chr5.2:21625503-21625522 MS.gene027819:intron 40.0%
CATATGGCGAAGAATGAATG+GGG - chr5.2:21624182-21624201 MS.gene027819:CDS 40.0%
CATCCTCTCTTACCAATGAT+TGG - chr5.2:21625124-21625143 MS.gene027819:intron 40.0%
CCATATGGCGAAGAATGAAT+GGG - chr5.2:21624181-21624200 MS.gene027819:CDS 40.0%
CCATTTATCCACTTGTTCCT+GGG - chr5.2:21624210-21624229 MS.gene027819:CDS 40.0%
CCCAAATTTGTAGTCACATG+TGG - chr5.2:21624605-21624624 MS.gene027819:intron 40.0%
CCCCTTTCAATTTCTCTAGA+AGG - chr5.2:21623488-21623507 MS.gene027819:CDS 40.0%
GACTTCAAGAGACCAATCAT+TGG + chr5.2:21625139-21625158 None:intergenic 40.0%
GCTGCAATTGGTACTTTAGA+TGG - chr5.2:21625076-21625095 MS.gene027819:intron 40.0%
GTATATTCAGGAAGCTGCAA+AGG + chr5.2:21625199-21625218 None:intergenic 40.0%
GTCAGAACACAATGTAAAGC+CGG - chr5.2:21625494-21625513 MS.gene027819:intron 40.0%
TATGGTACCTGAATCTGCTT+TGG + chr5.2:21624968-21624987 None:intergenic 40.0%
TCCATATGGCGAAGAATGAA+TGG - chr5.2:21624180-21624199 MS.gene027819:CDS 40.0%
TCCATTTATCCACTTGTTCC+TGG - chr5.2:21624209-21624228 MS.gene027819:CDS 40.0%
TCGAGTCCAAAAAATCGAAG+AGG + chr5.2:21624776-21624795 None:intergenic 40.0%
TGGCAAATTTCACAGCCATA+TGG + chr5.2:21624844-21624863 None:intergenic 40.0%
TTTCCTTGAAACTAGTGGAG+GGG + chr5.2:21623581-21623600 None:intergenic 40.0%
! ATTGGTCTCTTGAAGTCTCA+TGG - chr5.2:21625142-21625161 MS.gene027819:intron 40.0%
!! AAGTAGAGGCATCAAGATGT+TGG + chr5.2:21623833-21623852 None:intergenic 40.0%
!! AAGTCAAGTTGGAGGGTTAA+AGG - chr5.2:21625446-21625465 MS.gene027819:intron 40.0%
!! CATATAGGTGTTGTTGGTCT+TGG - chr5.2:21624809-21624828 MS.gene027819:intron 40.0%
!!! TTTTTTGGACTCGATAAGCC+TGG - chr5.2:21624782-21624801 MS.gene027819:intron 40.0%
AACCGAAGTGGGAAGTAAAG+TGG - chr5.2:21624487-21624506 MS.gene027819:CDS 45.0%
AGAGGACCAGCAAATTCAAG+AGG + chr5.2:21624725-21624744 None:intergenic 45.0%
AGTTGGAGACAAAGTAGGTG+TGG - chr5.2:21624520-21624539 MS.gene027819:CDS 45.0%
ATCCTAAGAAGGCCTTTGGA+TGG - chr5.2:21623437-21623456 MS.gene027819:CDS 45.0%
CCCAGGAACAAGTGGATAAA+TGG + chr5.2:21624213-21624232 None:intergenic 45.0%
CCCATTCATTCTTCGCCATA+TGG + chr5.2:21624184-21624203 None:intergenic 45.0%
CTCCACTTTACTTCCCACTT+CGG + chr5.2:21624492-21624511 None:intergenic 45.0%
GAGAGGATGAACAGCTGAAA+CGG + chr5.2:21625113-21625132 None:intergenic 45.0%
GATGGAACCATCACATATGG+AGG - chr5.2:21624641-21624660 MS.gene027819:intron 45.0%
GATGGTTCCATCACGATACT+TGG + chr5.2:21624633-21624652 None:intergenic 45.0%
GGAGTAACCTCCATATGTGA+TGG + chr5.2:21624651-21624670 None:intergenic 45.0%
GTTGGAGACAAAGTAGGTGT+GGG - chr5.2:21624521-21624540 MS.gene027819:CDS 45.0%
TAGTGACCCAAAGCAGATTC+AGG - chr5.2:21624958-21624977 MS.gene027819:intron 45.0%
TCCTAAGAAGGCCTTTGGAT+GGG - chr5.2:21623438-21623457 MS.gene027819:CDS 45.0%
TTCCTTGAAACTAGTGGAGG+GGG + chr5.2:21623580-21623599 None:intergenic 45.0%
! CACATTAGCACCGAAAGCTT+TGG + chr5.2:21624864-21624883 None:intergenic 45.0%
! CGTGATGGAACCATCACATA+TGG - chr5.2:21624638-21624657 MS.gene027819:intron 45.0%
!! AGGGTGTTGCCAATGAAGTT+TGG + chr5.2:21624034-21624053 None:intergenic 45.0%
!! ATAGGTGTTGTTGGTCTTGG+TGG - chr5.2:21624812-21624831 MS.gene027819:CDS 45.0%
!! TAGCTGGAAGTCAAGTTGGA+GGG - chr5.2:21625439-21625458 MS.gene027819:intron 45.0%
!! TTAGCTGGAAGTCAAGTTGG+AGG - chr5.2:21625438-21625457 MS.gene027819:intron 45.0%
!! TAATTAAAATATAAATGAAA+GGG - chr5.2:21624385-21624404 MS.gene027819:CDS 5.0%
!! TTAATTAAAATATAAATGAA+AGG - chr5.2:21624384-21624403 MS.gene027819:CDS 5.0%
!!! AATATTATTGATAATATTTT+AGG - chr5.2:21625054-21625073 MS.gene027819:intron 5.0%
ATACGGTGACACCAGCACAA+AGG + chr5.2:21624748-21624767 None:intergenic 50.0%
ATCTCTAGCTGCCCATCCAA+AGG + chr5.2:21623452-21623471 None:intergenic 50.0%
CTCGTCTGCAACCATTGAGT+CGG + chr5.2:21624672-21624691 None:intergenic 50.0%
GAGCATCCTAAGAAGGCCTT+TGG - chr5.2:21623433-21623452 MS.gene027819:CDS 50.0%
TGGAGGTTACTCCGACTCAA+TGG - chr5.2:21624658-21624677 MS.gene027819:intron 50.0%
TGGGCAGCTAGAGATCATTC+TGG - chr5.2:21623457-21623476 MS.gene027819:CDS 50.0%
! AGCAATGGAGCGTCTTGCTA+AGG - chr5.2:21625551-21625570 MS.gene027819:CDS 50.0%
! GCAATGGAGCGTCTTGCTAA+GGG - chr5.2:21625552-21625571 MS.gene027819:CDS 50.0%
! TGTGGTGCCAAGTATCGTGA+TGG - chr5.2:21624623-21624642 MS.gene027819:intron 50.0%
! TTCCCCCTCCACTAGTTTCA+AGG - chr5.2:21623575-21623594 MS.gene027819:CDS 50.0%
!! GTTGTTGGTCTTGGTGGACT+TGG - chr5.2:21624818-21624837 MS.gene027819:CDS 50.0%
!! TCTTGGTGGACTTGGCCATA+TGG - chr5.2:21624826-21624845 MS.gene027819:CDS 50.0%
GCCCATCCAAAGGCCTTCTT+AGG + chr5.2:21623442-21623461 None:intergenic 55.0%
GCGGGTGTAGTAACCGAAGT+GGG - chr5.2:21624476-21624495 MS.gene027819:CDS 55.0%
GTGACACCAGCACAAAGGAG+AGG + chr5.2:21624743-21624762 None:intergenic 55.0%
TGCGGGTGTAGTAACCGAAG+TGG - chr5.2:21624475-21624494 MS.gene027819:CDS 55.0%
GCTGGTCCTCTCCTTTGTGC+TGG - chr5.2:21624734-21624753 MS.gene027819:intron 60.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 21623409 21625632 21623409 ID=MS.gene027819
chr5.2 mRNA 21623409 21625632 21623409 ID=MS.gene027819.t1;Parent=MS.gene027819
chr5.2 exon 21625532 21625632 21625532 ID=MS.gene027819.t1.exon1;Parent=MS.gene027819.t1
chr5.2 CDS 21625532 21625632 21625532 ID=cds.MS.gene027819.t1;Parent=MS.gene027819.t1
chr5.2 exon 21624810 21624923 21624810 ID=MS.gene027819.t1.exon2;Parent=MS.gene027819.t1
chr5.2 CDS 21624810 21624923 21624810 ID=cds.MS.gene027819.t1;Parent=MS.gene027819.t1
chr5.2 exon 21624062 21624575 21624062 ID=MS.gene027819.t1.exon3;Parent=MS.gene027819.t1
chr5.2 CDS 21624062 21624575 21624062 ID=cds.MS.gene027819.t1;Parent=MS.gene027819.t1
chr5.2 exon 21623812 21623965 21623812 ID=MS.gene027819.t1.exon4;Parent=MS.gene027819.t1
chr5.2 CDS 21623812 21623965 21623812 ID=cds.MS.gene027819.t1;Parent=MS.gene027819.t1
chr5.2 exon 21623409 21623614 21623409 ID=MS.gene027819.t1.exon5;Parent=MS.gene027819.t1
chr5.2 CDS 21623409 21623614 21623409 ID=cds.MS.gene027819.t1;Parent=MS.gene027819.t1
Gene Sequence

>MS.gene027819

ATGGCAACAGAAACTAAATTAGTAGAGCATCCTAAGAAGGCCTTTGGATGGGCAGCTAGAGATCATTCTGGTGTTCTCTCCCCTTTCAATTTCTCTAGAAGGTTTTACTTCATGATCACCTCTTCATTAATTTAATGCATATTTCTAACATTATTTGTTGCATCATTTCCCCCTCCACTAGTTTCAAGGAAATTAATATTTTTGCCTTTTCTTTTTCCACTGATAAAAGATTAAAACAAGGAATTGGATGGAATGTGCTAATTGAATGAATATATTTGATGTTTACTAATATTTCTTGAATGTGTTTGGACCAAAATAAATAAGAATAGATAGGTTCGGTTTAGTTTTACTAAACTAAACCACATCATTAAAAATTAGTGATATACCGCTACTAACTTTTTGACACACATCAAAGGATCTACCAACATCTTGATGCCTCTACTTAAAATTTGTTAGTTTTGAAAATCTATTAGTTTTAATATAAGTGTTAAAATAATTTTTAAGATTGTAATTTAGATAACCAAAATTACATCTAAATTAAACCCGCATTATCATCATACATTCTTTTAAAGAGACATCCAAAATGAACCAAACTAGAATTATTTTTTGCCTCATAATTGAACCAAACTTCATTGGCAACACCCTTAAATTATTTAGATCTATGTTTCTGGATACTAATTAATTCAACTTTGGTGCACCAAAATTTTAGGGAAACAGGTGAAAAAGATGTAGCATTCAAGGTTTTGTATTGTGGGATATGTCACTCTGATCTCCATATGGCGAAGAATGAATGGGGCACTTCCATTTATCCACTTGTTCCTGGGTATGTTTTGAACTCCTTTTTATTATCTTTGTTATTATTATTAGTACTATCCATGTCTGTAAATATAATACTCATTCTAAATTAAGAGATTCATCAACTATTTTTGCACAAATTAACAACTAAAAATAATTGACTATTTTTAATATCATTGATTAATTAAAATATAAATGAAAGGGAAATTTTAAAAAACCAATGTAGCATATACTTTTGCTGTAATTGATGTAATAAATATAGGCATGAGCTTGCGGGTGTAGTAACCGAAGTGGGAAGTAAAGTGGAGAAATTTAAAGTTGGAGACAAAGTAGGTGTGGGCTACTTGATTGATTCATGTCGATCATGCCAAGATTGTAATGACAATCTTGAGAATTACTGCCCCAAATTTGTAGTCACATGTGGTGCCAAGTATCGTGATGGAACCATCACATATGGAGGTTACTCCGACTCAATGGTTGCAGACGAGCACTTTGTGATTCGCATTCCTGACAACATACCTCTTGAATTTGCTGGTCCTCTCCTTTGTGCTGGTGTCACCGTATATAGTCCTCTTCGATTTTTTGGACTCGATAAGCCTGGTTTACATATAGGTGTTGTTGGTCTTGGTGGACTTGGCCATATGGCTGTGAAATTTGCCAAAGCTTTCGGTGCTAATGTGACCGTAATTAGTACATCGCCTAACAAAGAAAAGGAAGCAATAGAACACTTAGGAGCTGATTCATTTCTGATAAGTAGTGACCCAAAGCAGATTCAGGTACCATACAGTATATGATTAATTTGATTTTGAGATGCATTATTTCAGAAACTGACAATTATAATTCATAAACTAATATTATTGATAATATTTTAGGCTGCAATTGGTACTTTAGATGGTATTATTGACACCGTTTCAGCTGTTCATCCTCTCTTACCAATGATTGGTCTCTTGAAGTCTCATGGAAAGCTTATAATGCTTGGTGTAATAGTACAGCCTTTGCAGCTTCCTGAATATACTTTAATTCAAGGTAAGCATATGAAGAAAAATATTTCCATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATTCCCTTAGAATGGTTCAGGAGAAAATGCTAATTATACACTTCTATATGATTAAATATTTGAATCATGTATAATTTTATGCTCACTTTGTGTTAATTTCAAATTACTGATAGGGAGAAAAATATTAGCTGGAAGTCAAGTTGGAGGGTTAAAGGAGACTCAAGAAATGATTGATTTTGCGTCAGAACACAATGTAAAGCCGGATATTGAGGTTGTTCCGATTGATTATGTCAACACAGCAATGGAGCGTCTTGCTAAGGGAGACGTGAAATATCGATTTGTGATTGATATTGGAAACACATTGCAACCAAGCTCTTAA
Protein sequence

>MS.gene027819.t1

MATETKLVEHPKKAFGWAARDHSGVLSPFNFSRRETGEKDVAFKVLYCGICHSDLHMAKNEWGTSIYPLVPGHELAGVVTEVGSKVEKFKVGDKVGVGYLIDSCRSCQDCNDNLENYCPKFVVTCGAKYRDGTITYGGYSDSMVADEHFVIRIPDNIPLEFAGPLLCAGVTVYSPLRFFGLDKPGLHIGVVGLGGLGHMAVKFAKAFGANVTVISTSPNKEKEAIEHLGADSFLISSDPKQIQAAIGTLDGIIDTVSAVHPLLPMIGLLKSHGKLIMLGVIVQPLQLPEYTLIQGRKILAGSQVGGLKETQEMIDFASEHNVKPDIEVVPIDYVNTAMERLAKGDVKYRFVIDIGNTLQPSS